ABSTRACT Seeds of F1 hybrid cultivars of onions have been exclusively produced by utilising cytoplasmic male-sterility (CMS). A single locus, Ms, controls restoration of male-fertility of CMS onions. When a molecular marker tagging the AcPMS1 gene, a candidate gene for the Ms locus, was used to genotype diverse breeding lines, an unusual marker genotype showing an intermediate size of PCR product was

(2021). Obituary. The Journal of Horticultural Science and Biotechnology: Vol. 96, No. 3, pp. 407-408.

ABSTRACT This article studies the effect and mechanism of Si on drought and salt tolerance of Glycyrrhiza uralensis by focusing on the pathways of proline metabolism and nitrogen assimilation. G. uralensis was analysed under control, 70 mM NaCl, 15% PEG-6000 and 70 mM NaCl+15% PEG-6000 with or without Si. The results showed that Si increased P5Cs and δ-OAT activities by 16.29% and 5.85% under drought-salt

ABSTRACT Diospyros Kaki Cv (Qiyuezao) 117 is an early ripening persimmon cultivar, ready to be harvested in August. One of the pitfalls of this cultivar is the development of cracks on its surface. These cracks turn brown after astringency removal, preventing the commercialisation of this cultivar. Pre-harvest treatment with Superlon™ (containing benzyl adenine (BA) + gibberellic acid (GA4+7) at 40

ABSTRACT Understanding how strawberry (Fragaria x ananassa) nutritional and quality traits are genetically regulated and correlated is an essential step towards improving marker-assisted breeding programmes in this crop. A first step to achieve this goal was to construct a single nucleotide polymorphism (SNP)-based genetic map of 140 F1 individuals of a cross between the 2 octoploid parents ‘Redgauntlet’

ABSTRACT Our aim was to screen new hyper-variable mango SSRs (MSSRs) designed from Amrapali genome sequences to find polymorphic ones between Amrapali and Sensation parental genotypes. Out of 253 MSSRs screened against Amrapali and Sensation genotypes, 212 were polymorphic, of which 8 MSSRs showed aa, bb or bb, aa allelic patterns. MSSRs showing polymorphism in terms of homozygous for ‘a’ allele in

ABSTRACT In the modern era, technology is continuously and quickly growing. Nanotechnology is one such technology which is of great concern worldwide. It is basically the manufacturing of particles, materials and products within the nano-scale. At nano scale, properties of matter are altered due to reduced molecular size and changed interactions between molecules. Nano formulations enable smaller quantity

ABSTRACT Catharanthus roseus (Madagascar periwinkle) plants exhibiting typical begomovirus symptoms, i.e., leaf yellowing and curling were found in Lakshmangarh, Rajasthan, India. Symptomatic and healthy leaf samples were collected and subjected to DNA extraction and polymerase chain reaction, using universal primers conserved for the coat protein region of begomoviruses. The full length of viral DNA

ABSTRACT A comprehensive review of automation of irrigation based on volumetric soil water content (VSWC) in the framework of IoT (Internet of Things) is presented. The fundamentals of electromagnetic sensors based on soil dielectric permittivity and the techniques used for measuring the VSWC are briefly described. Factors affecting sensor performance that have to be considered for selecting the appropriate

ABSTRACT We examined the effects of partial rootzone drying (PRD) and regulated deficit irrigation (RDI) on some physiological and agronomical traits in tropically grown sweet orange (Citrus sinensis (L.) Osbeck). Seven different irrigation strategies were applied over three consecutive years and compared regarding their effects on leaf gas exchange, vegetative growth, fruit yield and quality and gene

ABSTRACT Rose is one of the most important ornamental crops of the family Rosaceae. It is a complex crop having a patchy genome with different ploidies (2× to 10×), majorly tetraploid. With advancements in technologies, Rosa species are also being explored to understand the genetic, genomics, and molecular mechanisms behind variations of the traits. Whole-genome sequence information for the two Rosa

ABSTRACT Floriculture provides tremendous scope and opportunities and has become a major ingredient of modern lifestyle. There has been a transition in the floriculture industry as a commercial business venture with high market value and real opportunities. This research study examines the recent trends in area, production and trade of floriculture in India and at international level. It also tries

ABSTRACT Pomegranate (Punica granatum L.) is a hardy crop packed with nutritional and medicinal benefits. Exploitation of wild pomegranate germplasm is necessary to meet the modern-breeding objectives of superior quality, high yield and resistance to biotic and abiotic stresses. Genetic diversity and genetic relatedness among 61 cultivars of six countries and 35 wild accessions of Himalaya (India)

ABSTRACT Petaloid stamens in Paeonia lactiflora constitute an important developmental phenomenon that explain double flower formation. Although research on stamen development in model plants has progressed considerably, the underlying molecular mechanism in P. lactiflora petaloid stamens is still unclear. In this study, acomparative transcriptomic analysis was performed on two cultivars of P. lactiflora

ABSTRACT Carya illinoinensis (pecan) is a fruit crop native to Mexico and the USA and cultivated across the world. Despite the advances of the molecular markers’ technologies and their use in genetic studies of diversity and breeding, studies with this species only reticently accompanied this progress. Here we review the studies using molecular markers, the gaps, and the perspectives concerning these

ABSTRACT Amaranth is rich in betalain. Both betalain metabolites and plant growth are affected by external factors. However, studies of amaranth seedling growth and betalain metabolism are limited by a lack of appropriate reference genes for qRT-PCR analyses. We selected 13 candidate reference genes from the amaranth transcriptome database and tested their expression stability under various environmental

ABSTRACT Phenylalanine ammonia-lyase (PAL) is a rate-limiting enzyme of phenylpropanoid biosynthesis in plants that synthesise many ubiquitous key compounds, including phenolic acids, flavonoids, lignins, and stilbenes. The first draft of the full grape genome assembly was released, but systematic analysis of the PAL family has not been completed in detail. In this study, 15 PALs were identified in

ABSTRACT Bend neck of scape and short vase life are the most important problems of cut gerbera flowers in postharvest stage. Inhibitors of laccase enzyme influence lignin formation at cut stem end of flowers. In this study, cut gerbera (Gerbera jamesonii cv. Intense) flowers were subjected to laccase enzyme inhibitors including Dithiothreitol (DTT: 0.05, 0.1, 0.2 mM), Thioglycolic acid (TGA: 0.25,

ABSTRACT Drought is an environmental stress that limits agricultural and horticultural production worldwide. Paeonia lactiflora Pall. is a perennial flowering plant with strong environmental adaptability and a good candidate for production in arid areas. Using the Illumina Hiseq platform to study the transcriptome of P. lactiflora leaves under drought stress, 62.27 Gb of clean data was obtained and

ABSTRACT Vacuolar processing enzymes (VPEs) are important cysteine proteases and function in the processing and maturation of protein precursors, plant senescence and immunity, programmed cell death, as well as sugar accumulation. This paper investigated VPEs in pear and identified as a key finding eight members of the VPE family in the pear (Pyrus) genome, and their gene structure, protein conserved

ABSTRACT Studies proving the food properties of some vegetable species are still incipient. Aiming to obtain deeper knowledge about the nutritional potentials, the scope of this work was to characterise the food compounds of interest, present in vegetables known as unconventional, in Brazil, and to group these species according to their chemical and nutritional similarities. Eight nutritional characteristics

ABSTRACT Papaya leaf curl disease (PaLCuD) is a serious problem to the production of papaya (Carica papaya L.) in India and infection due to this virus is not limited to papaya, but to chilli and tomato. In this study, leaf samples from diseased papaya plant showing extreme leaf curling and venation were collected from New Delhi region, India during the period of 2016–2017. Rolling Circle Amplification(RCA)

ABSTRACT Studies proving the food properties of some vegetable species are still incipient. Aiming to obtain deeper knowledge about the nutritional potentials, the scope of this work was to characterise the food compounds of interest, present in vegetables known as unconventional, in Brazil, and to group these species according to their chemical and nutritional similarities. Eight nutritional characteristics

ABSTRACT Pomegranate (Punica granatum L.), a fruit-bearing shrub with extensive genetic and geographical diversity, effectively takes its place among several other significant global fruit crops. The global demand for pomegranate is increasing consistently since this fruit has recently been merited with the status of a ‘super fruit’; apart from its other culinary and medicinal (both ethnomedical and

ABSTRACT The objective of this study was to investigate the changes of health-promoting bioactive compounds and related enzymes of ‘Hutai No.8’ grape (Vitis vinifera L.) in response to deficit irrigation. The experiment had five irrigation treatments: FI (75%–90% of FC [field water holding capacity] from budburst to full maturation), DI (same as FI except 50%–60% of FC from peasize to full maturation)

ABSTRACT Aril browning is a serious physiological disorder and major fruit quality anomaly in red-coloured pomegranates, under environmental stress such as temperature extremes. In this study, the single and combined effect of foliar nutrition with calcium, boron, and potassium on aril browning in ‘Rabab’ cultivar pomegranate (Punica granatumL.), were evaluated. Experimental factors were foliar nutrition

ABSTRACT Aril browning is a serious physiological disorder and major fruit quality anomaly in red-coloured pomegranates, under environmental stress such as temperature extremes. In this study, the single and combined effect of foliar nutrition with calcium, boron, and potassium on aril browning in ‘Rabab’ cultivar pomegranate (Punica granatumL.), were evaluated. Experimental factors were foliar nutrition

ABSTRACT Potato (Solanum tuberosum L.) under Mediterranean climate may be affected by internal brown spot, non-pathogenic disorder of flesh tissues leading to a dramatic depreciation of tubers. Its aetiology is still clarifying; however, some abiotic stressors, such as the high temperatures, heat and drought are noticed as crucial factors exacerbating the physiological decline. With the aim of escaping

An alternative approach to control Huanglongbing disease in Citrus is the development of transgenic plants expressing genes that may influence pathogen development. We report herein the production of sweet orange transgenic plants bearing gene constructs containing the antimicrobial gene attacin A (attA) under the control of phloem-specific promoters. Transcripts of the attA gene were accumulated in

ABSTRACT Orchid flowers have unique floral organisation which make them indispensable to the floral industry. The MADS-box gene family encodes for transcription factors that are involved mainly in floral organisation. This study was conducted on Vanilla planifolia, which is an important orchid that is commercially exploited by the food industry for a highly valued flavour, vanillin. V. planifolia transcriptome

ABSTRACT RNAi approach was attempted to impart resistance against Banana bract mosaic virus (BBrMV). An intron hairpin RNA (ihpRNA) vector targeting its replicase gene was constructed in pSTARLING vector. A fragment (421 bp) of the BBrMV replicase gene isolated from the infected sucker of banana cv. Nendran (Musa AAB) was used for making ihpRNA cassette. The ihpRNA cassette (NotI fragment) was mobilised

ABSTRACT Genetic engineering techniques offer opportunities for deliberate modulation and enhance the bio-functionality and bio-availability of horticultural crops, particularly fruit and vegetable which can aid in realising the goals of food and nutritional security for the burgeoning world population. The need of the hour calls upon us to use modern-day technologies such as genetic engineering in

ABSTRACT High throughput sequencing of small RNA and RT-PCR revealed that Vitis vinifera ‘Kyoho’, the main grapevine cultivar in Hangzhou, China, was co-infected with at least three viruses, namely grapevine leafroll-associated virus 3 (GLRaV-3), grapevine geminivirus A (GGVA), and grapevine fleck virus (GFkV), and five viroids, namely hop stunt viroid (HSVd), citrus viroid II (CVd-II), grapevine yellow

ABSTRACT In citrus, post-harvest treatment with ethylene or ethephon in an airtight container frequently resulted in fruit calyx drying, browning and abscission, with a consequent reduction in commercial value. To avoid these problems, pre-harvest degreening with ethepnon was performed on ‘CARA CARA’ navel orange fruits. An earlier and deeper orange colour development was obtained after treatment,

ABSTRACT Anthocyanins are the main pigments in leaves, flowers and fruits, performing diverse biological functions in plants and exhibiting benefits for human health. The purple-leaved accession of Brassicacarinata was used to study the biosynthetic mechanism of anthocyanins in this study. To elucidate the mechanisms of anthocyanin accumulation in the purple-leaved line, we employed comparative transcriptome

ABSTRACT Molecular tools were largely used to characterise native almond (Prunus dulcis Mill.) cultivars, to elucidate their diversification and to establish their membership to Mediterranean germplasm pools. Hereby, the efficiency of the application of PCR techniques to study the genetic diversity of S alleles in almond was tested to identify self-incompatible cultivar combinations and groups. Hence

ABSTRACT This study examined the effects of micro-bubble water and two types of light-emitting diode (LED) irradiation (blue and red-blue) on the growth and morphology of three lettuce cultivars (Lactuca sativa L.). The growth in the above-ground portions of the two cultivars was reduced by the use of micro-bubble water depending on the type of LED irradiation employed. In particular the leaf weight

ABSTRACT Hot air (HA) treatment has been demonstrated to control chilling injury (CI) in peaches effectively, however, little is known about the effects of HA on the changes of sugar and organic acid. In order to explore the relationship among CI susceptibility, soluble sugar and organic acid metabolism, peach fruit (Prunus persica) were treated with HA (40°C, 4 h) and then stored at 1°C for 35 days

ABSTRACT The QTL mapping for gynoecy in cucmber (Cucumis sativus L.)was carried out by using backcross populations (BC1F2 & BC1F3) of a cross using two inbred lines; one monoecious with multiple (≥ 5) lateral branching habit cv. Pusa Uday (Indian slicing type) and gynoecious exotic line Gy14 (American pickling type) with few lateral branches (1–2). QTL detection was performed by employing QTL Ici mapping

ABSTRACT DNA methylation plays key roles in regulating genes which control somatic embryogenesis (SE) in plant. 5-Azacytidine (5-AzaC) was considered as a common inhibitor of DNA methylation. Longan (Dimocarpus longan Lour.) SE has been established and used as model system for studying mechanism of SE in woody plants. However, effects of 5-AzaC on longan EC and the underlying mechanism at molecular

ABSTRACT Green-mature and turning cherry tomatoes (Solanum lycopersicum L.) of a red (‘Cherry Little Wonder’) and a yellow (‘Goldilocks’) cultivar (cv) were illuminated, post-harvest, with either red (118 µmol m–2 s – 1 at 638 nm) or blue LED light (118 µmol m–2 s – 1 at 454 nm) for 48 h and subsequently stored for up to 21 days at room temperature. The effects of the treatments on colour, firmness

ABSTRACT Biological herbicides or bioherbicides can reduce the use of synthetic herbicides and minimise the negative environmental impact of them. To be successful, bioherbicides must have a competitive price, efficiency and consistency. In this review, the highest level of development, and the future prospects for herbicides of biological origin are reported and discussed. Application of bioherbicides

ABSTRACT Biological herbicides or bioherbicides can reduce the use of synthetic herbicides and minimise the negative environmental impact of them. To be successful, bioherbicides must have a competitive price, efficiency and consistency. In this review, the highest level of development, and the future prospects for herbicides of biological origin are reported and discussed. Application of bioherbicides

ABSTRACT MicroRNA (miRNA) belongs to a class of non-coding small RNAs that post-transcriptionally regulate gene expression. In this study, we employed transcriptome, small RNA, and degradome sequencing to systematically investigate the miRNAs in Paeonia ostii. In total, 113 novel miRNA hairpin precursors were predicted, and 123 novel potential miRNAs were identified. Among them, five novel miRNAs and

ABSTRACT The study objective was to determine the effects of field application of hexanal on pre-harvest market attributes of orange (Citrus sinensis L.) fruits. The first factor was hexanal concentration (0.01, 0.02, 0.04% and controls – untreated fruits), the second factor consisted of time of hexanal application prior to fruit harvest (7, 21, 42 and 60 days to harvest) and the third factor was season

ABSTRACT The effects of controlling night temperatures (NTs) during summer-season on flowering and photosynthetic characteristics in Chrysanthemum morifolium were investigated at NTs of 20, 23, 26, or 29°C and a day temperature (DT) of 35°C. Days to budding and flowering increased more in the plants grown at NTs of 26 or 29°C than those of 20 or 23°C, regardless of cultivar. The flowers appeared as

ABSTRACT The Chinese water chestnut (Eleocharis dulcis (Brum.f.) Trin.) is conventionally propagated by underground corm with a lower propagation rate. In the present study, we attempted to develop a simple and efficient protocol for in vitro shoot regeneration, corm formation, and the generation of plantlets, on MS medium supplemented with BA, TDZ, NAA, and IAA. Additionally, the effect of BA on the

(2020). Journal of horticultural science and biotechnology – journal prize 2020. The Journal of Horticultural Science and Biotechnology: Vol. 95, No. 5, pp. 541-541.

ABSTRACT Our objective was to study the differentially expressed genes (DEGs) of apple (Malus. sp.) rootstock with different gravitropic setpoint angles (GSAs), during the formation of stem-root GSAs. The annual shoots of eight years hybrid seedlings of Malus pumila‘Ralls’ and M. sp. were used as experimental materials, and softwood cutting was carried out in mid-May. Different strains of adventitious

ABSTRACT Fruit skin colour in cherries (Prunus avium L.) is one of the most important market characteristics with customer preferences varying across cultures and countries. Based on skin colour, cherries can be divided into three market classes: yellow, blush, and mahogany. The PavMYB10.1 gene has been recently shown to be a key player in fruit colouration, and three alleles (PavMYB10.1a [WT(5A)];

ABSTRACT Dragon’s head (Lallemantia iberica Fisch. & Mey), annual herb from the Lamiaceae family, is worldwide distributed. Lallemantia is a medicinal plant with a variety of uses, and all of its parts have economic applications, including leaves to extract essential oils, mucilage and oil for different purposes in food and medicine. This study evaluated the effects of inoculants (single and dual application

ABSTRACT Catechins are the major compounds in tea (Camellia sinenesis L.) and are responsible for its sensory qualities and health benefits. The study investigated the response of catechins biosynthesis to temperature changes in the controlled conditions and the possible mechanisms. The low-temperature treatment inhibited the growth of young shoots, suppressed the expression of catechins biosynthesis-related

ABSTRACT Sweet potato is an important crop that produces starch, dietary fibre, β-carotene, and anthocyanin used as food and for industrial use. Recent studies have focused on the expression of genes involved in β-carotene and anthocyanin biosynthesis, and real-time quantitative PCR (RT-qPCR) has been the main technique used for assessing these genes. For RT-qPCR, suitable reference genes for gene

ABSTRACT Chilli (Capsicum species) is an important spice and vegetable crop of the world. Capsicum species have been widely used in food as well as in pharmaceutical industries. This is because these species possess various phytochemicals with antidiabetic and antioxidant activities. There has been a continuous global demand for chilli as a commercial crop due to its food value and health benefits

ABSTRACT Genome editing technology, which enables researchers to modify specific genomic loci, may be useful for accelerating the breeding of many fruit crops. The aim of this study was to evaluate the CRISPR/Cas9-mediated editing of the blueberry (Vaccinium spp.) genome. We first optimised the plant regeneration system to increase the genetic transformation efficiency for ‘Blue Muffin’ and ‘O’Neal’

ABSTRACT The aim of this work was to study the effect of postharvest light treatment as a potential degreening method on ‘Okitsu’ Satsuma mandarin (Citrus reticulata Blanco). Furthermore, a possible synergistic effect of light and ethephon treatment was also evaluated. For this, mature green fruit were harvested and treated. The treatments were: 1) exposing the fruit to increasing periods of time of

ABSTRACT In ‘Yali’ pears (Pyrus bretschneideri Rehd.) there is extra tissue formed on one side of the fruit, near the pedicel, causing a shape like a duck head termed Yatu, which is based on the Chinese phonetic alphabets of the raised duck head. The cell number of Yatu tissue has been known to be a direct factor influencing Yatu formation. The Yatu formation status among the fruits within an inflorescence

ABSTRACT A stable transformation protocol using Agrobacterium tumefaciens strain EHA 52 harbouring the recombinant plasmid pBI121-CycD2 for anther derived embryos of Eriobotrya japonica (Thunb.) Lindl. cv. ‘Dawuxing’ was established. Parameters optimised were the concentration of antibiotics and their combinations. Results showed that strain EHA 52 is completely not sensitive to carbenicillin at any

ABSTRACT The colour of fresh pods is one of the most important traits that affects the commercial value of cowpea (Vigna unguiculata) beans. To investigate the genetic architecture, we firstly scored the fresh pod colour of cowpea for six generations in field trials. Afterwards, an F2 population of 150 recombinant individuals was derived from crosses between two high-generation inbred cowpea lines

ABSTRACT In this study, attempts were made to confirm the origin of mango (Mangifera indica L.) embryos produced in vitro through somatic embryogenesis by using explants which were presumed as of diverse origin using SSR markers. SSR markers used in the study detected a total number of 148 alleles per locus with the mean number of alleles ranging from 8 alleles (MiKVR–81) to 11 alleles per SSRs (MiIIHR–21478)