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Dietary quercetin improved growth, body composition, haematology, immunity and resistance to Aeromonas hydrophila infection in common carp (Cyprinus carpio) Aquac. Res. (IF 2.0) Pub Date : 2022-11-13 Saade Abdalkareem Jasim, Reyadh H. Al-Mosawi, Kakhor Khalikov, Walid Kamal Abdelbasset, Irfan Ahmad, Shehla Shoukat, Mohammed Abed Jawad, Hafsan Hafsan, Yasser Fakri Mustafa, Maryam Norbakhsh
The present study was conducted to evaluate the growth, immunity, haematology, antioxidant components and resistances to Aeromonas hydrophila infection in the common carp, Cyprinus carpio fed quercetin (QUR) enriched diet. A number of 360 fish (30.5 ± 1.5 g) were distributed into 12 tanks (30 fish/tank) in three replicates and fed experimental diets containing 0% (control), 0.1% (1 g/kg diet), 0.5%
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Assessing the carrying capacity of Mercenaria mercenaria using a steady linear food web model in two pond-culture ecosystems Aquac. Res. (IF 2.0) Pub Date : 2022-11-07 Jie Feng, Tao Zhang, Hao Song, Zheng-lin Yu, Mei-jie Yang, Zhi Hu, Cong Zhou, Pu Shi, Wei Zhao, Liang Zhao, Peng-peng Hu, Pei-zhen Ma, Xiang-li Tian, Jian Liang, Yong-jun Guo, Hai-zhou Li, Wei-dong Li, Tao Bai
The Ecopath model was used to construct energy flow models of two pond ecosystems: Mercenaria mercenaria–fish–halophyte polyculture and M. mercenaria monoculture. Carrying capacities (CCs) of M. mercenaria were evaluated using energy flow models. Lateolabrax maculatus and M. mercenaria showed the highest biomass in the polyculture and monoculture ecosystems, respectively, and zoobenthos represented
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Analysis of combining ability and heterotic estimation on a complete Diallel cross involving five strains of common carp (Cyprinus carpio) from different geographical regions in West Java, Indonesia Aquac. Res. (IF 2.0) Pub Date : 2022-10-31 Didik Ariyanto, Odang Carman, Dinar T. Sulistyowati, Muhammad Zairin, Muhamad Syukur, Suharyanto, Yogi Himawan, Fandrianto S. Palimirmo
Decline in growth performance of Indonesian common carp was suspected due to the high rate of inbreeding. This condition requires a breeding programme to improve the genetic quality of the fish. This study was conducted to evaluate the combining ability and to estimate the value of heterotic in the hybridization of common carp. Five strains of common carp were crossed using a complete diallel cross
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Effect of salinity on growth, survival, haemato-biochemical and antioxidative status of Anabas testudineus (Bloch, 1792) juveniles reared in inland saline water Aquac. Res. (IF 2.0) Pub Date : 2022-10-30 Subam Debroy, Narinder Kumar Chadha, Satya Prakash, Paramita Banerjee Sawant, Vungurala Harikrishna, Mujahid A. Pathan, Ramjanul Haque, Prasanta Jana, Udipta Roy
A 60-day feeding trial was conducted to evaluate the growth, survival, physio-metabolic and haemato-biochemical responses of Anabas testudineus reared in (ISW) of varying salinity. Fingerlings (initial weight 6.55 ± 0.08 g) were randomly stocked in triplicate in five treatment groups viz., T1 (0 ppt), T2 (3 ppt), T3 (6 ppt), T4 (9 ppt) and T5 (12 ppt) following a completely randomized design (CRD)
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Evaluation of candidate reference genes for RT-qPCR analysis during developmental stages in striped murrel (Channa striata) Aquac. Res. (IF 2.0) Pub Date : 2022-10-28 Rakhi Kumari, Prem P. Srivastava, Kedar N. Mohanta, Rajesh Kumar, Paramananda Das, Lakshman Sahoo, Gargee Das, Priyanka C. Nandanpawar, Jackson Debbarma, Gopal Krishna, Gunnur M. Siddaiah
Real-time quantitative PCR (RT-qPCR) is the most advanced and commonly used method for quantification of gene expression levels because of its high sensitivity, specificity and accuracy. The analytical capability of RT-qPCR, on the other hand, is dependent on precise normalization by using the most stable reference genes. The present study was undertaken to find the best-suited reference gene for normalization
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Comparative proteome analysis reveals possible heterosis for growth, immunity and antioxidation mechanisms in Macrobrachium nipponense hybrid offspring and parent populations Aquac. Res. (IF 2.0) Pub Date : 2022-10-27 Yiming Li, Yucong Ye, Qichen Jiang, Xuan Che, Yunlong Zhao
Macrobrachium nipponense, an important economic aquaculture species in China, is experiencing degradation of shrimp germplasm resources. Hybridization is a means of genetic improvement and can contribute to overcoming this serious problem. However, the molecular mechanisms of heterosis resulting from hybridization is unknown. In this study, LC–MS/MS combined with TMT tags were used for comparative
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The effects of feeding frequency on the growth performance, body composition, health status and histology of juvenile meagre (Argyrosomus regius) Aquac. Res. (IF 2.0) Pub Date : 2022-10-27 Derya Güroy, Onur Karadal, Serhan Mantoğlu, Betül Güroy, Olcay Şimşek, Kamil Çelebi, Orhan Tufan Eroldoğan, M. Ayçe Genç, Ercüment Genç
This study evaluated how the feeding frequency impacts growth performance, haematological parameters, fatty acid profile and liver histology in meagre (Argyrosomus regius). Fish (15.48 ± 0.11 g) were fed to satiation with a diet formulated according to the nutritional requirements of the species (48% crude protein and 18% crude lipid) for 12 weeks in six different feeding frequencies; 1, 2, 3, 4, 5
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Fermented soybean meal can partially replace fishmeal and improve the intestinal condition of goldfish juveniles reared in a biofloc system Aquac. Res. (IF 2.0) Pub Date : 2022-10-26 Larissa da Cunha, Kayane Pereira Besen, Nandara Soares de Oliveira, Fernanda Regina Delziovo, Rafaela Gomes, Júlia Montibeller da Cruz, Fernanda Picoli, Enric Gisbert, Everton Skoronski, Thiago El Hadi Perez Fabregat
This study evaluated the effects of different dietary inclusion levels of fermented soybean meal (FSM) as replacement for fish meal and their effects on the productive performance and intestinal condition of goldfish (Carassius auratus) produced in biofloc (BFT) system. Five isoproteic (39.5% crude protein) and isoenergetic diets (4250 kcal kg−1 of crude energy) were formulated with FSM inclusion levels
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Effect on physiological responses and cultured pearl biology of different nuclei insertion positions in the visceral mass of freshwater pearl mussel (Hyriopsis cumingii) Aquac. Res. (IF 2.0) Pub Date : 2022-10-25 He Wang, Kai Huang, Shangle Feng, Xuenan Li, Luping Wang, Xingrong Xuan, Yuanshuai Fu, Zhiyi Bai, Wenjuan Li
The quality of pearls is related closely to the position where the nucleus is inserted. The visceral mass of freshwater pearl mussels has the physiological condition for the culture of pearls, but the appropriate position for inserting the nucleus has not been determined. In this study, the physiological responses and cultured pearl biology were used to explore the most suitable of five positions in
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Comparative study of blood physiological, antioxidant capacity, nutrition and organoleptic quality between wild, factory and cage-cultured Hexagrammos otakii Aquac. Res. (IF 2.0) Pub Date : 2022-10-25 Xin Gao, Ziru Chu, Lidong Shi, Haojie Zhai, Wei Wang, Tongjun Ren, Yuzhe Han
Nutrient richness and fast-growing rate have rapidly advanced the aquaculture of Hexagrammos otakii. The current farming methods of H. otakii include wild stocking, cage culture and factory culture; however, the influence of each style on this species is unclear. This study aimed to establish and compare the nutritional profiles, muscle quality and physiological status of wild (WF), factory-cultured
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Apparent digestibility of ten protein ingredients for largemouth bass (Micropterus salmoides) Aquac. Res. (IF 2.0) Pub Date : 2022-10-25 Hongjie Qiu, Min Dai, Naisong Chen, Songlin Li
The study was conducted to investigate the apparent digestibility coefficients (ADC) of nutrients in commonly used protein sources for largemouth bass. A reference diet and test diets with 70% reference diet and 30% feedstuff were formulated with yttrium oxide as a digestibility indicator. Overall, the ADC of dry matter of tested protein ingredients was 69.5%–96.4%, and the ADC of dry matter in animal
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The influence of salinity on egg incubation and early larval development of the flameback angelfish Centropyge aurantonotus Aquac. Res. (IF 2.0) Pub Date : 2022-10-24 Ksenia Skorupa, Raoani Cruz Mendonça, Sergio Leandro Araújo-Silva, Danillo dos Santos Santana, Jonathas Rodrigo dos Santos Pinto, Mônica Yumi Tsuzuki
Larval mortality constitutes the major bottleneck in Centropyge species aquaculture. Marine fish eggs and larvae are sensitive to abiotic conditions variations, as salinity. Captive natural spawning of Centropyge aurantonotus were used to evaluate effects of salinity on eggs and larvae up to 72 hours post hatch (hph). Eggs were transferred from 33‰ to 10‰, 15‰, 20‰, 25‰, 30‰, 35‰ and 40‰ salinity,
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Defatted black soldier fly larvae meal as a dietary ingredient for tambaqui (Colossoma macropomum): Digestibility, growth performance, haematological parameters, and carcass composition Aquac. Res. (IF 2.0) Pub Date : 2022-10-24 Driely Kathriny Monteiro dos Santos, Thiago Macedo Santana, Francisco de Matos Dantas, Ana Beatriz de Sena Farias, Cláudia Maiza Fernandes Epifânio, Armando Gomes Prestes, Flávio Augusto Leão da Fonseca, Giuliana Parisi, Elisabete Maria Macedo Viegas, Ligia Uribe Gonçalves
Black soldier fly (BSF, Hermetia illucens) meal is a prominent ingredient to keep up with the increasing demand of protein sources for aquafeeds. This study evaluated the digestibility of defatted BSF larvae meal (DBSFL) by indirect method, growth performance, haematological parameters, and carcass composition of tambaqui (Colossoma macropomum) fed diets containing DBSFL. The apparent digestibility
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Dietary protein requirement level of tinfoil barb (Barbonymus schwanenfeldii Bleeker 1853) fry Aquac. Res. (IF 2.0) Pub Date : 2022-10-24 Mohd Puaad Nuruljannah, Mohd Salleh Kamarudin, Fadhil Syukri, Clement R. de Cruz
An 8-week feeding trial was conducted to determine the optimal dietary protein requirement for tinfoil barb (Barbonymus schwanenfeldii) fry. Six isocaloric practical test diets (17 kJ g−1 gross energy) were formulated to contain 25%, 30%, 35%, 40%, 45% and 50% protein. Fry (0.40 ± 0.03 g) were randomly stocked in eighteen 60 L glass aquaria at 25 fish per aquarium. Each aquarium was fitted with an
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Marine cage culture leads to higher IMP content and ADSL gene expression in the muscle of Japanese sea bass (Lateolabrax japonicus) compared with freshwater pond culture Aquac. Res. (IF 2.0) Pub Date : 2022-10-24 Xuedi Du, Jiali Wu, Jie He, Xiaojing Dong, Yuanyuan Fu, Zeyu Shao, Longsheng Sun, Shuyan Miao
Why seawater-reared Japanese sea bass (Lateolabrax japonicus) tastes better than that reared in freshwater has not been well determined. Given that flavour nucleotides have great impact on meat flavour, we compared the contents of AMP, GMP and IMP in the back muscle of Japanese sea bass cultured in marine cage and freshwater pond. The results showed that IMP was the main kind of flavour nucleotide
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Stereological analysis of spermatogenesis in Brycon orbignyanus (Characiformes: Bryconidae) Aquac. Res. (IF 2.0) Pub Date : 2022-10-20 Cristiane Fernanda Benevente, Patricia Postingel Quirino, Luciane Gomes-Silva, Maria Luiza Ribeiro Delgado, Bárbara Correa Bianchini, Guilherme Antônio de Freitas, Alexandre Ninhaus-Silveira, Rosicleire Veríssimo-Silveira
Piracanjuba, Brycon orbignyanus, is an important rheophilic species for aquaculture studies on genetic diversity, spermatogenesis, semen cryopreservation and taxonomy. Due to anthropic actions its population has been declining, resulting in the loss of natural stocks, thus pushing the species to near extinction. No stereological analysis of spermatogenesis in piracanjuba has been performed. The present
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Vibrio load and percentage composition of sucrose-fermenting vibrios in Nile tilapia (Oreochromis niloticus) cultured in brackish water earthen ponds and biocontrol potential of recycled tilapia-conditioned greenwater against population growth of V. parahaemolyticus and non-sucrose-fermenting vibrios in the grow-out culture of white leg shrimp (Litopenaeus vannamei) Aquac. Res. (IF 2.0) Pub Date : 2022-10-20 Rolando Pakingking, Peter Palma, Evelyn Grace de Jesus-Ayson, Roselyn Usero
Vibrio are known opportunistic pathogens of pond-cultured fish and shrimp. This study determined the presumptive Vibrio count (PVC) and percentage composition of “beneficial” sucrose-fermenting vibrios (SFVs) and “pathogenic” non-SFVs in the water, sediment, gills and intestines of healthy tilapia Oreochromis niloticus collected every 2 weeks from Days 30 to 120 post-stocking in brackish water earthen
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Effects of dietary thiamin on flesh quality in grass carp (Ctenopharyngodon idella) Aquac. Res. (IF 2.0) Pub Date : 2022-10-19 Ling-Mei Wen, Feng Lin, Jiang Wei-Dan, Pei Wu, Liu Yang, Kuang Sheng-Yao, Shu-Wei Li, Tang Ling, Lu Zhang, Hai-Feng Mi, Xiao-Qiu Zhou
An 8 week experiment was conducted to assess the effect of dietary thiamin on meat quality of grass carp. Totally, 540 fish were randomly distributed into 18 cages (1.4 m × 1.4 m × 1.4 m) each containing 30 fish in three replicates. Test fish were fed with respective diets for four times daily: 0.12 (thiamin un-supplemented diet), 0.43, 0.83, 1.25, 1.62 and 2.04 mg thiamin/kg diet. Results revealed
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Effects of dietary protein level on growth, histology and digestive enzyme activities of ornamental fish Ancistrus cirrhosus Aquac. Res. (IF 2.0) Pub Date : 2022-10-19 Tolga Şahin, Mert Gürkan
Regardless of the developmental stage of the cultured organism, proper nutritional care is essential. A significant number of aquacultural species rely on protein as their main energy source and protein feeds sold in the ornamental market are formulated with widely varying protein inclusion levels. As Ancistrus cirrhosus is one of the most popular ornamental fish species, this study aimed to determine
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Using machine learning technique for disease outbreak prediction in rainbow trout (Oncorhynchus mykiss) farms Aquac. Res. (IF 2.0) Pub Date : 2022-10-18 Mesut Yilmaz, Mustafa Çakir, Okan Oral, Mükerrem Atalay Oral, Tülin Arslan
Water quality parameters such as temperature, dissolved oxygen, pH and total dissolved solids are important environmental factors affecting fish welfare. The deterioration of these parameters beyond the tolerance limits causes environmental stress and suppression of the immune system. Moreover, it allows opportunistic pathogens that are always present in the environment to infect immune-suppressed
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Identification, characterization and nutritional regulation of fatty acid-binding protein (fabp) genes by vegetable oils in European seabass (Dicentrarchus labrax) reared in low water temperatures Aquac. Res. (IF 2.0) Pub Date : 2022-10-17 Oğuz Taşbozan, Celal Erbaş, Mehtap Bayır, Erdal Özdemir, Abdulkadir Bayır
Dietary n-3 highly unsaturated fatty acids (HUFAs), which are low in vegetable oils (VO), are natural ligands of fatty acid-binding protein (fabp) genes, which function in molecular n-3 HUFAs metabolism in fish. Low water temperature (LWT) also affects fish fatty acid (FA) metabolism. Therefore, in this study, we investigated mRNA expression levels of fabp genes in European seabass (Dicentrarchus labrax)
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Effects on grass carp (Ctenopharyngodon idella) induced by waterborne lead exposure and the association with alteration of microbiota in the intestine and water Aquac. Res. (IF 2.0) Pub Date : 2022-10-17 Yudong Du, Haisu Liu, Biao Han, Yang Lei, Kun Qian, Anli Wang, Shengli Fu
Heavy metal lead (Pb) is a primary pollutant that pollutes aquatic environments and can be enriched in aquatic animals. Here, the effects of waterborne Pb on grass carp (Ctenopharyngodon idella) within 96 h of exposure were detected, and the association between microbiota alterations in the intestines and water was analysed. Results revealed that the intestinal barrier was damaged, including the intestinal
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Bimonthly variation in nutrient composition and taste components of Crassostrea gigas cultured in Rushan, Southern yellow sea Aquac. Res. (IF 2.0) Pub Date : 2022-10-17 Xinnan Zhao, Jixing Peng, Haiyan Wu, Guanchao Zheng, Mengmeng Guo, Xuming Kang, Zhijun Tan
Oyster (Ostrea gigas thunberg) has high nutritional and economic values. We analysed bimonthly nutrient-level variations in Rushan oyster (Crassostrea gigas) to establish a basis for the analysis of the nutritional quality of oysters. The contents of the proximate composition, fatty acids, amino acids, minerals and non-volatile taste substances were measured using Pacific oysters collected between
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Assessment of open-source fish-free feeds (F3) for pompano, Trachinotus carolinus (Perciformes, Carangidae), under hyposaline conditions Aquac. Res. (IF 2.0) Pub Date : 2022-10-13 Kelly B. Alfrey, Delbert M. Gatlin, Frederic T. Barrows, Ewen McLean
Florida pompanos (15.3 g initial average weight) were fed one of seven open-source experimental diets to examine the effects of the complete removal of fishmeal (FM) and fish oil (FO) upon growth over a 12-week period. Diets were compared against an investigational feed containing FM and FO. Three different oils, derived from flax, canola and an alga, alone or in combination, were evaluated as alternatives
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Use of recirculation and settling chamber in synbiotic multi-trophic culture of Crassostrea sp. with Litopenaeus vannamei Aquac. Res. (IF 2.0) Pub Date : 2022-10-11 Priscilla Celes Maciel de Lima, Allyne Elins Moreira da Silva, Danielle Alves da Silva, Carlos Yure Barbosa de Oliveira, William Severi, Luis Otavio Brito, Alfredo Olivera Gálvez
The objective of this work was to evaluate the use of recirculation and settling chamber in synbiotic multi-trophic culture of Crassostrea sp. with Litopenaeus vannamei. Four treatments were tested: MONO (shrimp monoculture); integrated multi-trophic aquaculture (IMTA) (shrimp and oysters in the same tank); IMTA-R (shrimp and oysters in adjacent tanks with recirculation) and IMTA-RS (shrimp and oysters
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Selection of internal reference gene for quantitative real-time PCR analysis of gene expression in largemouth bass (Micropterus salmoides) under different experimental conditions Aquac. Res. (IF 2.0) Pub Date : 2022-10-11 Jing Qian, Junying Zhang, Yajie Mei, Mingyan Shi, Fan Wang
The study of gene function in largemouth bass requires accurate normalization by use of suitable internal reference genes. The expression levels of four candidate internal reference genes including β-actin, EF1-α, cytb and 12SrRNA in different tissues, two different developmental stages, and different experimental conditions of largemouth bass were evaluated by quantitative real-time PCR (qPCR) method
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Physiological and gene expression responses of diploid and triploid Pacific oyster (Crassostrea gigas) to heat acclimation Aquac. Res. (IF 2.0) Pub Date : 2022-10-07 Yongguo Li, Chengxun Xu, Qi Li
High temperature is considered to be one of the important causes of mass mortality of diploid and triploid oysters in summer. In order to compare the tolerance adaptability of diploid and triploid oysters to heat stress, the activities of superoxide dismutase activity (SOD), catalase activity (CAT) and contents of malondialdehyde (MDA) and the expression of Hsp70 and metallothionein (MT) genes in diploid
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Effects of microalgal diet on growth, survival and fatty acid composition of larvae rock oyster Striostrea prismatica (Gray, 1825) Aquac. Res. (IF 2.0) Pub Date : 2022-10-06 Daniel Rodríguez-Pesantes, César Lodeiros, Jormil Revilla, Adrián Márquez, Luis Freites, Stanislaus Sonnenholzner
The biochemical nature and composition of fatty acids (FA's) in microalgae influence the nutritional quality and efficiency of this food source in bivalve larviculture. This research analyses the effect FA's content of Chaetoceros gracilis and Tisochrysis lutea on the FA's composition, growth and survival of Striostrea prismatica larvae. We analysed the FA's profiles of the microalgae, and the tissue
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Integrating indoor and outdoor breeding systems to achieve year-round seed production in Pearlspot cichlid, Etroplus suratensis Aquac. Res. (IF 2.0) Pub Date : 2022-10-06 Binu Varghese, Grace George, Soundararajan Thiruvarasu
Pearlspot, Etroplus suratensis naturally thrives in the estuarine reaches of Southern peninsular India and Srilanka. The relative ease of adaptation to diverse habitats makes it a promising candidate for climate-resilient aquaculture. This potential remains underexplored due to limitations in the current seed production practices. The present study focused on sustainable seed production of this species
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Assessing the influence of the inclusion of Bacillus subtilis AQUA-GROW® as feed additive on the growth performance, feed utilization, immunological responses and body composition of the Pacific white shrimp, Litopenaeus vannamei Aquac. Res. (IF 2.0) Pub Date : 2022-10-06 El-Sayed Hemdan Eissa, Norhan H. Ahmed, Ashraf A. El-Badawi, Mohammad Bodrul Munir, Omayma M. Abd Al-Kareem, Moaheda E. H. Eissa, Elamier H. M. Hussien, Salah El-Sayed Sakr
This study was carried out to determine the influence of four concentrations of dietary Bacillus subtilis on the growth performance, nutrient utilization, immunity and antioxidants activities, mortality rate and chemical composition of Litopenaeus vannamei. Three hundred and sixty L. vannamei (4.00 g ± 0.03) were raised in 12 hapas (1 m3 each, placed in 4 ponds) equally distributed on apparently 38
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Effects of traditional Chinese medicines on biochemical parameters, antioxidant capacity and heat shock protein expression in rainbow trout (Oncorhynchus mykiss) under heat stress Aquac. Res. (IF 2.0) Pub Date : 2022-10-02 Shaoxia Lu, Ting Xian, Di Wang, Chang’an Wang, Yang Liu, Hongbai Liu, Shicheng Han
This study investigated the effects of the traditional Chinese medicines (TCMs) Huoxiangzhengqi decoction (HXZQD), Xiaochaihu decoction (XCHD) and Yinchenhao decoction (YCHD) on the biochemical parameters, antioxidant capacity and heat shock protein expression in rainbow trout (Oncorhynchus mykiss) under heat stress. The results showed that before stress, the serum glutathione peroxidase (GSH-Px),
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Identification of gonadal associated genes in black tiger shrimp (Penaeus monodon) using transcriptome analysis and high-throughput sequencing Aquac. Res. (IF 2.0) Pub Date : 2022-09-30 Yundong Li, Falin Zhou, Ningning Su, Song Jiang, Qibin Yang, Jianhua Huang, Lishi Yang, Shigui Jiang
Black tiger shrimp (Penaeus monodon) is a commercially important species. It is reported to have low reproductive maturation rates in captivity. To improve the production of this species via sustainable aquaculture, genetic techniques are required to improve its maturation rates. However, to date, reports on P. monodon reproduction at a transcriptional level are limited. To address this, we conducted
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Effects of supply ratio of chicken manure extract and yeast solution on reproductivity and culture conditions of euryhaline rotifers (Brachionus plicatilis) Aquac. Res. (IF 2.0) Pub Date : 2022-09-29 Tian Zhu, Xiang Xu, Shichen Xu, Ye Liang, Yifan Ding, Cui Han, Jiaxin Yang
To establish the optimal ratios of chicken manure extract (CME) and suspending yeast solution (SYS) for rearing rotifers, the population density, population growth rate, fatty acid compositions of rotifers and the unionized ammonia (NH3-N) and total phosphorus (TP) concentrations in cultured water under different ratios of CME and SYS (BG, EG1, EG2, EG3, EG4 and EG5) were investigated. The results
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Growth performance and immune responses of pengba (Osteobrama belangeri) during high-density fingerling rearing in biofloc system Aquac. Res. (IF 2.0) Pub Date : 2022-09-27 Pratap Chandra Das, Abhijit Nayak, Subhas Sarkar, Pushpa Choudhary, Rakhi Kumari, Snatashree Mohanty
High-density fingerling rearing of pengba was conducted for 90 days in biofloc system (BFS) to study the effect of stocking density on growth, feed utilization, water quality, water use efficiency and health of animals. The study was conducted in 12 continuously aerated tanks (50 m3), grouped into four treatments T-1, T-2, T-3 and T-4, which were stocked with pengba fry (1.25 ± 0.05 g, 4.74 ± 0.70 cm)
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Impact of phyto-piscicides, mahua (Bassia latifolia) oil cake (MOC) and castor (Ricinus communis L.) bean cake (CBC) upon the mineralizing heterotrophs in fish culture system Aquac. Res. (IF 2.0) Pub Date : 2022-09-26 Bithilekha Mondal, Shib Kinkar Das
Comparative impact of two phyto-piscicides viz. mahua (Bassia latifolia) oil cake (MOC) and castor (Ricinus communis L.) bean cake (CBC) upon the mineralizing heterotrophs was tested in outdoor experimental tanks. Following a sharp decline, aerobic heterotrophs and cellulose decomposing bacterial population recovered and the population density remained significantly higher than the pre-treatment level
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Dietary dried periphyton can improve growth, digestive enzyme, serum biochemical, antioxidant response and intestinal morphometric of Nile tilapia Aquac. Res. (IF 2.0) Pub Date : 2022-09-25 Rukaia S. Saleh, Eman Y. Mohammady, Ehab El-Haroun, Mohamed S. Hassaan
The present trial was conducted to investigate the effect of inclusion of dried periphyton in Nile tilapia diets on growth, digestive enzymes activity, haematological, oxidative stress and intestinal morphometric parameters. Five isoproteic (32%), isolipidic (5%) experimental diets were formulated, to comprise graded levels of dried periphyton (0, 2.5, 5, 7.5 and 10 g kg−1 diet). At the end of the
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Evaluating non-lethal and latent effects of the anti-sea lice pesticide azamethiphos on larvae and adults of the American lobster (Homarus americanus) Aquac. Res. (IF 2.0) Pub Date : 2022-09-25 Benjamin de Jourdan, Tillmann Benfey, Les Burridge, Marc Surette, Anne F. McCarthy, Aura M. Barría-Araya, Dounia Daoud
Both the American lobster fishery and salmon aquaculture are important to the economy of Atlantic Canada. Where these industries operate in proximity, research is needed to better understand any interactions and their possible effects. This study explored the effects of azamethiphos on survival and development of larval American lobsters, Homarus americanus, using exposures that reflect concentrations
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A study on the effects of light intensity on juveniles of the red claw crayfish Cherax quadricarinatus Aquac. Res. (IF 2.0) Pub Date : 2022-09-23 Jiang Wen-ping, Cheng Shun, Jia Yong-yi, Chi Mei-li, Liu Shi-li, Zheng Jian-bo, Li Fei, Liu Yi-nuo, Gu Zhi-min, Wang Dan-li
To study the effect of light intensity on red claw crayfish juveniles, experiments were carried out in this study. The results showed the following. (1) The 20 lux group had the highest survival rate. (2) The growth rates of the 20 lux, 200 lux and 0 lux groups were faster. (3) The juveniles in the 1000 lux and natural light groups were always clustered, while other groups were dispersed within 2–3 days
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The effect of total suspended solids on the nursery of Penaeus vannamei nauplius based on biofloc technology system Aquac. Res. (IF 2.0) Pub Date : 2022-09-23 Wenchang Liu, Yanshuo Guo, Shuang Li, Guozhi Luo, Hongxin Tan
A 14-day study was carried out to investigate the nursery of the nauplius (N5) Penaeus vannamei in biofloc technology (BFT) system. The whole experiment was divided into three groups: the control group (GC) without bioflocs, T300 and T600 with bioflocs in which total suspended solid (TSS) levels were maintained at 300 and 600 mg/L, respectively. The results revealed that the average total ammonia nitrogen
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Dietary β-sitosterol supplementation enhanced intestinal immune function of large yellow croaker (Larimichthys crocea) infected with Aeromonas hydrophila Aquac. Res. (IF 2.0) Pub Date : 2022-09-22 Yan Song, ZhiYuan Lu, XiaoFang Zhang, Jing Xu, Miao Gong, LingTing Meng, Zhiyuan Gong, Bin Zheng
An 80-day feeding trial was performed to investigate the effect of dietary β-sitosterol supplementation on intestinal immune function in large yellow croaker. 540 fish (initial average weight: 189.74 ± 0.31 g) were fed six diets containing graded levels of β-sitosterol (0.0%, 0.5%, 1.0%, 1.5%, 2.0% and 2.5%) and then challenged with Aeromonas hydrophila for 10 days. Results demonstrated that dietary
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Effects of diluted seawater on the growth, antioxidation capacity and accumulation of bioactive compounds of the brown alga, Sargassum muticum Aquac. Res. (IF 2.0) Pub Date : 2022-09-22 Fang Lü, Meili Xin, Liuqing Tang, Dongmei Zhan, Gang Ding, Haiyi Wu
Environmental condition is a significant factor that directly affects the growth, metabolism and productivity of marine algae. This study examined the influences of diluted seawater on the growth, antioxidation capacity and accumulation of bioactive compounds of the marine macroalga, Sargassum muticum. Compared to the natural seawater (30 ppt, parts per thousand) control, the specific growth rate was
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Growth performance and mineral composition of the white shrimp Penaeus vannamei and the sea grape Caulerpa lentillifera in a co-culture system Aquac. Res. (IF 2.0) Pub Date : 2022-09-22 Alexia Omont, Alberto Peña-Rodríguez, Shuma Terauchi, Ayako Matsui, Francisco Magallón-Barajas, Erika Torres-Ochoa, Masato Endo
Integrated systems have been proposed as a sustainable solution to minimize the environmental impact of shrimp intensive aquaculture practices. The increasing demand for seafood is largely attributed to a growing need for healthy food recommended in a human balanced diet, but information on the nutritional quality of the resulting products is still scarce. In this study, a co-culture system (CO) of
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Rapid quantitation of feed rotifer (Brachionus plicatilis) fatty acids by Raman spectroscopy Aquac. Res. (IF 2.0) Pub Date : 2022-09-20 Daniel P. Killeen, Jeremy S. Rooney, Asli Card, Liam B. Hegarty, Warren W. Fantham, Keith C. Gordon, Damian Moran
Improving the lipid composition of feed-rotifers enhances the survival and growth rates of juvenile fish in aquaculture, but monitoring enrichment profiles using gas chromatography (GC) is slow, labour-intensive and requires expensive instrumentation. Here, we describe the potential of Raman spectroscopy as an alternative, more rapid tool for quantitation of rotifer fatty acid composition in an aquaculture
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Reducing the feed input per unit of plant area as a means to improve the efficiency of sea asparagus and Pacific white shrimp biofloc technology-based aquaponics Aquac. Res. (IF 2.0) Pub Date : 2022-09-20 Joaquim Soares, Mateus Aranha Martins, Leonardo Castilho-Barros, Carlos Manoel do Espírito Santo, Felipe do Nascimento Vieira, Walter Quadros Seiffert
This study aimed to evaluate different feeding rate ratios in a biofloc technology-based aquaponics system employing sea asparagus (Sarcocornia ambigua) and Pacific white shrimp (Litopenaeus vannamei) in an 83-day experiment. Two treatments were evaluated: 100 and 50 g feed m−2 day−1, which were achieved by doubling the plant production area from 0.4 m2 to 0.8 m2. Shrimps (1.39 g ± 0.06 g) were reared
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Broodstock development, breeding and larval rearing of Acanthopagrus berda (Forsskal, 1775), a suitable species for farming in tropical waters Aquac. Res. (IF 2.0) Pub Date : 2022-09-19 Padinhate Purayil Suresh Babu, Anirudhan Anuraj, Madathampady Thomas Shilta, Pillaru Kandiyil Asokan, Kavungal Vinod, Narayan Dube Praveen, Kodi Srinivasa Rao, Narayan Gopalkrishna Vaidya, Suresh Mhaddolkar Sonali, Mahendra Pal, Jayasree Loka, Bhaskaran Pillai Santhosh, Nikarthil Sidhick Jeena, Joseph Imelda, Ignatius Boby, Achamveetil Gopalakrishnan
Standardization of seed production of the Picnic seabream, Acanthopagrus berda, was taken up in captivity. Broodstock development of A. berda was done in 6 m diameter galvanized iron cages during September 2020 to March 2021 using commercial feed (40% protein) supplemented with vitamins and minerals. Males with oozing milt were available consistently throughout the period while asynchronized maturation
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The effects of different diet, salinity and light condition on growth performance and moulting cycle of juvenile mud crab, Scylla paramamosain Aquac. Res. (IF 2.0) Pub Date : 2022-09-19 Yin Zhang, Zhi Huang, Yao Zhou, Haiyi Ma, Hafiz Sohaib Ahmed Saqib, Qiong Su, Wenxiao Cui, Hongyu Ma
Scylla paramamosain is an importantly economical mariculture crab. To figure out the optimized conditions in the culture of juvenile crabs, three experiments were conducted to examine the effects of diet, salinity and light condition on the growth performance (body weight gain, BWG; carapace width gain, CWG; carapace length gain, CLG) and moulting cycle (MC) of crablets from V to VII stages. The growth
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Identification SNPs in vitellogenin gene and their association with ovarian development and growth of Macrobrachium nipponense Aquac. Res. (IF 2.0) Pub Date : 2022-09-18 Sufei Jiang, Yiwei Xiong, Zixi Xia, Jisheng Wang, Wenyi Zhang, Dan Cheng, Yongsheng Gong, Yan Wu, Hui Qiao, Hongtuo Fu
In this study, the correlation analysis between gonadosomatic index and growth traits of M. nipponense was carried out for the first time. The results proved that short sexual maturity M. nipponense had a significant negatively effect on the female prawn size (p < 0.01) but had almost no effect on the male (p > 0.05). The full-length Vitellogenin gene of M. nipponense (Mn-Vg) were applied to detect
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A study on the effect of using magnetized water in dilution of the milt of Black Sea trout (Salmo trutta labrax) on sperm motility Aquac. Res. (IF 2.0) Pub Date : 2022-09-18 Mohmmed Hassan Ahmed, İlker Keskin, Suat Özkorucuklu, Aygül Ekici
Sperm motility is an important character for the assessment of milt quality and indicator to the expected rate of fertilization. Many factors can affect the sperm motility, including nature of water used as sperm activator medium after stripping the milt from the male fish. The aim of this study was to gain motility to spermatozoa and improve sperm kinematic parameters by applying a non-chemical method
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Yinchenhao decoction alleviates high-carbohydrate diet-induced hepatic lipids deposition by strengthening lipids metabolism and transport in largemouth bass Aquac. Res. (IF 2.0) Pub Date : 2022-09-18 Ji Liang, Liulan Zhao, Chenxin Gong, Qiao Liu, Yifan Hu, Hao Liu, Jiazhong Guo, Rui Huang, Zhiqiong Li, Song Yang
Yinchenhao decoction (YCHD) is a famous traditional Chinese medicine for improving hepatic metabolism disorder. The aim of this study was to investigate the effects of YCHD on lipid metabolism of juvenile largemouth bass fed on high-carbohydrate diet. Triplicate groups of fish (5.6 ± 0.2 g) were fed six experimental diets (one low-carbohydrate diet and five high-carbohydrate diets with gradients YCHD)
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New indices for the diagnosis of fish welfare and their application to the grass carp (Ctenopharyngodon idella) reared in earthen ponds Aquac. Res. (IF 2.0) Pub Date : 2022-09-18 Ana Silvia Pedrazzani, Camila Prestes dos Santos Tavares, Murilo Quintiliano, Nathieli Cozer, Antonio Ostrensky
Animal welfare is an issue of increasing importance in global aquaculture. However, the incorporation of this issue into routine aquaculture operations is a challenge. A possible path to change this is the recognition, identification and addressing of the ecological, physiological, nutritional, behavioural and psychological needs of fish. The present study uses grass carp (Ctenopharyngodon idella)
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Short-term exposure to 5‰ and 15‰ salinity causes the dynamic changes of the NKA gene, enzyme activities and morphological characteristics in fish tissues of Gymnocypris przewalskii Aquac. Res. (IF 2.0) Pub Date : 2022-09-18 Shen Huang, Changzhong Li, Zixuan Li, Droma Duanzhi, Yanhui Liu, Fengxia Ran, Jiawei Ding, Weiyi Yan, Chunyan Jia, Zhiyan Zhang, Hongfang Qi, Ying Luo, Yang Wang, Wenjie Jin
Salinity affects the physiological activities of aquatic animals and determines the distribution of fish. In this study, we found that the naked carp survived under 0–15‰ salinity, but survival rates decreased to varying degrees under 20‰ and 25‰ salinity, which indicates that the safety threshold of salinity tolerance of Gymnocypris przewalskii is 0–15‰. Next, the response rate of NKAα1 in the gills
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A cross-over experiment for on-farm evaluation of a novel pond aquaculture aeration device Aquac. Res. (IF 2.0) Pub Date : 2022-09-18 Sacha Ruzzante, Rezaul Hoque, Muhammad Meezanur Rahman, Amy M. Bilton
On-farm experimentation is an essential part of aquacultural product development, but experimental designs that are commonly used for intensive commercial aquaculture are usually not suitable for earthen pond aquaculture, particularly in smallholder contexts. Guidance for on-farm testing of aeration technologies is similarly lacking. We therefore propose an experimental design (a cross-over experiment)
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Metal ions effect on L-carnitine activity: A new research direction with a possible mitigating effect of dietary cellulose Aquac. Res. (IF 2.0) Pub Date : 2022-09-17 Phillip M. Tjale, Abdallah Ghonimy, Dong M. Zhang, Qiu J. Wang, Yu K. Chen, Yue Ma, Zhi X. Guo, Yun L. Zhao, Mohammed Hamdy Farouk
Disturbance of L-carnitine (LC) activity dissipates metabolic energy production in living cells. LC deficiency regards to malabsorption and biosynthetic and transport deficiencies, either due to dietary or metabolic factors. Metal ions interact with LC molecule or its metabolic system elements such as building blocks (lysine and methionine), biosynthetic enzymes (trimethyllysine hydroxylase, TMLH;
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Pm-miR-29b is involved in nacre formation by regulating tyrosinase-like protein in Pinctada martensii Aquac. Res. (IF 2.0) Pub Date : 2022-09-16 Xinlei Li, Ming Zhang, Min Yang, Rongrong Tian, Yuewen Deng, Yu Jiao
The miR-29 family includes miR-29a, miR-29b and miR-29c. Our previous research showed that miR-29a of Pinctada martensii (Pm-miR-29a) is involved in nacre formation via targeting neuropeptide Y receptor type 2 (Y2R). In this report, we validated and corrected the precise sequence of mature Pm-miR-29b and elucidated its function and mechanism difference with Pm-miR-29a in nacre formation. Our results
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Cage culture of tropical eels, Anguilla bicolor pacifica and A. marmorata juveniles: Comparison of growth, feed utilization, biochemical composition and blood chemistry Aquac. Res. (IF 2.0) Pub Date : 2022-09-15 Frolan A. Aya, Luis Maria B. Garcia
This study examined the performance and suitability of two tropical anguillid eels, Anguilla bicolor pacifica and A. marmorata, under cage culture conditions. Juvenile eels (1.73 ± 0.14 g body weight) of each species were stocked in triplicate hapa net cages (1 × 1 × 1.5 m; 30 eels/cage) suspended in outdoor tanks. Growth, feed utilization, biochemical composition and blood chemistry of the two species
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Nutritional potential of organic matter for benthic macrofauna in the surface sediments of the East China Sea from the perspective of essential amino acids Aquac. Res. (IF 2.0) Pub Date : 2022-09-15 Kui Wang, Haiyan Jin, Qicheng Meng
The purpose of this study was to explore whether essential amino acids (EAAs) in the particulate and sedimentary organic matter (OM) can potentially limit the nutritional needs for benthic macrofauna of different phyla, and which factors regulate the nutritional value with respect to EAAs in a large river-dominated shelf sea, the East China Sea. The EAA excess percentage of threonine, leucine, phenylalanine
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Effect of feeding frequency on growth performance, feed transit and digestive enzyme activity of Acipenser dabryanus juveniles Aquac. Res. (IF 2.0) Pub Date : 2022-09-14 Min Guan, Dezhi Zhang, Xiangang Sun, Debin Shu, Jun Rao, Daming Tang
Dabry's sturgeon (Acipenser dabryanus) is critically endangered in China. However, the lack of research on the feeding strategy of this species is detrimental to its conservation management. The present study evaluated the effects of feeding frequency (1, 2, 3, 4 and 5 times per day) on the growth performance, feed transit and digestive enzyme activity of juvenile Dabry's sturgeons (initial weight
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Effects of animal protein feedstuffs partially replacing fishmeal in the diet of Macrobrachium nipponense Aquac. Res. (IF 2.0) Pub Date : 2022-09-14 Qun-Lan Zhou, Yiwei Xiong, Peng Li, Momodou Saidyleigh, Chang Yang, Jingyuan Li, Cunxin Sun, Xiaochuan Zheng, Bo Liu, Sufei Jiang, Hongtuo Fu
Animal protein feedstuffs, including poultry by-product meal (PBM), spray-dried porcine solubles (SPS), hydrolysed feather meal (HFM) and spray-dried egg product (SEP), were used to replace 5% fish meal (FM) in the diet of Macrobrachium nipponense, respectively, to evaluate their effects on growth performance. A diet containing 25% FM was used as the control treatment. The various diets were fed to
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Effects of a commercial feed additive (Sanacore® GM) on water quality, productive performance and blood profile of gilthead seabream (Sparus aurata) Aquac. Res. (IF 2.0) Pub Date : 2022-09-14 Mohsen Abdel-Tawwab, Talal A. M. Abo Selema, Ashraf M. Soliman, Sherien H. H. Shady, Riad H. Khalil
The present study evaluated the effects of the inclusion of commercial feed additive (Sanacore® GM; SAN) in diets on growth and productive performance, digestive enzymes, intestinal microbiota, intestinal histomorphometry and blood profile of gilthead seabream (Sparus aurata). For this purpose, four diets containing 0.0% (the control), 0.1%, 0.2% and 0.4% SAN were formulated and offered to triplicate
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Effect of dietary C-Phycocyanin on growth, survival, haematology, immune response, gut microbiome and disease resistance of Pacific white shrimp, Penaeus vannamei Aquac. Res. (IF 2.0) Pub Date : 2022-09-13 Sandeep Kizhakkekarammal Puthiyedathu, Jesudhas Raymond Jani Angel, Sivaramakrishnan Thirugnanamurthy, Sudhin Suresh, Suganya Nathamuni, Ramalingam Ananda Raja, Sujeet Kumar, Sherly Tomy, Jagabathula Syama Dayal, Balasubramanian Changaramkumarth Paran, Paramita Banerjee Sawant, Shekhar Mudagandur Shashi, Chadha Narinder Kumar, Ambasankar Kondusamy
C-Phycocyanin (CPC) was extracted from Arthrospira sp. and isolated from an estuarine ecosystem in southern India. A 45-day feeding trial was conducted to unravel the effect of dietary CPC in Penaeus vannamei. Seven iso-nitrogenous and iso-energetic experimental diets were prepared with different levels of CPC at 0 (CPC0), 50 (CPC50), 100 (CPC100), 200 (CPC200), 400 (CPC400), 800 (CPC800) and 1600