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Gonadal expression profiles of sex-specific genes during early sexual differentiation in Japanese eel Anguilla japonica Fish. Sci. (IF 1.173) Pub Date : 2021-01-12 Hiroyuki Inaba, Seiji Hara, Moemi Horiuchi, Shigeho Ijiri, Takeshi Kitano
The Japanese eel Anguilla japonica is a prominent and highly valued species in the aquaculture industry in Japan. Sex determination and sex differentiation in eels are significantly affected by environmental factors; however, the molecular mechanisms involved in sex differentiation in eels are largely unknown. In this study, to investigate the gonadal expression profiles of sex-specific genes during
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Variations in life-history traits and statolith shape for Sepioteuthis spp. in the waters off southwestern Japan Fish. Sci. (IF 1.173) Pub Date : 2021-01-09 Tzu-Yun Ching, Chih-Shin Chen, Naoki Yagishita, Atsuko Yamaguchi, Chia-Hui Wang, Kang-Ning Shen
Sepioteuthis lessoniana is a crucial squid species in fisheries and plays a critical role in marine ecosystems. At least two taxa of the squid species have been identified in the waters around Japan; however, the basic biological information of these taxa is limited. In this study, the taxa composition of Sepioteuthis was examined using molecular evidence, and the life-history traits and statolith
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Gillnet selectivity for swimming crab Portunus trituberculatus in the East China Sea Fish. Sci. (IF 1.173) Pub Date : 2021-01-09 Guoqiang Xu, Wenbin Zhu, Liuxiong Xu
To explore the gillnet selectivity for swimming crab Portunus trituberculatus, six mesh sizes (100, 110, 120, 130, 140 and 150 mm) were tested to determine the optimal mesh size for swimming crab. The experiments were conducted in the traditional area for fishing crab in the East China Sea from 25 to 29 July 2018. Four kinds of selectivity curves were used for fitting, and the maximum likelihood method
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Interannual variability of larval fish assemblages associated with water masses in winter in the Taiwan Strait during 2007–2013 Fish. Sci. (IF 1.173) Pub Date : 2021-01-08 Yu-Kai Chen, Chia-Yi Pan, Yi-Chen Wang, Hsiu-Ju Tseng, Bo-Kun Su, Ming-An Lee
In this study, the interannual variations of ichthyoplankton assemblages in the Taiwan Strait (TS) during the winters of 2007–2013 were determined. The cold China Coastal Current (CCC) and Mixed China Coastal Water (MCCW) intruded into the TS and impinged with the warm Kuroshio Branch Current (KBC) with annual variations. Consequently, the ichthyoplankton community in the TS was mainly structured into
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Development of biosensor for measuring oxidative stress of fish Fish. Sci. (IF 1.173) Pub Date : 2021-01-06 Haiyun Wu, Madoka Ogata, Hitoshi Ohnuki, Hideaki Endo
To elucidate the dynamics of oxidative stress in fish, it is necessary to know the concentration of superoxide anions as a precursor to various reactive oxygen species in the living body. Superoxides are unstable radicals, disappearing within a few seconds, which makes it challenging to measure them using conventional methods. We describe herein the development of a biosensor that can rapidly quantify
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Suppression of male reproductive activity by estradiol in goldfish: appearance of infertile males that perform active spawning behavior Fish. Sci. (IF 1.173) Pub Date : 2021-01-06 Makito Kobayashi, Mai Kijima, Yuiko Matsuzuka, Youichi Hayakawa, Eri Iwata, Takeji Kimura
Estrogenic endocrine-disrupting chemicals are an increasing social concern, as they can reduce the reproductive activity of wildlife. The present study aimed to clarify the long-term effects of estradiol-17β (E2) on male reproductive activity in goldfish. Sexually mature male goldfish were implanted with a silicone capsule containing E2, and kept for 14 weeks or 15 weeks. The reproductive activity
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Establishment of a tracing technique for transplanted bluefin tuna germ cells in recipient’s gonads using monoclonal antibodies specifically recognizing bluefin tuna spermatogenic cells Fish. Sci. (IF 1.173) Pub Date : 2021-01-06 Ryosuke Yazawa, Tsubasa Kubokawa, Kensuke Ichida, Wataru Kawamura, Reoto Tani, Shigeharu Kamio, Tetsuro Morita, Goro Yoshizaki
Our ultimate aim is to establish a small-bodied surrogate broodstock (e.g., mackerel) that produces functional gametes of the Pacific bluefin tuna Thunnus orientalis using xenogeneic germ cell transplantation. To accomplish this goal, we need to be able to sequentially trace transplanted donor cells in recipients. Here we succeeded in identifying two monoclonal antibodies that could specifically recognize
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Hemolytic compound 3,7,11,15-tetramethyl-hexadecan-1,19-disulfate found in the invasive European sea squirt Ascidiella aspersa Fish. Sci. (IF 1.173) Pub Date : 2021-01-05 Hiroshi Nagai, Shuya Shibahara, Ryoji Matsushima, Hajime Uchida, Makoto Kanamori, Yasuyuki Nogata, Michiya Kamio
The solitary European sea squirt Ascidiella aspersa was introduced to northern Japan, causing dense coverage of the aquacultured Japanese scallop Mizuhopecten yessoensis. There are concerns that allelopathy by the sea squirt will suppress the growth of the scallop. Herein, we studied the allelopathic and defensive potential of A. aspersa using hemolytic assay. An alkyl sulfate, viz. 3,7,11,15-tetramethyl-hexadecan-1
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Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo Fish. Sci. (IF 1.173) Pub Date : 2021-01-04 Mujahid Ali Shah, Taiju Saito, Radek Šindelka, Viktoriia Iegorova, Marek Rodina, Abdul Rasheed Baloch, Roman Franěk, Tomáš Tichopád, Martin Pšenička
The cleavage pattern of a vertebrate’s embryo is either holoblastic (complete) or meroblastic (partial). Sturgeon and other basal bony fishes represent a transition of the cleavage pattern. To understand the transition, it is essential to develop an effective technique for the inhibition of specific blastomere cleavage. So far, various studies have demonstrated that diatom-derived polyunsaturated aldehyde
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An evaluation of the growth-promoting effects of green light on spotted halibut for its practical application in aquaculture Fish. Sci. (IF 1.173) Pub Date : 2021-01-04 Daisuke Shimizu, Kanta Mizusawa, Tomoki Maeda, Daigo Yamaguchi, Akiyoshi Takahashi
We examined the effects of tank color and stocking density on the growth of spotted halibut (Verasper variegatus) under a green light-emitting diode (LED). Fish reared for 40 days under a green LED (10 μmol m–2 s–1 at the water surface) grew faster than those reared under a white LED, irrespective of tank color (white or black). Green light promoted higher growth than indoor room light when fish were
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Fish cytokines: current research and applications Fish. Sci. (IF 1.173) Pub Date : 2020-12-03 Masahiro Sakai, Jun-ichi Hikima, Tomoya Kono
Cytokines are low molecular weight glycoproteins involved in the regulation of the immune system, and more than 100 cytokines have hitherto been identified in humans. Cytokines similar to those found in humans have also been found in fish. The innate immune response of fish can be examined by using cytokines as markers. We developed a multiplex reverse transcription–polymerase chain reaction assay
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The mysterious feeding ecology of leptocephali: a unique strategy of consuming marine snow materials Fish. Sci. (IF 1.173) Pub Date : 2020-11-25 Katsumi Tsukamoto, Michael J. Miller
Leptocephalus larvae have transparent bodies with tubular intestines that usually lack identifiable food items when they are collected, so mystery has surrounded efforts to determine what they feed on. Artificially spawned and reared first-feeding larvae were found to be highly selective in what they would eat, but they would consume rotifers and eventually ate specially formulated diets that contained
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The influence of sex and season on some hematological and biochemical parameters of snow trout Schizothorax labiatus in the Indian Himalayan Region Fish. Sci. (IF 1.173) Pub Date : 2020-11-21 Kousar Jan, Imtiaz Ahmed
Snow trout Schizothorax labiatus constitute a significant part of the diet of large human population of colder regions of the Indian Himalayan subcontinent, and are also important for local livelihoods there. In the present study, the hematological and serum biochemical parameters of S. labiatus were examined with respect to sex and season. Hemoglobin (Hb) concentration, red blood cell (RBC) count
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Touch-sensitive bristles on the carapace of the mud crab Scylla paramamosain may be receptors for courtship signals Fish. Sci. (IF 1.173) Pub Date : 2020-11-17 Gunzo Kawamura, Teodora Uy Bagarinao, Chi Keong Loke, Hsein-Loong Au, Annita Seok Kian Yong, Leong-Seng Lim
Bristle patches on the carapace behind the eyes were found for the first time in both males and females of the four species of mud crab Scylla spp. Electrocardiography revealed that the bristle patches of Scylla paramamosain were sensitive to touch (with a glass capillary) but not to chemical stimulation (with sugarcane juice). To elucidate the function of these touch signals, the courtship behaviour
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Cortisol induces masculinization of XX medaka through gonadal soma-derived growth factor (GSDF) and anti-Müllerian hormone receptor type 2 (AMHR2) Fish. Sci. (IF 1.173) Pub Date : 2020-11-17 Seiji Hara, Rie Sawamura, Takeshi Kitano
The medaka Oryzias latipes is a teleost fish with an XX/XY sex determination system similar to that of mammals. However, under high-temperature conditions, XX medaka are masculinized by an elevation of the key teleost glucocorticoid, cortisol. Cortisol inhibits female-type proliferation of germ cells and induces masculinization of XX medaka during gonadal sex differentiation. To identify masculinization
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Multiple cocaine- and amphetamine-regulated transcript genes in yellowtail Seriola quinqueradiata : cloning, tissue distribution in the brain, and response to fasting and fish meal soluble fraction Fish. Sci. (IF 1.173) Pub Date : 2020-11-10 Haruhisa Fukada, Koji Murashita, Ayaka Senzui, Toshiro Masumoto
Cocaine- and amphetamine-regulated transcript (CART) is an anorexigenic peptide hormone of fish and other vertebrates. In aquaculture, appetite regulation of fish is important to improve their growth. We cloned five CART genes (cart1b, 2a, 2b, 3a, and 3b) from yellowtail Seriola quinqueradiata, which belong to CART groups 1, 2, and 3 across four CART branches according to phylogenetic analysis. The
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The influence and impact of tsunamis on the microorganism assembly of Nagatsura-Ura Lagoon, Miyagi, northeastern Japan Fish. Sci. (IF 1.173) Pub Date : 2020-11-07 Yutaka Okumura, Hiromi Matsuoka, Hisayuki Arakawa, Fuyuki Tokanai, Atsushi Suzuki, Toshiaki Irizuki, Hiroto Kajita, Motoyuki Hara
Two sediment cores were collected from two sites in Nagatsura-Ura Lagoon, the mouth of which was destroyed in the 2011 Tohoku-oki tsunami. Although sediment conditions differed widely between the central and inner parts of the lagoon, we could identify traces of the 2011 Tohoku-oki tsunami at both, and those of earlier tsunamis in the inner part of the lagoon. Chlorophyll a maxima were observed at
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Effect of cold-anesthetization rate on blood biochemical parameters and muscle composition during live channel catfish Ictalurus punctatus waterless preservation Fish. Sci. (IF 1.173) Pub Date : 2020-11-05 Chan Bai, Guangquan Xiong, Ping Xu, Ning Li, Juguang Wang, Tao Liao
Cold-anesthetization waterless live fish preservation is considered a promising alternative strategy. This work investigates the effects of temperature chilling rates of 2, 4, and 6 °C/h on serum biological parameters and muscular chemical composition of channel catfish Ictalurus punctatus and the influence of waterless preservation time (6, 9, 12, 15, and 18 h) on survival after subjection to three
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Bycatch and discards from two types of bivalve dredges targeting Donax trunculus and Chamelea gallina used in the southern coast of the Marmara Sea, Turkey Fish. Sci. (IF 1.173) Pub Date : 2020-11-04 Serhat Çolakoğlu
Intensive commercial harvesting of the wedge clam Donax trunculus and the striped venus Chamelea gallina was conducted along the southern coast of the Marmara Sea using a mechanical dredge (MD) and a hand dredge (HD). The purpose of this study was to characterize and quantify the bycatch and discards of both types of dredge. The dredge tows were performed along sandy bottoms at depths ranging from
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Tissue distribution of tetrodotoxin and its analogs in Lagocephalus pufferfish collected in Vietnam Fish. Sci. (IF 1.173) Pub Date : 2020-10-28 Linh Vu Thuy, Shigeru Yamamoto, Rika Kawaura, Naoki Takemura, Kohei Yamaki, Ko Yasumoto, Kentaro Takada, Shugo Watabe, Shigeru Sato
Pufferfish belonging to Lagocephalus are composed of several species, some of which have been recognized to be non-toxic. Although fish belonging to this genus inhabit a wide variety of seawaters from temperate to tropical, toxin distribution has remained unclear. The present study was conducted to thoroughly survey the presence of tetrodotoxin (TTX) and its analogs (TTXs) in the extracts from various
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Growth and maturation of three commercially important coral reef species from American Samoa Fish. Sci. (IF 1.173) Pub Date : 2020-10-23 Cassandra Pardee, Brett M. Taylor, Sean Felise, Domingo Ochavillo, Javier Cuetos-Bueno
This study presents age-based life history information on three small-bodied species targeted in the American Samoan fishery: Chlorurus japanensis palecheek parrotfish/fuga-alosama, Lethrinus rubrioperculatus spotcheek emperor/fiola pa`o`omumu, and Naso lituratus orangespine unicornfish/ili`ilia. Age and reproductive information were derived from sagittal otoliths and gonads. Maximum observed ages
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The distribution and early growth of juvenile Pacific bluefin tuna Thunnus orientalis around Sado Island in the eastern Sea of Japan Fish. Sci. (IF 1.173) Pub Date : 2020-10-18 Hiroshige Tanaka, Taketoshi Kodama, Nobuaki Suzuki, Yosuke Mochizuki, Hiroshi Ashida, Takuya Sato, Hirohiko Takeshima, Kenji Nohara
The distribution and early growth of juvenile Pacific bluefin tuna (PBF) Thunnus orientalis were studied based on trawl surveys conducted around Sado Island, eastern Sea of Japan, from late August to early September in 2017 and 2018. Juvenile PBF were mainly collected in an area between the Tsushima Warm Current and land where the surface water (5-m depth) temperature ranged from 22.8 to 27.2 °C. The
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The effect of fish freshness on myosin denaturation in flounder Paralichthys olivaceus muscle during frozen storage Fish. Sci. (IF 1.173) Pub Date : 2020-10-04 Xinru Fan, Kunihiko Konno, Xiaoyu Lin, Xilaing Yu, Yuxuan Liu, Xiuping Dong
The effect of fish freshness on myosin denaturation during the frozen storage of flounder muscle was studied. The muscle of instantaneously killed flounder was immediately stored at − 20 °C (pre-rigor mortis; day 0), or after storage at 0 °C for 2 days (rigor mortis) or 5 days (post-rigor mortis). Myosin denaturation in frozen stored muscle was studied by measuring Ca2+-ATPase activity, myosin solubility
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Variations in female swordtip squid Uroteuthis edulis life history traits between southern Japan and northern Taiwan (Northwestern Pacific) Fish. Sci. (IF 1.173) Pub Date : 2020-10-01 Yumeng Pang, Chih-Shin Chen, Yoko Iwata
Swordtip squid Uroteuthis edulis is a commercially important species in coastal waters throughout the Northwestern Pacific, including those of Japan and Taiwan. Despite the importance of U. edulis to fisheries, previous studies on the life history traits (growth rate, size at maturity, and fecundity) of its populations in the Northwestern Pacific have been localized and region specific. Thus, we report
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Seasonal variations in phytoplankton productivity in a shallow cove in the eastern Seto Inland Sea, Japan Fish. Sci. (IF 1.173) Pub Date : 2020-10-01 Hitomi Yamaguchi, Nozomi Koga, Kazuhiko Ichimi, Kuninao Tada
We examined phytoplankton productivity in Shido Bay, an active oyster farming area in the eastern Seto Inland Sea, Japan, by an in situ 13C method from March 2016 to January 2017. The depth-integrated daily phytoplankton production ranged from 0.13 to 1.61 g C m−2 day−1, and the estimated annual production was 218 g C m−2 year−1. The daily production peaked in the rainy season and autumn, when phytoplankton
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Involvement of oxygenase confers higher resistance to neonicotinoid insecticides in estuarine resident sand shrimp Crangon uritai than in kuruma prawn Penaeus japonicus and mysid Americamysis bahia Fish. Sci. (IF 1.173) Pub Date : 2020-09-30 Takeshi Hano, Katsutoshi Ito, Nobuyuki Ohkubo, Mana Ito, Akio Watanabe, Hideo Sakaji
Globally, neonicotinoid contamination in aquatic environments, including estuarine areas, is a prevailing environmental concern. The estuarine resident marine crustacean sand shrimp Crangon uritai was previously found to have a higher tolerance to neonicotinoids than the marine crustaceans kuruma prawn Penaeus japonicus and mysid Americamysis bahia. Based on these findings, we aimed to explore the
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Starvation–refeeding causes cellular stress responses in the gut and liver of Masu salmon Oncorhynchus masou masou Fish. Sci. (IF 1.173) Pub Date : 2020-09-29 Hidehiro Kondo, Hiroaki Sano, Yuanyuan Wang, Junya Kawase, Eiji Shimanoki, Walissara Jirapongpairoj, Reiko Nozaki, Ikuo Hirono
Gene expression profiles during the transition from fasting to refeeding were investigated in the gut and liver of Masu salmon Oncorhynchus masou masou. Fish were starved for 3 days and then fed at 3% of body weight. The gut and liver were resected before and after feeding, and the tissues were used for transcriptome analyses. Twenty-nine and 45 genes were more than eightfold differently expressed
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Salinization intensifies the effects of elevated temperatures on Channa striata , a common tropical freshwater aquaculture fish in the Mekong Delta, Vietnam Fish. Sci. (IF 1.173) Pub Date : 2020-09-20 Tran Thi Phuong Lan, Tran Thi Thanh Hien, Tran Le Cam Tu, Nguyen Van Khanh, Yutaka Haga, Tran Minh Phu
Increasing water temperatures and salinity intrusion due to climate change are serious challenges for freshwater aquaculture. In this study, we assessed the combined effects of salinization at 0, 6, and 9‰ and water temperatures of 28, 31, and 34 °C on the survival and growth rates, and feed utilization, of freshwater snakehead Channa striata. We also assessed feed digestibility in snakeheads to explore
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Simulation of the capture process in set net fishing using a fish-schooling behavior model Fish. Sci. (IF 1.173) Pub Date : 2020-09-19 Yuki Takahashi, Kazuyoshi Komeyama
Generally, the catch efficiency (CE) of a set net is difficult to evaluate because it is affected by fish behavior. Therefore, to evaluate CE, we proposed a fish school behavior simulation model based on the boid model and applied it to a full-scale set net. The moving path of the fish school around the set net was simulated and visualized via the proposed method. From the moving path, three behaviors
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MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples Fish. Sci. (IF 1.173) Pub Date : 2020-09-15 Masaki Miya, Ryo O. Gotoh, Tetsuya Sado
We reviewed the current methodology and practices of the DNA metabarcoding approach using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA (approx. 170 bp from the mitochondrial 12S rRNA gene) across a wide variety of taxa. This method has mostly been applied to biodiversity monitoring using environmental DNA (eDNA) shed from fish and, coupled with next-generation
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Evaluation of hemolymph biochemical properties, clearance rate, bacterial microbiota and expression of HSP genes of gulf pearl oyster Pinctada radiata in response to salinity changes Fish. Sci. (IF 1.173) Pub Date : 2020-09-04 Saeid Tamadoni Jahromi, Sajjad Pourmozaffar, Hossein Rameshi, Mohsen Gozari, Reza Nahavandi
Hypo-salinity exposure is a serious concern for bivalve aquaculture, with profound effects on the animals’ physiological condition. The present study aimed to evaluate the influence of low-salinity exposure on the clearance rate, total heterotrophic marine bacteria and total Vibrio count in flesh, as well as the biochemical parameters of hemolymph and heat shock protein (HSP) gene expression, in Pinctada
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Seasonal variations in major components of Crassostrea gigas from Seto Inland Sea Fish. Sci. (IF 1.173) Pub Date : 2020-08-28 Chiaki Isono, Hitomi Maruta, Yun Ma, Natsuki Ganeko, Tsuyoshi Miyake, Hiromi Yamashita
The proximate composition and nutritional content of oysters define their quality and commercial value. Cultivation site and harvest date may impact these characteristics. We assessed moisture, ash, protein, fat, and glycogen concentrations, mineral and free amino acid content, and free fatty acid composition in oysters Crassostrea gigas from three coastal regions of the Seto Inland Sea, Hiroshima
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An economic evaluation of recreational fishing in Tango Bay, Japan Fish. Sci. (IF 1.173) Pub Date : 2020-08-25 Yuki Terashima, Yoh Yamashita, Kota Asano
Although recreational fishing is popular in Japan, few estimates have been made of its economic value to the country. This study aims to (1) evaluate the economic value of recreational fishing in Tango Bay, Kyoto Prefecture using the travel cost method (TCM); and (2) explore the development of related regional economies around Tango Bay. The TCM analysis showed that 150,000 anglers visit Tango Bay
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Feeding habits of the cyprinid Gymnocypris firmispinatus in the Anning River, China Fish. Sci. (IF 1.173) Pub Date : 2020-08-19 Baoshan Ma, Bin Xu, Kaijin Wei, Xiangyun Zhu, Jin Xu, Jianchao Lu, Jun Wang
The feeding habits and prey selection of Gymnocypris firmispinatus in the Anning River were investigated with respect to fish size, season and sex. Gut contents of 305 individuals ranging in size from 57 to 193 mm total length were analyzed, and 16.0% of the guts were found to be empty. The vacuity index indicated that the feeding intensity of the fish roughly followed a seasonal trend, with minimum
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Sea-entry conditions of juvenile chum salmon Oncorhynchus keta that improve post-sea-entry survival: a case study of the 2012 brood-year stock released from the Kushiro River, eastern Hokkaido, Japan Fish. Sci. (IF 1.173) Pub Date : 2020-08-18 Kentaro Honda, Kotaro Shirai, Shinji Komatsu, Toshihiko Saito
We examined the sea-entry conditions (i.e., timing and body size) of juvenile chum salmon that improve their survival during their coastal residency. On 25 June 2013, we sampled 365 juvenile chum salmon [57.5–98.6 mm fork length (FL)] off Konbumori, eastern Hokkaido, ⁓ 20 km east of the Kushiro River mouth, which originated from a hatchery in the Kushiro River. Sea-entry conditions of these Konbumori
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Evaluation of the properties of tuna skin gelatin as a hard capsule material Fish. Sci. (IF 1.173) Pub Date : 2020-08-18 Mala Nurilmala, Sendy Chrisman Adinugraha, Agoes Mardiono Jacoeb, Susi Susilawati, Yoshihiro Ochiai
Gelatin, which is thermally converted from collagen, is widely utilized in the pharmaceutical industry as a raw material for hard and soft capsules. The aim of this study was to produce gelatin from yellowfin tuna skin, the by-product of tuna processing, and determine the optimal concentration for hard capsule production. Capsules were prepared from the skin gelatin. The tuna skin gelatin was characterized
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High-resolution chemical profiling and antiparasitic potential of the tropical shrub Dillenia suffruticosa Fish. Sci. (IF 1.173) Pub Date : 2020-08-15 Muhammad Dawood Shah, Kazuki Tani, Balu Alagar Venmathi Maran, Yoong Soon Yong, Ching Fui Fui, Sitti Raehanah Muhamad Shaleh, Charles S. Vairappan
The aquaculture industry is growing rapidly throughout the world, but due to intensification, fish hatcheries are often faced with infestations of parasites, which can lead to economic loss. Among these parasites, the leech Zeylanicobdella arugamensis (Hirudinea: Piscicolidae) has been reported to impact hybrid groupers and other hosts. The objective of this study was to test the antiparasitic potential
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Sexual bipotentiality of the olfactory pathway for sexual behavior in goldfish Fish. Sci. (IF 1.173) Pub Date : 2020-08-12 Yu Shinohara, Makito Kobayashi
The goldfish Carassius auratus is a gonochoristic fish, but heterotypic sexual behavior can be induced by hormonal treatment in both males and females. Olfaction is essential for gender-typical sexual behavior in both male and female goldfish. Males have a stimulatory olfactory pathway for their sexual behavior. Female sex pheromones elicit male-typical sexual behavior in males. Olfactory blockage
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Primary structures and conformations of stonefish toxin-like toxins from three species of rabbitfish, Siganus puellus , Siganus unimaculatus , and Siganus virgatus Fish. Sci. (IF 1.173) Pub Date : 2020-08-12 Aya Yoshinaga-Kiriake, Yuji Nagashima, Shoichiro Ishizaki, Kazuo Shiomi
This study was undertaken on three species of rabbitfish, Siganus puellus, Siganus unimaculatus, and Siganus virgatus, to clarify whether stonefish toxin-like toxins are distributed in rabbitfish. The primary structures of toxins from these three species of rabbitfish were successfully elucidated by complementary DNA cloning and found to comprise two different subunits with almost the same molecular
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Cloning and tissue distribution of the ATP-binding cassette subfamily G member 2 gene in the marine pufferfish Takifugu rubripes Fish. Sci. (IF 1.173) Pub Date : 2020-08-09 Takuya Matsumoto, Saemi Kitajima, Chisato Yamamoto, Mitsuru Aoyagi, Yoshiharu Mitoma, Hiroyuki Harada, Yuji Nagashima
In this study, we cloned the ATP-binding cassette (ABC) subfamily G member 2 gene (Abcg2 gene; breast cancer resistance protein) from the liver of the marine pufferfish Takifugu rubripes, and examined its tissue distribution and gene structure. The full-length complementary DNA (cDNA) was 1977 base pairs (bp), comprising 15 exons in chromosome 17, and was assigned to the teleost Abcg2b paralogue. The
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Estimating biological half-lives of 137 Cs in a cyprinid fish Tribolodon hakonensis by a one-compartment model considering growth dilution effect Fish. Sci. (IF 1.173) Pub Date : 2020-08-03 Kouji Niizeki, Toshihiro Wada, Kenji Nanba, Keiichi Sasaki, Wataru Teramoto, Shigehiko Izumi, Hirotaka Nomura, Naohiko Inatomi
Japanese dace Tribolodon hakonensis rearing experiments were conducted to estimate parameters related to 137Cs uptake and excretion processes. Fish (initial mean total length 256 mm, 136 g) were reared at 20 °C for 182 days using the same daily ingestion rate (1% of body weight for 6 days per week) with three feeding regimens: feeding contaminated pellets (80.7 Bq/kg-dry of 137Cs) throughout the experiments
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The reproductive biology of the hermaphroditic goldlined seabream Rhabdosargus sarba in the central waters of the Taiwan Strait Fish. Sci. (IF 1.173) Pub Date : 2020-08-03 Shyh-Bin Wang, Tse-Lin Hsu, Shoou-Jeng Joung
The reproductive biology of a commercially important sparid, the goldlined seabream Rhabdosargus sarba, was determined from examination of 802 specimens (443 females, 232 males, 68 fish of undifferentiated sex, and 59 bisexual fish) collected by fisheries operating in the central waters of the Taiwan Strait (henceforth “central Taiwan Strait”) from January to December 2016. Previous studies undertaken
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Efficacy of commercial curing processes for treatment of quarantinable disease-causing pathogens in salmon roe Fish. Sci. (IF 1.173) Pub Date : 2020-08-03 Jun Nagata, Sayo Morimoto, Dominic K. Bagenda, Hisae Kasai
The distribution of salmon roe poses a risk to the salmon industry due to the potential transmission of quarantinable disease-causing pathogens. The curing of commercial salmon roe with saturated saline or soy sauce seasoning is widespread in Japan. The aim of this study was to evaluate the effect of commercial curing processes on quarantinable disease-causing pathogens [Aeromonas salmonicida, Renibacterium
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Effects of non-spawning season (yolkless to early yolk formation stage) temperature on growth and reproductive characteristics in hatchery-reared, repeat-spawning walleye pollock Gadus chalcogrammus Fish. Sci. (IF 1.173) Pub Date : 2020-07-31 Hiroshige Tanaka, Toru Nakagawa, Takashi Yokota, Masayuki Chimura, Yuuho Yamashita, Tetsuichiro Funamoto
The effects of water temperature during the non-spawning season (yolkless to early yolk formation stage of the ovary) on the growth and reproductive characteristics of 4-year-old hatchery-reared walleye pollock Gadus chalcogrammus (repeat spawners) were examined. Fish were kept in tanks under two temperature regimes (non-controlled: 6.4–17.2 °C; cooled: 5.1–15.7 °C) from 42 to 46 months post-hatching
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New sauces produced by koji fermentation of kamaboko show acceptable sensory characteristics, improved antioxidant activities, and high angiotensin-converting enzyme inhibition Fish. Sci. (IF 1.173) Pub Date : 2020-07-31 Yurika Nemoto, Yu Matsuo, Yumiko Yoshie-Stark
In our previous study, kamaboko was fermented in koji to prolong its shelf life and to increase its health-promoting effects. After fermentation, the texture of the kamaboko became cheese- or tofuyo-like. In the present study, sauces were produced by the fermentation of kamaboko in fermentation beds of wheat (WS), rice (RS), brown rice (BS), or soybean koji (SS), and their sensory qualities examined
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Determination of thermal tolerance in rainbow trout Oncorhynchus mykiss based on effective time, and its reproducibility for a large number of fish Fish. Sci. (IF 1.173) Pub Date : 2020-07-29 Toshinao Ineno, Kazuya Yamada, Koichi Tamaki, Ryusuke Kodama, Engkong Tan, Shigeharu Kinoshita, Koji Muto, Takashi Yada, Shoji Kitamura, Shuichi Asakawa, Shugo Watabe
Thermally selected male rainbow trout selectively bred at high temperatures were crossed with females of the Nikko strain with normal thermal tolerance. The F2 pedigrees, comprising 1641 individuals, were divided into 26 batches. The fish were acclimated to 17 °C, then their thermal tolerance determined by measuring the effective time (ET) required for them to lose equilibrium at an approximate upper
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Biochemical and physicochemical characteristics of the major muscle proteins from fish and shellfish Fish. Sci. (IF 1.173) Pub Date : 2020-07-21 Yoshihiro Ochiai, Hideo Ozawa
Fish and marine invertebrates constitute an important part of the human diet worldwide, and their muscles are the major edible parts. The muscles are a rich source of proteins, which in other terms determines the nutritional value and the quality of seafood products. Though the fish skeletal muscle proteins share many similarities with their mammalian counterparts, there exist great differences in
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Cholecystokinin 1 and 2 in red seabream Pagrus major : molecular cloning, response to feeding, and a potential indicator of dietary protein source quality Fish. Sci. (IF 1.173) Pub Date : 2020-07-21 Tran Thi Mai Huong, Koji Murashita, Ayaka Senzui, Toshiro Matsumoto, Haruhisa Fukada
Cholecystokinin (Cck) and peptide YY (Pyy) play important roles in digestive regulation in fish, but their function in red seabream (Pagrus major) remains obscure. Two cck complementary DNAs (cck-1 and cck-2) in red seabream were identified, and tissue distribution analysis of the genes revealed that cck and pyy were expressed throughout the digestive tract, except for stomach cck-2. After feeding
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Physiological changes in response to social isolation in male medaka fish Fish. Sci. (IF 1.173) Pub Date : 2020-07-17 Airi Otsuka, Moeko Inahata, Yuki Shimomura, Nao Kagawa
Fish that display schooling and social behaviors are expected to show stress responses when they are challenged by isolation. Medaka exhibit social behavior in group situations, but little is known about the effects of social isolation on their physiological stress responses. Here, we subjected male medaka to several different housing situations and assessed whether the stress responses differed between
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Bacterial and chemical properties of Japanese traditional anchovy product, salted Etari Fish. Sci. (IF 1.173) Pub Date : 2020-07-03 Takeshi Kobayashi, Mayu Nishitake, Masataka Saito, Takeshi Terahara, Chiaki Imada, Akira Shinagawa, Atsuko Takeshita
We analyzed the bacterial flora and chemical properties of the Japanese traditional anchovy product called salted Etari, which is distributed in Nagasaki Prefecture in the western part of Japan. The pH and NaCl concentrations ranged from 6.4 to 6.6 and from 9.6% to 13.9%, respectively. The lactic acid and total free amino acid concentrations were determined to be between 122 and 344 mg/100 g of sample
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Dietary supplementation with chitin hydrolysates for Anguilla japonica leptocephali Fish. Sci. (IF 1.173) Pub Date : 2020-07-01 Akihiro Okamura, Yoshiaki Yamada, Naomi Mikawa, Noriyuki Horie, Katsumi Tsukamoto
This study assessed the effects of diets supplemented with different types of chitin hydrolysate on larval growth of the Japanese eel Anguilla japonica. Larvae were reared for 26 days on artificial diets containing 2% of purified monomeric N-acetylglucosamine (NAG1), a mixture of mono-, di- and trimers (NAG3), a mixture of mono-, di-, tri-, tetra-, penta- and hexamers (NAG6), or a control diet. The
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Effects of astaxanthin on the immune response and reproduction of Procambarus clarkii stressed with microcystin-leucine-arginine Fish. Sci. (IF 1.173) Pub Date : 2020-06-30 Zhenhua An, Hui Yang, Xiaodan Liu, Yingying Zhangv
The aim of this study was to evaluate the effects of the antioxidant astaxanthin on the immune response, growth, and reproduction of the crayfish Procambarus clarkii (Girard, 1852) stressed with the toxin microcystin-leucine-arginine (MC-LR). P. clarkii exposed to MC-LR were treated with one of four concentrations of dietary astaxanthin supplement (0, 2.5, 5, or 10 mg/g) for 36 consecutive days in
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External factors that regulate movement in the marine rotifer Brachionus plicatilis Fish. Sci. (IF 1.173) Pub Date : 2020-06-25 Hee-Jin Kim, Masataka Ohtani, Akira Kakumu, Yoshitaka Sakakura, Atsushi Hagiwara
There are two types of movement pattern in the Brachionidae (rotifers), swimming and attachment, although the factors that induce a shift between them have not been adequately clarified. This study investigated the effects of five external factors—food limitation, temperature, salinity, predator, and un-ionized ammonia—on movement in females of the euryhaline rotifer Brachionus plicatilis sensu stricto
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Estimating the spawning season of black sea bream Acanthopagrus schlegelii in Hiroshima Bay, Japan, from temporal variation in egg density Fish. Sci. (IF 1.173) Pub Date : 2020-06-16 Kentaro Kawai, Hiroki Fujita, Gustavo Sanchez, Shuichi Furusawa, Tetsuya Umino
We aimed to determine the spawning period of black sea bream Acanthopagrus schlegelii in Hiroshima Bay, Japan, based on the distribution of its eggs, to assist in the resource management of this species. We developed a monoclonal antibody assay for the identification of black sea bream eggs, and appraised its specificity using immunohistochemical staining and DNA barcoding. To estimate the black sea
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Discrimination of nursery locations of juvenile Japanese flounder Paralichthys olivaceus on the Pacific coast of northern Japan based on carbon and nitrogen stable isotope ratios Fish. Sci. (IF 1.173) Pub Date : 2020-06-15 Yoshikazu Kato, Hiroyuki Togashi, Yutaka Kurita, Hiromitsu Kamauchi, Ichiro Tayasu
To test whether stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) can be used to understand the ecological connectivity of Japanese flounder Paralichthys olivaceus, we evaluated the isotopic signatures of juvenile P. olivaceus from the Pacific coast of northern Japan (i.e., from Aomori to Ibaraki Prefectures). We measured δ13C and δ15N in muscle and collagen extracted from vertebrae and found
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Fertility and gamete ploidy of allopentaploid offspring of tetraploid dojo loach Misgurnus anguillicaudatus females and large-scale loach Paramisgurnus dabryanus males Fish. Sci. (IF 1.173) Pub Date : 2020-06-12 Songqian Huang, Li Zhang, Xiaojuan Cao, Weimin Wang
Artificial interspecific hybrids (3n) of tetraploid dojo loach Misgurnus anguillicaudatus (TTTT) and diploid large-scale loach Paramisgurnus dabryanus (PP) were viable. Allopentaploid loaches (TTTTP) were produced by cold-shocking the fertilized eggs of TTTT females fertilized with sperm of PP males. The TTTTP were viable and well developed. As there are currently no reports on the fertility and gamete
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Molecular detection of protists associated with the Manila clam Ruditapes philippinarum inhabiting the Wajiro tidal flat in Hakata Bay, Fukuoka Prefecture Fish. Sci. (IF 1.173) Pub Date : 2020-06-12 Kiyotaka Takishita, Rihoko Kawai, Ayaka Tsutsumi, Goro Tanifuji, Mayuko Otsubo
Tidal flats are ecologically important as they support a large community of animals (e.g., crabs, mollusks, and polychaetes) and restore water quality. However, information regarding microbial eukaryotes, most of which are protists, associated with animals inhabiting these environments is limited. In the present study, utilizing polymerase chain reaction techniques, we detected fragments of the small
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Suitability of Japanese codling as a raw material for surimi-based products revealed by primary sequence analysis of myosin heavy chain and thermal gel properties Fish. Sci. (IF 1.173) Pub Date : 2020-06-08 Shugo Watabe, Daisuke Ikeda, Takaki Mashiro, Yuko Kagetakubo, Yoshihiro Takahashi, Misaki Uemura, Nanami Mizusawa, Hiroki Koyama, Ko Yasumoto, Mitsuru Jimbo, Nobuhiro Kan-no, Tomohiro Ueda, Yoko Matsuoka, Nobuhiko Ueki, Jianrong Wan
Complementary DNA encoding a part of myosin heavy chain was cloned from fast skeletal muscle of Japanese codling Physiculus japonicus. Japanese codling, a frequent catch off the Pacific coast of northeastern Japan, is a typical underutilized fish species, especially in summer. Phylogenetic analysis of the deduced amino acid sequence of Japanese codling myosin heavy chain loop 2 region revealed that
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Optimization of a spawning‑induction protocol for the prediction of natural coral spawning Fish. Sci. (IF 1.173) Pub Date : 2020-06-03 Go Suzuki
Many scleractinian corals only spawn once a year, so opportunities for collecting their gametes are limited. Consequently, the induction of spawning in coral would be useful, not only for studies on the mechanisms of coral reproduction, but also for stock enhancement. A spawning-induction method using hydrogen peroxide (H2O2) has already been developed for coral, but most adult corals died after exposure
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Larval identification based on melanophore patterns in two Auxis species, bullet tuna Auxis rochei and frigate tuna Auxis thazard , from the northwest Pacific Ocean Fish. Sci. (IF 1.173) Pub Date : 2020-06-02 Takuya Sato, Atsushi Tawa, Kay Sakuma, Masaki Sakurai
Larvae of Auxis rochei and Auxis thazard are commonly found in tropical to temperate waters around the world; however, their identification is difficult due to their similar morphological characteristics. In order to determine the morphological differences between larvae of these two species, we identified larvae of 192 A. rochei and 119 A. thazard using species-specific genetic markers and compared
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