For several years Tunisia has opted to breed freshwater fish in reservoirs and artificial lakes, created for irrigation, as a strategy of providing high quality aquatic protein to the interior regions and providing work opportunities for local communities. The aim of this study was to summarize the main results accumulated by the last 20 year efforts made by the members of the National Institute for

Classical cadherin gene family relies on calcium ions in playing an important role in adhesion between cells. However, comprehensive studies to identify classical cadherin gene families in Nile tilapia (Oreochromis niloticus) have not been conducted. In this study, 18 classical gene family members were screened from Nile tilapia using various bioinformatics methods. These screened cadherins were randomly

Lead, an environmental toxicant in our daily life, has been reported to have adverse effectson human and animals. Previous studies showed lead could accumulate in kidney tissues of carp and cause injury to the kidney tissues. In this study, we aimed to investigate the protective effects of andrographolide on lead-induced kidney injury in common carp and to clarify the possible mechanism. The results

In this study, we examined DNA sequences of bacteria isolated from goldfish intestine and identified the species in collaboration with the Food Industry Research & Development Institute of Taiwan. One bacterial species, Enterococcus avium, was selected for evaluation of its antimicrobial effects and probiotic function. The antimicrobial effects of feed containing E. avium were investigated in tilapia

To investigate the effect of dietary folic acid (FA) on growth performance, proximate composition, blood parameters, antioxidant status, and immune response of Siberian sturgeon, Acipenser baerii, six isonitrogenous and isolipidic experimental diets were formulated to contain graded levels of FA (0.60, 1.63, 2.71, 3.59, 4.82, and 5.68 mg kg−1). Each diet was fed to triplicate groups of fish (4.36 ± 0

Estradiol is a steroid hormone that regulates multiple physiological processes in teleosts. The present study aimed to investigate the role of 17β-estradiol (E2) in lipid metabolism in the liver of Nile tilapia. Waterborne exposures were performed using different concentrations of E2 (0 ng/L, 10 ng/L, 25 ng/L and 50 ng/L) and its receptor inhibitor ICI 182,780 (ICI) (1 μM/L). Subsequently, the survival

The cost of fish feed is a major constraint to fish farming in Sub-Sahara Africa. In the aquaculture value chain, feed is a determining factor and accounts for 60-75% of the total cost of fish production in many African countries. Therefore, 284 actors from all eight agro-ecological areas of Benin were interviewed and 28 local feed ingredients were collected as alternative ingredients for new fish

This study was aimed to evaluate the effects of dietary supplementation of probiotics Bacillus cereus (BC), B. subtilis (BS), Paracoccus marcusii (PM), and Lactobacillus plantarum (LP) on growth, immune response, antioxidant property, and intestine health of juvenile grass carp. Our results showed that dietary probiotics could promote the growth performance, which was significantly increased in BS

Mid1ip1 plays important roles in lipogenesis and microtubule stabilization, but little relevant information has been reported in fish. In the present study, mid1ip1 cDNA of tiger puffer was cloned and characterized, and its expression in response to dietary bile acid and lipid levels was investigated following a feeding trial. Experimental diets with suitable (8.5 %) or high lipid level (12.5 %), with

Rapana venosa is an economically important aquaculture species in China. Carboxypeptidases (CPs) A and B (CPA and CPB, respectively) hydrolyze peptides and proteins and play critical roles in the development of the digestive system in animals. In this study, a full-length CPA-like cDNA sequence from R. venosa (RvCPA) was isolated using RACE, and RvCPA mRNA expression levels were analyzed in different

A three-month feeding trial was conducted to investigate the effects of faba bean (Vicia faba L.) on growth performance, intramuscular collagen content, muscle cellularity and fillet texture of Yellow River carp, Cyprinus carpio haematopterus. Carps with an initial weight 500 ± 20 g were randomly divided into two treatments: commercial feed group (CFG) and faba bean group (FBG). Compared to CFG, the

Fish oil can not only provide energy for the growth and development of fish, but also constitute a variety of tissue and cellular structural components of fish. However, fish oil is easily oxidized in the process of feed processing, treatment and storage, which reduces the feed quality and threatens the health of fish. Therefore, the purpose of this study was to investigate the effects of different

Marennine is the blue-green pigment produced by the temperate ubiquiste marine diatom Haslea ostrearia. This pigment is water-soluble and excreted in seawater during H. ostrearia growth and ageing. Previous studies demonstrated the adverse effects of H. ostrearia culture supernatant that contains marennine (Blue Water, BW) against marine organisms. In tropical waters in the Southern hemisphere, a new

Probiotics and herbals are highly recommended in aquaculture; however, little is known about their combined effects. Therefore, the present study evaluated the possible synergistic impacts of Spirulina platensis (SP) and Bacillus amyloliquefaciens (BA) on the growth performance, histomorphology, and immune response of Nile tilapia (Oreochromis niloticus). A control and three test diets were enriched

This study was conducted to determine the minimum number of spermatozoa and optimal volume of activation water for effective fertilization of sterlet Acipenser ruthenus eggs. It was also to determine the minimum concentration of spermatozoa per ml of activation water during artificial propagation, when sperm with good motility and velocity (95 % motility and 150 μm s−1 curvilinear velocity) were used

As one of the key enzymes that maintain the dynamic balance of metal content in animals and take part in the process of heavy metals detoxification, metallothionein (MT) is also associated with the process of immune response. In this study, the full-length metallothionein cDNA sequence of 324 bp from Mytilus coruscus was obtained (named as McMT) with a 222bp ORF encoding 73 amino acid residues in length

Frass, a by-product of the larval meal industry, is heterogeneous and includes larval excrement, exoskeleton sheds and residual feed ingredients along with abundant nutrients, chitin and beneficial microbes. The present study was performed to evaluate the changes in growth, feed utilization, body composition, hematology, serum chemistry, immune responses and disease resistance of hybrid tilapia, Nile

The use of non-plant and plant-derived anesthetics in aquaculture activities is a frequent and necessary practice to maintain fish welfare. Currently anesthetics authorized for use in aquaculture activities are limited, and the use of unauthorized products is common. However, to obtain authorization for use in aquaculture and ensure their safety, anesthetics should be evaluated for their genotoxic

Pathogenesis and immune gene expression of the iridescent shark catfish Pangasianodon hypophthalmus to experimental Edwardsiella tarda infection was studied. Healthy Pangasianodon were inoculated intramuscularly with a sub-lethal dose of 1.77 × 107 cells/fish of E. tarda to induce edwardsiellosis. Petechial haemorrhages, inflammation at the site of injection, vertical hanging from the water surface

During the evolution of aquatic animals, co-evolution between host and microbes has been overlooked. Previous studies have shown that microbes were present in the fertilized egg of fish, but compared with fish, little information is available for crustaceans. High-throughput Illumina Miseq DNA sequencing of the V3-V4 domain of the 16S rRNA gene was used to assess the microbial community composition

This study investigated the influence of probiotic Pediococcus acidilactici MA 18/5 M on the metabolic enzyme activities and mRNA levels of growth, immunity, and antioxidative-related genes in zebrafish. Zebrafish fed with diets containing P. acidilactici MA 18/5 M at 0, 107, 108, and 109 CFU/g levels for eight weeks. The obtained results revealed that the creatine phosphokinase, lactate dehydrogenase

The winged pearl oyster (Pteria penguin) is one of the most potential species used to produce large sized pearls. Waterless transport and nuclei implant process are two common handling practices for pearl oyster in aquaculture. These processes can cause air exposure stress. In order to investigate the physiological energetics and oxidative stress of pearl oysters exposed to air, live P. penguin were

Six iso-nitrogenous and iso-lipid experimental diets were formulated to evaluate growth performance, intestinal digestive enzyme activity and the transcript expression of nutrition utilization related genes of the pacific white prawn, Litopenaeus vannamei (initial average body weight: 0.45 ± 0.01 g). The positive control group (LP) contains the 250.0 g/kg fish meal (FM) and 0 g/kg yeast hydrolysate

Members of lactic acid bacteria (LAB) have been well-known for probiotic agents owing to their ability to produce diverse beneficial compounds for cultured species, as well as for having a status of generally recognized as safe microorganisms. Considering these facts, the current study investigated the presence of cultivable LAB associated with the gastrointestinal tracts (GIT) of hybrid abalone (Haliotis

This study evaluated the sedimentation rates of nutrients and particulate matter in Nile tilapia (Oreochromis niloticus) net cage farming in the Umari, Brazilian semi-arid reservoir. Sedimentation chambers were installed under net cages which had stocking of 125 and 100 fish m−³ (experimental sites). The natural sedimentation rate of the reservoir (control site) was calculated using a sedimentation

The effects of starvation and re-feeding on Nile tilapia (Oreochromis niloticus) remain incompletely understood so far. In this study, the changes of intestinal microbiota, the metabolic and immune (antioxidant) responses of Nile tilapia (Oreochromis niloticus) during starvation and re-feeding stages were investigated. The metabolic responses (cortisol, ALT, AST, and leptin) and immune responses (hsp70

In the present study, the effects of β-glucan administration in water on growth performance and blood status (hematological indexes, immunological and biochemical parameters) were evaluated in Nile tilapia fingerlings (Oreochromis niloticus) under hypoxia-induced stress. In parallel, effect of this prebiotic (beta-glucan) was evaluated on gut microbiota diversity (16S rRNA gene) under normal oxygenation

The present study was conducted to replace fish meal with alternative plant protein sources in aquafeeds. Therefore, soybean meal (SBM), corn gluten meal (CGM), and mix of SBM + CGM were used as alternatives to fish meal (FM) with or without betaine (B) in Nile tilapia diets. Positive control diet using FM and six other diets were formulated to contain SBM, SBM + B, CGM, CGM + B, SBM + CGM, or SBM + CGM + B

This work aimed to determine fatty acid dynamics and lipid composition of female muscle, oocytes, newly hatched larvae (NHL) and first feeding larvae (FFL) of Prochilodus argenteus. Oocytes and NHL had the highest percentages of total lipids. Neutral lipids (NL) and polar lipids (PL) remained constant from oocytes through the larval stages (P < 0.05). Among NL, C18:2n 6 had its highest percentage in

Acute cold snaps induce a sudden reduction in water temperature and lead to high mortality of cultured Pacific white shrimp (Litopenaeus vannamei); however, little is known about the regulatory mechanisms in this shrimp species when adapting to acute cold stress. Mitogen-activated protein kinase (MAPK) signaling pathways are highly conserved and well known for their important roles in transducing environmental

We evaluated the growth and body traits of pacu and tambacu hybrid reared in cages and fed through different feeding programmes. Six treatments in a factorial scheme, with two genetic groups (pacu and tambacu) and three feeding programmes, were tested in 12 cages. In each cage, 50 juveniles (pacu, 120 ± 31 g; tambacu, 119 ± 28 g) were stocked and fed according to three feeding programmes using three

Peroxygen-based chemotherapeutants are commonly used in treating ectoparasitic infections in fish. A majority of relevant studies address the effects of these substances on the targeted causative agents, but little is known about their physiological impacts on the host organism. This study documented the changes in the skin of Atlantic salmon (Salmo salar) exposed to peracetic acid (PAA), a peroxygen

Bacillus spp. as probiotics have been widely used in the aquaculture industry. This study was designed to investigate the dietary supplementation of Bacillus cereus QSI-1 with quorum quenching activity, on intestinal barrier and expression levels of innate immune genes. After 14 d of feeding, fish fed with Bacillus cereus QSI-1 exhibited a significant down-regulated gene expression of TNF, IL-8, and

The growth performance and muscle quality of tilapia (Oreochromis niloticus) were compared between a fish-rice co-culture system (SD3) and a monoculture system (CG3). Tilapia with initial mean weight of 10 g and mean length of 5 cm raised in improved water quality produced by the co-culture system (SD3) showed better growth parameters, as well as better proximate composition with higher protein and

Probiotics can be used to reduce disease outbreaks in aquaculture. Some of them are characterised by their antagonistic activity against pathogens or the stimulation of the fish immune response, including the production of specific antibodies. If a probiotic has common antigens with a determined pathogen, it could produce antibodies with a cross-reaction to that pathogen. Thus, a probiotic with these

Mabé pearl culture is an increasingly important rural livelihood in south Pacific countries as it offers a low-cost, low-tech alternative to round pearl culture. Mabé pearl production can be achieved by local people with appropriate training, and the products offer further livelihood opportunities through value-adding and local production of jewellery and handicraft items. The Kingdom of Tonga is unique

The effects of Azomite, a natural mineral of volcanic ash, on the sex steroid hormone (SSH) levels and milt quality indices (MQI); namely, the milt volume, motility of spermatozoa, its duration and sperm count, were evaluated in tilapia (Oreochromis mossambicus). Several reports have documented the positive effect of the Azomite supplementation on growth, survival, and immune response of aquatic organisms

Crumbled feed (CF) is the most commonly used microdiet in the weaning phrase of large yellow croaker (Pseudosciaena crocea) in China. However, CF can be inefficient for local hatcheries due to its low water stability. In this study, we evaluated a more water-stable microdiet (micro-extrusion marumerization diet or MM) in comparison to the CF, and ascertained their effects on the growth of larval croaker

The genus Artemia (Crustacea; Anostraca) is a complex of sibling species and superspecies defined by the criterion of reproductive isolation. Two sexual species are represented in the New World: Artemia persimilis and Artemia franciscana. In Brazil, Artemia franciscana populations are found on a year-round and permanent basis in Rio Grande do Norte as a result of inoculations made in Macau in April

The effects of different linolenic acid (LNA)/linoleic acid (LA) on tissues fatty acid composition and steroid hormone secretion of the female yellow catfish Pelteobagrus fulvidraco were evaluated after an 8-week feeding trial. Four isonitrogenous and isolipidic formulated diets were designed to contain different LNA/LA ratios of D0.27, D0.82, D1.71 and D2.63, respectively. The result showed that there

Tambaqui Colossoma macropomum (CUVIER 1818) is a large Amazonian characid highly appreciated for its meat and therefore over explored by the fishing activity. For the last decades, tambaqui has been the first native fish produced by the Brazilian aquaculture industry. The Amazon Basin is characterized by the presence of acidic and basic waters with pH ranging from 4 to 7.2. In the wild, tambaqui migrates

A 10-week feeding trial was conducted to evaluate the effects of partially substituting soya protein concentrate (SPC), with white lupin (Lupinus albus) meal in carp (Cyprinus carpio) diets. This study further investigated the dietary inclusion of a solid-state fermentation (SSF) product of Aspergillus niger in tandem with SPC replacement. Six experimental diets were produced to be isonitrogenous (42%)

Pikeperch (Sander lucioperca) has a high potential for inland aquaculture diversification in Europe. Bottlenecks (i.e. low survival, cannibalism and deformity rates) hamper further expansion of pikeperch culture, because of the weak production performances at the nursery level. To improve the production of pikeperch juveniles under recirculation system we used a pilot scale larval rearing system (700

The yellow drum (Nibea albiflora; Na) constitutes an important seafood fish species that is subjected to intensive cage culture in China. The fish is susceptible to infection by bacteria and virtual pathogens, which results in considerable economic loss. In the present study, the pathogenic characteristics and immune response of the yellow drum were analyzed after Pseudomonas plecoglossicida or poly

Feed conversion ratio (FCR), the ratio between feed intake and body weight gain, is of major interest for improving aquaculture sustainability through reduced feed costs and environmental impacts. Demonstrating whether FCR measured in juvenile fish is an accurate predictor of their performance during the whole rearing period is critical to developing genetic improvement programs for this trait. This

The Chinese government has made tremendous efforts to promote the advancement of the aquaculture industry through the technical resources of its extension system. However, research on the Chinese aquaculture extension system is limited. The purpose of this study is to provide an overview of the role of China’s aquaculture extension system in the sustainable development of aquaculture since the Reform

The adoption of high standards of animal welfare improves the quality of life for animals being reared and produces improved end products for consumers. In this study, we investigate the role of environmental enrichment and tryptophan supplementation in increasing animal welfare indices in tilapia. To do this, the environment where fish are reared is enriched using artificial aquatic plants and shelters

Information on genetic variation in a population is useful to develop plans for sustainable management. Mitochondrial cytochrome b (Cyt b) gene sequence analysis has been found to be a powerful tool for assessing intraspecific genetic diversity of fish and other animals. We analyzed the partial sequences of the Cyt b gene of introduced and hatchery-bred Thai and Viet Nam P. hypophthalmus and indigenous

Lumpfish (Cyclopterus lumpus) are widely applied as biological delousers in open net-pen farming of Atlantic salmon. As a species new to farming it is necessary to obtain a comprehensive understanding of the capacity of lumpfish to utilize plant derived feed ingredients. A feeding trial lasting for 54 days was conducted to investigate the effects of replacing fishmeal (FM) with a mix of soy protein

The use of probiotics, prebiotics and synbiotics provides better growth rates, improves the innate immune system and protection against several bacterial pathogens. The species Aeromonas hydrophila causes high mortality rate and production loss, provoking an antimicrobial overuse. This study aimed to prove the capacity of commercial probiotic DBA® (Bifidobacterium sp, Lactobacillus acidophilus and

The presence of healthy gut microbiota in the gastrointestinal tract of fish is important for the optimal function of gut, which plays a significant role in the host growth and health. The aim of the present study was to investigate the effect of dietary stress worry free (SWF®) on growth, feed utilization and disease resistance of hybrid sturgeon (Acipenser baerii x Acipenser schrenckii). Sturgeon

Tambaqui (Colossoma macropomum) is the main native fish produced in continental aquaculture from South America. However, its production has been negatively affected by significant economic losses due to frequent outbreaks caused by the parasite Ichthyophthirius multifiliis. Genetic selection for I. multifiliis resistance may represent a sustainable and effective alternative to reduce mortality and

Cutthroat trout (Oncorhynchus clarkii) are widely cultured in state and federal hatcheries and private farms throughout the Western United States for stock supplementation or recreational fisheries. Cutthroat Trout culture techniques often rely on methods borrowed from the culture of closely related Rainbow Trout (Oncorhynchus mykiss). Even though commercial feeds for Rainbow Trout are commonly utilized

The present study analyzed the inputs, outputs, and accumulation of carbon in ecological compartments of earthen ponds for the grow-out of Amazon river prawn (Macrobrachium amazonicum) and tambaqui fish (Colossoma macropomum) reared in monoculture and integrated multi-trophic aquaculture (IMTA) systems, using recycled hypereutrophic water. A completely randomized experiment was designed with four treatments

An 8-week feeding experiment was conducted to investigate the effects of dietary n-3 highly unsaturated fatty acids (n-3 HUFAs) on growth performance, fatty acid profiles, hematological characteristics and hepatopancreatic histology in juvenile Pacific white shrimp (Litopenaeus vannamei) (initial average weight, 0.14 ± 0.01 g). Six isoproteic and isolipidic diets were formulated with graded levels

The use of lactic acid bacteria (LAB) is a strategy employed to improve aquatic animal health status and to prevent aquaculture diseases. In the present study, two potential LAB were isolated from the intestines of Channa argus (C. argus) towards the assessment of their probiotic potential. Based on the preliminary screening on 178 isolates obtained from the intestines of C. argus, only ten LAB isolates

A ten-week feeding trial on Monopterus albus (initial weight 24.02 ± 0.02 g) was conducted to evaluate effects of four isonitrogenous and isoenergetic diets in which different proportions of fish meal (0, 2.5 %, 5% and 7.5 %) were substituted with Hermetia illucens (HI) larvae meal, corresponding to dietary inclusion levels of 0 (FM), 5.26 % (HI5.26), 10.52 % (HI10.52) and 15.78 % (HI15.78). Compared

Aquaculture is a fast-growing sector with a high scope for augmentation. Microalgae are frequently used for fish larvae culture. Hatcheries face many problems regarding the maintenance of a stock culture of the microalgae. Long term maintenance of microalgal species by serial sub culturing is often expensive, time-consuming and leads to genetic change. The present study is aimed to develop an alternative

Protein hydrolysates from poultry by-product (CPH) and poultry by-product plus swine liver (PHPPL) were evaluated for digestibility and attractiveness for Litopenaeus vannamei, their effect on growth as a substitute for salmon by-product meal. Initially, the apparent digestibility coefficient (ADC) of the protein hydrolysates was determined and their attractiveness was also evaluated. Subsequently