The increase in overfishing and pollution in Pátzcuaro Lake, Michoacán, México, has generated a severe problem in the endemic species that live there; especially the Chirostoma estor estor that has been threatened. A computational model has been developed for the evaluation of water quality in freshwater intensive farming tanks to contribute to the conservation efforts of this species. The proposed

Piwi is necessary for germ cell development in a diverse range of organisms and homologues have been commonly used to identify primordial germ cells (PGCs) during embryogenesis. Here, we isolated full-length cDNA of Piwi ortholog in the Pacific oyster Crassostrea gigas and characterized its expression patterns (Cg-Piwi-like) along with analyzing the expression alternation in the gonad of diploids compared

This study analysed the effect of feeding a freshwater fish, barbel (Barbus barbus), with Artemia sp. enriched with polyunsaturated fatty acids (PUFA). Barbel larvae were divided into two groups, which received a daily dose of nauplii at 50 % of the fish body weight. One of the groups received nauplii enriched with PUFAs (group E), whereas the other received nauplii without enrichment and was used

Aquaponics is the integrated production of aquatic organisms and plants. The majority (>50 %) of nutrients sustaining optimal plant growth in aquaponics is derived from waste originating from the feeding of aquatic organisms kept in aquaculture. Currently, aquaponics is more popular as a hobby rather than for commercial-scale production. We used long-term data verification to present a qualitative

Aquaculture effluent is often associated with increased organic carbon, suspended solids, phosphates, nitrogenous species (nitrates, nitrites, and ammonia), chemical oxygen demand and biological oxygen demand. This is regarded as a global threat to aquatic ecosystems due to its influence on surrounding waters as well as groundwater. The threat of aquaculture effluent is not confined to the aquatic

This study investigated the effects of rapeseed meal (RM) as fish meal (FM) alternative on the growth rate, feed utilization, protein metabolism-related parameters, and antioxidant indices of Asian red-tailed catfish (Hemibagrus wyckioides). Five isoproteic and isocaloric diets were formulated with 0%, 11.2 %, 22.4 %, 33.6 %, and 44.8 % RM replacing graded levels of FM, respectively. Each experimental

This study provided a preliminary framework for the effects of Origanum vulgare L. essential oil (EO) on sea bass (Dicentrarchus labrax) health status over a 60-day feeding trial. Fish were fed twice a day until apparent satiety with three different diets: a control diet (CD), and two experimental diets supplemented with 100 (D100) and 200 (D200) ppm of oregano EO. No mortality was observed in each

A 60-day feeding trial was performed to test the nutritional value and utilization possibility of palm kernel meal (PKM) as a feed ingredient for juvenile Labeo rohita. Five iso-nitrogenous diets were prepared with various inclusions of PKM which replaced sunflower oil cake and de-oiled rice bran namely, control (without PKM), T1 (50 g/kg PKM), T2 (100 g/kg PKM), T3 (150 g/kg PKM) and T4 (200 g/kg

An experiment was carried out to evaluate the growth performance and yield of Labeo bata reared in cages at different stocking densities in a floodplain wetland of Assam, India. Fifteen cages (effective water volume 40 m3 each) installed in the wetland were stocked with fingerlings of L. bata (mean length 8.20 ± 0.08 cm; mean weight 4.82 ± 0.13 g) at five different stocking densities, i.e., 25 (D25)

At present, the application of probiotics in aquaculture is gaining wide acceptance for its beneficial effect on fish production. This study focused on evaluating the potency of a commercial multi-strain probiotic on mean growth, feed utilization parameters, yield and survival for profitable cage culture of striped catfish, Pangasianodon hypophthalmus at haor floodplain of Bangladesh. The present study

The optimal mixed feeding schedule for mono-sex male Nile tilapia (Oreochromis niloticus) was investigated in the current study. The fish (4.01 ± 0.02 g initial weight and 5.84 ± 0.04 cm initial length) were fed up to satiation with high protein (HP, 32.80 % protein on dry basis) and low protein (LP, 28.64 % protein on dry basis) diets in different schedules: alternately every other day (1HP/1 L P)

A feeding trial was conducted to investigate the effects of Chinese herbal medicines mixture (CHMM) on growth performance, digestive enzyme activity and serum biochemical parameters of European eels, Anguilla anguilla (initial weight 6.19±0.09 g). Diets with different supplementation of CHMM (0, 2.5, 5, 7.5, 10, 12.5 g/kg) were given to eels for 42 days. Results showed: eels fed with 5–12.5 g/kg CHMM

This study assessed the activity of the main digestive enzymes in juveniles of the painted river prawn Macrobrachium carcinus. Prawns reared in 24 m2 tanks at a density of 10 individuals. m−2 for 45 days were fed a commercial diet with 40 % crude protein. A sample of 21 prawns with 2.92 ± 0.60 g and 27.87 ± 1.50 mm had their midgut glands (MG) dissected. Assays prospecting total proteolytic activity

The study explored the effects of extract of mulberry leaves (EML) on the growth performance and hypoxia-reoxygenation stress as well as biochemical parameters in various organs of crucian carp (Carassius auratus gibelio). The results indicated that dietary EML improved the growth of crucian carp. The appropriate concentration of EML for fish growth was estimated to be 46.93 g kg−1 diet. Moreover,

Recently, the concept of precision nutrition has emerged in aquaculture, and there is increasing discussion on this topic. Some researchers have defined precision nutrition as precision feeding, which means that the nutrient contents of feed should meet the requirements for an animal’s optimal production performance and achieve minimum fodder waste at the same time. In practice, precision nutrition

The high nutritional quality of common sole Solea solea increases its value for the fishery industry and for the aquaculture sector. To ensure the expansion of its production, it is necessary to implement farming and broodstock management technique to produce high quality eggs and larvae. This work summarizes eight years of study on reproductive performances, growth and parental contribution of a common

An 84-day trial was performed to assess the use of alternative protein sources in Seriola dumerili. Three diets were used, FM100 diet, as a control diet without fishmeal substitution, and FM66 and FM33 diets with a fishmeal replacement of 330 g/kg and 660 g/kg, respectively. At the end of experiment, fish fed the FM66 diet showed the no differences in growth, nutritional parameters and fatty acid composition

We investigated whether six microsatellite (MS) markers previously developed for two marine crop species, Neopyropia yezoensis and Neopyropia tenera (formerly Pyropia yezoensis and Pyropia tenera, respectively), are useful for molecular analysis of gametophytic blades resulting from a cross between Neopyropia yezoensis and Neopyropia sp. 2, a cryptic species of the N. yezoensis complex. Three (Pye1

This study was conducted to identify the effects of organic acid supplementation on 50 % replacement of fishmeal by soybean meal on the growth performance, hepatic condition and intestinal histology of giant grouper Epinephelus lanceolatus. Giant grouper juveniles were fed three different diets, 50 % fishmeal protein replacement with soybean meal (SBM50), 50 % fishmeal protein replacement with soybean

In the present study, we analyzed and identified two CXC chemokine ligands in the swamp eel, Monopterus albus. The open reading frames of MaCXCL13 and MaCXCL14 were 375 bp and 300 bp, which encoded putative proteins of 124 aa and 99 aa, respectively. Phylogenetic and protein similarity analyses found that only MaCXCL14 has high homology with CXCL proteins from other vertebrates, while MaCXCL13 shared

An 8-week growth experiment was conducted to quantify the appropriate dietary choline requirement of juvenile Chinese sucker (Myxocyprinus asiaticus). Each of seven semi-purified diets containing choline at a level of 44.79 (basal), 164.95, 278.70, 574.29, 1076.52, 2124.29 or 4477.72 mg/kg diet was fed to three replicates with 30 fish (5.52 g per fish). The results showed that weight gain and specific

Seaweeds are known to contain nutrients that are essential for human nutrition, making them edible and potential resources to mine for bioactive substances. The present study was aimed to investigate the prebiotic potentials of the seaweed, Sargassum polycystum as supplement in diets for Asia sea bass fingerlings. Experimental dietary formulations were supplemented with four graded levels of powdered

Immunostimulants stimulate the immune system by involving many cytokines. Interferons, a crucial cytokine, shares both antiviral and antibacterial properties. Hence, the present study aimed to evaluate the effect of immunostimulant on type I interferon (I-IFNs) gene expression in Labeo bata. For the first time, genes coding for I-IFN has been cloned, amplified, and sequenced from L. bata (bata). The

Algae extracts or clay are supplement into animal feed to promote growth, digestive performance and health status. A feeding trial was conducted to evaluate supplemental effects of an Algae-clay powder (sea lettuce, Ulva lactuca and red algae, Solieria chordalis in exfoliated micronized montmorillonite, ACP) into a low fish meal (LFM) diet on growth and feed utilization, digestibility, innate immunity

This study investigated the interactive effects of mulberry leaf meals (MLM) and bamboo charcoal additive (BCA) on growth performance, anti-oxidant capacity, and disease resistance of genetically improved farmed tilapia (GIFT) juveniles (Oreochromis niloticus). An 8-week (56-day) feeding trial of 2 × 3 factorial experiment was carried out wherein fish were fed with six diets containing graded levels

A 60-day feeding trial was conducted to evaluate the effects of different substitution levels (0 %, 20 %, 40 %, 60 %, 80 % and 100 %, named as G1, G2, G3, G4, G5 and G6, respectively) of fishmeal with soybean meal on growth, feed utilization, intestinal microbiota composition and immunity of Carassius auratus gibelio ♀ × Cyprinus carpio ♂ (13.2 ± 0.2 g). Quadratic regression analysis of fish responses

Biofouling is one of the biggest challenges for mariculture in tropical waters. This study aims to evaluate different materials in control to biofouling in tropical waters through two experiments. For Experiment I, compared the accumulation of biofouling on different nylon panels and the use of antifouling (copper-base) paint that remained submerged for 8 weeks. In experiment II, evaluated multifilament

The present study aimed to investigate the growth, disease resistance and immune function of tilapia (Oreochromis niloticus) fed diets containing Enterococcus faecalis at levels of 0 (CG), 1 × 105 (EF1), 1 × 106 (EF2), 1 × 107 (EF3) or 1 × 108 CFU/g diet (EF4) for 56 days. The results showed that the EF3 group exhibited significantly increases in the final weight, weight gain and specific growth rate

A comparative study was performed to examine the effects of acute heat stress on non-specific immune response and heat shock protein 70 (hsp70) gene expression in three strains of koi (Cyprinus carpio), Showa Sanshoku, Kohaku, Ogon. Fish were exposed to increasing water temperature from 25 to 32 °C. Samples were collected at 0, 2, 6, 10, 14, 18, 22, 26 h after heat stress. Data were analysed by two-way

The biofloc culture system (BFT) allows for the production of aquatic animals at higher stocking densities compared to conventional aquaculture systems and it has been proven to be efficient for shrimp (Litopenaeus vannamei) and mullet (Mugil liza) rearing. Accumulation of nutrients enhances the natural productivity of the system, thus the concentration of total suspended solids (TSS) tends to accumulate

Larval development, especially settlement and metamorphosis, has a vital influence on commercial culture during the life cycle of marine invertebrates. Sipunculus nudus, a peanut worm, is an important economic aquaculture species. However, information about the mechanism of its larval settlement and metamorphosis is still limited. Transcriptomic analysis was performed to determine the DEGs between

Yam (Dioscorea oppositifolia L.), as an important traditional medicinal herb, has a functional impact upon anti-disease and strengthens immunity. In the present study, we conducted growth performance, serum biochemical level, metabonomics analysis of liver in rainbow trout (Oncorhynchus mykiss) fed diets including yam extract (0, 0.2 %, and 0.4 %) for 56 days, to identify yam extract-affected growth

This study aimed at determine the digestible lysine requirement of tambaqui juveniles using the diet dilution technique. A total of 250 juveniles tambaqui with an initial mean of 22.99 ± 1.06 g were used in a dose-response experiment with five levels of digestible lysine (0.865, 1.125, 1.383, 1.644 and 1.903%) and five replication per treatment. The digestible lysine levels evaluated did not influence

A bioeconomic model was used to assess the impact of acute hepatopancreatic necrosis disease on the economic performance of Litopenaeus (Penaeus) vannamei production. Seven disease severity scenarios were considered where the worst results were obtained when severity was extreme with a mean loss of US$ -727.56 ha−1, a benefit/cost ratio of 0.52, and a 95.9 % loss probability. The best results were

A 63-day feeding trial was conducted to explore dietary yeast cell wall (YCW) on growth, immunity and disease resistance against Aeromonas hydrophila in gibel carp (Carassius auratus gibelio var. CAS Ⅲ) (initial body weight: 8.49 ± 0.27 g). Four isonitrogenous and isoenergetic diets were formulated to contain 0 % or 0.4 % three types of yeast cell wall (YCW) (termed as YCW0, YCW1, YCW2 or YCW3, respectively)

The breakthrough in commercial shrimp breeding and farming in the 1960s has resulted in the establishment of shrimp aquaculture in Japan, Taiwan, China, The Philippines, Malaysia, Indonesia and consequently the other parts of the world. White-leg shrimp, Penaeus vannamei has become the main cultured shrimp species and accounted for 53% of the total crustacean aquaculture production. However, the intensifying

The hooded oyster, Saccostrea cuccullata (Born, 1778), is an important aquaculture species on the coast of Thailand. Its aquaculture is mainly based on wild seed collection, the availability of which is becoming unstable due to environmental changes and pollution. To support aquaculture of this species, seed production in the hatchery is therefore required. Unfortunately, information on seed production

The present study compared the nutrient compositions of peanut seed (Arachis hypogaea) subjected to three processing techniques; mechanical pressing, fermentation and germination. The study also evaluated the effect of inclusion level (0%, 15 %, 30 % and 60 %) on nutrient digestibility of these meals by juvenile barramundi (Lates calcarifer) and the subsequent discharge of nutrients to the rearing

Salinity intrusion in freshwater ecosystems of low-lying coastal areas poses a major threat to aquaculture and agricultural production. An experiment was conducted to observe the effects of salinity on early life development of the freshwater aquaculture species pabda, Ompok pabda. Fish embryos (n = 200) and fry (n = 100) were exposed to five different salinity concentrations viz., 0 (control), 2.5

This study sought to determine seasonal variations in wild fish assemblage events associated with fish farms with respect to fish biodiversity, abundance and biomass along the Turkish Aegean Sea. Assemblages of wild fish were determined bimonthly with six rapid visual counts (RVCs) at six fish farms in both the Izmir (Northern Aegean Sea, NAS) and Mugla (Southern Aegean Sea, SAS) regions. Data were

Light is an important environmental factor for the fitness of small sea urchins. It is thus important to reveal the appropriate light environments for their stock enhancement. However, the effects of light spectra on fitness related behaviors of sea urchins remain mostly unknown. To reveal appropriate light spectra for stock enhancement of the sea urchin Strongylocentrotus intermedius, we investigated

A 30-day feeding investigation was conducted to resolve the effects of starvation in juvenile loach (Paramisgurnus dabryanus). In this study, the digestive enzyme activity, antioxidant activity, immune enzyme activity, and microflora compositions were analyzed to investigate the effects of starvation. At the end of the 30-day trial, loaches in the feed and starved groups were sampled for enzyme activities

Recently, our preliminary research showed that 40–80 % of dietary fishmeal (FM, 30 %) could be successfully replaced by the terrestrial compound protein (CPro) in the carnivorous marine teleost, Trachinotus ovatus. Here, to further confirm the feasibility of CPro and evaluate their environmental benefits, another 8-week feeding trial was performed using the large size T. ovatus (initial body weight

Most fish species are susceptible to red-spotted grouper nervous necrosis virus (RGNNV) which causes viral encephalopathy and retinopathy leading to huge financial losses in the aquaculture farming industry for decades. A knowledge of RGNNV replication and the host response in depth is warranted to understand the host-virus interaction and to develop an effective approach to inhibit this virus. This

Despite being a tropical species, Nile tilapia is farmed in many subtropical regions worldwide, including southern Brazil, China, and Egypt, where production is hindered during the colder months of the year. This review aimed to identify and synthesize the main aspects that affect cold tolerance in Nile tilapia. There is sufficient evidence in the literature to show that modulation of dietary lipid

The relative contribution of natural feed sources and commercial diets to the nutrition of Nile tilapia (Oreochromis nilloticus) was evaluated in a net cage production carried out in a reservoir in the semi-arid northeast region of Brazil. The present study tested the hypothesis that tilapia uses natural sources of feed for growth, even when confined in cages and artificial feed resources are available

196 common carp (Cyprinus carpio L.) samples from five wild populations and ten domestic strains (six Chinese and four European) in China were collected and the genetic variation was analyzed using direct DNA sequencing of mitochondrial Cytochrome c oxidase subunit 2 gene (COII). As a result, a total of 22 haplotypes were identified, and one maternal ancestral haplotype (Hap_1) distributed in all populations

In the study, we evaluated the effects of dietary taurine supplementation on growth, tissue taurine concentrations, and nutrient digestibility in the giant grouper, Epinephelus lanceolatus, fed a diet containing soybean meal. The basal diet containing soybean meal (replacing 40 % of fish meal protein) was supplemented with taurine at 0 (control), 0.1, 0.3, 0.5, and 1 g/kg of the diet. An all-fish-meal

Since discovery, glucagon biology has remained enigmatic. Studies have shown that glucagon may have physiological effects beyond glucose regulation by binding to its receptor (Gcgr). The potential roles of glucagon and its receptor in the crosstalk between glucose metabolism and immune function of fish remain to be investigated. In the present study, we sequenced and characterized gcgra and gcgrb in

In teleosts, ghrelin plays an important role in body weight gain. This hormone has been used in functional studies on food intake and foraging-related behaviors. However, the regulatory role of its 5′ flanking and coding regions is unknown. To examine the effects of single nucleotide polymorphisms (SNPs) in the ghrelin gene of Nile tilapia, we cloned sequences of its 5′ flanking and coding regions

A 56-day feeding trial was performed to evaluate the effect of herbal-probiotic supplements comprising a mixture of Chinese herbs (CH) (i.e., Astragalus membranaceus, Angelica sinensis, and Crataegus hupehensis) and or commercially prepared probiotic Bacillus species (BS) (i.e., spores of B. subtilis and B.licheniformis) on the physiological response of Nile tilapia. Fish (50 ± 2 g) were fed six diets

The industrial space favors the creation of artificial water bodies which should be seen as a new resource of potential use. The aim of the study was to assess the possibility of using the newly created clarification pond from opencast mine dewatering system to extensive aquaculture and determine the effect of hydrochemical and hydrobiological parameters, i.e. water quality and trophic indices, on

In this study, we investigated rearing and breeding razor clam (Sinonovacula constricta) in inland alkaline brackish water. During 45 days of the experiment in candidate target inland area brackish water (TBW), survival, growth, enzyme activity and immune responses were analysed. The results showed that shell length and weight were significantly inhibited in the TBW group (p <0.001). Although the cumulative

Fouling assemblage colonizing fish-farms is considered mostly to produce negative impacts causing financial loss. By contrast, large evidences emerged on the bioremediation role by biofouling associated to aquaculture facilities, even if the fouling assemblages thriving in the water column was poorly investigated. The aim of the present work was to investigate the macrofouling assemblages over one

In trout aquaculture, tight control of water quality is crucial, especially for young life-history stages. This study examined whether chronic exposure to moderate hypoxia during the fry stage affects the rainbow trout’s behavior during later development. One month after yolk-sac resorption, young trout were exposed for 25 days to hypoxia, obtained by decreasing water flow and bubbling nitrogen gas

In the present study, we isolated bacteria from intestines of healthy rainbow trout, screened for their probiotic properties, and inhibitory activity against Y. ruckeri. A total of 21 out of 541 isolates from the intestine of adult rainbow trout (∼ 200–350 g), showed inhibition zones around at least one Y. ruckeri strains tested. Six of them were selected due to their ability to inhibit all the pathogenic

Infectious diseases usually occur in response to the highly intensive rearing conditions in fish farms. The use of feed additives is still one of the most practical options in the field of aquaculture. Therefore, this study aimed at evaluating the impact of raffinose on the growth rate, antioxidative status, and immunity of Nile tilapia. For 60 days, fish fed five test diets with raffinose at 0, 0

Ammonia, as one of the major environmental pollutants in aquaculture, can lead to negative impacts on health and production of farmed animals. However, the underlying molecular mechanism of ammonia in crayfish remains unclear. In this study, the transcriptomics of hemolymph, gill and hepatopancreas of Procambarus clarkii subjected to ammonia stress were analyzed through comparison with a control group