The potential effect of Salvadora persica dietary supplementation on Nile tilapia (Oreochromis niloticus) health was investigated. Four diets were formulated to contain 0 (control), 0.5, 1 and 2 % (w/w) S. persica powder. Fish were fed for eight weeks (pre-challenge period), then challenged with A. hydrophila for seven days (post-challenge period). Results showed that the immune and biochemical parameters

An earlier study showed that hybrid striped bass (Morone chrysops ♀ x M. saxatilis ♂) diets containing 20 % animal meals and up to 30 % aerobically fermented (AF) carinata meal (Brassica carinata, CM) followed by a single wash with water (AFCM) or 30 % CM double-washed with water without AF (WCM), resulted in similar growth. However feed utilization and Fulton’s condition factor of 30 % WCM were better

Tannins have great potential as an alternative to in-feed antibiotics. However, no study has investigated the effects of tannins on shrimp. The present study assessed the eff ;ects of hydrolyzable tannins (HTs) fed at different inclusion levels (F0: control, F0.5: 0.05 %, F1: 0.1 %, F1.5: 0.15 % and F2: 0.2 % (g−1 feed) on the growth performance, antioxidant capacity, immune response, intestinal microflora

A feeding trial was carried out to investigate the effects of dietary fermented rice protein (FRP) on growth, digestive and absorption capacities, inflammatory-related gene expression, and intestinal microbiota in juvenile hybrid groupers (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂). A fish meal (FM)-based diet was used as a control for comparison with diets in which FM was replaced by FRP

Sansha Bay, facing the East China Sea, is characterised by high fish production (Pseudosciaena crocea, 91,000 t yr–1) and the polyculture of seaweeds (Saccharina japonica and Gracilaria lemaneiformis) and abalone (Haliotis discus hannai). To investigate the environmental effects of fish farming on sediments, surface (0–1 cm) sediments were collected from 41 stations grouped into four areas: fish, seaweed

The Hong Kong oyster (Crassostrea hongkongensis) is endemic and one of the most important economic and extensively cultured oyster species along the southern coastal areas of China. However, limited information is available about its genetic variation and genetic structure. In this study, we analyzed genetic variation at twelve microsatellite DNA loci in eleven locations throughout the distribution

RNA/DNA ratio (RDR) has been reported to be a feasible biochemical indicator for measuring the growth of numerous aquatic animal species. Nevertheless, its applicability has not been investigated to date in the pearl oyster Pinctada fucata (Gould, 1850). A laboratory experiment was undertaken to determine the relationship between RDR and growth rate in P. fucata postlarvae. The face-centered central

The practice of adding probiotics in shrimp diet has increased significantly because of its beneficial effects on growth performance and disease resistance. However, little attention has been paid to marine-derived probiotics, which might be more suitable for aquatic animals. In the present study, an 8-week feeding trial was carried out to explore the effects of Shewanella sp. MR-7 in shrimp diet.

An enrichment protocol was used to isolate and characterize tri-nucleotide microsatellite markers in Crassostrea hongkongensis, an economically important and extensively cultured oyster species along the southern coastal areas of China. Thirty tri-nucleotide microsatellite loci were assessed in 32 individuals from a wild population. The number of alleles ranged from 2 to 12, averaging 7.63 alleles

The present study was conducted to evaluate the effects of dietary lipid levels on lipid metabolism, growth performance, antioxidant ability and intestinal morphology of rainbow trout (Oncorhynchus mykiss) under cage culture with flowing water. O. mykiss (average initial body weight 17.64 ± 0.05 g) were fed with six diets (L1, L2, L3, L4, L5 and L6) containing six levels of lipid (10.03 %, 12.97 %

A 60-day study was conducted to evaluate the growth performance, nutritional quality of edible tissues (muscle and hepatopancreas) of red swamp crayfish (Procambarus clarkii) cultivated using commercial diet feeding (diet group) and biofloc technology (biofloc group). The commercial diet used in the biofloc group was only 60 % of that in the diet group. Glucose and wheat bran were used as additional

The present study reports the first incidence of intersex in Cobia, Rachycentron canadum maintained at sea cages in the Gulf of Mannar, India. During the 10 year study period (2010–2019), at total of 815 broodstock (386 wild captured and 429 captive raised) were maintained under captivity in sea cages in the Gulf of Mannar, India for the purpose of seed production. Of which, 318 male and 252 female

The emerging microsporidian parasite Enterocytozoon hepatopenaei (EHP) causes retardation of shrimp growth, leading to significant financial losses in shrimp aquaculture. Therefore, the development of an efficient and sensitive detection method will be conducive to the prevention and control of the shrimp parasite. In this study, we developed and evaluated a rapid real-time recombinase polymerase amplification

This study aimed to analyze the extrusion processing potential of glandless cottonseed meal (GCSM) in extruded shrimp feed. Five different diets with different fish oil concentration and constant GCSM content were extruded. Extrusion temperature, screw speed, and moisture content were varied. Optimal extrudates with GCSM had 50.5 % of crude protein, which is higher than commercial shrimp feed. Mineral

The present study conducted on evaluating the effects of feeding with molasses along with exposure to copper nanoparticles (CuO-NP) on blood, serum and mucus immunity indices of Cyprinus carpio. The results showed that the blood indices, serum and mucus immunity enhanced significantly compare to the control group (P < 0.05). CuO-NP is a compound with sevral negative effects on blood, serum and mucus

Cytochrome P450s (CYPs) are present in almost all organisms and play critical roles in metabolism of endogenous substrates and detoxification of xenobiotic compounds. Oysters are a group of bivalves that are important in marine ecosystem and aquaculture program, adapting to the harsh environment in intertidal zones. The studies on CYPs and their potential involvement in dealing with toxic xenobiotics

Glucose-6-phosphatase (g6p) plays a crucial role in glucose homeostasis by completing the final step of gluconeogenesis in mammals. Multiple g6p homologous genes were identified in fish due to whole genome replication. However, the g6p homologous genes closely related to glucose metabolism have not been systematically screened and the DNA methylation of g6p homologous genes induced by dietary carbohydrates

Nile tilapia (Oreochromis niloticus) is a well-known economic fish species and can grow well under optimum environmental conditions. However, stressful rearing conditions are expected to deteriorate the performance and health condition of fish. Hence, the present study investigated the physiological and ion changes of Nile tilapia under salinity stress. Fish of 200 ± 20 g were divided into three groups

This investigation revealed the first biological bases of embryonic development until the hatching of South Pacific bonito Sarda chiliensis chiliensis eggs. The characteristics and stages of embryonic development were described until the hatching stage. The eggs were obtained by natural spawning from wild-caught broodstocks that were conditioned to captivity for more than 1 year in a 75 m3 marine land-based

This work aims to evaluate the effect of transport stress on the hematological and biochemical responses in males and females of bullfrogs (Lithobates catesbeianus). Frog conditions were evaluated before and at five times after transport (0, 6, 12, 24, and 48 h) for both genders. Glycemia increased in the frogs evaluated at time 0 h and recovered 24 h after transport. Lactate increased in the animals

This research describes for the first time, and necessary for its practical application in aquaculture, the morpho-physiological development, the allometric growth patterns, and behavioral characteristics of the early stages of larval development of the South Pacific bonito (Sarda chiliensis chiliensis). Bouyant eggs, from natural spawning, were collected from the conditioning rearing tank where wild-broodstocks

Nowadays, effective microorganisms (EM) is wildly employed in water improvement and agricultural utilization. Many experiments in aquaculture have proved that EM takes positive effects on fish. Naturally, the present experiment evaluated the influence of supplemental EM in feed on tilapia, which contains the growth, immunity, and appetite regulating of juvenile GIFT tilapia. Three different EM-concentration

Plant associated yeasts promote growth and protect plants from various pathogens in multifaceted mechanisms. However, no report has so far been published on the effect of plant endophytic yeasts on growth and development of fishes. This study aimed to isolate and identify endophytic yeasts from pineapple (Ananas comosus) and evaluate the effects of these yeasts-enriched diets on growth, nutrients of

This study assessed the nutritional potential of supplementing the practical diets for gibel carp (Carassius auratus gibelio var. CAS V) with a blend of fermented plant meal. The fermented plant meal (FPM) comprised mixed fermentation of rapeseed meal (RM), sprayed corn husk (SCH), rice bran (RB), palm meal (PM) and soybean meal (SM) in the proportion of 30:25:25:15:5. Four experimental diets were

Hypoxia-inducible factor-1α (HIF-1α) is a key transcription factor for regulating oxygen-dependent homeostasis. Here, we characterized HIF-1α in Pacific abalone (Haliotis discus hannai), an aquaculture-relevant mollusk species, and its transcriptional regulation patterns in response to oxygen-dependent and oxygen-independent stimuli were investigated. The molecular cloning and sequence analysis revealed

The dissolved oxygen (DO) level is particularly important for marine benthic crustaceans. In Scylla paramamosain, the DO level plays an important role in regulating immune and metabolic responses. The present study assessed whether the effects of hypoxia on the resistance of S. paramamosain to Vibrio alginolyticus are related to the changes in energy metabolism. In hypoxic S. paramamosain, the immune-related

This study aimed to determine the impact of immersing pikeperch (body weight approximately 94 g) in an aqueous solution of sodium chloride on gasometric, hematological, and biochemical indicators. The impact of the sodium chloride concentration (NaCl 10, 15, and 20 g L−1), immersion time (30 or 60 min) and time of blood sampling (0 h – blood drawn immediately following fish immersion; 24 h – blood

High-mobility group box1 (HMGB1) is a non-histone chromosome protein that not only participates in nuclear formation and transcriptional regulation of, but also acts as a pro-inflammatory factor. In this study, a HMGB1 gene (OnHMGB1) was identified and characterized from Nile tilapia (Oreochromis niloticus). The open reading frame of OnHMGB1 sequence was 621 bp, encoding a 207 amino acids peptide.

Non-O1 Vibrio cholerae is a highly virulent pathogen which has seriously threatened the development of Macrobrachium nipponense in various farms. However, little information is available in relation to underlying mechanisms of host-bacteria interaction in M. nipponense. In this study, we performed transcriptome analysis of M. nipponense at 6 and 12 h post-infection (hpi), and differentially expressed

Six iso-nitrogenous and iso-lipidic experimental diets were formulated to evaluate the effects of Saccharomyces cerevisiae extracts, as part of a low fishmeal diet with a complex plant protein source, on the orange-spotted grouper (Epinephelus coioides). In positive control group, fishmeal was the only protein source (FM). The negative control group (Y0) was fed low amounts of fishmeal, and plant protein

A ten-week feeding trial was conducted to investigate the effects of the supplementation of Clostridium autoethanogenum protein (CAP) in practical diets on the growth performance, serum chemical parameters, antioxidant status and histopathological examination of the liver and midgut in juvenile Jian carp. Five isnitrogenous and isenergetic diets were formulated with graded levels of CAP (0, 50, 100

Salinity is an important environmental factor that has a profound impact on the growth, reproduction, survival, and development of mollusk. To investigate the molecular changes in response to acute low-salinity stress, the clams were transferred from 25‰ to 8‰. According to the exposure times of 0, 12, 24, 48, 72, 96, and 120 h, the treatment consisted group T0 (the control), T1, T2, T3, T4, T5, and

Protein is the most expensive nutrient in aquafeed. Cottonseed meal can be used as an important source of plant protein in aquafeed because of its low price and high yield. Concentrated dephenolized cottonseed protein (CDCP) is a promising alternative source of fish meal obtained by processing cottonseed meal to remove gossypol. In this study, an 8-week feeding experiment was conducted to evaluate

This study aimed to evaluate the effects of essential oil (EO) of Lippia sidoides, Ocimum gratissimum, and Zingiber officinale in the diet of tambaqui (Colossoma macropomum) growth, hematological and immunological parameters. The survival rate was also calculated after challenge with Aeromonas hydrophila. Seven diets containing 32 % crude protein were formulated as follows: control (0 g/kg of EO),

Plant proteins are widely used for fish meal replacement in aquafeeds, but anti-nutritional factors in plant protein reduce fish growth performance and impair fish health. The present work aimed to study the effects of improving fish meal replacement percentage with ultra-micro ground mixed plant proteins (uPP) on growth, gut and liver health of common carp. Carps were fed with a practical basal diet

Lumpfish is used to control sea lice in open net-pen farming of Atlantic salmon, but little is known about their nutritional requirements. The aim of this study was to investigate the effects of replacing marine oil (MO) with rapeseed oil (RO), in diets incorporating 50 % plant protein concentrates, on the growth, chemical and fatty acid (FA) composition of juvenile lumpfish. Four extruded diets, nearly

Information on allometry, condition indices (CIs), and biometric traits (BTs) of marine bivalves are necessary for determining the growth pattern and the size at which harvest can be intensified to maximize production in aquaculture. These parameters of marine bivalves are largely regulated by the complex interactive effects of various ecological and physiological factors. Therefore, the present study

The increase in overfishing and pollution in Pátzcuaro Lake, Michoacán, México, has generated a severe problem in the endemic species that live there; especially the Chirostoma estor estor that has been threatened. A computational model has been developed for the evaluation of water quality in freshwater intensive farming tanks to contribute to the conservation efforts of this species. The proposed

Piwi is necessary for germ cell development in a diverse range of organisms and homologues have been commonly used to identify primordial germ cells (PGCs) during embryogenesis. Here, we isolated full-length cDNA of Piwi ortholog in the Pacific oyster Crassostrea gigas and characterized its expression patterns (Cg-Piwi-like) along with analyzing the expression alternation in the gonad of diploids compared

Fungal infections have been reported as one of the main hindrances for successful crustacean seed production. In a shrimp hatchery of Universiti Malaysia Sabah, abnormal sponge development of the ornate spiny lobster Panulirus ornatus was observed and it was suspected as fungal infection due to a change in sponge colour. Fungus was isolated from egg samples of P. ornatus and transferred to Peptone

Neobenedenia girellae is considered an epizootic infection for intensively cultured fish species. Particularly, for greater amberjack (Seriola dumerili) N. girellae causes high mortality rates and supposes a bottleneck during its on-growing period. Thus, the objective of this work was to describe the skin morphological alterations caused by a N. girellae infection on greater amberjack. Greater amberjack

At present, the methods for monitoring the health status of fish mostly utilize blood biochemical indicators. As the blood collection process often causes damage to the fish body (sometimes causing death), the need to develop a noninvasive detection method is urgent. Previous studies have shown that stress indicators can be detected in fish skin mucus or the surrounding water. In this experiment, the

Condensed tannins (CT) have been observed to be beneficial to maintain glucose homeostasis and improve carbohydrate utilization for livestock, whereas little information is available about the effects of CT on glycometabolism of fish. Three diets were formulated to contain approximately 15 % carbohydrate (G1), 39 % carbohydrate (G2), and G2 supplemented with 0.1 % CT. A total of 225 juvenile Lateolabrax

This study analysed the effect of feeding a freshwater fish, barbel (Barbus barbus), with Artemia sp. enriched with polyunsaturated fatty acids (PUFA). Barbel larvae were divided into two groups, which received a daily dose of nauplii at 50 % of the fish body weight. One of the groups received nauplii enriched with PUFAs (group E), whereas the other received nauplii without enrichment and was used

Aquaponics is the integrated production of aquatic organisms and plants. The majority (>50 %) of nutrients sustaining optimal plant growth in aquaponics is derived from waste originating from the feeding of aquatic organisms kept in aquaculture. Currently, aquaponics is more popular as a hobby rather than for commercial-scale production. We used long-term data verification to present a qualitative

Aquaculture effluent is often associated with increased organic carbon, suspended solids, phosphates, nitrogenous species (nitrates, nitrites, and ammonia), chemical oxygen demand and biological oxygen demand. This is regarded as a global threat to aquatic ecosystems due to its influence on surrounding waters as well as groundwater. The threat of aquaculture effluent is not confined to the aquatic

This study investigated the effects of rapeseed meal (RM) as fish meal (FM) alternative on the growth rate, feed utilization, protein metabolism-related parameters, and antioxidant indices of Asian red-tailed catfish (Hemibagrus wyckioides). Five isoproteic and isocaloric diets were formulated with 0%, 11.2 %, 22.4 %, 33.6 %, and 44.8 % RM replacing graded levels of FM, respectively. Each experimental

This study provided a preliminary framework for the effects of Origanum vulgare L. essential oil (EO) on sea bass (Dicentrarchus labrax) health status over a 60-day feeding trial. Fish were fed twice a day until apparent satiety with three different diets: a control diet (CD), and two experimental diets supplemented with 100 (D100) and 200 (D200) ppm of oregano EO. No mortality was observed in each

A 60-day feeding trial was performed to test the nutritional value and utilization possibility of palm kernel meal (PKM) as a feed ingredient for juvenile Labeo rohita. Five iso-nitrogenous diets were prepared with various inclusions of PKM which replaced sunflower oil cake and de-oiled rice bran namely, control (without PKM), T1 (50 g/kg PKM), T2 (100 g/kg PKM), T3 (150 g/kg PKM) and T4 (200 g/kg

An experiment was carried out to evaluate the growth performance and yield of Labeo bata reared in cages at different stocking densities in a floodplain wetland of Assam, India. Fifteen cages (effective water volume 40 m3 each) installed in the wetland were stocked with fingerlings of L. bata (mean length 8.20 ± 0.08 cm; mean weight 4.82 ± 0.13 g) at five different stocking densities, i.e., 25 (D25)

At present, the application of probiotics in aquaculture is gaining wide acceptance for its beneficial effect on fish production. This study focused on evaluating the potency of a commercial multi-strain probiotic on mean growth, feed utilization parameters, yield and survival for profitable cage culture of striped catfish, Pangasianodon hypophthalmus at haor floodplain of Bangladesh. The present study

The optimal mixed feeding schedule for mono-sex male Nile tilapia (Oreochromis niloticus) was investigated in the current study. The fish (4.01 ± 0.02 g initial weight and 5.84 ± 0.04 cm initial length) were fed up to satiation with high protein (HP, 32.80 % protein on dry basis) and low protein (LP, 28.64 % protein on dry basis) diets in different schedules: alternately every other day (1HP/1 L P)

A feeding trial was conducted to investigate the effects of Chinese herbal medicines mixture (CHMM) on growth performance, digestive enzyme activity and serum biochemical parameters of European eels, Anguilla anguilla (initial weight 6.19±0.09 g). Diets with different supplementation of CHMM (0, 2.5, 5, 7.5, 10, 12.5 g/kg) were given to eels for 42 days. Results showed: eels fed with 5–12.5 g/kg CHMM

This study assessed the activity of the main digestive enzymes in juveniles of the painted river prawn Macrobrachium carcinus. Prawns reared in 24 m2 tanks at a density of 10 individuals. m−2 for 45 days were fed a commercial diet with 40 % crude protein. A sample of 21 prawns with 2.92 ± 0.60 g and 27.87 ± 1.50 mm had their midgut glands (MG) dissected. Assays prospecting total proteolytic activity

The study explored the effects of extract of mulberry leaves (EML) on the growth performance and hypoxia-reoxygenation stress as well as biochemical parameters in various organs of crucian carp (Carassius auratus gibelio). The results indicated that dietary EML improved the growth of crucian carp. The appropriate concentration of EML for fish growth was estimated to be 46.93 g kg−1 diet. Moreover,

Recently, the concept of precision nutrition has emerged in aquaculture, and there is increasing discussion on this topic. Some researchers have defined precision nutrition as precision feeding, which means that the nutrient contents of feed should meet the requirements for an animal’s optimal production performance and achieve minimum fodder waste at the same time. In practice, precision nutrition