There is currently a global high market demand for soft-shell crabs, which are priced much higher than crabs in hard-shell conditions. In order to harvest crabs in soft-shell conditions, they are reared and collected as soon as they molt, and limb autotomy is commonly used to hasten the molting process. In this study, we compared full limb autotomy (the removal of all the appendages except for the

This study was to evaluate the effects of substituting fish meal (FM) with soy protein concentrate (SPC) on growth, non-specific immune function, antioxidant capacity and intestinal histology in juvenile golden crucian carp, Cyprinus carpio × Carassius auratus. Six isonitrogenous (40 % crude protein) and isolipidic (8 % crude lipid) diets were formulated with SPC to replace 0 % (FM control; SPC0),

This study investigated the effects of cyclical fasting and refeeding on growth performance, digestibility, muscle composition and mTOR signaling pathway of Penaeus vannamei. Three feeding strategies were designed: feeding everyday of a week (control); fasting one day and refeeding six days of a week (F1), fasting two days and refeeding five days of a week (F2). Shrimp with initial body weight of 6

Anti-nutritional factors can be substantially reduced with processing to increase the utilization of feed ingredients. In this study, hydrothermally processed Jatropha curcas kernel (JCK) (62 min) was subjected to a natural un-inoculated fermentation process for varying durations (7, 14 and 21 days) to improve their nutritional profile and utilization by African catfish Clarias gariepinus (Burchell

Our objective is to study the low metabolic utilization of dietary carbohydrates in fish. The regulation of key enzymes involved in glucose and lipid metabolism in the common carp (Cyprinus carpio L.), known to be relatively tolerant to dietary carbohydrates, were assessed. We performed intraperitoneal (i.p.) administration of insulin and glucagon to common carp and also subjected common carp primary

Magur (Clarias magur) is an Indian catfish species with a good potential for aquaculture. The expansion of magur aquaculture is hindered because of low reproductive and survival rates. Furthermore, males need to be sacrificed to collect milt for artificial fertilization. At present, magur seed production mainly depends on the wild-caught juveniles and to a smaller extent, from broodfish whose genetic

A 4-week feeding trial was conducted to assess the effect of AA on growth performance, hematological parameters, antioxidant and non-specific immune response in starry flounder, Platichthys stellatus (mean weight, 139.47 ± 1.41 g; mean length, 19.87 ± 0.21 cm). AA diets were designed as 0, 50, 100, 200, 400, 600 and 800 mg/kg, supplied as l-ascorbyl-2-monophosphate (AMP). The results of present study

Triploid rainbow trout (Oncorhynchus mykiss) raised increasing concern on the commercial farming due to its prominent advantages, such as improved growth performance and efficiency of feed utilization. It typically shows impaired ovary development and is relatively sterile. Spindlin-1 (Spin-1) plays vital roles in hormone regulation, gametogenesis, and oocyte meiotic resumption in almost all vertebrates

In this study, CPT Іα cDNA of obscure puffer (Takifugu obscurus) was cloned and characterized, and its expression in response to replacement of dietary fish oil with vegetable oil was investigated following a feeding trial. The sequence of obscure puffer CPT Іα cDNA contains a 5’ UTR of 113 bp, a 3’ UTR of 294 bp and an open reading frame of 2316 bp encoding a polypeptide of 771 amino acids with an

Over the past decade, the ivory shells have been successfully exploited as important aquaculture organisms in Southern China. In this research, we measured the annual growth of shell length and body weight of two species including Babylonia areolata and B. lutosa (CBL) of ivory shells. a Chinese populations of B. areolata and B. lutosa, and a Thai population of B. areolata were studied. We used Mallows’

Prothymosin alpha (ProTα) is a special protein without tertiary institutions. In this study, we focus on the stability of ProTα obtained from Chinese human genes which is slightly different from other reported ProTα genes. Quality standard, isoelectric point, mass spectra and bacterial endotoxins were tested to confirm the properties of the protein. We applied ProTα to the aquatic feed of Penaeus vannamei

Litopenaeus vannamei has a salinity tolerance range of 0.5–78 ppt. In China, cultivation of L. vannamei in high salinity waters in the Circum-Bohai Region resulted in low survival rates and yields because high salinity has toxic effects on shrimp. Thus, identifying differential gene expression in different tissues of L. vannamei under high salinity stress would help revealing its salt tolerance mechanisms

This study evaluated the in vitro and in vivo parasiticidal efficacy of the essential oils of Mentha piperita, Lippia alba and Zingiber officinale on Neoechinorhynchus buttnerae (acanthocephalan) and their effect when used as dietary supplementation on hematological parameters of Colossoma macropomum. For the in vitro assay, the parasites were exposed to five different concentrations (360, 540, 720

The present study evaluated the effects of dietary yeast supplementation on the growth and blood parameters of Arapaima gigas. Fish were fed with commercial feed supplemented with NuPro® at 0, 10, 40 and 80 g / kg for 30 days and, did not exhibit any difference in growth. Blood was analyzed at the end of the feeding trial and after handling stress. A significant reduction in albumin and triglycerides

Hybrid Malaysian mahseer is a crossbreed between the male Malaysian mahseer, Tor tambroides and female silver barb, Barbonymus gonionotus through induced breeding. The study was carried out to observe the morpho-histological development of the F1 hybrid Malaysian mahseer larvae for the entire duration of its larval stage. Newly hatched larvae were reared in three 120 L aquaria at a stocking density

The nutritional study was performed for 90 days to assess the potential protective effect of three concentrations (0.5, 1, and 3 mL/kg) of d-limonene derived from orange peel essential oil against Yersinia ruckeri in rainbow trout. Mean corpuscular volume, mean corpuscular hemoglobin, serum glucose, alanine aminotransferase, total protein, and globulin were unaffected by any d-limonene treatment group

Fishmeal is an important source of protein in the fish diet, but its supply is unstable due to environmental pollution and overfishing of anchovy and herring. In addition, the disease outbreak of fish has been increased, and the abuse of antibiotics and vaccines has been becoming a problem for residual substances. Therefore, studies that address diets with reduced fishmeal content and additives that

Due to increasing demand of ingredients of animal origin, studies on the availability of nutrients in ingredients of plant origin are worthy in carnivorous species including bullfrog. This study aimed to determine apparent digestibility coefficients (ADCs) of amino acids (AAs) in three ingredients as plant protein sources (soybean protein concentrate (SPC), corn gluten meal 60 % (CGM), soybean meal

The natural production of the native flat oyster Ostrea edulis and the production of seed in hatcheries are insufficient to meet demand. Several projects have recently been developed to optimise flat oyster production and to establish a reliable protocol for broodstock conditioning in hatcheries. In this sense, the positive effect of a gradient of temperature and a gradient of daylight on the gonadal

Growth hormone (GH) participates in many physiological processes through the activation of its membrane-anchored receptor (GHR) in vertebrates. Because of its pivotal role in regulating growth and development, a large number of studies examining GH-GHR have been conducted in mammals, birds and fish. However, whether a functional GH-GHR pair exists in the Chinese soft-shelled turtle (st-) has not been

For several years Tunisia has opted to breed freshwater fish in reservoirs and artificial lakes, created for irrigation, as a strategy of providing high quality aquatic protein to the interior regions and providing work opportunities for local communities. The aim of this study was to summarize the main results accumulated by the last 20 year efforts made by the members of the National Institute for

Classical cadherin gene family relies on calcium ions in playing an important role in adhesion between cells. However, comprehensive studies to identify classical cadherin gene families in Nile tilapia (Oreochromis niloticus) have not been conducted. In this study, 18 classical gene family members were screened from Nile tilapia using various bioinformatics methods. These screened cadherins were randomly

Lead, an environmental toxicant in our daily life, has been reported to have adverse effectson human and animals. Previous studies showed lead could accumulate in kidney tissues of carp and cause injury to the kidney tissues. In this study, we aimed to investigate the protective effects of andrographolide on lead-induced kidney injury in common carp and to clarify the possible mechanism. The results

In this study, we examined DNA sequences of bacteria isolated from goldfish intestine and identified the species in collaboration with the Food Industry Research & Development Institute of Taiwan. One bacterial species, Enterococcus avium, was selected for evaluation of its antimicrobial effects and probiotic function. The antimicrobial effects of feed containing E. avium were investigated in tilapia

To investigate the effect of dietary folic acid (FA) on growth performance, proximate composition, blood parameters, antioxidant status, and immune response of Siberian sturgeon, Acipenser baerii, six isonitrogenous and isolipidic experimental diets were formulated to contain graded levels of FA (0.60, 1.63, 2.71, 3.59, 4.82, and 5.68 mg kg−1). Each diet was fed to triplicate groups of fish (4.36 ± 0

Estradiol is a steroid hormone that regulates multiple physiological processes in teleosts. The present study aimed to investigate the role of 17β-estradiol (E2) in lipid metabolism in the liver of Nile tilapia. Waterborne exposures were performed using different concentrations of E2 (0 ng/L, 10 ng/L, 25 ng/L and 50 ng/L) and its receptor inhibitor ICI 182,780 (ICI) (1 μM/L). Subsequently, the survival

The cost of fish feed is a major constraint to fish farming in Sub-Sahara Africa. In the aquaculture value chain, feed is a determining factor and accounts for 60-75% of the total cost of fish production in many African countries. Therefore, 284 actors from all eight agro-ecological areas of Benin were interviewed and 28 local feed ingredients were collected as alternative ingredients for new fish

This study was aimed to evaluate the effects of dietary supplementation of probiotics Bacillus cereus (BC), B. subtilis (BS), Paracoccus marcusii (PM), and Lactobacillus plantarum (LP) on growth, immune response, antioxidant property, and intestine health of juvenile grass carp. Our results showed that dietary probiotics could promote the growth performance, which was significantly increased in BS

Mid1ip1 plays important roles in lipogenesis and microtubule stabilization, but little relevant information has been reported in fish. In the present study, mid1ip1 cDNA of tiger puffer was cloned and characterized, and its expression in response to dietary bile acid and lipid levels was investigated following a feeding trial. Experimental diets with suitable (8.5 %) or high lipid level (12.5 %), with

Rapana venosa is an economically important aquaculture species in China. Carboxypeptidases (CPs) A and B (CPA and CPB, respectively) hydrolyze peptides and proteins and play critical roles in the development of the digestive system in animals. In this study, a full-length CPA-like cDNA sequence from R. venosa (RvCPA) was isolated using RACE, and RvCPA mRNA expression levels were analyzed in different

A three-month feeding trial was conducted to investigate the effects of faba bean (Vicia faba L.) on growth performance, intramuscular collagen content, muscle cellularity and fillet texture of Yellow River carp, Cyprinus carpio haematopterus. Carps with an initial weight 500 ± 20 g were randomly divided into two treatments: commercial feed group (CFG) and faba bean group (FBG). Compared to CFG, the

Fish oil can not only provide energy for the growth and development of fish, but also constitute a variety of tissue and cellular structural components of fish. However, fish oil is easily oxidized in the process of feed processing, treatment and storage, which reduces the feed quality and threatens the health of fish. Therefore, the purpose of this study was to investigate the effects of different

Marennine is the blue-green pigment produced by the temperate ubiquiste marine diatom Haslea ostrearia. This pigment is water-soluble and excreted in seawater during H. ostrearia growth and ageing. Previous studies demonstrated the adverse effects of H. ostrearia culture supernatant that contains marennine (Blue Water, BW) against marine organisms. In tropical waters in the Southern hemisphere, a new

Probiotics and herbals are highly recommended in aquaculture; however, little is known about their combined effects. Therefore, the present study evaluated the possible synergistic impacts of Spirulina platensis (SP) and Bacillus amyloliquefaciens (BA) on the growth performance, histomorphology, and immune response of Nile tilapia (Oreochromis niloticus). A control and three test diets were enriched

This study was conducted to determine the minimum number of spermatozoa and optimal volume of activation water for effective fertilization of sterlet Acipenser ruthenus eggs. It was also to determine the minimum concentration of spermatozoa per ml of activation water during artificial propagation, when sperm with good motility and velocity (95 % motility and 150 μm s−1 curvilinear velocity) were used

As one of the key enzymes that maintain the dynamic balance of metal content in animals and take part in the process of heavy metals detoxification, metallothionein (MT) is also associated with the process of immune response. In this study, the full-length metallothionein cDNA sequence of 324 bp from Mytilus coruscus was obtained (named as McMT) with a 222bp ORF encoding 73 amino acid residues in length

Frass, a by-product of the larval meal industry, is heterogeneous and includes larval excrement, exoskeleton sheds and residual feed ingredients along with abundant nutrients, chitin and beneficial microbes. The present study was performed to evaluate the changes in growth, feed utilization, body composition, hematology, serum chemistry, immune responses and disease resistance of hybrid tilapia, Nile

The use of non-plant and plant-derived anesthetics in aquaculture activities is a frequent and necessary practice to maintain fish welfare. Currently anesthetics authorized for use in aquaculture activities are limited, and the use of unauthorized products is common. However, to obtain authorization for use in aquaculture and ensure their safety, anesthetics should be evaluated for their genotoxic

Pathogenesis and immune gene expression of the iridescent shark catfish Pangasianodon hypophthalmus to experimental Edwardsiella tarda infection was studied. Healthy Pangasianodon were inoculated intramuscularly with a sub-lethal dose of 1.77 × 107 cells/fish of E. tarda to induce edwardsiellosis. Petechial haemorrhages, inflammation at the site of injection, vertical hanging from the water surface

During the evolution of aquatic animals, co-evolution between host and microbes has been overlooked. Previous studies have shown that microbes were present in the fertilized egg of fish, but compared with fish, little information is available for crustaceans. High-throughput Illumina Miseq DNA sequencing of the V3-V4 domain of the 16S rRNA gene was used to assess the microbial community composition

This study investigated the influence of probiotic Pediococcus acidilactici MA 18/5 M on the metabolic enzyme activities and mRNA levels of growth, immunity, and antioxidative-related genes in zebrafish. Zebrafish fed with diets containing P. acidilactici MA 18/5 M at 0, 107, 108, and 109 CFU/g levels for eight weeks. The obtained results revealed that the creatine phosphokinase, lactate dehydrogenase

The winged pearl oyster (Pteria penguin) is one of the most potential species used to produce large sized pearls. Waterless transport and nuclei implant process are two common handling practices for pearl oyster in aquaculture. These processes can cause air exposure stress. In order to investigate the physiological energetics and oxidative stress of pearl oysters exposed to air, live P. penguin were

Six iso-nitrogenous and iso-lipid experimental diets were formulated to evaluate growth performance, intestinal digestive enzyme activity and the transcript expression of nutrition utilization related genes of the pacific white prawn, Litopenaeus vannamei (initial average body weight: 0.45 ± 0.01 g). The positive control group (LP) contains the 250.0 g/kg fish meal (FM) and 0 g/kg yeast hydrolysate

Members of lactic acid bacteria (LAB) have been well-known for probiotic agents owing to their ability to produce diverse beneficial compounds for cultured species, as well as for having a status of generally recognized as safe microorganisms. Considering these facts, the current study investigated the presence of cultivable LAB associated with the gastrointestinal tracts (GIT) of hybrid abalone (Haliotis

This study evaluated the sedimentation rates of nutrients and particulate matter in Nile tilapia (Oreochromis niloticus) net cage farming in the Umari, Brazilian semi-arid reservoir. Sedimentation chambers were installed under net cages which had stocking of 125 and 100 fish m−³ (experimental sites). The natural sedimentation rate of the reservoir (control site) was calculated using a sedimentation

The effects of starvation and re-feeding on Nile tilapia (Oreochromis niloticus) remain incompletely understood so far. In this study, the changes of intestinal microbiota, the metabolic and immune (antioxidant) responses of Nile tilapia (Oreochromis niloticus) during starvation and re-feeding stages were investigated. The metabolic responses (cortisol, ALT, AST, and leptin) and immune responses (hsp70

In the present study, the effects of β-glucan administration in water on growth performance and blood status (hematological indexes, immunological and biochemical parameters) were evaluated in Nile tilapia fingerlings (Oreochromis niloticus) under hypoxia-induced stress. In parallel, effect of this prebiotic (beta-glucan) was evaluated on gut microbiota diversity (16S rRNA gene) under normal oxygenation

The present study was conducted to replace fish meal with alternative plant protein sources in aquafeeds. Therefore, soybean meal (SBM), corn gluten meal (CGM), and mix of SBM + CGM were used as alternatives to fish meal (FM) with or without betaine (B) in Nile tilapia diets. Positive control diet using FM and six other diets were formulated to contain SBM, SBM + B, CGM, CGM + B, SBM + CGM, or SBM + CGM + B

This work aimed to determine fatty acid dynamics and lipid composition of female muscle, oocytes, newly hatched larvae (NHL) and first feeding larvae (FFL) of Prochilodus argenteus. Oocytes and NHL had the highest percentages of total lipids. Neutral lipids (NL) and polar lipids (PL) remained constant from oocytes through the larval stages (P < 0.05). Among NL, C18:2n 6 had its highest percentage in

Acute cold snaps induce a sudden reduction in water temperature and lead to high mortality of cultured Pacific white shrimp (Litopenaeus vannamei); however, little is known about the regulatory mechanisms in this shrimp species when adapting to acute cold stress. Mitogen-activated protein kinase (MAPK) signaling pathways are highly conserved and well known for their important roles in transducing environmental

We evaluated the growth and body traits of pacu and tambacu hybrid reared in cages and fed through different feeding programmes. Six treatments in a factorial scheme, with two genetic groups (pacu and tambacu) and three feeding programmes, were tested in 12 cages. In each cage, 50 juveniles (pacu, 120 ± 31 g; tambacu, 119 ± 28 g) were stocked and fed according to three feeding programmes using three

Peroxygen-based chemotherapeutants are commonly used in treating ectoparasitic infections in fish. A majority of relevant studies address the effects of these substances on the targeted causative agents, but little is known about their physiological impacts on the host organism. This study documented the changes in the skin of Atlantic salmon (Salmo salar) exposed to peracetic acid (PAA), a peroxygen

Bacillus spp. as probiotics have been widely used in the aquaculture industry. This study was designed to investigate the dietary supplementation of Bacillus cereus QSI-1 with quorum quenching activity, on intestinal barrier and expression levels of innate immune genes. After 14 d of feeding, fish fed with Bacillus cereus QSI-1 exhibited a significant down-regulated gene expression of TNF, IL-8, and

The growth performance and muscle quality of tilapia (Oreochromis niloticus) were compared between a fish-rice co-culture system (SD3) and a monoculture system (CG3). Tilapia with initial mean weight of 10 g and mean length of 5 cm raised in improved water quality produced by the co-culture system (SD3) showed better growth parameters, as well as better proximate composition with higher protein and

Probiotics can be used to reduce disease outbreaks in aquaculture. Some of them are characterised by their antagonistic activity against pathogens or the stimulation of the fish immune response, including the production of specific antibodies. If a probiotic has common antigens with a determined pathogen, it could produce antibodies with a cross-reaction to that pathogen. Thus, a probiotic with these

Mabé pearl culture is an increasingly important rural livelihood in south Pacific countries as it offers a low-cost, low-tech alternative to round pearl culture. Mabé pearl production can be achieved by local people with appropriate training, and the products offer further livelihood opportunities through value-adding and local production of jewellery and handicraft items. The Kingdom of Tonga is unique

The effects of Azomite, a natural mineral of volcanic ash, on the sex steroid hormone (SSH) levels and milt quality indices (MQI); namely, the milt volume, motility of spermatozoa, its duration and sperm count, were evaluated in tilapia (Oreochromis mossambicus). Several reports have documented the positive effect of the Azomite supplementation on growth, survival, and immune response of aquatic organisms

Crumbled feed (CF) is the most commonly used microdiet in the weaning phrase of large yellow croaker (Pseudosciaena crocea) in China. However, CF can be inefficient for local hatcheries due to its low water stability. In this study, we evaluated a more water-stable microdiet (micro-extrusion marumerization diet or MM) in comparison to the CF, and ascertained their effects on the growth of larval croaker