Understanding factors affecting the reproductive performance of mud crab (Scylla paramamosain) is important for the optimization of the culture conditions. There are some indications that complete darkness could enhance and accelerate the spawning in female decapods, but sometimes it may lead to low larval quality. Therefore, the present study was carried out to clarify the effect of different light

Temperature and light intensity are two closely related and inseparable key environmental factors in natural cultivation of Chaetoceros calcitrans. Herein, two temperatures (20 and 30 °C) coordinated with three light intensities (40, 120 and 200 μmol m−2 s−1) were employed to C. calcitrans cultivation. During which, the growth rate, photochemical efficiency, antioxidant ability and photosynthetic pigment

Woody forage as potential aquatic feed ingredient has a positive effect on tilapia survival and feed utilization. To study the effect of woody forages on the growth, feed utilization, apparent digestibility, intestinal morphology and microbiota composition of Nile tilapia (Oreochromis niloticus), the diets of Moringa oleifera Lam (MOL), Folium mori (FM), Broussonetia papyrifera (BP) and Neolamarckia

Snubnose pompano (Trachinotus blochii) are widely cultured in the world. Light colours are among factors affecting fish performance in captivity. Snubnose pompano larvae often reared under white light. However, no study supports its choice or informs the extent of its effect or its constituent colour. The study investigated the influence of green, blue, yellow, purple, and white lights on growth, survival

Ribosome, as basic components of the protein synthesis machinery, plays an important role in normal cellular physiology, cellular responses and pathogenesis. Ribosome consists of a large subunit (60S) and a small subunit (40S). In Litopenaeus vannamei (L. vannamei), the function of the 60S ribosome protein L4A-Like (LvRPL4A-L) and the 40S ribosome protein S16 (LvRPS16) in white spot syndrome virus

Dmrt gene family plays a very critical role in sex determination and differentiation in animals. In this study, dmrt1, dmrt2 and dmrt3 were first cloned from the gonads of mandarin fish (Siniperca chuatsi). The complete open reading frame sequences of dmrt1, dmrt2 and dmrt3 were 891, 1581 and 1419 bp, encoding 296, 526 and 473 amino acids. Multiple sequence alignments revealed that dmrt proteins were

Probiotics, an eco-friendly alternative to antibiotics application appears to have strong potential for improving the sustainability of aquaculture. The aim of the study was to evaluate the effect of probiotic brewer’s yeast, Saccharomyces cerevisiae supplementation on growth performance, feed utilization and intestinal morphometry of Nile tilapia, Oreochromis niloticus. A feeding trial of 60 days

Seabass (Lates calcarifer) are high value fish occupying a market niche, and is susceptible to infection by pathogenic bacteria such as Vibriosis. In this study the effect of intraperitoneal administration of recombinant outer membrane protein (OMP) of inactivated cells vaccine; r-OmpK on hematological parameters for monitoring fish health and histopathological examination of seabass were investigated

The main purpose of the agriculture circular economy is waste recycling with the intended goal of economic efficiency and environmental protection. In this study, the potential use of lemon peel, an agricultural waste, as a functional feed additive in aquafeed was evaluated by estimating the effects of fermented lemon peel (FLP) supplementation in diet on the growth, immune responses, and intestinal

This study focused on the effect of Noni, Morinda citrifolia fruit extract supplementation on the growth and the hepatopancreas function of Whiteleg shrimp, Penaeus vannamei Post Larvae (PL). The crude extract of M. citrifolia fruit was obtained through drying and methanol extraction followed by concentration through rotary evaporation. The extracts were incorporated into commercial shrimp pellet at

An 8-week feeding trial was carried out with various lipid level (2.30 %, 6.31 %, 8.98 %, 12.93 %, 16.08 %, and 18.99 %) diets to investigate the effects on growth performance, body composition, and feed utilization of juvenile spotted knifejaw Oplegnathus punctatus. After the feeding experiment, the weight gain (WG) and specific growth rate (SGR) significantly elevated with raising the dietary lipid

This study aims to investigate the effect of zinc on the sex ratio and intersexuality of Tegillarca granosa, a sequential hermaphroditic bivalve that undergoes sex change during the gonadal inactive stage. Groups of T. granosa in the gonadal inactive stage were first exposed to zinc under indoor aquarium conditions for 5 weeks, and then under wild conditions for 3 months. The proportion of males and

The present study was conducted to evaluate the efficacy of the dietary inclusion of commercial hydrolysable tannins (HT) on the growth performance and haemato-immunological parameters of Pacific white shrimp (Litopenaeus vannamei). Four isonitrogenous and isolipidic practical diets were formulated to contain 0; 0,1; 0,2 and 0,3% of HT obtained from the sweet chestnut (Castanea sativa). The 0,3% HT

Herbal extracts have been proven to improve immunity, growth rate and appetite, as well as being antihepatotoxic they are important alternatives to synthetic antibiotics and chemotherapeutics. In this study, Papaveraceae extracts (PE), as alkaloids (quaternary benzophenanthridine and protopine) source were incorporated into the diet of striped catfish, Pangasianodon hypophthalmus, to assess their effects

The aim of this study was to investigate the effect of starch content and pellet physical quality on the biological response in Atlantic salmon. Salmon feeds with a large variation in physical quality were produced by the adjustment of dietary starch inclusion (92 g kg−1 or 64 g kg−1), preconditioner oil (0.0 or 11.0 kg h−1), extruder moisture addition (13.5 or 27 kg h−1) and drying temperature (38

The present study aimed to compare the nutritional effects of a mixture of phytoplankton and zooplankton and a formulated pelleted feed on the growth, survival, moulting, immune and organosomatic indices of juvenile marron (Cherax cainii Austin, 2002) cultured for 90 days, under controlled laboratory conditions. The experiment was conducted with two quadruplicated dietary treatments, a pre-selected

The recent increase in aquaculture production has been possible because of production intensification, development of formulated feeds and feed management. However, increased intensification can cause oxidative stress and immune breakdown in shrimp, resulting in poor performance. The problems associated with increased aquacultural intensification can be managed with proper nutrition. Therefore, the

Disease is a significant constraint faced by aquaculture, and its prevention and control bring together a hub of recent research. Several resources and resorts have been applied to prevent diseases in aquaculture. Probiotics are known to be beneficial natural derivatives that have several benefits in aquaculture. Currently, several commercial probiotics are used in the aquaculture industry that contains

The study was conducted to investigate the effects of DL-Methionyl-DL-Methionine (Met-Met) (a dipeptide of two DL-methionine molecules) on growth performance, immune and antioxidative responses for Litopenaeus vannamei fed low fishmeal diet. Tested treatments consisted of positive control diet (PC) with 20 % fishmeal or 7 % fishmeal (basal diet) diets supplemented with different levels of Met-Met (0

During the early development of the Pacific cod, high mortality due to viral infection is observed. To demonstrate the immune responses activated in Pacific cod during this critical period, two cytokines, the proinflammatory cytokine interleukin-8 (IL-8) and the anti-inflammatory cytokine interleukin-10 (IL-10), were identified and characterized in this study. The cDNA of IL-8 was 1047 bp in length

Red sea bream (Pagrus major) is described as one of the marine fishes with high economic value, which is widely cultivated in East Asia. With large-scale and intensive aquaculture, bacterial diseases outbreak more frequently than before. In order to effectively prevent and control Vibrio anguillarum disease in red sea bream, we systematically analyzed the immune response mechanism of the head kidney

Our aim was to assess the blood physiology and antioxidant and immune responses of the olive flounder (Paralichthys olivaceus) following pH changes in the water. P. olivaceus (mean weight 95.7 ± 6.5 g and mean length 21.4 ± 1.3 cm) were exposed to the different pH levels (pH 5, 6, 7, 8, 9, and 10) for 2 weeks. Blood physiology parameters, such as the levels of hematocrit, magnesium, glucose, glutamic

Hydroxyproline (L-Hyp) is amply present in fishmeal but limited in plant-protein sources. This study was conducted to evaluate the effects of supplementation with dietary L-Hyp on the distribution of collagen types in the muscle, and on the texture, survival rate, growth rate, feed utilization, body composition as well as the expression of the gene that encodes the pro-alpha2 chains of type I collagen

Chemokines are a superfamily of chemotactic cytokines that regulate cell activation, inflammation, and immune defense. In this study, chemokine CXCL10 from Nile tilapia (Oreochromis niloticus) was identified and characterized. The open reading frame of OnCXCL10 is 408 bp encoding polypeptides of 135 amino acids. The amino acid sequence analysis of CXCL10 confirms homology with other species and contains

The class B scavenger receptors (scarb) are members of important pattern recognition receptors which play key roles for host immune response to pathogen infection. In the present study, two scarb member 2 paralogous (Onscarb2a and Onscarb2c) were identified and characterized in Nile tilapia. Onscarb2a was identified with an open reading frame (ORF) of 1593 bp which encoded a polypeptide of 530 amino

Acipenser dabryanus is an endemic species restricted to the upper reaches of the Yangtze River and is listed as a national first-class protected animal in China. In the present study, two lipid metabolism-associated genes, namely, lipoprotein lipase (LPL) and apolipoprotein E (apoE), were identified from the full-length transcriptome analysis of A. dabryanus. The full-length genes encoded polypeptides

Galectin is a kind of lectin that can promote the cell adhesion, proliferation, and phagocytosis and participates in immune regulation and destruction of microbes. In this study, a galectin-related protein (On-GRP) homolog was identified from Nile tilapia, and its expression level and antibacterial ability were studied. The Open Reading Frame, which encoded a 179 amino acid protein (GenBank accession

This study was conducted to investigate the effects of Spirulina and ferrous fumarate supplementations into diets on growth parameters, pigmentation, digestive enzyme activity, antioxidant enzyme activity and fatty acids composition of Yellow River carp. Three experimental groups included control, Spirulina (diet 1), mixture of Spirulina and ferrous fumarate treatments (diet 2) in 3 replications in

Overreliance on fishmeal (FM) as an aquafeed ingredient has become economically and ecologically unsustainable because wild stocks of forage fish are declining causing disruptions in aquatic food webs. On the other hand, the crocodile skin business generates substantial quantities of crocodile meat whose demand for human consumption is extremely low. The potential value of crocodile meat meal (CMM)

This study was conducted to determine the optimal level of defatted black soldier fly pre-pupae (BSFP), Hermetia illucens as a fishmeal replacement in lemon fin barb hybrid diet. Triplicate groups of fish (1.52 ± 0.01 g) were fed twice a day until apparent satiation with five iso-nitrogenous (30 %) and isocaloric (17 kJ g−1) diets containing varying BSFP levels ranging from 0 to 100 % for 8 weeks.

This study demonstrates a robust, flexible interpenetrated composite based on 3D spined fabrics as core material and polydimethylsiloxane (PDMS) as shell material. The penetration of the shell component into the core material enables the mechanical interlocking at the micro and macro scale, providing mechanical stability and at the same time, introducing hydrophobic surface properties. Pure PDMS is

Increasing parentage contribution in aquaculture broodstocks is important, in order to take full advantage of the available genetic makeup of the chosen fish, and to avoid inbreeding and loss of allele diversity over subsequent production generations. European sea bass (Dicentrarchus labrax) broodstocks were evaluated over two reproductive seasons to examine spawning kinetics, egg production, and parentage

This study aimed to evaluate the synergistic effects of Quillaja saponaria (QS), and Vitamin E (VE) on water quality, fish performance, and health of Oreochromis niloticus fingerlings. Fish (N = 120, 12.75 ± 0.289 g) were randomly distributed in triplicates into four experimental groups. (1) the control group (CG), fish received a basal diet; (2) the Quillaja saponaria group (QS), fish received a basal

A highly lethal freshwater crayfish Cambarus clarkii whispovirus (WSSV-Cc) was isolated from a naturally infected host. A large number of viral particles with typical nimavirus characters were observed in host cells. Genomic architecture and genetic evolution of this extremely virulent pathogen were uncovered. WSSV-Cc has a double-stranded DNA genome containing 287,179 bp, encoding 180 putative ORFs

The HIF-1 alpha subunit (HIF-1α) is a master regulator of oxygen sensitivity. This study investigated the role of HIF-1α in the energy metabolism and immune responses of hypoxic Scylla paramamosain. After hypoxia treatment for 12 h, the expression levels of HIF-1α in the hemocytes, gills and hepatopancreas were significantly upregulated. After hypoxia and dsHIF-1α treatment for 12 h, the expression

The spotted sea bass, Lateolabrax maculatus, is a commercially important fish species in China. Microbial communities have important effects on water environment stability and animal health. Therefore, in this study, we comparatively investigated the microbiota characteristics in the water, sediment and gastrointestinal tract of L. maculatus at two growth stages. 16S rDNA high-throughput sequencing

The performance of a commercial starter culture was investigated in a new marine post-smolt RAS, analyzing the microbial communities of 100 samples collected monthly over a year from biofilter biofilm carriers, tank wall biofilm, production water and fish skin. Totally 139 operational taxonomic units (OTUs) were defined in the starter culture, of which the classified members of Rhodobacterales, Bacteroidetes

The aim of this study was to determine the effects of heat-killed Lactobacillus plantarum L-137 (HK L-137) on the growth performances and immune response of juvenile bighead catfish (Clarias macrocephalus). The experiment was conducted with four iso-nitrogenous (44.8 % crude protein) and iso-caloric (20.17 KJ g−1) diets supplemented with different levels (0, 10, 20, and 50 mg kg−1) of HK L-137. The

Nucleotides (NT), as a modern feed additive, are effective in many vital functions of cultured aquatic organisms. An experiment was performed to evaluate the effects of dietary nucleotides on the growth performance, carcass composition, liver enzymatic profile, liver, intestine, and spleen histology in European sea bass, Dicentrarchus labrax (initial weight 29.56 ± 0.083 g/fish). Four isonitrogenous

A 62-day feeding trial was conducted to assess the effects of dietary black soldier fly pulp (BSFP) on growth performance, liver and intestine histomorphology, and liver lipid metabolism gene expression of Micropterus salmoides. Four isoproteic and isolipidic diets were formulated by adding BSFP (original substance) to the basal diet at the rate of 0% (BSFP0), 1% (BSFP1), 2% (BSFP2) and 4% (BSFP4)

Soybean meal (SBM) is a high-quality protein source and widely used in aquafeeds. However, high percentage of dietary SBM would make negative influences on fish. Here, we evaluated the effects of dietary high level of SBM on growth, intestine healthy and intestinal inflammation in large yellow croaker. Two isonitrogenous (450 g kg−1) and isolipidic (100 g kg−1) diets were prepared, in which SBM was

Histidine-containing dipeptides (HCDs) have been demonstrated with important physiological functions in vertebrates. This study was executed to investigate the contents and metabolic profiles of HCDs in an invertebrate, the mussel Mytilus coruscus was selected. The carnosine/anserine analysis indicated that M. coruscus contain anserine with a higher concentration than that of carnosine, and the increases

Micropterus salmoides is one of the most economically valuable fish species in China. Currently, information in the effect of probiotics-supplemented feed on M. salmoides is rare. Present study investigated the impact of supplementary Lactobacillus casei K17 with six treatments on growth performance, gut digestion, absorption and non-specific immune, and gut bacterial biota and metabolomics of M. salmoides

Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) mediating the recognition of peptidoglycan. In the present study, a short-type PGRP gene named Eco-PGRP-S was identified in the orange-spotted grouper (Epinephelus coioides), a major commercial marine fish cultured in Asia. Eco-PGRP-S gene was constitutively expressed in all tissues examined, with the highest

In mammals, the transcription factors paired box-5 (Pax-5) and B-lymphocyte-induced maturation protein-1 (Blimp-1) maintain strict command of B-cell differentiation from hematopoietic stem cells to plasma cells. Here, we identified the Pax-5 and Blimp-1 genes and their predicted proteins in the orange-spotted grouper (Epinephelus coioides). Grouper Pax-5 was predicted to contain a paired box domain

The present study evaluated the enhancement of the growth rate, intestinal health, immune-related genes, and resistance against suboptimal water temperature in common carp (2.01 ± 0.02 g) by dietary paraprobiotics. Fish were fed dietary heat killed Lactobacillus plantarum (LP20) at 0 (control), 50, and 100 mg/kg for 2 months. After the feeding trial fish were exposed to suboptimal water temperature

Shrimp culture economics has a significant influence on the socio-economic status of coastal communities in Bangladesh. Here, we investigated the shrimp farmers' financial and perceptual reactions to analyze shrimp farming's direct impact on their socio-economic status, sustainability, resilience, and the cultural environment. Our outcomes alluded that the farming community's satisfaction level was

Pacific abalone, Haliotis discus hannai (mean length 2.92 ± 0.5 cm, and mean weight 2.63 ± 0.4 g) were exposed to different concentrations of waterborne nickel (0, 100, 200, and 400 μg/L) at 18, 22, and 26 °C for 2 weeks. The exposure concentration of Ni induced a significant Ni accumulation in specific tissues of H. discus hannai depending on water temperature. The order of nickel accumulation in

A 40 day experiment was conducted to investigate the effects of different salinities (0, 5 and 10 ppt) on the growth, osmoregulatory and hypothalamic–pituitary–somatotropic (HPS) axis response of juvenile largemouth bass (Micropterus salmoides). At the same time, two extra groups salinities (8 and 9 ppt) were used to explore the threshold of osmoregulation in M. salmoides to salinity stress. The survival

The swimming crab Portunus trituberculatus is an economically vital aquaculture species in China and South East Asia. Monosex female culture has been identified as a key priority due to their increased market value, consumer preference and enhanced growth performance. However, the lack of a rapid and reliable sex-linked marker panel has hampered the application of sex control and understanding of the

The immune response after transplantation during pearl production is a major factor that cause nucleus rejection and death of the host pearl oysters. To find out the crucial metabolites involved in the immune response after transplantation, we performed metabolomic analysis of the serum before and after transplantation from pearl oyster Pinctada fucata martensii. The analysis detected a total of 201

In 2016, famers reported mass mortality events in hybrid grouper within 2–4 weeks following transfer to sea cages on the northern coast of Bali. The objective was to obtain an aetiological diagnosis using a broad range of traditional and emerging diagnostic approaches. A group of 24 and 12 fish with and without clinical signs were sampled from 10 affected populations at 9 farms. Samples for histopathology

Cathepsin L is an important member of the cysteine protease family and has a central role in the development of ovaries in insects and fish. In this study, we analyzed the characteristics of the cathepsin L1 gene in the female oriental river prawn, Macrobrachium nipponense. The cathepsin L1 cDNA was 1173 bp in length, including a 960 bp open reading frame that encoded 319 amino acids. The cathepsin

This study aimed to evaluate effects of fish protein hydrolysate (FPH) supplementation in plant protein based diets for tiger puffer (Takifugu rubripes) on growth performance, intestinal peptide and amino acid transporters, and the expression of protein synthesis-related genes and texture parameters in muscle. Tiger puffer were fed negative and positive control diets with 280 and 400 g/kg fish meal

A 56-day fish feeding trial was conducted to investigate the effects of dietary Arthrospira platensis on the growth performance, body chemical composition, serum biochemical parameters and antioxidant capacity in hybrid yellow catfish (Pelteobagrus fulvidraco♀ ×Pelteobagrus vachelli♂). Three experimental feeds were prepared. The protein concentrations of these three experimental diets were similar;

This study was conducted to evaluate the effect of acute ammonia stress on intestinal oxidative stress levels, histology, and activity of digestive enzymes and oligopeptide transporter 1 (PepT1) in grass carp. Three concentrations of ammonia nitrogen: 0 mg/L total ammonia nitrogen (TAN) (the control group), 1.7 mg/L TAN were used in the present study. Fish were sampled at 0 h, 12 h, 24 h, and 48 h

To evaluate the effect of stocking density on pre-fattening growth and survival, Manila clam seeds from three different batches (B1, B2, and B3) were farmed in plastic net lanterns at two sites (western and northern) in Sacca degli Scardovari (Italy). Different stocking densities were compared, i.e. EXTRA (50,000 clams m−2), HIGH (30,000), and MEDIUM (20,000) for B1 and B2 clams; HIGH, MEDIUM, and

Ammonia is the mainly environmental pollutant in the field of aquaculture in association with negative impact on the healthy cultivation of common carp. Gene expression profiles in the hepatopancreas of common carp treated with ammonia stress using an RNA-seq technique, and 22,431,825 and 23,592,342 clean reads from the control and treatment groups by a 96-h ammonia challenge (0.85 mg/L unionized ammonia

An 8-week growth trial was conducted to investigate the influences of different dietary carbohydrate sources on growth performance, feed utilization, plasma biochemical parameters and expression of glucose metabolism-related genes in juvenile Chinese soft-shelled turtle, Pelodiscus sinensis. Five isonitrogenous (45 %) and isolipidic (8 %) experimental diets contained 17 % glucose, maltose, sucrose

Larval rearing techniques with suitable nursery feed should be prioritized for better survival and growth. We evaluate the potential use of Cyclops abyssorum divergens as a natural live food candidate instead of the costly traditional Artemia franciscana in the feeding strategy for Dicentrarchus labrax larvae. C. abyssorum divergens and A. franciscana were enriched with mixed valuable algae strains