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Repeats in mitochondrial and chloroplast genomes characterize the ecotypes of the Oryza Mol. Breed. (IF 2.149) Pub Date : 2021-01-13 Weilong Yang, Jianing Zou, Yajie Yu, Weixiong Long, Shaoqing Li
Mitochondria and chloroplast are very important organelles for organism, participating in basic life activity. Their genomes contain many repeats which can lead to a variation of genome structure. Oryza is an important genus for human beings’ nutrition. Several mitochondrial and chloroplast genomes of Oryza have been sequenced, which help us to insight the distribution and evolution of the repeats
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Identification of genetic loci and candidate genes related to β-glucan content in barley grain by genome-wide association study in International Barley Core Selected Collection Mol. Breed. (IF 2.149) Pub Date : 2021-01-13 La Geng, Mengdi Li, Shanggeng Xie, Dezhi Wu, Lingzhen Ye, Guoping Zhang
β-glucan is an important trait to be improved in barley breeding programs, as it greatly affects the quality of the end products when barley grains are used as raw material of feed or malt production or consumed as food for human. Although the genes associated with β-glucan synthesis have been identified, genetic regulation of β-glucan accumulation in barley grains is still completely unclear. In this
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Characterization of near-isogenic lines confirmed QTL and revealed candidate genes for plant height and yield-related traits in common wheat Mol. Breed. (IF 2.149) Pub Date : 2021-01-12 Yunxiao Zhang, Hui Liu, Guijun Yan
Plant height (PH) is closely associated with yield-related traits and environmental adaptation. Seven pairs of near-isogenic lines (NILs) targeting four QTL on 3AL, 4BL, 4AS, and 7AL wheat chromosome arms were assessed for PH and four yield-related traits including yield per plant (Y/P), grain number per spike (G/S), thousand kernel weight (TKW), and biomass per plant (B/P). Significant differences
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Investigating genetic relationship of Brassica juncea with B. nigra via virtual allopolyploidy and hexaploidy strategy Mol. Breed. (IF 2.149) Pub Date : 2021-01-12 Jin Liu, Kusum Rana, John McKay, Zhiyong Xiong, Fengqun Yu, Jiaqin Mei, Wei Qian
Brassica juncea is an important economic crop of the world; however, the narrow genetic base of this crop has tremendously decreased its crop productivity. As an ancestral species of B. juncea, B. nigra is of great importance in widening the genetic diversity of B. juncea. In the present study, 42 SSR markers were employed to screen the genetic diversity among 83 B. nigra, 16 B. juncea, and other Brassica
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Molecular-assisted breeding for soybean with high oleic/low linolenic acid and elevated vitamin E in the seed oil Mol. Breed. (IF 2.149) Pub Date : 2021-01-07 Katherine Hagely, Anji Reddy Konda, Jeong-Hwa Kim, Edgar B. Cahoon, Kristin Bilyeu
The uses of vegetable oils are determined by functional properties arising from their chemical composition. Soybean oil was previously used in margarines and baked foods after partial hydrogenation to achieve heat and oxidative stability. This process, however, generates trans fats that are now excluded from food use because of cardiovascular health risks. Also present in soybean oil are the anti-oxidant
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Maize kernel development Mol. Breed. (IF 2.149) Pub Date : 2021-01-03 Dawei Dai, Zeyang Ma, Rentao Song
Maize (Zea mays) is a leading cereal crop in the world. The maize kernel is the storage organ and the harvest portion of this crop and is closely related to its yield and quality. The development of maize kernel is initiated by the double fertilization event, leading to the formation of a diploid embryo and a triploid endosperm. The embryo and endosperm are then undergone independent developmental
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Elucidation of genomic organizations of transgenic soybean plants through de novo genome assembly with short paired-end reads Mol. Breed. (IF 2.149) Pub Date : 2020-12-31 Myung-Shin Kim, Hojin Jo, Ji Hong Kim, Dong Nyuk Bae, In-Soon Pack, Chang-Gi Kim, Tackmin Kwon, Jaesung Nam, Young-Soo Chung, Soon-Chun Jeong
Elucidation of the genomic organizations of transgene insertion sites is essential for the genetic studies of transgenic plants. Herein, we establish an analysis pipeline that identifies the transgene insertion sites as well as the presence of vector backbones, through de novo genome assembly with high-throughput sequencing data in two transgenic soybean lines, AtYUCCA6-#5 and 35S-UGT72E3/2-#7. Sequencing
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Comparative metabolomics analysis reveals the variations of eating quality among three high-quality rice cultivars Mol. Breed. (IF 2.149) Pub Date : 2020-12-10 Rong Gong, Daoqiang Huang, Yibo Chen, Hong Li, Zhidong Wang, Degui Zhou, Lei Zhao, Yangyang Pan, Yuxiao Chang, Yong Xiang, Chongrong Wang, Shaochuan Zhou
Good eating quality is a highly desirable trait of rice which determines its commercial value and market share. However, the molecular basis of this trait remains largely unknown. Here, three high-quality conventional rice cultivars, including two superior eating quality cultivars Meixiangzhan-2 (MXZ) and Xiangyaxiangzhan (XYXZ), and one ordinary eating quality cultivar Huanghuazhan (HHZ), were analyzed
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Waterlogging tolerance in maize: genetic and molecular basis Mol. Breed. (IF 2.149) Pub Date : 2020-12-09 Kun Liang, Kaiyuan Tang, Tian Fang, Fazhan Qiu
Maize (Zea mays L.) is an important crop around the world, but its growth and production can be severely limited by waterlogging. In response to waterlogging stress, elaborate regulatory mechanisms have evolved in maize under both natural and artificial selection. Some research efforts involving genetic, genomic, and molecular strategies have focused on waterlogging stress biology in maize. Waterlogging
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Ghd7 is a negative regulator of zinc concentration in brown rice Mol. Breed. (IF 2.149) Pub Date : 2020-12-03 Mufid Alam, Yingnanjun Chen, Pingbo Li, Guangming Lou, Hao Zhou, Lei Wang, Rongjia Liu, Pingli Chen, Yin Zhou, Guanjun Gao, Dewei Tai, Xiaozhong Zhang, Yuqing He
Zinc (Zn) is an essential micronutrient for human, and improving Zn concentration in rice could be a simple and effective way to solve insufficient Zn uptake from daily diet. In this study, a recombinant inbred line (RIL) population was developed from a commercial hybrid combination Wandao153, and subjected to quantitative trait loci (QTL) detection for Zn concentration in brown rice and heading date
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Genetic analysis of rice seed recovery under low-temperature conditions using a new CSSL population with a high-density genetic map in rice Mol. Breed. (IF 2.149) Pub Date : 2020-11-29 Guangliang Wu, Haodong Deng, Meixia Yu, Yicong Cai, Dahu Zhou, Jingai Tan, Jianfeng Yu, Xin Luo, Shan Tong, Peng Wang, Xiangyu Zhang, Caijin Li, Cuijuan Li, Yanning Wang, Qin Cheng, Haohua He, Jianmin Bian
Developing new population types based on interspecific introgressions has been suggested to facilitate the discovery of novel allelic sources for traits of agronomic importance. In this study, a chromosome segment substitution line (CSSL) rice population carrying contiguous chromosomal segments of japonica Nipponbare in the genetic background of indica 9311 was developed. Meanwhile, a high-density
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Abscisic acid enhances tolerance to spring freeze stress and regulates the expression of ascorbate–glutathione biosynthesis-related genes and stress-responsive genes in common wheat Mol. Breed. (IF 2.149) Pub Date : 2020-11-19 Ning Zhang, Linjie Li, Lingran Zhang, Jia Li, Yuhui Fang, Lei Zhao, Yan Ren, Feng Chen
The present study showed that exogenous abscisic acid (ABA) pre-treatment remarkably increases the freeze tolerance of common wheat (Triticum aestivum L.) seedlings at the anther connective tissue formation phase (ACFP) and tetrad developmental phase. After 3-day freeze stress, ABA pre-treatment significantly increases the content of soluble sugar, soluble protein, and proline, activities of superoxide
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Favorable allele mining and breeding utilization of ALK in rice Mol. Breed. (IF 2.149) Pub Date : 2020-11-18 Ling Zheng, Pin Liu, Shangxing Zhang, Jialian Li, Yaseen Muhammad, Yifan Yun, Li Hu, Fengying Xue, Yangkai Wang, Hua Yuan, Weilan Chen, Peng Qin, Bingtian Ma, Shigui Li, Bin Tu, Yuping Wang
ALK is the key gene of controlling rice gelatinization temperature (GT), and at least three ALK alleles are thought to be responsible for the diversity of GT among rice cultivars. But whether the sequence polymorphism of the promoter region also associated with GT is not clearly studied. In this study, we analyzed sequence variation in the promoter region of ALK and mainly two types of promoter were
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Host reaction of tomato varieties and applicability of Sm -linked DNA markers to Stemphylium lycopersici Mol. Breed. (IF 2.149) Pub Date : 2020-11-18 Jiyeon Park, Seungahn Kwon, Girim Park, Siyoung Lee, Byoung Il Je, Younghoon Park
Gray leaf spot (GLS) disease, caused by the Stemphylium (Sm) fungus, affects a wide range of horticultural crops including tomato. In this study, the South Korean Sm isolate (PNU-SM01) was identified, and the applicability of Sm-resistance gene (Sm)-linked DNA markers in marker-assisted selection was validated. ITS/gpd sequencing and morphological observation identified the isolate as S. lycopersici
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A candidate gene for the determination of rice resistant to rice false smut Mol. Breed. (IF 2.149) Pub Date : 2020-11-17 Jiehua Qiu, Feifei Lu, Hong Wang, Junhui Xie, Congcong Wang, Zhiquan Liu, Shuai Meng, Huangbin Shi, Xihong Shen, Yanjun Kou
In recent years, rice false smut (RFS) has grown from being a minor to a major disease affecting rice productivity. The genetic basis of the host’s defense against RFS is currently under-researched. In this study, a rice variety Nanjing11 with stable resistance to RFS from years under naturally infected field condition was identified. The genetic analysis showed that the RFS resistance of Nanjing11
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Allelic diversity study of functional genes in East Africa bread wheat highlights opportunities for genetic improvement Mol. Breed. (IF 2.149) Pub Date : 2020-11-10 Mercy Wamalwa, Zerihun Tadesse, Lucy Muthui, Nasser Yao, Habtemariam Zegeye, Mandeep Randhawa, Ruth Wanyera, Cristobal Uauy, Oluwaseyi Shorinola
Wheat (Triticum aestivum L.) is a major staple crop in East Africa (EA) providing 9% and 10% of daily calories and protein intake, respectively. However, EA countries depend on import to meet 55% of their domestic wheat supplies due to increasing demands and low domestic yields. To determine the beneficial gene pool currently exploited for wheat improvement in EA, we examined the allelic diversity
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Genome-wide association for β-glucan content, population structure, and linkage disequilibrium in elite oat germplasm adapted to subtropical environments Mol. Breed. (IF 2.149) Pub Date : 2020-11-02 Cristiano M. Zimmer, Ian G. McNish, Kathy Esvelt Klos, Tatiana Oro, Klever M. A. Arruda, Luiz C. Gutkoski, Marcelo T. Pacheco, Kevin P. Smith, Luiz C. Federizzi
High β-glucan content is one of the main goals of oat breeding programs worldwide. However, the genomic regions and genes controlling β-glucan content in oats are not fully understood. In this sense, the objectives of this study were as follows: (i) to characterize structure and linkage disequilibrium (LD) in a panel of oat germplasm adapted to subtropical environments; and (ii) to identify genomic
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Retraction Note: Cloning and characterization of TaPUB4 , a U-box gene from common wheat ( Triticum aestivum L.) involved in regulation of pollen development by influencing sucrose-starch pathway in anther Mol. Breed. (IF 2.149) Pub Date : 2020-10-17 Weidong Zhang, Liang Wang, Yongzhen Wang, Yue Wang, Qingrong Gao
The Editor-in-Chief has retracted this article [1] at the request of the first and last author. Following publication, the first and last author have stated that the quantitative real-time PCR (qPCR) analysis was outsourced to a third party.
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Development and evaluation of improved lines based on an elite rice variety 9311 for overcoming hybrid sterility in rice Mol. Breed. (IF 2.149) Pub Date : 2020-10-17 Xiaojuan Ma, Xin Wei, Guanjun Gao, Haichao Jiang
Hybrid sterility is a major obstacle to the development of superior inter-subspecific hybrids between indica and japonica subspecies of Asian-cultivated rice. To overcome hybrid sterility, we introduced four japonica alleles (S-j) at Sa, Sc, f5, and S32 loci from Nipponbare into 9311 through marker-assisted backcross breeding, and four improved lines were obtained. Three wide-compatibility lines 9311(S5-n)
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Loci and candidate gene identification for soybean resistance to Phytophthora root rot race 1 in combination with association and linkage mapping Mol. Breed. (IF 2.149) Pub Date : 2020-10-15 Xue Zhao, Dongfang Bao, Wei Wang, Chanjuan Zhang, Yan Jing, Haipeng Jiang, Lijuan Qiu, Wenbin Li, Yingpeng Han
As the second most destructive disease in soybean (Glycine max (L.) Merr), Phytophthora root or stem-rot (PRR) of soybean, which was caused by Phytophthora sojae (P. sojae), could lead to a 10–40% or complete yield loss annually. Researches were needed to perform to identify the P. sojae-resistant germplasm and to better understand the genetic basis of P. sojae resistance in soybean. A total of 225
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Identification of major QTLs for flowering and maturity in soybean by genotyping-by-sequencing analysis Mol. Breed. (IF 2.149) Pub Date : 2020-10-13 Lingshuang Wang, Chao Fang, Jun Liu, Ting Zhang, Kun Kou, Tong Su, Shichen Li, Liyu Chen, Qun Cheng, Lidong Dong, Fanjiang Kong, Baohui Liu, Sijia Lu
As soybean is highly sensitive to photoperiod, and the cultivation area of soybean is restricted to a narrow latitude region. The adaptability of soybean growth across wide latitude areas is owing to the rich variations of genes conditioning the flowering time and maturity. Identification of new quantitative trait loci (QTLs) and validation of the potential genes are beneficial to clarify the complex
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A cotton germin-like protein GbGLP2 controls fiber length via regulating genes involved in secondary cell wall synthesis Mol. Breed. (IF 2.149) Pub Date : 2020-10-06 Mengling Sun, Zhengxiu Ye, Jiafu Tan, Sheng Chen, Xianlong Zhang, Lili Tu
Cotton is not only a natural fiber crop, but also an important economic crop in China. The cultivation of cotton with longer, stronger, and finer fiber has been the goal of cotton breeders. Germin-like proteins (GLPs) is a group of protein family with cupin domain, which mainly acts as an enzyme. However, the function of GLPs involved in regulating cotton fiber development is rarely understood. Here
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Overexpression of the Raphanus sativus RsMYB1 using the flower-specific promoter (InMYB1) enhances anthocyanin accumulation in flowers of transgenic Petunia and their hybrids Mol. Breed. (IF 2.149) Pub Date : 2020-09-29 Aung Htay Naing, Hyun Hee Kang, Hui Yeong Jeong, May Thu Soe, Junping Xu, Chang Kil Kim
The generation of transgenic floricultural crops for flower color modification requires a transgene that enhances anthocyanin accumulation and a flower-specific promoter with stable expression throughout flowering stage. Here, the anthocyanin regulatory R2R3-MYB transcription factor (TF) RsMYB1 was placed under the control of the stable flower-specific promoter (InMYB1) to enhance anthocyanin accumulation
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An efficient Agrobacterium -mediated transformation method using hypocotyl as explants for Brassica napus Mol. Breed. (IF 2.149) Pub Date : 2020-09-28 Cheng Dai, Yuqing Li, Long Li, Zhuolin Du, Shengli Lin, Xia Tian, Sijia Li, Bao Yang, Wei Yao, Jing Wang, Liang Guo, Shaoping Lu
Rapeseed (Brassica napus) is an important oil crop that supplies a considerable amount of global vegetable oil production. Genetic transformation system is important to gene functional analysis and molecular breeding. Here, an efficient Agrobacterium-mediated transformation protocol using hypocotyl of rapeseed as explants is described. To develop this protocol, we compared several essential factors
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Development of InDel markers of Bph3 and pyramiding of four brown planthopper resistance genes into an elite rice variety Mol. Breed. (IF 2.149) Pub Date : 2020-09-25 Long He, Ling Zou, Qiuli Huang, Xichen Sheng, Weiren Wu, Jie Hu
Brown planthopper (BPH) is one of the most destructive pests in rice. Breeding resistant cultivars is the most effective strategy to control BPH. Bph3 is a broad-spectrum and durable BPH resistance locus spanning ~ 50 kb on the short arm of rice chromosome 4. Using the genomic variation data of 4726 rice varieties from public database, we developed 8 InDel markers within the Bph3 locus. By analyzing
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QTL mapping of resistance to Gibberella ear rot in maize Mol. Breed. (IF 2.149) Pub Date : 2020-09-25 Jing Wen, Yanqi Shen, Yuexian Xing, Ziyu Wang, Siping Han, Shijie Li, Chunming Yang, Dongyun Hao, Yan Zhang
Gibberella ear rot (GER), caused by the fungal pathogen Fusarium graminearum, is becoming one of the most prominent pathogens responsible for ear rot in maize. In this study three F2 populations, F2-C, F2-D, and F2-J, and their corresponding F2:3 families, were constructed by crossing three highly GER-resistant inbred lines—Cheng351, Dan598, and JiV203—with the susceptible line ZW18. We used this cross
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Pyramiding seed dormancy genes to improve resistance of semi-dwarf varieties to pre-harvest sprouting in rice Mol. Breed. (IF 2.149) Pub Date : 2020-09-17 Junwei Wang, Ugur Korkmaz, Min Guo, Wirat Pipatpongpinyo, Xing-You Gu
The quantitative trait locus SD1-2 for seed dormancy (SD) is identical to the semi-dawrf1 (sd1) gene prevailing in high-yield varieties of rice. However, sd1 alone appeared not sufficient to overcome the preharvest sprouting (PHS) problem in crop (or hybrid seed) production. This research aimed to address some genetic issues that may impact pyramiding of SD genes to improve semi-dwarf varieties for
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Location and mapping of the NCLB resistance genes in maize by bulked segregant analysis (BSA) using whole genome re-sequencing Mol. Breed. (IF 2.149) Pub Date : 2020-09-16 Chunlei Li, Fenglou Ling, Guihua Su, Weifeng Sun, Hongshuang Liu, Yichen Su, Xin Qi
Northern corn leaf blight (NCLB) is one of the main diseases of maize, which greatly reduces production and causes millions of dollars in losses worldwide annually. Identification of NCLB resistance genes in maize plays an important role in the crop disease resistance breeding. In this study, a BC1F1 population with 1290 individuals was constructed using high resistance line CML493 and high susceptible
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QTL mapping for resistance to ear rot caused by Fusarium graminearum using an IBM Syn10 DH population in maize Mol. Breed. (IF 2.149) Pub Date : 2020-09-10 Guangsheng Yuan, Benfang Chen, Hua Peng, Qi Zheng, Yucui Li, Kui Xiang, Li Liu, Chaoying Zou, Haijian Lin, Haiping Ding, Guangtang Pan, Zhiming Zhang
Gibberella ear rot (GER) caused by Fusarium graminearum (teleomorph Gibberella zeae), is one of the most devastating diseases in maize that not only severely reduces grain yield but also contaminates the kernels with potent mycotoxins which is harmful to livestock and humans. Identification of stable quantitative trait loci (QTL) and candidate genes conferring resistance is a promising strategy in
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A rice GDSL esterase/lipase protein (GELP) is required for anther and pollen development Mol. Breed. (IF 2.149) Pub Date : 2020-09-08 Guoqiang Yuan, Ting Zou, Xu Zhang, Miaomiao Liu, Tingting Luo, Zhiyuan He, Yang Tao, Dan Zhou, Hongfeng Zhao, Sijin Liu, Rui Liu, Menglin Zhou, Jun Zhu, Yueyang Liang, Qiming Deng, Shiquan Wang, Aiping Zheng, Aijun Wang, Huainian Liu, Lingxia Wang, Ping Li, Shuangcheng Li
Male reproductive development is vital for crop seed production. However, the mechanism underlying remains largely unknown. Here, we isolated a male sterile mutant osgelp34-1 in rice. Cytological analysis indicated that the mutant exhibited delayed degradation of anther wall layers and abnormal pollen development, which resulted in the production of shrunk and non-viable pollen grains. MutMap and CRISPR/Cas9
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Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di- and tetraploid levels Mol. Breed. (IF 2.149) Pub Date : 2020-09-05 Emil Stefańczyk, Jarosław Plich, Marta Janiszewska, Paulina Smyda-Dajmund, Sylwester Sobkowiak, Jadwiga Śliwka
Late blight is a disease with the biggest economic impact on potato cultivation worldwide. Pyramiding of the resistance genes originating from potato wild relatives is a breeding strategy that has a potential to produce potato cultivars durably resistant to late blight. Growing such cultivars would allow limiting the intensive chemical control of the disease. The goal of this work was to transfer the
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Genetic and epistatic effects for grain quality and yield of three grain-size QTLs identified in brewing rice ( Oryza sativa L.). Mol. Breed. (IF 2.149) Pub Date : 2020-09-03 Satoshi Okada, Ken Iijima, Kiyosumi Hori, Masanori Yamasaki
Rice (Oryza sativa L.) in Japan is not only a food staple but also an important material for the Japanese alcoholic beverage, sake. The grain used in sake brewing has different characters from the cooking rice grain, including a large grain size and high white-core expression rate (WCE). Because large-sized grains often have a heavy grain weight and higher yield, this trait is also important for cooking
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Molecular breeding of a high oleic acid soybean line by integrating natural variations Mol. Breed. (IF 2.149) Pub Date : 2020-08-31 Haiyang Nan, Sijia Lu, Chao Fang, Zhihong Hou, Cen Yang, Qian Zhang, Baohui Liu, Fanjiang Kong
Soybean is an important crop used for oil production. Alterations of the fatty acids, especially increased oleic acid content, can improve the nutritional quality and oxidative stability of soybean oil. During seed development, two genes, encoding FAD2-1A and FAD2-1B, are mainly responsible for the transformation from oleic acid to linoleic acid, and the combination of fad2-1a and fad2-1b is the key
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Fruit size and firmness QTL alleles of breeding interest identified in a sweet cherry ‘Ambrunés’ × ‘Sweetheart’ population Mol. Breed. (IF 2.149) Pub Date : 2020-08-26 Alejandro Calle, Francisco Balas, Lichun Cai, Amy Iezzoni, Margarita López-Corrales, Manuel J. Serradilla, Ana Wünsch
The Spanish local cultivar ‘Ambrunés’ stands out due to its high organoleptic quality and fruit firmness. These characteristics make it an important parent for breeding cherries with excellent fresh and post-harvest quality. In this work, an F1 sweet cherry population (n = 140) from ‘Ambrunés’ × ‘Sweetheart’ was phenotyped for 2 years for fruit diameter, weight and firmness and genotyped with the RosBREED
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Effects of waterlogging stress on early seedling development and transcriptomic responses in Brassica napus Mol. Breed. (IF 2.149) Pub Date : 2020-08-24 Yiyi Guo, Juan Chen, Liuhui Kuang, Nianhong Wang, Guoping Zhang, Lixi Jiang, Dezhi Wu
In this study, we performed a transcriptomic analysis to investigate genome-wide gene profiles of a rapeseed (Brassica napus) cultivar Zhongshuang 11 (ZS11) in response to waterlogging stress at germination stage. The root and shoot development of ZS11 was dramatically inhibited by waterlogging stress. As a result, 7472 downregulated DEGs (differentially expressed genes) and 3869 upregulated DEGs were
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Re-evaluation of the rice ‘Green Revolution’ gene: the weak allele SD1-EQ from japonica rice may be beneficial for super indica rice breeding in the post-Green Revolution era Mol. Breed. (IF 2.149) Pub Date : 2020-08-21 Yilan Yu, Xiaojie Hu, Yuxing Zhu, Donghai Mao
The Green Revolution of the 1960s introduced semi-dwarf cultivars of rice that had reduced lodging and increased harvest indexes. The mutant allele of Semi Dwarf 1 (SD1) is the gene underlying the Green Revolution advances in rice yield. Since that time, most of the commercial semi-dwarf cultivars carry a mutation in SD1. Unlike japonica rice cultivars containing weak functional SD1 alleles, indica
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Mining of candidate genes for nitrogen use efficiency in maize based on genome-wide association study Mol. Breed. (IF 2.149) Pub Date : 2020-08-17 Kunhui He, Shutu Xu, Xinghua Zhang, Yanan Li, Liguo Chang, Yahui Wang, Yaqin Shi, Tingting Cui, Yuan Dong, Tianru Lan, Xueyan Liu, Yutang Du, Renhe Zhang, Jianchao Liu, Jiquan Xue
Improving the nitrogen use efficiency (NUE) may considerably increase maize yield and decrease the use of nitrogen (N) fertilizer. But the genetic basis of NUE in maize is still poorly understood. In this study, an association panel of 139 maize inbred lines genotyped with 50,790 single nucleotide polymorphism (SNP) was used to dissect the genetic basis of NUE-related traits by genome-wide association
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Fine mapping of qSdr9 , a novel locus for seed dormancy (SD) in weedy rice, and development of NILs with a strong SD allele Mol. Breed. (IF 2.149) Pub Date : 2020-08-15 Thanhliem Nguyen, Kai Fu, Changling Mou, Jiangfeng Yu, Xingjie Zhu, Yunshuai Huang, Chunlei Zhou, Qixian Hao, Fulin Zhang, Weihan Song, Ping Wang, Yaping Chen, Tengfei Ma, Yunlu Tian, Shijia Liu, Ling Jiang, Jianmin Wan
Lack of seed dormancy, a major cause of pre-harvest sprouting in rice and other cereal crops, causes significant reductions in grain yield and quality. Weedy rice is often strongly dormant; therefore, quantitative trait loci (QTLs) related to seed dormancy in weedy rice “Ludao” could be useful for improving pre-harvest sprouting resistance in the important japonica variety “Ninggeng 4.” To identify
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QTL mapping of resistance to Ug99 and other stem rust pathogen races in bread wheat Mol. Breed. (IF 2.149) Pub Date : 2020-08-15 Silvina Baraibar, Richard García, Paula Silva, Bettina Lado, Ariel Castro, Lucía Gutiérrez, Monika Kavanová, Martín Quincke, Sridhar Bhavani, Mandeep S. Randhawa, Silvia Germán
Most wheat cultivars planted worldwide are susceptible to the stem rust Ug99 race group. To prepare for the potential spread of these races into South America, we aimed to identify genomic regions responsible for resistance to Ug99 race group in germplasm adapted to South America. Two RIL populations from a cross between a stem rust susceptible parent “Baguette 13” and resistant local parents “INIA
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Genetic variation associated with the shoot biomass of upland cotton seedlings under contrasting phosphate supplies Mol. Breed. (IF 2.149) Pub Date : 2020-08-04 Qishen Gu, Huifeng Ke, Zhengwen Sun, Liqiang Wu, Guiyin Zhang, Yan Zhang, Zhikun Li, Xingfen Wang, Zhiying Ma
As over-fertilization leads to environmental concerns, selecting cultivars that can produce high yields on phosphorus (P)-deficient soils and understanding the genetic basis underlying P-starved tolerance have become crucially important. In this study, we evaluated the variation of ten P deficiency-related traits at the seedling stage of 419 core accessions in two different environments. The results
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Genome-wide identification and comparative analysis of resistance genes in Brassica juncea Mol. Breed. (IF 2.149) Pub Date : 2020-08-03 Fabian Inturrisi, Philipp E. Bayer, Hua Yang, Soodeh Tirnaz, David Edwards, Jacqueline Batley
Disease resistance in Brassica juncea has been shown against several diseases including blackleg, white rust, hypocotyl rot, turnip mosaic virus, leaf blight, black rot, and Alternaria blight. The identification of disease resistance genes is important to develop elite cultivars, which can survive exposure to these diseases. The largest family of resistance genes are nucleotide-binding site leucine-rich
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Exploration and selection of elite Sd1 alleles for rice design breeding Mol. Breed. (IF 2.149) Pub Date : 2020-08-03 Lin Zhang, Zhong Bian, Bin Ma, Xiaoyuan Li, Yiting Zou, Dong Xie, Jiyun Liu, Yonghong Ren, Changquan Zhang, Junmin Wang, Shenghai Ye, Yiwen Deng, Qun Li, Xiaoming Zhang, Zuhua He, Qiaoquan Liu
Plant height is the important determinant for the yield formation of rice, and selecting of an optimal plant height is necessary during rice breeding to achieve ideal biomass and lodging resistance. In this study, allele variation of Sd1, the well-known “green revolution” gene, was clarified as the major factor for plant height segregation in the breeding populations from an indica and japonica cross
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Correction to: Characterization of three soybean landraces resistant to Asian soybean rust disease Mol. Breed. (IF 2.149) Pub Date : 2020-08-01 Luciano Nobuhiro Aoyagi, Yukie Muraki, Naoki Yamanaka
The original article unfortunately contains mistake.
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Dominance complementation of Hd1 and Ghd8 contributes to extremely late flowering in two rice hybrids Mol. Breed. (IF 2.149) Pub Date : 2020-07-31 Jie Liu, Junyi Gong, Xin Wei, Shihua Yang, Xuehui Huang, Can Li, Xiaoyi Zhou
A rice hybrid is the product of a cross between two genetically distinct inbred lines. Owing to yield heterosis in rice hybrids, hybrid rice breeding has been developed and widely used in agriculture. In some cases, the hybrid F1 lines show extremely late flowering. However, the genetic basis of the phenomenon is not very clear. In this study, two hybrids were found to have heading date of ~ 130 days
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Loci and alleles for submergence responses revealed by GWAS and transcriptional analysis in rice Mol. Breed. (IF 2.149) Pub Date : 2020-07-29 Hongsheng Gao, Chao Zhang, Huiying He, Tianjiao Liu, Bin Zhang, Hai Lin, Xiaoxia Li, Zhaoran Wei, Qiaoling Yuan, Quan Wang, Chunyan Yu, Lianguang Shang
The low seedling rate of rice caused by submergence in the process of rice direct seeding has always been an important factor limiting the popularization of rice direct seeding technology. Improving the tolerance of rice to submergence stress will benefit the production of rice and the promotion of direct seeding technology. In this study, we determined the submergence coleoptile length (SCL), submergence
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QTLs detection and pyramiding for stigma exsertion rate in wild rice species by using the single-segment substitution lines Mol. Breed. (IF 2.149) Pub Date : 2020-07-28 Tuo Zou, Hanwei Zhao, Xiaohui Li, Mingmin Zheng, Shaodi Zhang, Lingling Sun, Ning He, Xiaoping Pan, Ziqiang Liu, Xuelin Fu
Seed yield is one of the main limitations in hybrid rice production. The improvement of stigma exsertion rate (SER) of male sterility lines is essential for the higher seed yield in hybrid rice. Wild rice species are valuable germplasms with exserted stigma. It is worthy of mining-related genes for SER in wild rice species, as well as probing the way to use the beneficial genes in rice breeding. In
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Population genomic and genome-wide association analysis of lignin content in a global collection of 206 forage sorghum accessions Mol. Breed. (IF 2.149) Pub Date : 2020-07-23 Hao Niu, Junai Ping, Yubin Wang, Xin Lv, Huiming Li, Fuyao Zhang, Jianqiang Chu, Yuanhuai Han
Forage sorghum (Sorghum bicolor (L.) Moench) is a wildly cultivated C4 cereal crop in many geographical regions and differs among germplasms in a number of important physiological traits. Lignin is a complex heteropolymer found in plant cell walls that adversely affects economic and environmental benefits of the crop. To understand the genetic basis, we re-sequenced the genomes of 206 sorghum accessions
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Overexpression of ZmDWF4 improves major agronomic traits and enhances yield in maize Mol. Breed. (IF 2.149) Pub Date : 2020-07-17 Na Liu, Ya Jie Zhao, Jia Wen Wu, Yi Ming Wei, Ru Chang Ren, Jie Zang, Wen Ting Zhang, Lin Zhang, Qiang Shen, Xian Sheng Zhang, Xiang Yu Zhao
Brassinosteroids (BRs) are a class of plant-specific steroid hormones and play an essential role in plant growth and development. The DWARF4 (DWF4) gene encodes a C-22 hydroxylase, which is a rate-limiting enzyme in BR biosynthesis pathway. Here, the DWF4 (ZmDWF4) in maize (Zea mays L.), the ortholog of Arabidopsis DWF4 (CYP90B1), was transformed into elite inbred Q319 and driven maize ubiquitin promoter
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Correction to: Toward validation of QTLs associated with pod and seed size in common bean using two nested recombinant inbred line populations Mol. Breed. (IF 2.149) Pub Date : 2020-07-17 Ester Murube, Ana Campa, Qijian Song, Phillip McClean, Juan Jose Ferreira
Unfortunately, in the final version of this article the original Table 6 was not included in the proofing step and the Table 5 was duplicated.
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Genome-wide association study for soybean mosaic virus SC3 resistance in soybean Mol. Breed. (IF 2.149) Pub Date : 2020-07-15 Zhijun Che, Honglang Yan, Hailun Liu, Hui Yang, Haiping Du, Yuming Yang, Baohui Liu, Deyue Yu
Soybean mosaic virus (SMV) threatens soybean (Glycine max (L.) Merr.) production and seed quality worldwide. Host plant resistance is the most environmentally friendly and economically effective method to deduce the damages of SMV. However, the use of limited resistant loci in SMV management will be conquered due to SMV strain shifts caused by evolutionary arms races. Thus, exploration of novel germplasms
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A major and stable QTL controlling wheat thousand grain weight: identification, characterization, and CAPS marker development Mol. Breed. (IF 2.149) Pub Date : 2020-07-15 Xixian Duan, Haixia Yu, Wujun Ma, Jiaqiang Sun, Yun Zhao, Rongchang Yang, Tangyuan Ning, Qingfang Li, Qianqian Liu, Tingting Guo, Min Yan, Jichun Tian, Jiansheng Chen
Thousand grain weight (TGW) is one of the three crucial determinants of wheat (Triticum aestivum) grain yield. The identification of functional genes and development of closely linked markers are urgently needed for wheat yield improvements. In this study, quantitative trait locus (QTL) mapping for TGW was carried out using one RIL population in four environments. A total of 8 QTL controlling TGW were
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Fine mapping and candidate gene analysis of qKW7b , a major QTL for kernel width in maize Mol. Breed. (IF 2.149) Pub Date : 2020-07-06 Bin Tang, Yongxiang Li, Zhisheng Mu, Lin Chen, Huanle Guo, Zhihui Chen, Chunhui Li, Xuyang Liu, Dengfeng Zhang, Yunsu Shi, Yu Li, Tianyu Wang
Kernel width (KW) is a key determinant of kernel weight and an important selection trait during the maize domestication and improvement. KW is a complex quantitative trait that is controlled by many quantitative trait loci (QTL) with minor effects, but the underlying genes for KW using natural variation remain rarely reported in maize. In this study, using advanced backcross populations from the cross
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Functional analysis of Medicago -derived pathogen-induced gene promoters for usage in transgenic alfalfa Mol. Breed. (IF 2.149) Pub Date : 2020-06-26 Andrew E. Sathoff, Melinda R. Dornbusch, Susan S. Miller, Deborah A. Samac
Three gene promoters from the model legume species Medicago truncatula and Medicago sativa (alfalfa) pathogenesis-related (PR) proteins (MtPR5, MtPR10, and MsPR10) were isolated and investigated using in silico and in situ approaches. For the functional analysis of these promoters, plant transformation vectors linking promoter sequences with the β-glucuronidase (GUS) reporter gene were constructed
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A naturally occurring insertion in BrEMS1 confers genic male sterility in turnip ( Brassica rapa ssp. rapa ) Mol. Breed. (IF 2.149) Pub Date : 2020-06-23 Kenji Komatsu, Miho Ozeki, Keisuke Tanaka, Rumi Ohtake, Yuki Mitsui, Kenji Wakui
To identify genes associated with genic male sterility (GMS) that are useful for hybrid breeding in Brassica crops, we analyzed the “ms77B” strain, which is a spontaneous turnip GMS mutant derived from a cv. 77B population. Previously, we reported that the GMS phenotype of ms77B is caused by a single gene with sterility as the recessive trait. To identify the causative gene of GMS in ms77B, we performed
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Genetic dissection of the interactions between semi-dwarfing genes sdw1 and ari-e and their effects on agronomic traits in a barley MAGIC population Mol. Breed. (IF 2.149) Pub Date : 2020-06-23 Viet Hoang Dang, Camilla Beate Hill, Xiao-Qi Zhang, Tefera Tolera Angessa, Lee-Anne McFawn, Chengdao Li
The introduction of semi-dwarf cereal crop varieties in the 1960s enabled extensive increases in global food production. Semi-dwarf barley cultivars have shorter stature and improved stress resistance, grain yield and quality than tall varieties. The major semi-dwarf genes, sdw1 and ari-e, have been widely used in barley breeding programmes worldwide. In this study, we identified a novel sdw1 allele
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A novel aquaporin gene MaSIP2-1 confers tolerance to drought and cold stresses in transgenic banana Mol. Breed. (IF 2.149) Pub Date : 2020-06-20 Yi Xu, Jingyang Li, Shun Song, Juhua Liu, Xiaowan Hou, Yujia Li, Anbang Wang, Dongmei Huang, Jiashui Wang, Zhuye Xu, Dongfang Zhao, Wei Hu, Biyu Xu, Zhiqiang Jin
Plant aquaporins (AQPs) are subdivided into eight groups: PIPs, NIPs, GIPs, HIPs, XIPs, LIPs, TIPs, and SIPs. However, the genetic function and physiological mechanisms of the SIP group in abiotic stress response in plants remains unknown. In this study, we cloned and characterized a banana SIP group AQP gene, designated MaSIP2-1. MaSIP2-1 showed upregulation after osmotic, salt, and cold treatments
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Identification of quantitative trait loci underlying resistance and tolerance to the rice root-knot nematode, Meloidogyne graminicola , in Asian rice ( Oryza sativa ) Mol. Breed. (IF 2.149) Pub Date : 2020-06-18 Judith Galeng-Lawilao, B. P. Mallikarjuna Swamy, Tapas Kumer Hore, Arvind Kumar, Dirk De Waele
Meloidogyne graminicola is one of the most important plant-parasitic nematodes in rice. Breeding for natural resistance and tolerance is considered one of the most economical and sustainable approaches to control this damaging pathogen. Quantitative trait loci (QTL) mapping was carried out in a recombinant inbred line (RIL) population derived from a cross between the Asian rice genotypes IR78877-208-B-1-2
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Identification of a male sterile gene Ms in Brassica rapa L. Mol. Breed. (IF 2.149) Pub Date : 2020-06-16 Fengyan Shi, Zhiyong Liu, Nan Wang, Ying Zhao, Shiyao Dong, Jiaqi Zou, Hui Feng
Male sterile lines are important for hybrid seed production in Brassica rapa. In our previous study, we obtained a multiple-allele inherited male sterile line of Chinese cabbage. To identify the male sterile gene Ms in B. rapa, we used B. rapa L. var. chinensis (‘AB03’; MsMs) and B. rapa var. purpuraria (‘AB04’; MsfMsf) as parents to construct an F2 segregating population, and mapped the Ms gene on
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Identification of QTL and genes for pod number in soybean by linkage analysis and genome-wide association studies Mol. Breed. (IF 2.149) Pub Date : 2020-06-15 Jie Song, Xu Sun, Kaixin Zhang, Shulin Liu, Jiajing Wang, Chang Yang, Sitong Jiang, Mahfishan Siyal, Xiyu Li, Zhongying Qi, Yue Wang, Xiaocui Tian, Yanlong Fang, Zhixi Tian, Wen-Xia Li, Hailong Ning
Pod number is an important component of yield in soybean (Glycine max (L.) Merr.), and pods are unevenly distributed in the upper, middle and lower parts of the plant. Here, linkage analysis combined with genome-wide association study (GWAS) was used to identify 20 pod number-related traits in four-way recombinant inbred lines (FW-RILs) derived from crosses between the four soybean varieties (Kenfeng
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qGLS1.02 , a novel major locus for resistance to gray leaf spot in maize Mol. Breed. (IF 2.149) Pub Date : 2020-06-15 Xiangling Lv, Maoxing Song, Zixiang Cheng, Xiaoyan Yang, Xiaocong Zhang, Zhiqiang Zhou, Chaoshu Zhang, Lei Zheng, Yuan Li, Kairong Lei, Hongwei Wang, Wanli Du, Fenghai Li, Xinhai Li, Jianfeng Weng
Gray leaf spot (GLS), caused by the fungal pathogen Cercospora zeae-maydis, is one of the most serious foliar diseases of maize worldwide. In the present study, a population of recombinant inbred lines (RILs) derived from a gray leaf spot–resistant inbred line Qi319 and a susceptible line Ye478 was used to map quantitative trait loci (QTL) for GLS resistance. For initial QTL analysis, genetic maps
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