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Anticancer Activities of Plant Secondary Metabolites: Rice Callus Suspension Culture as a New Paradigm Rice Sci. (IF 3.162) Pub Date : 2020-12-16 Wusirika Ramakrishna; Anuradha Kumari; Nafeesa Rahman; Pallavi Mandave
Plant natural products including alkaloids, polyphenols, terpenoids and flavonoids have been reported to exert anticancer activity by targeting various metabolic pathways. The biological pathways regulated by plant products can serve as novel drug targets. Plant natural compounds or their derivatives used for cancer treatment and some novel plant-based compounds which are used in clinical trials were
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Physiochemical Properties of Resistant Starch and Its Enhancement Approaches in Rice Rice Sci. (IF 3.162) Pub Date : 2020-12-16 Ding Yi; Wang Maike; Shen Yi; Shu Xiaoli; Wu Dianxing; Song Wenjian
With changes in food preferences and life styles, more and more attentions have been focused on healthier food. Nowadays, resistant starch (RS) which can resist digestion in the human intestine has been recognized and accepted. High RS diet shows great benefit for the human health, and breeding high RS rice variety is a great target for realizing dietary intervention. To provide guidance for RS improvement
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Flooding Tolerance in Rice: Focus on Mechanisms and Approaches Rice Sci. (IF 3.162) Pub Date : 2020-12-16 Debabrata Panda; Jijnasa Barik
Flooding is one of the most hazardous natural disasters and a major stress constraint to rice production throughout the world, which results in huge economic loss. Approximately one-fourth of the global rice crops (approximately 40 million hectares) are grown in rainfed lowland plots that are prone to seasonal flooding. A great progress has been made during last two decades in our understanding of
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Genetic and Geographic Patterns of Duplicate DPL Genes Causing Genetic Incompatibility Within Rice: Implications for Multiple Domestication Events in Rice Rice Sci. (IF 3.162) Pub Date : 2020-12-16 Xu Xun; Ge Song; Zhang Fumin
Strong genetic incompatibilities exist between two primary rice subspecies, indica and japonica. However, the wild ancestors of rice, O. nivara Sharma et Shastry and O. rufipogon Griff., are genetically compatible. How this genetic incompatibility became established has not been clearly elucidated. To provide insights into the process, we analyzed a pair of hybrid sterility genes in rice, DOPPELGANGER
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Visualizing Meiotic Chromosome Pairing and Segregation in Interspecific Hybrids of Rice by Genomic in situ Hybridization Rice Sci. (IF 3.162) Pub Date : 2020-12-16 Liu Mao-Sen; Tseng Shih-Hsuan; Chen Ting-Chu; Chung Mei-Chu
Meiotic disturbances in F1 hybrids and their progenies are still major problems in wide hybridization. To investigate the genome affinity reflected in chromosome pairing and segregation, we studied chromosome behaviors during meiosis in two interspecific F1 hybrids [O. minuta × O. australiensis (Om × Oa, BCE genome) and Oa × O. ridleyi (Or, EHJ genome)] by using both traditional staining methods and
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Identification of QTLs for Cadmium Tolerance During Seedling Stage and Validation of qCDSL1 in Rice Rice Sci. (IF 3.162) Pub Date : 2020-12-16 Ding Shilin; Liu Chaolei; Shang Lianguang; Yang Shenglong; Zhang Anpeng; Jiang Hongzhen; Ruan Banpu; Fang Guonan; Tian Biao; Ye Guoyou; Guo Longbiao; Qian Qian; Gao Zhenyu
Cadmium (Cd) is a non-essential toxic metal that is harmful to plants. To investigate the genetic mechanism of Cd tolerance in rice, quantitative trait loci (QTLs) associated with Cd tolerance at the seedling stage were analyzed using a recombinant inbred line (RIL) population derived from a cross between PA64s and 93-11. A total of 36 QTLs associated with shoot length, root length, shoot dry weight
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Marker-Assisted Breeding of Thermo-Sensitive Genic Male Sterile Line 1892S for Disease Resistance and Submergence Tolerance Rice Sci. (IF 3.162) Pub Date : 2020-12-16 Luo Yanchang; Ma Tingchen; Joanne Teo; Luo Zhixiang; Li Zefu; Yang Jianbo; Yin Zhongchao
Rice line 1892S is an elite thermo-sensitive genic male sterile (TGMS) line for two-line hybrid rice production. However, 1892S is susceptible to rice blast, bacterial blight and submergence. Here we reported the introduction of blast resistance (R) gene Pi9, bacterial blight R gene Xa21 and submergence tolerance gene Sub1A into 1892S genetic background through backcrossing and marker-assisted selection
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Development of Heat Tolerant Two-Line Hybrid Rice Restorer Line Carrying Dominant Locus of OsHTAS Rice Sci. (IF 3.162) Pub Date : 2020-12-16 Mehmood Jan; Gulmeena Shah; Huang Yuqing; Liu Xuejiao; Zheng Peng; Du Hao; Chen Hao; Tu Jumin
In order to create novel germplasm resources for breeding heat tolerant variety, we transferred a dominant allele OsHTAS, previously characterized and cloned from a high-temperature tolerant local variety HT54, which was collected from the rice production area of southern China, into a high- temperature sensitive intermediate breeding line HT13 through six rounds of successive backcross by using marker-assisted
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Progress on Molecular Mechanism of Aluminum Resistance in Rice Rice Sci. (IF 3.162) Pub Date : 2020-10-07 Chen Jingguang; Lai Qi; Zeng Baiquan; Guo Longbiao; Ye Guoyou
Aluminum (Al) toxicity in acid soils is a significant limitation to crop production worldwide, as 13% of the world's rice is produced in acid soil with high Al content. Rice is likely the most Al-resistant cereal and also the cereal, where Al resistance is the most genetically complex with external detoxification and internal tolerance. Many Al-resistance genes in rice have been cloned, including Al
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Development of Rice Leaves: How Histocytes Modulate Leaf Polarity Establishment Rice Sci. (IF 3.162) Pub Date : 2020-10-07 Wang Jiajia; Xu Jing; Qian Qian; Zhang Guangheng
An ideal leaf shape is beneficial to the yield of rice. Molecular understanding of the leaf primordia and polarity establishment plays a significant role in exploring the genetic regulatory network of leaf morphogenesis. In recent years, researchers have cloned an array of coding genes and a few non-coding small RNAs involved in rice leaf development through regulating the development of leaf primordia
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Paper New Allele of HL6 Regulates Trichome Elongation in Rice Rice Sci. (IF 3.162) Pub Date : 2020-10-07 Shang Fei; Mou Wenbin; Wu Hao; Xu Furong; Xiang Chunyan; Wang Jianfei
Trichomes are specialized epidermal cells that play multiple roles in plant development. However, knowledge about the molecular mechanism of trichome development in rice is limited. In this study, a hairy leaf locus HL6 SWWR of rice variety Suwangwanger (SWWR) was identified by map-based cloning. Transgenic complementation and knock-out mutation demonstrated that HL6SWWR regulated trichomes on leaves
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Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice Rice Sci. (IF 3.162) Pub Date : 2020-10-07 Li Ruili; Wang Jiaoling; Xu Lei; Sun Meihao; Yi Keke; Zhao Hongyu
The transport of phosphate between cytoplasm and subcellular compartments is critical for plant metabolic regulation. We conducted bioinformatic analysis, heterologous expression in yeast, gene expression pattern and subcellular localization analysis to characterize the possible functions of OsPHT4 gene family in rice. Together with the PHT4 genes from higher plants, OsPHT4s can be classified into
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Iksan526 Rice Callus Extract Induces Dedifferentiation of Rabbit Articular Chondrocytes via ERK1/2 and PI-3K/Akt Pathways Rice Sci. (IF 3.162) Pub Date : 2020-10-07 Seong-Hui Eo; Song Ja Kim
The resveratrol-enriched transgenic rice line Iksan526 (IS526), first developed by the Rural Development Administration of Korea using genetic engineering techniques, shows beneficial health effects in mitigating metabolic syndrome and obesity. However, the effects of IS526 on the differentiation of chondrocytes and the underlying mechanism have not been investigated in detail. In this study, the effects
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Effects of AG1 and AG2 QTLs on Nonstructural Carbohydrate and Seed Management Options for Rice Seedling Growth and Establishment under Flooding Stress Rice Sci. (IF 3.162) Pub Date : 2020-10-07 Satyen Mondal; M. Jamil Hasan; Tofayel Ahmed; M. Giashuddin Miah; Pompe C. Sta Cruz; Abdel M. Ismail
Rice (Oryza sativa) plants acquired excess photosynthates in the form of nonstructural carbohydrates (NSCs) in their stems and grain. Despite keen interest in rice NSC, the dynamics of NSC accumulation, translocation and re-accumulation have not yet been well investigated. AG1 and AG2 QTLs associated with flooding tolerance through catalyzing starch into soluble sugar in germinating seeds. Here we
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Putative Phosphatase UvPsr1 Is Required for Mycelial Growth, Conidiation, Stress Response and Pathogenicity in Ustilaginonidea virens Rice Sci. (IF 3.162) Pub Date : 2020-10-07 Xiong Meng; Meng Shuai; Qiu Jiehua; Shi Huanbin; Shen Xiangling; Kou Yanjun
Ustilaginoidea virens is the causal agent of rice false smut, which can be a highly destructive disease of rice. The plasma membrane phosphatase Psr1 proteins, which act as a regulator of the salinity stress response in yeast, are widely distributed across fungi, but their functional characterization is sketchy. In this study, we characterized the functions of Psr1 protein, UvPsr1, in U. virens. Analyses
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Salt Tolerance Mechanisms and Approaches: Future Scope of Halotolerant Genes and Rice Landraces Rice Sci. (IF 3.162) Pub Date : 2020-08-13 Tarun Bhatt, Aditi Sharma, Sanjeev Puri, Anu Priya Minhas
All rice plant developmental stages are severely affected by soil salinity. Salinity-induced ionic and osmotic stresses affect stomata closure and gaseous exchange, and reduce transpiration and the rate of carbon assimilation, and hence decrease plant yield. Understanding the response of rice plants toward salinity stress at the genetic level and developing salt-tolerant varieties are the vital mandates
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Roles of lncRNAs in Rice: Advances and Challenges Rice Sci. (IF 3.162) Pub Date : 2020-08-13 Gao Caixia, Zheng Xiuwen, Li Hubo, Ussi Ali Mlekwa, Gao Yu, Xiong Jie
Long non-coding RNAs (lncRNAs) larger than 200 nucleotides in length are eukaryotic RNAs, and do not encode proteins. lncRNAs are considered to be important regulators in many biological processes in plants. In this review, the latest advances in the roles of lncRNAs in rice were reviewed, and functions of lncRNAs were further summarized and classified. lncRNAs play crucial roles in tolerance to biotic
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A Fragment Substitution in Promoter of MS92/PTC1 Causes Male Sterility in Rice Rice Sci. (IF 3.162) Pub Date : 2020-08-13 Qin Peng, Deng Luchang, Chen Weilan, Huang Juan, Fan Shijun, Tu Bin, Tan Jun, Yuan Hua, Wang Yuping, Ma Bingtian, Li Shigui
Persistent tapetal cell1 (PTC1) plays a curial role in pollen development, and is thought to function as a transcriptional activator in rice. However, the molecular mechanism of PTC1 in regulating pollen development and its cis-elements are not well understood. We identified a novel weak male sterility mutant (ms92) which exhibited expanded tapetum and shrink pollen grains. Map-based cloning and allelic
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Effects of GS3 and GL3.1 for Grain Size Editing by CRISPR/Cas9 in Rice Rice Sci. (IF 3.162) Pub Date : 2020-08-13 Chen Yuyu, Zhu Aike, Xue Pao, Wen Xiaoxia, Cao Yongrun, Wang Beifang, Zhang Yue, Shah Liaqat, Cheng Shihua, Cao Liyong, Zhang Yingxin
Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a CRISPR/Cas9-mediated multiplex genome editing system was used to simultaneously edit GS3 and GL3.1 in a typical japonica rice Nipponbare. In T1 generation, we found that gs3 formed slender
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Identification of Rice QTLs for Important Agronomic Traits with Long-Kernel CSSL-Z741 and Three SSSLs Rice Sci. (IF 3.162) Pub Date : 2020-08-13 Wang Hui, Zhang Jiayu, Naz Farkhanda, Li Juan, Sun Shuangfei, He Guanghua, Zhang Ting, Ling Yinghua, Zhao Fangming
Rice kernel shape affects kernel quality (appearance) and yield (1000-kernel weight) and therefore is an important agronomic trait, but its inheritance is complicated. We identified a long-kernel rice chromosome segment substitution line (CSSL), Z741, derived from Nipponbare as a recipient and Xihui 18 as a donor parent. Z741 has six substitution segments distributed on rice chromosomes 3, 6, 7, 8
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Effects of Space Flight on Expression of Key Proteins in Rice Leaves Rice Sci. (IF 3.162) Pub Date : 2020-08-13 Zeng Deyong, Cui Jie, Yin Yishu, Zhang Meng, Shan Shan, Gao Xin, Zhang Yingchun, Lu Weihong, Sun Yeqing
As a unique form of abiotic stress, the environmental conditions of outer space are expected to induce changes in plant genomes, proteomes and metabolic pathways. However, the effect of outer space conditions on the overall physiology of plants at the protein level has yet to be reported. To investigate the effects of outer space conditions on the growth- and development-related physiological processes
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Mitigating N2O and NO Emissions from Direct-Seeded Rice with Nitrification Inhibitor and Urea Deep Placement Rice Sci. (IF 3.162) Pub Date : 2020-08-13 Yam Kanta Gaihre, Upendra Singh, Wendie D. Bible, Job Fugice, Joaquin Sanabria
Soil-emitted nitrous oxide (N2O) and nitric oxide (NO) in crop production are harmful nitrogen (N) emissions that may contribute both directly and indirectly to global warming. Application of nitrification inhibitors, such as dicyandiamide (DCD), and urea deep placement (UDP), are considered effective approaches to reduce these emissions. This study investigated the effects of DCD and UDP, compared
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Strategy for Use of Rice Blast Resistance Genes in Rice Molecular Breeding Rice Sci. (IF 3.162) Pub Date : 2020-06-13 Xiao Ning, Wu Yunyu, Li Aihong
Rice blast is one of the most destructive diseases affecting rice production worldwide. The development and rational use of resistant varieties has been the most effective and economical measure to control blast. In this review, we summarized the cloning and utilization of rice blast resistance genes, such as Pi1, Pi2, Pi9, Pi54, Pigm and Piz-t. We concluded that three main problems in the current
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Deciphering Rice Lesion Mimic Mutants to Understand Molecular Network Governing Plant Immunity and Growth Rice Sci. (IF 3.162) Pub Date : 2020-06-13 Zhu Xiaobo, Ze Mu, Chern Mawsheng, Chen Xuewei, Wang Jing
Plant lesion mimic mutants (lmms) generally possess autoimmunity and hypersensitive response (HR)-like cell death in the absence of biotic or abiotic stress. They have attracted much attention because they are useful tools for deciphering the interaction between defense signaling and growth. Recent studies have identified more than 30 lmms involved in the plant immune response and cell death in rice
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Alternative Splicing of OsRAD1 Defines C-Terminal Domain Essential for Protein Function in Meiosis Rice Sci. (IF 3.162) Pub Date : 2020-06-13 Yuan Shuting, Xu Chunjue, Yan Wei, Chang Zhenyi, Deng Xingwang, Chen Zhufeng, Wu Jianxin, Tang Xiaoyan
Alternative splicing can generate multiple mRNAs that differ in their untranslated regions or coding sequences, and these differences might affect mRNA stability or result in different protein isoforms with diverse functions and/or localizations. In this study, we isolated a sterile mutant in rice with abnormal meiosis of microspore mother cells and megaspore mother cells that carried a point mutation
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RNA-Seq Study Reveals AP2-Domain-Containing Signalling Regulators Involved in Initial Imbibition of Seed Germination in Rice Rice Sci. (IF 3.162) Pub Date : 2020-06-13 He Yongqi, Zhao Jia, Feng Defeng, Huang Zhibo, Liang Jiaming, Zheng Yufei, Cheng Jinping, Ying Jifeng, Wang Zhoufei
A number of internal signals are required for seed germination. However, the precise signalling responses in the initial imbibition of seed germination are not yet fully understood in rice. In this study, the RNA sequencing (RNA-Seq) approach was conducted in 8 h imbibed seeds to understand the signalling responses in the initial imbibition of rice seed germination. A total of 563 differentially expressed
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RNAi-Mediated Silencing of ITPK Gene Reduces Phytic Acid Content, Alters Transcripts of Phytic Acid Biosynthetic Genes, and Modulates Mineral Distribution in Rice Seeds Rice Sci. (IF 3.162) Pub Date : 2020-06-13 Aritra Karmakar, Sananda Bhattacharya, Shinjini Sengupta, Nusrat Ali, Sailendra Nath Sarkar, Karabi Datta, Swapan K. Datta
Phytic acid is the principal storage form of phosphorus in plant seeds and an essential signalling molecule in several regulatory processes of plant development. However, it is known as an anti-nutrient compound owing to its potent chelating property. Thus, reducing the phytic acid content in crops is desirable. Studies involving regulation of MIPS and IPK1 genes to generate low phytate rice have been
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Accumulation of Polyphenolic Compounds and Osmolytes under Dehydration Stress and Their Implication in Redox Regulation in Four Indigenous Aromatic Rice Cultivars Rice Sci. (IF 3.162) Pub Date : 2020-06-13 Nivedita Dey, Soumen Bhattacharjee
Present work was undertaken to screen some drought tolerant indigenous aromatic rice cultivars (IARCs), commonly cultivated in West Bengal, India, based on their capacity to produce osmolytes, redox-sensitive phenolic acids and flavonoids, as contrivances for redox-regulation under drought stress. Polyethylene glycol induced post imbibitional dehydration stress mediated changes in redox regulatory
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Responses of Lowland, Upland and Aerobic Rice Genotypes to Water Limitation During Different Phases Rice Sci. (IF 3.162) Pub Date : 2020-06-13 Preethi Vijayaraghavareddy, Yin Xinyou, Paul C. Struik, Udayakumar Makarla, Sheshshayee Sreeman
Rice yield reduction due to water limitation depends on its severity and duration and on the phenological stage of its occurrence. We exposed three contrasting rice genotypes, IR64, UPLRi7 and Apo (adapted to lowland, upland and aerobic conditions, respectively), to three water regimes (puddle, 100% and 60% field capacity) in pots during the vegetative (GSI), flowering (GSII) and grain filling (GSIII)
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Protein Phosphorylation and Phosphoproteome: An Overview of Rice Rice Sci. (IF 3.162) Pub Date : 2020-04-30 Abolore Adijat Ajadi, Amara Cisse, Shakeel Ahmad, Wang Yifeng, Shu Yazhou, Li Shufan, Liu Xixi, Babatunde Kazeem Bello, Sani Muhammad Tajo, Tong Xiaohong, Zhang Jian
Protein phosphorylation, one of the major post-translational modifications, plays a crucial role in cell signaling, DNA replication, gene expression and differentiation; and alters enzyme activity and other biological activities; and regulates cell proliferation and enlargement, phytohormone biosynthesis and signaling, plant disease resistance, and grain filling and quality during rice seed development
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Sugar Starvation Enhances Leaf Senescence and Genes Involved in Sugar Signaling Pathways Regulate Early Leaf Senescence in Mutant Rice Rice Sci. (IF 3.162) Pub Date : 2020-04-30 Li Zhaowei, Zhao Qian, Cheng Fangmin
To clarify the complex regulatory relationship between changes in sugar content and leaf senescence during the grain-filling stage of rice, genotype-dependent differences in sugar content and the temporal transcriptional patterns of genes involved in sugar signaling pathways were determined in mutant rice exhibiting early leaf senescence and its wild type Zhefu 142. The effects of exogenous glucose
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Verification of Glyphosate Resistance, Lepidopteran Resistance and Wide Compatibility of Male Sterile Line E1C4008S in Rice Rice Sci. (IF 3.162) Pub Date : 2020-04-30 Zeng Qiang, Deng Lihua, Hu Wenbin, He Xingjian, Meng Qiucheng, Yu Jianghui, Li Jinjiang, Weng Lushui, Xiao Guoying
Weeds and insect pests are two important biotic stresses resulting in yield loss in rice, and wide compatibility is the essential characteristic of breeding inter-subspecific hybrid rice. This study focused on glyphosate resistance, lepidopteran resistance and wide compatibility as well as identification of molecular and some agronomic characteristics of transgenic male sterile line E1C4008S. The results
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Genotypic Variation in Spatial Distribution of Fe in Rice Grains in Relation to Phytic Acid Content and Ferritin Gene Expression Rice Sci. (IF 3.162) Pub Date : 2020-04-30 Anurag Mishra, Md. Shamim, Md. Wasim Siddiqui, Akanksha Singh, Deepti Srivastava, K.N. Singh
Rice varieties having high Fe concentration in the endospermic region can be used as a good source for Fe deficit population. In this study, 303 Oryza sativa varieties and 1 Oryza rufipogon accession were assessed for spatial Fe accumulation in grains by Prussian blue staining method. Spatial ferritin protein distribution in grains was visualized by immunohistochemistry, and ferritin expression was
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Identification of Major Locus Bph35 Resistance to Brown Planthopper in Rice Rice Sci. (IF 3.162) Pub Date : 2020-04-30 Zhang Yuexiong, Qin Gang, Ma Qianqian, Wei Minyi, Yang Xinghai, Ma Zengfeng, Liang Haifu, Liu Chi, Li Zhenjing, Liu Fang, Huang Dahui, Li Rongbai
An introgression line RBPH660, derived from wild rice Oryza rufipogon, showed stable resistance to brown planthopper (BPH). Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs. By using the bulked segregant analysis (BSA)-seq method, two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to
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Association Mapping of Quantitative Trait Loci for Grain Size in Introgression Line Derived from Oryza Rufipogon Rice Sci. (IF 3.162) Pub Date : 2020-04-30 Kashif Hussain, Zhang Yingxing, Workie Anley, Aamir Riaz, Adil Abbas, Md. Hasanuzzaman Rani, Wang Hong, Shen Xihong, Cao Liyong, Cheng Shihua
Grain size is one of the critical agronomic traits governing grain yield and quality in rice. However, the underlying genetic mechanisms that control grain size in rice are poorly understood. We used an introgression line derived from Zhonghui 8015 and Oryza rufipogon Griff. This introgression line was evaluated under two different environmental conditions to dissect the quantitative trait loci controlling
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Rice Aroma: A Natural Gift Comes with Price and the Way Forward Rice Sci. (IF 3.162) Pub Date : 2020-02-27 Zakaria Hossain Prodhan, Shu Qingyao
Aromatic rice belongs to a small but important sub-group of rice, which is highly regarded for its excellent aroma and superior grain quality. Aromatic rice, especially Basmati- and Jasmine-type rice, is being traded at a high price in the local and global markets. Genetically, rice aroma is a phenotypical expression of spontaneous recessive mutations of the OsBadh2 gene (also known as fgr/badh2/osbadh2/os2AP
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Comprehensive Characteristics of MicroRNA Expression Profile Conferring to Rhizoctonia solani in Rice Rice Sci. (IF 3.162) Pub Date : 2020-02-27 Cao Wenlei, Cao Xinxin, Zhao Jianhua, Zhang Zhaoyang, Feng Zhiming, Ouyang Shouqiang, Zuo Shimin
MicroRNAs (miRNAs) are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses. However, it remains unknown whether miRNAs confer the resistance to necrotrophic fungus Rhizoctonia solani in rice. To investigate whether miRNAs regulate the resistance to R. solani, we constructed 12 small RNA libraries from susceptible
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Marker-Assisted Introgression of Quantitative Resistance Gene pi21 Confers Broad Spectrum Resistance to Rice Blast Rice Sci. (IF 3.162) Pub Date : 2020-02-27 Rosalyn B. Angeles-Shim, Vincent P. Reyes, Marilyn M. del Valle, Ruby S. Lapis, Junghyun Shim, Hidehiko Sunohara, Kshirod K. Jena, Motoyuki Ashikari, Kazuyuki Doi
The quantitative resistance gene pi21 from Sensho was introgressed to an indica breeding line IR63307-4B-13-2, a pyramiding line IRBB4/5/13/21, and a tropical japonica line Kinandang Patong by marker-assisted backcrossing. A total of 192 improved lines at the BC4F3 and BC4F4 generations were developed and confirmed to have the gene introgression via genotyping using a pi21-specific InDel marker. Thirteen
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Genetic Relationship and Structure Analysis of Root Growth Angle for Improvement of Drought Avoidance in Early and Mid-Early Maturing Rice Genotypes Rice Sci. (IF 3.162) Pub Date : 2020-02-27 Elssa Pandit, Rajendra Kumar Panda, Auromeera Sahoo, Dipti Ranjan Pani, Sharat Kumar Pradhan
Deeper rooting 1 (Dro1) and Deeper rooting 2 (Dro2) are the QTLs that contribute considerably to root growth angle assisting in deeper rooting of rice plant. In the present study, a set of 348 genotypes were shortlisted from rice germplasm based on root angle study. Screening results of the germplasm lines under drought stress identified 25 drought tolerant donor lines based on leaf rolling, leaf drying
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OsSRK1, an Atypical S-Receptor-Like Kinase Positively Regulates Leaf Width and Salt Tolerance in Rice Rice Sci. (IF 3.162) Pub Date : 2020-02-27 Zhou Jinjun, Ju Peina, Zhang Fang, Zheng Chongke, Bai Bo, Li Yaping, Wang Haifeng, Chen Fan, Xie Xianzhi
Receptor-like kinases (RLKs) are important for plant growth, development and defense responses. The S-receptor protein kinases (SRKs), which represent an RLK subfamily, control the self- incompatibility among Brassica species. However, little information is available regarding SRK functions in rice. We identified a gene OsSRK1 encoding an atypical SRK. The transcript of OsSRK1 was induced by abscisic
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Rice Heavy Metal P-type ATPase OsHMA6 Is Likely a Copper Efflux Protein Rice Sci. (IF 3.162) Pub Date : 2020-02-27 Zou Wenli, Li Chang, Zhu Yajun, Chen Jingguang, He Haohua, Ye Guoyou
P1B-type heavy metal ATPases (HMAs) are transmembrane metal-transporting proteins that play a key role in metal homeostasis. We here reported the characterization of rice OsHMA6, a member of the P1B-type ATPase family. Phylogenetic tree analysis showed that OsHMA6 belonged to the Cu/Ag subgroup of the HMA family and had a close evolutionary relationship with OsHMA9. Amino acid sequence alignment showed
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Expression Profiles and Protein Complexes of Starch Biosynthetic Enzymes from White-Core and Waxy Mutants Induced from High Amylose Indica Rice Rice Sci. (IF 3.162) Pub Date : 2020-02-27 Chen Yaling, Pang Yuehan, Bao Jinsong
Physicochemical properties of endosperm starches in milled rice determine its cooking and eating quality. Amylose is synthesized by granule-bound starch synthase I (GBSSI), whilst amylopectin is synthesized by the synergistic activities of starch synthases (SSs), branching enzymes (BEs) and debranching enzymes (DBEs). However, the complexes formed by starch biosynthetic enzymes are not well characterized
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Rhizosphere Aeration Improves Nitrogen Transformation in Soil, and Nitrogen Absorption and Accumulation in Rice Plants Rice Sci. (IF 3.162) Pub Date : 2020-02-27 Xu Chunmei, Chen Liping, Chen Song, Chu Guang, Wang Danying, Zhang Xiufu
Two rice cultivars (Xiushui 09 and Chunyou 84) were used to evaluate the effects of various soil oxygen (O2) conditions on soil nitrogen (N) transformation, absorption and accumulation in rice plants. The treatments were continuous flooding (CF), continuous flooding and aeration (CFA), and alternate wetting and drying (AWD). The results showed that the AWD and CFA treatments improved soil N transformation
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Differential Expression of Rice Valine-Qlutamine Gene Family in Response to Nitric Oxide and Regulatory Circuit of OsVQ7 and OsWRKY24 Rice Sci. (IF 3.162) Pub Date : 2019-12-13 Peng Xixu, Xiao Ting, Meng Jiao, Tao Zong, Zhou Dinggang, Tang Xinke, Wang Haihua
The functional diversity of plant valine-qlutamine (VQ) proteins is closely associated with their partners WRKY transcription factors, and also with a complex network of signaling pathways that mediated by hormone molecules. We reported genome-wide expression profiles of differentially expressed rice VQ genes under nitric oxide (NO) treatment based on a microarray analysis. Cluster analysis of expression
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Systematic Characterization of Long Non-Coding RNAs and Their Responses to Drought Stress in Dongxiang Wild Rice Rice Sci. (IF 3.162) Pub Date : 2019-12-13 Qi Weidong, Chen Hongping, Yang Zuozhen, Hu Biaolin, Luo Xiangdong, Ai Bing, Luo Yuan, Huang Yu, Xie Jiankun, Zhang Fantao
Long non-coding RNAs (lncRNAs) play important roles in response to various biotic and abiotic stresses. So far, systematic identification and characterization of lncRNAs have been reported in a few model plant species and major crops, but their roles in abiotic stress response have not yet been reported in common wild rice (Oryza rufipogon). Dongxiang wild rice (DXWR) possesses a high degree of drought
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Assessment of Variation in Morpho-Physiological Traits and Genetic Diversity in Relation to Submergence Tolerance of Five Indigenous Lowland Rice Landraces Rice Sci. (IF 3.162) Pub Date : 2019-12-13 Jijnasa Barik, Vajinder Kumar, Sangram K. Lenka, Debabrata Panda
The present study evaluated submergence responses in 88 lowland indigenous rice (Oryza sativa L.) landraces from Koraput, India, to identify submergence-tolerant rice genotypes. In pot experiments, variations in survival rate, shoot elongation, relative growth index, dry matter, chlorophyll, soluble sugar and starch contents were evaluated in two consecutive years under well-drained and completely
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Decrement of Sugar Consumption in Rice Young Panicle Under High Temperature Aggravates Spikelet Number Reduction Rice Sci. (IF 3.162) Pub Date : 2019-12-13 Wang Yaliang, Zhang Yikai, Shi Qinghua, Chen Huizhe, Xiang Jing, Hu Guohui, Chen Yanhua, Wang Xiaodan, Wang Junke, Yi Zihao, Zhu Defeng, Zhang Yuping
Two rice genotypes Huanghuazhan (HHZ, heat-resistant) and IR36 (heat-susceptible) were subjected to high-temperature (HT, 40 °C) and normal-temperature (NT, 32 °C) treatments at the spikelet differentiation stage. HT treatment inhibited spikelet differentiation, aggravated spikelet degeneration, reduced spikelet size, and disordered carbohydrate allocation. Meanwhile, HT treatment increased nonstructural
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Adoption and Impact of Modern Rice Varieties on Poverty in Eastern India Rice Sci. (IF 3.162) Pub Date : 2019-12-13 Richard Kwasi Bannor, Gupta Amarnath Krishna Kumar, Helena Oppong-Kyeremeh, Camillus Abawiera Wongnaa
The factors affecting the adoption of modern varieties (MVs) of rice and impact on poverty in Odisha, India were discussed. A total of 363 households from Cuttack and Sambalpur districts of Odisha via multistage sampling technique participated in the survey. The Cragg's Double hurdle model was used to model the determinants of adoption and intensity of adoption of MVs of rice, and the propensity score
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Morpho-Physiological Response of Oryza glaberrima to Gradual Soil Drying Rice Sci. (IF 3.162) Pub Date : 2019-12-13 Kartika Kartika, Jun-Ichi Sakagami, Benyamin Lakitan, Shin Yabuta, Andi Wijaya, Sabaruddin Kadir, Laily Ilman Widuri, Erna Siaga, Yoshihiro Nakao
Soil drought occurrence during dry season has been the main constraint, besides prolonged flooding during rainy season, in increasing cropping intensity and rice productivity in tropical riparian wetland. Use of drought tolerant rice genotype might be a suitable option for overcoming such problem. This study focused on the effects of gradual soil drying during early vegetative growth stage on morphological
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Optimization of High-Protein Glutinous Rice Flour Production Using Response Surface Method Rice Sci. (IF 3.162) Pub Date : 2019-12-13 Kanjanapa Eakkanaluksamee, Jirarat Anuntagool
A response surface method was employed to study the effect of α-amylase concentration, hydrolysis temperature and time on the production of high protein glutinous rice flour (HPGRF). The suspension of glutinous rice flour (15%) that contained 6.52% protein was gelatinized and subsequently hydrolyzed by thermostable α-amylase. The hydrolysis yielded 0.144–0.222 g/g HPGRF with 29.4%–45.4% protein content
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Sensing and Uptake of Nitrogen in Rice Plant: A Molecular View Rice Sci. (IF 3.162) Pub Date : 2019-10-11 Md. Saiful Islam
As a main feature of plant autotrophy, assimilation of inorganic nitrogen (N) is not only of fundamental interest to the crop, but also a crucial factor in crop productivity. N is the main plant mineral nutrient needed for chlorophyll production and other plant cell components (proteins, nucleic acids and amino acids). I highlighted the novel aspects of N responsive sensors, transporters and signaling
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Application of Micronutrients in Rice-Wheat Cropping System of South Asia Rice Sci. (IF 3.162) Pub Date : 2019-10-11 Faisal Nadeem, Muhammad Farooq
Rice-wheat cropping system (RWCS) is one of the most important cropping systems in South Asia. However, sustainability of this system is under threat owing to several factors, of which deficiency of micronutrients particularly zinc (Zn), boron (B) and manganese (Mn) is one of the major problems. Continuous rotation of rice and wheat, imbalanced fertilizer use and little/no use of micronutrient-enriched
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Characterization of a Novel Gain-of-Function Spotted-Leaf Mutant with Enhanced Disease Resistance in Rice Rice Sci. (IF 3.162) Pub Date : 2019-10-11 Chen Ting, Chen Zheng, Atul Prakash Sathe, Zhang Zhihong, Li Liangjian, Shang Huihui, Tang Shaoqing, Zhang Xiaobo, Wu Jianli
We here reported the identification and characterization of a novel gain-of-function spotted-leaf 26 (Spl26) mutant from an ethylmethylsulfone (EMS)-induced rice cultivar IR64. Spl26 displayed reddish- brown lesions that firstly appeared on the leaf tips at the early tillering stage and spread gradually downward to cover the whole leaf blades that wilted subsequently. The lesion development was light-
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Genome-Wide Association Analysis and Allelic Mining of Grain Shape-Related Traits in Rice Rice Sci. (IF 3.162) Pub Date : 2019-10-11 Lv Yang, Wang Yueying, Noushin Jahan, Hu Haitao, Chen Ping, Shang Lianguang, Lin Haiyan, Dong Guojun, Hu Jiang, Gao Zhenyu, Qian Qian, Zhang Yu, Guo Longbiao
Excavating single nucleotide polymorphisms (SNPs) significantly associated with rice grain shape and predicting candidate genes through genome-wide association study (GWAS) can provide a theoretical basis for discovery and utilization of excellent genetic resources in rice. Based on 16 352 SNPs, 161 natural indica rice varieties with various grain sizes in southern China were used for GWAS of grain
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Genetic Diversity and Allelic Frequency of Selected Thai and Exotic Rice Germplasm Using SSR Markers Rice Sci. (IF 3.162) Pub Date : 2019-10-11 Wanwarang Pathaichindachote, Natjaree Panyawut, Kannika Sikaewtung, Sujin Patarapuwadol, Amorntip Muangprom
A collection of 167 Thai and exotic rice accessions was subjected for evaluation of genetic diversity and assessment of relationship by simple sequence repeat (SSR) markers. Among a total of 49 SSR markers, 13 markers distributing over 12 rice chromosomes showed clear polymorphic band patterns, and they were selected for genetic assessment. A total of 110 alleles were detected with an average of 8
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Soil Nitrogen Distribution and Plant Nitrogen Utilization in Direct-Seeded Rice in Response to Deep Placement of Basal Fertilizer-Nitrogen Rice Sci. (IF 3.162) Pub Date : 2019-10-11 Wang Danying, Ye Chang, Xu Chunmei, Wang Zaiman, Chen Song, Chu Guang, Zhang Xiufu
Deep placement of controlled-release fertilizer increases nitrogen (N) use efficiency in rice planting but is expensive. Few studies on direct-seeded rice have examined the effects of deep placement of conventional fertilizer. With prilled urea serving as N fertilizer, a two-year field experiment with two N rates (120 and 195 kg/hm2) and four basal N application treatments (B50, all fertilizer was
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Impact of Rice-Catfish/Shrimp Co-culture on Nutrients Fluxes Across Sediment-Water Interface in Intensive Aquaculture Ponds Rice Sci. (IF 3.162) Pub Date : 2019-10-11 Liu Yaobin, Qin Lin, Li Fengbo, Zhou Xiyue, Xu Chunchun, Ji Long, Chen Zhongdu, Feng Jinfei, Fang Fuping
Exchange of nitrogen and phosphorus across sediment-water interface plays an important role in the management of nutrient recycling in the aquaculture pond. In this study, a plot experiment was conducted to study the effect of rice-catfish/shrimp co-culture on the micro-profile of oxygen (O2), pH and nutrient exchange across sediment-water interface in the intensive culture ponds. The results showed
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CRISPR/Cas9: Development and Application in Rice Breeding Rice Sci. (IF 3.162) Pub Date : 2019-09-09 Fernando Matías Romero, Andrés Gatica-Arias
Rice (Oryza sativa L.) is an important staple food crop worldwide due to its adaptability to different environmental conditions. Because of its great economic and social importance, there is a constant requirement for new varieties with improved agronomic characteristics, such as tolerance to different biotic (such as bacterium, fungus, insect and virus) and abiotic stresses (such as salinity, drought
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Differential RNA Editing of Mitochondrial Genes in WA-Cytoplasmic Based Male Sterile Line Pusa 6A, and Its Maintainer and Restorer Lines Rice Sci. (IF 3.162) Pub Date : 2019-09-09 Umakanta Ngangkham, Swarup Kumar Parida, Ashok Kumar Singh, Trilochan Mohapatra
RNA editing changes the nucleotides at the transcript level of mitochondrial genes which results in synthesis of functional proteins. This study was designed to find the editing sites which could be implicated in male fertility restoration and to develop editing based markers for differentiation of cytoplasmic male sterility and maintainer lines from each other. DNA and RNA from young panicles were
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Fusarium solani Upregulated Sesquiterpene Synthase Expression, Sesquiterpene Production and Allelopathic Activity in Piper betle L. Rice Sci. (IF 3.162) Pub Date : 2019-09-09 Kamrai Woranoot, Rattikarn Buaruang, Kodchakorn Aranyakanon, Kumrop Ratanasut, Anupan Kongbangkerd, Panatda Jannoey, Pranee Nangngam, Chonnanit Choopayak
Activators of sesquiterpene synthase (STS) gene expression and sesquiterpene production in Piper betle L. were examined using quantitative real time PCR and gas chromatography mass spectrometry methods, and the allelopathic activity of untreated and Fusarium solani-treated betel extracts was tested on seed germination and on the shoot and root growth of Thai rice variety PSL2 (Oryza sativa cv. Phitsanulok
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