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  • 更新日期:2020-01-15
  • Thermal protection and pH-gated release of folic acid in microparticles and nanoparticles for food fortification
    Food Funct. (IF 3.241) Pub Date : 2020-01-15
    Ilja Gasan Osojnik Crnivec; Katja Istenič; Mihaela Skrt; N.P. Ulrih

    Encapsulation provides efficient approaches to increase stability and delivery of poorly soluble bioactive components, predominantly for fortification of beverages and similar liquid-based foods. In this study, folic acid was encapsulated within conventional and emulsion-templated alginate–pectin hydrogels, proliposomes, and a combination thereof. The stability of these systems was examined under various environmental conditions (pH 1.2-9.0, 25-85 °C, dark/light). The techniques demonstrated efficient and relatively straightforward production of well-defined microparticles and nanoparticles (350 nm to 250 µm). Dispersed folic acid provided a delivery system with unique pH-responsive features, which offered prolonged stability during food storage, and indicated increased release at the site of absorption upon ingestion. This formulation had no limitation due to particle size, while at the same time it allowed high encapsulation efficiencies (80%-100%), as compared to the low encapsulation efficiency achieved by solubilisation (6%). Under simulated gastric conditions, leaching of the dispersed folic acid was prevented, while at the expected intestinal pH, there was complete release via solubilisation and carrier swelling. Overall, the optimum for food processing and storage was pH 3.0, where ≥70% of 50% to 200% recommended daily allowance of folic acid remained in the alginate–pectin beads after 6 months at room temperature in the dark. The thermal properties were enhanced by emulsion-templated alginate–pectin beads and proliposomes. In this way, 30% to 75% retention of folic acid was achieved at temperatures ≤90 °C, where the proliposomes reinforced within a polysaccharide network achieved the highest level of protection.

    更新日期:2020-01-15
  • Synthesis and in-vitro fecal fermentation of potential prebiotic -glucooligosaccharides using microbial glucansucrase
    Food Funct. (IF 3.241) Pub Date : 2020-01-15
    Xiuting Hu; Liping Song; Yuqi Yang; Zhengyu Jin; Ming Miao

    Novel -glucooligosaccharides were synthesized by the acceptor reaction of Leuconostoc citreum SK24.002 glucansucrase with maltose and sucrose. The impact of synthesis conditions, including the ratio of sucrose to maltose and the substrate concentration, on formation of -glucooligosaccharide was evaluated. At the optimized experimental conditions, the yield of a mixture of -glucooligosaccharides with DP 3-5 reached approximately 56.4% with the concentration of 170.7 mg/mL. Each of these -glucooligosaccharides was purified, and the structures were assigned as follows: α-D-Glcp-(1,6)-α-D-Glcp-(1,4)-D-Glcp (DP3), α-D-Glcp-(1,3)-α-D-Glcp-(1,6)-α-D-Glcp-(1,4)-D-Glcp (DP4), and α-D-Glcp-(1,6)-α-D-Glcp-(1,3)-α-D-Glcp-(1,6)-α-D-Glcp-(1,4)-D-Glcp (DP5), respectively. Of three structurally different oligosaccharides, the fermentation selectivity by fecal bacteria was determined in anaerobic batch culture. Fructooligosaccharide (FOS) was used as a positive prebiotic control. Similar to FOS, all three -glucooligosaccharides selectively stimulated the proliferation of Bifidobacteria and Lactobacilli compared with the control. DP3 exerted the strongest prebiotic ability for increasing the Bifidobacteria and Lactobacilli population, whereas DP5 produced the most short-chain fatty acids. In addition, DP4 produced the highest butyrate concentration and resulted in the lowest acetate: propionate ratio. These results suggested that the enzymatically-synthesized -glucooligosaccharides were potential prebiotics, underlining correlations between structural features of oligosaccharides and their impact on the metabolism of fecal microbiota.

    更新日期:2020-01-15
  • Reduction of serum cholesterol and its mechanism by Lactobacillus plantarum H6 screened from local fermented food products
    Food Funct. (IF 3.241) Pub Date : 2020-01-14
    Tianming Qu; Liu Yang; Yuhua Wang; Bin Jiang; Minghao Shen; Dayong Ren

    Hypercholesterolemia is the leading cause of cardiovascular disease worldwide. In this study, the lactic acid bacteria (Lactobacillus plantarum H6) screened from homemade fermented foods in northeastern China were used to study their cholesterol-lowering ability and to analyze their degradation mechanism. Results showed that L. plantarum H6 significantly reduced serum cholesterol levels in C57BL/6 mice fed a hypercholesterolemia diet. It mainly promoted the expression of CYP7A1 gene by inhibiting the farnesoid X receptor pathway to increase the in vivo synthesis of bile acids and improved the intestinal microbial community structure of C57BL/6 mice to increase the abundance of bacterial flora containing bile salt hydrolase activity. Hence, L. plantarum H6 played a role in reducing the cholesterol content in mice.

    更新日期:2020-01-14
  • Colloidal characteristics, emulsifying activities, and interfacial properties of α-lactalbumin-chitosan electrostatic complexes: effects of mass ratio and pH
    Food Funct. (IF 3.241) Pub Date : 2020-01-14
    Yuexiang Liu; Yuting Fan; Xuli Wu; Yujuan Lu; Yi Jiang

    The essential hydrophobic carrier properties of α-lactalbumin (α-LA): chitosan (CHI, Mw 100 kDa, degree of deacetylation 75-85%) including mass ratio and pH on the characteristics, emulsifying activities, and interfacial properties of α-LA-CHI complexes were investigated. The interaction between α-LA and CHI was dominated by electrostatic interactions. Soluble α-LA-CHI complex can be formed at both pH 6.0, and 6.5 at mass ratios of 1:1, 2:1, and 5:1, as shown by the optical appearance. UV-vis spectra demonstrated that the interaction between α-LA and CHI is highly CHI concentration, and pH-dependent. AFM graphs showed that α-LA-CHI complexes are spherically shaped and homogeneously dispersed in solution, with diameters around 100-250 nm, in accordance with DLS results, though some aggregates were observed for the 1:1 α-LA-CHI complex. The emulsifying activity of α-LA was enhanced by complexation with CHI. Both emulsifying activity index (EAI) and emulsifying stability index (ESI) of α-LA-CHI complexes were associated with mass ratio and pH values. Interfacial tension of α-LA-CHI complex was negatively linearly correlated with EAI. The lower the interfacial tension of α-LA-CHI complex on oil-water interface, the higher the EAI. The results obtained from this study were complementary and provided insights into the interaction between α-LA and CHI, and suggested that α-LA-CHI complexes can be utilized as effective food ingredients for stabilizing, protecting, and delivering hydrophobic nutraceuticals.

    更新日期:2020-01-14
  • α-Lactalbumin and chitosan core−shell nanoparticle: resveratrol loading, protection, and antioxidant activity
    Food Funct. (IF 3.241) Pub Date : 2020-01-14
    Yuexiang Liu; Luyu Gao; Yi Jiang; Yuting Fan; Xuli Wu; Yuzhu Zhang

    Resveratrol (RES)-loaded protein-polysaccharide nanoparticles were fabricated by simple electrostatic interactions with oppositely charged α-Lactalbumin (ALA) and chitosan (CHI) with a mass ratio of 5:1 without NaCl addition at pH 6.5. Z-average diameter and zeta-potential values of RES-ALA-CHI nanoparticle were 211.0 nm, and 13.23 mV, respectively. Both TEM and AFM graphs confirmed RES-ALA-CHI nanoparticle were nanoscale, in spherical shape, and dispersed homogeneously. Encapsulation efficiency (EE) and loading amount (LA) of RES in RES-ALA-CHI nanoparticle were 58.86%, and 196.2 μg/mg in the presence of 400 μg/mL RES, respectively. RES was in amorphous form, testified with XRD results. The interaction between RES and ALA-CHI nanoparticle was mainly driven by hydrophobic interaction and hydrogen bonding. Compared to RES (free), the UV light, and heat stability, in vitro bioaccessibility, and antioxidant activity of RES in RES-ALA-CHI nanoparticle were pronouncedly enhanced. The information provided in this study should be of interest to food industry to fabricate robust nanoscale delivery systems with ALA-CHI nanoparticle for RES and other hydrophobic bioactive compounds.

    更新日期:2020-01-14
  • Hydrogels assembled from ovotransferrin fibrils and xanthan gum as dihydromyricetin delivery vehicles
    Food Funct. (IF 3.241) Pub Date : 2020-01-14
    Zihao Wei; Yongsheng Chen; Wahyu Wijaya; Yujia Cheng; Jie Xiao; Qingrong Huang

    The present study aimed to assemble protein fibril–polysaccharide hydrogels as nutraceutical delivery vehicles. Turbidity titrations confirmed that complexations between ovotransferrin (OVT) fibril and xanthan gum (XG) indeed existed, and electrostatic interaction was the major driving force of OVT fibril–XG complexation. After optimization of pH and acidifer, stable OVT fibril–XG hydrogel could be fabricated by adjusting pH to 4.0 with glucono delta-lactone. To better understand physicochemical properties of OVT fibril–XG gel, characterization of XG gel was also conducted. Scanning electron microscopy indicated that OVT fibril–XG gel had denser network than XG gel. Rheological measurements revealed that OVT fibril–XG gel had higher gel strength and viscosity than XG gel. OVT fibril–XG gel and XG gel could be applied as dihydromyricetin (DMY) delivery vehicles with a higher DMY loading (2 mg/mL). DMY release was investigated using in vitro gastrointestinal digestion model. All of DMY was released from OVT fibril–XG gel after gastrointestinal digestion, and only 41.7% of DMY was released from XG gel after gastrointestinal digestion, indicating that OVT fibril–XG gel was more efficient in DMY delivery. DMY was released via non-Fickian transport mechanism in both OVT fibril–XG gel and XG gel. The results in this study could provide new insight into assembly of protein fibril–polysaccharide hydrogels and rational design of hydrogels as nutraceutical delivery vehicles.

    更新日期:2020-01-14
  • EPA-Enriched Ethanolamine Plasmalogen and EPA-Enriched Phosphatidylethanolamine Enhance BDNF/TrkB/CREB Signaling and inhibit Neuronal Apoptosis in Vitro and in Vivo
    Food Funct. (IF 3.241) Pub Date : 2020-01-14
    Hongxia Che; Lingyu Zhang; Lin Ding; Wancui Xie; Xiaoming Jiang; Changhu Xue; Tiantian Zhang; Yuming Wang

    Our previous study showed that EPA-enriched ethanolamine plasmalogen (EPA-pPE) exerted more significant effects than EPA-enriched phosphatidylethanolamine (EPA-PE) in improving the learning and memory deficit. However, the results of mechanism study were not consistent with the improved cognitive function, which suggested other signaling pathways might be involved. In the present study, primary cultured hippocampal neurons and cognitive deficiency rats were used to compare the effects of EPA-pPE and EPA-PE on brain-derived neurotrophic factor (BDNF)/tropomyosin receptor kinase B (TrkB)/cAMP response element-binding protein (CREB) signaling and neuronal apoptosis. The in vitro experiment showed that both EPA-pPE and EPA-PE could relieve the cell death and improve the cellular morphology of neurons via upregulating anti-apoptotic proteins and downregulating pro-apoptotic proteins. The in vivo experiment showed that EPA-pPE exerted more significant effects than EPA-PE in improving the number of neuronal nissl bodies, increasing the branching of dendrites and dendritic spine density in cortical neurons, as well as improving the expression of synaptic vesicle-related proteins synaptophysin (SYN) and PSD95 via BDNF/TrkB/CREB signaling. These results indicated that EPA-pPE exerted neuroprotection at least partly through inhibiting neuronal apoptosis and enhancing the BDNF/TrkB/CREB pathway, which suggests that EPA-enriched plasmalogen can be explored as a potential therapeutic agent in long-term Alzheimer’s disease therapy.

    更新日期:2020-01-14
  • Protective effect of Lachnum polysaccharide on dextran sulfate sodium-induced colitis in mice†
    Food Funct. (IF 3.241) Pub Date : 2020-01-10
    Shuai Zong; Ziyang Ye; Xinmiao Zhang; Hui Chen; Ming Ye
    更新日期:2020-01-14
  • Reliable, accessible and transferable method for the quantification of flavanols and procyanidins in foodstuffs and dietary supplements†
    Food Funct. (IF 3.241) Pub Date : 2020-01-08
    Ugo Bussy; Brian R. May; Yusuf Olanrewaju; Gregory Hewitt; Nicholas Anderson; Alan Crozier; Javier I. Ottaviani; Catherine Kwik-Uribe
    更新日期:2020-01-14
  • In vitro digestibility and bioaccessibility of lipid-based delivery systems obtained via enzymatic glycerolysis: a case study of rosemary extract bioaccessibility
    Food Funct. (IF 3.241) Pub Date : 2020-01-06
    Marta Corzo-Martínez; Celia Bañares; Alejandro Díaz; Luis Vázquez; Guillermo Reglero; Carlos F. Torres
    更新日期:2020-01-14
  • Citrus flavonoids suppress IL-5 and ROS through distinct pathways in PMA/ionomycin-induced EL-4 cells
    Food Funct. (IF 3.241) Pub Date : 2019-12-27
    Wei-Ling Yang; Sheng-Yi Chen; Cheng-Ying Ho; Gow-Chin Yen
    更新日期:2020-01-14
  • Purified plant cell walls with adsorbed polyphenols alter porcine faecal bacterial communities during in vitro fermentation†
    Food Funct. (IF 3.241) Pub Date : 2019-12-19
    Lucas J. Grant; Deirdre Mikkelsen; Anh Dao T. Phan; Seungha Kang; Diane Ouwerkerk; Athol V. Klieve; Michael J. Gidley; Barbara A. Williams
    更新日期:2020-01-14
  • Pregnancy complications-dependent change in SIgA-targeted microbiota during the third trimester
    Food Funct. (IF 3.241) Pub Date : 2020-01-13
    Mengjun Cui; Ce Qi; Liping Yang ; Mengyun Zhang; Huiyan Wang; Guangtong She; renqiang yu; Tingting Miao; Jin Sun

    Gut microbiota plays a crucial role in metabolic dysfunction during gestation, which might be prevented using probiotics. This study compared the composition of gut microbiota in healthy and complicated pregnancy, for screening and isolating healthy pregnancy-derived probiotics. According to the principal component analysis of secretory immunoglobulin A (SIgA)-coated microbiota in gut, third trimester volunteers can be divided into three groups: AHd (n = 29), GDMd (n = 37), and GHd (n = 25), dominated by asymptomatic healthy donors (62.07%), gestational diabetes mellitus (GDM) donors (40.54%), and gestational hypertension (GH) donors (40%), respectively. There was a significant difference in β-diversity (p<0.01) and α-diversity (p<0.05) among the three groups. At the phylum level, Firmicutes of the GHd group were significantly lower than those of the AHd group (p = 0.039), while Bacteroidetes (p = 0.005) and Proteobacteria (p = 0.002) of the GHd group were more dominant than those of AHd group. At the genus level, linear discriminant analysis effect size showed that SIgA-targeted Enterococcus was the dominant taxonomic biomarker of AHd group, and the GHd group was enriched with Escherichia and Streptococcus. The GDMd and GHd groups had higher faecal calprotectin, serum lipopolysaccharide, zonulin, and GLYCAM-1 levels. We conclude that the occurrence of complications in the third trimester may be related to intestinal barrier injury associated with disorders of intestinal SIgA-targeted microbiota, gut barrier injury triggers inflammation in pregnant women. SIgA-targeted L. reuteri showed significant correlation with low inflammatory response and may be a potential probiotic candidate for preventing pregnancy complications.

    更新日期:2020-01-13
  • Tangeretin mitigates L-NAME-induced ventricular dysfunction and remodeling through AT1R/pERK1/2/pJNK signaling pathway in rats
    Food Funct. (IF 3.241) Pub Date : 2020-01-13
    Chutamas Wunpathe; Putcharawipa Maneesai; Siwayu Rattanakanokchai; Sarawoot Bunbupha; Upa Kukongviriyapan; Terdthai Tong-un; Poungrat Pakdeechote

    Tangeretin is a citrus flavonoid that exerts several beneficial effects, including anti-inflammation, anti-oxidation and neuroprotection. For this study, the aim was to test the effect of tangeretin on Nω-Nitro-L-arginine methyl ester (L-NAME)-induced high blood pressure, left ventricular dysfunction and remodeling in rats. Rats were divided into five groups (n=8/each group): a control group, an L-NAME group and three L-NAME groups treated with tangeretin (15mg/kg) or tangeretin (30 mg/kg) or captopril (5 mg/kg) for the final two weeks. After five weeks of experiment, L-NAME groups had high systolic blood pressures, ventricular dysfunction and remodeling. Overexpression of angiotensin II type 1 receptor, phosphorylated-extracellular-regulated kinase 1/2 (pERK1/2), phosphorylated-c-Jun N-terminal kinases (pJNK) protein but downregulation of endothelial nitric oxide synthase (eNOS) protein expression in ventricular tissues were shown in hypertensive rats while protein expression of phosphorylated-mitogen activated protein kinase p38 did not differ among groups. Decrease in plasma NOx and increases in vascular superoxide generation, plasma malondialdehyde, angiotensin-converting enzyme activity and angiotensin II levels were found in hypertensive rats. These alterations were suppressed in hypertensive rats treated with tangeretin or captopril. In conclusion, tangeretin exhibits antihypertensive effects and alleviates ventricular dysfunction and remodeling in hypertensive rats. These effects are associated with inhibition of renin angiotensin system activation and restoration of pERK1/2, pJNK, eNOS protein expressions along with reducing oxidative stress and raising NO bioavailability

    更新日期:2020-01-13
  • 更新日期:2020-01-13
  • The preventive effect of phenolic-rich extracts from Chinese sumac fruits against nonalcoholic fatty liver disease in rats induced by a high-fat diet
    Food Funct. (IF 3.241) Pub Date : 2019-12-30
    Zihuan Wu; Yan Zhang; Xiarong Gong; Guiguang Cheng; Shibiao Pu; Shengbao Cai
    更新日期:2020-01-13
  • Mild heat combined with lactic acid fermentation: a novel approach for enhancing sulforaphane yield in broccoli puree
    Food Funct. (IF 3.241) Pub Date : 2019-12-26
    Yan Xue Cai; Mary Ann Augustin; Hema Jegasothy; Ji Hui Wang; Netsanet Shiferaw Terefe
    更新日期:2020-01-10
  • EFFECTS OF FRUCTOSE-RICH DIET AND CHRONIC STRESS ON INSULIN SIGNALING AND REGULATION OF GLYCOGEN SYNTHASE KINASE-3 BETA AND SODIUM-POTASSIUM PUMP IN THE HEART OF MALE RATS
    Food Funct. (IF 3.241) Pub Date : 2020-01-09
    Snjezana Romic; Ana Djordjevic; Snezana Tepavcevic; Tijana Culafic; Mojca Stojiljkovic; Biljana Bursac; Jelena Stanisic; Milan Kostic; Ljupka Gligorovska; Goran Koricanac

    Both diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine effects of fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether those effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress during 9 weeks. Protein expression and/or phosphorylation of insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by Western blot. GR distribution between cytosolic and nuclear fraction was also analyzed. Fructose-rich diet decreased level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased IRS1 content and prevented fructose diet-induced decrease of pAkt (Ser473) level. Fructose-rich diet in combination with chronic stress reduced protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased protein content of Na+/K+-ATPase α1- and α2-subunit, while after combined treatment α2 content was at the control level and α1 content was lower than control. Effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights on effects of chronic stress and combination of fructose diet and chronic stress on studied molecules in the heart.

    更新日期:2020-01-09
  • Screening of Lactobacillus salivarius strains from the feces of Chinese populations and the evaluation of their effects against intestinal inflammation in mice†
    Food Funct. (IF 3.241) Pub Date : 2020-01-04
    Qixiao Zhai; Xudan Shen; Shi Cen; Chengcheng Zhang; Fengwei Tian; Jianxin Zhao; Hao Zhang; Yuzheng Xue; Wei Chen
    更新日期:2020-01-09
  • Prediagnostic dietary intakes of vitamin A and β-carotene are associated with hepatocellular-carcinoma survival†
    Food Funct. (IF 3.241) Pub Date : 2019-12-26
    Dao-ming Zhang; Yun Luo; Dinuerguli Yishake; Zhao-yan Liu; Tong-tong He; Yan Luo; Yao-jun Zhang; Ai-ping Fang; Hui-lian Zhu
    更新日期:2020-01-09
  • Strawberry (Fragaria × ananassa cv. Romina) methanolic extract promotes browning in 3T3-L1 cells
    Food Funct. (IF 3.241) Pub Date : 2019-12-26
    Tamara Y. Forbes-Hernández; Danila Cianciosi; Johura Ansary; Bruno Mezzetti; Stefano Bompadre; Josè L. Quiles; Francesca Giampieri; Maurizio Battino
    更新日期:2020-01-09
  • 更新日期:2020-01-09
  • Evolution of important glucosinolates in three common Brassica vegetables during their processing into vegetable powder and in vitro gastric digestion
    Food Funct. (IF 3.241) Pub Date : 2019-12-06
    Thitima Kuljarachanan; Nan Fu; Naphaporn Chiewchan; Sakamon Devahastin; Xiao Dong Chen
    更新日期:2020-01-09
  • Dose-response effect on polyphenols bioavailability after intake of white and red wine pomace products in Wistar rats.
    Food Funct. (IF 3.241) Pub Date : 2020-01-08
    Gisela Gerardi; Monica Cavia-Saiz; María Dolores Rivero-Perez; María Luisa González-SanJosé; Pilar Muñiz

    Wine pomace by-products are an important source of phenolic acids with significant health benefits. However, phenolic acid bioavailability in vivo is not well studied and there are few comparative studies on bioavailability between red and white wine pomaces and the effect of different doses of intake. Qualitative and quantitative profiling of phenolic acid metabolites and antioxidant capacity in plasma and urine samples from Wistar rats is performed following oral administration of four doses (50, 100, 150, and 300 mg) of red and white wine pomace products (rWPP and wWPP, respectively).The phenolic metabolites, ABTS and FRAP levels of the plasma and urine samples were evaluated. The results showed that neither the bioavailability nor the antioxidant capacity in vivo of the rWPP increased at high doses. However, both parameters were dependent on the intake of the wWPP.

    更新日期:2020-01-08
  • Benefits of the ipowder® extraction process applied to Melissa officinalis L.: improvement of antioxidant activity and in vitro gastro-intestinal release profile of rosmarinic acid
    Food Funct. (IF 3.241) Pub Date : 2020-01-08
    Valérie Bardot; Anaïs Escalon; Isabelle Ripoche; Sylvain Denis; Monique Alric; Sandrine Chalancon; Pierre Chalard; César Cotte; Lucile Berthomier; Martin Leremboure; Michel Dubourdeaux
    更新日期:2020-01-08
  • Integrated phytochemical analysis based on UHPLC-LTQ-Orbitrap and network pharmacology approach to explore the potential mechanism of Lycium ruthenicum Murr. for ameliorating Alzheimer's disease
    Food Funct. (IF 3.241) Pub Date : 2020-01-08
    Zhiqiang Luo; Guohua Yu; Xinjing Chen; Yang Liu; Yating Zhou; Guopeng Wang; Yuanyuan Shi

    Based on compelling experimental and clinical evidence, the fruit of Lycium ruthenicum Murr. (LRM), a unique traditional Tibetan medicine, exerts beneficial effects on ameliorating learning and memory deficits of Alzheimer's disease (AD) and other neurodegenerative disorders. However, the potential active constituents and biological mechanism of LRM are still unknown. In this study, the major chemical constituents of LRM were first analyzed by ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap). A total of 35 constituents were confirmed or tentatively identified. Furthermore, the network-based pharmacological strategy was applied to clarify the molecular mechanism of LRM on AD based on the identified components. Totally, 143 major targets were screened and supposed to be effective players in alleviating AD. Then, the LRM chemicals-major LRM putative targets-major pathways network was constructed, implying potential biological function of LRM on AD. More importantly, 12 core genes which can be modulated by LRM were identified, and they may play a pivotal role in alleviating some major symptoms of AD. This study provided a scientific basis for further investigation and application of LRM, which demonstrated that the network pharmacology approach could be a powerful way for the mechanistic studies of folk medicines.

    更新日期:2020-01-08
  • Effects of Irradiation on Structure and Properties of Glycosylated Soybean Protein
    Food Funct. (IF 3.241) Pub Date : 2020-01-08
    Yuying Wang; Anqi Zhang; Yixiao Wang; Xibo Wang; Ning Xu; Lianzhou Jiang

    At present, there are few reports on the glycosylation modification of soybean protein under irradiation. In this paper, soybean protein isolated and maltose were used as raw materials to prepare the glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure. The results of analysis of graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that maillard reaction between soybean protein isolate and maltose occurred and the structure of the reaction product changed. When the irradiation dose was 7.5kGy, the solubility of modified products increased by 23±0.21% compared with the control group. Foaming property and foam stability increased by 62.5±0.34% and 41±0.47%, respectively. Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly. Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement. This experiment combined irradiation technology with glycosylation modification method. It was proved that the irradiation could promote the maillard reaction between soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of soy protein isolate in food industry.

    更新日期:2020-01-08
  • Analysis of the anti-fatigue activity of polysaccharide from Spirulina platensis: Role of central 5-hydroxytryptamine mechanisms.
    Food Funct. (IF 3.241) Pub Date : 2020-01-08
    ju mei zhu; zhu zhu; ming ding; sheng liu; hua zou

    This study is to evaluate the effects of polysaccharide from Spirulina platensis (PSP) on treadmill exercise endurance, the levels of some biochemical indicators as hemoglobin (Hb), Lactic acid (LA), creatine kinase (CK) and urea nitrogen (BUN) in the blood, 5-hydroxytryptamine (5-HT) concentrations, the second isoforms of tryptophan hydroxylase (TPH2) and serotonergic type 1B inhibitory autoreceptors (5-HT1B) expression in the caudate putamen of exercised rats. Sixty Sprague-Dawley male rats were randomly divided into six groups: control group, exercise group, exercise and PSP (50, 100,200 mg/kg)–treated groups, exercise and caffeine (10 mg/ kg)–treated group (positive control). In exercise groups, rats were put on treadmill and forced to run for 30 min once a day for 6 consecutive days. On the 7th day of the experiment, the time to exhaustion in treadmill exercise was determined for the trained groups. Immediately after the determination of the exhaustion time, all rats were sacrificed. The levels of Hb and LA were tested by the HiCN (Hemoglobin ferricyanide) colorimetry method and colorimetric assay method, respectively. The levels of CK and BUN were determined by automatic biochemical analyser. 5-HT concentrations were detected by HPLC analysis. TPH2 and 5-HT1B expression were measured by western blot analysis and real-time PCR. The results demonstrated that PSP could prolong the time to exhaustion in treadmill exercise, increase Hb levels and decrease LA, BUN and CK levels in the blood and suppress the exercise-induced increase of 5-HT concentrations and TPH2 expression and prevent the exercise-induced decrease of 5-HT1B expression in the caudate putamen. The most potent effects were observed at the dose of 200 mg/kg of PSP. It suggests that the mechanism of PSP’s promoting physical performance might be related to increasing Hb levels and decreasing LA, BUN and CK levels in the blood and the inhibition of the exercise-induced synthesis of 5-HT and TPH2 expression, and the increase of the 5-HT1B expression in the caudate putamen of exercised rats.

    更新日期:2020-01-08
  • The effect of bound polyphenols on the fermentation and antioxidant properties of carrot dietary fiber in vivo and in vitro
    Food Funct. (IF 3.241) Pub Date : 2019-12-23
    Shuai Liu; Qiang Yu; Hairong Huang; Kunyou Hou; Ruihong Dong; Yi Chen; Jianhua Xie; Shaoping Nie; Mingyong Xie
    更新日期:2020-01-08
  • Luteolin reduces fat storage in Caenorhabditis elegans by promoting the central serotonin pathway†
    Food Funct. (IF 3.241) Pub Date : 2019-12-23
    Yan Lin; Nan Yang; Bin Bao; Lu Wang; Juan Chen; Jian Liu
    更新日期:2020-01-08
  • The effect of regular consumption of lupin-containing foods on glycaemic control and blood pressure in people with type 2 diabetes mellitus†‡
    Food Funct. (IF 3.241) Pub Date : 2019-12-16
    Natalie C. Ward; Trevor A. Mori; Lawrence J. Beilin; Stuart Johnson; Carolyn Williams; Seng Khee Gan; Ian B. Puddey; Richard Woodman; Michael Phillips; Emma Connolly; Jonathan M. Hodgson
    更新日期:2020-01-08
  • Effects of three cooking methods on content changes and absorption efficiencies of carotenoids in maize
    Food Funct. (IF 3.241) Pub Date : 2020-01-07
    Songhao Zhang; Jing Ji; Siqi Zhang; Chunfeng Guan; Gang Wang

    Maize is a staple source of certain carotenoids for the human diet, but food processing is an important factor affecting the carotenoid content and absorption. In this study, we investigated the content changes of carotenoids in maize under the three cooking methods (boiling kernels, porridge and tortilla). Also, using the in vitro digestion model, we assessed the effects of cooking methods on carotenoid absorption efficiencies (digestion stability, micellization efficiency and bioaccessibility). The results indicated that the carotenoid content obviously increased in the boiling kernels, but the carotenoid bioaccessibility was the lowest comparing to porridge and tortilla. Tortilla presented the highest digestion stability of β-carotene (309 ± 63 %) and the bioaccessibility of xanthophylls (22.4 ± 0.5 % for lutein and 18.5 ± 1.0 % for zeaxanthin) among three cooking products during the in vitro digestion. The contents of carotenoids in porridge were all the lowest in three cooking products, however, the low concentration level of xanthophylls reduced the competitive effect on the β-carotene micellization, which increased the bioaccessibility of β-carotene to 52.1 ± 5.0 %. Additionally, the content of xanthophylls (lutein + zeaxanthin) in digesta significantly and positively correlated with the β-carotene content in digesta, whereas negatively correlated with the micellization of β-carotene. And this correlation between the xanthophylls and β-carotene was not affected by the cooking methods. These results together suggest that tortilla and porridge are the better dietary choices to intake xanthophylls and β-carotene, respectively, among maize-based foods. Furthermore, the absorption of β-carotene was influenced by the content of xanthophylls whatever the cooking method.

    更新日期:2020-01-07
  • Bilberry anthocyanin improves the neuroinflammation and cognitive disfunctions in APP/PSEN1 mice via the CD33/TREM2/TYROBP signaling pathway in microglia
    Food Funct. (IF 3.241) Pub Date : 2020-01-07
    Jing Li; Runtian Zhao; Yuhan Jiang; Yi Xu; Huan Zhao; Xiaoling Lyu; Tao Wu

    Alzheimer’s disease, characterized by neuroinflammation and beta-amyloid protein plaques, is a memory-threatening neurodegenerative disease with no effective treatments. Here, the effect of bilberry anthocyanin (BA) on cognitive functions was evaluated using APP/PSEN1 transgenic Alzheimer’s disease model mice and their WT littermates. Our results revealed that BA appreciably improves learning and memory abilities and reverses defects to cognitive functions in APP/PSEN1 mice. Furthermore, BA reverses brain, liver and kidney damages caused by Alzheimer's disease, with no significant changes in oxidative stress and lipid metabolism-related indicators. In addition, BA decreases serum and brain lipopolysaccharides (LPS) levels and increases fecal short-chain fatty acids content. Immunofluorescence and RT-PCR analyses results showed that BA fully activates the microglia and astrocytes, downregulates the expression of inflammatory factors (TNF-a, NF-Kβ, IL-1β, IL-6, COX-2, iNOS and CD33) and chemokine receptor CX3CR1, and upregulates the expression of microglia homeostatic factors (TREM2 and TYROBP) and Toll-likem (TLR2 and TLR4). Moreover, Western blot analysis revealed that BA significantly upregulates the expression of synaptic and phagocytotic function-related proteins (CD68 and IRF7) in APP/PSEN1 mice. Altogether, we show for the first time that BA consumption reverses Alzheimer’s disease‑induced cognitive disfunction, decreases hippocampal neuroinflammatory responses, and induces phagocytosis of microglia to beta-amyloid protein plaques by regulating the CD33/TREM2/TYROBP signaling pathway in microglia.

    更新日期:2020-01-07
  • Human-origin Lactobacillus salivarius AR809 protects against immunosuppression in S. aureus-induced pharyngitis via Akt mediated NF-κB and autophagy signaling pathways
    Food Funct. (IF 3.241) Pub Date : 2020-01-07
    Guochao Jia; Xiaofeng Liu; Na Che; YongJun Xia; Guangqiang Wang; Zhiqiang Xiong; Hui Zhang; Lianzhong Ai

    Lactobacillus salivarius AR809 from healthy human pharynx, is a newly discovered probiotic strain possessing potential ability to adhere to the pharyngeal epithelium and inhibit Staphylococcus aureus (S. aureus)-induced inflammatory response. Pharyngeal spray administration of AR809 exhibited protective effects in a S. aureus-induced mouse model of pharyngitis. The inhibitory effect and underlying molecular mechanism of AR809 on S. aureus-stimulated pharyngitis were further investigated. AR809 significantly increased phagocytosis and bactericidal activity, reduced the production of inflammatory mediators (intracellular reactive oxygen species (ROS), prostaglandin E2 (PGE2), Cyclooxygenase-2 (COX-2), nitric oxide (NO), inducible NOS (iNOS)) and the expression of inflammatory cytokine (Tumor necrosis factor-a (TNF-a), Interleukin-1β (IL-1β)), and induced macrophages to adopt the M2 phenotype. AR809 also attenuated S. aureus-induced phosphorylations of protein kinase B (Akt), rapamycin (mTOR), and elevated the autophagic protein (light chain 3 from II (LC3-II), Beclin-1) level. Furthermore, AR809 inhibited nuclear transcription factor kappa-B (NF-κB) activation by suppressing the nuclear translocation of the NF-κB p65. Likewise, 740Y-P (a PI3K activator) decreased the anti-inflammatory effect of AR809 against S. aureus-induced inflammatory response, while AR809 treatments with wortmannin (a PI3K inhibitor) markedly reversed this inflammatory response. AR809 prevents S. aureus-induced pharyngeal inflammatory response, possibly by regulation of TLR/PI3K/Akt/mTOR signalling pathway-related autophagy and TLR/PI3K/Akt/IκB/NF-κB pathway activity, and therefore has potential for use in preventing pharyngitis and other inflammatory diseases.

    更新日期:2020-01-07
  • Enhancement of the anti-inflammatory properties of grape pomace treated by Trametes versicolor
    Food Funct. (IF 3.241) Pub Date : 2019-12-19
    Ana Bucić-Kojić; Fátima Fernandes; Tânia Silva; Mirela Planinić; Marina Tišma; Gordana Šelo; Darijo Šibalić; David M. Pereira; Paula B. Andrade
    更新日期:2020-01-07
  • Dietary fiber isolated from sweet potato residues promotes a healthy gut microbiome profile†
    Food Funct. (IF 3.241) Pub Date : 2019-12-19
    Man Liu; Xuezhi Li; Shengmin Zhou; Thomas T. Y. Wang; Sihan Zhou; Kunlong Yang; Yongxin Li; Jun Tian; Jing Wang
    更新日期:2020-01-07
  • Baicalein reduces hepatic fat accumulation by activating AMPK in oleic acid-induced HepG2 cells and high-fat diet-induced non-insulin-resistant mice
    Food Funct. (IF 3.241) Pub Date : 2019-12-19
    Wenlong Sun; Panpan Liu; Tianqi Wang; Xudong Wang; Weilong Zheng; Jingda Li
    更新日期:2020-01-07
  • Vanillin enhances the passive transport rate and absorption of drugs with moderate oral bioavailability in vitro and in vivo by affecting the membrane structure†
    Food Funct. (IF 3.241) Pub Date : 2019-12-17
    Wen'ge Huang; Yingzhuo Yang; Wen Wen; Yu Luo; Jiasi Wu; Li Xiang; Yinfan Hu; Shijun Xu; Simin Chen; Ping Wang
    更新日期:2020-01-07
  • Microbiota modulation and effects on metabolic biomarkers by orange juice: a controlled clinical trial
    Food Funct. (IF 3.241) Pub Date : 2020-01-06
    Melaine Priscila Fidélix; Dragan Milenkovic; Thais Cesar; Katia Sivieri

    The impact of habitual orange juice consumption on microbiota, lipid and glycides metabolism was investigated in a controlled clinical trial. Ten women who had a regular diet without orange juice for 30 days (OJ-free diet), followed by a regular diet plus 300 ml/d orange juice for 60 days (OJ-Diet), and 30 days with regular diet without orange juice (Washout). It was monitoring biochemical and dietary parameters, and blood, urine and stool samples were collected every 30 days until the end of the study. By UHPLC, it was identified hesperidin and naringin metabolites in the urine, and the microbiota composition by r16S RNA in the feces. At the end of OJ-Diet, there was a reduction in glucose (-6.5%), insulin (-33%), insulin resistance (-44%), LDL-C (-16%) and triglycerides (-30%). After the washout, these parameters returned to their initial values. There were no changes in body weight or fat during the experiment time. The intestinal bacteria, Lactobacillus spp., Arkkermansia spp., and Ruminococcus spp., increased after the intervention with orange juice. In addition, it was detected an inverse correlation between these bacterias and glycemia, insulin, HOMA-IR, triglycerides, total cholesterol and LDL-C, but direct correlation with HDL-C. In conclusion, orange juice showed a prebiotic effect, modulating the intestinal microbiota while improving glycemia and lipid profile.

    更新日期:2020-01-06
  • Anti-obesity effects of α-amylase inhibitor enriched-extract from white common beans (Phaseolus vulgaris L.) associated with the modulation of gut microbiota composition in high-fat diet-induced obese rats
    Food Funct. (IF 3.241) Pub Date : 2020-01-06
    Zhenxing Shi; Ying-ying Zhu; Cong Teng; Yang Yao; Guixing Ren; Aurore Richel

    α-Amylase inhibitor (α-AI) has great potential to treat obesity. In this study, α-Amylase inhibitorAI enriched extract (α-AIE) with specific activity of 1027.1±154.2 (U mg-1 protein) were prepared from white common bean (Phaseolus vulgaris L.) seeds. has great potential to treat obesity. Base on its starch blocker role, the indirect modulating effect on the gut microbiota composition was hypothesized. This study aimed to verify Itsthe anti-obesity effect and the gut microbiota modulation properties were verified of α-AIE in high-fat diet-induced obese rats. Intake of α-AIE significantly reduced the body weight gain and improved the serum lipid levels of obese rats (p<0.05). In addition, rats fed the α-AIE diet exhibited higher total short-chain fatty-acid (SCFA) concentrations (p<0.05) in their colonic contents. β-Diversity analysis, principal components analysis and a Venn diagram showed that α-AIE administration changed the gut microbiota composition of obese rats. At the phylum level, the relative abundances of Firmicutes and Proteobacteria decreased and the relative abundances of Bacteroidetes and Akkermansia increased. In addition, 89 operational taxonomic units (OTUs) significantly responding to high-fat diet and 30 OTUs significantly responding to α-AIE were identified. The OTUs enriched by α-AIE were mainly assigned to putative SCFA-producing bacteria, including Bacteriodes, Butyricoccus, Blautia and Eubacterium. Twenty-two OTUs were found to be significantly correlated with obesity indexes. Taken together, the present results suggest that intake of α-AIE attenuated obesity and modulated gut microbiota.

    更新日期:2020-01-06
  • Effect of regular and decaffeinated roasted coffee (Coffea arabica and Coffea canephora) extracts and bioactive compounds on in vitro probiotic bacteria growth
    Food Funct. (IF 3.241) Pub Date : 2020-01-06
    Amanda Sales; Juliana DePaula; Caroline Mellinger ; Adriano Gomes da Cruz; Marco Antonio Miguel; Adriana Farah

    The aim of this study was to investigate the effect of coffee species, roast degree and decaffeination on in vitro probiotic bacteria growth, and to identify major coffee compounds responsible for such effect. Six C. arabica and C. canephora extracts (regular medium and dark roasted and decafeinated medium roasted), and five bioactive compounds (chlorogenic acid, galactomannan, type 2 arabinogalactan, caffeine and trigonelline) were individually incorporated into a modified low-carbon broth medium-(mMRS), at different concentrations (0.5 to 1.5% soluble coffee and 0.05 to 0.8mg/mL standard solutions). Inulin and fructooligossacharides (FOS) were used as prebiotic references. MRS and mMRS were used as rich and poor media controls, respectively. The growth of Lactobacillus rhamnosus GG ATCC 53103-(GG), L. acidophilus LA-5-(LA), Bifidobacterium animalis DN-173010-(BA) and B. animalis subsp. lactis BB12-(BB12), as well as the growth inhibition of non-probiotic Escherichia coli ATCC 25922 were evaluated. Differences in growth between mMRS and treatments (∆ Log CFU/mL) were compared by ANOVA and Tukey test, and considered when p ≤ 0.05. Overall, after 48h incubation, medium roasted arabica coffee extract increased the growth of GG, LA and BA (range ∆ Log CFU/mL = 0.5 to 1.8), while dark roasted arabica coffee extract increased BB12 growth (range ∆ Log CFU/mL = 0.9 to 1.7), in a dose dependent manner. Improved performances of GG, LA and BA were promoted by higher polysacharides and CGA concentrations, with better performance for Lactobacillus sp. The tested coffee bioactive compounds promoted poor growth of BB12. Plain caffeine did not promote Bifidobacterium sp. growth and limited the growth of Lactobacillus sp. Regular C. arabica and C. canephora extracts inhibited the growth of E.coli, while the decaffeinated extracts promoted its growth. The present results show that coffee consumption can selectively improve the growth of probiotic strains, thus exerting a prebiotic effect, and show that coffee roasting and decaffeination affect this property and that different strains utilize different coffee components to grow.

    更新日期:2020-01-06
  • Sesamol: A powerful functional food ingredient from sesame oil for cardioprotection
    Food Funct. (IF 3.241) Pub Date : 2020-01-06
    PREMKUMAR JAYARAJ; CHANDRAKALA ALUGANTI NARASIMHULU ; SANJAY RAJAGOPALAN; S. Parthasarathy; Desikan Rajagopal

    Phytophenols are important bioactive food based chemical entities, largely present in several natural sources. Among them, sesamol is one of the key natural phenols found in sesame seeds and piper cubeba etc. Several studies have reported that sesame oil is a potent cardioprotective functional food. Papers on the utility of sesamol in sesame oil (Chemical name of sesamol is methylenedioxyphenol-MDP) have appeared in the literature, though, there is no single concise review on the usefulness of seamol in sesame oil in CVD is available in the literature. Cardiovascular disease (CVD) is the most challenging health problems encountered by global population. There has been an increasing interest in the growth of effective cardiovascular therapeutics, specifically of natural origin. Among various natural sources of chemicals, phytochemicals are micronutrients and bio-compatible scaffolds having extraordinary efficacy at the multiple disease targets with minimal or no adverse effect. This review offers a perspective on the existing literature on functional food in sesame oil with particular focus on sesamol and its derivatives having nutritional and cardioprotective properties. This is demonstrated to have shown specifically modulating oxidative enzyme, myeloperoxidase (MPO), and other proteins which are detrimental to human well-being. The molecular mechanism of cardioprotection by this food ingredient, primarily attributed to methylenedioxy group present in sesamol component.

    更新日期:2020-01-06
  • Ameliorative Effects of Resveratrol against Cadmium-induced Nephrotoxicity via Modulating Nuclear Xenobiotic Receptors Response and PINK1/Parkin-mediated Mitophagy
    Food Funct. (IF 3.241) Pub Date : 2020-01-03
    Qi Zhang; Cong Zhang; Jing Ge; Mei-Wei Lv; Milton Talukder; Kai Guo; Li Yanhua; Yan Zhu; Jin-Long Li

    Cadmium (Cd) is a toxic pollutant with high nephrotoxicity in agriculture environment. Resveratrol has been expounded a renoprotective effect but the underlying mechanisms of this have not been fully elucidated. The aim of the current study was to illustrate the antagonism of resveratrol against Cd-induced nephrotoxicity. Total 80 birds were divided randomly into 4 groups and treated as follows: control group (Con), 400mg/kg resveratrol group (Resv), 140mg/kg Cd group (Cd 140) and 140 mg/kg Cd+400 mg/kg resveratrol group (Cd+Resv) in diet for 90 days. It was observed that resveratrol treatment dramatically alleviated Cd-induced histopathological lesions of kidney. Simultaneously, resveratrol mitigated Cd-induced oxidative stress by reducing MDA and H2O2 production, alleviating GSH depletion and restoring antioxidant enzymes (T-SOD, Cu-Zn SOD, CAT, GST and GSH-Px) activity. Resveratrol activated NXRs (CAR/PXR/AHR/Nrf2) signaling pathways and exerted antidotal roles by enhanced phase I and II detoxification system to relief oxidative damage. Moreover, resveratrol ameliorated Cd-induced ultrastructural abnormal and mitochondria dysfunction by recovering mitochondrial function related factors VDAC1, Cyt C and Sirt3 upregulation and Sirt1, PGC-1α, Nrf1 and TFAM transcription restrictions. Resveratrol attenuated Cd-induced excessive mitochondrial fission and promoted mitochondria fusion, which rescued PINK1/Parkin-mediated mitophagy initiation. Collectively, our findings explicated a potential protection against Cd-induced nephrotoxicity and mitochondria damage.

    更新日期:2020-01-04
  • Trehalose and alginate oligosaccharides increase the stability of muscle proteins in frozen shrimp (Litopenaeus vannamei)
    Food Funct. (IF 3.241) Pub Date : 2020-01-03
    Bin Zhang; Hui Yao; He Qi; Xiao-li Zhang

    Muscle proteins in whiteleg shrimp (Litopenaeus vannamei) are susceptible to denaturation and are affected by freezing and frozen storage. Cryoprotective trehalose (TR) and alginate oligosaccharides (AO) can slow the degradation of myofibrillar proteins and reduce mechanical damage on shrimp muscle tissues. We conducted label-free based proteomic analysis to study the stability of proteins in TR- and AO-treated shrimp at -18°C after 30 d of frozen storage. A total of 2155 peptides and 626 proteins were identified and quantified in shrimp muscle. The most common 95 differentially abundant proteins (DAPs) were down-regulated and detected in distilled water (DW), TR, and AO, especially the down-regulated DAPs including the actin, calcium-transporting ATPase, myosin heavy and light chains, paramyosin, tropomyosin, and troponin. Most of the above DAPs were up-regulated in TR/AO compared to DW. This was most likely due to the incorporation of TR/AO molecules into the muscle tissues, resulting in decreased protein degradation and reduced damage from ice crystal growth. Gene Ontology (GO) analysis indicated that these DAPs were mainly involved in binding, catalytic activity, cell, cell parts, cellular process, and metabolic process GO items, which were associated with protein structure, function, and metabolism. Kyoto Encyclopedia of Genes and Genomes (KEGG) results suggested alteration of many pathways, including metabolic pathways, carbon metabolism, phototransduction, phagosome, carbon metabolism, and ribosome, that are involved with arginine kinase, L-lactate dehydrogenase, malate dehydrogenase, phosphoglycerate mutase, and ribosomal proteins. The TR/AO treatment maintained the natural conformation, structure, and function of the DAPs and delayed degradation of muscle proteins. Here, this study provided a deeper insight into the protein changes in shrimp muscle during frozen storage and could be a valuable foundation for future investigations.

    更新日期:2020-01-04
  • Correction: Protective effects of hydroalcoholic extracts from an ancient apple variety ‘Mela Rosa dei Monti Sibillini’ against renal ischemia/reperfusion injury in rats
    Food Funct. (IF 3.241) Pub Date : 2020-01-02
    Hasan Yousefi-Manesh; Sara Hemmati; Samira Shirooie; Seyed Mohammad Nabavi; Azita Talebzadeh Bonakdar; Reza Fayaznia; Mohammad Hossein Asgardoon; Ali Zare Dehnavi; Mohammad Ghafouri; Joice G. Nkuimi Wandjou; Giovanni Caprioli; Stefania Sut; Filippo Maggi; Stefano Dall'Acqua

    Correction for ‘Protective effects of hydroalcoholic extracts from an ancient apple variety ‘Mela Rosa dei Monti Sibillini’ against renal ischemia/reperfusion injury in rats’ by Hasan Yousefi-Manesh et al., Food Funct., 2019, 10, 7544–7552.

    更新日期:2020-01-02
  • A Pilot Study on the Effects of DHA/EPA Enriched Phospholipids on Aerobic Exercise and Anaerobic Exercise in Mice
    Food Funct. (IF 3.241) Pub Date : 2020-01-02
    Chengcheng Wang; Lin Ding; Lingyu Zhang; Hao-hao Shi; Changhu Xue; Naiqiu Chi; Teruyoshi Yanagita; Tiantian Zhang; Yuming Wang

    The beneficial effects of nonpolar DHA/EPA in triacylglycerol (TG) and ethyl ester (EE) forms as well as terrestrial phospholipids on physical fatigue have been widely reported. However, the results involving the effects were inconsistent, and the reason might be that it usually ignored the differences between physical fatigue induced by aerobic and anaerobic exercise. Additionally, it has been reported the significant improvement of DHA/EPA esterified to phospholipids (DHA/EPA-PLs) on many fields but not physical fatigue. Therefore, the effects of DHA/EPA-PLs on physical fatigue induced by aerobic and anaerobic exercise were evaluated and compared with L-carnitine and astaxanthin using swimming and running exhaustion test in mice, respectively. The results showed that DHA/EPA-PL and L-carnitine had significant effects on the performance of aerobic exercise, while astaxanthin had remarkable effect on the performance of anaerobic exercise. The possible underlying mechanisms indicated that DHA/EPA-PL significantly promoted the carbohydrate and lipid metabolism as well as mitochondrial respiratory chain and tricarboxylic acid cycle in muscle. The study represented a potential novel candidate or targeted dietary patterns for alleviating physical fatigue.

    更新日期:2020-01-02
  • Drying of African Leafy Vegetables for Their Effective Preservation: Difference in Moisture Sorption Isotherms Explained by their Microstructure
    Food Funct. (IF 3.241) Pub Date : 2020-01-02
    Leonie van 't Hag; Jessica Danthe; Stephan Handschin; Gibson P Mutuli; Duncan Mbuge; Raffaele Mezzenga

    The problem of malnutrition and nutrition deficiency, as well as droughts that lead to reduction in food supply and starvation, are well documented for Sub-Saharan Africa. Reducing post-harvest losses of five species of African leafy vegetables (ALVs) by preservation through drying is studied herein. Energy efficient gentle drying conditions using superabsorbent polymers and a temperature of 40 oC was shown to preserve most leave structures and vitamins. The microbial safe moisture content of the ALVs was found to be ≤ 14% dry basis. Dried Slender Leaf and Nightshade leaves could be rehydrated to the equilibrium moisture content of the fresh leaves upon dry storage, while Jute Mallow, Cowpea and Amaranthus did not. This were attributed to different palisade parenchyma cell lengths. An increased amount of starch granules as observed in the microstructure of Cowpea and Nightshade leaves are suggested to explain their fibrous texture upon cooking. These results show that the ALVs can effectively be preserved using the same drying method and that this can be used to fight micro-nutrient deficiencies during droughts

    更新日期:2020-01-02
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  • Structural analysis and allergenicity assessment of an enzymatically cross-linked bovine α-lactalbumin polymer
    Food Funct. (IF 3.241) Pub Date : 2019-12-09
    Xin Li; Hao Bai; Yuanyuan Wu; Wei Cheng; Yong Wu; Zhihua Wu; Anshu Yang; Ping Tong; Hongbing Chen
    更新日期:2020-01-02
  • 9t18:1 and 11t18:1 activate the MAPK pathway to regulate the expression of PLA2 and cause inflammation in HUVECs
    Food Funct. (IF 3.241) Pub Date : 2019-12-02
    Sheng-Ben Hu; Qian Zou; Xin Lv; Ruo-Lin Zhou; Xian Niu; Chen Weng; Fang Chen; Ya-Wei Fan; Ze-Yuan Deng; Jing Li
    更新日期:2020-01-02
  • Characterising absorption and health-related properties of phytochemicals extracted from Malaysian palm fruit biomass after oil extraction
    Food Funct. (IF 3.241) Pub Date : 2019-12-31
    Sophie Selby-Pham; Lee Fong Siow; Louise Bennett

    After extraction of oil, palm fruit biomass contains a rich source of water-soluble phytochemicals (PCs) with proven bioactivity in regulating oxidative stress and inflammation (OSI). For optimal bioefficacy, the pharmacokinetic plasma peak (Tmax) should be bio-matched with the onset of OSI, which can be predicted from the Phytochemical Absorption Prediction (PCAP) model and methodology. Predicted absorption and potential for regulation of OSI by measures of total phenolic content, antioxidant capacity and hydrogen peroxide production capacity, were applied to characterise eight extracts from mesocarp fibre and kernel shells of oil-depleted palm fruits. Results indicated post-consumption absorption Tmax ranges of 0.5–12 h and 2–6 h for intake in liquid and solid forms, respectively, and generally high antioxidant activity, of palm fruit PCs. The research supports that PC extracts of palm fruit biomass have broad potential uses for human health as dietary antioxidants in foods, supplements or functional beverages.

    更新日期:2019-12-31
  • Effect of high energy electron beam on proteolysis and antioxidant activity of rice proteins
    Food Funct. (IF 3.241) Pub Date : 2019-12-31
    Xinxia Zhang; Li Wang; Zheng-Xing Chen; Yongfu Li; Xiaohu Luo; Yanan Li

    This research focused on the effects of electron beam irradiation (EBI) on the hydrolysis and antioxidant activity of rice proteins (RPs). The RPs were treated with 0, 5, 10, 20 and 30 kGy doses of EBI. The results showed that EBI pretreatment improved significantly (P < 0.05) the degree of hydrolysis, increasing the DH value by more than 15.09% at a dose of 30 kGy. In addition, radical scavenging results showed that EBI treatment had effects on antioxidant activity and could increase the DPPH and ABTS+ radical scavenging activity of rice protein hydrolysates (RPHs) by 32.06% and 79.11%, respectively (30 kGy). The CAA test also confirmed that EBI pretreatment could effectively improve the ability of RPHs to remove intracellular free radicals. Scanning electron microscopy indicated that EBI treatment destroyed microstructures and resulted in cracks and fragments of RPs. Circular dichroism analysis showed that EBI affected the secondary structure of RPs by destroying the α-helix structure. Changes in the UV visible spectra indicated unfolding of RPs by EBI. Amino acid and molecular weight distribution analysis revealed that EBI pretreatment could increase the ratio of antioxidant-related amino acids and produce smaller peptides. Therefore, EBI pretreatment is an efficient method to promote protein proteolysis due to its effect on the molecular conformation as well as on the microstructure of the protein. Moreover, EBI treatment applied before enzymatic hydrolysis has the potential to prepare hydrolysates with high bioactivity.

    更新日期:2019-12-31
  • Acrylamide in French fries prepared at primary school canteens
    Food Funct. (IF 3.241) Pub Date : 2019-12-30
    Marta Mesías; Cristina Delgado-Andrade; Francisca Holgado; Francisco J. Morales

    Children are one the most exposed groups to dietary acrylamide, ‘Potato fried products’ accounting for up to half the total exposure to this contaminant. Acrylamide was determined in French fries prepared in 31 primary school canteens randomly recruited from different Spanish regions. The mean content was 329 μg kg-1 (from < 20 to 4000 μg kg-1). French fries prepared from frozen par-fried potato reported lower acrylamide content than those from fresh potato, 229 and 460 µg kg-1, respectively. Only 15.7% of samples were above the benchmark levels established by the EU Regulation 2017/2158 (500 µg kg-1). Significant differences were found according to the color for toasted (2274 µg kg-1), dark-golden (463 µg kg-1), golden (134 µg kg-1) and light-golden (52 µg kg-1) French fries. All the samples sorted as golden and light-golden showed acrylamide content below the threshold. Chromatic parameter a* was able for a rough classification of the French fries according to the benchmark level. Both educational initiatives intended to food operators and managerial-technical criteria to include in the code of frying practices at the public food service establishments should consider the golden color as the target for the end-point of frying. The acrylamide exposure will be reduced and, therefore, the risk linked to the French fries’ consumption. This fact turns out especially relevant in establishments preparing foods for schoolchildren and would help to provide healthier diets, not only from a nutritional point of view but also from the reduction of chemical contaminants.

    更新日期:2019-12-30
  • The Mechanism of Improved Thermal Stability of Protein-enriched O/W Emulsions by Soy Protein Particles with Anti-aggregation Property
    Food Funct. (IF 3.241) Pub Date : 2019-12-30
    Chao Wu; Wuchao Ma; Jiamei Wang; Di Wu; Hui Chen; Ming Du

    Growing interest in nutritional and functional foods has motivated the design of protein-enriched products in food industry, which, however, is greatly challenged by undesirable aggregation and gelation of proteins induced by heating from pasteurization process. In this study, we reported the preparation of heat-stable soy protein particles (SPPs) by a simple preheat process (100 °C for 30 min) at pH 6.2 and 0.5% (w/v) protein concentration. As a proof-of-concept, the thermal stability of high-protein emulsions prepared by SPPs compared to native soy proteins (SPs) were investigated. Results showed that high-protein emulsions stabilized by SPPs exhibited appreciable heat stability whereas SPs gelled when both samples were tested at an identical concentration (10%, w/v). In addition, the emulsions prepared by SPPs demonstrated lower values of storage modulus and viscosity in relationship with stabilized size by heat treatment as well as more stable coated protein layer, in contrast to those prepared by SPs presenting macroscopic aggregation and instable coated protein layer. The results would provide valuable information in terms of the development of heat-stable, high-protein, and well-dispersed food emulsions that may find numerous applications in food industry.

    更新日期:2019-12-30
  • Anti-Parkinson's disease activity of phenolic acids from Eucommia ulmoides Oliver leaf extracts and its autophagy activation mechanism
    Food Funct. (IF 3.241) Pub Date : 2019-12-30
    Shanshan Zhang; Zhangping Yu; Jingyu Xia; Xuanming Zhang; Kechun Liu; Attila Sik; Meng Jin

    Although Parkinson’s disease (PD) is the second most common neurodegenerative disorder, the preventative or therapeutic agents for the treatment of PD are limited. Eucommia ulmoides Oliver (EuO) is widely used as traditional herb to treat various diseases. EuO bark extracts have been reported to possess anti-PD activity. Here, we investigated whether extracts of EuO leaves (EEuOL) also have therapeutic effect on PD since similar components and clinical application have been found between barks and leaves of this tree. We identified the chemical composition of EEuOL by HPLC-Q-TOF-MS and tested the anti-PD effect of EEuOL using zebrafish PD model. As a result, 28 compounds including 3 phenolic acids, 7 flavonoids, and 9 iridoids were identified. EEuOL significantly reversed the loss of dopaminergic neurons and neural vasculature as well as reduced the numbers of apoptotic cells in the zebrafish brain in a concentration-dependent manner. Moreover, EEuOL relieved locomotor impairments in MPTP-modeled PD zebrafish. We also investigated the underlying mechanism and found that EEuOL may activate autophagy, contributing to α-synuclein degradation, therefore alleviate PD-like symptoms. Molecular docking simulation implied the interaction between autophagy regulators (Pink1, Beclin1, Ulk2, and Atg5) and phenolic acids of EEuOL, affirming the involvement of autophagy in EEuOL-exerted anti-PD action. The overall results indicated the anti-PD effect of EEuOL, opening a possibility to use the extract in PD treatment.

    更新日期:2019-12-30
  • Molecular Imprinted Polymers for the Controlled Extraction of Sinapic Acid from Aqueous Media
    Food Funct. (IF 3.241) Pub Date : 2019-12-30
    Roya Fathi Til; Mohammad Alizadeh-Khaledabad; Reza Mohammadi; Sajad Pirsa; Lee Wilson

    Several unique molecularly imprinted polymers (MIPs) were synthesized via a precipitation polymerization technique using 4-vinylpyridine as a functional monomer and ethylene glycol dimethacrylate as a cross-linker for selective extraction of sinapic acid from aqueous solution. Three sets of MIPs with different functional monomer: cross-linker molar ratios at 4:20; 8:20 and 8:32 were prepared along with the corresponding non-templated polymers (NIPs). The MIPs and NIPs were characterized by scanning electron microscopy, dynamic light scattering, N2 adsorption analysis, thermogravimetry, and FT-IR/solids 13C NMR spectroscopy. Batch rebinding and selectivity experiments were carried out to evaluate their adsorption performance, where the MIP prepared with a 4:20 monomer: cross-linker mole ratio had significantly higher affinity toward sinapic acid. Notably, all MIPs displayed greater molecular recognition with sinapic acid relative to the NIPs. Therefore, MIPs prepared by this approach represent promising advanced materials for the pre-concentration, isolation and enrichment of sinapic acid from complex food matrices or controlled removal from agricultural waste streams.

    更新日期:2019-12-30
  • Structural responses of kiwifruit allergen Act d 2 to thermal and electric field stresses based on molecular dynamics simulation and experiment
    Food Funct. (IF 3.241) Pub Date : 2019-12-28
    Jin Wang; Sai Kranthi Kumar Vanga; Vijaya G. S. Raghavan

    Kiwifruit is considered as the most common plant-based food causing allergic reactions after peanuts, soybeans, and wheat. These responses are triggered by proteins in kiwifruit. Modifying the structures of these allergenic proteins (epitopes) through external stresses may reduce their specific-binding capacity with antibodies resulting in the reduction of kiwifruit allergenicity. To provide a visual insight into the structural changes of Act d 2, we treated it with thermal force and oscillating electric field using molecular dynamics (MD) simulation. The results found that Act d 2 is a heat-stable protein, while the electric field combined with thermal stress significantly affected its secondary structure and snapshot of surface properties, which could result in conformational changes. The results obtained from the ELISA test found that the combination of thermal and electric field significantly reduced the Ig-E binding capacity of Act d 2 by 75.3%, accompanying obvious losses of alpha-helix and turn structures.

    更新日期:2019-12-29
  • High fat diet incorporated with meat proteins changes biomarkers of lipid metabolism, antioxidant activities, and serum metabolomic profile in Glrx1-/- mice
    Food Funct. (IF 3.241) Pub Date : 2019-12-27
    Muhammad Ijaz Ahmad; Muhammad Umair Ijaz; Muzhair Hussain; Iftikhar Ali; Noreen Mehmood; sultan mehmood siddiqi; Liu Concong; Di Zhao; Xinglian Xu; Guanghong Zhou; Chunbao Li

    Red and processed meat consumption has been associated with oxidative stress, diabetes and non-alcoholic fatty liver disease (NAFLD). This study was aimed to explore the effects of high-fat meat protein diets on potential metabolite biomarkers in Glrx1-/- mice, a well-documented mouse model to study NAFLD. Male Glrx1-/- mice were fed a Control diet with 12% energy (Kcal) from fat, a high-fat diet supplemented with casein (HFC) with 60% energy (Kcal) from fat, a high-fat diet supplemented with fish (HFF) or mutton proteins (HFM) for 12 weeks. The results of biochemical and histological analyses indicated that intake of HFM increased hepatic total cholesterol, triglycerides, serum alanine transaminase and aspartate transaminase, and macro- and micro-vesicular lipid droplets accumulation, which are accompanied by altered gene expression associated with lipid and cholesterol metabolism. Glrx1-/- mice fed HFF diet significantly ameliorated diet-induced NAFLD biomarkers compared to HFC and HFM diets. In addition, serum metabolome profiling identified metabolites specifically associated with lipid metabolism (lysophosphatidyl cholines and lysophosphatidyl ethanolamine), bile acids metabolism, sphingolipid and amino acid metabolism pathways. A HFM diet increased the abundances of LysoPC(15:0), LysoPC(16:0), LysoPC(17:0), LysoPC(20:1), LysoPE(18:2), LysoPE(22:0), LysoPE(20:6), O-arachidonoylglycidol, 12-ketodeoxycholic acid and sphinganine that are associated with NAFLD. The KEGG metabolic pathway of identified metabolites of high fat diets showed that the differential metabolites were associated with lipid metabolism, linoleic acid metabolism, amino acid metabolism, bile acid metabolism, sphingolipid metabolism, glycine and serine metabolism and glutathione metabolism pathways whereas HFF diet ameliorated NAFLD by modifying these pathways. These results provide potential metabolite biomarkers for NAFLD induced by HFM diet.

    更新日期:2019-12-27
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