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  • Advances and Applications of Stable Isotope Labeling-based Methods for Proteome Relative Quantitation
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-25
    Jianhui Liu; Yichu Shan; Yuan Zhou; Zhen Liang; Lihua Zhang; Yukui Zhang

    Proteins carry out numerous biological functions in living cells and form a dynamic system under various physiological and pathological conditions. Advances in proteome quantitation strategies have deepened our understanding of complex biological processes. Herein, we highlight stable isotope labeling-based quantitation methods and discuss the properties of each method, directed by the features of quantitation ions. Furthermore, the applications of such methods in quantifying a variety of protein properties, including post-translational modifications, subcellular localization, turnover rate, secretion and variation in multicellular environments, are also summarized.

    更新日期:2020-01-26
  • 更新日期:2020-01-22
  • Liquid chromatography above 20,000 PSI
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-21
    Matthew J. Sorensen; Brady G. Anderson; Robert T. Kennedy

    Continued improvements in HPLC have led to faster and more efficient separations than previously possible. One important aspect of these improvements has been the increase in instrument operating pressure and the advent of ultrahigh pressure LC (UHPLC). Commercial instrumentation is now capable of up to ∼ 20 kpsi, allowing fast and efficient separations with 5 – 15 cm columns packed with sub-2 μm particles. Home-built instruments have demonstrated the benefits of even further increases in instrument pressure. The focus of this review is on recent advancements and applications in liquid chromatography above 20 kpsi. We outline the theory and advantages of higher pressure and discuss instrument hardware and design capable of withstanding 20 kpsi or greater. We also overview column packing procedures and stationary phase considerations for HPLC above 20 kpsi, and lastly highlight a few recent applications of ultrahigh pressure instruments for the analysis of complex mixtures.

    更新日期:2020-01-22
  • Recent insights into functionalized electrospun nanofibrous films in chemo-/bio-sensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-18
    Tong Yang; Lei Zhan; Cheng Zhi Huang
    更新日期:2020-01-21
  • 更新日期:2020-01-21
  • Frontiers In Electrochemical Enzyme Based Biosensors For Food And Drug Analysis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-17
    Sevinc Kurbanoglu; Cem Erkmen; Bengi Uslu

    Nowadays, the development of various biosensors as a new generation of analytical instruments is one of the most promising research fields of analytical biology. Among all types of biosensors, enzyme based biosensors have an interesting property, the inherent inhibition phenomena given the enzyme-substrate complex formation. Over the past years, electrochemical enzyme based biosensors have emerged as simple, rapid and ultra-sensitive devices for determination or detection of different compounds in the drugs and food samples. In this review, general views to enzymes related with their history, classification, immobilization, and inhibition information is presented to researchers. Then, a detailed description is provided for enzyme based and electrochemical enzyme based biosensors. Finally, some selected electrochemical enzyme based biosensor studies developed for food and drug analysis are summarized and tabulated.

    更新日期:2020-01-17
  • Chiral Capillary Electrophoresis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-10
    Samuel Bernardo-Bermejo; Elena Sánchez-López; María Castro-Puyana; María Luisa Marina

    The implications of chirality in different environments are already well known and reported extensively in the literature. Capillary Electrophoresis, a separation technique that only requires few nanoliters of sample, has demonstrated its potential for chiral analysis in the past years. The aim of this article is to provide an overview on the fundamentals and characteristics of Chiral Capillary Electrophoresis as well as the main advances and trends in this topic. Special attention is paid to the most recent technological and methodological developments achieved mainly in the most employed separation mode (Electrokinetic Cromatography). The most noteworthy and recent applications reported on the enantiomeric separation and determination of compounds in pharmaceutical, food, biomedical, environmental or forensic samples will also be critically overviewed. The characteristics of the developed methodologies will be detailed in Tables and future trends will also be discussed.

    更新日期:2020-01-11
  • Recent advances in cancer early detection and diagnosis: role of nucleic acid based aptasensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-09
    Eman M. Hassan; Maria C. DeRosa

    Cancer is a complex disease of worldwide concern. Along with an improvement in outcomes, early and sensitive detection of cancer and cancer biomarkers can facilitate an understanding of cancer progression and aid in the development of effective treatment methods. Among the many detection methods available, aptasensors have shown great promise in recognition and identification of cancer cells and their biomarkers. In this review, we summarize the recent advances in optical and microfluidic aptasensors for early detection of cancer, as well as the recent developments in aptamer-based platforms for cancer exosome detection. The aptamer production process and their characterizations are initially introduced, and then the latest optical aptasensors for cancer cell recognition and detection are summarized. Microfluidic aptamer-based technologies for sensitive biomarker and cancer cell detection are then discussed. After that, the detection of cancer exosomes using recent fluorescent, colorimetric, and other approaches is elaborated. Finally, we provide a perspective on the future of aptasensors for cancer detection.

    更新日期:2020-01-09
  • Gas chromatographic separation of enantiomers on novel chiral stationary phases
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-09
    Sheng-Ming Xie; Xue-Xian Chen; Jun-Hui Zhang; Li-Ming Yuan

    Chiral capillary gas chromatography has been widely applied to separate volatile and thermally stable enantiomers due to its advantages of simplicity, high efficiency, fast analysis, good sensitivity, and absence of liquid mobile phases. In the first part of this article, recent research progress (from 2010 to the present) concerning newly developed chiral stationary phases based on cyclofructan derivatives and chiral porous materials, including metal-organic frameworks, covalent organic frameworks, inorganic mesoporous silicas, and molecular cages for use in gas chromatography, and the synthetic strategies for obtaining chiral porous materials, was reviewed. Finally, we discussed in detail the separation performances and chiral recognition mechanisms of novel chiral recognition materials coated on capillary columns towards various types of enantiomers, namely (1) amino acid derivatives; (2) alcohols, amines, and amino alcohols; (3) organic acids; (4) aldehydes and ketones; (5) ethers and epoxides, and (6) esters and other enantiomers.

    更新日期:2020-01-09
  • Recognition and Separation of enantiomers based on functionalized magnetic nanomaterials
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-09
    Xiaojuan Deng; Wenbin Li; Yi Wang; Guosheng Ding

    Enantioselective separation is significantly important for the study of pharmacology and biology, but it is still a big challenge because of the identical physical and chemical properties of enantiomers in an achiral environment. Magnetic nanomaterials have unique properties such as extremely large surface area per volume substance and strong magnetic response. It is very easy for them to be isolated from complicated matrixes, only by applying external magnets. The possibilities for surface functionalization make them exciting candidates for enantioseparation. Functionalized magnetic nanomaterials are unique tools for the simple and rapid enantioseparation compared with traditional chiral stationary phase. In this review, various magnetic nanomaterials modified with specific chiral selectors are reviewed in this manuscript. The prepared functional magnetic materials are typically used as chiral adsorbents for enantioseparation applications, formatted by methods of dispersive solid phase extraction, enantioselective crystallization, or column and microchip separation.

    更新日期:2020-01-09
  • Plasmonics-attended NSET and PRET for analytical applications
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-08
    Peng Fei Gao; Yuan Fang Li; Cheng Zhi Huang
    更新日期:2020-01-09
  • Chemical separation and characterization of complex samples with herbal medicine
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-07
    Qing Fu; Yanxiong Ke; Dasen Jiang; Yu Jin

    Herbal medicine has attracted great interest throughout the world. However, separation and characterization of components in herbs is still a challenge. Advanced techniques involved in this task, mainly including hydrophilic interaction liquid chromatography, supercritical fluid chromatography, two-dimensional chromatography and preparative chromatography, have showing an ongoing development in recent years. In this review, in addition to summarizing their progress and applications in herbal medicine in the last five years, their combination with mass spectrometry, activity screening or other techniques were introduced in detail.

    更新日期:2020-01-07
  • Deep learning and artificial intelligence methods for Raman and surface-enhanced Raman scattering
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-07
    Félix Lussier; Vincent Thibault; Benjamin Charron; Gregory Q. Wallace; Jean-Francois Masson
    更新日期:2020-01-07
  • Recent analytical advancements in microfluidics using chemiluminescence detection systems for food analysis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-07
    Baqia Al Mughairy; Haider A.J. Al-Lawati

    Nowadays, the analysis of food is of high concern in terms of quality and safety. Microfluidic chemiluminescence have been devoted in vast food analyses proffering several merits and over performing different conventional methods in terms of sensitivity and selectivity. The ease of coupling with different separation techniques facilitated a comprehensive and adequate food analyses. This review explicitly focuses on the critical advancements in microfluidic chemiluminescence exploitation in food analyses based on the different pre-separation techniques utilized and the modes of coupling. Different dedications by microfluidic chemiluminescence systems including lab-on-a chip, μTAS, lab-on-a paper (μPADs) and lab -on- a cloth articles will be discussed and inspected.

    更新日期:2020-01-07
  • 更新日期:2020-01-04
  • Recent advances in potentiometric biosensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-03
    Jiawang Ding; Wei Qin

    Potentiometry based on ion-selective electrodes (ISEs) has been injected with new vigor and gone through a renaissance with the improvements in the detection limits and selectivities of ISEs, the introduction of new materials, new sensing concepts (from conventional potentiometry to dynamic electrochemistry approaches), and deeper theoretical understanding and modelling of the potentiometric responses of ISEs. The new breakthroughs encourage innovations in ion sensing and biosensing applications. Moreover, with the introduction of new bioreceptors, such as enzymes, antibodies, aptamers, pepetides, versatile sensing protocols have been designed for a broad range of different target molecules by using ISEs as powerful transducers. This paper reviews the recent trends in potentiometric biosensors. Their applications in biosensing of metal ions, small molecules, DNA, proteins, bacteria and toxicities have been discussed. This review provides the outlook of the potentiometric biosensing based on the integration of potentiometric ISEs with new materials and emerging techniques.

    更新日期:2020-01-04
  • Recent Advances in Differentiation of Isomers by Ion Mobility Mass Spectrometry
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-02
    Qian Wu; Jian-Ying Wang; Dong-Qi Han; Zhong-Ping Yao

    Differentiation of isomers is an important analytical task in many fields, e.g., chemistry, biology, pharmaceutical science, medicine, environmental science, food science. Ion mobility mass spectrometry (IM-MS) can resolve isobaric ions based on their shape and size and measure the collision cross section (CCS) values of ions to provide information on their intrinsic structures, and is thus a very promising tool for differentiation of isomers. In this review, the applications of IM-MS in differentiation of various isomers including glycans, lipids, amino acids, peptides, proteins, as well as metabolite isomers, chiral isomers, etc. are summarized. Prospects for further development of IM-MS for differentiation of isomers, e.g., improved resolving powers of IM-MS instruments, expanded CCS database and coupling of IM-MS with computational approaches, are also discussed.

    更新日期:2020-01-02
  • Design of smart chemical 'tongue' sensor arrays for pattern-recognition-based biochemical sensing applications
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-30
    Zi-Han Chen; Qian-Xi Fan; Xin-Yue Han; Guoyue Shi; Min Zhang

    Conventional molecular recognition was tightly related to the development of analyte-specific sensor. However, all most every biochemical process are not decided on only one factor. When several factors were imported simultaneously, the established sensor could be seriously interfered and gave the wrong response. In this regard, chemical tongue-mimic sensor array was promoted to recognize a set of analytes, which concerned each component of mixtures. This review, focused on the development of chemical “tongue” sensor arrays from recent years, and gave enlightening examples about how to design integrated sensor system, how to choose the responsive signals and explain the mechanism of each used. As for applications, this review concluded the status of what chemical or biochemical species could be discriminated, what the advantages or drawbacks they had and the novel mechanism they used. In the last, this review also pointed out the limitations and future of related field.

    更新日期:2019-12-31
  • Design Strategies of AuNPs-based Nucleic Acid Colorimetric Biosensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-30
    Tao Yang; Zewei Luo; Yonghui Tian; Cheng Qian; Yixiang Duan

    Nucleic acids are important biomarkers related to diseases of cancer, infectious, and genetic disorders. As a kind of indicator, gold nanoparticles (AuNPs) can generate colored signals from red to blue, which enables convenient signal readout. Based on the inherent optical properties, AuNPs are frequently used to fabricate colorimetric biosensors. Crosslinking colorimetry of polyvalent DNA-functionalized AuNPs and salt-induced colorimetry of unmodified AuNPs are two basic AuNPs-based colorimetric schemes. In order to improve such sensors sensitivity or add some specific functions, some amplification reactions or functionalized nanomaterials need to be introduced. Therefore, in this review, colorimetric biosensors with different functions and features are discussed, together with their advantages and limitations. By digging into the profound relationship between the various methods and strategies in this field, this review aims to provide a comprehensive reference outlining the general design strategies for constructing AuNPs-based nucleic acid colorimetric biosensors.

    更新日期:2019-12-30
  • Selection and Applications of Synthetic Functional DNAs for Bacterial Detection
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-30
    Erin M. McConnell; Devon Morrison; Maria Alejandra Rey Rincon; Bruno J. Salena; Yingfu Li

    Early detection of an infectious bacterium is essential to preventing a potential outbreak or minimizing the impact of an active infection. Although prevailing methods are effective in many aspects, they suffer from being complex, expensive or insensitive. These drawbacks prevent their applications by the general public or under resource-limited settings. Therefore, developing rapid, accurate, inexpensive and sensitive methods for pathogenic bacterial detection remains a major research focus in the global research community. In recent years, functional nucleic acids, particularly DNA aptamers and DNAzymes, have been increasingly examined as the recognition element for bacterial biomarkers and whole bacterial cells. Significant efforts have also been made to utilize these affinity probes for the design of simple and cost-effective biosensors for bacterial detection. This review highlights the key achievements in this area, identifies important challenges, and provides thoughts on future directions.

    更新日期:2019-12-30
  • Chemical sensor platforms based on fluorescence resonance energy transfer (FRET) and 2D materials
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-29
    Neema P. M; Ann Mary Tomy; Jobin Cyriac

    Enduring interests in two dimensional (2D) layered materials are attributed to their unique physicochemical properties. They are now extensively used in fluorescence-based chemical sensors as a quencher or as a fluorophore. In this review, we discuss recent progress in the field of chemical sensors that operate through fluorescence resonance energy transfer (FRET) in association with 2D materials. With a short introduction about the 2D materials, in general, we examine the luminescence properties of important 2D materials such as graphene, graphene oxide (GO), MoS2 and WS2. Various FRET-based sensors of these materials are presented after classifying them into FRET acceptors and FRET donors.

    更新日期:2019-12-29
  • Saliva sampling: methods and devices. An overview
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-28
    Francesca G. Bellagambi; Tommaso Lomonaco; Pietro Salvo; Federico Vivaldi; Marie Hangouët; Silvia Ghimenti; Denise Biagini; Fabio Di Francesco; Roger Fuoco; Abdelhamid Errachid

    The continuous exchange of chemicals with blood and the non-invasive collection make saliva an interesting specimen for clinical applications, from the detection of biomarkers to the new –omic sciences in medicine. However, saliva sampling is challenging because the suitability of the collection method for the analyte of interest is either poorly investigated or, more often, not mentioned at all in most publications. This review reports a critical evaluation of the most common procedures for saliva sampling and a description of the off-the-shelf sampling devices. Their suitability for bioanalytical applications and salivary biomarkers detection, e.g. representativeness of the sample, sampling feasibility, analyte recovery, and sample amount, is discussed.

    更新日期:2019-12-29
  • Recent advances in the bioanalytical and biomedical applications of DNA-templated silver nanoclusters
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-27
    Jiaqi Xu; Xuanmeng Zhu; Xi Zhou; Farjana Yeasmin Khusbu; Changbei Ma

    Silver nanoclusters stabilized with DNA scaffolds, with excellent physical and chemical properties, have emerged as a versatile tool in biomedical systems. Silver nanoclusters as a late-model nanomaterial with a small size less than 2 nm shows good stability and strong fluorescence. By using DNA with diverse sequence and structures, soluble DNA-templated silver nanoclusters are facilely prepared. They possess tunable fluorescence emission and are suitable with multifunctional designs. Extensive efforts have been put into the application potential of this biocompatible material for biosensing, bioimaging and therapy. Here we are committed to outline the recent advances of DNA-templated silver nanoclusters in biomedical application. The future prospect and challenges are then discussed considering rising progress. Though there is still a long way for illuminating the mechanism behind traits and conducting further clinical trials, we believe DNA-templated silver nanoclusters’ unprecedented unique properties will finally benefit medical progress.

    更新日期:2019-12-27
  • Pure water as a mobile phase in liquid chromatography techniques
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Mikołaj Dembek; Szymon Bocian

    Liquid chromatography is a technique that is gaining increasing interest and application, for example in the pharmaceutical industry. At the same time as the increase in the number of analyses performed, the amount of organic waste produced while working with high-performance liquid chromatography (HPLC) apparatus is growing. Therefore, new methods and materials are being searched for to achieve the so-called "green" chromatography. In the following review we describe one of them, specifically the replacement of harmful organic solvents such as acetonitrile, methanol or isopropanol, with pure water used as the sole component of the mobile phase. In order to achieve a single component mobile phase, different methods or materials are used: the use of elevated temperature, the selection of new stationary phases, the utilization of changes in the properties of the stationary phase while using a highly polar eluent.

    更新日期:2019-12-23
  • 更新日期:2019-12-23
  • FRET-based Nucleic Acid Probes: Basic Designs and Applications in Bioimaging
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Ke Quan; Cuiping Yi; Xiaohai Yang; Xiaoxiao He; Jin Huang; Kemin Wang

    Fluorescence imaging has emerged as one of the most robust techniques for noninvasive、real-time visualizing of biomolecules from in vitro to in vivo. Due to their high selectivity and sensitivity, ease of design and synthesis, suitability for structural modification, and minimum interference with living systems, FRET-based nucleic acid probes have been found instrumental for revealing the temporal and spatial resolution of biomolecules and biological process. In this review, we first give a brief introduction to the basic theory about FRET and fluorescent materials available for FRET, then particular attention is focused on typical designs of FRET-based nucleic acid probes and overview of the recent bioimaging applications of FRET-based nucleic acid probes from live cells to small animals. Furthermore, the challenges and prospects on future research directions of this promising field are also included.

    更新日期:2019-12-23
  • Current overview and perspectives on carbon-based (bio)sensors for carbamate pesticides electroanalysis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Thiago M.B.F. Oliveira; Francisco W.P. Ribeiro; Camila P. Sousa; Giancarlo R. Salazar-Banda; Pedro de Lima-Neto; Adriana N. Correia; Simone Morais

    Carbamate pesticides (CBMs) are esters and thioesters derived from carbamic acid, which are widely used to increase agricultural productivity and to protect human and animal health from insect-vector-mediated diseases. However, they can be highly toxic when not appropriately applied. Electrochemical carbon-based sensors and biosensors gather a plethora of advantages for reliable, low cost, rapid and even in loco quality control and surveillance of CBMs levels in all systems related to environmental and human health. Thus, this review presents a broad overview of the state-of-art of design and applications of these (bio)sensors for CBMs electroanalysis in environmental, food and biological matrices with emphasis in the role of the carbon (nano)materials. The latest advances and trends but also the main (bio)sensors limitations and research gaps are highlighted. Highly selective, robust and novel carbon-based (bio)sensors with multiplexing CBMs capability are envisaged to emerge shortly for in-field applicability.

    更新日期:2019-12-23
  • Analysis of chiral drugs in environmental matrices: current knowledge and trends in environmental, biodegradation and forensic fields
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Ana Rita L. Ribeiro; Alexandra S. Maia; Cláudia Ribeiro; Maria Elizabeth Tiritan

    The challenge to develop enantioselective analytical methods to quantify residues of chiral drugs (CDs) in environmental matrices is an actual and imperative issue. It is well known that enantiomers may differ in their biological activities; nevertheless, most environmental analytical methods still ignore the stereochemistry and the discrimination of the enantiomers. The knowledge about their occurrence and ecological impact in biota is crucial for an accurate risk assessment. This critical review highlights the importance of analysing CDs in environmental matrices for various applications, emphasizing methodological trends. Chromatographic methodologies are the most used and include liquid (LC), gas (GC), supercritical fluid (SFC) and capillary electrophoresis. LC is still the most widely used though trends for greener approaches and the development of GC and SFC methods are also future directions. Some of the most recent applications, namely for environmental monitoring of surface and wastewater, biodegradation studies and forensic analysis, are presented and critically discussed.

    更新日期:2019-12-23
  • Multifunctional aptasensors based on mesoporous silica nanoparticles as an efficient platform for bioanalytical applications: Recent Advances
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Houman Kholafazad Kordasht; Mahdiyeh Pazhuhi; Paria Pashazadeh-Panahi; Mohammad Hasanzadeh; Nasrin shadjou
    更新日期:2019-12-23
  • Research progress on chemiluminescence immunoassay combined with novel technologies
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Qin Xiao; Chunxia Xu

    Chemiluminescence immunoassay (CLIA) has been greatly developed in the past several decades due to its good sensitivity and specificity. Nowadays, CLIA has been highly improved with novel nanomaterials and newly-developed technologies. The advancement of CLIA combined with relevant technologies are reviewed in the paper, including enhanced chemiluminescent, antibody preparation, aptamer selection, nanomaterials assisted CLIA, and CLIA coupling with newly-developed technologies. Finally, some critical challenges are discussed and the future development direction is prospected in CLIA. The review will be of great significance for CLIA basic research and practical applications in the fields of biomedical diagnostics, food and drug testing, environmental monitoring, and other fields.

    更新日期:2019-12-23
  • Sample preparation and extraction methods for pesticides in aquatic environments: A review
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-18
    Maryam Nasiri; Hossein Ahmadzadeh; Amirhassan Amiri

    Pesticides are the most hazardous environmental pollutants due to their stability, mobility and long-term effects on living organisms. Their existence in sources of drinking water, essential to life, is a specific danger to living organisms. In water, these compounds may metamorphose to produce substances with even greater toxicity. Pesticide levels in diverse environments are generally below the limit of detection for many analytical instruments, so it is essential to isolate and then enrich pesticides from the complex matrix to decrease the interference effects in order to obtain a reliable signal. The main objective of this review is to evaluate the new sample preparation and extraction methods for the analysis of pesticides in various water samples.

    更新日期:2019-12-19
  • Construction principles to modify responsive performance of fluorescent receptors: from background clearance to signal enhancement
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-18
    Peng Zhang; Qian Zhang; Lianxiao Zhang; Hua Zhong; Caifeng Ding

    There presents a rapid development for fluorescent sensory systems in recent years, appended with multiple functions, including organelle targeting, near-infrared emission, photothermal property and so forth. These in-depth studies extend the application area undoubtedly, but an excellent responsive behavior still needs a precise control. This Review focuses on the chemical modulation for responsive performance, which is discussed mainly in two parts, improvement of signal-noise ratio and signal amplification strategy. In addition to multifaceted literatures to illustrate the ratiometric strategy, comments on background-free platform are summarized elaborately, which propose a total clearance in background by in situ reactions. As for proposal to modify the responsive spectra, the control on chemical equilibrium, introduction of an additional “amplifier” and self-assembly-featured spectra manipulated by analytes are elucidated in detail, aiming to provide a straightforward pathway to constitute a set of well-behaved receptors integrating analytes-sensing with dye-distribution, avoiding the increasing demands for a complex probe.

    更新日期:2019-12-19
  • Invited Review: Recent applications of FRET-based multiplexed techniques
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-17
    Anisa Kaur; Soma Dhakal

    Fluorescence resonance energy transfer (FRET) has become a powerful tool for visualizing molecular interactions, single-molecule conformational dynamics, binding, and other molecular signaling events. However, until recently, FRET spectroscopy/microscopy has been mainly used for monitoring single events. Recent pursuits on simultaneous detection and analysis of multiple analytes have led to the development of various FRET-based multiplexed techniques. The concomitant increase in the experimental complexity and data analysis due to the use of multiple donors/acceptor pairs and multiple excitation sources have proven to be the major challenges for rapid expansion of these techniques. In this review, we will focus on recent applications of FRET-based multiplexed techniques using coupled chromophore platforms composed of organic dyes, quantum dots (QDs), lanthanides, and fluorescent proteins, and discuss some of the current challenges that need to be addressed to further expand their applications in this new exciting area.

    更新日期:2019-12-18
  • Recent advances in preparation and applications of monolithic chiral stationary phases
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-13
    Jialiang Guo; Qiqin Wang; Dongsheng Xu; Jacques Crommen; Zhengjin Jiang

    Chiral separation remains a very important topic in different fields of analytical chemistry such as pharmaceutical, agrochemical, environmental and food analysis. Over the last 20 years, various chiral monoliths, based on chiral selectors such as cyclodextrins and their derivatives, polysaccharide derivatives, proteins, antibiotics, chiral ligands, cinchona alkaloids and amino acid derivatives, as well as molecularly imprinted polymers, have been developed for the enantioseparation of a wide range of analytes. The present review summarizes the recent developments of monolithic chiral stationary phases (CSPs) with particular emphasis on their classification and preparation. The major challenges and future prospects of monolithic CSPs are also briefly discussed.

    更新日期:2019-12-17
  • Advances in biosensing technologies for analysis of cancer-derived exosomes
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-13
    Huiying Xu; Bang-Ce Ye

    Exosomes play an important role in intercellular communication that contribute to the interaction of cancer cells with the tumor microenvironment. The original features endow tumor-derived exosomes with distinctive opportunities to serve as sensitive biomarkers for identifying early-stage tumors and monitoring of therapeutic response non-invasively. Conventional detection techniques face a number of drawbacks including sensitivity and reproducibility, therefore considerable efforts have been made to establish reliable biosensors for exosomes analysis. This review summarizes recent progress in exosomes quantification techniques, mainly focused on novel molecular recognition methods and signal transduction strategies. Furthermore, we offer a perspective of exosomes research towards the development innovative concepts on future applications.

    更新日期:2019-12-17
  • AIE luminogens as fluorescent bioprobes
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-06
    Guangle Niu, Ruoyao Zhang, Xiujuan Shi, Hojeong Park, Sheng Xie, Ryan T.K. Kwok, Jacky W.Y. Lam, Ben Zhong Tang
    更新日期:2019-12-06
  • Current advances in the detection of neurotransmitters by nanomaterials: An update
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-06
    Shiva Kumar Arumugasamy, Gayathri Chellasamy, Sivalingam Gopi, Saravanan Govindaraju, Kyusik Yun

    Neurotransmitters (NTs) are chemical messengers in the synaptic transmission process. NTs play important roles in human activities, and imbalanced NTs levels in the body lead to severe diseases such as Alzheimer’s and Parkinson’s diseases. The accurate diagnosis of NTs imbalances has been identified only after patient death through identifying plaques in the brain. Hence, the diagnosis and monitoring of NTs concentrations is important for avoiding serious illnesses. Many researchers have explored the use of robust diagnostic tools with various nanomaterials and techniques. Herein, we investigated the mechanism of reactions between NTs and nanomaterials, the role of nanomaterials in diagnostic tools and the different techniques used in the accurate detection of NTs. Furthermore, we discussed the simultaneous detection of NTs in a single sensing channel, detection in biological samples and future perspectives for nanomaterials in NTs detection.

    更新日期:2019-12-06
  • Recent advances in electrochemiluminescence-based simultaneous detection of multiple targets
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-05
    Wanxue Lv, Haochen Ye, Zhiqin Yuan, Xuejiao Liu, Xu Chen, Wensheng Yang
    更新日期:2019-12-05
  • Nano-impact Electrochemistry: Analysis of Single Bioentities
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-05
    Jian-Hua Zhang, Yi-Ge Zhou

    A central challenge of sensor technology is that the sensitivity of analytical detection is required to reach a single analyte entity level, whether it is a molecule, a cell or a nanoparticle. The emergence of nano-impact electrochemistry (NIE) allows in situ detection of single analyte entity one at a time with simplicity, fast response and high throughput. NIE method was originally designed to characterize physical and chemical properties of the corresponding single nanoparticles, and has been later extended into the field of bio-analysis, enabling better understanding of biological heterogeneity and providing new route for developing new diagnostic devices for quantifying biological analytes. A wide range of biological species including DNA, RNA, enzymes, bacteria, vesicles and cells has been already studied using NIE method so far. In this review, we first summarize the basic principles of NIE for bio-analyte detection and then elaborate NIE based bio-analysis categorized by analyte types. Finally, we give an outlook on the future prospects of this field.

    更新日期:2019-12-05
  • A comprehensive approach to the analysis of antibiotic-metal complexes
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-05
    Sandra Ramotowska, Małgorzata Wysocka, Jakub Brzeski, Agnieszka Chylewska, Mariusz Makowski
    更新日期:2019-12-05
  • Arsenic speciation analysis: A review with an emphasis on chromatographic separations
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-05
    Michael S. Reid, Karen S. Hoy, Jordan R.M. Schofield, Jagdeesh S. Uppal, Yanwen Lin, Xiufen Lu, Hanyong Peng, X. Chris Le

    More than 100 different arsenic species of diverse characteristics are present in the environment and biological systems. The identification and quantification of individual arsenic species are critical to understanding the distribution, environmental fate and behaviour, metabolism, and toxicity of arsenic species. This review summarizes sample preparation, separation, detection, and method validation for arsenic speciation analysis. An emphasis is placed on chromatographic separation techniques, relating the physicochemical properties of arsenic species to their efficient separation. Anion exchange, cation exchange, reversed-phase, ion pair, and size exclusion chromatography are useful to separate various arsenic species. Recent research has explored hydrophilic interaction liquid chromatography (HILIC), multiple separation mechanisms, and testing of fluorophenyl and graphene oxide stationary phases for the separation of arsenic species. Sample preparation, extraction of arsenic species, recovery of arsenic species from separation columns, and method validation are discussed in light of their importance to the integrity and accuracy of speciation analysis.

    更新日期:2019-12-05
  • Ultraspecific analyte detection by direct kinetic fingerprinting of single molecules
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-04
    Tanmay Chatterjee, Zi Li, Kunal Khanna, Karen Montoya, Muneesh Tewari, Nils G. Walter, Alexander Johnson-Buck

    The detection and quantification of biomarkers have numerous applications in biological research and medicine. The most widely used methods to detect nucleic acids require amplification via the polymerase chain reaction (PCR). However, errors arising from the imperfect copying fidelity of DNA polymerases, limited specificity of primers, and heat-induced damage reduce the specificity of PCR-based methods, particularly for single-nucleotide variants. Furthermore, not all analytes can be amplified efficiently. While amplification-free methods avoid these pitfalls, the specificity of most such methods is strictly constrained by probe binding thermodynamics, which for example hampers detection of rare somatic mutations. In contrast, single-molecule recognition through equilibrium Poisson sampling (SiMREPS) provides ultraspecific detection with single-molecule and single-nucleotide sensitivity by monitoring the repetitive interactions of a fluorescent probe with surface-immobilized targets. In this review, we discuss SiMREPS in comparison with other analytical approaches, and describe its utility in quantifying a range of nucleic acids and other analytes.

    更新日期:2019-12-04
  • Enantioseparation using helical polyacetylene derivatives
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-04
    Chunhong Zhang, Lijia Liu, Yoshio Okamoto

    In the past few decades, the enantioseparations techniques have been quickly developed in order to meet the needs of single enantiomers in many fields. Polyacetylene derivatives have attracted considerable attention due to their rigid conjugated backbone with high stereoregularity that can be readily induced into a regular helical conformation by the chiral bias covalently or non-covalently bonding to the backbone. Macromolecular helicity plays an important role in enhancing the enantioseparation ability, and the controllable and stereoregular helical structure of the polyacetylenes affords them a wide variety of potential applications, which are promising candidates for enantioseparation. In this review, the discovery and development of helical polyacetylene derivatives and the enantioseparations using helical polyacetylene derivatives as chiral stationary phases (CSPs) for high-performance liquid chromatography (HPLC), optical resolution membranes, enantioselective adsorbents and chiral additives for enantioselective crystallization will be described.

    更新日期:2019-12-04
  • Recent Advances in Background-Free Raman Scattering for Bioanalysis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-04
    Xuehui Liu, Xiaoman Liu, Pengfei Rong, Dingbin Liu

    With the characteristics of intrinsically free from photobleaching, blinking, and self-quenching, Raman microscopy has become a powerful platform in detecting molecular vibrations for diverse applications. In particular, Raman spectroscopy has proven very useful for bioanalysis but suffers from several challenges such as poor sensitivity, spectral overlapping, and background interference. In this review, we would like to introduce two kinds of high-fidelity imaging strategies, including background-free surface-enhanced Raman scattering (SERS) and stimulated Raman scattering (SRS) that are capable of significantly enhancing detection sensitivity and spectral resolution while minimizing background interference simultaneously. Recent advances in background-free SERS and SRS and their applications in biological detection and cellular imaging are summarized and discussed.

    更新日期:2019-12-04
  • Chiral analysis in food science
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-03
    Gerardo Alvarez-Rivera, Mónica Bueno, Diego Ballesteros-Vivas, Alejandro Cifuentes

    Determination of enantiomeric ratios in food and beverages is a research area of great interest because enantiomers can exhibit different biological activities. Chiral analysis can provide valuable information about food safety, bioactivity, quality and traceability. In this work, the potential of separation techniques such as liquid chromatography, gas chromatography, supercritical fluid chromatography and capillary electrophoresis for chiral analysis of food is reviewed. The analysis of enantiomers based on other chiral methodologies such as countercurrent chromatography, sensors, biosensors and direct mass spectrometry (MS) analysis is also included. The present review article provides an updated perspective on the importance of chiral analysis in food matrices and beverages covering the period from February 2017 to July 2019, following the previous work published in this journal on the same topic (G. D'Orazio et al., TrAC-Trends in Analytical Chemistry 96 (2017) 151-171).

    更新日期:2019-12-04
  • Non-linear mass transport in confined nanofluidic devices for label-free bioanalysis/sensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-03
    Fei-Fei Liu, Xiao-Ping Zhao, Bin Kang, Xing-Hua Xia, Chen Wang

    In the past decade, a significant growth of research in nanofluidics is achieved. The electric double layer (EDL) overlapped in the nanochannel/nanopores results in non-linear mass transport. To understand the principle of non-linear mass transport in nanofluidics can help us to control and manipulate mass transfer and find applications in varied fields such as energy conversion and storage, gas separation, and bioanalysis/sensor. In this review, we first introduce the fabrication of nanofluidic devices, then demonstrate the origin mechanism of non-linear mass transport, among which the size and charge effect of nanofluidics are explained. Following that the influential factors regarding the non-linear mass transport behaviors are discussed in detail. After full understanding of the fundamental of non-linear mass transport in nanofluidics, the recent progress in bioanalytical applications based on nanofluidic non-linear transport are highlighted. Finally, the perspectives for future directions and development are discussed for this special research field.

    更新日期:2019-12-04
  • Chiral Mass Spectrometry: An Overview
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-03
    Dong-Qi Han, Zhong-Ping Yao

    Chiral analysis by mass spectrometry has attracted much attention due to its advantages in speed, sensitivity and specificity. In this review, recent advances in chiral analysis by mass spectrometry are summarized, with the methods based on tandem mass spectrometry (e.g., kinetic method, chiral recognition ratio method and photodissociation mass spectrometry method) and ion mobility mass spectrometry highlighted. Different methods are compared, and the limitations of the current studies and the prospects of chiral mass spectrometry are discussed.

    更新日期:2019-12-03
  • Resonance energy transfer to gold nanoparticles: NSET defeats FRET
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-02
    Chi Chen, Niko Hildebrandt

    Gold nanoparticles (AuNPs) have been widely applied as highly efficient luminescence quenchers in combination with a large variety of donor fluorophores. While the application of such systems in optical biosensing has resulted in significant advances of biological, biochemical, and biophysical research, the underlying energy transfer mechanism has been much less investigated. In this review, we discuss the differences between Förster resonance energy transfer (FRET) and nanosurface energy transfer (NSET), give an overview of the literature concerning the assignment of FRET or NSET to AuNP-based luminescence quenching, show why the development of improved NSET models and their application to experimental data provided strong arguments in favor of the NSET formalism, and discuss applications of NSET in biosensing, molecular rulers, and molecular tension probes. Taking into account the existing literature, the NSET formalism, including size and wavelength-dependent issues, is superior to FRET theory for a general description of energy transfer to AuNPs.

    更新日期:2019-12-03
  • Aptamer-conjugated mesoporous silica nanoparticles for simultaneous imaging and therapy of cancer
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-02
    Somayeh Vandghanooni, Jaleh Barar, Morteza Eskandani, Yadollah Omidi

    Mesoporous silica nanoparticles (MSNs) possess wide requirements for successful drug delivery, due to their superior physicochemical properties such as the well-organized porous structure, the higher volume of pore and surface area, and low in vivo toxicity. Once armed with homing devices such as aptamers (Aps), MSNs can actively be delivered to target sites with minimized off-target side effects and maximized therapeutic effects with a lower dose of the drug. Unique features of Aps (e.g., small size, high stability, structure flexibility, and low or no immunogenicity) make them robust targeting ligands for the development of various DDSs. Considerable researches have been performed on the advancement of different Aps-decorated MSNs for enhanced cancer therapy/imaging with substantial successes even though several crucial issues still remain to be addressed. This review focuses on the development and applications of Aps-armed MSNs as robust targeted DDSs for simultaneous cancer diagnosis and therapy.

    更新日期:2019-12-02
  • Cellular fluorescence imaging based on resonance energy transfer
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-01
    Wei Zhang, Xiaolei Liu, Ping Li, Wen Zhang, Hui Wang, Bo Tang

    Fluorescence imaging of intracellular active molecules plays an important role in understanding the occurrence and development of diseases. Resonance energy transfer (RET) has been recognized as a powerful technology in the design of fluorescence probes for biological imaging. Here, we summarize the novel approaches to fabrication of RET-based fluorescent probes systems such as the organic molecular fluorescent probe, nano fluorescent probe, protein probe and their recent applications in cellular fluorescence imaging.

    更新日期:2019-12-02
  • Semiconductor Quantum Dot FRET: Untangling Energy Transfer Mechanisms in Bioanalytical Assays
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-30
    Preston T. Snee

    Since their development at the turn of the millennium, bright and water-soluble semiconductor quantum dots (QDs) have been applied in analytical chemistry. QDs serve as excellent platforms for ratiometric sensing of biologicals via analyte-dependent manipulation of dipolar energy transfer. Several examples are provided, such as sandwich assays comprised of lanthanide donors and QD acceptors and highly multiplexed sensing schemes that have been realized due to QDs’ supermolecular sizes. However, QDs do not conform to the standard Förster energy transfer mechanism. For example, QDs are excellent donors but poor acceptors when coupled to organic dyes; yet, the same is not true for inorganic phosphors. Furthermore, QDs may reduce or oxidize substrates while engaging in dipolar energy transfer with them. Many of these observations have yet to be explained. Fortunately, optical methods are being developed to untangle these competing mechanisms, which will lead to a complete understanding of QD energy transfer processes.

    更新日期:2019-11-30
  • Advances in Imaging-Assisted Sensing Techniques for Heavy Metals in Water: Trends, Challenges, and Opportunities
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-29
    Rishabh Jain, Anupma Thakur, Pahuljot Kaur, Ki-Hyun Kim, Pooja Devi
    更新日期:2019-11-30
  • Design Strategies for Fluorescent Proteins/Mimics and Their Applications in Biosensing and Bioimaging
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-29
    Honghua Deng, Shuting Yan, Yan Huang, Chunyang Lei, Zhou Nie

    Fluorescent proteins (FPs) are powerful and fundamental tools in bio-analytical research, ranging from tracking biological molecules to monitoring dynamic processes in living cells. Currently, great efforts have been made on structurally engineering of FPs with new fluorescent signal-switch mechanisms via rational or semi-rational design. In this review, we summarize a variety of strategies for redesigning FPs, including circularly permuted FPs, split FPs, supercharged FPs, and unnatural amino acid-containing FPs, as well as RNA/DNA mimics of FPs. We focus on the design strategies and target-responsive mechanisms of the engineered FPs and their current applications in biosensing and bioimaging. The recent advances summarized here reveal that the great flexibility of the structure and the outstanding designability endow FPs with remarkable potential as versatile sensing elements for biochemical analysis.

    更新日期:2019-11-30
  • Chemiluminescence resonance energy transfer: from mechanisms to analytical applications
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-29
    Yongcun Yan, Xin-yan Wang, Xin Hai, Weiling Song, Caifeng Ding, Jingyu Cao, Sai Bi

    Chemiluminescence resonance energy transfer (CRET) is a non-radiative transfer of energy from chemiluminescence (CL) reagent to energy acceptor during the CL reaction. The reaction process does not require excitation from external source, so CRET-based assays have low background and high sensitivity. Recently, efficient energy transfer systems have been proposed and a variety of nanomaterials with different functions have been presented as acceptors. Among them, the "signal-on" type acceptors (e.g., fluorescence dye, quantum dots and semiconducting polymer) can increase the CL intensity effectively, while the "signal-off" type acceptors (e.g., gold nanoparticle and carbon nanomaterials) have highly quenching efficiency. The development of the acceptors has extended the applications of CRET ranging from biosensing and bioimaging to biomedicine and therapy. In this review, the CRET modes are divided according to the type of acceptors based on the different energy transfer mechanisms. Their recent advances and future prospect are summarized and discussed.

    更新日期:2019-11-29
  • Advances in oligonucleotide-based detection coupled with fluorescence resonance energy transfer
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-29
    Murali Mohana Rao Singuru, Sin-Cih Sun, Min-Chieh Chuang

    Given its unique optical properties, the Förster Resonance Energy Transfer (FRET) process that couples donor- and acceptor-fluorophores has been extensively applied to analyze a variety of substances. Moreover, the exploitation of nanomaterials has injected fresh energy into FRET development in recent decades. The present study provides an overview of state-of-the-art technology being used in oligonucleotide-based assays with output displays in relation to the FRET process. Specifically, published investigations conducted over the last decade are reviewed. Two aspects of analytes, nucleic acids and nonnucleic acids, are discussed separately; for each, we focus on six principal materials of the donor and acceptor and summarize their absorption and emission properties. The number of articles in each category is also considered to highlight trends in FRET development. Appreciable attention is paid to detailing the recognition mechanism that uses oligonucleotides and the FRET process between donor and acceptor. Sensing performance in existing studies is summarized as well. Finally, three developments in perspective are highlighted, revealing the superior and cost-effective control of FRET efficiency with respect to practical assays.

    更新日期:2019-11-29
  • Colorimetric sensor array based on gold nanoparticles: design principles and recent advances
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-28
    Jingwei Sun, Yuexiang Lu, Liuying He, Jiawei Pang, Fengyi Yang, Yueying Liu
    更新日期:2019-11-28
  • Recent Trends in Disposal and Treatment Technologies of Emerging-Pollutants- A Critical Review
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-26
    Nadeem A. Khan, Saif Ullah Khan, Sirajuddin Ahmed, Izharul Haq Farooqi, Mahmood Yousefi, Ali Akbar Mohammadi, Fazlollah Changani

    Recently pharmaceuticals are emerging as a major source of pollution for the environment. It has been well observed that the effluent discharge from hospitals has an eminent quantity of chemical waste as antibiotics, disinfectants and other treatments wastes. Pharmaceutical effluents are bioactive and their existence in the environment has been found harmful to both aquatic life and humans. In developing countries, untreated wastewaters are discharged to local water bodies by just following the local regulations. The current global challenges including high population growth rate and climate change have contributed to the widespread epidemics and emergence of diseases. Besides intractable and decaying antibiotic system, hospitals emit pathogenic waste during treatment process that can lead to situations where a total ban on hospital effluent are needed, for instance in multiple cases where discharge lead to strain on nature and quality of water. In case of pharmaceutical residues, it has been observed that only 18-32% of the pharmaceutical residues could be degraded by the secondary treatment of these seven technologies and it has been increased to 30-65% by tertiary treatment. As far as the pharmaceutical residues are concerned, it has been observed that MBR removes the residues with the efficiency of 28-100%, varying for each pharmaceutical. This paper reviews the existing treatment processes, their advantages and future perspective of this emerging area.

    更新日期:2019-11-27
  • Resonance energy transfer in electrochemiluminescent and photoelectrochemical bioanalysis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-26
    Yanxin Chu, Tingting Han, Anping Deng, Lingling Li, Jun-Jie Zhu

    Resonance energy transfer (RET) has been universally recognized as a powerful technique for probing a variety of biological processes since its discovery. Particularly, electrochemiluminescence resonance energy transfer (ECL-RET) and photoelectrochemistry resonance energy transfer (PEC-RET) have drawn increasing attention as promising analytical approaches due to their high sensitivity and low background signal. In this review, recent advances in the development of ECL-RET and PEC-RET biosensors are summarized, with emphasis on different formats of RET signaling strategies in both fields. This review also highlights the representative applications of ECL-RET and PEC-RET in various areas of importance. Finally, we give an outlook on the future development of ECL-RET and PEC-RET biosensors.

    更新日期:2019-11-27
  • Single-Molecule Fluorescence Resonance Energy Transfer and Its Biomedical Applications
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-26
    Chen-chen Li, Ying Li, Yan Zhang, Chun-yang Zhang

    Single-molecule fluorescence resonance energy transfer (smFRET) requires significant spectral overlap between the donor emission and the acceptor absorption, and its efficiency is strongly dependent on the donor-acceptor distance. Consequently, smFRET has the capability to study molecular interactions of biomolecules with sub-nanometer resolution in a homogeneous manner without the involvement of any separation steps. The smFRET has significant advantages of high signal-to-noise ratio, low sample consumption, and homogeneous assay, providing a new approach to detect low-abundant biomolecules with extremely high sensitivity. In this review, we summarize the strategies for improving smFRET efficiency and the applications of smFRET in sensitive detection of DNAs, microRNAs, enzymes, and in situ imaging of single-nucleotide mutation. Moreover, we provide new insight into the challenges and future perspectives of smFRET.

    更新日期:2019-11-27
  • Fabrication of chemiluminescence resonance energy transfer platform based on nanomaterial and its application in optical sensing, biological imaging and photodynamic therapy
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-11-25
    Jia Che, Hongdeng Qiu, Shulin Zhao

    Chemiluminescence resonance energy transfer (CRET), which involves nonradiative energy transfer from a chemiluminescent donor to a suitable acceptor molecule without the use of an external light source, has drawn considerable attention in diverse fields. This review thoroughly summarizes the latest progress (from 2014 to 2019) of CRET based on nanomaterial in many aspects including small molecule detection, macromolecule analysis, immunoassay, biological imaging, and photodynamic therapy. We emphasize on the design strategies of CRET based on nanomaterial. In addition, the challenges and future perspectives CRET based on nanomaterial are also discussed. We anticipate that this review may be a guidance for the further development of CRET based on nanomaterial in the field of life-science.

    更新日期:2019-11-26
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