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An enzyme-powered, three-dimensional lame DNA walker Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-08 Jie Fang; Changjing Yuan; Junjie Li; Junlong Li; Tingyan Yang; Yongcan Guo; Ding Wang; Jianjiang Xue; WeiLing Fu; Guoming Xie
Molecular machines constructed by three-dimensional (3-D) DNA walker have emerged as a hot topic in applications such as novel biosensors, cargo delivery platforms and intracellular imaging. Herein, we first propose a lame DNA walker that can randomly and autonomously move on microsphere-based 3-D track. The stochastic lame walker has a long leg mainly responsible for persistent movement and a short
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Transpeptidation-mediated single-particle imaging assay for sensitive and specific detection of Sortase with dark-field optical microscopy Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-15 Tongtong Tian; Jinzhi Zhao; Yuning Wang; Binxiao Li; Liang Qiao; Kun Zhang; Baohong Liu
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Fully integrated microfluidic devices for qualitative, quantitative and digital nucleic acids testing at point of care Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-31 Zedong Li; Yuemeng Bai; Minli You; Jie Hu; Chunyan Yao; Lei Cao; Feng Xu
Benefiting from emerging miniaturized and equipment-free nucleic acid testing (NAT) technologies, fully integrated NAT devices at point of care (POC) with the capability of “sample-in-answer-out” are proceeding at a break-neck speed to eliminate complex operations and reduce the risk of contamination. Like the development of polymerase chain reaction (PCR) technology (the standard technique for NAT)
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Exosome-mediated diagnosis of pancreatic cancer using lectin-conjugated nanoparticles bound to selective glycans Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-06 Yonghyun Choi; Uiseon Park; Hyung-Jun Koo; Jin-seok Park; Don Haeng Lee; Kyobum Kim; Jonghoon Choi
The unique profile of upregulated glycosylation in metastatic cancer cells may form the basis for the development of new biomarkers for the targeting and diagnosis of specific cancers. This study introduces a pancreatic cancer cell-derived exosome detection technology, which is based on the specific binding of lectins to distinctive glycan profiles on the surface of exosomes. Lectins with a high and
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Optical Bioelectronic Nose of Outstanding Sensitivity and Selectivity Toward Volatile Organic Compounds Implemented with Genetically Engineered Bacteriophage: Integrated Study of Multi-Scale Computational Prediction and Experimental Validation Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-13 JungYun Park; Jong-Min Lee; Hoje Chun; Yujin Lee; Sung Jun Hong; Hyunwook Jung; Ye-Ji Kim; Won-Geun Kim; Vasanthan Devaraj; Eun Jung Choi; Jin-Woo Oh; Byungchan Han
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Research advances for exosomal miRNAs detection in biosensing: From the massive study to the individual study Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-31 Yuan Wu; Ye Zhang; Xiaohe Zhang; Shihua Luo; Xiaohui Yan; Yurong Qiu; Lei Zheng; Ling Li
Exosomal miRNAs (exo-miRNAs) derived from cancer cell play a vital role in cancer development and can be considered as a potential biomarker for cancer diagnostic. However, the shortage of sensitive preparatory and analytical technologies limits their clinical applications. Thus, sensitive and reliable sensing of exo-miRNAs is essential in medical diagnostics. Mounting number of biosensor strategies
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Precise and rapid solvent-assisted geometric protein self-patterning with submicron spatial resolution for scalable fabrication of microelectronic biosensors Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-08 Jun'ya Tsutsumi; Anthony P.F. Turner; Wing Cheung Mak
Precise and high-resolution coupling of functional proteins with micro-transducers is critical for the manufacture of miniaturized bioelectronic devices. Moreover, electrochemistry on microelectrodes has had a major impact on electrochemical analysis and sensor technologies, since the small size of microelectrode affects the radial diffusion flux of the analyte to deliver enhanced mass transport and
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DNA pom-pom nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-12 Yan Zeng; Peng Qi; Yingwen Wang; Chao Chen; Dun Zhang
Pathogenic bacteria levels are significantly related with disease control, clinical diagnosis, and even environmental monitoring. It is becoming highly urgent to achieve ultrasensitive detection of pathogenic bacteria and efficient combat of bacterial infection. Toward this end, we have assembled a DNA Pom-Pom nanostructure (PP–N) based multifunctional platform for pathogenic bacteria determination
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Anomalous sensitivity enhancement of nano-graphitic electrochemical micro-sensors with reducing the operating voltage Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-05 Edoardo Cuniberto; Abdullah Alharbi; Zhujun Huang; Ting Wu; Roozbeh Kiani; Davood Shahrjerdi
Microscopic interactions between electrochemical sensors and biomolecules critically influence the sensitivity. Here, we report an unexpected dependence of the sensitivity on the upper potential limit (UPL) in voltammetry experiments. In particular, we find that the sensitivity of substrate-supported nano-graphitic micro-sensors in response to dopamine increases almost linearly with the inverse of
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Rapid visualized assessment of drug efficacy in live mice with a selectable marker-free autoluminescent Klebsiella pneumoniae Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-12 Xirong Tian; Yamin Gao; Shuai Wang; H.M. Adnan Hameed; Wei Yu; Cuiting Fang; Jingran Zhang; Yaoju Tan; Zhizhong Cao; Ping Guan; Jinxing Hu; Jianxiong Liu; Nanshan Zhong; Tianyu Zhang
Klebsiella pneumoniae is an opportunistic pathogen that is responsible for community acquired infections and nosocomial infections. Antibiotic-resistant K. pneumoniae and/or hypervirulent K. pneumoniae are emerging as a serious threat to public health. For the sake of alleviating and conquering current dilemma, discovery of effective new drugs against K. pneumoniae is a tough challenge. However, traditional
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Novel cytosensor for accurate detection of circulating tumor cells based on a dual-recognition strategy and BSA@Ag@Ir metallic-organic nanoclusters Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-12 Huawei Shen; Liyu Liu; Zuowei Yuan; Qian Liu; Baiying Li; Ming Zhang; Hejun Tang; Juan Zhang; Shiqiao Zhao
Herein, based on a dual-recognition strategy and BSA@Ag@Ir metallic-organic nanoclusters (BSA@Ag@Ir MONs), a highly specific and sensitive cytosensor was developed for detecting circulating tumor cells (CTCs). To amplify current signal, novel BSA@Ag@Ir MONs with outstanding catalytic activity and huge specific surface area were synthesized, and conjugated with hairpin DNA strands as signal probes.
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Electrochemical impedimetric biosensors, featuring the use of Room Temperature Ionic Liquids (RTILs): Special focus on non-faradaic sensing Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-30 Sayali Upasham; Ivneet Kaur Banga; Badrinath Jagannath; Anirban Paul; Kai-Chun Lin; Sriram Muthukumar; Shalini Prasad
Over the last decade, significant advancements have been made in the field of biosensing technology. With the rising demand for personalized healthcare and health management tools, electrochemical sensors are proving to be reliable solutions; specifically, impedimetric sensors are gaining considerable attention primarily due to their ability to perform label-free sensing. The novel approach of using
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A naked-eye readout self-powered electrochemical biosensor toward indoor formaldehyde: On-site detection and exposure risk warning Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-06 Xiaoxuan Sun; He Zhang; Liang Huang; Shuai Hao; Junfeng Zhai; Shaojun Dong
The determination of indoor formaldehyde is of great importance to protect individuals against its well-known adverse impact on health. Here, we report on a design of a naked-eye readout self-powered electrochemical biosensor (SPEB) toward gaseous formaldehyde based on the efficient catalytic activity of the formaldehyde dehydrogenase/poly (methylene green)/buckypaper bioanode and the excellent electrochromic
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Biological characteristics and biomarkers of novel SARS-CoV-2 facilitated rapid development and implementation of diagnostic tools and surveillance measures Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-04 Gajanan Sampatrao Ghodake; Surendra Krushna Shinde; Avinash Ashok Kadam; Rijuta Ganesh Saratale; Ganesh Dattatraya Saratale; Asad Syed; Abdallah M. Elgorban; Najat Marraiki; Dae-Young Kim
Existing coronavirus named as a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has speeded its spread across the globe immediately after emergence in China, Wuhan region, at the end of the year 2019. Different techniques, including genome sequencing, structural feature classification by electron microscopy, and chest imaging using computed tomography, are primarily used to diagnose and
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Miniature microbial solar cells to power wireless sensor networks Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-04 Lin Liu; Seokheun Choi
Conventional wireless sensor networks (WSNs) powered by traditional batteries or energy storage devices such as lithium-ion batteries and supercapacitors have challenges providing long-term and self-sustaining operation due to their limited energy budgets. Emerging energy harvesting technologies can achieve the longstanding vision of self-powered, long-lived sensors. In particular, miniature microbial
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PfAgo-based detection of SARS-CoV-2 Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-28 Fei Wang; Jun Yang; Ruyi He; Xiao Yu; Shuliang Chen; Yang Liu; Longyu Wang; Aitao Li; Linlin Liu; Chao Zhai; Lixin Ma
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A “hot Spot”-Enhanced paper lateral flow assay for ultrasensitive detection of traumatic brain injury biomarker S-100β in blood plasma Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-03 Xuefei Gao; Jennifer Boryczka; Peng Zheng; Sujan Kasani; Feng Yang; Elizabeth B. Engler-Chiurazzi; James W. Simpkins; Jane G. Wigginton; Nianqiang Wu
Currently colorimetric paper lateral flow strips (PLFS) encounter two major limitations, that is, low sensitivity and severe interference from complex sample matrices such as blood. These shortcomings limit their application in detection of low-concentration analytes in complex samples. To solve these problems, a PLFS has been developed by utilizing surface-enhanced Raman scattering (SERS) for sensing
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Faradaic Electrochemical Impedance Spectroscopy for Enhanced Analyte Detection in Diagnostics Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-31 Madison E. Strong; Jeffrey R. Richards; Manuel Torres; Connor M. Beck; Jeffrey T. La Belle
Electrochemical impedance spectroscopy (EIS) is a widely implementable technique that can be applied to many fields, ranging from disease detection to environmental monitoring. EIS as a biosensing tool allows detection of a broad range of target analytes in point-of-care (POC) and continuous applications. The technique is highly suitable for multimarker detection due to its ability to produce specific
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Development of a novel homogeneous immunoassay using the engineered luminescent enzyme NanoLuc for the quantification of the mycotoxin fumonisin B1 Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-30 Tawfiq Alsulami; Nidhi Nath; Rod Flemming; Hui Wang; Wenhui Zhou; Jae-Hyuk Yu
Compared to the traditional heterogeneous assays, a homogeneous immunoassay is a preferred format for its simplicity. By cloning and isolating luminescent proteins from bioluminescent organisms, bioluminescence has been widely used for various biological applications. In this study, we present the development of a homogeneous luminescence immunoassay (FNanoBiT assay) for detection of fumonisin B1 (FB1)
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DNAzyme cascade circuits in highly integrated DNA nanomachines for sensitive microRNAs imaging in living cells Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-06 Lu-Ying Duan; Jin-Wen Liu; Ru-Qin Yu; Jian-Hui Jiang
DNA molecular probes have emerged as powerful tools for fluorescence imaging of microRNAs (miRNAs) in living cells and thus elucidating functions and dynamics of miRNAs. In particular, the highly integrated DNA probes that can be able to address the robustness, sensitivity and consistency issues in a single assay system were highly desired but remained largely unsolved challenge. Herein, we reported
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Detection of Tetanus Toxoid with Fluorescent Tetanus Human IgG-AuNC–Based Immunochromatography Test Strip Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-06 Quan-Quan Zhuang; Rui-Ting Chen; Yi-Jing Zheng; Kai-Yuan Huang; Hua-Ping Peng; Zhen Lin; Xing-Hua Xia; Wei Chen; Hao-Hua Deng
Assays for detecting tetanus toxoid are of great significance to be applied in the research of the safety testing of tetanus vaccine. Currently, guinea pigs or mice are usually used to evaluate the toxicity in these assays. Herein, a facile and quick biomineralization process was carried out to generate tetanus human immunoglobulin G (Tet-IgG)-functionalized Au nanoclusters (Tet-IgG-AuNCs). The obtained
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Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-06 Arash Ahmadivand; Burak Gerislioglu; Zeinab Ramezani; Ajeet Kaushik; Pandiaraj Manickam; S. Amir Ghoreishi
Effective and efficient management of human betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 virus infection i.e., COVID-19 pandemic, required sensitive and selective sensors with short sample-to-result durations for performing desired diagnostics. In this direction, one appropriate alternative approach to detect SARS-CoV-2 virus protein at low level i.e., femtomolar (fM) is exploring
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Cryopreservation of a cell-based biosensor chip modified with elastic polymer fibers enabling ready-to-use on-site applications Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-08 Dua Özsoylu; Tuğba Isık; Mustafa M. Demir; Michael J. Schűning; Torsten Wagner
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Bacteriophage-based advanced bacterial detection: Concept, mechanisms, and applications Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-06 Wajid Hussain; Muhammad Wajid Ullah; Umer Farooq; Ayesha Aziz; Shenqi Wang
Some bacterial species are deadly disease-causing pathogens with high morbidity and mortality in humans worldwide. Key interfaces in the transmission of bacterial pathogens include food, water, dairy products, peridomestic animals, and human interplay. Early-stage detection of such bacteria is crucial in minimizing the risk of bacterial diseases and ensuring early diagnosis. Majority of the conventional
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DNAzymes as Key Components of Biosensing Systems for the Detection of Biological Targets Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-08 Ioana Cozma; Erin M. McConnell; John D. Brennan; Yingfu Li
DNAzymes are synthetic functional nucleic acids that have found widespread use in biosensing applications both for molecular recognition and signal generation. Two classes of DNAzymes have proved particularly effective for use in proof-of-concept biosensing systems, namely RNA-cleaving DNAzymes (RCDs) and peroxidase mimicking DNAzymes (PMDs). RCDs catalyze the site-specific cleavage reaction of an
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Development of glycated peptide enzyme sensor based flow injection analysis system for haemoglobin A1c monitoring using quasi-direct electron transfer type engineered fructosyl peptide oxidase Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-08 Mika Hatada; Satomi Saito; Satoshi Yonehara; Wakako Tsugawa; Ryutaro Asano; Kazunori Ikebukuro; Koji Sode
Haemoglobin A1c (hemoglobin A1c, HbA1c) is an important long-term glycemic control marker for diabetes. The aim of this study was to develop an enzyme flow injection analysis (FIA) system using engineered fructosyl peptide oxidase (FPOx) based on 2.5th generation principle for an HbA1c automated analytical system. FPOx from Phaeosphaeria nodorum (PnFPOx) was engineered by introducing a Lys residue
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Nano-FET-enabled biosensors: Materials perspective and recent advances in North America Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-30 Mohammed Sedki; Yu Shen; Ashok Mulchandani
Field-effect transistor (FET) is a very promising platform for biosensor applications due to its magnificent properties, including label-free detection, high sensitivity, fast response, real-time measurement capability, low running power, and the feasibility to miniaturize to a portable device. 1D (e.g. carbon nanotubes, Si nanowires, conductive polymer nanowires, 1D metal oxides, and others) and 2D
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Electrokinetic sandwich assay and DNA mediated charge amplification for enhanced sensitivity and specificity Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-30 Siddharth Sourabh Sahu; Christiane Stiller; Elizabeth Paz Gomero; Ábel Nagy; Amelie Eriksson Karlström; Jan Linnros; Apurba Dev
An electrical immuno-sandwich assay utilizing an electrokinetic-based streaming current method for signal transduction is proposed. The method records the changes in streaming current, first when a target molecule binds to the capture probes immobilized on the inner surface of a silica micro-capillary, and then when the detection probes interact with the bound target molecules on the surface. The difference
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Bioimpedance method for monitoring venous ulcers: Clinical proof-of-concept study Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-07 Atte Kekonen; Mikael Bergelin; Jan-Erik Eriksson; Annikki Vaalasti; Heimo Ylänen; Sami Kielosto; Jari Viik
Evaluation of wound status is typically based on means which require the removal of dressings. These procedures are often also subjective and prone to inter-observer bias. To overcome aforementioned issues a bioimpedance measurement-based method and measurement system has been developed to evaluate the state of wound healing. Purpose of this study was to assess the feasibility of the method and the
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Oxidized Titanium Carbide MXene-Enabled Photoelectrochemical Sensor for Quantifying Synergistic Interaction of Ascorbic Acid based Antioxidants System Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-07 Fangjie Han; Zhongqian Song; Jianan Xu; Mengjiao Dai; Shulin Luo; Dongxue Han; Li Niu; Zhenxin Wang
Antioxidants can protect organization from damage by scavenging of free radicals. When two kinds of antioxidants are consumed together, the total antioxidant capacity might be enhanced via synergistic interactions. Herein, we develop a simple, direct, and effective strategy to quantify the synergistic interaction between ascorbic acid (AA) and other different antioxidants by photoelectrochemical (PEC)
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Strategic design and improvement of the internal electron transfer of heme b domain-fused glucose dehydrogenase for use in direct electron transfer-type glucose sensors Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-17 Kohei Ito; Junko Okuda-Shimazaki; Katsuhiro Kojima; Kazushige Mori; Wakako Tsugawa; Ryutaro Asano; Kazunori Ikebukuro; Koji Sode
A fusion enzyme composed of an Aspergillus flavus-derived flavin adenine dinucleotide glucose dehydrogenase (AfGDH) and an electron transfer domain of Phanerochaete chrysosporium-derived cellobiose dehydrogenase (Pcyb) was previously reported to show the direct electron transfer (DET) ability to an electrode. However, its slow intramolecular electron transfer (IET) rate from the FAD to the heme, limited
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Controllable design of a nano-bio aptasensing interface based on tetrahedral framework nucleic acids in an integrated microfluidic platform Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-29 Fulin Zhu; Xiaojun Bian; Hongcai Zhang; Yanli Wen; Qian Chen; Yongliang Yan; Liang Li; Gang Liu; Juan Yan
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An assembly-regulated SNAP-tag fluorogenic probe for long-term super-resolution imaging of mitochondrial dynamics Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-01 Wenjuan Liu; Qinglong Qiao; Jiazhu Zheng; Jie Chen; Wei Zhou; Ning Xu; Jin Li; Lu Miao; Zhaochao Xu
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Novel chloramphenicol sensor based on aggregation-induced electrochemiluminescence and nanozyme amplification Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-02 Shuhuai Li; Xionghui Ma; Chaohai Pang; Mingyue Wang; Guihao Yin; Zhi Xu; Jianping Li; Jinhui Luo
Combining electrochemiluminescence (ECL) with nanozyme amplification provides unique advantages for the detection of antibiotic residues. Herein, a molecularly imprinted chloramphenicol (CAP) sensor was established based on aggregation-induced (AI)-ECL and nanozyme amplification. Covalent organic framework materials with AI-ECL groups (COF-AI-ECL) and nanozyme Co3O4 were synthesised as the signal element
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Real-time in situ monitoring of Lon and Caspase-3 for assessing the state of cardiomyocytes under hypoxic conditions via a novel Au–Se fluorescent nanoprobe Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-05 Renhui Zhan; Wenfei Guo; Xiaonan Gao; Xiaojun Liu; Kehua Xu; Bo Tang
Myocardial dysfunction caused by cardiomyocyte apoptosis under ischemic and hypoxic conditions is the pathological basis of most cardiovascular diseases. Current diagnosis of myocardial dysfunction still focuses on the symptomatic stage, usually after the occurrence of the irreversible remodelling and functional impairment. Thus, early stage identification of the apoptotic cardiomyocytes induced by
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Bovine serum albumin-stabilized silver nanoclusters with anodic electrochemiluminescence peak at 904 nm in aqueous medium and applications in spectrum-resolved multiplexing immunoassay Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-28 Lei Yu; Mengmeng Li; Qi Kang; Li Fu; Guizheng Zou; Dazhong Shen
Near-infrared electrochemiluminescence (NIR ECL) luminophores are strongly anticipated in ECL bioimaging and spectrum-resolved multiplexing assays. Herein, bovine serum albumin-stabilized Ag nanoclusters (BSA-Ag NCs) are demonstrated promising NIR ECL performance, exhibiting strong anodic ECL spectrum peak at 904 nm in aqueous medium. The ECL intensity of BSA-Ag NCs-triethanolamine system was enhanced
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Near-field sensor array with 65-GHz CMOS oscillators for rapid detection of viable Escherichia coli Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-29 Yuichi Ogawa; Shojiro Kikuchi; Yoshihisa Yamashige; Keiichiro Shiraga; Takeshi Mitsunaka
In this study, the growth of Escherichia coli was monitored using a complementary metal-oxide-semiconductor (CMOS) near-field sensor array. Each of the 1488 integrated elements, arranged in a 3 mm square, has a resonator that oscillates at 65 GHz. The effective capacitance of the resonator is altered by changes in the dielectric properties of the sensor surface, which shifts the resonance frequency
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Label-free and enzyme-free fluorescence detection of microRNA based on sulfydryl-functionalized carbon dots via target-initiated hemin/G-quadruplex-catalyzed oxidation Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-01 Jianling Chen; Ji Yan; Qiumei Feng; Xiangmin Miao; Baoting Dou; Po Wang
Carbon dots (CDs)-based biosensors have attracted considerable interest in reliable and sensitive detection of microRNA (miRNA) because of their merits of ultra-small size, excellent biosafety and tunable emission, whereas complicated labeling procedure and expensive bioenzyme associated with current strategies significantly limit their practical application. Herein, we developed a label-free and enzyme-free
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Reciprocating-flowing on-a-chip enables ultra-fast immunobinding for multiplexed rapid ELISA detection of SARS-CoV-2 antibody Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-29 Yiren Liu; Yayin Tan; Quanying Fu; Maoren Lin; Jinxu He; Suhua He; Mei Yang; Shoudeng Chen; Jianhua Zhou
The worldwide epidemic of novel coronavirus disease (COVID-19) has led to a strong demand for highly efficient immunobinding to achieve rapid and accurate on-site detection of SARS-CoV-2 antibodies. However, hour-scale time-consumption is usually required to ensure the adequacy of immunobinding on expensive large instruments in hospitals, and the common false negative or positive results often occur
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Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-31 Fengge Song; Yuanyuan Shen; Yangdao Wei; Chunrong Yang; Xiaolin Ge; Aimin Wang; Chaoyang Li; Yi Wan; Jinghong Li
The rapid identification of pathogenic microorganisms plays a crucial role in the timely diagnosis and treatment strategies during a global pandemic, especially in resource-limited area. Herein, we present a sensitive biosensor strategy depended on botulinum neurotoxin type A light chain (BoNT/A LC) activated complex assay (BACA). BoNT/A LC, the surrogate of BoNT/A which embodying the most potent biological
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Wearable sensors: At the frontier of personalised health monitoring, smart prosthetics and assistive technologies Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-30 Farnaz Khoshmanesh; Peter Thurgood; Elena Pirogova; Saeid Nahavandi; Sara Baratchi
Wearable sensors have evolved from body-worn fitness tracking devices to multifunctional, highly integrated, compact, and versatile sensors, which can be mounted onto the desired locations of our clothes or body to continuously monitor our body signals, and better interact and communicate with our surrounding environment or equipment. Here, we discuss the latest advances in textile-based and skin-like
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Aptamer-functionalized metal-organic frameworks (MOFs) for biosensing Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-30 Mengzhen Lv; Wan Zhou; Hamed Tavakoli; Cynthia Bautista; Jianfei Xia; Zonghua Wang; XiuJun Li
As a class of crystalline porous materials, metal-organic frameworks (MOFs) have attracted increasing attention. Due to the nanoscale framework structure, adjustable pore size, large specific surface area, and good chemical stability, MOFs have been applied widely in many fields such as biosensors, biomedicine, electrocatalysis, energy storage and conversions. Especially when they are combined with
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Cas12a-based electrochemiluminescence biosensor for target amplification-free DNA detection Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-01 Peng-Fei Liu; Kai-Ren Zhao; Zhi-Jun Liu; Li Wang; Shu-Ying Ye; Guo-Xi Liang
CRISPR/Cas system have drawn increasing attention in accurate and sensitive nucleic acids detection. Herein, we reported a novel Cas12a-based electrochemiluminescence biosensor for target amplification-free human papilloma virus subtype (HPV-16) DNA detection. During this detection process, Cas12a employed its two-part recognition mechanism to improve the specificity and trans-cleavage capability to
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A Critical Review: Recent Advances in “Digital” Biomolecule Detection with Single Copy Sensitivity Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-04 Haomin Liu; Yu Lei
Detection of a single biomolecule, ranging from nucleic acids, proteins, viruses to bacteria, is of paramount importance in various fields including biology, environment, food and agriculture industry, public health, and medicine. With the understanding of the biological functions of these biomolecules (or bioparticles) and their impacts on public health, environmental pollution, and food safety, advanced
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BIO bragg gratings on microfibers for label-free biosensing Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-02 Augusto Juste-Dolz; Martina Delgado-Pinar; Miquel Avella-Oliver; Estrella Fernández; Daniel Pastor; Miguel V. Andrés; Ángel Maquieira
Discovering nanoscale phenomena to sense biorecognition events introduces new perspectives to exploit nanoscience and nanotechnology for bioanalytical purposes. Here we present Bio Bragg Gratings (BBGs), a novel biosensing approach that consists of diffractive structures of protein bioreceptors patterned on the surface of optical waveguides, and tailored to transduce the magnitude of biorecognition
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Entropy-driven electrochemiluminescence ultra-sensitive detection strategy of NF-κB p50 as the regulator of cytokine storm Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-30 Kai Zhang; Zhenqiang Fan; Bo Yao; Tingting Zhang; Yuedi Ding; Sha Zhu; Minhao Xie
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Aptasensor for the detection of Methicillin resistant Staphylococcus aureus on contaminated surfaces Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-17 Muhabat Adeola Raji; Ghadeer Suaifan; Atef Shibl; Karina Weber; Dana Cialla-May; Jürgen Popp; Khaled Al-Kattan; Mohammed Zourob
There is mounting evidence that contaminated hospital environment plays a crucial role in the transmission of nosocomial pathogens such as MRSA. The institution of infection control protocols is predicated on the early laboratory detection of the pathogen from relevant samples. Processing of environmental samples for the presence of bacterial contaminants in the clinical environment is poorly standardized
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The development of an implantable deep brain stimulation device with simultaneous chronic electrophysiological recording and stimulation in humans Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-15 Abhinav Goyal; Steve Goetz; Scott Stanslaski; Yoonbae Oh; Aaron E. Rusheen; Bryan Klassen; Kai Miller; Charles D. Blaha; Kevin E. Bennet; Kendall Lee
Deep brain stimulation (DBS) is used to treat a wide array of neurologic conditions. However, traditional programming of stimulation parameters relies upon short term subjective observation of patient symptoms and undesired stimulation effects while in the clinic. To gain a more objective measure of the neuronal activity that contributes to patient symptoms and response to treatment, there is a clear
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Rational design of direct electron transfer type l-lactate dehydrogenase for the development of multiplexed biosensor Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-25 Kentaro Hiraka; Wakako Tsugawa; Ryutaro Asano; Murat A. Yokus; Kazunori Ikebukuro; Michael A. Daniele; Koji Sode
The development of wearable multiplexed biosensors has been focused on systems to measure sweat l-lactate and other metabolites, where the employment of the direct electron transfer (DET) principle is expected. In this paper, a fusion enzyme between an engineered l-lactate oxidase derived from Aerococcus viridans, AvLOx A96L/N212K mutant, which is minimized its oxidase activity and b-type cytochrome
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Facile biosensor-based system for on-site quantification of total viable counts in food and environmental swabs Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-29 Elisa Santovito; Sophia Elisseeva; Aisling Bukulin; Joseph P. Kerry; Dmitri B. Papkovsky
We describe a new biosensor platform for rapid and simple quantification of total aerobic viable counts of bacteria (TVC) in food and environmental swabs by oxygen respirometry. The system uses disposable swab vials with phosphorescent oxygen sensors integrated in the bottom part, a small block heater/incubator and a handheld sensor reader. In the testing, groups of 1–20 swabs samples were prepared
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Crispr-Powered Electrochemical Microfluidic Multiplexed Biosensor For Target Amplification-Free Mirna Diagnostics Biosens. Bioelectron. (IF 10.257) Pub Date : 2021-01-01 Richard Bruch; Midori Johnston; André Kling; Thorsten Mattmüller; Julia Baaske; Stefan Partel; Sibylle Madlener; Wilfried Weber; Gerald A. Urban; Can Dincer
Recently the use of microRNAs (miRNAs) as biomarkers for a multitude of diseases has gained substantial significance for clinical as well as point-of-care diagnostics. Amongst other challenges, however, it holds the central requirement that the concentration of a given miRNA must be evaluated within the context of other factors in order to unambiguously diagnose one specific disease. In terms of the
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Coupling of silver nanoparticle-conjugated fluorescent dyes into optical fiber modes for enhanced signal-to-noise ratio Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-24 Nhu Hoa Thi Tran; Thang Bach Phan; Than Thi Nguyen; Heongkyu Ju
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Deconvolution of WNT-induced Frizzled conformational dynamics with fluorescent biosensors Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-30 Hannes Schihada; Maria Kowalski-Jahn; Ainoleena Turku; Gunnar Schulte
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Integrating CuO/g-C3N4 p-n heterojunctioned photocathode with MoS2 QDs@Cu NWs multifunctional signal amplifier for ultrasensitive detection of AβO Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-30 Jinling Zhang; Xuechen Zhang; Yao Gao; Jianyue Yan; Wenbo Song
Superior to anodic photoelectrochemical (PEC) method, cathodic bioanalysis integrates merits of excellent anti-interference and high stability, representing a promising and competitive methodology in precise monitoring targets in complex matrices. However, serious consideration of photocathode is far behind the anodic one, developing high-performance photocathode for PEC biosensing is thus urgently
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Highly sensitive and stable fructose self-powered biosensor based on a self-charging biosupercapacitor Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-19 Paolo Bollella; Zhanna Boeva; Rose-Marie Latonen; Kenji Kano; Lo Gorton; Johan Bobacka
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An ultrasensitive impedance biosensor for Salmonella detection based on rotating high gradient magnetic separation and cascade reaction signal amplification Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-28 Fengchun Huang; Li Xue; Wuzhen Qi; Gaozhe Cai; Yuanjie Liu; Jianhan Lin
An impedance biosensor using rotary magnetic separation and cascade reaction was developed for rapid and ultrasensitive detection of Salmonella typhimurium. First, magnetic nanoparticles (MNPs) modified with anti-Salmonella monoclonal antibodies were injected into a capillary at the presence of a rotary high gradient magnetic field, which was rotated by a stepper motor. Then, a bacterial sample was
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Study of biochip integrated with microelectrodes modified by poly-dopamine-co-chitosan composite gel for separation, enrichment and detection of microbes in the aerosol Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-24 Xi Su; Rui Ren; Yin Wu; Shifang Li; Chuang Ge; Lulu Liu; Yi Xu
As the urgent need for rapid detection of airborne microbes in a specific environment, a biochip which was integrated with the functions of enrichment and detection was designed and developed. It was composed of cover plate, copper microelectrodes modified with poly-dopamine-co-chitosan (PDA-co-CS) composite gel, sealing washer and substrate containing copper sheet electrode. The microbes were enriched
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Anti-fouling poly adenine coating combined with highly specific CD20 epitope mimetic peptide for rituximab detection in clinical patients’ plasma Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-10-03 Shengfeng Huang; Rentao Tang; Tingting Zhang; Jing Zhao; Zhengjin Jiang; Qiqin Wang
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Solid-phase microextraction integrated nanobiosensors for the serial detection of cytoplasmic dopamine in a single living cell Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-28 Yaran Chang; Yongjia Chen; Yunlong Shao; Boye Li; Yuanyuan Wu; Wenmei Zhang; Yingyan Zhou; Zhihui Yu; Liping Lu; Xiayan Wang; Guangsheng Guo
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Induced bioresistance via BNP detection for machine learning-based risk assessment Biosens. Bioelectron. (IF 10.257) Pub Date : 2020-12-16 Seth So; Aya Khalaf; Xinruo Yi; Connor Herring; Yingze Zhang; Marc A. Simon; Murat Akcakaya; SeungHee Lee; Minhee Yun
Machine Learning (ML) is a powerful tool for big data analysis that shows substantial potential in the field of healthcare. Individual patient data can be inundative, but its value can be extracted by ML's predictive power and ability to find trends. A great area of interest is early diagnosis and disease management strategies for cardiovascular disease (CVD), the leading cause of death in the world
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