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Renin imprinted Poly(methyldopa) for biomarker detection and disease therapy Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-14 Xiaofeng Sun, Tianqing Hu, Yuexia Bai, Tianyu Cao, Shuai Wang, Wei Hu, Huan Yang, Xiliang Luo, Min Cui
Conventional molecularly imprinted polymers (MIPs) perform their functions principally depended on their three dimensional (3D) imprinted cavities (recognition sites) of templates. Here, retaining the function of recognition sites resulted from the imprinting of template molecules, the role of functional monomers is explored and expanded. Briefly, a class of dual-functional renin imprinted poly(methyldopa)
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Urinary dengue NS1 detection on Au-decorated ZnO nanowire platform Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-13 Kannika Sitthisuwannakul, Ratchanon Sukthai, Zetao Zhu, Kazuki Nagashima, Kunanon Chattrairat, Supranee Phanthanawiboon, Annop Klamchuen, Sakon Rahong, Yoshinobu Baba, Takao Yasui
Biodetection for non-invasive diagnostics of fluids, especially urine, remains a challenge to scientists due to low target concentrations. And biological complexes of the detection target may contain contaminants that also interfere with any assay. Dengue non-structural 1 protein (Dengue NS1) is an important biomarker for dengue hemorrhagic fever and dengue shock syndrome. Here, we developed an Au-decorated
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Label-free plasmonic spectral profiling of serum DNA Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-13 Peng Zheng, Piyush Raj, Le Liang, Lintong Wu, Santosh Kumar Paidi, Jeong Hee Kim, Ishan Barman
Genetic and epigenetic modifications are linked to the activation of oncogenes and inactivation of tumor suppressor genes. Likewise, the associated molecular alternations can best inform precision medicine for personalized tumor treatment. Therefore, performing characterization of genetic and epigenetic alternations at the molecular level represents a crucial step in early diagnosis and/or therapeutics
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Insulin biotrapping using plasmofluidic optical fiber chips: A benchmark Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-12 Médéric Loyez, Hadrien Fasseaux, Maxime Lobry, Ruddy Wattiez, Christophe Caucheteur
Plasmonic optical fiber-based biosensors are currently in their early stages of development as practical and integrated devices, gradually making their way towards the market. While the majority of these biosensors operate using white light and multimode optical fibers (OFs), our approach centers on single-mode OFs coupled with tilted fiber Bragg gratings (TFBGs) in the near-infrared wavelength range
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Aggregation-induced emission of TTCPy-3: A novel approach for eradicating Nocardia seriolae infections in aquatic fishes Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-11 Zhigang Liu, Junling Zhang, Xiaona Ma, Miao Wang, Lijin Jiang, Meiyan Zhang, Maixin Lu, Ouqin Chang, Jianmeng Cao, Xiaoli Ke, Mengmeng Yi
Aquatic fishes are threatened by the strong pathogenic bacterium , which challenges the current prevention and treatment approaches. This study introduces luminogens with aggregation-induced emission (AIE) as an innovative and non-antibiotic therapy for . . Specifically, the AIE photosensitizer, TTCPy-3 is employed against . . We evaluated the antibacterial activity of TTCPy-3 and investigated the
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A generalizable sensing platform based on molecularly imprinted polymer-aptamer double recognition and nanoenzyme assisted photoelectrochemical-colorimetric dual-mode detection Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-11 Ying-Zhuo Shen, Wen Zheng Xie, Zheng Wang, Kang Ping Ning, Zheng Ping Ji, Hong Bo Li, Xiao-Ya Hu, Cheng Ma, Xu Qin
Developing highly sensitive and selective methods that incorporate specific recognition elements is crucial for detecting small molecules because of the limited availability of small molecule antibodies and the challenges in obtaining sensitive signals. In this study, a generalizable photoelectrochemical-colorimetric dual-mode sensing platform was constructed based on the synergistic effects of a molecularly
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Fluorescent responsive membrane based on terbium coordination polymer and carbon dots with AIE effect for rapid and visual detection of fluoroquinolone Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-11 Yunyun Hu, Xi Chen, Kai Wang, Chuang Jiang, Wenya Liu, Siyu Zhang, Mingming Zheng, Yibin Zhou, Yaqing Xiao, Yingnan Liu
In this study, based on aggregation-induced emission (AIE) effect and antenna effect, a novel portable fluorescent responsive membrane was constructed with red carbon dots (R-CDs) as reference signal and terbium coordination polymer (Tb-AMP CPs) as response signal for visual, instrument-free, and sensitive detection of fluoroquinolones (FQs). Specifically, the fluorescent responsive membrane (R-T membrane)
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Integrating target-responsive microfluidic-based biosensing chip with smartphone for simultaneous quantification of multiple fluoroquinolones Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-11 Mingming Zhang, Xue Wang, Shuangshuang Liu, Tahreem Riaz, Quansheng Chen, Qin Ouyang
The presence of fluoroquinolone (FQs) antibiotic residues in the food and environment has become a significant concern for human health and ecosystems. In this study, the background-free properties of upconversion nanoparticles (UCNPs), the high specificity of the target aptamer (Apt), and the high quenching properties of graphene oxide (GO) were integrated into a microfluidic-based fluorescence biosensing
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A point-of-care electrochemical biosensor for the rapid and sensitive detection of biomarkers in murine models with LPS-induced sepsis Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-10 Neil Adrian P. Ondevilla, Peng-Wen Liu, Wan-Ting Huang, Tzu-Ping Weng, Nan-Yao Lee, Syu-Cing Ma, Jian-Jang Huang, Tak-Wah Wong, Hsien-Chang Chang
Sepsis is a life-threatening condition, which is irreversible if diagnosis and intervention are delayed. The response of the immune cells towards an infection triggers widespread inflammation through the production of cytokines, which may result in multiple organ dysfunction and eventual death. Conventional detection techniques fail to provide a rapid diagnosis because of their limited sensitivity
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Highly catalytic Prussian blue analogues and their application on the three-dimensional origami paper-based sweat sensors Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-09 Wei-Ting Chen, Chi-Fong Yan, Cheng-Ju Yu, Ying-Chih Liao, Chien-Fu Chen
Prussian blue analogues (PBAs) are promising materials due to their rich active sites and straightforward synthesis. However, their limited conductivity and electron transfer inefficiency hinder practical applications. This study utilizes a simple one-pot synthesis approach to produce a tungsten-disulfide (WS) and iron-cobalt Prussian blue analogue composite (WS-PBA), enhancing conductivity and electron
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A handheld isothermal fluorescence detector for duplex visualization of aquatic pathogens via enhanced one-pot LAMP-PfAgo assay Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-08 Feibiao Pang, Tao Zhang, Fengyi Dai, Kaizheng Wang, Tianjiao Jiao, Zuoying Zhang, Liyi Zhang, Mingli Liu, Peng Hu, Jinzhao Song
The expansion of large-scale aquaculture has exacerbated the challenge of aquatic diseases, resulting in substantial economic losses annually. Currently, traditional laboratory-based diagnostic methods are time-consuming and costly, hindering on-site testing for individual farmers. We address this issue by developing a state-of-the-art handheld isothermal nucleic acid amplification device (WeD-1) capable
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Multi-scale bioimpedance flexible sensing with causal hierarchical machine learning for fish vitality evaluation under adversity stress Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-08 Luwei Zhang, Chuiyu Kong, You Li, Yanfu He, Xiangyun Guo, Dongjie Shi, Xiaoshuan Zhang, Jinyou Hu
It is expected that waterless low-temperature stressful environments will induce stress responses in fish and affect their vitality. In this study, we developed a laser-activated, stretchable, highly conductive liquid metal (LM) based flexible sensor system for fish multi-scale bioimpedance detection. It has excellent conformability, electrical conductivity, bending and cyclic tensile stability. Meanwhile
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Rapid and accurate SERS assay of disease-related nucleic acids based on isothermal cascade signal amplifications of CRISPR/Cas13a system and catalytic hairpin assembly Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-06 Jingjing Zhang, Zhilong Chen, Huiming Lv, Jing Liang, Chenlong Yan, Chunyuan Song, Lianhui Wang
Developing rapid, accurate and convenient nucleic acid diagnostic techniques is essential for the prevention and control of contagious diseases that are prone to gene mutations and may have homologous sequences, especially emerging infectious diseases such as the SARS-CoV-2 pandemic. Herein, a one-pot SERS assay integrating isothermal cascade signal amplification strategy (i.e., CRISPR/Cas13a system
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Development of highly sensitive dual-enhanced fluorescence quenching immunochromatographic test strips based on Pt nanoprobes Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-06 Jiashuo Xu, Shuang Zhao, Qiuting Zhang, Xu Huang, Kang Du, Jinzhi Wang, Jiaxun Wang, Cheng Chen, Bingbo Zhang, Jin Chang, Xiaoqun Gong
The fluorescence-quenching method is crucial in vitro analysis, particularly for immunochromatographic test strips (ICTs) using noble metal nanoparticles as probes. However, ICTs still fall short in meeting the requirements for the detection of traces biomarkers due to the noble metal nanoparticles can only quench fluorescence of the dyes within a confined distance. Interestingly, noble metal nanoparticles
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One-step construction of multiplexed enzymatic biosensors using light-addressable electrochemistry on a single silicon photoelectrode Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-06 Qiaoyu Yang, Jiaming Liao, Luyao Feng, Sen Wang, Zhibin Zhao, Jian Wang, Yazhong Bu, Jian Zhuang, De-Wen Zhang
The multiplexed detection of metabolites in parallel within a single biosensor plate is sufficiently valuable but also challenging. Herein, we combine the inherent light addressability of silicon with the high selectivity of enzymes, for the construction of multiplexed photoelectrochemical enzymatic biosensors. To conduct a stable electrochemistry and reagentless biosensing on silicon, a new strategy
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Polymerized carbon dots with high electrochemiluminescence efficiency and long wavelength ECL emission for ultrasensitive detection of MicroRNA-222 Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-05 Shuang Xiao, Yu-Ting Yang, Yi-Fei Chen, Jia-Li Liu, Ya-Qin Chai, Ruo Yuan
Herein, a new electrochemiluminescence (ECL) biosensor was constructed with highly efficient polymerized carbon dots (PCDs) as ECL emitter and the improved localized catalytic hairpin assembly (L-CHA) as signal amplifier for ultrasensitive detection of microRNA-222 (miRNA-222). Impressively, compared to the traditional carbon dots with inefficient blue region ECL emission, PCDs with N, O co-dope and
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Self-powered optical fiber biosensor integrated with enzymes for non-invasive glucose sensing Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-05 Xingyue Wen, Xinghua Yang, Zhongxuan Ge, Hongyu Ma, Rui Wang, Fengjun Tian, Pingping Teng, Shuai Gao, Kang Li, Bo Zhang, Sivagunalan Sivanathan
To alleviate the discomfort associated with frequent blood glucose detection in diabetic patients, a novel non-invasive tear glucose biosensor has been developed. This involved the design and preparation of a photoelectrochemical probe based on an optical fiber and biological enzymes. One end of the optical fiber connects to a light source, acting as an energy source and imparting, self-powered capability
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Development of a point-of-care colorimetric metabolomic sensor platform Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-04 Songtian Bai, Pablo Gonzalez-Vasquez, Claudia Torres-Calzada, Scott MacKay, James Cook, Yeganeh Khaniani, Gareth Davies, Upasana Singh, Prashanthi Kovur, Jie Chen, David S. Wishart
Metabolomics is the large-scale study of small molecule metabolites within a biological system. It has applications in measuring dietary intake, predicting heart disease risk, and diagnosing cancer. Metabolites are often measured using high-end analytical tools such as mass spectrometers or large spectrophotometers. However, due to their size, cost, and need for skilled operators, using such equipment
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Electrochemical biosensor using direct electron transfer and an antibody–aptamer hybrid sandwich for target detection in complex biological samples Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-03 Gyeongho Kim, Haesik Yang
Direct electron transfer (DET) between an electrode and redox labels is feasible in electrochemical biosensors using small aptamer–aptamer sandwiches; however, its application is limited in biosensors that rely on larger antibody–antibody sandwiches. The development of sandwich-type biosensors utilizing DET is challenged by the scarcity of aptamer–aptamer sandwich pairs with high affinity in complex
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Ultrasimple size encoded microfluidic chip for rapid simultaneous multiplex detection of DNA sequences Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-01 Man Tang, Kuan-Jie Zhu, Wei Sun, Xinyue Yuan, Zhipeng Wang, Ruyi Zhang, Zhao Ai, Kan Liu
Simultaneous multiplexed analysis can provide comprehensive information for disease diagnosis. However, the current multiplex methods rely on sophisticated barcode technology, which hinders its wider application. In this study, an ultrasimple size encoding method is proposed for multiplex detection using a wedge-shaped microfluidic chip. Driving by negative pressure, microparticles are naturally arranged
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Electrochemical DNA-based sensors for measuring cell-generated forces Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-01 Mahmoud Amouzadeh Tabrizi, Priyanka Bhattacharyya, Ru Zheng, Mingxu You
Mechanical forces play an important role in cellular communication and signaling. We developed in this study novel electrochemical DNA-based force sensors for measuring cell-generated adhesion forces. Two types of DNA probes, i.e., tension gauge tether and DNA hairpin, were constructed on the surface of a smartphone-based electrochemical device to detect piconewton-scale cellular forces at tunable
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SWCNTs/PEDOT:PSS nanocomposites-modified microelectrode arrays for revealing locking relations between burst and local field potential in cultured cortical networks Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-03-01 Yaoyao Liu, Shihong Xu, Yu Deng, Jinping Luo, Kui Zhang, Yan Yang, Longze Sha, Ruilin Hu, Zhaojie Xu, Erwei Yin, Qi Xu, Yirong Wu, Xinxia Cai
Burst and local field potential (LFP) are fundamental components of brain activity, representing fast and slow rhythms, respectively. Understanding the intricate relationship between burst and LFP is crucial for deciphering the underlying mechanisms of brain dynamics. In this study, we fabricated high-performance microelectrode arrays (MEAs) using the SWCNTs/PEDOT:PSS nanocomposites, which exhibited
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A novel fluorescent aptasensor for sensitive and selective detection of environmental toxins fumonisin B1 based on enzyme-assisted dual recycling amplification and 2D δ-FeOOH-NH2 nanosheets Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-29 Xiang Li, Yunxiao Ma, Mengyuan He, Bing Tan, Gongke Wang, Guifen Zhu
Fumonisin (FB) is a pervasive hazardous substance in the environment, presenting significant threats to human health and ecological systems. Thus, the selective and sensitive detection of fumonisin B1 (FB1) is crucial due to its high toxicity and wide distribution in corn, oats, and related products. In this work, we developed a novel and versatile fluorescent aptasensor by combining enzyme-assisted
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A novel ultrasensitive RNase H assay based on phosphorothioated-terminal hairpin formation and self-priming extension reaction Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-29 Junhyeok Yoon, Jinhwan Lee, Jaemin Kim, Sang Mo Lee, Soohyun Kim, Hyun Gyu Park
We herein present a novel ultrasensitive RNase H assay based on phosphorothioated-terminal hairpin formation and self-priming extension (PS-THSP) reaction. The detection probe employed as a key component in this technique serves as a substrate for RNase H and triggers the PS-THSP reaction upon the RNase H-mediated degradation of the probe. As a consequence, a large number of long concatemeric amplification
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Making commercial bracelet smarter with a biochemical button module Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-29 Junhao Chen, Xunshun Tao, Xiaohu Xu, Linan Sun, Ruquan Huang, Azadeh Nilghaz, Junfei Tian
Despite the rapid development of mobile health based on wearable devices in recent years, lack of access to biochemical detection remains a vital challenge for most commercial wearable devices, which hinders the provision of effective electronic health records (EHRs) for disease control strategies, and further constraining the development of personalized precision medicine. Herein, we propose a strategy
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A triple read-out visible biosensing platform based on multifunctional nanozyme and bipolar electrode for multi-mode detection and imaging of CEA Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-28 Shumin Wang, Xinyue Li, Xinli Wang, Xiaodi Wu, Degang Jiang, Hong Zhou, Shunxiang Gao, Jing Liu
In this paper, a proposal of closed bipolar electrode (BPE) and nanozyme based multi-mode biosensing platform is first presented. As a novel integrated chip, multi-mode-BPE (MMBPE) combines enzyme-linked immunoassay (ELISA), electrochemiluminescence (ECL), ECL imaging and light emitting diode (LED) imaging, enabling highly sensitive triple read-out visible detection of cancer embryonic antigen (CEA)
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Highly selective nanozyme-based glucose sensing platform via construction of artificial recognition sites on gold nanospheres Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-28 Jinping Yu, Tao Chen, Xueyun Wen, Hao Shi, Lupeng Wang, Yuanhong Xu
Nanozymes have been regarded as the ideal alternatives to natural enzymes in bioassays due to their good stability and low cost. However, their applications in sensing usually suffer from poor selectivity. For example, Au-based nanozymes, as a kind of classical glucose oxidase mimic enzyme, could catalyze diverse monosaccharides. Therefore, it is of great necessity and urgency to endow the Au-based
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In situ self-referenced intracellular two-electrode system for enhanced accuracy in single-cell analysis Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-28 Qingda Xu, Ye Xi, Longchun Wang, Mengfei Xu, Tao Ruan, Zhiyuan Du, Chunpeng Jiang, Jiawei Cao, Xiantao Zhu, Xiaolin Wang, Bin Yang, Jingquan Liu
Since the emergence of single-cell electroanalysis, the two-electrode system has become the predominant electrochemical system for real-time behavioral analysis of single-cell and multicellular populations. However, due to the transmembrane placement of the two electrodes, cellular activities can be interrupted by the transmembrane potentials, and the test results are susceptible to influences from
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Dual “on-off” signal conversion strategy based on surface plasmon coupling and resonance energy transfer for visual electrochemiluminescence ratiometric analysis of MiRNA-141 Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-27 Yin-Zhu Wang, Ling-Ling Wang, Yan Liu, Yu-Qi Zhang, Meng-Li Li, Chuan-Xiang Chen, Jia-Wan Zhu, Fu Yang, Yong-Hong Hu
An electrochemiluminescence (ECL) biosensor with a pair of new ECL emitters and a novel sensing mechanism was designed for the high-sensitivity detection of microRNA-141 (miRNA-141). Sulfur-doped boron nitrogen quantum dots (S-BN QDs) were initially employed to modify the cathode of the bipolar electrode (BPE), while the anode reservoir was [Ir(dfppy)(bpy)]PF/TPrA system. The next step involved attaching
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Ultra-conformal epidermal antenna for multifunctional motion artifact-free sensing and point-of-care monitoring Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-24 Zengxiang Wang, Xia Xiao, Wenqi Wu, Xiaofeng Zhang, Yanwei Pang
Accurate acquisition of physiological and physical information from human tissue is essential for health monitoring, disease prevention and treatment. The existing antennas with traditional rigid or flexible substrates are susceptible to motion artifacts in wearable applications due to the miniaturization limitation and lack of proper adhesion and conformal interfaces with the skin. Recent advances
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Cascade reaction triggered colorimetric array for identification of organophosphorus pesticides congeners Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-24 Zikang Chen, Ming Li, Weiwei Chen, Jingyi Zhou, Xungang Gu, Caiping Ding, Youju Huang
A modern agriculture uses alternative pest control methods to boost productivity, leading to an accumulation of organophosphorus (OPPs) congeners. This necessitates an intuitive and quick way to identify OPPs congeners. A colorimetric sensor for detecting OPPs congeners using a double-enzyme cascade reaction has been successfully designed and constructed in this study. The OPPs regulate the color changes
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Fibroblast activation protein-sensitive polymeric nanobeacon for early diagnosis of renal fibrosis Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-24 Ni Zeng, Xiuhong Guan, Xiaowen Liu, Huiqiu Shi, Nan Li, Ruimeng Yang, Yibo Zhou
Early diagnosis and treatment of renal fibrosis (RF) significantly affect the clinical outcomes of chronic kidney diseases (CKDs). As the typical fibrotic ailment, RF is characterized by remodeling of the extracellular matrix, and the activation of fibroblast activation protein (FAP) plays a crucial role in the mediation of extracellular matrix protein degradation. Therefore, FAP can serve as a biomarker
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Label-free fluorescent sensor for sensitive detection of ctDNA based on water stabilized CsPbBr3 nanosheet Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-23 Xiao Yang, Liangyi Zhao, Siyi Yang, Miao Tang, Huanbao Fa, Danqun Huo, Changjun Hou, Mei Yang
The detection of circulating tumor DNA (ctDNA), as a practical liquid biopsy technique, was of great significance for the study of cancer diagnosis and prognosis. However, reported methods for detection ctDNA still have some limitations, such as tedious process and high cost. In this study, CsPbBr nanosheet (CsPbBr NS) with high water stability was prepared by etching, and its fluorescence intensity
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Sea hedgehog-inspired surface-enhanced Raman scattering biosensor probe for ultrasensitive determination of Staphylococcus aureus in food supplements Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-23 Yi Tao, Qing Liu, Ningtao Cheng
contamination in food supplements poses substantial challenges to public health and large-scale production but the sensitive detection in a timely manner remains a bottleneck. Drawing inspiration from the sea hedgehog, gold nanostars (AuNSs) were leveraged to design an ultrasensitive surface-enhanced Raman scattering (SERS) biosensor for the determination of in food supplements. Besides the surface
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A skin-interfaced, miniaturized platform for triggered induction, capture and colorimetric multicomponent analysis of microliter volumes of sweat Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-23 Joohee Kim, Seyong Oh, Da Som Yang, Larissa Rugg, Radhika Mathur, Sung Soo Kwak, Seonggwang Yoo, Shupeng Li, Evangelos E. Kanatzidis, Geumbee Lee, Hong-Joon Yoon, Yonggang Huang, Roozbeh Ghaffari, Susanna A. McColley, John A. Rogers
Eccrine sweat can serve as a source of biomarkers for assessing physiological health and nutritional balance, for tracking loss of essential species from the body and for evaluating exposure to hazardous substances. The growing interest in this relatively underexplored class of biofluid arises in part from its non-invasive ability for capture and analysis. The simplest devices, and the only ones that
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Effective enrichment of trace exosomes for the label-free SERS detection via low-cost thermophoretic profiling Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-23 Yu Guo, Ruiyuan Zhang, Hongjun You, Jixiang Fang
Exosome-based liquid biopsies possess great potential in monitoring cancer development However, current exosome detection biosensors require large exosome volumes, showing the weak detection sensitivity. Besides, these methods pay little attention to in situ analysis of exosomes, hence limiting the provision of more accurate clinically-relevant information. Herein, we develop an innovative label-free
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An automatic glucose monitoring system based on periplasmic binding proteins for online bioprocess monitoring Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-23 Hasibul Hasan, Vikash Kumar, Xudong Ge, Chad Sundberg, Christopher Slaughter, Govind Rao
Glucose is one of the most vital nutrients in all living organisms, so its monitoring is critical in healthcare and bioprocessing. Enzymatic sensors are more popular as a technology solution to meet the requirement. However, periplasmic binding proteins have been investigated extensively for their high sensitivity, enabling microdialysis sampling to replace existing complex and expensive glucose monitoring
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A highly integrated digital PCR system with on-chip heating for accurate DNA quantitative analysis Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-23 Kang Peng, Zhihong Wu, Zhongxin Feng, Ruijun Deng, Xiangguo Ma, Beiyuan Fan, Haonan Liu, Zhuzhu Tang, Zijian Zhao, Yanzhao Li
Digital polymerase chain reaction (dPCR) is extensively used for highly sensitive disease diagnosis due to its single-molecule detection ability. However, current dPCR systems require intricate DNA sample distribution, rely on cumbersome external heaters, and exhibit sluggish thermal cycling, hampering efficiency and speed of the dPCR process. Herein, we presented the development of a microwell array
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Two Birds with one Stone: Dual-mode immunoassay constructed using a novel emitter ethylene glycol-induced perylene diimide and a multifunctional ANS probe Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-22 Wenjing Lai, Shijie Yan, Mingzhe Jiang, Zhina Li, Min Wang, Chulei Zhao, Chaoyun Ma, Zhong Wei, Chenglin Hong
Perylene diimide (PDI) is a readily reducible electron-deficient dye that exhibits strong photoluminescent properties, providing new opportunities for synthesizing novel electrochemiluminescence (ECL) emitters. In this study, ethylene glycol (EG) was used to induce the self-assembly of PDI supramolecules for the preparation of ultrathin EG-PDI nanosheets characterized by low crystallinity and weak
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A novel electrochemical strategy to detect hydrogen peroxide by utilizing peroxidase-mimicking activity of cerium oxide/graphene oxide nanocomposites Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-22 Sunghyeon Kim, Shin Chan Kang, Sang Mo Lee, Jinhwan Lee, Youngran Cho, Yeonjin Shim, Hyun Gyu Park
We herein describe a novel electrochemical strategy to detect hydrogen peroxide (HO) by utilizing the peroxidase-mimicking activity of cerium oxide nanoparticles (CeO NP) and reduced graphene oxide (rGO). Particularly, CeO NP/rGO nanocomposites were deposited on the commercial electrode by a very convenient and direct electrochemical reduction of graphene oxide. Due to the peroxidase-mimicking activity
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Enhanced selective discrimination of point-mutated viral RNA through false amplification regulatory direct insertion in rolling circle amplification Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-21 Hakbeom Lee, Juneseok You, Hansol Lee, Woojoo Kim, Kuewhan Jang, Jinsung Park, Sungsoo Na
Coronaviruses are single-stranded RNA viruses with high mutation rates. Although a diagnostic method for coronaviruses has been developed, variants appear rapidly. Low test accuracy owing to single-point mutations is one of the main factors in the failure to prevent the early spread of coronavirus infection. Although reverse transcription-quantitative polymerase chain reaction can detect coronavirus
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Dual-responsive 3D DNA nanomachines cascaded hybridization chain reactions for novel self-powered flexible microRNA-detecting platform Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-20 Futing Wang, Chunxiao Zhang, Suping Deng, Yifei Jiang, Penghui Zhang, Hongfen Yang, Li Xiang, Yifan Lyu, Ren Cai, Weihong Tan
The microRNA-21 is closely related to chromatin remodeling and epigenetic regulation. In this work, an efficient double-response 3D DNA nanomachine (DRDN) was assembled by co-immobilizing two different lengths of hairpin DNA on the surface of gold nanoparticles (AuNPs) to capture microRNA-21 (miRNA-21), recycle miRNA-21, and trigger hybridization chain reactions (HCR). This work reports the fabrication
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Multifunctional self-priming hairpin probe-based isothermal nucleic acid amplification and its applications for COVID-19 diagnosis Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-20 Hansol Kim, Seoyoung Lee, Yong Ju, Hyoyong Kim, Hyowon Jang, Yeonkyung Park, Sang Mo Lee, Dongeun Yong, Taejoon Kang, Hyun Gyu Park
We herein present a multifunctional self-priming hairpin probe-based isothermal amplification, termed MSH, enabling one-pot detection of target nucleic acids. The sophisticatedly designed multifunctional self-priming hairpin (MSH) probe recognizes the target and rearranges to prime itself, triggering the amplification reaction powered by the continuously repeated extension, nicking, and target recycling
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Label-free single-cell analysis in microdroplets using a light-scattering-based optofluidic chip Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-20 Li Liang, Minhui Liang, Zewen Zuo, Ye Ai
Droplet-based single-cell analysis is a very powerful tool for studying phenotypic and genomic heterogeneity at single-cell resolution for a variety of biological problems. In conventional two-phase droplet microfluidics, due to the mismatch in optical properties between oil and aqueous phases, light scattering mainly happens at the oil/water interface that disables light-scattering-based cell analysis
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Capacitive immunosensing at gold nanoparticle-decorated reduced graphene oxide electrodes fabricated by one-step laser nanostructuration Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-20 Danilo Echeverri, Enric Calucho, Jose Marrugo-Ramírez, Ruslán Álvarez-Diduk, Jahir Orozco, Arben Merkoçi
Nanostructured electrochemical biosensors have ushered in a new era of diagnostic precision, offering enhanced sensitivity and specificity for clinical biomarker detection. Among them, capacitive biosensing enables ultrasensitive label-free detection of multiple molecular targets. However, the complexity and cost associated with conventional fabrication methods of nanostructured platforms hinder the
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Microfabrication of engineered Lactococcus lactis biocarriers with genetically programmed immunorecognition probes for sensitive lateral flow immunoassay of antibiotic in milk and lake water Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-19 Fuyuan Zhang, Jiajie Chen, Fangkun Zhao, Minxuan Liu, Kaige Peng, Yuanhao Pu, Yaxin Sang, Shuo Wang, Xianghong Wang
Micro/nanomaterials display considerable potential for increasing the sensitivity of lateral flow immunoassay (LFIA) by acting as 3D carriers for both antibodies and signals. The key to achieving high detection sensitivity depends on the probe's orientation on the material surface and its multivalent biomolecular interactions with targets. Here, we engineer as the bacterial microcarrier (BMC) for a
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A personal glucose meter-utilized strategy for portable and label-free detection of hydrogen peroxide Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-19 Sang Mo Lee, Hyoyong Kim, Junhyeok Yoon, Yong Ju, Hyun Gyu Park
Rapid and precise detection of hydrogen peroxide (HO) holds great significance since it is linked to numerous physiological and inorganic catalytic processes. We herein developed a label-free and washing-free strategy to detect HO by employing a hand-held personal glucose meter (PGM) as a signal readout device. By focusing on the fact that the reduced redox mediator ([Fe(CN)]) itself is responsible
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Amplification-free quantitative detection of genomic DNA using lateral flow strips for milk authentication Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-18 Nan Wang, Juan Zhang, Bin Xiao, Xiaoyun Sun, Jiaci Chen, Fengchun Huang, Ailiang Chen
With the globalization and complexity of the food supply chain, the market is becoming increasingly competitive and food fraudulent activities are intensifying. The current state of food detection faced two primary challenges. Firstly, existing testing methods were predominantly laboratory-based, requiring complex procedures and precision instruments. Secondly, there was a lack of accurate and efficient
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Fluorophore and nanozyme-functionalized DNA walking: A dual-mode DNA logic biocomputing platform for microRNA sensing in clinical samples Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-17 Jingyuan Yu, Quanyi Liu, Lijuan Qi, Qi Fang, Xudong Shang, Xiaojun Zhang, Yan Du
Inspired by the programmability and modifiability of nucleic acids, point-of-care (POC) diagnostics for nucleic acid target detection is evolving to become more diversified and intelligent. In this study, we introduce a fluorescent and photothermal dual-mode logic biosensing platform that integrates catalytic hairpin assembly (CHA), toehold-mediated stand displacement reaction (SDR) and a DNA walking
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Wireless and battery-free wearable biosensing of riboflavin in sweat for precision nutrition Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-17 Zhenghan Shi, Peixue Deng, Li-ang Zhou, Meng Jin, Feiyue Fang, Tao Chen, Guang Liu, Hao Wen, Zijian An, Hao Liang, Yanli Lu, Jun Liu, Qingjun Liu
Nutrition assessment is crucial for dietary guidance and prevention of malnutrition. Recent endeavors in wearable biochemical sensors have enabled real-time, in situ analysis of nutrients in sweat. However, the monitoring of riboflavin, an indispensable vitamin B involved in energy metabolism, remains challenging due to its trace level and variations in the sweat matrix. Herein, we report a wireless
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A trivalent aptasensor by using DNA tetrahedron as scaffold for label-free determination of antibiotics Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-16 Tai Ye, Yimin Xu, Haohao Chen, Min Yuan, Hui Cao, Liling Hao, Xiuxiu Wu, Fengqin Yin, Fei Xu
Owing to advantage in high sensitivity and fast response, aptamer based electrochemical biosensors have attracted much more attention. However, inappropriate interfacial engineering strategy leads to poor recognition performance, which ascribe to the following factors of immobilized oligonucleotide strand including steric hindrance, interchain entanglement, and unfavorable conformation. In this work
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MoO3/MIL-125-NH2 with boosted peroxidase-like activity for electrochemical staphylococcus aureus sensing via specific recognition of bacteriophages Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-16 Yajun Gao, Suhui Xu, Ge Guo, Yajie Li, Wenyuan Zhou, Huaxiang Li, Zhenquan Yang
Herein, novel nanozyme mimics MoO/MIL-125-NH were reported and conjugated with bacteriophages as a new electrochemical probe for high sensitivity and specific electrochemical detection of . The excellent peroxidase-like activity of MoO/MIL-125-NH composites was attributed to the integration of MIL-125-NH with MoO, which can boost the generation of superoxide radicals (O• 2) and thus promote the oxidation
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A versatile upconversion-based multimode lateral flow platform for rapid and ultrasensitive detection of microRNA towards health monitoring Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-16 Cong Chen, Songtao Hu, Lulu Tian, Manlin Qi, Zhiyong Chang, Liang Li, Lin Wang, Biao Dong
MicroRNAs are small single-stranded RNA molecules associated with gene expression and immune response, suggesting their potential as biomarkers for health monitoring. Herein, we designed a novel upconversion-based multimode lateral flow assay (LFA) system to detect microRNAs in body fluids by simultaneously producing three unique signals within a detection strip. The core-shell Au-DTNB@Ag nanoparticles
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Toward nano-sized imprinted norepinephrine-derived biopolymer as artificial receptors for detecting IgG1 by surface plasmon resonance Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-15 Francesca Torrini, Giovanni Ferraro, Emiliano Fratini, Pasquale Palladino, Simona Scarano, Maria Minunni
Bio-based nanostructured molecularly imprinted polymers (nano-MIPs), also known as ‘plastibodies’, have a real potential to be used as alternatives to natural antibodies. These nanostructures have recently gained significant attention for diagnostic and therapeutic purposes. In this context, we have developed polynorepinephrine (PNE)-based nano-MIPs using an eco-friendly one-pot process for the sensitive
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Comparative analysis of electrochemical and optical sensors for detection of chronic wounds biomarkers: A review Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-15 Fátima A.R. Mota, Marieta L.C. Passos, João L.M. Santos, M.Lúcia M.F.S. Saraiva
Chronic wounds (CW) present a significant healthcare challenge due to their prolonged healing time and associated complications. To effectively treat these wounds and prevent further deterioration, monitoring their healing progress is crucial. Traditional wound assessment methods relying on visual inspection and subjective evaluation are prone to inter-observer variability. Biomarkers play a critical
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Novel high-dense microelectrode array based multimodal bioelectronic monitoring system for cardiac arrhythmia re-entry analysis Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-14 Sabine Schmidt, Wener Li, Mario Schubert, Björn Binnewerg, Christoph Prönnecke, Franziska D. Zitzmann, Martin Bulst, Sebastian Wegner, Matthias Meier, Kaomei Guan, Heinz-Georg Jahnke
In recent decades, significant progress has been made in the treatment of heart diseases, particularly in the field of personalized medicine. Despite the development of genetic tests, phenotyping and risk stratification are performed based on clinical findings and invasive techniques, such as stimulation conduction mapping techniques and programmed ventricular pacing. Consequently, label-free non-invasive
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A 3D-printed hollow microneedle-based electrochemical sensing device for in situ plant health monitoring Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-13 Marc Parrilla, Amadeo Sena-Torralba, Annemarijn Steijlen, Sergi Morais, Ángel Maquieira, Karolien De Wael
Plant health monitoring is devised as a new concept to elucidate physiological processes. The need for increased food production to nourish the growing global population is inconsistent with the dramatic impact of climate change, which hinders crop health and exacerbates plant stress. In this context, wearable sensors play a crucial role in assessing plant stress. Herein, we present a low-cost 3D-printed
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Digital monitoring of the microchannel filling flow dynamics using a non-contactless smartphone-based nano-liter precision flow velocity meter Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-13 Weiming Xu, Abdulkadir Yasin Atik, Levent Beker, Hatice Ceylan Koydemir
Microfluidic systems find widespread applications in diagnostics, biological research, chemistry, and engineering studies. Among their many critical parameters, flow rate plays a pivotal role in maintaining the functionality of microfluidic systems, including droplet-based microfluidic devices and those used in cell culture. It also significantly influences microfluidic mixing processes. Although various
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A cardiomyocyte-based biosensing platform for dynamic and quantitative investigation of excessive autophagy Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-13 Cheng-Wen He, Chunlian Qin, Yi Zhang, Yan Zhang, Kaiqiang Li, Yuqun Cai, Wei Zhang, Ning Hu, Zhen Wang
Autophagy is an important physiological phenomenon in eukaryotes that helps maintain the cellular homeostasis. Autophagy is involved in the development of various cardiovascular diseases, affecting the maintenance of cardiac function and disease prognosis. Physiological levels of autophagy serve as a defense mechanism for cardiomyocytes against environmental stimuli, but an overabundance of autophagy
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Allosteric strand displacement isothermal amplification for the visual detection of Toxoplasma gondii in 30 minutes Biosens. Bioelectron. (IF 12.6) Pub Date : 2024-02-13 Lei Wang, Zhaoxuan Zhang, Yanger Liu, Shenghao Lin, Wenqiang Zhang, Longjiao Zhu, Wentao Xu, Yulan Dong
Early detection of () is critical due to a lack of effective treatment for toxoplasmosis.This study established a simple, cost-effective, and rapid colorimetric detection method for The entire testing process, from sample collection to results, takes only 0.5 h. These characteristics fulfill the demands of researchers seeking rapid target detection with minimal equipment reliance. For genomic extraction