当前期刊: Journal of Food and Drug Analysis Go to current issue    加入关注   
显示样式:        排序: 导出
我的关注
我的收藏
您暂时未登录!
登录
  • Quantitation of DNA reactive pyrrolic metabolites of senecionine – A carcinogenic pyrrolizidine alkaloid by LC/MS/MS analysis
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-12-13
    Qingsu Xia; Xiaobo He; Qiang Shi; Ge Lin; Peter P. Fu

    Pyrrolizidine alkaloids (PAs) are carcinogenic phytochemicals, inducing liver tumors in experimental rodents. We previously determined that (±)-6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine (DHP), 7-glutathione-DHP, 7-cysteine-DHP, 7-N-acetylcysteine-DHP, and 1-CHO-DHP are DNA reactive pyrrolic metabolites potentially associated with PA-induced liver tumor initiation. In this study, we developed an LC/MS/MS multiple reaction monitoring (MRM) mode method to identify and quantify these metabolites formed from the metabolism of senecionine, a carcinogenic PA, by mouse, rat, and human liver microsomes, and primary rat hepatocytes. Together with the chemically prepared standards of these metabolites, this represents an accurate and convenient LC/MS/MS analytical method for quantifying these five reactive pyrrolic metabolites in biological systems.

    更新日期:2019-12-17
  • Healthy expectations of high hydrostatic pressure treatment in food processing industry
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-11-30
    Hsiao-Wen Huang, Chiao-Ping Hsu, Chung-Yi Wang
    更新日期:2019-11-30
  • Bioactive peptides attenuate cardiac hypertrophy and fibrosis in spontaneously hypertensive rat hearts
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-11-28
    Chih Yang Huang, Srinivasan Nithiyanantham, Jia Ying Liao, Wan Teng Lin
    更新日期:2019-11-29
  • In vitro and in vivo evaluation of the neuroprotective activity of Uncaria hirsuta Haviland
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-11-27
    Chien-Min Lin, Yi-Tzu Lin, Tai-Lin Lee, Zuha Imtiyaz, Wen-Chi Hou, Mei-Hsien Lee
    更新日期:2019-11-28
  • 更新日期:2019-11-28
  • 更新日期:2019-11-28
  • Recent advances in cancer chemoprevention with phytochemicals
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-11-25
    Yen-Chun Koh, Chi-Tang Ho, Min-Hsiung Pan

    Over the past few decades, phytochemicals widely present in edible plants have exhibited compelling positive biological impact on human health, including treating some cancers. In some cases, metabolites and artificially modified products of these natural compounds have shown better chemopreventive effects than their natural counterparts. Along with direct chemopreventive strategies using phytochemicals to treat cancer by leading to cell cycle arrest, autophagy and apoptosis, natural compounds have been shown to reverse adverse epigenetic regulation, including altering DNA methylation and histone modification, modulating miRNA expression, promoting expression of phase II enzyme for detoxification, balancing inflammation responses, recovering circadian rhythm from misalignment, and modifying gut microbiota. These have all become part of indirect but effective and novel strategies in cancer prevention using phytochemicals. Therefore, in this review, we are going to summarize some findings of phytochemicals in cancer chemoprevention via several distinct strategies, both to highlight promising treatments and to encourage new ideas for future studies.

    更新日期:2019-11-26
  • Analysis of potential anti-aging beverage Pru, a traditional Cuban refreshment, by desorption electrospray ionization-mass spectrometry and FTICR tandem mass spectrometry
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-06-27
    Md. Al Mamun, Tania Valdes Gonzalez, Ariful Islam, Tomohito Sato, Shumpei Sato, Takashi K. Ito, Makoto Horikawa, Fumiyoshi Yamazaki, Rolando Contreras Alarcon, Tatsuo Ido, Mitsutoshi Setou
    更新日期:2019-11-18
  • Distribution of antibiotic resistance genes among Staphylococcus species isolated from ready-to-eat foods
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-06-27
    Yu-Ting Wang, Yu-Tzu Lin, Tsai-Wen Wan, Der-Yuan Wang, Hsu-Yang Lin, Che-Yang Lin, Yu-Chih Chen, Lee-Jene Teng
    更新日期:2019-11-18
  • Viola cornuta and Viola x wittrockiana: Phenolic compounds, antioxidant and neuroprotective activities on Caenorhabditis elegans
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-07-02
    Cristina Moliner, Lillian Barros, Maria Inês Dias, Inés Reigada, Isabel C.F.R. Ferreira, Víctor López, Elisa Langa, Carlota Gómez Rincón
    更新日期:2019-11-18
  • Efficient identification of fungal antimicrobial principles by tandem MS and NMR database
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-06-27
    Ming-Shian Lee, Yu-Liang Yang, Chia-Yen Wu, Ying-Lien Chen, Ching-Kuo Lee, Shean-Shong Tzean, Tzong-Huei Lee
    更新日期:2019-11-18
  • Impact of dietary ingredients on the interpretation of various fecal parameters in rats fed inulin
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-07-16
    Hui-Ju Chen, Fan-Jhen Dai, Chih-Ren Chang, Yie-Qie Lau, Boon-Swee Chew, Chi-Fai Chau
    更新日期:2019-11-18
  • A low cost smart system to analyze different types of edible Bird's nest adulteration based on colorimetric sensor array
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-07-16
    Xiaowei Huang, Zhihua Li, Xiaobo Zou, Jiyong Shi, Haroon Elrasheid Tahir, Yiwei Xu, Xiaodong Zhai, Xuetao Hu
    更新日期:2019-11-18
  • 更新日期:2019-11-18
  • Simultaneous determination of cardiovascular drugs in dried blood spot by liquid chromatography-tandem mass spectrometry
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-06-26
    Hyung Min Kim, Ju-Hwan Park, Nguyen Phuoc Long, Dae-Duk Kim, Sung Won Kwon
    更新日期:2019-11-18
  • Mulberry fruits extracts induce apoptosis and autophagy of liver cancer cell and prevent hepatocarcinogenesis in vivo
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-06-27
    Kwok-Chui Cheng, Chau-Jong Wang, Yun-Ching Chang, Tung-Wei Hung, Chun-Jung Lai, Chi-Wen Kuo, Hui-Pei Huang
    更新日期:2019-11-18
  • Biotransformation of mogrosides from Siraitia grosvenorii by Ganoderma lucidum mycelium and the purification of mogroside III E by macroporous resins
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-05-29
    Chun-Hui Chiu, Reuben Wang, Shasha Zhuang, Pei-Yin Lin, Yi-Chen Lo, Ting-Jang Lu
    更新日期:2019-11-18
  • Preface.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2018-04-29
    Pei-Dawn Lee Chao

    更新日期:2019-11-01
  • Recent progress in natural dietary non-phenolic bioactives on cancers metastasis.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2018-07-07
    Gow-Chin Yen,Chiung-Man Tsai,Chi-Cheng Lu,Chia-Jui Weng

    From several decades ago to now, cancer continues to be the leading cause of death worldwide, and metastasis is the major cause of cancer-related deaths. For health benefits, there is a great desire to use non-chemical therapy such as nutraceutical supplementation to prevent pathology development. Over 10,000 different natural bioactives or phytochemicals have been known that possessing potential preventive or supplementary effects for various diseases including cancer. Previously, the in vitro and in vivo anti-invasive and anti-metastatic activities of phenolic acids, monophenol, polyphenol and their derivatives and flavonoids and their derivatives have been reviewed. However, a vast number of natural dietary compounds other than phenolics have been demonstrated to potentially possess the ability to inhibit the invasion and metastasis of various cancers. In this review, we summarize the studies in recent decade on in vitro and in vivo effects and molecular mechanisms of natural bioactives, excluding the phenolics in food, in cancer invasion and metastasis. By combining this review of non-phenolics with the previous phenolics reviews, the puzzle for the contribution of natural dietary bioactives on cancer invasive or/and metastatic progress will be almost complete and more clear.

    更新日期:2019-11-01
  • Corrigendum to "Investigation of borneols sold in Taiwan by chiral gas chromatography" [J Food Drug Anal 26 (2018) 348-352].
    J. Food Drug Anal. (IF 4.176) Pub Date : 2019-01-17
    Tsung-Jung Ho,Chien-Che Hung,Tzenge-Lien Shih,Lih-Ming Yiin,Hao-Ping Chen

    更新日期:2019-11-01
  • Chemistry and health effects of furanocoumarins in grapefruit.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-09-16
    Wei-Lun Hung,Joon Hyuk Suh,Yu Wang

    Furanocoumarins are a specific group of secondary metabolites that commonly present in higher plants, such as citrus plants. The major furanocoumarins found in grapefruits (Citrus paradisi) include bergamottin, epoxybergamottin, and 6',7'-dihydroxybergamottin. During biosynthesis of these furanocoumarins, coumarins undergo biochemical modifications corresponding to a prenylation reaction catalyzed by the cytochrome P450 enzymes with the subsequent formation of furan rings. Because of undesirable interactions with several medications, many studies have developed methods for grapefruit furanocoumarin quantification that include high-performance liquid chromatography coupled with UV detector or mass spectrometry. The distribution of furanocoumarins in grapefruits is affected by several environmental conditions, such as processing techniques, storage temperature, and packing materials. In the past few years, grapefruit furanocoumarins have been demonstrated to exhibit several biological activities including antioxidative, -inflammatory, and -cancer activities as well as bone health promotion both in vitro and in vivo. Notably, furanocoumarins potently exerted antiproliferative activities against cancer cell growth through modulation of several molecular pathways, such as regulation of the signal transducer and activator of transcription 3, nuclear factor-κB, phosphatidylinositol-3-kinase/AKT, and mitogen-activated protein kinase expression. Therefore, based on this review, we suggest furanocoumarins may serve as bioactive components that contribute, at least in part, to the health benefits of grapefruit.

    更新日期:2019-11-01
  • Assessment of the toxic potential of graphene family nanomaterials.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Xiaoqing Guo,Nan Mei

    Graphene, a single-atom-thick carbon nanosheet, has attracted great interest as a promising nanomaterial for a variety of bioapplications because of its extraordinary properties. However, the potential for widespread human exposure raises safety concerns about graphene and its derivatives, referred to as graphene-family nanomaterials. This review summarizes recent findings on the toxicological effects and the potential toxicity mechanisms of graphene-family nanomaterials in bacteria, mammalian cells, and animal models. Graphene, graphene oxide, and reduced graphene oxide elicit toxic effects both in vitro and in vivo, whereas surface modifications can significantly reduce their toxic interactions with living systems. Standardization of terminology and the fabrication methods of graphene-family nanomaterials are warranted for further investigations designed to decrease their adverse effects and explore their biomedical applications.

    更新日期:2019-11-01
  • Corrigendum to "Chemopreventive effect of natural dietary compounds on xenobiotic-induced toxicity" [J Food Drug Anal 25 (2017) 176-186].
    J. Food Drug Anal. (IF 4.176) Pub Date : 2018-02-02
    Jia-Ching Wu,Ching-Shu Lai,Mei-Ling Tsai,Chi-Tang Ho,Ying-Jan Wang,Min-Hsiung Pan

    更新日期:2019-11-01
  • 更新日期:2019-11-01
  • Corrigendum to "Biopharmaceutical potentials of Prosopis spp. (Mimosaceae, Leguminosa)" [J Food Drug Anal 25 (2017) 187-196].
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-09-16
    Santhaseelan Henciya,Prabha Seturaman,Arthur Rathinam James,Yi-Hong Tsai,Rahul Nikam,Yang-Chang Wu,Hans-Uwe Dahms,Fang Rong Chang

    更新日期:2019-11-01
  • Evaluation of the prebiotic effects of citrus pectin hydrolysate.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-09-16
    Yen-Yi Ho,Chia-Min Lin,Ming-Chang Wu

    Citrus pectin enzyme hydrolysate (PEH) of different hydrolysis time intervals (6 hours, PEH-6; 12 hours, PEH-12; 24 hours, PEH-24; or 48 hours, PEH-48) or concentrations (1%, 2%, and 4%) was tested for its growth stimulation effect on two probiotics, Bifidobacterium bifidum and Lactobacillus acidophilus. Higher monosaccharide concentrations and smaller molecular weights of PEHs were obtained by prolonging the hydrolysis time. In addition, higher PEH concentrations resulted in significantly higher (p < 0.05) probiotic populations, pH reduction, and increase in total titratable acidity than the glucose-free MRS negative control. Furthermore, significantly higher populations in the low pH environment and longer survival time in nonfat milk (p < 0.05) were observed when the two probiotics were incubated in media supplemented with 2% PEH-24, than in glucose and the negative control. In comparison with other prebiotics, addition of 2% PEH-24 resulted in a more significant increase in the probiotic population (p < 0.05) than in the commercial prebiotics. This study demonstrated that PEH derived from citrus pectin could be an effective prebiotic to enhance the growth, fermentation, acid tolerance, and survival in nonfat milk for the tested probiotics.

    更新日期:2019-11-01
  • High-throughput liquid chromatography tandem mass spectrometry method for simultaneous determination of fampridine, paroxetine, and quinidine in rat plasma: Application to in vivo perfusion study.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-09-16
    Suneetha Achanti,Raja Rajeswari Katta

    A selective and high-throughput liquid chromatography-mass spectrometry method has been developed and validated for the simultaneous quantification of paroxetine, fampridine, and quinidine in rat plasma using imipramine as an internal standard. Following protein precipitation extraction, the analytes and internal standard were run on XBridge C18 column (150 mm × 4.6 mm, 5 μm) using a gradient mobile phase consisting of 5mM ammonium formate in water (pH 9.0) and acetonitrile in a flow gradience program. The precursor and product ions of the drugs were monitored on a triple quadrupole instrument operated in the positive ionization mode. The method was validated over a concentration range of 0.1-100 ng/mL for all the three analytes, with relative recoveries ranging from 69% to 82%. The intra- and interbatch precision (percent coefficient of variation) across four validation runs were less than 13.4%. The accuracy determined at four quality control (QC) levels (lower limit of quantitation, low QC, medium QC, and high QC) was within ±6.5% of coefficient of variation values. The method proved highly reproducible and sensitive, and was successfully applied in a pharmacokinetic study after single-dose oral administration to rats and also in perfusion study sample analysis.

    更新日期:2019-11-01
  • Effect of shaking process on correlations between catechins and volatiles in oolong tea.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2016-07-01
    Shu-Yen Lin,Li-Chiao Lo,Iou-Zen Chen,Po-An Chen

    Shaking the tea leaves is the key manipulation to making oolong tea. It contributes to the formation of flavor and fragrance in oolong tea. The dynamic variations of catechins and volatile organic compounds (VOCs) during the shaking process were investigated. The results showed that the contents of epicatechin, epigallocatechin, epicatechin gallate (ECG), and epigallocatechin gallate (EGCG) first decreased after the shaking and then increased to the initial value before the next shaking. Geraniol, linalool and its oxides, and phenylethyl alcohol showed similar variations. The contents of trans-β-ocimene, 1H-indole, and 3-hexenyl hexanoate increased after the second or third shaking (the late fermentation stage). However, the contents of aldehydes showed an opposite trend to other VOCs. The abundance of phenylethyl alcohol was positively related to the content of ECG and EGCG during fermentation, whereas the abundance of cis-3-hexenal was negatively related to the content of ECG. The correlations between catechin and VOCs indicated that shaking affected the chemical transformation of the compounds in oolong tea.

    更新日期:2019-11-01
  • Biopharmaceutical potentials of Prosopis spp. (Mimosaceae, Leguminosa).
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-09-16
    Santhaseelan Henciya,Prabha Seturaman,Arthur Rathinam James,Yi-Hong Tsai,Rahul Nikam,Yang-Chang Wu,Hans-Uwe Dahms,Fang Rong Chang

    Prosopis is a commercially important plant genus, which has been used since ancient times, particularly for medicinal purposes. Traditionally, Paste, gum, and smoke from leaves and pods are applied for anticancer, antidiabetic, anti-inflammatory, and antimicrobial purposes. Components of Prosopis such as flavonoids, tannins, alkaloids, quinones, or phenolic compounds demonstrate potentials in various biofunctions, such as analgesic, anthelmintic, antibiotic, antiemetic, microbial antioxidant, antimalarial, antiprotozoal, antipustule, and antiulcer activities; enhancement of H+, K+, ATPases; oral disinfection; and probiotic and nutritional effects; as well as in other biopharmaceutical applications, such as binding abilities for tablet production. The compound juliflorine provides a cure in Alzheimer disease by inhibiting acetylcholine esterase at cholinergic brain synapses. Some indirect medicinal applications of Prosopis spp. are indicated, including antimosquito larvicidal activity, chemical synthesis by associated fungal or bacterial symbionts, cyanobacterial degradation products, "mesquite" honey and pollens with high antioxidant activity, etc. This review will reveal the origins, distribution, folk uses, chemical components, biological functions, and applications of different representatives of Prosopis.

    更新日期:2019-11-01
  • Prevention, family, and community.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-09-30
    Shu-Lung Yang,Louise Ann Rohrbach,Dennis Daley

    The "Prevention, Family, and Community" session was chaired by Dr. Joseph Jror-Serk Cheng, who is an expert in community psychiatry and mental health policy and is superintendent of the Bali Psychiatric Center in Taipei. Dr. Shu-Lung Yang, dean of Student Affairs and Professor/Director of the Crime Research Center, National Chung Cheng University in Taiwan, served as the discussant. The two presenters were Dr. Louise Ann Rohrbach, who presented on "Prevention of Alcohol and other Drug Abuse: Science, Practice, Critical Issues, and Future Direction," and Dr. Dennis Daley, who spoke on "Family and Social Aspects of Drug Abuse: Implications for Treatment and Recovery." Dr. Rohrbach is associate professor of Preventive Medicine and director of the Master of Public Health (MPH) program at the University of Southern California (USC) Keck School of Medicine. Dr. Daley is professor of psychiatry at the University of Pittsburgh School of Medicine in Pennsylvania.

    更新日期:2019-11-01
  • Molecular toxicity mechanism of nanosilver.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Danielle McShan,Paresh C Ray,Hongtao Yu

    Silver is an ancient antibiotic that has found many new uses due to its unique properties on the nanoscale. Due to its presence in many consumer products, the toxicity of nanosilver has become a hot topic. This review summarizes recent advances, particularly the molecular mechanism of nanosilver toxicity. The surface of nanosilver can easily be oxidized by O(2) and other molecules in the environmental and biological systems leading to the release of Ag(+), a known toxic ion. Therefore, nanosilver toxicity is closely related to the release of Ag(+). In fact, it is difficult to determine what portion of the toxicity is from the nano-form and what is from the ionic form. The surface oxidation rate is closely related to the nanosilver surface coating, coexisting molecules, especially thiol-containing compounds, lighting conditions, and the interaction of nanosilver with nucleic acids, lipid molecules, and proteins in a biological system. Nanosilver has been shown to penetrate the cell and become internalized. Thus, nanosilver often acts as a source of Ag(+) inside the cell. One of the main mechanisms of toxicity is that it causes oxidative stress through the generation of reactive oxygen species and causes damage to cellular components including DNA damage, activation of antioxidant enzymes, depletion of antioxidant molecules (e.g., glutathione), binding and disabling of proteins, and damage to the cell membrane. Several major questions remain to be answered: (1) the toxic contribution from the ionic form versus the nano-form; (2) key enzymes and signaling pathways responsible for the toxicity; and (3) effect of coexisting molecules on the toxicity and its relationship to surface coating.

    更新日期:2019-11-01
  • 更新日期:2019-11-01
  • Exploration of ethyl anthranilate-loaded monolithic matrix-type prophylactic polymeric patch.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Johirul Islam,Kamaruz Zaman,Srijita Chakrabarti,Nilutpal Sharma Bora,Manash Pratim Pathak,Santa Mandal,Julfikar Ali Junejo,Pronobesh Chattopadhyay

    Compromised stability of pharmaceutical formulations loaded with volatiles is a serious problem associated with devices designed to deliver volatile compounds. The present study has been focused to evaluate the stability potential of matrix-type polymeric patches composed of volatile ethyl anthranilate for prophylaxis against vector-borne diseases. Ethyl anthranilate-loaded matrix-type polymeric patches were fabricated by solvent evaporation method on an impermeable backing membrane and attached to temporary release liners. Stability testing of the polymeric patches was performed as per the International Conference on Harmonization (ICH) guidelines for 6 months under accelerated conditions. In addition, the quantification of residual solvents was also performed as per the ICH guidelines. After conducting the stability studies for 6 months, the optimized patches showed the best possible results with respect to uniformity of drug content, physical appearance, and other analytical parameters. Furthermore, the amount of residual solvent was found well below the accepted limit. Thus, the present report outlined the analytical parameters to be evaluated to ensure the stability of a certain devices consisting of volatile compounds.

    更新日期:2019-11-01
  • Neurotoxicity of nanoscale materials.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Alokita Karmakar,Qinli Zhang,Yongbin Zhang

    Nanotechnology has been applied in consumer products and commercial applications, showing a significant impact on almost all industries and all areas of society. Significant evidence indicates that manufactured nanomaterials and combustion-derived nanomaterials elicit toxicity in humans exposed to these nanomaterials. The interaction of the engineered nanomaterials with the nervous system has received much attention in the nanotoxicology field. In this review, the biological effects of metal, metal oxide, and carbon-based nanomaterials on the nervous system are discussed from both in vitro and in vivo studies. The translocation of the nanoparticles through the blood-brain barrier or nose to brain via the olfactory bulb route, oxidative stress, and inflammatory mechanisms of nanomaterials are also reviewed.

    更新日期:2019-11-01
  • Using a holistic approach to assess the impact of engineered nanomaterials inducing toxicity in aquatic systems.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Xiaojia He,Winfred G Aker,Jerzy Leszczynski,Huey-Min Hwang

    In this report, we critically reviewed selected intrinsic physicochemical properties of engineered nanomaterials (ENMs) and their role in the interaction of the ENMs with the immediate surroundings in representative aquatic environments. The behavior of ENMs with respect to dynamic microenvironments at the nano-bio-eco interface level, and the resulting impact on their toxicity, fate, and exposure potential are elaborated. Based on this literature review, we conclude that a holistic approach is urgently needed to fulfill our knowledge gap regarding the safety of discharged ENMs. This comparative approach affords the capability to recognize and understand the potential hazards of ENMs and their toxicity mechanisms, and ultimately to establish a quantitative and reliable system to predict such outcomes.

    更新日期:2019-11-01
  • Genotoxicity of titanium dioxide nanoparticles.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Tao Chen,Jian Yan,Yan Li

    Titanium dioxide nanoparticles (TiO(2)-NPs, <100 nm) are increasingly being used in pharmaceuticals and cosmetics due to the unique properties derived from their small sizes. However, their large surface-area to mass ratio and high redox potential may negatively impact human health and the environment. TiO(2)-NPs can cause inflammation, pulmonary damage, fibrosis, and lung tumors and they are possibly carcinogenic to humans. Because cancer is a disease involving mutation, there are a large number of studies on the genotoxicity of TiO(2)-NPs. In this article, we review the results that have been reported in the literature, with a focus on data generated from the standard genotoxicity assays. The data include genotoxicity results from the Ames test, in vitro and in vivo Comet assay, in vitro and in vivo micronucleus assay, sister chromatid exchange assay, mammalian cell hypoxanthine-guanine phosphoribosyl transferase gene assay, the wing somatic mutation and recombination assay, and the mouse phosphatidylinositol glycan, class A gene assay. Inconsistent results have been found in these assays, with both positive and negative responses being reported. The in vitro systems for assessing the genotoxicity of TiO(2)-NPs have generated a greater number of positive results than the in vivo systems, and tests for DNA and chromosome damage have produced more positive results than the assays measuring gene mutation. Nearly all tests for measuring the mutagenicity of TiO(2)-NPs were negative. The current data indicate that the genotoxicity of TiO(2)-NPs is mediated mainly through the generation of oxidative stress in cells.

    更新日期:2019-11-01
  • Reactive oxygen species-related activities of nano-iron metal and nano-iron oxides.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Haohao Wu,Jun-Jie Yin,Wayne G Wamer,Mingyong Zeng,Y Martin Lo

    Nano-iron metal and nano-iron oxides are among the most widely used engineered and naturally occurring nanostructures, and the increasing incidence of biological exposure to these nanostructures has raised concerns about their biotoxicity. Reactive oxygen species (ROS)-induced oxidative stress is one of the most accepted toxic mechanisms and, in the past decades, considerable efforts have been made to investigate the ROS-related activities of iron nanostructures. In this review, we summarize activities of nano-iron metal and nano-iron oxides in ROS-related redox processes, addressing in detail the known homogeneous and heterogeneous redox mechanisms involved in these processes, intrinsic ROS-related properties of iron nanostructures (chemical composition, particle size, and crystalline phase), and ROS-related bio-microenvironmental factors, including physiological pH and buffers, biogenic reducing agents, and other organic substances.

    更新日期:2019-11-01
  • Mechanistic characterization of titanium dioxide nanoparticle-induced toxicity using electron spin resonance.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Meng Li,Jun-Jie Yin,Wayne G Wamer,Y Martin Lo

    Titanium dioxide nanoparticles (TiO(2) NPs) are one of the most widely used nanomaterials that have been manufactured worldwide and applied in different commercial realms. The well-recognized ability of TiO(2) to promote the formation of reactive oxygen species (ROS) has been extensively studied as one of the important mechanisms underlying TiO(2) NPs toxicity. As the "gold standard" method to quantify and identify ROS, electron spin resonance (ESR) spectroscopy has been employed in many studies aimed at evaluating TiO(2) NPs safety. This review aims to provide a thorough discussion of current studies using ESR as the primary method to unravel the mechanism of TiO(2) NPs toxicity. ESR spin label oximetry and immune-spin trapping techniques are also briefly introduced, because the combination of spin trapping/labeling techniques offers a promising tool for studying the oxidative damage caused by TiO(2) NPs.

    更新日期:2019-11-01
  • Mechanisms of nanotoxicity: generation of reactive oxygen species.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Peter P Fu,Qingsu Xia,Huey-Min Hwang,Paresh C Ray,Hongtao Yu

    Nanotechnology is a rapidly developing field in the 21(st) century, and the commercial use of nanomaterials for novel applications is increasing exponentially. To date, the scientific basis for the cytotoxicity and genotoxicity of most manufactured nanomaterials are not understood. The mechanisms underlying the toxicity of nanomaterials have recently been studied intensively. An important mechanism of nanotoxicity is the generation of reactive oxygen species (ROS). Overproduction of ROS can induce oxidative stress, resulting in cells failing to maintain normal physiological redox-regulated functions. This in turn leads to DNA damage, unregulated cell signaling, change in cell motility, cytotoxicity, apoptosis, and cancer initiation. There are critical determinants that can affect the generation of ROS. These critical determinants, discussed briefly here, include: size, shape, particle surface, surface positive charges, surface-containing groups, particle dissolution, metal ion release from nanometals and nanometal oxides, UV light activation, aggregation, mode of interaction with cells, inflammation, and pH of the medium.

    更新日期:2019-11-01
  • Electron spin resonance spectroscopy for the study of nanomaterial-mediated generation of reactive oxygen species.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Weiwei He,Yitong Liu,Wayne G Wamer,Jun-Jie Yin

    Many of the biological applications and effects of nanomaterials are attributed to their ability to facilitate the generation of reactive oxygen species (ROS). Electron spin resonance (ESR) spectroscopy is a direct and reliable method to identify and quantify free radicals in both chemical and biological environments. In this review, we discuss the use of ESR spectroscopy to study ROS generation mediated by nanomaterials, which have various applications in biological, chemical, and materials science. In addition to introducing the theory of ESR, we present some modifications of the method such as spin trapping and spin labeling, which ultimately aid in the detection of short-lived free radicals. The capability of metal nanoparticles in mediating ROS generation and the related mechanisms are also presented.

    更新日期:2019-11-01
  • Raman spectroscopy in the analysis of food and pharmaceutical nanomaterials.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Ying-Sing Li,Jeffrey S Church

    Raman scattering is an inelastic phenomenon. Although its cross section is very small, recent advances in electronics, lasers, optics, and nanotechnology have made Raman spectroscopy suitable in many areas of application. The present article reviews the applications of Raman spectroscopy in food and drug analysis and inspection, including those associated with nanomaterials. Brief overviews of basic Raman scattering theory, instrumentation, and statistical data analysis are also given. With the advent of Raman enhancement mechanisms and the progress being made in metal nanomaterials and nanoscale metal surfaces fabrications, surface enhanced Raman scattering spectroscopy has become an extra sensitive method, which is applicable not only for analysis of foods and drugs, but also for intracellular and intercellular imaging. A Raman spectrometer coupled with a fiber optics probe has great potential in applications such as monitoring and quality control in industrial food processing, food safety in agricultural plant production, and convenient inspection of pharmaceutical products, even through different types of packing. A challenge for the routine application of surface enhanced Raman scattering for quantitative analysis is reproducibility. Success in this area can be approached with each or a combination of the following methods: (1) fabrication of nanostructurally regular and uniform substrates; (2) application of statistic data analysis; and (3) isotopic dilution.

    更新日期:2019-11-01
  • Cell microenvironment stimuli-responsive controlled-release delivery systems based on mesoporous silica nanoparticles.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Chun-Ling Zhu,Xian-Wei Wang,Zhen-Zhen Lin,Zeng-Hong Xie,Xiao-Ru Wang

    To develop novel tumor cell microenvironment stimuli-responsive smart controlled-release delivery systems is one of the current common interests of materials science and clinical medicine. Meanwhile, mesoporous silica nanoparticles as a promising drug carrier have become the new area of interest in the field of biomedical application in recent years because of their unique characteristics and abilities to efficiently and specifically entrap cargo molecules. This review describes the more recent developments and achievements of mesoporous silica nanoparticles in drug delivery. In particular, we focus on the stimuli-responsive controlled-release systems that are able to respond to tumor cell environmental changes, such as pH, glucose, adenosine-5'-triphosphate (ATP), glutathione (GSH), and H(2)O(2).

    更新日期:2019-11-01
  • Theranostic nanomedicine for cancer detection and treatment.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-03-29
    Zhen Fan,Peter P Fu,Hongtao Yu,Paresh C Ray

    Cancer is the second leading cause of death in the USA according to the American Cancer Society. In the past 5 years, "theranostic nanomedicine", for both therapeutics and imaging, has shown to be "the right drug for the right patient at the right moment" to manage deadly cancers. This review article presents an overview of recent developments, mainly from the authors' laboratories, along with potential medical applications for theranostic nanomedicine including basic concepts and critical properties. Finally, we outline the future research direction and possible challenges for theranostic nanomedicine research.

    更新日期:2019-11-01
  • 更新日期:2019-11-01
  • Capacity building and collaborative research on cross-national studies in the Asian region.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-02-26
    Yih-Ing Hser,Linda Chang,Gene-Jack Wang,Ming D Li,Richard Rawson,Steve Shoptaw,Jacques Normand,Betty Tai

    To build capacity and collaborative research for future cross-national studies in the Asian and Pacific Islander (API) region, priority research topics were identified and discussed at the April 2013 Conference to Promote Global Health in Taipei. These topics included (1) Neuroscience on HIV/HCV and amphetamine-type stimulants (ATS), led by Drs. Linda Chang, Gene-Jack Wang, and Betty Tai; (2) ATS and mental health disorders, led by Drs. Richard Rawson and Wilson Compton; and (3) HIV/HCV transmission and social networks, led by Drs. Steven Shoptaw and Jacques Normand. Potential genetic studies spanning these topical areas as well as the importance of smoking cessation were further discussed, led by Dr. Ming Li. Additional priority research topics were also identified: (4) Drug use prevention, and (5) Family involvement to improve treatment adherence and recovery. Workgroups on these topics will be formed to prioritize research questions within the respective topical area and to determine the next steps. The ultimate goal of these workgroups is to stimulate collaboration that will eventually lead to research studies addressing critical issues related to the rising substance abuse and HIV infection rates in many Asian countries and, at the same time, to advance the scientific knowledge of substance abuse and HIV infection.

    更新日期:2019-11-01
  • Medication-assisted treatment for opioid addiction: methadone and buprenorphine.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-01-18
    Andrew J Saxon,Yih-Ing Hser,George Woody,Walter Ling

    Among agents for treatment of opioid addiction, methadone is a full mu-opioid receptor agonist, whereas buprenorphine is a partial agonist. Both are long-acting. Buprenorphine has a superior safety profile. Methadone is formulated for oral administration and buprenorphine for sublingual administration. A subdermal buprenorphine implant with a 6-month duration of action is being considered for approval by the U.S. Food and Drug Administration. Both medications reduce mortality rates and improve other outcomes. Data from a recent randomized controlled comparison of both medications (N = 1269) show better treatment retention with methadone but reduced illicit opioid use early in treatment with buprenorphine. Human immunodeficiency virus (HIV) risk behaviors were measured using the Risk Behavior Survey at baseline, 12 weeks, and 24 weeks for study completers. In the 30 days prior to treatment entry, 14.4% of the completers randomized to treatment with buprenorphine (n = 340) and 14.1% of the completers randomized to methadone treatment (n = 391) shared needles. The percent sharing needles decreased to 2.4% for buprenorphine and 4.8 for methadone in the 30 days prior to Week 24 (p < 0.0001). In the 30 days prior to treatment entry, 6.8% of the completers randomized to buprenorphine and 8.2% of the completers randomized to methadone had multiple sexual partners, with only 5.2% and 5.1%, respectively, reporting multiple partners at Week 24 (p < 0.04).

    更新日期:2019-11-01
  • Autophagy in drug-induced liver toxicity.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2014-06-01
    Si Chen,William B Melchior,Yuanfeng Wu,Lei Guo

    Liver injury resulting from exposure to drugs and chemicals is a major health problem. Autophagy is an important factor in a wide range of diseases, such as cancer, liver disease, muscular disorder, neurodegeneration, pathogen infection, and aging, and emerging evidence indicates that autophagy makes a substantial contribution to the pathogenesis of drug- and chemical-induced liver toxicity. In this review, we summarize current knowledge on autophagy triggered by toxicants/toxins, the protective role of autophagy in liver toxicity, and the underlying molecular mechanisms. We also highlight experimental approaches for studying autophagy.

    更新日期:2019-11-01
  • Analysis of bioactive constituents from the leaves of Amorpha fruticosa L.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Xueqin Cui,Jing Guo,Ching-Shu Lai,Min-Hsiung Pan,Zhongxiao Ma,Sen Guo,Qingchao Liu,Li Zhang,Chi-Tang Ho,Naisheng Bai

    Amorpha fruticosa L. is a Chinese folk medicine and rich in polyphenols. Fifteen known compounds were isolated and identified from the leaves of A. fruticosa L. They are tephrosin (1), 6a,12a-dehydrodeguelin (2), vitexin (3), afrormosin-7-O-β-d-glucopyranoside (4), 2″-O-α-l-rhamnopyranosyl isovitexin (5), rutin (6), chrysoeriol (7), 7-O-methylluteolin (8), trans-p-coumaric acid (9), 2-benzyl-4,6-dihydroxybenzoic acid-4-O-β-d-glucopyranoside (10), formononetin (11), quercetin (12), apigenin (13), β-sitosterol (14), and β-daucosterol (15). Compounds 3, 4, 5, and 7-9 were isolated from A. fruticosa L. for the first time. Cytotoxicity of individual compounds 3-10 and 90% ethanol extract against human cancer cell lines HCT116 and HepG2 were reported. The results suggested that compounds 7 and 8, and the crude extract exhibited inhibitory effects on human cancer cell line HCT116, at concentrations of 100 μg/mL, 5 μg/mL, and 25 μg/mL at <60% of cell viability rate, respectively. In addition, a valid high-performance liquid chromatography diode array detector method was established to quantitatively analyze compounds 1-12 in the leaves of A. fruticosa L., which was harvested at three different stages of maturity from May 20 to August 10, 2014. The results demonstrated that contents were greatly influenced by the maturity. Total amounts of the analytical constituents gradually increased from May 20 to August 10, with the values ranging from 10.86 mg/g to 18.84 mg/g, whereas bioactive compounds 7 and 8 presented the opposite variation trend. The results of this study may provide data for further study and comprehensive utilization of A. fruticosa L. RESOURCE

    更新日期:2019-11-01
  • Pyrrolizidine alkaloid-derived DNA adducts are common toxicological biomarkers of pyrrolizidine alkaloid N-oxides.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Xiaobo He,Qingsu Xia,Kellie Woodling,Ge Lin,Peter P Fu

    There are 660 pyrrolizidine alkaloids (PAs) and PA N-oxides present in the plants, with approximately half being possible carcinogens. We previously reported that a set of four PA-derived DNA adducts is formed in the liver of rats administered a series of hepatocarcinogenic PAs and a PA N-oxide. Based on our findings, we hypothesized that this set of DNA adducts is a common biological biomarker of PA-induced liver tumor formation. In this study, we determined that rat liver microsomal metabolism of five hepatocarcinogenic PAs (lasiocarpine, retrorsine, riddelliine, monocrotaline, and heliotrine) and their corresponding PA N-oxides produced the same set of DNA adducts. Among these compounds, lasiocarpine N-oxide, retrorsine N-oxide, monocrotaline N-oxide, and heliotrine N-oxide are for first time shown to be able to produce these DNA adducts. These results further support the role of these DNA adducts as potential common biomarkers of PA-induced liver tumor initiation.

    更新日期:2019-11-01
  • Phosphorylation and antiaging activity of polysaccharide from Trichosanthes peel.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Min Zhang,Nana Su,Qianli Huang,Qiang Zhang,Yufen Wang,Jinglei Li,Ming Ye

    Polysaccharides from Trichosanthes peel (TPP) were obtained by ultrasound-assisted extraction. TPP-1 was separated from the TPP by Sephadex G-100 column chromatography. Phosphorylation of TPP-1 was carried out and phosphorylated TPP-1 was named as PTTP-1. The results of infrared spectra, 13C nuclear magnetic resonance spectra and 31P nuclear magnetic resonance spectra showed that the main structure of PTPP-1 was similar to that of TPP-1 and -H2PO3 groups which were conjugated to C-6 of →4)-α-D-Manp-(1→, C-4 of →6)-α-D-Galp-(1→, C-2 and C-3 of →1)-α-L-Araf, C-2 of →1)-α-L-Araf-(3→, and C-6 and C-3 of →1)-α-D-Glcp. In vivo antiaging activity results proved that TTP-1 and PTTP-1 could both significantly improve the body weight, spleen index, and thymus index of the D-galactose-induced aging mice, increase the levels of superoxide dismutase, catalase, glutathione peroxidase, and reduce malondialdehyde contents in the liver, brain, and serum of aging mice. These results indicated that both TPP-1 and PTTP-1 presented significant antiaging activity. Moreover, PTTP-1 showed stronger antiaging effects in aging mice, indicating that phosphorylation improved antiaging effect.

    更新日期:2019-11-01
  • Quality assessment of trace Cd and Pb contaminants in Thai herbal medicines using ultrasound-assisted digestion prior to flame atomic absorption spectrometry.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Watsaka Siriangkhawut,Patcharee Sittichan,Kraingkrai Ponhong,Piyanete Chantiratikul

    A simple, efficient, and reliable ultrasound-assisted digestion (UAD) procedure was used for sample preparation prior to quantitative determination of trace Cd and Pb contaminants in herbal medicines using flame atomic absorption spectrometry. The parameters influencing UAD such as the solvent system, sample mass, presonication time, sonication time, and digestion temperature were evaluated. The efficiency of the proposed UAD procedure was evaluated by comparing with conventional acid digestion (CAD) procedure. Under the optimum conditions, linear calibration graphs in a range of 2-250 μg/L for Cd, and 50-1000 μg/L for Pb were obtained with detection limits of 0.56 μg/L and 10.7 μg/L for Cd and Pb, respectively. The limit of quantification for Cd and Pb were 1.87 μg/L and 40.3 μg/L, respectively. The repeatability for analysis of 10 μg/L for Cd and 100 μg/L for Pb was 2.3% and 2.6%, respectively. The accuracy of the proposed method was evaluated by rice flour certified reference materials. The proposed method was successfully applied for analysis of trace Cd and Pb in samples of various types of medicinal plant and traditional medicine consumed in Thailand. Most herbal medicine samples were not contaminated with Cd or Pb. The contaminant levels for both metals were still lower than the maximum permissible levels of elements in medicinal plant materials and finished herbal products sets by the Ministry of Public Health of Thailand. The exception was the high level of Cd contamination found in two samples of processed medicinal plants.

    更新日期:2019-11-01
  • Qualitative and quantitative characterization of secondary metabolites and carbohydrates in Bai-Hu-Tang using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and ultraperformance liquid chromatography coupled with photodiode array detector.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Wei-Fang Zhong,Wing-Sum Tong,Shan-Shan Zhou,Ka-Man Yip,Song-Lin Li,Zhong-Zhen Zhao,Jun Xu,Hu-Biao Chen

    Bai-Hu-Tang (BHT), a classic traditional Chinese medicine (TCM) formula used for clearing heat and promoting body fluid, consists of four traditional Chinese medicines, i.e., Gypsum Fibrosum (Shigao), Anemarrhenae Rhizoma (Zhimu), Glycyrrhizae Radix et Rhizoma Praeparata cum Melle (Zhigancao), and nonglutinous rice (Jingmi). The chemical composition of BHT still remains largely elusive thus far. To qualitatively and quantitatively characterize secondary metabolites and carbohydrates in BHT, here a combination of analytical approaches using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and ultraperformance liquid chromatography coupled with photodiode array detector was developed and validated. A total of 42 secondary metabolites in BHT were tentatively or definitely identified, of which 10 major chemicals were quantified by the extracting ion mode of quadrupole time-of-flight mass spectrometry. Meanwhile, polysaccharides, oligosaccharides, and monosaccharides in BHT were also characterized via sample pretreatment followed by sugar composition analysis. The quantitative results indicated that the determined chemicals accounted for 35.76% of the total extract of BHT, which demonstrated that the study could be instrumental in chemical dissection and quality control of BHT. The research deliverables not only laid the root for further chemical and biological evaluation of BHT, but also provided a comprehensive analytical strategy for chemical characterization of secondary metabolites and carbohydrates in traditional Chinese medicine formulas.

    更新日期:2019-11-01
  • Morphological and chemical analyses of Eriocauli Flos sold in Taiwan markets.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    I-Jung Lee,Cheng-Pei Chung,Shu-Jen Chang,Yun-Lian Lin

    Eriocauli Flos (Gujingcao; EF), the dried capitulum with the peduncle of Eriocaulon buergerianum Koern. (Eriocaulaceae), is a Chinese herbal medicine for treating eye diseases and inflammation. However, several species of the Eriocaulon genus are used as substitutes in different areas. To examine the species of EF used in Taiwan and to establish the quality control platform, morphological and chemical analyses have been performed. Ten major compounds, including apigenin (7) and its 7-O-β-D-glucopyranoside (1) and 7-O-(6-O-E-coumaroyl)-β-D-glucopyranoside (6), hispidulin (8) and its 7-O-β-D-glucopyranoside (2) and 7-O-(6-O-E-coumaroyl)-β-D-glucopyranoside (5), jaceosidin (9) and its 7-O-β-D-glucopyranoside (3), and toralactone (10) and its 9-O-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside (4), were isolated and identified from commercially available EF. Morphological investigation showed that two kinds of EFs and most of the EFs sold in Taiwan herbal markets are capitulum without the peduncle. A simultaneous high performance liquid chromatography and ultra performance liquid chromatography analyses of multiple components (1-10) in commercially available EFs, collected from different areas of Taiwan, was conducted. Results showed wide variations in morphology and chemical profiles between capitulum with and without the peduncle. In comparison with an authentic E. buergerianum, we found not only the morphology but also the chemical profile was different from both collected samples. In terms of the morphological examination, the samples without peduncle are closer to the authentic one. To ensure the correct EF materia medica is used in Taiwan so as to guarantee their therapeutic efficacy in clinical practice, further monitoring is necessary.

    更新日期:2019-11-01
  • Red algae (Gelidium amansii) hot-water extract ameliorates lipid metabolism in hamsters fed a high-fat diet.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Tsung-Han Yang,Hsien-Tsung Yao,Meng-Tsan Chiang

    The purpose of this study was to investigate the effects of Gelidium amansii (GA) hot-water extracts (GHE) on lipid metabolism in hamsters. Six-week-old male Syrian hamsters were used as the experimental animals. Hamsters were divided into four groups: (1) control diet group (CON); (2) high-fat diet group (HF); (3) HF with GHE diet group (HF + GHE); (4) HF with probucol diet group (HF + PO). All groups were fed the experimental diets and drinking water ad libitum for 6 weeks. The results showed that GHE significantly decreased body weight, liver weight, and adipose tissue (perirenal and paraepididymal) weight. The HF diet induced an increase in plasma triacylglycerol (TG), total cholesterol (TC), low-density lipoprotein cholesterol and very-low-density lipoprotein cholesterol levels. However, GHE supplementation reversed the increase of plasma lipids caused by the HF diet. In addition, GHE increased fecal cholesterol, TG and bile acid excretion. Lower hepatic TC and TG levels were found with GHE treatment. GHE reduced hepatic sterol regulatory element-binding proteins (SREBP) including SREBP 1 and SREBP 2 protein expressions. The phosphorylation of adenosine monophosphate (AMP)-activated protein kinase (AMPK) protein expression in hamsters was decreased by the HF diet; however, GHE supplementation increased the phosphorylation of AMPK protein expression. Our results suggest that GHE may ameliorate lipid metabolism in hamsters fed a HF diet.

    更新日期:2019-11-01
  • The regulatory effects of fish oil and chitosan on hepatic lipogenic signals in high-fat diet-induced obese rats.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Chen-Yuan Chiu,Tien-Chia Chang,Shing-Hwa Liu,Meng-Tsan Chiang

    The present study investigated the regulatory effects of fish oil and chitosan on the signals of hepatic lipid metabolism and the postulated mechanism in high-fat diet-induced obese rats. Diet supplementation of chitosan and fish oil efficiently suppressed the increased weights in body and livers of high-fat diet-fed rats. Supplementation of chitosan and fish oil significantly decreased the activities of hepatic lipid biosynthesis-related enzymes and efficiently regulated plasma lipoprotein homeostasis. Both chitosan and fish oil significantly ameliorated the alterations in the protein expressions of hepatic lipogenic transcription factors (LXRα and PPARα), and could also significantly regulate the downstream hepatic lipogenic genes (FAS, HMGCR, CYP7A1, FATP, FABP, AOX, and ABCA) expressions in high-fat diet-fed rats. These results suggest that both fish oil and chitosan exerts downregulative effects on hepatic lipid metabolism in high-fat diet-induced obese rats via the LXRα inhibition and PPARα activation, which further affect the expressions of hepatic lipogenesis-associated genes.

    更新日期:2019-11-01
  • Dunaliella salina alga extract inhibits the production of interleukin-6, nitric oxide, and reactive oxygen species by regulating nuclear factor-κB/Janus kinase/signal transducer and activator of transcription in virus-infected RAW264.7 cells.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Hui-Wen Lin,Cheng-Wei Liu,Deng-Jye Yang,Ching-Chung Chen,Shih-Yin Chen,Jung-Kai Tseng,Tien-Jye Chang,Yuan-Yen Chang

    Recent investigations have demonstrated that carotenoid extract of Dunaliella salina alga (Alga) contains abundant β-carotene and has good anti-inflammatory activities. Murine macrophage (RAW264.7 cells) was used to establish as an in vitro model of pseudorabies virus-induced reactive oxygen species (ROS) response. In this study, antioxidant activities of Alga were measured based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, trolox equivalent antioxidant capacity assays, reducing power, and virus-induced ROS formation in RAW264.7 cells. Anti-inflammatory activities of Alga were assessed by its ability to inhibit the production of interleukin-6 and nitric oxide (NO) using enzyme-linked immunosorbent assay, then the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway was investigated by measuring the inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-κB (p50 and p65), JAK, STAT-1/3, and suppressor of cytokine signaling 3 (SOCS3) by Western blotting. In addition, Alga inhibited virus replication by plaque assay. Our results showed that the Alga had high antioxidant activity, significantly reduced the virus-induced accumulation of ROS, and inhibited the levels of nitric oxide and interleukin-6. Further studies revealed that Alga also downregulated the gene and protein expressions of iNOS, COX-2, nuclear factor-κB (p50 and p65), and the JAK/STAT pathway. The inhibitory effects of Alga were similar to pretreatment with specific inhibitors of JAK and STAT-3 in pseudorabies virus -infected RAW264.7 cells. Alga enhanced the expression of SOCS3 to suppress the activity of the JAK/STAT signaling pathway in pseudorabies virus-infected RAW264.7 cells. In addition, Alga has decreased viral replication (p < 0.005) at an early stage. Therefore, our results demonstrate that Alga inhibits ROS, interleukin6, and nitric oxide production via suppression of the JAK/STAT pathways and enhanced the expression of SOCS3 in virus-infected RAW264.7 cells.

    更新日期:2019-11-01
  • Antihemolytic and antioxidant properties of pearl powder against 2,2'-azobis(2-amidinopropane) dihydrochloride-induced hemolysis and oxidative damage to erythrocyte membrane lipids and proteins.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Hsin-Ling Yang,Mallikarjuna Korivi,Ming-Kuem Lin,Hebron Chun-Wei Chang,Chi-Rei Wu,Meng-Shiou Lee,William Tzu-Liang Chen,You-Cheng Hseu

    Pearl powder, a well-known traditional mineral medicine, is reported to be used for well-being and to treat several diseases from centuries in Taiwan and China. We investigated the in vitro antihemolytic and antioxidant properties of pearl powder that could protect erythrocytes against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative damage to membrane proteins/lipids. Human erythrocytes were incubated with different concentrations of pearl powder (50-200 μg/mL) for 30 minutes and then exposed to AAPH for 2-6 hours. We found that AAPH alone time dependently increased the oxidative hemolysis of erythrocytes, while pearl powder pretreatment substantially inhibited the hemolysis in a concentration-/time-dependent manner. AAPH-induced oxidative damage to erythrocyte membrane lipids was evidenced by the elevated malondialdehyde (MDA) levels. However, pearl powder remarkably inhibited the malondialdehyde formation, and the 200 μg/mL concentration showed almost similar malondialdehyde values to the control. Furthermore, pearl powder suppressed the AAPH-induced high-molecular-weight protein formation and concomitantly increased the low-molecular-weight proteins in erythrocytes. Antioxidant potential that was measured as superoxide dismutase activity and glutathione content was significantly dropped by AAPH incubation, which suggests the vulnerability of erythrocytes to AAPH-induced oxidative stress. Noteworthy, erythrocytes pretreated with pearl powder showed restored superoxide dismutase activity and glutathione levels against AAPH-induced loss. Our findings conclude that pearl powder attenuate free radical-induced hemolysis and oxidative damage to erythrocyte membrane lipids/proteins. The potent antioxidant property of pearl powder may offer protection from free radical-related diseases.

    更新日期:2019-11-01
  • The impact of gallic acid on the methotrexate-induced kidney damage in rats.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Halil Asci,Ozlem Ozmen,Hamit Yasar Ellidag,Bunyamin Aydin,Ercan Bas,Necat Yilmaz

    Prolonged use of an antineoplastic agent methotrexate (MTX), can cause numerous side effects such as nephrotoxicity. The aim of this study was to examine the effects of MTX on kidneys and demonstrate the protective effects of gallic acid (GA). Twenty-four, male, rats distributed into three groups. Each groups consisted eight rats and only saline was administered to the control group. The MTX group received a single dose (20 mg/kg) MTX intraperitoneally. The MTX + GA group received same dose MTX and 100 mg/kg GA orally during the 7 days. Renal functions, oxidative stress markers, histopathological and immunohistochemical changes were evaluated at the end of the experiment. Blood urea nitrogen, creatinine, uric acid levels and tissue oxidative stress markers, total oxidant status and oxidative stress index levels significantly increased and total antioxidant status levels significantly decreased in MTX group compared with the control group. At the histopathological examination hemorrhages, tubular cell necrosis, glomerulosclerosis, inflammatory cell infiltrations and proteinous materials in tubules were noticed in MTX group. Immunohistochemical examination revealed that increased expressions of serum amyloid A (SAA), tumor necrosis factor alpha (TNF-α), prostaglandin E2 (PGE-2) and C-reactive protein (CRP) in tubular epithelial cells of kidneys in this group. There were no immunoreaction with SAA and CRP, only small number of PGE-2 and TNF-α positive tubular epithelial cells were observed in MTX + GA group. In conclusion, all evidence suggested that oxidative stress caused MTX-induced nephrotoxicity and GA prevent the kidney from the nephrotoxicity due to its antioxidant and anti-inflammatory activities.

    更新日期:2019-11-01
  • Anti-oxidant activity and major chemical component analyses of twenty-six commercially available essential oils.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Hsiao-Fen Wang,Kuang-Hway Yih,Chao-Hsun Yang,Keh-Feng Huang

    This study analyzed 26 commercially available essential oils and their major chemical components to determine their antioxidant activity levels by measuring their total phenolic content (TPC), reducing power (RP), β-carotene bleaching (BCB) activity, trolox equivalent antioxidant capacity (TEAC), and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging (DFRS) ability. The clove bud and thyme borneol essential oils had the highest RP, BCB activity levels, and TPC values among the 26 commercial essential oils. Furthermore, of the 26 essential oils, the clove bud and ylang ylang complete essential oils had the highest TEAC values, and the clove bud and jasmine absolute essential oils had the highest DFRS ability. At a concentration of 2.5 mg/mL, the clove bud and thyme borneol essential oils had RP and BCB activity levels of 94.56% ± 0.06% and 24.64% ± 0.03% and 94.58% ± 0.01% and 89.33% ± 0.09%, respectively. At a concentration of 1 mg/mL, the clove bud and thyme borneol essential oils showed TPC values of 220.00 ± 0.01 and 69.05 ± 0.01 mg/g relative to gallic acid equivalents, respectively, and the clove bud and ylang ylang complete essential oils had TEAC values of 809.00 ± 0.01 and 432.33 ± 0.01 μM, respectively. The clove bud and jasmine absolute essential oils showed DFRS abilities of 94.13% ± 0.01% and 78.62% ± 0.01%, respectively. Phenolic compounds of the clove bud, thyme borneol and jasmine absolute essential oils were eugenol (76.08%), thymol (14.36%) and carvacrol (12.33%), and eugenol (0.87%), respectively. The phenolic compounds in essential oils were positively correlated with the RP, BCB activity, TPC, TEAC, and DFRS ability.

    更新日期:2019-11-01
  • Taiwanese and Japanese yam (Dioscorea spp.) extracts attenuate doxorubicin-induced cardiotoxicity in mice.
    J. Food Drug Anal. (IF 4.176) Pub Date : 2017-10-11
    Chih-Tai Chen,Zhi-Hong Wang,Cheng-Chin Hsu,Hui-Hsuan Lin,Jing-Hsien Chen

    The present study was designed to explore whether yam could protect the heart from doxorubicin (DOX)-induced oxidative stress leading to cardiotoxicity in vivo. In this study, the protective effects of water and ethanol extracts of three varieties of yam, including water extracts of Dioscorea japonica Thunb., ethanol extracts of D. japonica Thunb., water extracts of Dioscorea alata, ethanol extracts of D. alata, water extracts of Dioscorea purpurea, and ethanol extracts of D. purpurea, against DOX-induced cardiotoxicity in experimental mice were evaluated. DOX treatment led to significant decreases in the ratio of heart weight to body weight and heart rate, and increases in blood pressure and the serum level of lactate dehydrogenase, a marker of cardiotoxicity, were recovered by yam extracts, especially in water extracts of D. alata. Yam extracts also decreased the cardiac levels of thiobarbituric acid relative substances, reactive oxygen species, and inflammatory factors, as well as the expression of nuclear factor kappa B, while ethanol extracts of D. japonica Thunb. and D. purpurea were shown to be more potent. Moreover, yam extracts had a role in increasing the activities of glutathione peroxidase and superoxide dismutase, thus improving the DOX-induced alterations in oxidative status in the heart tissue of DOX-treated mice. All ethanol extracts of yam exhibited their antiapoptotic abilities on caspase-3 activation and mitochondrial dysfunction, and ethanol extracts of D. alata still exerted a superior effect. Based on these findings, it can be concluded that yam has significant cardioprotective properties against DOX-induced damage via its multiple effects on antioxidant, anti-inflammatory, or antiapoptotic activities.

    更新日期:2019-11-01
Contents have been reproduced by permission of the publishers.
导出
全部期刊列表>>
2020新春特辑
限时免费阅读临床医学内容
ACS材料视界
科学报告最新纳米科学与技术研究
清华大学化学系段昊泓
自然科研论文编辑服务
加州大学洛杉矶分校
上海纽约大学William Glover
南开大学化学院周其林
课题组网站
X-MOL
北京大学分子工程苏南研究院
华东师范大学分子机器及功能材料
中山大学化学工程与技术学院
试剂库存
天合科研
down
wechat
bug