-
Structural and Compositional Changes in Two Marine Shell Traditional Chinese Medicines: A Comparative Analysis Pre- and Post-Calcination J. AOAC Int. (IF 1.6) Pub Date : 2024-03-16 Lizhu Wu, Chenlu Liu, Tao Yao, Yun Shi, Jinyang Shen, Xun Gao, Qin Kunming
Background Arcae concha and Meretricis concha cyclinae concha are two marine shellfish herbs with similar composition and efficacy, which are usually calcined and used clinically. Objective This study investigated variations in the inorganic and organic components of Arcae concha and Meretricis concha cyclinae concha from different production regions, both Arcae concha and Meretricis concha cyclinae
-
Purity Assessment of Honey Based on Compound Specific Stable Carbon Isotope Ratios Obtained by LC-IRMS J. AOAC Int. (IF 1.6) Pub Date : 2024-03-15 Franz Ulberth, Eric Aries, Oliver De Rudder, Georgios Kaklamanos, Alain Maquet
Background The use of stable carbon isotope ratios (δ13C) of sugar fractions of honey is a powerful tool to detect adulteration with sugar syrups. This is accomplished by calculating differences of the δ13C values between individual honey saccharides and comparing them to published purity criteria. A liquid chromatography—isotope ratio mass spectrometry (LC-IRMS) method for the determination of δ13C
-
A Novel Approach for Single Step Analyte Fractionation of Raw Milk Prior to Antbiotica Residue Trace Analysis as an Alternative to QuEchERS Based Extraction J. AOAC Int. (IF 1.6) Pub Date : 2024-03-10 Jan-Michael Steils, Maren Lang, Melina Kraus, Klaus Schöne, John Cashman, Christian Baumgartner
Background Antibiotic residues in milk are a well-known hazard in the dairy food chain. Detection methods for these residues, such as non-specific microbiological inhibitor tests or group-specific receptor tests, respectively, are relatively inexpensive, easy to use and widely applied to ensure food safety. In contrast, specific detection by liquid chromatography tandem mass spectrometry (LC-MS/MS)
-
Ecofriendly Simultaneous Estimation of Ponceau 4R and Carmoisine Employing Analytical Quality by Design Aided RP-HPLC Method in Commercial Food Samples Utilizing Green Ultrasound Assisted Extraction Technique J. AOAC Int. (IF 1.6) Pub Date : 2024-03-08 Atyurmila Chakraborty, Kavitha Jayaseelan
Background Ponceau 4R (E124) and Carmoisine (E122) are frequently utilized azo synthetic dye in the food industry owing to their aesthetically pleasing coloration and broad consumer acceptability. It is imperative to prioritize environmentally favorable technologies for quantifying these dyes, as excessive consumption of those poses significant health risks. Objective The primary objective of this
-
Development of a Mass Spectrometry-Based Method for Quantification of Total Cashew Protein in Roasting Oil J. AOAC Int. (IF 1.6) Pub Date : 2024-03-02 Shimin Chen, Melanie L Downs
Background Food allergen cross-contact during food preparation and production is one of the causes of unintentional allergen presence in packaged foods. However, little is known about allergen cross-contact in shared frying or roasting oil, which prevents the establishment of effective allergen controls and may put allergic individuals at risk. To better understand the quantity of allergen transferred
-
Identification, Isolation, and Charecterisation of a Novel Degradation Impurity of Busulfan Using Preparative Chromatography, NMR and LC-MS J. AOAC Int. (IF 1.6) Pub Date : 2024-03-02 Rajender Reddy Yerla, Surendra Babu Manubolu Surya
Background Busulfan is the most effective medication for treating chronic myelogenous or granulocytic leukemia because it has cytotoxic properties that harm or kill hematopoietic cells. It cannot absorb light in the ultraviolet range due to its structure. Because of this, it is very challenging to quantify using traditional HPLC coupled with UV/PDA detectors. So, using sodium diethyldithiocarbamate
-
Analysis of Secondary Metabolites of Elaeagnus Angustifolia Leaves Based on UPLC-Q-TOF-MS J. AOAC Int. (IF 1.6) Pub Date : 2024-02-29 Jinfa Liao, Liyan Liu, Lu Yang, Wei Sheng, Ke Zhang, Bin Zhou, Silin Yu, Yongzhi Yin, Jinhui Wang
Background The leaves of Elaeagnus angustifolia, belonging to the Elaeagnaceae Juss. family, are known for their medicinal properties for relieving cough and asthma, as well as treating dysentery and diarrhea. Objective To establish a rapid qualitative method for the detection of secondary metabolites in leaves of Elaeagnus angustifolia, including the identification and analysis of various secondary
-
Different Eco-Friendly Spectrophotometric Approaches Including Direct and Manipulating Zero and Ratio Spectra for Simultaneous Determination of Novel Nasal Spray Combination Used in Seasonal Allergic Rhinitis J. AOAC Int. (IF 1.6) Pub Date : 2024-02-27 Alaa Reda, Hanaa Saleh, Eman A Bahgat, Michael Gamal Fawzy
Background The presentation of rhinitis has recently drawn increasing attention in recent years due to the possibility of overlap or confusion between allergic rhinitis symptoms and those of pandemic COVID-19. Azelastine hydrochloride (AZH) and mometasone furoate (MOF) are two of the most efficient combinations for enhancing the symptoms of seasonal allergic rhinitis. Objective This work concerns with
-
Complexity of Translating Analytics to Recent Cannabis Use and Impairment J. AOAC Int. (IF 1.6) Pub Date : 2024-02-27 Michael W Degregorio, Chiao-Jung Kao, Gregory T Wurz
While current analytical methodologies can readily identify cannabis use, definitively establishing recent use within the impairment window has proven to be far more complex, requiring a new approach. Recent studies have shown no direct relationship between impairment and Δ9-tetra-hydrocannabinol (Δ9-THC) concentrations in blood or saliva, making legal “per se” Δ9-THC limits scientifically unjustified
-
Analytical Method for Quantifying Monoterpenoids (p-Cymene and Myrcene) in Nanoemulsions Using High-Performance Liquid Chromatography J. AOAC Int. (IF 1.6) Pub Date : 2024-02-24 Jonatas Lobato Duarte, Leonardo Delello Di Filippo, Alberto Gomes Tavares Junior, Marlus Chorilli
Background Myrcene and cymene, aromatic monoterpenes found in plants and essential oils, possess distinctive aromatic qualities. However, their volatility and limited solubility pose challenges in precise handling and formulation. Meanwhile, nanoemulsions emerge as promising drug delivery systems, improving the bioavailability and stability of these active ingredients. Objective This article aimed
-
Effect of selective enrichment storage temperature and duration time on the detection of Salmonella in food J. AOAC Int. (IF 1.6) Pub Date : 2024-02-23 Lijun Hu, Guodong Zhang
Background For pathogen detection in food, there are occasions where samples cannot be processed immediately after selective enrichment or need to be reexamined days/weeks later for confirmation or retest. Objective This study aimed to investigate the effect of different prolonged period of storage of selective enrichments of food at 4 ± 2 °C and room temperature (20—22 °C) on the detection and isolation
-
A Robust HPLC Approach for Quantitation of Camptothecin in Mesoporous Silica Nanoparticles Matrix and in the Presence of Its Degradation Products J. AOAC Int. (IF 1.6) Pub Date : 2024-02-23 Richa Dave, Neel Savaliya, Ashish Dobariya, Rashmin Patel, Mrunali Patel
Background Camptothecin is a potent anti-cancer drug used for the treatment of various cancers. Objective The goal of this research investigation was to develop and validate a new stability indicating HPLC technique for the quantitative assessment of camptothecin in in-house developed mesoporous silica nanoparticles, a novel nanoformulation matrix for the treatment of cancer. Method The Waters Inertsil®
-
AMR Threat Perception Assessment of Heterotrophic Bacteria from Shrimp Aquaculture through Epidemiological Cut off Values J. AOAC Int. (IF 1.6) Pub Date : 2024-02-17 Ranjit Kumar Nadella, Satyen Kumar Panda, Kumar Anuj, Devananda Uchoi, Pankaj Kishore, Madhusudana Rao Badireddy, Pani Prasad Kuricheti, Ram Prakash Raman, Mukteswar Mothadaka Prasad
Background Emergence and dissemination of antibiotic resistance is one of the major risks associated with the rampant usage of antibiotics in food-producing animals including aquaculture. Objective To determine Epidemiological Cut-OFF (ECOFF) values of heterotrophic bacterial populations from shrimp culture environment against five different antibiotics Methods In this present study, 2304 heterotrophic
-
Eco-Friendly Stability-Indicating HPLC Method for Related Compounds in Pemetrexed Ditromethamine (Antineoplastic Agent) for Injection J. AOAC Int. (IF 1.6) Pub Date : 2024-02-04 Arjuna Rao Nekkalapudi, Srinivasu Navuluri, Sreenivas Pippalla
Background The Eco-friendly analytical technique was developed for intention of preserving the environment by using green chemistry principles. Pemetrexed is folate analogue indicated for treatment of advanced lung cancer. Objective Development of Green Stability indicating HPLC method for quantification of Pemetrexed (Ditromethamine) impurities in API and parenteral dosage form. Method Chromatographic
-
Molecular Interactions Leading to the Advancement in the Techniques for COVID-19 Detection: A Review J. AOAC Int. (IF 1.6) Pub Date : 2024-02-04 Mohammad Kashif, Swati Acharya, Adila Khalil
Since 2019 the world is in a combat with the highly contagious disease COVID-19 that is caused by the rapid transmission of SARS-CoV-2 virus (severe acute respiratory syndrome- coronavirus-2). Detection of this disease in an early stage helps to control its spread and management. To combat this epidemic with one-time effective medication improved quick analytical procedures must be developed and validated
-
QbD Based Stability Indicating RP-HPLC Method Development and Validation for the Estimation of Favipiravir—an Eco-Friendly Approach J. AOAC Int. (IF 1.6) Pub Date : 2024-02-02 M Siri Chandana, K Sujatha, A Ajitha, P Narendra, K Sonia
Background Analytical quality by design (AQbD) affords a systematic scaffolding to triumph a continuously validated, robust assay as well as life cycle management. The resuscitative repurposed drug Favipiravir, an oral drug approved for reemerging pandemic influenza in Japan - 2014 used for the treatment of life threatening pathogens such as Ebola, Lassa virus and currently COVID-19. Favipiravir is
-
Identification and Characterization of the Chemical Constituents of Qianlie Shule Capsules by UPLC-Q-Orbitrap-MS/MS J. AOAC Int. (IF 1.6) Pub Date : 2024-02-02 Huimin Liu, Nie jiaxuan, Yue Zhang, Jiayi Wang, Lili Song, yubo Li
Background Qianlie Shule capsules is a classical Chinese medicine compound preparation that is frequently used in therapeutic settings to alleviate astringent pain in the urethra, prostatic hypertrophy, and chronic prostatitis or urinary frequency. However, a comprehensive analysis of the chemical composition of Qianlie Shule capsules has not been reported. Objective To establish a quick and effective
-
Determination of Acrylamide in Coffee, Cereals, Baby Food, Cocoa, Dry Petfood, Potato Products, Vegetable Crisps, Biscuit, Tea, Nuts and Spices by LC-MS/MS in a Single Laboratory Validation: First Action 2023.01 J. AOAC Int. (IF 1.6) Pub Date : 2024-01-26 Aude Bebius, Frédérique Reding, Viviane Theurillat, Leloup Valérie, Erik Konings, Thierry Delatour, Aurélien Desmarchelier
Background Acrylamide (AA) is a process contaminant naturally formed during the cooking of starchy food at high temperatures. Considering existing risks of misquantification inherent to the analysis of AA, an AOAC initiative raised the need for a consensus standard to determine AA in a broad variety of food. Objective A quantitative LC-MS/MS method for AA determination in food was validated in a single
-
Comparison of 6 aptamer-aptamer pairs on rapid detection of SARS-CoV-2 by lateral flow assay J. AOAC Int. (IF 1.6) Pub Date : 2024-01-11 Dilek Çam Derin, Enes Gültekin, Elif Gündüz, Barış Otlu
Background SARS-CoV-2 is a threat for humanity. Both the Spike (S) protein and its receptor binding domain (sRBD) are extensively used for the rapid detection. Although real time reverse transcription polymerase chain reaction (rRT-PCR) is mostly used method for the molecular detection of SARS-CoV-2, rapid assays for antigenic detection is always needed. Lateral flow assays (LFAs) are the most commonly
-
Relevance of Secondary Enrichment in the Detection of Salmonella Spp in Food Samples by qPCR according to DIN 10135 J. AOAC Int. (IF 1.6) Pub Date : 2024-01-10 F Codony, G Agustí, L Barreto, D Asensio
Background When detecting Salmonella spp. in food samples, unlike with culture-based procedures where there are solid standards, PCR techniques are generally dominated by commercial solutions, often backed by the conformity of reference organizations, and based on rigorous validation studies. The few independent standards that exist are not subject to revision and improvement to the same extent as
-
Stability Study Through Water Activity Measurements for Dispensed Powdered Raw Materials J. AOAC Int. (IF 1.6) Pub Date : 2024-01-09 Harold A Prada-Ramírez, Juan Pablo Montes-Tamara, Eduardo A Rico-Jimenez, Juan Camilo Fonseca
Background Holding times for raw materials are relevant, since they enable us to understand the allowable time that a raw material can be kept under ideal storage conditions before the start of the manufacturing process without its quality attributes being affected. The quantification of water activity can be used as an indicator of the microbiological, physicochemical, and organoleptic stability of
-
Determination of Seven Human Milk Oligosaccharides (HMO) in Infant Formula and Adult Nutritionals: First Action 2022.07 J. AOAC Int. (IF 1.6) Pub Date : 2024-01-09 Thierry Bénet, Nathalie Frei, Véronique Spichtig, Denis Cuany, Sean Austin
Background Human milk oligosaccharides (HMOs) are important components of breast milk and may be responsible for some of the benefits of breastfeeding, including resistance to infections and the development of a healthy gut microbiota. Selected HMOs are now available for addition to infant formula, and suitable methods to control the dosing rate are needed. Objective To develop and validate a suitable
-
Variation in Botanical Reference Materials: Similarity of Actaea racemosa Analyzed by Flow Injection Mass Spectrometry J. AOAC Int. (IF 1.6) Pub Date : 2023-12-23 James Harnly, Roy Upton
Background Botanical reference materials (BRMs) generally account for the species, cultivar, and year and location of harvest that result in variability in the chemical composition that may lead to statistically significant differences using chemometric methods. Objective To Compare the chemical composition of 5 species of Actaea root BRMs, 4 herbal sources of A. racemosa root BRMs, and A. racemosa
-
Sugar Profile Method by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection in Food, Dietary Supplements, Pet Food, and Animal Feeds: Interlaboratory Validation Study, Final Action 2018.16 J. AOAC Int. (IF 1.6) Pub Date : 2023-12-23 Andrew J Ruosch, David J Ellingson
Background A method for sugar profile analysis granted First Action 2018.16 was subjected to a multi-laboratory study. Objective Perform a multi-laboratory study with this method to determine the performance parameters of repeatability and reproducibility against the AOAC Standard Method Performance Requirements (AOAC SMPR 2018.001) for Final Action status. Methods Eleven laboratories from three different
-
Simultaneous Uplc Assay for Oxitropium Bromide and Formoterol Fumarate Dihydrate in Pressurised Metered Dose Inhaler Products for Chronic Obstructive Pulmonary Disease J. AOAC Int. (IF 1.6) Pub Date : 2023-12-18 Serdar Seckin, Serap Saglik Aslan
Backround Oxitropium Bromide (OB) and Formoterol Fumarate Dihydrate (FFD) are inhaler molecules that are widely used in the treatment of chronic lung diseases. Objective The goal of this work was to create a reversed phase-ultra performance liquid chromatography (RP-UPLC) technique for assay and identification of OB and FFD, as well as identification and estimate of its associated compounds in pressurised
-
Greenness assessment and validation of HPLC method for simultaneous determination of Resveratrol and Vitamin E in dietary supplements J. AOAC Int. (IF 1.6) Pub Date : 2023-12-14 Marina Topkoska, Martina Miloshevska, Marjan Piponski, Irena Slaveska Spirevska, Natalija Nakov, Katerina Brezovska, Jelena Acevska
Background There is an increasing interest in the use of a combination of trans-resveratrol and vitamin E in dietary supplements. Determination of the content of both components is essential for confirmation of the quality of the product. Objective To establish the applicability and ensure the greenness of the previously developed high-throughput HPLC/UV method for the simultaneous determination of
-
Quality evaluation of gentamicin sulfate reference standards in Japanese Pharmacopoeia using hydrophilic interaction chromatography combined with tandem mass spectrometry J. AOAC Int. (IF 1.6) Pub Date : 2023-12-09 Keiko Maekawa, Ryuichi Sawa, Mari Matsui, Toshifumi Konda, Yumiko Kubota, Ayaka Matsuo, Akiho Maeda, Chisato Takahashi, Tsuyoshi Tanimoto, Yukari Nakagawa, Sachiyo Yoneda, Yuri Mori, Satowa Suzuki
Background Through the recent development of analytical technology, antibiotics quantification in the Japanese Pharmacopoeia (JP) has changed from traditional microbiological assays to physicochemical methods with high specificity and precision. However, for several multicomponent antibiotics without typical UV absorption, potency cannot be directly determined using instrumental methods such as high-performance
-
Exciting Advances in Sustainable Spectrophotometric Micro-Quantitation of an Innovative Painkiller “Tramadol and Celecoxib” Mixture in the Presence of Toxic Impurity, Promoting Greenness and Whiteness Studies J. AOAC Int. (IF 1.6) Pub Date : 2023-12-09 Eman A Bahgat, Hisham Hashem, Hanaa Saleh, Ebraam B Kamel, Maya S Eissa
Background Tramadol (TRM) and celecoxib (CLX) are a novel mixture that helps relieve impetuous, acute pain when other painkillers have no action. It is also reported that the currently studied drugs, tramadol and celecoxib, are used to control COVID-19 symptoms. Objective The current work highlights three important pillars of modern pharmaceutical analysis, which are as follows; impurity profiling
-
Validation of the FSTestTM Aerobic Count Plates Method for Enumeration of Aerobic Bacteria in a Variety of Matrixes and Stainless Steel Environmental Surface: AOAC Performance Tested MethodSM 112301 J. AOAC Int. (IF 1.6) Pub Date : 2023-12-09 Yun Liu, Jun Fan, Xia Sun, Zican Zhou
Background The FSTestTM Aerobic Count (AC) Plates are ready-to-use culture mediums containing nutrients, a cold-water-soluble gelling agent and a chromogenic indicator. Objective The objective of this study was to validate the FSTest AC plate method for the AOAC Performance Tested MethodsSM (PTM) certification for a variety of foods and stainless steel. Methods The performance of the FSTest AC plates
-
Simultaneous quantification of Two Neonicotinoids using QuERChERS-LC-MS/MS in Moroccan Spearmint (Mentha Spicata.L): Qualimetry of the method by Uncertainty estimation using Generalized Pivotal Quantities approach and Monte Carlo simulation J. AOAC Int. (IF 1.6) Pub Date : 2023-12-08 Hicham Aaziz, Taoufiq Saffaj, Yassine Hameda Benchekroun, Bouchaib Ihssane
Background Neonicotinoids are used for the phystosanitary treatment of Mentha Spicata.L crops, and this practice requires precise control of these harmful substances at very low concentrations. Objective The objective of this study is to apply an approach allowing simultaneously validation and evaluation of measurement uncertainty based on total error methodology, in order to accurately quantify the
-
Chloroplast genome sequences and phylogenetic analysis of eight newly sequenced Caryophyllaceae species J. AOAC Int. (IF 1.6) Pub Date : 2023-12-01 Rongpeng Liu, Zejing Mu, Xiaolang Du, Guoyue Zhong, Xiaoyun Wang
Background Caryophyllaceae is a big family composed of many economic and medicinal species. However, the phylogeny of the family is insufficient and genome data are lacking for many species. Objective Using the next-generation sequencing (NGS) to acquire the chloroplast (cp) genomes of Eremogone acicularis (F.N.Williams) Ikonn., E. brevipetala (Tsui & L.H.Zhou) Sadeghian & Zarre, E. bryophylla (Fernald)
-
Utility of N-Bromosuccinimide–Water Combination as a Green Reagents for A validated Indirect Spectrophotometric Determination of Some Antihypertensive Drugs: An Application to Their Monitoring in Marketed Tablets and Capsules J. AOAC Int. (IF 1.6) Pub Date : 2023-12-01 Marwa I Helmy, Christine K Nessim
Background Analytical test was conducted to investigate the use of N-bromosuccinimide (NBS) as an important safe analytical reagent for the spectrophotometric detection of therapeutically significant dihydropyridine-based calcium antagonists (DHP) namely nifedipine (NIF) and amlodipine (AML) that have been demonstrated to possess antioxidant activity in vivo and to reduce the intracellular production
-
A Single-Laboratory Validation of a UPLC Method for Determination of Folic Acid in Various Dietary Supplement Dosage Forms J. AOAC Int. (IF 1.6) Pub Date : 2023-11-30 Lusi A, Mina Fakhary, Niloufar Rahimi Gaeini, Jennifer M Solano, Mohamed Koroma
Background Folic acid is an essential nutrient necessary for the synthesis of nucleic acids (DNA and RNA) and certain amino acids. There are no scientifically validated analytical methods for folic acid applicable to all dosage forms. Objective A single-laboratory method was validated for the determination of folic acid content in various dietary supplement dosage forms. This method used ultra-performance
-
Simultaneous Determination of Amphenicols in Animal-Derived Foods by Solvent and Solid Phase Extraction with Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry J. AOAC Int. (IF 1.6) Pub Date : 2023-11-29 Feng Liu, Yaya Yan, Yao Yi, Yingxu Qin, Fei Xu
Background The consumption of foods containing amphenicols, a type of antibiotic, is a major concern for human health. A stable and accurate detection method can provide technical support for the food-safety monitoring. Objective An effective and efficient method was established for determining amphenicols in animal-derived foods through the simultaneous use of solid-phase extraction (SPE) cleanup
-
Bitter-pungent flavor identification based on ingredient information similarity of Chinese herbal medicines J. AOAC Int. (IF 1.6) Pub Date : 2023-11-12 Guohui Wei, Min Qiu, Chune Li, Xiaoyan Wang, Xianjun Fu
Background The flavor theory of Chinese herbal medicines (CHMs) is one of the core theories of traditional Chinese medicine (TCM). Accurate flavor identification of CHMs is essential to guide the clinical application of CHMs. Objective To develop a new method for flavor identification of CHMs according to ingredient information of CHMs. Methods It was found that the chemical basis of medicinal flavors
-
Reverse flow injection spectrophotometric determination of total acidity in beverages using butterfly pea flower extract J. AOAC Int. (IF 1.6) Pub Date : 2023-11-10 Patpitcha Deecharoenchaiyakul, Napa Tangtreamjitmun
Background Monitoring total acidity during beverage production is crucial for quality control. The standard acid-base titration, though widely used, is slow and generates hazardous waste through the use of acid-base indicators. Objective To develop an analysis method for beverage samples to determine total acidity using a natural reagent from butterfly pea flower as the colorimetric reagent. Methods
-
Assessment of the Water Sorption Capacity of Rifaximin Using the Dynamic Vapor Sorption Technique for Optimization of the Choice of Excipients and the Manufacturing Environment of Rifaximin Tablets. J. AOAC Int. (IF 1.6) Pub Date : 2023-11-02 Naga Venkata Durga Prasad Ketha,Hanimi Reddy Bapatu,Kumaraswamy Ummiti,Praveen Kumar Subbappa,Deepti Kolli
BACKGROUND Rifaximin, a medication of the rifamycin family with two distinct strengths of 200 mg and 550 mg in tablet form, is useful for the treatment of travelers' diarrhea. It has a solid yellow hue and is very hygroscopic in nature. It exhibits a variety of polymorphic forms such as α, β, γ, δ, and ε depending on bonded moisture. These polymorphs' varying chemical and physical characteristics,
-
Journal of AOAC INTERNATIONAL: A History of Continued Value and Relevance. J. AOAC Int. (IF 1.6) Pub Date : 2023-11-02 Xu-Liang Cao
-
Developed and Validated for the estimation of Tapinarof in Topical Formulation and Active Pharmaceutical Ingredients J. AOAC Int. (IF 1.6) Pub Date : 2023-10-13 Raghunatha Reddy C, Nageswara Reddy Gosu
Background Its broad applicability and capacity to separate numerous components in a single chromatographic run led to the initial recognition of RP-HPLC as an analytical technique. Objective The objective of this study was to create a straightforward and reliable method for accurately and precisely measuring the amount of Tapinarof in both the topical formulation and the active pharmaceutical ingredient
-
Innovative UV protocols based on straightforward mathematical filtration for concurrent estimation of two antidiabetic drugs in their brand-new combination: A comparative study J. AOAC Int. (IF 1.6) Pub Date : 2023-10-12 Israa M Nour, Ahmed R Mohamed, Mohamed Badrawy
Background In 2019, the FDA approved a brand-new combination of linagliptin and empagliflozin in a formulation called Glyxambi® tablets for managing type-2 diabetes mellitus. Nowadays, spectrophotometric techniques occupy the first place among their peers in terms of ease of application, friendliness to the environment, and low costs. Objective This research discusses the development of two very simple
-
Determination of cannabinoids in Cannabis sativa oil and infused ice cream by LC-DAD method J. AOAC Int. (IF 1.6) Pub Date : 2023-10-11 Jefree J Raslan-Jaramillo, Gisela A Ríos-Gajardo, Marcia A Avello, Marta G de Diego
Background Cannabis sativa is known to produce a class of terpenophenolic compounds named cannabinoids. The two main ones are cannabidiol (CBD) and tetrahydrocannabinol (THC), which have therapeutic properties. In the development of cannabis-based preparations it is important to have suitable analytical methods for the analysis of the principal cannabinoids. Objective This study aimed to develop and
-
Determination of Lactose in Lactose-free and Low-lactose Milk, Milk Products, and Products Containing Dairy Ingredients by the LactoSens®R Amperometry Method: Final Action 2020.01 J. AOAC Int. (IF 1.6) Pub Date : 2023-10-09 Elisabeth Halbmayr-Jech, Roman Kittl, Patrick Weinmann, Christopher Schulz, Anna Kowalik, Matthias König, Jasmin Korp, Christoph Sygmund, Sharon L Brunelle
Background The LactoSens®R method was previously shown to have acceptable accuracy and repeatability precision as required by AOAC Standard Method Performance Requirements (SMPR®) 2018.009 for determination of lactose in low-lactose or lactose-free milk, milk products, and products containing dairy ingredients and was awarded Official Method of AnalysisSM (OMA) First Action status in 2020. Objective
-
Using Liquid Chromatography—Isotope Ratio Mass Spectrometry for Detection of Economically Motivated Adulteration of Maple Syrup J. AOAC Int. (IF 1.6) Pub Date : 2023-09-29 Madhavi Mantha, Kevin M Kubachka, John R Urban, Jana L Brueggemeyer, Lisa A Kaine, Reagan A Patton
Background Maple syrup is a sought-after commodity and an active target of economically motivated adulteration (EMA), similar to other foods such as lemon juice and honey. It is used as a condiment and a sweetener. Objective This study is aimed to detect adulteration in maple syrup via an internal standard method using malic acid through solid phase extraction (SPE) and liquid chromatography with isotope
-
Determination of Tilmicosin in Bovine, Swine, Chicken, and Turkey Tissues by Liquid Chromatography with Tandem Mass Spectrometry, Single Laboratory Validation J. AOAC Int. (IF 1.6) Pub Date : 2023-09-29 John Matt Rodewald, Thomas J Burnett, Sharon L Brunelle, Clive Ward, Mark R Coleman
Background An LC-MS/MS method was developed for determination and confirmation of tilmicosin in bovine, swine, chicken, and Turkey tissues (liver, kidney, muscle, and skin/fat) and bovine milk. Objective The method was subjected to single laboratory validation to establish method performance parameters. Methods Animal tissues and bovine milk were fortified at 4 concentrations ranging from 0.5 times
-
Quantification of Drospirenone and Ethinyl Estradiol Related Impurities in a Combined Pharmaceutical Dosage Form by Chromatography Method with QbD Robustness Study J. AOAC Int. (IF 1.6) Pub Date : 2023-09-28 Srinivasa Reddy Chinta, Vaishnavi Chintala, Vishnu Nandimalla, Rajyalakshmi Ch, Sasikiran Goud Ediga, Leela Prasad Kowtharapu, Naresh Kumar Katari
Background The estimation of drugs (contain Drospirenone (DRSP) and Ethinyl Estradiol (EE)) and their related impurities in low-dose oral contraceptive drug products is an extremely challenging target. The proposed research sought to develop and validate a stability-indicating method for quantifying drug substances and its related impurities in tablet formulation. Objective To develop and validate
-
GC-MS analysis of primary aromatic amines originated from azo dyes in commercial textile or leather products using helium alternative carrier gases J. AOAC Int. (IF 1.6) Pub Date : 2023-09-27 Maiko Tahara, Tsuyoshi Kawakami, Yoshiaki Ikarashi
Background In recent years, due to the global shortage of helium gas, the development of gas chromatography analytical methods using alternative to helium carrier gases is necessary. Objective The objective of this study was to examine the applicability of hydrogen and nitrogen as alternative carrier gases using the test method for azo compounds in the Act on Control of Household Products Containing
-
Influence of organic and inorganic compounds of various metals on the synthesis of polysaccharides by the medicinal mushroom Trametes versicolor J. AOAC Int. (IF 1.6) Pub Date : 2023-09-27 Kairat Mustafin, Zhanara Suleimenova, Zhanar Narmuratova, Nurlan Akhmetsadykov, Aigul Kalieva
Background To date, basidiomycetes are considered as promising objects of biotechnology, due to a number of biologically active compounds, such as polysaccharides and triterpenes. These compounds have a high therapeutic potential and demonstrate immunomodulatory, antiviral, and antifungal activities. Objective The purpose of this study was to study the effect of various concentrations of metal citrates
-
Quantification of Total Sulfite in Shrimps by BIOFISH 300 SUL Method: First Action 2021.09 J. AOAC Int. (IF 1.6) Pub Date : 2023-09-26 Jone Garate, Arrate Jaureguibeitia, Roberto Gonzalez, Itziar Ortiz de Zarate, Sandra Salleres
Background Sulfite is the oldest and most widely used additive in our food supply with antioxidant and preservative properties. Due to its allergenic-like reactions and other adverse health effects, it`s use is regulated by international regulatory bodies. Therefore, food industries as well as regulatory laboratories must ensure that the maximum concentration of sulfite permitted is not exceeded. The
-
Optimization of a solid phase extraction coupled with high performance liquid chromatography and diode array ultraviolet detection method for monitoring of different antibiotic classes residues in water samples J. AOAC Int. (IF 1.6) Pub Date : 2023-09-23 Rania A Mahmoud, Ghada M Hadad, Randa A Abdel Salam, Hatem I Mokhtar
Background The increased use of cephalosporin antibiotics in the last few years as well as the detection of their residues in wastewater treatment plants and hospital wastewater posed a risk for their residue’s infiltration to environmental water samples. Objective A simplified, sensitive, and convenient solid phase extraction (SPE) procedure coupled with either HPLC or fast HPLC methods with diode
-
Validation of PVT VIABLE® for Detection of Legionella in Potable and Non-potable Water: AOAC Performance Tested MethodSM 082303 J. AOAC Int. (IF 1.6) Pub Date : 2023-09-23 Leah Wickenberg, Katherine Gabrie, Patrick McCarthy, Melissa Cain
Background PVT (Phigenics Validation Test) VIABLE® (Viability Identification Assay by Legionella Enrichment) is a method to detect viable Legionella bacteria in building water systems. Objective To evaluate PVT VIABLE against the ISO 11731:2017 Water Quality – Enumeration of Legionella reference method for the detection of viable Legionella species in potable and non-potable water. Methods PVT VIABLE
-
A Single-laboratory Performance Evaluation of MALDI-TOF MS in Rapid Identification of Staphylococcus aureus, Cronobacter sakazakii, Vibrio parahaemolyticus, and Some Closely Related Bacterial Species of Public Health Importance J. AOAC Int. (IF 1.6) Pub Date : 2023-09-19 Irshad M Sulaiman, Nancy Miranda, Whitney Hook, Joseph Mendoza, Quincy Kumfert, Tamayo Barnes, Kidon Sung, Saeed Khan, Mohamed Nawaz, Pratik Banerjee, Steven Simpson, Kevin Karem
Background Staphylococcus is a genus of Gram-positive bacteria, known to cause food poisoning and gastrointestinal illness in humans. Additionally, the emergence of methicillin-resistant S. aureus (MRSA) strains have caused major healthcare burden worldwide. Cronobacter is a group of Gram-negative bacteria, that can survive in extreme dry conditions. Cronobacter sakazakii is known to contaminate the
-
Development and validation of novel quality evaluation methods to differentiate two closely related species of Tinospora: A rapid HPTLC and HPLC-based assessment with -MS/MS characterization J. AOAC Int. (IF 1.6) Pub Date : 2023-09-18 Aboli Girme, Ganesh Saste, Arun Kumar Balasubramaniam, Chetana Ghule, Vallabh Mulay, Lal Hingorani
Background The sympatric occurrence of the species that often resulted in different gatherings of plant material, ambiguous history on traditional use, and taxonomic flux due to similarities within the Tinospora (Menispermaceae) taxa are some of the reasons that triggered the necessity to develop robust analytical methods for efficient quality control, especially to recognize dry and powder form. Objective
-
Simultaneous determination of estradiol cypionate and medroxyprogesterone acetate hormones in injectable suspension by UV spectrophotometry based on least squares support vector machine and fuzzy inference system: Comparison with HPLC J. AOAC Int. (IF 1.6) Pub Date : 2023-09-16 Maryam Valizadeh, Zahra Ameri Braki, Erfan Smiley
Background The combination of estradiol cypionate (ECA) and medroxyprogesterone acetate (MPA) is used to prevent pregnancy in women. The analysis of the ECA and MPA combination reveals a challenge due to the strong overlap of the spectra of these compounds. Objective Spectrophotometry technique along with chemometrics methods are simple, fast, precise, and low-cost for the simultaneous determination
-
Green HPLC method for simultaneous analysis of three natural antioxidants by Analytical Quality by Design J. AOAC Int. (IF 1.6) Pub Date : 2023-09-13 Ramya Jonnalagadda, Seetharaman Rathinam, Krishnaveni Nagappan, Vinodhini Chandrasekar
Background Glutathione, Silybin, and Curcumin are well-known potential antioxidants that can are recommended as adjuvant therapy in cancer treatment. Objective Based on the principles of Analytical Quality by Design (AQbD) and green analytical chemistry, a simple, robust, and environmentally benign HPLC method for the simultaneous estimation of Glutathione, Silybin, and Curcumin in bulk and formulation
-
Stability Study and Handling Recommendations for Multiresidue Pesticide Mixes under Diverse Storage Conditions for LC-MS/MS and GC-MS/MS J. AOAC Int. (IF 1.6) Pub Date : 2023-09-12 Landon A Wiest, Jana R Hepner, Jason E Fisher, Karen M Risha, John H Lidgett, Valerie N Ballarotto, Joseph D Konschnik
Background In response to the growing global need for pesticide residue testing, laboratories must develop versatile analytical methods and workflows to produce scientifically sound results. One of the many challenges faced by food chemists is acquiring suitable pesticide certified reference materials (CRMs) to calibrate analytical equipment, monitor method performance and confirm the identity and
-
Understanding the stress testing characteristics of Apixaban, structural elucidation of novel degradation impurity, and stability indicating method development for related substances quantification J. AOAC Int. (IF 1.6) Pub Date : 2023-09-12 Srikanth Reddy Surukonti, M S Surendrababu
Background People who have non-valvular atrial fibrillation may benefit from taking a new oral anticoagulant called Apixaban, which has recently been given the green light by the Food and Drug Administration of the United States. During the stress testing that was done on it, it was found to have a high degree of degradability when subjected to both acidic and basic conditions, and one significant
-
Fluoride Determination in Milk, Soy, and Water-Based Products Using Ion-Selective Electrode and Direct Measurement Technique: Single-Laboratory Validation, First Action 2022.05 J. AOAC Int. (IF 1.6) Pub Date : 2023-09-11 Renée M Erney, Charles K Black
Background The AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals issued a call for methods for the determination of fluoride in infant formula. Standard Method Performance Requirements (SMPR®) 2014.016 were approved. Objective A single laboratory validation (SLV) of a fluoride ion selective electrode F-ISE method was completed. Method Five powder samples were reconstituted, and hydrochloric
-
Simultaneous Chromatographic Estimation of Vildagliptin and Dapagliflozin Using Hybrid Principles of White Analytical Chemistry and Analytical Quality by Design J. AOAC Int. (IF 1.6) Pub Date : 2023-09-11 Pintu Prajapati, Bageshree Rana, Veera Shakar Pulusu, Shailesh Shah
Background The fixed-dose combination of vildagliptin and dapagliflozin is used in the treatment of type 2 diabetes mellitus. According to the literature survey, RP-HPLC and HPTLC methods have been reported for routine analysis of vildagliptin and dapagliflozin. These chromatographic have been developed using potentially neurotoxic and teratogenic solvents which are unsafe for human and aquatic animal
-
A Review on Analytical Methods for Tigecycline Estimation from Its Bulk and Dosage Form J. AOAC Int. (IF 1.6) Pub Date : 2023-09-06 Bansi Rakholiya, Priyangi Shah, Yash Patel, Gayatri Patel, Samir Patel, Archita Patel
Background Tigecycline (TIG) is a third-generation glycylcycline derivative used as an antimicrobial and anticancer agent for the past few years. Its intricate structure makes it more vulnerable towards degradation under the influence of various environmental factors and leads to the generation of impurities. Due to its stability issues, TIG is available as a lyophilized powder for injection. The analysis
-
Green method for evaluation of marbofloxacin tablets by HPLC and evaluation of interchangeability with UV and turbidimetric methods J. AOAC Int. (IF 1.6) Pub Date : 2023-09-06 Aline Sinzervinch de Oliveira, Naiara Raica Lopes de Oliveira, Jerônimo Raimundo de Oliveira Neto, Layla Lopes Tavares, Ana Carolina Kogawa
Background Marbofloxacin (MAR) is a veterinary antimicrobial, marketed in injectable solution, oral suspension and tablets. MAR has no monograph for tablets evaluation in official compendiums. HPLC methods present in the literature for evaluating MAR in tablets do not follow the principles of green and sustainable analytical chemistry. Objective A green, clean and sustainable method by HPLC was developed