Abstract BackgroundColor additives requiring batch certification by the U.S. Food and Drug Administration (FDA) have Code of Federal Regulations (CFR) specification limits for certain elements and are usually analyzed by x-ray fluorescence spectrometry (XRF). However, sensitivity for Hg is too low in some color additives. ObjectiveThe thermal decomposition amalgamation-atomic absorption spectrometric

Abstract BackgroundThe candidate method, SMARTCHEK® Listeria monocytogenes Detection Kit, is designed for rapid detection of Listeria monocytogenes in select food matrixes. For use in conjunction with the Genesystems Rapi: chipTM and GENECHECKER®UF-300 real-time PCR system. ObjectiveThe independent laboratory evaluation consisted of an inclusivity and exclusivity study, product consistency study, robustness

Abstract BackgroundAlkylhydrazines are widely used in the industrial fields. An analysis of alkylhydrazines in surface water is need because these chemicals are likely to be discharged into wastewater and enter aquatic environments. ObjectiveAn ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was developed to determine the levels of five alkylhydrazines (N

Abstract BackgroundDetermination of a multi-component mixture by HPLC requires many preliminary runs for method development which is both time-consuming and expensive due to the usage of large solvent volumes. ObjectiveIn the present study, the main objective was to reduce the preliminary runs that are required for optimizing the method conditions and also shorten the run time of analysis to be suitable

Abstract BackgroundChromatographic behavior of different substances in normal and reversed phases is an interesting area in the scientific community. ObjectiveThe work aimed to optimize and validate chromatographic separation methods for simultaneous determination of paracetamol (PAR) and phenylephrine HCl (PHE) in presence of PAR impurities namely, P-Aminophenol (PAP), P-Nitrophenol (PNP), Acetanilide

Various processing aids and fining agents are used in winemaking to help improve sensory characteristics. Some of these materials may contain or be derived from allergenic foods, such as eggs. In order to ensure food safety and that products meet regulatory compliance, it is essential to have robust and effective analytical methods to verify the removal of allergenic proteins following their use. Current

Abstract BackgroundAt present, PGRs are widely used in agricultural and forestry production in the world, PGRs, like the traditional pesticides have certain toxicity, naively excessive applied them will cause the acute and chronic poisoning of humans and animals, potential harm to human health. ObjectivesIn order to assess, prevent and control the residues of plant growth regulators (PGRs) in fruits

Abstract BackgroundSulphasalazine as a prodrug, its active metabolite (mesalazine) which also is available in pharmaceutical formulations, and the major active metabolite of mesalazine; N-acetyl-5-aminosalicylic aid are commonly used for treatment of inflammatory bowel diseases. ObjectivesTwo accurate, precise, sensitive and specific spectrophotometric methods were developed and validated for determination

Abstract BackgroundIn Guangdong Province of China, the climate here is very wet, so there are many different fungus living in the aquatic feeds, which produce mycotoxins. These compounds contaminate agriculture products world-wide and represent a great threat to human health. It is necessary to determine their contamination level in aquatic feeds. ObjectiveA high performance liquid chromatography tandem

Abstract BackgroundPrednisolone is immunosuppressant and anti-inflammatory drug; it may cause peptic ulcers as a side effect. Esomeprazole is used for treatment of peptic ulcers therefore; the two drugs are co-administered in case of organ transplantation and autoimmune diseases. ObjectiveThis work aims to determine simultaneously the two drugs together in bulk and spiked human plasma by eliminating

Abstract BackgroundAdhatoda vasica L. is a medicinal plant, also known as Malabar nut in English, belongs to family Acanthaceae. It has been used traditionally to treat respiratory disorders like severe cough, cold, chronic bronchitis, asthma, tuberculosis, and other illnesses. The multifunctional range of bioactives found in it has piqued the interest of pharmaceutical companies, who are looking for

Abstract BackgroundNatural contamination of mycotoxins in dried distiller’s grains with solubles (DDGS) as a mainstream animal feed ingredient poses risk to animal health. ObjectiveA regulatory method was needed for the agency to simultaneously detect eleven mycotoxins of high regulatory priority in DDGS. MethodsTen grams of DDGS sample were extracted twice with acetonitrile/water under mildly acidic

Validated UPLC method and TLC densitometric method were prescribed for determination of antihypertensive components. Objectives: To establish and validate rapid and accurate Ultra performance liquid chromatographic (UPLC) and TLC densitometric methods for determination of Xipamide and Triamterene in pure and dosage forms.

Exposure of Apis mellifera to neonicotinoid insecticides is one of the factors attributed to the recent decline in Apis mellifera populations resulting in economic and ecological losses due to loss of pollination services. Honeybees can get exposed to neonicotinoids like imidacloprid directly in the field at the time of application as well as during consumption of pollen and nectar from treated plants

Resveratrol and emodin are abundant polyhydroxy compounds that are found in the Chinese traditional medicinal herb Polygonum cuspidatum and widely used due to their excellent antioxidant properties.

Abstract BackgroundThe dried fruit of Chaenomeles speciosa, known as Chaenomelis Fructus or Zhoupi Mugua is a type of Traditional Chinese Medicine (TCM) that is widely used to treat many diseases. In addition, Guangpi Mugua, the dried fruit of the Chaenomeles sinensis, is its most commonly known adulterant. ObjectiveTo establish a robust approach for the quality control and identification of Chaenomelis

Abstract BackgroundDrug counterfeiting is a rising problem that is difficult to identify, as well as the lack of regulations and legislation in less developing countries, represent major flaws, and this leads to the exacerbation of this serious problem. ObjectivesThis study aims to develop a robust and an economic RP-HPLC method for simultaneously determining metformin HCl, vildagliptin, saxagliptin

As a traditional Chinese health food, edible bird's nest (EBN) has high medicinal value, which is mostly attributed to the high content of sialic acid (SA). SA mainly exists in protein-bound, oligosaccharide-bound and free forms and the binding forms of SA are closely related to the functions of EBN.

A recent combination of aspirin (ASP) and omeprazole (OMP) has been presented in a fixed dosage form for treatment of many CVD, particularly in patients with gastric diseases. However, ASP is very sensitive to degradation into salicylic acid (SAL) as its main degradation product. Hence, it is very important to develop methods for the determination of ASP and OMP in the presence of SAL.

Abstract BackgroundRebamipide (REB) is quinolinone derivative compound, which is used for treatment of stomach ulcers. ObjectiveFour Spectrophotometric methods are developed for quantification of REB and its impurity and degradation product; the debenzoylated isomer of Rebamipide (DER). MethodsMethod A is ratio difference spectrophotometry (RD) where 254 and 291 nm were selected for REB and 320 and

Abstract BackgroundNitrosamine impurities are potent carcinogens in animals and probable carcinogens in humans. In current scenario, there is a need for effective analytical methods to detect and identify various nitrosamine impurities, and to develop rapid solutions to ensure the safety and quality of the drugs. ObjectiveIn present work, liquid chromatographic method was developed for estimation of

Abstract BackgroundCleaning is very important in reusable surgical instruments (RSI) reprocessing. Adenosine triphosphate (ATP) method is widely used for cleanliness assessing while few studies focused on using this method on RSIs. ObjectiveTo assess the effectiveness of ATP Bioluminescence Assay for monitoring the cleanliness of RSIs. MethodsThe study conducted in three central sterile supply departments

The U.S. Food and Drug Administration (FDA) batch certifies FD&C Yellow No. 6 (Y6) to ensure that the color additive meets requirements published in the Code of Federal Regulations, including specifications for seven organic manufacturing impurities consisting of two intermediates, a reaction by-product, an impurity originating from an intermediate, and three subsidiary colors.

Abstract BackgroundPyrrolizidine alkaloids (PAs) are naturally occurring plant toxins associated with potential hepatic and carcinogenic diseases in humans and animals. The concern of PAs has been increased as the consumption of herbal medicines is increased. ObjectiveThis study aimed to develop and validate a sensitive analytical method to determine 28 PAs in 5 herbal medicines using liquid chrom

Abstract BackgroundThe MYChrOme™ Culture Plate is a chromogenic media for the detection and differentiation of rapid-growing nontuberculous mycobacteria (NTM) in water, aided by MYCOn™ decontamination to reduce background microbiota. ObjectiveEvaluate the MYChrOme™ Culture Plates for the detection of rapid-growing NTM in potable and non-potable water as part of the AOAC Performance Tested MethodSM

Abstract BackgroundIn the recent scenario of green chemistry, the usage of organic solvent should be minimized in the development of the analytical method for the safety of the environment and analysts. ObjectiveHence, the RP-HPLC method has been developed as an economical and eco-friendly alternative to published RP-HPLC methods for the analysis of FDC of anti-hypertensive drugs to save time, resources

β-Galactooligosaccharides (GOS) are typically used in infant formula and adult nutritionals as a source of non-digestible oligosaccharides, which may bring beneficial effects through modulation of the gut microbiota. However suitable methods for the determination of GOS in products with a high background of lactose do not exist.

Alternaria toxins are ubiquitous contaminants in highly consumed food products. Therefore, they are candidates to be regulated by EU legislation. In this context the availability of reliable analytical methods is a keystone both for protecting the health of the citizens and the smooth functioning of the European market.

BACKGROUND Ivermectin is a potent semi-synthetic antiparasitic drug used in veterinary medicine. It is widely used for the treatment of parasites. OBJECTIVE This study aimed to develop a stability-indicating reversed-phase HPLC (RP-HPLC) method for assay and identification of ivermectin including identification and estimation of its related substances in bulk drug substance batches of ivermectin. METHOD

Abstract BackgroundThe candidate method, SMARTCHEK Salmonella spp. Detection Kit assay1, is designed for rapid detection of Salmonella spp. in select food matrixes. For use in conjunction with the Genesystems Rapi: chipTM and GENECHECKERUF-300 real-time PCR system. ObjectiveThe independent laboratory evaluation consisted of an inclusivity and exclusivity study, product consistency study, robustness

Abstract BackgroundThe concomitant montelukast and bilastine are used as additive therapy for seasonal allergic rhinoconjuctivitis and mild to moderate asthma. According to the literature review, no UV-visible spectrophotometry method has been reported yet for simultaneous estimation of montelukast sodium and bilastine in their combined pharmaceutical dosage forms. ObjectivesHence, five different multicomponent

Abstract BackgroundThe testing of aflatoxins (AFs) in fresh and processed foods is highly demanded to comply with trade regulations. Consequently, commercial laboratories face huge AF sample loads in food consignments. Worldwide, there is a rising interest to implement automation to increase sample throughput in AF analysis. ObjectiveThis study sought to evaluate the performance of an automated cleanup

The wide livestock usage of tetracyclines may result in drug residues in foods. Therefore, it is necessary to develop reliable methods for the determination of tetracyclines in foods.

Four rapid, smart, and easy to apply spectrophotometric methods were displayed for the estimation of Solifenacin succinate (SOL) in existence of its acid inspired degradation product.

Abstract BackgroundBoth deficient and excessive intake of fluoride can lead to adverse health problems. ObjectiveThe aim of this study is to investigate the fluoride content in three types of drinking water in Suzhou urban area. MethodIon chromatography was employed to conduct the validation of analysis of fluoride ion in water and the method was applied to analyze the concentration of fluoride in

Cocoa butter, an expensive product containing a specific triacylglycerol composition, is often the object of economic adulteration.

Green chemistry approach was developed for the purpose of saving the environment by using green solvents. Applying the green analytical chemistry principles for traditional methods is considered a challenge. Acemetacin is a commonly used analgesic prodrug that bioactivated to indomethacin.

Abstract BackgroundThe Clear Safety Listeria method utilizes polymerase chain reaction (PCR) amplification and targeted Next-Generation Sequencing (NGS) technology to detect Listeria species (L. monocytogenes, L. innocua, L. ivanovii, L. marthii, L. grayi, L. welshimeri, and L. seeligeri) in hot dogs and on selected environmental surfaces. ObjectiveValidate the candidate method according to current

Hypertension is a major health problem found in people throughout the world and numerous fixed-dose combination products of telmisartan are available in the local market for the treatment of hypertension. Several chromatography methods such as RP-HPLC and HPTLC methods have been published for estimation of FDC products of telmisartan with hydrochlorothiazide, atorvastatin, and amlodipine. But no RP-HPLC

Levofloxacin is a third-generation fluoroquinolone that has several advantages over its R-Ofloxacin isomer. It is used to treat different types of infections including urinary and prostatitis.

Abstract BackgroundBosutinib is a small molecule BCR-ABL, and src tyrosine kinase inhibitor used for the treatment of chronic myelogenous leukaemia. According to published literature, no stability-indicating RP-HPLC method has been reported yet for estimation of bosutinib. ObjectiveHence, the stability-indicating RP-HPLC method has been developed for the stability study of bosutinib using risk and

Abstract BackgroundDiflunisal (DIF) has analgesic and anti-inflammatory activity. It is a pharmacopeial drug found in the British Pharmacopoeia, and its major pharmacopeial impurity is biphenyl-4-ol (BPL). ObjectiveDiflunisal was not determined before together with BPL. Presence of BPL could significantly affect the dose of DIF in its dosage forms; hence it is crucial to determine DIF and BPL in presence

Abstract BackgroundBulbine natalensis Baker and Bulbine frutescens (L.) Willd. belonging to the family Asphodelaceae are widely distributed in South Africa and traditionally used as an aphrodisiac and skin remedies. ObjectiveThe aim of this study is to develop an analytical method for chemical profiling and identification of components in Bulbine species, which would be useful for herbal identification

Abstract BackgroundPholidota articulata Lindl has been used as a traditional medicine and Yi-nationality herbal medicine for the treatment of various diseases. Phenanthrenes and bibenzyls commonly found in genus Pholidota are one of the most important natural ingredients owing to their various biological activities. ObjectiveThis study aimed to establish a HPLC method for determination of the levels

Abstract BackgroundAnhydrous milk fat can be obtained in two forms, Ghee and butter oil. These products have been made by boiling butter at 110–120 °C and melting butter at 60–80 °C, respectively, to give them their distinct flavor. Ghee is the most popular fatty dairy product in India and Egypt. Lipid oxidation is a major cause of ghee and butter oil deterioration, which affects greatly the flavor

Extraction is the leading critical stage in the analysis of nutraceuticals. Ginkgo biloba (GB) has gained an interest because of its therapeutic usages.

Numerous chromatography methods have been published for estimation of fixed-dose combinations (FDC) products of aspirin and ramipril with other drugs. But no published report has been found which promotes simultaneous estimation of some FDC of aspirin and ramipril with other drugs using a single chromatography condition.

Clostridium is a genus of Gram-positive, spore-forming, anaerobic bacteria comprising approximately 100 species. Some Clostridium spp. (C. botulinum, C. perfringens, C. tetani and C. difficile) were recognized to cause acute food poisoning, botulism, tetanus, and diarrheal illness in humans. Thus, rapid identification of Clostridium spp. is critical for source tracking of contaminated food and to understand

Eprinomectin is used as an active pharmaceutical ingredient (API) in various drug products by the animal health industry. Several major related impurities of Eprinomectin API are not separated and coelute by the current United States Pharmacopeia method for Eprinomectin.

Buddleja lindleyana Fort., which belongs to the Loganiaceae with a distribution throughout the tropics, is widely used as an ornamental plant in China. Buddleja contains several morphologically similar species, which need to be identified by molecular identification. But there is little molecular research on the genus Buddleja.

The Clear Safety Salmonella method was modified to improve sample preparation, PCR reagents, library preparation, flow cell quality control, library loading mix, priming mix and sequencing kit reagents and steps.

Abstract BackgroundMorocco is an important world producer and consumer of several varieties of date palm. In fact, the discrimination between varieties remains difficult and requires the use of complex and high-cost techniques. ObjectiveWe evaluated in this work the potential of mid-infrared spectroscopy (MIR) and chemometric models to discriminate eight date palm varieties. MethodsFour chemometric

Ocimum genus known as Tulsi or Basil is a prominent botanical class in Asian culture, especially in India. The leaves have an immunomodulatory, antioxidant, stress-relieving, and adaptogenic role in traditional and modern medicine, with prominent usage in herbal teas and nutraceuticals.

We would like to thank the following individuals who have reviewed manuscripts for the Journal of AOAC INTERNATIONAL in 2020

BACKGROUND The Eclipse Farm 4 G test coupled to the COMET device allows for automatic and easy screening of a broad range of antimicrobials in raw bovine milk. All results obtained with this system are available in real-time through a smartphone application (App) and in the Test4all cloud platform. OBJECTIVE The objective of this study was to validate Eclipse Farm 4 G & COMET for the screening of antimicrobials

BACKGROUND The Eclipse Farm 4 G test coupled to the COMET device allows for automatic and easy screening of a broad range of antimicrobials in raw bovine milk. All results obtained with this system are available in real-time through a smartphone application (App) and in the Test4all cloud platform. OBJECTIVE The objective of this study was to validate Eclipse Farm 4 G & COMET for the screening of antimicrobials

BACKGROUND Sangbaipi Decoction(SD), a traditional Chinese "Ancient Classical Prescriptions" consisting of eight medicinal materials, is usually used for asthma due to excess lung Qi and excessive phlegm-fire. OBJECTIVE To establish a comprehensive quality evaluation methods of SD. MATERIALS AND METHODS High performance liquid chromatography (HPLC) fingerprint analysis, hierarchical cluster analysis

BACKGROUND Sangbaipi Decoction(SD), a traditional Chinese "Ancient Classical Prescriptions" consisting of eight medicinal materials, is usually used for asthma due to excess lung Qi and excessive phlegm-fire. OBJECTIVE To establish a comprehensive quality evaluation methods of SD. MATERIALS AND METHODS High performance liquid chromatography (HPLC) fingerprint analysis, hierarchical cluster analysis

Roses are flowers which contain various phytochemicals and have been used for food and medicinal purposes in many countries. Materials, including flowers, are available for limited periods when plants can be cultivated.