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Development and Validation of a Multi-residue Analytical Method for Veterinarian and Human Pharmaceuticals in Livestock Urine and Blood Using UHPLC-QTOF J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-26 Xue Wang; Ke-wen Wang; Zhen-zhen Xu; Shu-ming Yang; Yan zhao
The aim of this work is to contribute to the assessment of multi-residue analysis of veterinarian and human pharmaceuticals using UHPLC-QTOF in livestock urine and blood (cattle, chicken, sheep and pig). Firstly, an in-house database including compound name, monoisotopic mass, chemical formula, retention time, chemical structure, and three CID MS-MS spectra of the 234 selected drugs were built for
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Untargeted metabolomics reveals the synergistic mechanisms of Yuanhu Zhitong oral liquid in the treatment of primary dysmenorrhea J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-05 Kai Zhang; Jing Su; Yuting Huang; Yingchao Wang; Qingfen Meng; Jianli Guan; Shixin Xu; Yi Wang; Guanwei Fan
Primary dysmenorrhea is a prevalent gynecological disorder that severely affects the quality of life in women. Yuanhu Zhitong oral liquid (YZOL) is a standardized herbal preparation frequently used in clinical practice and is a promising alternative therapy for primary dysmenorrhea. The findings of previous studies show that YZOL exhibits significant analgesic and spasmolytic effects, however, the
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Determination of 3-iodothyronamine (3-T1AM) in mouse liver using liquid chromatography-tandem mass spectrometry J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-18 Zhong-Min Li; Manuel Miller; Sogol Gachkar; Jens Mittag; Sonja C. Schriever; Paul T. Pfluger; Karl-Werner Schramm; Meri De Angelis
3-iodothyronamine (3-T1AM) has been suggested as a novel chemical messenger and potent trace amine-associated receptor 1 ligand in the CNS that occurs naturally as endogenous metabolite of the thyroid hormones. Discrepancies and variations in 3-T1AM plasma and tissue concentrations have nonetheless caused controversy regarding the existence and biological role of 3-T1AM. These discussions are at least
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Volumetric absorptive microsampling for the quantification of tacrolimus in capillary blood by high performance liquid chromatography-tandem mass spectrometry J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-05 Camille Tron; Marie-José Ferrand-Sorre; Julie Querzerho-Raguideau; Jonathan M. Chemouny; Pauline Houssel-Debry; Marie-Clémence Verdier; Eric Bellissant; Florian Lemaitre
Volumetric absorptive microsampling (VAMS) is an innovative alternative strategy to venipuncture for monitoring tacrolimus levels in transplant recipients. In this study, we aimed to validate a new high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for quantifying tacrolimus in blood collected by VAMS. Tacrolimus was extracted from dried blood tips in an original process
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Screening and identification of bioactive components resistant to metallo-beta-lactamase from Schisandra chinensis (Turcz.) Baill. by metalloenzyme-immobilized affinity chromatography J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-05 Meijiao Duan; Jiakun Bai; Jian Yang; Penghui Shi; Liujiao Bian
The screening and identification of bioactive components, which are effectively resistant to metallo-beta-lactamase (MβL), were studied in the alcohol extract of Schisandra chinensis (Turcz.) Baill. by metalloenzyme-immobilized affinity chromatography. Taking bizinc metalloenzyme beta-lactamase II from Bacillus cereus (Bc II) and monozinc metalloenzyme CphA from aeromonas hydrophila (CphA) as examples
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A terminal alkyne and disulfide functionalized agarose resin specifically enriches azidohomoalanine labeled nascent proteins J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-07 Zhanwu Hou; Xiao Han; Zhen Wang; Sana Ghazanfar; Jeffy Yang; Huadong Liu
Nascent proteome presents dynamic changes in response to a certain stimulus. Thus, monitoring nascent proteome is critical to uncovering the involved biological mechanism. But the low-abundance of nascent proteome against an overwhelming pre-existing proteome limits its identification and quantification. Herein, we present a novel strategy to enrich nascent proteome from whole cell lysate for further
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Chiral liquid chromatography-tandem mass spectrometry analysis of superwarfarin rodenticide stereoisomers – Bromadiolone, difenacoum and brodifacoum – In human plasma J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-07 Daniel G. Nosal; Douglas L. Feinstein; Richard B. van Breemen
Superwarfarins are second-generation long-acting anticoagulant rodenticides that can cause unintended human and wildlife toxicity due, in part, to their prolonged half-lives. Commercially available superwarfarin rodenticides are synthesized as racemates with two asymmetric carbons, producing four stereoisomers. To support studies of human plasma half-lives of individual superwarfarin stereoisomers
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Fe3O4 nanoparticles as matrix solid-phase dispersion extraction adsorbents for the analysis of thirty pesticides in vegetables by ultrahigh-performance liquid chromatography-tandem mass spectrometry J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-09 Jin Liu; Chunhong Ji; Xiaoliang Liu; Xuesheng Li; Haiyan Wu; Dongqiang Zeng
Herein we report the first example of Fe3O4 nanoparticles (FNPs) being used as single-matrix solid-phase dispersion (MSPD) adsorbents for the extraction of 30 representative pesticides from vegetables. This study was aimed at analyzing the extracted samples using ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). Various condition parameters, such as the eluent, volume
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Performance of phospho-L-tyrosine immobilized onto alginate/polyacrylamide-based cryogels: Effect of ligand coupling on human IgG adsorption and Fab fragments separation J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-06 Camila Marcuz; Cecília Alves Mourão; Karsten Haupt; Sonia Maria Alves Bueno
The ortho-phospho-tyrosine (P-Tyr) pseudoaffinity ligand was immobilized via ether linkage onto polyacrylamide-alginate (PAAm-Alg)-epoxy cryogels prepared according to two different approaches in order to explore their performance in the immunoglobulin G (IgG) purification from human serum. In the first approach, the P-Tyr was attached to cryogel prepared by cryocopolymerization of acrylamide and alginate
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LC-HRMS and acetylcholinesterase affinity assay as a workflow for profiling alkaloids in Annona salzmannii extract J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-15 Juliana M. Lima; Gabriel M. Leme; Emmanoel V. Costa; Quezia B. Cass
Structure-based molecular networking is useful as a dereplication strategy to identify known molecules, unknown close analogues, or compound families. On the other hand, the ligand fishing assay is a remarkable alternative to accelerate the screening process and to overcome the drawbacks of laborious experiments usually adopted in natural product research. The combination of these approaches contributes
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Development and validation of an LC-MS/MS method for the bioanalysis of psilocybin’s main metabolites, psilocin and 4-hydroxyindole-3-acetic acid, in human plasma J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-07 Karolina E. Kolaczynska; Matthias E. Liechti; Urs Duthaler
Psilocin is the active metabolite of psilocybin, a serotonergic psychedelic substance. It is used recreationally and investigated in substance-assisted psychotherapy. The pharmacokinetic properties of psilocin are only partially characterized. Therefore, we developed and validated a rapid LC-MS/MS method to quantify psilocin and its metabolite 4-hydroxyindole-3-acetic acid (4-HIAA) in human plasma
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Determination of estrogens and estrogen mimics by solid-phase extraction with liquid chromatography-tandem mass spectrometry J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-20 Yejin Li; Linyan Yang; Huajun Zhen; Xueming Chen; Mei Sheng; Kai Li; Weibo Xue; Huihui Zhao; Shujuan Meng; Guomin Cao
An analytical method has been developed and validated for the determination of six estrogens and estrogen mimics, namely estriol (E3), bisphenol A (BPA), 17β-estradiol (E2), estrone (E1), ethynyl estradiol (EE2) and dienestrol (DIE), with frequent occurrence in the natural environment. Solid phase extraction coupled with liquid chromatography tandem mass spectrometry (SPE-LC-MS/MS) using electrospray
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Simultaneous separation of insulin and six therapeutic analogues on a mixed mode column: HPLC-UV method development and application J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-20 Anja Kristl; Aleš Podgornik; Matevž Pompe
Human insulin and six most used therapeutic analogues are very similar in terms of retention on a reversed-phase column. Thus, LC methods prescribed in the European Pharmacopoeia monographs for insulin and insulin analogues include many similar separation methods, which tend to be time consuming when separating individual products of insulins or are inadequate when handling a mixture. In this study
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In depth Investigation of the Retention Behavior of Structurally related β-Blockers on RP-HPLC column: Quality by Design and Quantitative Structure-Property Relationship Complementary approaches for Optimization and Validation J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-20 Ahmed Faried Abdel Hakiem; Ahmed K. Hamdy; Hassan Refat Hassan Ali; Mohamed Gomaa; Ahmed Safwat Aboraia
The persistent introduction of new β-blockers motivates the demand for optimizing RP-HPLC well-designed analytical procedures that could be applied to this structurally related and commonly prescribed pharmacological group in order to reduce time and chemicals consumption in quality control units. Betoxolol HCl (BET) and Carvidolol (CAR) were selected as representative examples to conduct predictive
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Development and validation of an LC-MS/MS method for the quantification of the anthelmintic drug moxidectin in avolumetric absorptive microsample, blood, and plasma: Application to a pharmacokinetic study of adults infected with Strongyloides stercoralis in Laos J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-20 Daniela Hofmann; Somphou Sayasone; Jennifer Keiser
Moxidectin is a promising candidate for addition to the lean repertoire of drugs against neglected tropical diseases (NTD) including strongyloidiasis. Pharmacokinetic (PK) and -dynamic studies are required to support its clinical development. Microsampling approaches enable PK studies in the challenging environments where NTDs are most prevalent, due to simplified collection and processing. We developed
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Retention-directed and selectivity controlled chromatographic resolution: Rapid post-hoc analysis of DMPK samples to achieve high-throughput LC-MS separation J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-24 Paul Turnpenny; Anthony Dickie; Jed Malec; Jordan McClements
High quality chromatographic separation underpins robustness in LC-MS, frequently the analytical method of choice for pharmaceutical drug discovery work. The potential improvements in chromatographic selectivity afforded by serial column coupling (SCC), provide a useful means to enhance the resolution of complex samples. In this work, we present a revised high-throughput form of SCC, in which just
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Development of extraction separation technology based on deep eutectic solvent and magnetic nanoparticles for determination of three sex hormones in milk J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-19 Zexin Zhao; Chu Liu; Jiaxin Lian; Ning Liang; Longshan Zhao
A rapid, reliable and eco-friendly method for the determination of three sex hormones in five kinds of milk was developed and validated by combining vortex-assisted liquid-liquid microextraction (VALLME) and magnetic solid-phase extraction (MSPE). Deep eutectic solvents (DESs) such as choline chloride/urea were considered as the extraction solvent in VALLME and multi-walled carbon nanotubes (MMWCNTs)
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Modified μ-QuEChERS coupled to diethyl carbonate-based liquid microextraction for PAHs determination in coffee, tea, and water prior to GC-MS analysis: An insight to reducing the impact of caffeine on the GC-MS measurement J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-19 Asmaa Kamal El-Deen; Kuniyoshi Shimizu
A fast, sensitive and eco-friendly method was developed for the determination of fifteen polycyclic aromatic hydrocarbons (PAHs) in different environmental matrices through gas chromatography mass spectrometry (GC-MS). The method utilizes a modified and miniaturized quick easy cheap effective rugged and safe (QuEChERS) clean up procedure coupled to an air-assisted dispersive liquid-liquid microextraction
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Bioanalysis of erlotinib, its O-demethylated metabolites OSI-413 and OSI-420, and other metabolites by liquid chromatography-tandem mass spectrometry with additional ion mobility identification J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-18 Johannes J.M. Rood; Javier Sastre Toraño; Victor J. Somovilla; Jos H. Beijnen; Rolf W. Sparidans
Erlotinib is a first-generation epithelial growth factor receptor inhibitor used in the treatment of non-small cellular lung cancers. Our previously published method on a Thermo TSQ Quantum Ultra triple quadrupole mass spectrometer for the quantitation of erlotinib, OSI-420, and OSI-413 and some other kinase inhibitors was transferred to a more sensitive Sciex QTRAP5500 system. Both methods showed
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Simultaneous quantification of cefuroxime and clindamycin in human lumbar anulus fibrosus, nucleus pulposus and serum via UPLC-MS/MS J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-18 Xiufang Liang; Zhongping Gou; Xiandi Wang; Yongsheng Wang; Jiao Yue; Na Li; Ping Feng; Yongping Qin; Jiancheng Zeng
Background This study aimed to develop a sensitive, accurate method for simultaneously quantifying cefuroxime and clindamycin in human serum, lumbar anulus fibrosus and nucleus pulposus. Methods Cefuroxime and clindamycin were quantified using ultra high-performance liquid chromatography-electrospray ionization tandem mass spectrometry in multiple-reaction-monitoring mode on a triple-quadrupole AB
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Simultaneous Determination of a Novel Oxazolidinone Anti-tuberculosis OTB-658 and Its Metabolites in Monkey Blood by LC-MS/MS J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-18 Jianwei Jiang; Yuke Liu; Xiao Liu; Dongfeng Zhang; Haihong Huang; Baolian Wang; Li Sheng; Yan Li
OTB-658, a novel oxazolidinone anti-tuberculosis agent, has potent antibacterial activity against Mycobacterium tuberculosis, especially multi-drug resistant tuberculosis (MDR-TB) in vitro and in vivo. In this study, after metabolite identification of parent drug OTB-658, a specific and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established and validated to quantify
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Enantiomeric resolution of quinolones on crown ether CSP: Thermodynamics, chiral discrimination mechanism and application in biological samples J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-16 Zeid A. ALOthman; Ahmad Yacine Badjah; Khalid M. Alsheetan; Mohd. Suhail; Imran Ali
The enantiomers of quinolone racemates were resolved using chiral crown ether within 8 minutes. Thermodynamics data and modeling results were used to determine chiral recognition mechanism. The column used was (+)-Crownpack column (250 mm × 4.6 mm, 5 µm) with three mobile phases I: ACN:Water (80:20) + 10 mM H2SO4 and 10 mM CH3COONH4, II: ACN:Water (80:20) + 20 mM perchloric acid and III: EtOH:Water
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A perspective on the standards describing mass spectrometry-based metabolic phenotyping (metabolomics/metabonomics) studies in publications J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-24 Ian D. Wilson; Georgios Theodoridis; Christina Virgiliou
As metabolic phenotyping (metabolomics, metabonomics and also lipidomics) gains in popularity and new investigators enter the field, the need to maintain and improve standards in publication is ever more pressing. In this perspective the requirements for information that should be included in manuscripts published in the Journal of Chromatography B, to ensure that the work is both credible and repeatable
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A targeted ultra performance liquid chromatography – Tandem mass spectrometric assay for tyrosine and metabolites in urine and plasma: Application to the effects of antibiotics on mice J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-22 Marine P.M. Letertre; Antonis Myridakis; Luke Whiley; Stéphane Camuzeaux; Matthew R. Lewis; Katie E. Chappell; Annie Thaikkatil; Marc-Emmanuel Dumas; Jeremy K. Nicholson; Jonathan R. Swann; Ian D. Wilson
Tyrosine plays a key role in mammalian biochemistry and defects in its metabolism (e.g., tyrosinemia, alkaptonuria etc.) have significant adverse consequences for those affected if left untreated. In addition, gut bacterially-derived p-cresol and its metabolites are of interest as a result of various effects on host xenobiotic metabolism. A fit-for-purpose quantitative ultra-performance liquid chromatography
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Doping control analysis of small peptides: A decade of progress J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-15 Péter Judák; Simone Esposito; Gilles Coppieters; Peter Van Eenoo; Koen Deventer
Small peptides are handled in the field of sports drug testing analysis as a separate group doping substances. It is a diverse group, which includes but is not limited to growth hormone releasing-factors and gonadotropin-releasing hormone analogues. Significant progress has been achieved during the past decade in the doping control analysis of these peptides. In this article, achievements in the application
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Development and validation of a UPLC-MS/MS method for the simultaneous determination of gamma-aminobutyric acid and glutamic acid in human plasma J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-28 Tessa H. de Bie; Renger F. Witkamp; Maarten A. Jongsma; Michiel G.J. Balvers
Gamma-aminobutyric acid (GABA) and its precursor glutamic acid are important neurotransmitters. Both are also present in peripheral tissues and the circulation, where abnormal plasma concentrations have been linked to specific mental disorders. In addition to endogenous synthesis, GABA and glutamic acid can be obtained from dietary sources. An increasing number of studies suggest beneficial cardio-metabolic
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Integrating metabolomics and network pharmacology to explore Rhizoma Coptidis extracts against sepsis-associated acute kidney injury J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-05 Yuenan Zheng; Xuan Shi; Jiebin Hou; Songyan Gao; Yufan Chao; Jiarong Ding; Long Chen; Yong Qian; Guojian Shao; Yachen Si; Wei Chen
Sepsis remains the most common cause of acute kidney injury (AKI) in critically ill patients, increasing the risk of in-hospital and long-term death. Rhizoma Coptidis (RC), a classical traditional Chinese herb, exhibits anti-inflammatory and antioxidant properties in various diseases including sepsis. This study aimed to investigate the protective effects of RC extracts (RCE) against sepsis-associated
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A convenient sampling and noninvasive dried spot method of uric acid in human saliva: Comparison of serum uric acid value and salivary uric acid in healthy volunteers and hyperuricemia patients J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-06 Yu Han; Qing Shi; Chun-Yan Xu; Lei Di; Li-Li Zhao; Wenlong Jin; Jun Zhe Min
Concentration of uric acid (UA) in serum is one of the markers used to diagnose gout and hyperuricemia. However, serum treatment and storage are cumbersome, and wounds are susceptible to infection. Therefore, a new sampling and analysis method using noninvasive biological samples has been developed, called the dried spot method of UA in human saliva (DSM-UHS). Saliva (5 μL) was dropped on filter paper
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Validation of a HPLC-UV method for the quantification of budesonide in skin layers J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-24 Anna Demurtas; Silvia Pescina; Sara Nicoli; Patrizia Santi; Daniele Ribeiro de Araujo; Cristina Padula
A simple and sensitive HPLC method for the quantification of budesonide in skin layers was developed and validated. Budesonide was extracted from stratum corneum, epidermis and dermis by means of a mixture of acetonitrile:water (recovery > 90%). Budesonide quantification was performed with a RP-C18 column using methanol and water mixture (69:31, v/v) as mobile phase, pumped at 0.8 ml/min. The absorbance
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Determination of selected water-soluble vitamins (thiamine, riboflavin, nicotinamide and pyridoxine) from a food matrix using hydrophilic interaction liquid chromatography coupled with mass spectroscopy J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-12 Kate Porter; John K Lodge
Water-soluble vitamins are essential dietary components with a multitude of important functions that require quantification from food sources to characterise the nutritional status of food. In this study, we have developed a hydrophilic interaction chromatography (HILIC) based method coupled to single-quadrupole mass spectrometry (MS) for the analysis of selected water-soluble vitamins. Due to their
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New sample preparation method to analyse 15 specific and non-specific pesticide metabolites in human urine using LC-MS/MS J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-11 Petra Stuchlík Fišerová; Jiří Kohoutek; Céline Degrendele; Mohamed Aqiel Dalvie; Jana Klánová
This study presents a novel sample preparation method for the determination of both specific and non-specific pesticide metabolites in human urine samples. The method combines a deconjugation step with QuEChERS-based method and solid-phase extraction. In total, 15 pesticide metabolites (diethyl phosphate; diethyl thiophosphate; dimethyl phosphate; diethyl thiophosphate; 2,4-dichlorophenoxyacetic acid;
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Automated Mass Spectrometry Multi-Attribute Method Analyses for Process Development and Characterization of mAbs J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-11 Yvonne Ehwang Song; Hervé Dubois; Martin Hoffmann; Stephen D́Eri; Yann Fromentin; Jan Wiesner; Anja Pfenninger; Séverine Clavier; Annette Pieper; Laurent Duhau; Udo Roth
More than 370 biotherapeutics drug products have been approved by regulatory agencies on the US and EU markets and this industry continues to expand. Process change and optimization is necessary to develop new effective biologics in a cost effective and productive way. Consequently, improvement of analytical techniques is required for better product characterization according to Quality by Design (QbD)
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Integrated plasma and liver gas chromatography mass spectrometry and liquid chromatography mass spectrometry metabolomics to reveal physiological functions of sodium taurocholate cotransporting polypeptide (NTCP) with an Ntcp knockout mouse model J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-11 Qisong Zhang; Zhuoru He; Zhongqiu Liu; Lingzhi Gong
Sodium taurocholate cotransporting polypeptide (NTCP) is an important hepatocyte transporter, while its physiological functions require further investigation. In our study, an integrated plasma and liver GC-MS- and LC-MS-based metabolomics strategy with an optimized two-step liquid-liquid extraction was utilized to explore the physiological functions of NTCP via a knockout (KO) mouse model. The present
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Development of sol-gel phenyl/methyl/poly (dimethylsiloxane) sorbent coating for fabric phase sorptive extraction and its application in monitoring human exposure to selected polycyclic aromatic hydrocarbons using high performance liquid chromatography-fluorescence detection J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-28 Isil Gazioglu; Abuzar Kabir; Ozge S. Zengin; Evrim Kepekci Tekkeli; Kenneth G. Furton; Angela Tartaglia; Marcello Locatelli
Following the convenient, yet very powerful pathway to create designer extraction sorbent using sol-gel chemistry, a novel sol-gel phenyl/methyl/poly(dimethylsiloxane) sorbent coating was created on polyester fabric substrate for fabric phase sorptive extraction (FPSE) and was subsequently applied to monitor human exposure to selected polycyclic aromatic hydrocarbons (PAHs) including pyrene, chrysene
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Simple and fast LC-MS/MS method for quantification of terazosin in human plasma and application to bioequivalence study J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-28 Gabriel Onn Kit Loh; Emily Yii Ling Wong; Yvonne Tze Fung Tan; Loke Meng Ong; Ru Shing Ng; Hong Chin Wee; Kok Khiang Peh
A simple, fast and sensitive LC-MS/MS method was developed to quantify terazosin in human plasma. The mobile phase consisted of acetonitrile-0.1% (v/v) formic acid (70:30, v/v). Prazosin was used as internal standard (IS). As deproteinization agent, acetonitrile produced a clean sample. A higher response intensity with more symmetrical peak was obtained using Agilent Poroshell 120 EC-C18 – Fast LC
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Simple and sensitive quantification of glutathione hydropersulfide alkylated using iodoacetamide by high-performance liquid chromatography with post-column derivatization J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-25 Seiya Nagai; Ichiro Koshiishi
A novel analytical method was developed for the quantification of glutathione hydropersulfide (G-SSH) in biological samples by high-performance liquid chromatography (HPLC) with post-column derivatization. G-SSH was treated with iodoacetamide as an alkylating agent for 5 min at 37 °C, and the resultant acetamide-labeled G-SSH (G-SS-acetamide) was subjected to HPLC. After separation on a reversed-phase
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Simultaneous determination of a suite of endogenous steroids by LC-APPI-MS: Application to the identification of endocrine disruptors in aquatic toxicology J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-24 Brett R. Blackwell; Gerald T. Ankley
Exposure to endocrine-disrupting compounds (EDCs) can alter steroid hormone production in vertebrates, sometimes leading to adverse reproductive or developmental effects. Liquid chromatography mass spectrometry methods are the gold standard for analyte confirmation and quantification in biological matrices, but radioimmunoassays (RIAs) are most commonly used for measurement of select steroid hormones
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Optimization and Validation of a Monoclonal Antibody and its Related Fab Fragment in Human Plasma after Intravitreal Administration J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-09 Catherine E. DelGuidice; Omnia A. Ismaiel; William R. Mylott; Matthew S. Halquist
As biologic based drugs become an increasingly important sector of the pharmaceutical industry, accurate and precision techniques for bioanalysis are required to support clinical trials and beyond. Ranibizumab, a fab therapeutic, is an FDA approved drug to treat wet age-related macular degeneration (AMD), as well as other eye related diseases. Ranibizumab’s mAb counterpart, bevacizumab, is often also
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Development of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the analysis of tryptic digest of human hemoglobin exposed to sulfur mustard J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-25 Florine Hallez; Audrey Combès; Charlotte Desoubries; Anne Bossée; Valérie Pichon
Sulfur mustard is a highly reactive chemical warfare agent that causes severe damages to the victims exposed by alkylating multiple biomolecules such as proteins. Resulting alkylated products can be used as biomarkers of exposure to this chemical agent. A liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method was thus developed to detect alkylated peptides after the tryptic digestion
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Antibodies purification from human plasma using fractionation, chromatography and gel electrophoresis assisted by multivariate analysis of complimentary absorption and fluorescence spectra J. Chromatogr. B (IF 3.004) Pub Date : 2021-01-06 Javad Ghorbani; Mohsen Kompany-Zareh; Elham Tahmasebi
Employing simple precipitation (fractionation) using Cohn method and weak anion exchange chromatography with DEAE resin, antibodies such as Immunoglobulin G are purified from human plasma. Fractions are eluted from column in four different regions depending on washing NaCl concentrations. Absorbance and excitation-emission fluorescence spectral data are measured for separated chromatographic fractions
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Visualized heterooligomeric subunit structures of 817 human cellular proteins by correlating native protein 2D maps with protein interaction databases J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-21 Ya Jin; Takashi Manabe
Previously, we have reported on the reconstruction of 4323 native protein 2D maps of human bronchial smooth muscle cells (HBSMC) by combining nondenaturing 2DE, grid gel-cutting, and quantitative LC-MS/MS. In this work, we report on the visualization of the heterooligomeric subunit structures of HBSMC proteins by correlating the native protein 2D maps with the information in protein interaction databases
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Metabolic analysis of the illegal analogues of anti-obesity drugs using LC-Q-TOF-MS/MS J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-19 Daseul Jeong; Sanggil Choe; Sooyeun Lee; Kang Min Kim; Jaesung Pyo
With an increase in the obese population, the indiscriminate demand for anti-obesity drugs for rapid weight loss or maintenance has grown. As a result, illegal substances that could induce unexpected negative health effects or fatal side effects are being produced and mixed into consumer products. In the present study, the metabolites of five major illegal anti-obesity drugs are analyzed for the first
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Comparative two-dimensional GPC3 overexpressing SK-Hep1 cell membrane chromatography /C18/ time-of-flight mass spectrometry for screening selective GPC3 inhibitor components from Scutellariae Radix J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-15 Chun Chen; Yanqiu Gu; Rong Wang; Xinyi Chai; Shuya Jiang; Shaozhan Wang; Zhenyu Zhu; Xiaofei Chen; Yongfang Yuan
Screening active components targeting membrane proteins is important for drug discovery from traditional Chinese medicine. Cell membrane chromatography (CMC) has achieved a wide application in screening active components on pathological cells due to its high sensitivity and effectiveness. However, it is hard to clarify the specific target protein through simply using pathological and normal cells.
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Proteomic fingerprinting of protein corona formed on PEGylated multi-walled carbon nanotubes J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-19 Maria Nicoletti; Cristian Gambarotti; Elisa Fasoli
In Nanomedicine, carbon-based nanomaterials, like Carbon Nanotubes (CNT), are considered potential candidates as drug delivery systems. In vivo adsorption of physiological proteins onto carbon nanotubes, through noncovalent interactions, forms a protein corona or bio corona, able to influence biological properties and biocompatibility of CNT. This study aimed to explore the composition of protein corona
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Nucleic acid sample preparation from whole blood in a paper microfluidic device using isotachophoresis J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-13 Benjamin P. Sullivan; Andrew T. Bender; Duy N. Ngyuen; Jane Yuqian Zhang; Jonathan D. Posner
Nucleic acid amplification tests (NAATs) are a crucial diagnostic and monitoring tool for infectious diseases. A key procedural step for NAATs is sample preparation: separating and purifying target nucleic acids from crude biological samples prior to nucleic acid amplification and detection. Traditionally, sample preparation has been performed with liquid- or solid-phase extraction, both of which require
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Development and validation of a new UPLC-MS/MS method for quantification of ganoderic acid-A loaded nanolipidic carrier in rat plasma and application to pharmacokinetic studies J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-18 Mahfoozur Rahman; Waleed H. Almalki; Imran Kazmi; Obaid Afzal; Hanadi A. Katouah; Majed Alrobaian; Abdulmalik Saleh Alfawaz Altamimi; Fahad A. Al-Abbasi; Mohammed S. Alshammari; Rehan Abdur Rub; Sarwar Beg; Vikas Kumar
A systematic methodology was used to quantify ganoderic acid-A (GA-A) loaded nano-lipid carriers (NLC) in rat plasma using UPLC-MS/MS. Separation of the analyte was achieved using ACQUITY UPLC BEH C18 column (1.7 µm) and mobile phase as water containing 0.1% Acetonitrile (40: 60% v/v) at a flow rate of 0.4 mL·min−1. The analyte was detected using MRM mode to track precursor-to-product ion transitions
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Identification and characterization of serum albumin covalent adduct formed with atrazine by liquid chromatography mass spectrometry J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-17 Shaogang Chu; Robert J. Letcher
The present study developed an analytical technique to investigate the possible covalent adduct formation of albumin with the herbicide atrazine, and to characterize the protein modifications in vitro using liquid chromatography separation coupled with high resolution time-of-flight mass spectrometry (LC-TOF-MS). Tandem mass spectrum analysis (MS/MS) with collision induced dissociation (CID) revealed
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An UPLC-MS/MS method for quantification of D-pinitol in rat plasma and its application to a pharmacokinetic and bioavailability study J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-31 Jieying Qiu; Xixi Yan; Yingyi Liao; Deguan Yu; Congcong Wen; Zheng Xiang
D-pinitol could be a potential therapeutic agent for the treatment of diabetes mellitus (DM) type II. In this work, a sensitive and rapid ultra performance liquid chromatography coupled with tandem mass spectrometry method was firstly developed and validated for the determination and pharmacokinetic study of D-pinitol in rat plasma. D-pinitol and 5,7-dihydroxychromone (Internal Standard, IS) were completely
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Pipette-tip micro-solid phase extraction based on melamine-foam@polydopamine followed by ultra-high-performance liquid chromatography–quadrupole–time-of-flight mass spectrometry for detection of psychotropic drugs in human serum J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-31 Xiaodan He; Tangqiang Sun; Ling Wang; Xuehua Jiang
In this work, pipette-tip micro-solid phase extraction (PT-μSPE) which packed by melamine-foam@polydopamine (MF@PDA) coupled with ultra-high-performance liquid chromatography–quadrupole–time-of-flight mass spectrometry (UHPLC-QTOF) was developed for extraction and determination of psychotropic drugs in serum samples. Considering the operation back pressure, the melamine-foam as carrier material with
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Quantitative determination of primaquine-5,6-ortho-quinone and carboxyprimaquine-5,6-ortho-quinone in human erythrocytes by UHPLC-MS/MS J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-30 Washim Khan; Yan-Hong Wang; N.P. Dhammika Nanayakkara; H.M.T. Bandara Herath; Zachara Catchings; Shabana Khan; Pius S. Fasinu; Mahmoud A. ElSohly; James D. McChesney; Ikhlas A. Khan; Narayan D. Chaurasiya; Babu L. Tekwani; Larry A. Walker
The antimalarial drug primaquine (PQ) causes methemoglobinemia and hemolysis in individuals with a genetic deficiency of glucose 6-phosphate dehydrogenase. Reactive oxygen species (ROS) generated by redox cycling of the metabolite primaquine-5,6-orthoquinone (POQ) in erythrocytes has been attributed to be responsible for the toxicity of PQ. Carboxyprimaquine (CPQ), the major human plasma metabolite
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A sensitive HPLC-FLD method for the quantification of alpelisib, a novel phosphatidylinositol 3-kinase inhibitor, in rat plasma: Drug metabolism and pharmacokinetic evaluation in vitro and in vivo J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-19 Seong-Wook Seo; Ji-Min Kim; Dong-Gyun Han; Dongho Geum; Hwayoung Yun; In-Soo Yoon
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Simultaneous determination of five amino acid neurotransmitters in rat and porcine blood and brain by two-dimensional liquid chromatography J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-19 Xue-Jiao Zhao; Na Wang; Ming-Jun Zhang; Sha-Sha Liu; Hui Yu; Mo-Huan Tang; Zhao-Ying Liu
A method for the simultaneous determination of aspartic acid (Asp), glutamic acid (Glu), glycine (Gly), taurine (Tau) and gamma-aminobutyric acid (GABA) in animal blood and brain by two-dimensional liquid chromatography (2D-LC) combined with ultraviolet detection was established for the first time. First, the amino acid neurotransmitters (AANTs) were labeled on the corresponding fluorescent derivatives
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Use of an alternative signature peptide during development of a LC-MS/MS assay of plasma nivolumab levels applicable for multiple species J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-08 Mayu Ohuchi; Shigehiro Yagishita; Kazuaki Taguchi; Yasushi Goto; Masaru Fukahori; Yuki Enoki; Takashi Shimada; Masakazu Yamaguchi; Kazuaki Matsumoto; Akinobu Hamada
Recently, immune checkpoint inhibitors, including anti-programmed cell death protein 1 (PD-1) antibodies, have dramatically changed treatment strategies for several cancers. In pharmacokinetic/pharmacodynamic studies, experiments using a variety of animal species are assumed. We have identified optimal multiple reaction monitoring transitions for signature candidate peptides of nivolumab in human,
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A fast and simple method for the simultaneous analysis of midazolam, 1-hydroxymidazolam, 4-hydroxymidazolam and 1-hydroxymidazolam glucuronide in human serum, plasma and urine J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-03 A. Mireille A. Wessels; Mathieu S. Bolhuis; Wouter Bult; Maarten W.N. Nijsten; Martin C.J. Kneyber; Daan J. Touw
For the quantification of the sedative and anesthetic drug midazolam and its main (active) metabolites 1-hydroxymidazolam, 4-hydroxymidazolam and 1-hydroxymidazolam glucuronide in human serum, human EDTA plasma, human heparin plasma and human urine a single accurate method by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) has been developed. Protein precipitation
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Recent advances in the analysis of human milk oligosaccharides by liquid phase separation methods J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-16 Felicia Auer; Gabor Jarvas; Andras Guttman
Human milk is a complex, dynamically changing biological fluid, which contains a large amount of non-conjugated carbohydrates, referred to as human milk oligosaccharides (HMOs). These HMOs are very important for the infants as they play important roles in the formation of the gut microbiome, the immune system and support brain development. HMOs show highly complex structural diversity due to numerous
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Selective recognition of nucleosides by boronate affinity organic-inorganic hybrid monolithic column J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-07 Sisem Ektirici; Ilgım Göktürk; Fatma Yılmaz; Adil Denizli
Boronic acids are important ligands used to selectively recognize and enrich compounds containing cis-diol groups such as nucleosides. In the present study, organic-inorganic hybrid [POSS-MAH-BPA] monolithic column was prepared for the first time in the literature as a new boronate affinity system for the recognition of nucleosides. The selectivity of the [POSS-MAH-PBA] boronate affinity monolithic
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Development and validation of a UHPLC-ESI-QTOF mass spectrometry method to analyze opines, plant biomarkers of crown gall or hairy root diseases J. Chromatogr. B (IF 3.004) Pub Date : 2020-11-26 Rosa Padilla; Vincent Gaillard; Thao Nhi Le; Floriant Bellvert; David Chapulliot; Xavier Nesme; Yves Dessaux; Ludovic Vial; Céline Lavire; Isabelle Kerzaon
Opines are low-molecular-weight metabolites specifically biosynthesized by agrobacteria-transformed plant cells when plants are struck by crown gall and hairy root diseases, which cause uncontrolled tissue overgrowth. Transferred DNA is sustainably incorporated into the genomes of the transformed plant cells, so that opines constitute a persistent biomarker of plant infection by pathogenic agrobacteria
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Fast and effective chromatographic separation of polymersomes from proteins by multimodal chromatography J. Chromatogr. B (IF 3.004) Pub Date : 2020-11-28 Michael Mertz; Florian Golombek; Susanne Boye; Silvia Moreno; Kathrin Castiglione
Artificial vesicles made of block copolymers, so-called polymersomes, represent a versatile chassis for the creation of functionalized nanocompartments with a wide range of biotechnological applications. The specific application depends on the biomolecules – usually proteins – that are positioned in the interior, in the membrane or on the surface of the vesicles. However, not all added proteins are
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Detailed analysis of the fatty acid composition of six plant-pathogenic bacteria J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-03 Nina Wiedmaier-Czerny; Dorothee Schroth; Shiri Topman-Rakover; Aya Brill; Saul Burdman; Zvi Hayouka; Walter Vetter
Bacteria show distinct and characteristic fatty acid (FA) patterns which can be modified by environmental conditions. In this study, we cultivated six plant-pathogenic bacteria of agricultural concern and performed a detailed analysis of the fatty acid composition. The study covered four strains of the gram-negative Xanthomonas campestris pathovar (pv) campestris (Xcc), Xanthomonas perforans (Xp),
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Development and validation of a UPLC–MS/MS method for quantification of C-005, a novel third-generation EGFR TKI, and its major metabolite in plasma: Application to its first-in-patient study J. Chromatogr. B (IF 3.004) Pub Date : 2020-12-07 Zhenlei Wang; Wei Ye; Yongping Qin; Haitao You; Shuai Zhang; Feng Fan; Yongsheng Wang; Li Zheng
C-005 is a novel third-generation EGFR tyrosine kinase inhibitor for the treatment of non-small cell lung cancer (NSCLC). To support its clinical trial, we developed a rapid and sensitive bioanalytical method based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) technique for the quantification of C-005 and its major metabolite in NSCLC patients following international
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