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Quantitative Determination of Fosfomycin in 10 μL of Plasma and Dialysate by Hydrophilic Interaction Liquid Chromatography Electrospray Ionization Mass Spectrometry J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-11 Shopova T, Hüppe T, Wolf B, et al.
AbstractFosfomycin is an antibiotic with a broad spectrum of activity against many multidrug-resistant bacterial strains. It is mainly excreted unchanged by the kidneys, and its half-life therefore depends on kidney function which varies considerably among individuals, and within individuals over time. Proper fosfomycin dosing thus depends on assaying blood concentration of the drug. We developed and
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Stability-Indicating Method Development and Validation for Busulfan Drug Substance by Gas Chromatography-Flame Ionization Detector J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-09 Khan A, Winter G, Tan S.
AbstractA new, simple and stability-indicating gas chromatography-flame ionization detection (GC-FID) method was developed and validated for the quantitative determination of busulfan and its organic impurities (OI) in drug substance without derivatization. The chromatographic attributes were achieved on a fused silica capillary column (0.53 mm × 30 m, 1.0 μm, USP Phase G42), using hydrogen as a carrier
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HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-03 Yang X, Liu Y, Chen T, et al.
AbstractSeparation of natural compounds directly from the crude extract is a challenging work for traditional column chromatography. In the present study, an efficient method for separation of three main compounds from the crude extract of Dracocephalum tanguticum has been successfully established by high-speed counter-current chromatography (HSCCC). The crude extract was directly introduced into HSCCC
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Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-24 Debbabi H, El Mokni R, Jlassi I, et al.
AbstractChemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds
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Simultaneous Determination of Paracetamol, Propyphenazone and Caffeine in Presence of Paracetamol Impurities Using Dual-Mode Gradient HPLC and TLC Densitometry Methods J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-21 Ibrahim H, Hamdy A, Merey H, et al.
AbstractTwo chromatographic methods were validated for the determination of the widely prescribed analgesic and antipyretic drug combination of paracetamol (PC) (recently integrated into the supportive treatment of COVID-19), propyphenazone (PZ) and caffeine (CF) in the presence of two PC impurities, namely 4-aminophenol and 4-nitrophenol. A “dual-mode” gradient high-performance liquid chromatography
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Andrographolide: A Review of Analytical Methods J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-21 Patil R, Jain V.
AbstractBicyclic diterpenoid lactone andrographolide is regarded as a “natural antibiotic” as it is known to exhibit a range of bioactivities including anti-inflammatory, antibacterial, antipyretic, antineoplastic, cardioprotective, hepatoprotective and hypoglycaemic, and is present in Andrographis paniculata. The aim of this article is to review the information on analytical methods for andrographolide
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Stability-Indicating RP-HPLC Method for the Estimation of Process-Related Impurities and Degradation Products in Fesoterodine Fumarate by Using a Mass Spectrometric Compatible Mobile Phase J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-14 Kumar N, Sangeetha D, Kalyanraman L.
AbstractThe objective of the present work was to develop a sensitive, selective, accurate and precise stability-indicating HPLC method for quantification of degradation products and process-related impurities in fesoterodine fumarate extended-release tablets. The degradation profile was studied by conducting forced degradation studies and all the degradation products formed during degradation were
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Determination of PAH Benzo[a]anthracene, Chrysene, Benzo[b]flouranthene and Benzo[a]pyrene in Hydro Alcoholic Herbal Extracts with Fluorescence Detector Using Solid-Phase Extraction J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-14 Benny A, Benny A, Aravind E.
AbstractAn appropriate purification and quantification method has been developed for polycyclic aromatic hydrocarbons (PAH) in hydro alcoholic herbal extracts. For this, Bacopa monnieri, Camellia sinensis, Withania somnifera and Andrographis paniculata samples were extracted with modified solid-phase extraction (SPE) and the PAH were quantified using liquid chromatography coupled to fluorescence detector
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Ultra-trace Extraction of Two Bactericides Via Ultrasound-Assisted Dispersive Liquid-Liquid Microextraction J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-11 Gao X, Si X, Yuan Y, et al.
Abstract BackgroundA simple, rapid and sensitive method coupling ultrasound-assisted dispersive liquid-liquid microextraction (DLLME) with ultra-high performance liquid chromatography-tandem mass spectrometry was developed for the simultaneous determination of malachite green (MG) and crystal violet (CV) in different water samples. ObjectiveIn ultrasound-assisted DLLME procedure, several parameters
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Quantitative Polyunsaturated Fatty Acid Analysis of Chia Seed Oil by High-Performance Liquid Chromatography J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-10 Liu B, Yan W.
AbstractAlpha-linolenic acid (ALA) and linoleic acid (LA), abundant in chia seed oil, are useful polyunsaturated fatty acids (PUFA) with numerous health benefits. The objectives of the present study were to explore the optimum extraction condition of chia seed oil and the possibilities of direct analysis of ALA and LA in chia seed oil by reversed-phase high-performance liquid chromatography with ultraviolet
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Systematic Development and Validation of a RP-HPLC Method for Estimation of Abiraterone Acetate and its Degradation Products J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-10 Beg S, Malik A, Afzal O, et al.
AbstractThe present study described the development of a reversed-phase liquid chromatographic method for the estimation of abiraterone acetate by Quality by Design (QbD) approach. Using an isocratic solvent system for the mobile phase, the chromatographic estimation of analyte was performed on a Hypersil BDS C18 column using mobile phase mixture containing acetonitrile and water with pH adjusted with
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Stability Indicating Method for Polysorbate 80 in Protein Formulations J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-26 Gregory K Webster; Jean C Chang; Julie L Heflin
Polysorbates (also known as “Tween”) are common components of protein formulations used to minimize protein adsorption and stabilize the protein. These nonionic surfactants are heterogenous mixtures of fatty acids with a complex reversed-phase profile due to the inhomogeneity of the polymers present. Polysorbates can be oxidized, which can be hard to detect in the complex polymer profile. Further adding
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Rapid High-Performance Liquid Chromatography Method for Levodopa Quantitation at Low UV Wavelength: Application of Pharmacokinetics Study in Rat Following Intranasal Delivery J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-26 Elahehnaz Parhizkar; Zahra Mohammadi; Shohreh Alipour
Levodopa is widely administered orally in clinical treatment of Parkinson’s disease; however, due to levodopa various oral absorption and low bioavailability, intranasal delivery seems to be a suitable alternative route of administration. Pluronic F-127 is a thermosensitive polymer, which can form gel at nasal cavity temperature and increase drug residence time. In this study, a rapid High Performance
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Application of Quality Risk Assessment and DoE-Based Enhanced Analytical Quality by Design Approach to Development of Chromatography Method for Estimation of Combined Pharmaceutical Dosage Form of Five Drugs J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-23 Pintu B Prajapati; Kajal Jayswal; Shailesh A Shah
The high-performance liquid chromatography method was only reported for simultaneous estimation of aspirin, simvastatin, ramipril, atenolol and hydrochlorothiazide in polycap capsule. High-performance thin-layer chromatography (HPTLC) method is now accepted as a method of analysis by many pharmaceutical industries and included as an official method in monographs of pharmacopeias of many countries.
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HPLC Detection and Antioxidant Capacity Determination of Brown, Red and Green Algal Pigments in Seaweed Extracts J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-14 Sibel Yalçın; Özge Karakaş; Emine Şükran Okudan; Kevser Sözgen Başkan; Sema Demirci Çekiç; Reşat Apak
This study was carried out to determine the main pigments in some different selected seaweeds and to reveal their antioxidant potential regarding the ever-increasing demand for utilization of marine pigments in human health and nutrition. The individual amounts of algal pigments were found by reverse phase high-performance liquid chromatography (HPLC) and their total antioxidant capacities (TAC) by
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A Sensitive and Specific ESI-Positive LC–MS/MS Method for the Quantitation of Estrogens in Human Serum in Under Three Minutes J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-12 Aaron Stella; Subhakar Dey
Amplifex Diene reagent was employed to derivatize estradiol (E2) to enhance the analyte signal at low picogram concentrations. This derivatization enabled measurement of E2 (and other estrogens) in ESI+ mode, earlier retention times for analytes than other methods, avoidance of MS harmful ammonium fluoride in mobile phases, and an LLOQ below 1 pg/mL. The sample preparation workflow involved liquid–liquid
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Separation of Fatty Acid Dimethyl Esters on an Ionic Liquid Gas Chromatographic Column J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-05 R E Pauls; B Pease
An ionic liquid (IL) 111 column was compared with other commonly employed stationary phases including polydimethyl siloxane and polyethylene glycol for the separation of fatty acid monomethyl and dimethyl esters. The fatty acid esters employed in this study were derived from metathesis reactions of vegetable oils both with and without olefins. The IL 111 column demonstrated enhanced performance compared
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Vortex-Assisted Dispersive Liquid–Liquid Microextraction Coupled with Deproteinization for Determination of Nateglinide in Human Plasma Using HPLC/UV J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-05 Mohamed A Hammad; Amira H Kamal; Reham E Kannouma; Fotouh R Mansour
A validated method for preconcentration and determination of nateglinide in plasma was developed using vortex-assisted dispersive liquid–liquid microextraction. Different variables that affect extraction efficiency were studied and optimized, including type and volume of extractant, type and volume of disperser, pH of diluent, salt addition effect, centrifugation and vortex time. Nateglinide was extracted
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Determination of Trace Bromate in Drinking Water with High Chloride Matrix by Cyclic Ion Chromatography J Chromatogr Sci (IF 1.28) Pub Date : 2020-12-05 Mingli Ye; Pavel N Nesterenko; Ying Lu; Xueting Huang; Xiaoling Jin; Meilan Chen
An ion chromatography (IC) system with switching recycling column was developed for the determination of bromate in drinking water with high chloride content. The system included a pump, two switching valves, guard column and analytical column, suppressor and a conductivity detector. In this method, the single eluent was used for both online collection and matrix elimination, and bromate was eluted
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Isolation and Purification of Five Phloroglucinol Derivatives From Agrimonia Pilosa Ledeb by Counter-Current Chromatography Using Non-Aqueous Solvent Systems J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-30 Lulu Fu; Chenghua Lu; Shengqiang Tong; Min Xu; Lan Tang; Weiguang Shan
Five non-polar phloroglucinol derivatives, viz. pseudo-aspidin, α-kosin and agripinol A-C were isolated and purified from Agrimonia pilosa Ledeb by semi-preparative counter-current chromatography. The separation was performed by a two-step elution with non-aqueous solvent systems. In the first step, an elution mode of a two-phase solvent system consisting of n-hexane-acetonitrile-dichloromethane-methanol
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Determination of Potential Genotoxic Impurity, 5-Amino-2-Chloropyridine, in Active Pharmaceutical Ingredient Using the HPLC-UV System J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-25 Murat Soyseven; Rüstem Keçili; Hassan Y Aboul-Enein; Göksel Arli
A novel analytical method, based on high-performance liquid chromatography with a UV (HPLC-UV) detection system for the sensitive detection of a genotoxic impurity (GTI) 5-amino-2-chloropyridine (5A2Cl) in a model active pharmaceutical ingredient (API) tenoxicam (TNX), has been developed and validated. The HPLC-UV method was used for the determination of GTI 5A2Cl in API TNX. The compounds were separated
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A Screening Method for Cyanide in Blood by Dimethoxytriazinyl Derivatization-GC/MS J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-05 Akinori Yamaguchi; Hajime Miyaguchi
A simple screening analysis of cyanide in blood has been developed, using 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM). DMTMM, a convenient reagent for dehydrocondensation, converted cyanide to 2-cyano-4,6-dimethoxy-1,3,5-triazine, the dimethoxytriazinyl derivative of cyanide. This reaction proceeded in whole blood samples after treatment with trichloroacetic acid, and
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Chromatographic Separation of Fluoroquinolone Drugs and Drug Degradation Profile Monitoring through Quality-by-Design Concept J Chromatogr Sci (IF 1.28) Pub Date : 2020-10-22 Satya Prasad Asu; Naveen Kumar Sompalli; Akhila Maheswari Mohan; Prabhakaran Deivasigamani
The article reports on the development of an efficient, robust and sensitive HPLC-DAD method for the simultaneous determination of five fluoroquinolone-based antimicrobial drugs, namely ciprofloxacin, moxifloxacin, norfloxacin, ofloxacin and pefloxacin in both aquatic and tablet formulations. The robustness of the high-performance liquid chromatography with diode-array detection (HPLC-DAD) method has
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Magnetic Polydopamine Modified with Choline-Based Deep Eutectic Solvent for the Magnetic Solid-Phase Extraction of Sulfonylurea Herbicides in Water Samples J Chromatogr Sci (IF 1.28) Pub Date : 2020-10-20 Wang D, Lu Z, Guan X, et al.
AbstractA novel magnetic solid-phase extraction technique coupled to ultraperformance liquid chromatography has been developed for separation and preconcentration of four sulfonylurea herbicides (sulfosulfuron, bensulfuron-methyl, pyrazosulfuron-ethyl and halosulfuro-methyl) in aqueous samples. The key point of this method was the application of a novel magnetic nanomaterial that composed of a low
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Sensitive and Fast Determination of Ceftiofur in Honey and Veterinary Formulation by HPLC-UV Method with Pre-column Derivatization J Chromatogr Sci (IF 1.28) Pub Date : 2020-10-20 Noha Rashed; Sahar Zayed; Fatma Fouad; Amany Abdelazeem
A novel, sensitive and rapid high performance liquid chromatography (HPLC) method for the determination of ceftiofur by pre-column derivatization with 1,2-naphthoquinone-4-sulfonate. Analysis was performed within 5 min on a Kinetex C18 column based on core-shell technology. The mobile phase composed of acetonitrile-water (50:50, v/v) pumped isocratically at a flow rate of 1.0 mL/min under UV detection
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Simultaneous Measurement of Sex Steroid Hormones in Largemouth Bass (Micropterus salmoides) Plasma by Application of Liquid Chromatography–Tandem Mass Spectrometry J Chromatogr Sci (IF 1.28) Pub Date : 2020-10-20 Viet D Dang
Sex steroid hormones are potential biomarkers of reproductive function in teleost fish, but their measurement continues to rely on antibody-based assays. The objective of this study was to optimize a robust and simultaneous liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for measurement of eight steroid hormones (cortisol, 11-ketotestosterone, estradiol, 17α-ethynyl estradiol, estrone
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Untargeted SPME–GC–MS Characterization of VOCs Released from Spray Paint J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-05 Tiessen M, Stock N, Stotesbury T.
AbstractPaints are a common form of physical evidence encountered at crime scenes. This research presents an optimized method for the untargeted analysis of volatile organic compounds (VOCs) in spray paint using solid-phase microextraction–gas chromatography–mass spectrometry (SPME–GC–MS). The presence and persistence of VOCs were monitored in 30 minute intervals, over a 4 hour period, in a triplicate
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Development and Validation of Stability Indicating High-Performance Liquid Chromatographic Method for Determination of Cyproheptadine Hydrochloride, its Impurity And Degradation Product J Chromatogr Sci (IF 1.28) Pub Date : 2020-11-02 Abdelrahman M, Abdelaleem E, Ali N, et al.
AbstractThis work presents a sensitive, accurate and selective RP-HPLC method for simultaneous determination of cyproheptadine HCl (CPH), its impurity B (dibenzosuberone) and CPH oxidative degradation product (10,11-dihydroxy-dibenzosuberone) in bulk powder and in pharmaceutical formulation. The RP-HPLC method depends on isocratic elution using C8 column and mobile phase consisting of 0.05 M KH2PO4
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Systematic Error for Extraction of Controlled Substances from Plant/Fungal Materials. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-18 Júlia Nagy,Tibor Veress
AbstractThe aim of this work was to investigate the applicability of a mathematical model developed for the description of supercritical fluid extraction (SFE) of cannabinoids from marijuana and hashish for liquid extraction of other substances. The mentioned model is applicable for dynamic SFE whose implementation is analogous to liquid–solid extraction in quasi-counter current mode. According to
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Impurity Profiling of a Novel Anti-MRSA Antibacterial Drug: Alalevonadifloxacin. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-15 Kiran R Patil,Vipul P Rane,Vinod K Ahirrao,Ravindra D Yeole
AbstractAlalevonadifloxacin (ALA, formerly described as WCK 2349) is a novel antibacterial drug developed to treat infections caused by Gram-positive bacteria. The current study was aimed to identify and quantify impurities in ALA. Mass spectrometry (MS) compatible reverse phase liquid chromatographic method was developed to identify the impurities in ALA. Three impurities were identified based on
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Novel Eco-friendly HPLC Methods Using Refractive Index Detector for Analysis of Three Veterinary Antibiotics in Pharmaceutical Formulations and Rat Plasma. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-08 Mohammed Gamal,Lobna Mohammed Abd Elhalim
AbstractAntibiotic resistance increases the human mortality rate nowadays. The main purpose of the present study was to develop green reversed-phase high-performance liquid chromatography (RP-HPLC) methods with a refractive index detector for the assay of the three veterinary antibiotics (VAs), i.e., maduramicin ammonium (MA), apramycin sulfate (AS) and clarithromycin (CLA) in pharmaceutical dosage
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Advances in Separation and Purification of Bioactive Polysaccharides through High-speed Counter-Current Chromatography. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-08 Yu Yang,Bilal Muhammad Khan,Xiping Zhang,Yongjie Zhao,Kit-Leong Cheong,Yang Liu
Polysaccharides, with an extensive distribution in natural products, represent a group of natural bioactive substances having widespread applications in health-care food products and as biomaterials. Devising an efficient system for the separation and purification of polysaccharides from natural sources, hence, is of utmost importance in the widespread applicability and feasibility of research for
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Clarification on "Critical Comments on 'Assessment of the Thermodynamic Properties of DL-p-Mentha-1,8-diene, 4-isopropyl-1-methylcyclohexene (DL-Limonene) by Inverse Gas Chromatography (IGC)'". J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-07 Negin Farshci,Ali Abbasian
A clarification is given regarding recent polemic over our original published study. The author of this polemic has claimed a few issues about the computations and data listed in our work such as choosing the wrong temperature unit and the incorrect calculation of molar heat of vaporization. Here it is elaborated the complete data and computations that are not included in the original paper, and list
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Dispersive Liquid-Liquid Microextraction Combined with Microwave Demulsification for Determination of FAME Residuals in Biodiesel Wastewater. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-05 Guo-Wei Yu,Xia-Jun Wang,Peng Wang,Yi-Ping Zhao,Jing Nie,Zu-Guang Li,Xiu-Gui Fang,Maw-Rong Lee
AbstractBiodiesel consists of various fatty acid methyl esters (FAMEs) that are mainly produced through transesterification of plant oil or animal fat. It is essential for biodiesel to be purified utmostly to meet its product standard before being traded, while the universal purification method has been water washing. However, water washing inevitably causes the residual of FAMEs in wastewater, which
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Simultaneous Determination of Three Coumarins in Rat Plasma by HPLC-MS/MS for Pharmacokinetic Studies Following Oral Administration of Chimonanthi Radix Extract. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-05 Jing Zhang,Quan Wen,Meng-Ying Zhou,Chen-Cong Zhong,Yulin Feng,Ting Tan
AbstractChimonanthi Radix (CR) is widely used in the treatment of influenza in China. Extensive studies revealed that the major bioactive constituents of CR were coumarins. However, pharmacokinetic study of coumarins in CR has not been fully studied. The purpose of this study was to establish a convenient and effective high-performance liquid chromatography–tandem mass spectrometry method that was
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Quantification of the Plasma Concentrations of Perampanel Using High-Performance Liquid Chromatography and Effects of the CYP3A4*1G Polymorphism in Japanese Patients. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-05 Sho Ohkubo,Yumiko Akamine,Tadashi Ohkubo,Yuka Kikuchi,Masatomo Miura
AbstractHere, we developed a novel high-performance liquid chromatography (HPLC) method for quantification of perampanel in clinical practice and investigated the relationships between the plasma concentrations of perampanel obtained by this HPLC method and the CYP3A4*1G polymorphism. The developed HPLC method was validated based on US Food and Drug Administration. The developed HPLC method could be
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Failure Mode Critical Effect Analysis and Design of Experiment-Based Robust Chromatographic Method for Simultaneous Estimation Lornoxicam and Eperisone Hydrochloride. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-05 Pintu Prajapati,Vipul Radadiya,Shailesh Shah
AbstractFailure mode critical effect analysis and design of experiment-based high performance thin layer chromatography (HPTLC) method has been developed for simultaneous estimation of lornoxicam (LOC) and eperisone hydrochloride (EPR). Failure modes were identified on the basis of prior knowledge and experimental data with the help of Ishikawa diagram for the development of method. The criticality
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Traditional Horticulture Practices Increase the Production of Selected Withanolides in Withania Somnifera (L.) Dunal-A RP-UFLC Analysis. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-02 Gireesh M Ankad,Sandeep R Pai,Jagadishchandra Hiremath,Harsha V Hegde
AbstractThe study evaluates the effect of two traditional horticulture treatments mentioned in Vrikshayurveda, a text from ancient India on the science of plant life, namely Kunapa jala (KJ) and Pancha gavya (PG) on the production of Withaferin A (WFA), withanolide A (WIA) and Withanolide B (WIB) in Withania somnifera (L) Dunal. Leaves and roots of W. somnifera were collected from different treated
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Cross Referencing 2D-LC Determination of Intact Gliptins in Urine. J Chromatogr Sci (IF 1.28) Pub Date : 2020-09-01 Amal M Mohamad,Cenk A Andac,Sena Caglar Andac
AbstractDipeptidyl peptidase-4 inhibitors, so-called gliptins, constitute a fairly novel class of oral hypoglycemic agents. The development and validation of an automated online SPE-LC-UV method to determine intact sitagliptin, saxagliptin, vildagliptin and metformin simultaneously in human urine samples were performed. For the two-dimensional chromatographic separation, a Gemini C18 (250.0 × 4.6 mm
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Combined Use of Cu(II)-L-Histidine Complex and β-Cyclodextrin for the Enantioseparation of Three Amino Acids by CE and a Study of the Synergistic Effect. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-31 Zifu Xu,Jin Guan,Huili Shao,Shitong Fan,Xiaoyu Li,Shuang Shi,Feng Yan
AbstractA new capillary electrophoresis method was applied to chiral separation of three amino acids, including D,L-tryptophan, D,L-tyrosine and D,L-phenylalanine. The chiral resolution was attained in an untreated fused-sillica capillary using a dual chiral selector, which was made up of Cu(II)-L-histidine complex and β-cyclodextrin (CD). The cardinal factors influencing its separation efficiency
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On-Line Sorbentless Cryogenic Needle Trap and GC-FID Method for the Extraction and Analysis of Trace Volatile Organic Compounds from Soil Samples. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-26 Djavanshir Djozan,Jamal Norouzi,Mir Ali Farajzadeh
AbstractIn this study, an automated sorbentless cryogenic needle trap device (ASCNTD) coupled with a gas chromatograph (GC) was developed with the aim of sampling, pre-concentration and determination of volatile organic compounds (VOCs) from soil sample. This paper describes optimization of relevant parameters, performance evaluation and an illustrative application of ASCNTD. The ASCNTD system consists
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Rapid MSPD-LC-MS/MS Procedure for Determination of Pesticides in Potato Tubers. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-31 Magdalena Danek,Marcin Sajdak,Joanna Płonka,Hanna Barchańska
The program of potato protection recommended by the producers of agrochemicals requires application: thiamethoxam, lambda-cyhalothrin, deltamethrin, rimsulfuron and metalaxyl. Therefore, there is a risk that these pesticides are present in tubers, thus posing a toxicological risk to the consumer. In this respect, it is necessary to monitor the presence of these compounds in edible plants. Therefore
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Offline Selective Extraction Combined with Online Enrichment for Sensitive Analysis of Chondroitin Sulfate by Capillary Electrophoresis. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-31 Jie Gong,Guanglian Zhou,Yuanhong Wu,Siying Zhang,Xiumei Liu
A capillary electrophoresis (CE) method combined with online and offline enrichment for improving the detection sensitivity of chondroitin sulfate (CS) is established. The online enrichment method is based on the field-amplified sample stacking and large volume electrokinetic injection, and offline enrichment is based on the association between cetyltrimethylammonium chloride and CS. Experimental parameters
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Preparative Separation and Purification of Liquiritin and Glycyrrhizic Acid from Glycyrrhiza uralensis Fisch by High-Speed Countercurrent Chromatography. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-31 Hao Wang,Hu Shan,Haitao Lü
The technique of high-speed countercurrent chromatography (HSCCC) was applied to the preparative isolation and purification of liquiritin and glycyrrhizic acid from a crude extract of Glycyrrhiza uralensis Fisch for the first time. Using single factor and orthogonal design experiments, the best extraction conditions were 70% ethanol, 1:25 ratio of solid-to-liquid (w/v) and extracted 1.5 h at 80°C.
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Quality by Design Approach to Develop Stability Indicating Method to Quantify Related Substances and Degradation Products of Sacubitril by High Performance Liquid Chromatography. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-26 Sivaganesh Bommi,Subbalakshmi Jayanty,Satyanarayana Raju Tirumalaraju,Sivasankar Bandaru
Sacubitril (SBT) is a neprilysin inhibitor, approved by food and drug administration (FDA) in 2015, under the FDA’s priority review process. In this work, we report the validated stability indicating method of SBT by employing quality by design (QbD) principles related to analytical method development, capable in separation of 11 impurities. Chromatographic separation was performed on an ascentis phenyl
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Bioanalysis and Quadrupole-Time of Flight-Mass Spectrometry Driven In Vitro Metabolite Profiling of a New Boronic Acid-Based Anticancer Molecule. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-25 Aarati Dilip Zagade,Amit Shard,Shital Shinde,Amit Kumar Sahu,Pinaki Sengupta
(E/Z)-(4-(3-(2-((4-chlorophenyl)amino)-4-(dimethylamino)thiazol-5-yl)-2-(ethoxy carbonyl)-3-oxoprop-1-en-1-yl)phenyl) boronic acid, a newly developed molecule having anticancer activity serves as a potential candidate for the further drug development process. In this study, to ascertain the anticancer potential of the molecule, we screened it against different cell lines and compared the activity against
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Qualitative and Quantitative Analysis of the New Sulfone Bactericide 2-(4-Fluorophenyl)-5-(Methylsulfonyl)-1,3,4-Oxadiazole and Identification of Its Degradation Pathways in Paddy Water. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-22 Xingang Meng,Niao Wang,Xiaofang Long,Lingzhu Chen,Deyu Hu
Rapid and simple methods for the determination of Jiahuangxianjunzuo (JHXJZ) in paddy water, brown rice, soil and rice straw was developed and validated. This method involved the use of ultrahigh-performance liquid chromatography equipped with photodiode array detector. The most important factor was chromatographic conditions, as identified through an orthogonal experimental design. This method showed
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Identification of the Metabolites in Rat Urine after Oral Administration and Elucidation of the Metabolic Process of Naozhenning Granule Using LC-MS. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-18 Rongrong Li,Yanzhi Liu,Haixia Liu,Chunhong Wei,Jiang Wang,Xuemei Qin,Xuwen Wang,Zhenyu Li
Naozhenning (NZN) granule, a Chinese herbal formula, is widely used to treat craniocerebral trauma and promote functional recovery. In our previous study, the chemical components, as well as the serum metabolites in the male Sprague–Dawley rats of the NZN granule after oral administration were characterized. In this study, the urine metabolites in the male Sprague–Dawley rats were further investigated
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An Effective Acid-Base-Induced Liquid-Liquid Microextraction Based on Deep Eutectic Solvents for Determination of Testosterone and Methyltestosterone in Milk. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-14 Xiao Li,Tao Yuan,Ting Zhao,Xiaomei Wu,Yaling Yang
An environmentally friendly method for the determination of testosterone and methyltestosterone by acid–base-induced deep eutectic solvents liquid–liquid microextraction (DES-ABLLME) combining with high-performance liquid chromatography was established. The deep eutectic solvent (DES) consisting of menthol:lauric acid:decanoic acid (3:1:1) can act as both hydrogen bond donor and hydrogen bond acceptor
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Validated Liquid Chromatography-Tandem Mass Spectroscopic Method for Simultaneous Determination of Certain Drugs Used in Cataract Surgery in Rabbit Aqueous Humor. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-14 Mohammed W Nassar,Khalid A Attia,Ahmed A Mohamed,Ragab A Said,Ahmed El-Olemy,Mohamed A Hasan
A sensitive, selective, accurate and precise ultra-high performance liquid chromatography–Tandem mass spectrometry (MS/MS) method was developed and validated for the simultaneous determination of drugs used as eye drops in cataract surgery in aqueous humor. Cataract surgery requires a powerful mydriatic eye drops combination such as cyclopentolate hydrochloride and phenylephrine hydrochloride to dilate
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Online Liquid Microextraction Coupled with HPLC-ABTS for Rapid Screening of Natural Antioxidants: Case Study of Three Different Teas. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-13 Zheng-Ming Qian,Bo-Wen Fang,Hai-Ming Chen,Chun-Hong Li,Qi Huang,Ling Chen,Wen-Jia Li,De-Qiang Li
In the present study, an online liquid extraction coupled with high-performance liquid chromatography-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (HPLC-ABTS) system for rapid screening of antioxidants in tea samples was proposed. As an example, the tea samples were firstly extracted by online HPLC extractor with mobile phase at 70°C, then the hyphenated HPLC-ABTS was used for the chromatographic
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New and validated RP-HPLC Method for Quantification of Safinamide Mesylate in Presence of Its Basic Degradate, Levodopa and Ondansetron: Application to Human Plasma. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-10 Amira M El-Kosasy,Lobna A Hussein,Nesma M Mohamed,Nahla N Salama
A simple, precise, rapid and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for analysis of safinamide mesylate (SAF) in presence of its basic degradate, and co-administered drugs levodopa and ondansetron. The mobile phase consisted of acetonitrile and 20 mM potassium dihyrogen orthophosphate buffer having pH = 5 (40: 60 v/v). Quantification
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Application of Optimized and Validated Agar Overlay TLC-Bioautography Assay for Detecting the Antimicrobial Metabolites of Pharmaceutical Interest. J Chromatogr Sci (IF 1.28) Pub Date : 2020-08-07 Bettadapura Rameshgowda Nuthan,Devaraju Rakshith,Kuppuru Mallikarjunaiah Marulasiddaswamy,H C Yashavantha Rao,Kolathur Puttamadaiah Ramesha,Nagabhushana Chandra Mohana,Shiva Siddappa,Doreraj Darshan,Kigga Kaadappa Sampath Kumara,Sreedharamurthy Satish
The agar overlay TLC–bioautography is one of the crucial methods for simultaneous in situ detection and separation of antimicrobial metabolites of pharmaceutical interest. The main focus of this research relies on the dereplication of an antimicrobial metabolite coriloxin derived from mycoendophytic Xylaria sp. NBRTSB-20 with a validation of agar overlay TLC–bioautography technique. This polyketide
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Magnetic Graphene Dispersive Solid-Phase Extraction for the Determination of Phthalic Acid Esters in Flavoring Essences by Gas Chromatography Tandem Mass Spectrometry. J Chromatogr Sci (IF 1.28) Pub Date : 2020-07-29 Jing Zhu,Binglin Lu,Shanshan Liu,Aifei Xu,Guifang Tang,Zhiyan Chen,Yuling Pan,Gangling Tang,Fei Yang,Yun Zhou
In the present study, a sensitive, efficient and repeatable method for the simultaneous extraction and determination of 13 types of phthalic acid esters (PAEs) in flavoring essence samples using magnetic graphene solid-phase extraction coupled with gas chromatography tandem mass spectrometry was developed. Due to the unique structure of magnetic graphene, it has several advantages, such as large surface
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Polysaccharide-Based Chiral Stationary Phases on Gold Nanoparticles Modified Silica Beads for Liquid-Phase Separation of Enantiomers. J Chromatogr Sci (IF 1.28) Pub Date : 2020-07-28 Yuanyuan Li,Zhuxian Gao,Tong Chen,Manman Wei,Yulong Ma,Xiaoxia Ma,Hong Xu
Au nanoparticles (AuNPs) (10−15 nm in size) were prepared and deposited on the surfaces of silica particles functionalized using 3-aminopropyltriethoxysilane as the seeds under mild conditions. Then, Au seeds grew further and formed nanosheets by the method of gold chloride hydrate reduction. 3, 5-dimethylphenyl isocyanate derivative of cellulose as chiral selector was coated on the surfaces of SiO2/Au
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RP-HPLC Method for the Determination and Quantification of Artesunate. J Chromatogr Sci (IF 1.28) Pub Date : 2020-07-28 Muhammad Asad Saeed,Muhammad Tayyab Ansari,Bashir Ahmad Ch,Muhammad Zaman
A simple, rapid and cost-effective reverse phase high-performance liquid chromatographic (RP-HPLC) method was developed for the quantification of artesunate. C18 Promosil (ODS, 150 × 4.6 mm, 5 μm) column was used as stationary phase to separate the drug. Mobile phase comprised of ethanol: water (65:35) having pH 4.5 was run isocratically at a flow rate of 1 mL/min at 27°C. The method was validated
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Stability-Indicating RP-HPLC and CE Methods for Simultaneous Determination of Bisoprolol and Perindopril in Pharmaceutical Formulation: A Comparative Study. J Chromatogr Sci (IF 1.28) Pub Date : 2020-07-24 Said A Hassan,Nancy W Nashat,Mohamed R Elghobashy,Samah S Abbas,Azza A Moustafa
Two fast, accurate and selective stability-indicating methods were developed and validated for the simultaneous determination of bisoprolol, perindopril and three of their possible degradation products. The first proposed method was a gradient reversed phase-high-performance liquid chromatography (HPLC) method, whereas the second was a capillary electrophoresis method. The structures of the obtained
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UPLC-ESI/Q-TOF MS/MS Method for Determination of Vildagliptin and its Organic Impurities. J Chromatogr Sci (IF 1.28) Pub Date : 2020-07-24 Camila Ferrazza Alves Giordani,Sarah Campanharo,Nathalie Ribeiro Wingert,Lívia Maronesi Bueno,Joanna Wittckind Manoel,Cássia Virginia Garcia,Nadia Maria Volpato,Gabrielle Dineck Iop,Paola de Azevedo Mello,Erico Marlon de Moraes Flores,Elfrides Eva Scherman Schapoval,Martin Steppe
Vildagliptin (VLG) corresponds to a drug used for the treatment of diabetes mellitus. This disease requires continuous treatment, and so the control of impurities present in it is important to assure the quality of this drug. Thus, it is necessary to use sensitive and selective detection techniques and the ultra-performance liquid chromatography is a better option compared with high-performance liquid
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Maceration-Mediated Liquid-Liquid Extraction and Reverse-Phase High-Performance Liquid Chromatography-Based Pragmatic Analysis of Silybins. J Chromatogr Sci (IF 1.28) Pub Date : 2020-07-24 Syeda Mariam Hasany,Rahila Huma,Sumia Akram,Rizwan Ashraf,Muhammad Mushtaq
This study presents a pragmatic and easily scalable maceration-mediated liquid–liquid extraction (MMLLE) and reverse-phase high-performance liquid chromatography (RP-HPLC)-based determination of Silybins from plant material (Curcuma longa L.). The processing of calibration standards revealed that the RP-HPLC method was linear over a concentration range of 1–100 μg/mL with regression coefficient (R2) > 0
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Capillary Electrophoresis Method for Determination of Escitalopram Oxalate in Urine Samples and Different Dosage Forms. J Chromatogr Sci (IF 1.28) Pub Date : 2020-07-20 Wafa F S Badulla,Arın G Dal Poçan,Zeki Atkoşar,Göksel Arlı
Application of capillary electrophoresis (CE) has become a rapidly growing analytical technique for the estimation of drugs in pharmaceutical dosage forms and biological fluids. In this study, an effective and sensitive method was developed for the determination of escitalopram oxalate (ESC-OX) by CE with diode-array detection at 200 nm. The separation was achieved by a fused silica capillary with
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