Abstract The supercritical fluid chromatography technique is one of the most promising and innovative technologies used in the analysis as well as the separation of various racemates. The various enantiomers have been separated using SFC due to its several merits over conventional HPLC methods such as low cost, wide polarity compatibility, higher column efficiency, and fast analyzing capability, which

Abstract Fici Microcarpae Folium, the leaves of Ficus microcarpa, is commonly used for the treatment of influenza, chronic bronchitis, and tonsillitis in Traditional Chinese Medicine (TCM). Ficus microcarpa is mainly distributed in Guangdong and Guangxi provinces. The composition of herbal medicines can be easily affected by geographical regions, the quality control of FMF is essential. In this study

Abstract Simultaneous, rapid, and quantitative detection of multiple tumor markers is extremely important for the prevention and early diagnosis of cancers. In this study, we established a microfluidic-based indirect competitive chemiluminescence enzyme immunoassay for simultaneous detection of cancer antigen 15-3 (CA15-3) and progesterone receptor membrane components 1 (PGRMC1) for diagnosis of breast

Abstract Growing antibiotic resistance creates a need to find new antimicrobial agents characterized by diverse chemical structures and pharmaceutical activity. The higher plants synthesize many specialized metabolites as a part of their normal metabolic activity and have been extensively used for centuries in treatment of different diseases. They have a wide activity range depending on the species

Abstract Image retrieval techniques in combination with support vector machine (SVM) on smartphones were studied to make pattern recognition of planar chromatograms. A micellar TLC method employed less toxic and flammable solvents was studied to discriminate four herbal medicines (HMs) from citrus fruits. And the chromatograms were used to test the pattern recognition method. The TLC method employed

Abstract A fast, simple, and efficient sample pretreatment technique based on magnetic dodecyl functionalization of magnetic ferrite microspheres has been developed for the extraction and pre-concentration of phthalate esters from environmental water prior to high-performance liquid chromatography analysis. The magnetic composite microspheres were prepared using Fe3O4 microspheres as magnetic core

Abstract The 2,3,5,4’-tetrahydroxy-stilbene-2-O-β-D-glucoside (THSG) is a main active ingredient of Polygoni Multiflori Radix (PMR). In this study, THSG was found to be a differential chemical composition between PMR and its compatible herb pairs through chemometrics techniques. The effects of the herb pairs Codonopsis Radix-PMR, Astragali Radix-PMR, and Ophiopogonis Radix (OR)-PMR on the stability

Abstract Based on high-performance liquid chromatography-mass spectrometry (HPLC-MS) and reversed-phase high-performance liquid chromatography-mass spectrometry (RPLC-MS), the changes of drug metabolism and endogenous metabolites in normal rats and tumor model rats were studied by drug metabonomics. The changes of vital signs were used to evaluate the toxicity and pharmacological effects of the new

Abstract In this paper, a sensitive method for the determination of 3-chloro-1,2-propanediol (3-MCPD) by high performance liquid chromatography-fluorescence detection (HPLC-FLD) was established, in which 9-(2-Hydroxypropyl) adenine (HP-Ade) was selected as the fluorescent derivatization reagent because of its good water solubility and hydrophobicity. The principle of the method lies in the origination

Abstract Polysaccharide-based chiral stationary phases account for a majority of chiral HPLC separations. Their behavior in normal-phase and reversed-phase modes of separation has been studied extensively. However, much less is known about their behavior in polar-organic mode. In the course of our studies on preparative separation of various cyclic imides, lactams, and acetamides on Chiralcel OD, Chiralcel

Abstract Picroside-I, andrographolide and silybin exhibit manifold biological activities, present as bioactive components in Picrorhiza kurroa (roots), Andrographis paniculata (aerial parts) and Silybum marianum (seeds), respectively. A validated HPTLC method was developed for the simultaneous determination of picroside-I, andrographolide and silybin in the combined hydroalcoholic extract of the plants

Abstract Apocynum venetum is a kind of plant with high medicinal value. Its main component is rutin, which is widely used. In the present study, an efficient method based on high-speed counter-current chromatography (HSCCC) was established to separate rutin from A. venetum. The optimized solvent system composed of ethyl acetate-n-butanol-water (4:1:5, v/v) was selected for HSCCC separation. It was

Abstract Capreomycin (CMN) is a second-line aminoglycoside antibiotic for treating multi-drug resistant tuberculosis. Produced as a complex broth in microbial bioreactors, the downstream purification of CMN traditionally requires multiple steps. Current work aims to purify CMN as mixed isomers in a single step by preparative macroporous cation-exchange chromatography in >98% purity in >90% yield. Selection

Abstract A nonlinear lumped kinetic model of liquid chromatography is formulated and solved numerically to theoretically investigate the effect of column overloading on gradient elution. Linear solvent strength (LSS) model is utilized for Henry’s constant, non linearity coefficient and axial dispersion coefficient. A semi-discrete high-resolution finite volume scheme is extended and applied to obtain

Abstract Economical and fast analytical methods are required to determine the content of herbs and herbal medicine. With the increasing interest in rose tea and products prepared from rose flower extracts, it has emerged that products prepared from dried rose petals and rose flower extracts should have certain characteristics. In the European Pharmacopeia, there is no specific method to meet the specific

Abstract We present a video-densitometric high-performance thin-layer chromatography (HPTLC) quantification method for patulin in apple juice, developed in a vertical chamber from the starting point to a distance of 50 mm, using MTBE, n-pentane (9 + 5, v/v) as mobile phase. After separation the plate is sprayed with methyl-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) solution (40 mg

Abstract This study aims to determine a suitable artificial neural network (ANN) regression model for quantification of polyphenols from herbal matrices through thin-layer chromatography (TLC) images. Gallic acid was used as a standard for the development and evaluation of this analytical approach. Analyses were performed on samples extracted from the stem bark of Schinus terebinthifolius, an important

Abstract Acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) are considered as viable targets in the treatment of Alzheimer’s disease and other dementias. However, since clinically used AChE or dual AChE/BuChE inhibitors only provide some symptomatic treatment and lose their therapeutic effectiveness over time, in the last years an intensive search for multi-target compounds has started,

Abstract The field of planar chromatography has seen a resurgence due to interest in novel fabrication techniques for sorbent (stationary phase) layers. These novel sorbents must have high surface areas and achieve separations over short distances. Here, we present the first attempt to fabricate a planar chromatographic platform via laser ablation. Microchannels of 24 µm × 68 µm depth were produced

Abstract A combination of Ofloxacin, Tinidazole, Terbinafine hydrochloride, and Clobetasol propionate is used for treatment, control, prevention, and improvement of bacterial and fungal skin infections. Two chromatographic methods (HPLC and HPTLC) were developed for the quantitative determination of these four drugs in their combined topical formulation. The HPLC method employed a C18 (250 × 4.6 mm

Abstract In this paper, a discontinuous-Galerkin finite-element method (DG-FEM) is applied for the numerical solution of multi-component, non-equilibrium, lump kinetic model of liquid chromatography under nonlinear conditions. The model is analyzed for standard Bi-Langmuir type adsorption isotherms using Danckwert boundary conditions. Packed bed processes of liquid chromatography are modeled as convection-diffusion

Abstract In this study, we describe a dispersive liquid-liquid microextraction strategy for bongkrekic acid (BA) purification, enrichment, and liquid chromatography coupled with mass spectrometry (LC-MS/MS) analysis. Bongkrekic acid (BA) was an important toxin that could cause liver and kidney damages, or even death. Furthermore, there were several food poisoning incidents occurred by BA in many countries

Abstract A novel yet simple high-performance liquid chromatography (HPLC) method with diode array detector has been developed and validated for the quantification of ergosterol (Pro-vitamin D2) in commercially available lichens and cultivated edible mushrooms as per International Conference on Harmonization (ICH) guidelines. The extraction of ergosterol in the lichens and mushrooms was carried out

Abstract Sudan I and metanil yellow are azo dyes, illegally used to impart bright red and yellow color to foods respectively. It is assumed that these appearances are due to adulteration. Regulations must be able to detect the substances in a cost-effective, efficient, and sensitive manner. A simple and precise ultra-high-performance liquid chromatography with photodiode array detector based analytical

Abstract The polymer additives may migrate out from polymer plastics into foods and drinks and their potential risk should not be ignored. In this study, amino group modified magnetic mesoporous silica microspheres were prepared by an in situ method. The magnetic microspheres as adsorbent of magnetic solid-phase extraction (MSPE) were coupled with high performance liquid chromatography (HPLC) to determine

Abstract In this proof-of-concept study, new monolithic columns were prepared by incorporating fumed silica nanoparticles (FNSPs) that are surface functionalized by covalent attachment of 1-[3-(trimethoxysilyl)propyl]urea moieties (i.e., carbamide-FSNPs). The prepared monolithic columns were successfully utilized in hydrophilic interaction liquid chromatography (HILIC) of small, relatively polar solutes

Abstract Belinostat is a histone deacetylase inhibitor used for the management of hematological malignancies and solid tumors. In the current study, stability-indicating ultra-performance liquid chromatography (RP-UPLC) and developed highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) for identification, quantification and characterization of belinostat and its degradation products

Abstract In traditional Chinese therapy, the decoction of herbal products is commonly used as a therapeutic drug, which takes water as the extraction medium and contains many hydrophilic compounds. The main compounds reported in Eupatorium fortunei Turcz were volatile oils. However, polar constituents of its decoction were less studied. In this paper, polar compounds in aqueous extract of E. fortunei

Abstract A LC-MS/MS technique separated the bovine and mouse brain gangliosides monosialotetrahexosylgangliosides (GM1), disialotetrahexosylgangliosides (GD1a), trisialotetrahexosylgangliosides (GT1b) and tetrasialotetrahexosylgangliosides (GQ1b) using a phenyl-hexyl HPLC column and employing a linear methanol gradient in water, which is 0.028% in ammonium hydroxide. The gangliosides were separated

Abstract A method for quantitative determination of the main active ingredient in traditional Chinese medicine was developed and validated, using liquid-liquid chromatography combined with high performance liquid chromatography, which was successfully applied to synephrine in Fructus Aurantii Immaturus. A biphasic solvent system composed of ethyl acetate - n-butanol - water - trifluoroacetic acid (1:2:3:0

Abstract A rapid and highly sensitive modified QuEChERS method was combined with high performance liquid chromatography-ultraviolet fluorescence detection (HPLC-UV-FLD) to determine and evaluate sixteen polycyclic aromatic hydrocarbons (PAHs) in 74 traditional Chinese medicines (TCMs). A sample-based QuEChERS method pretreatment technology was used to improve sensitivity and selectivity through decolorization

Abstract The analysis method based on high performance liquid chromatography (HPLC) is an important tool for the quality control of energetic materials. 1,1'-Diamino-4,4',5,5'-tetranitro-2,2'-biimidazole (DATNBI) as a new low-sensitivity and high-energy explosive, has many potential applications in both civil and military areas. However, the method based on HPLC is not known for the analysis of DATNBI

Abstract The chromatographic analysis of a number of β-blockers on different thin-layer chromatography (TLC) systems was carried out. The analysis was done on three different systems, namely normal phase, paraffin oil impregnated in methylene chloride and n-hexane as two types of reversed phase systems. This represents an economic and environmental approach pathway for the determination of four solvents

Abstract This study aims to determine the qualitative and quantitative contents of Turkish propolis collected from various regions of Turkey using high-performance liquid chromatography with the diode-array detector (HPLC-DAD) and gas chromatography-mass spectrometry (GC-MS) in water and ethanolic extracts. In HPLC-DAD analyses, it was determined that water extract of Turkish propolis contains phenolic

Abstract A high performance liquid chromatography (HPLC) method is developed for the determination of lanthanides present in zirconium matrix. α-Hydroxyisobutyric acid (α-HIBA) is used as an eluent for the removal of bulk of zirconium and pre-concentration of lanthanides. Later the pre-concentrated lanthanides are individually separated by dynamic ion exchange method. The method offers linearity in

Abstract Simultaneous quantitative analysis of catecholamines (CAs) and their respective acid metabolites in biological fluids can become a powerful tool for diagnosis and treatment of various diseases. However, selective sample preparation procedure is necessary to isolate the aforementioned analytes from the matrix. Therefore, we propose a synthetic strategy for the preparation of a molecularly imprinted

Abstract Simultaneous quantitative analysis of catecholamines (CAs) and their respective acid metabolites in biological fluids can become a powerful tool for diagnosis and treatment of various diseases. However, selective sample preparation procedure is necessary to isolate the aforementioned analytes from the matrix. Therefore, we propose a synthetic strategy for the preparation of a molecularly imprinted

Abstract A total variation bounded (TVB) Runge–Kutta local-projection discontinuous Galerkin (RK-LDG) finite element method is applied for numerically approximating the non-isothermal and non-equilibrium model of liquid chromatography. The model comprises a system of convection-diffusion partial differential equations (PDEs) coupled with algebraic and ordinary differential equations (ODEs). The suggested

Abstract Considering the importance of robust analytics in the pharmaceutical industry, an analytical quality by design (AQbD) based reversed phase liquid chromatographic method was developed for the quantification of propranolol hydrochloride (PRO) and etizolam (ETZ). The developed method was validated and compared with an analytical method of conventional approach. In AQbD approach, analytical target

Abstract Green and efficient separation methods are gradually being loved, and microemulsion phase chromatography is used in many fields. This study used a microemulsion liquid chromatographic method for the simultaneous separation of five isoflavones (formononetin, ononin, calycosin, calycosin-7-glucoside, and biochanin A) in red clover within 10 minutes for the first time. Separation and analysis

Abstract This work is a review on HPTLC contribution to lipid analysis in complex matrices, in the period from 2010 to now, lapse of time where hyphenation with other techniques, especially Mass Spectrometry, has experienced an important growth. Scanning densitometry (SD)-UV/FL of separated lipids, as the core of detection and centerpiece for hyphenation, and its coupling with MS using soft ionization

Abstract This work is a review on HPTLC contribution to lipid analysis in complex matrices, in the period from 2010 to now, lapse of time where hyphenation with other techniques, especially Mass Spectrometry, has experienced an important growth. Scanning densitometry (SD)-UV/FL of separated lipids, as the core of detection and centerpiece for hyphenation, and its coupling with MS using soft ionization

Abstract Haloperidol is a highly potent, first-generation antipsychotic drug used for the treatment of several disorders. It exerts its antipsychotic effect through the strong antagonism in the dopamine D2 receptor. Effective analytical procedures are required for clinical and pharmaceutical studies. In this review, a variety of analytical techniques and associated methodologies for the determination

Abstract Haloperidol is a highly potent, first-generation antipsychotic drug used for the treatment of several disorders. It exerts its antipsychotic effect through the strong antagonism in the dopamine D2 receptor. Effective analytical procedures are required for clinical and pharmaceutical studies. In this review, a variety of analytical techniques and associated methodologies for the determination

Abstract This study aimed to explore phenolic acids, nutrient status, and content of photopigments and their role in the production of secondary metabolites in Selinum tenuifolium collected from different altitudes (2150 m; 2593 m; 3178 m, asl) in Chopta region of Uttarakhand, India. High-performance thin-layer chromatography (HPTLC) fingerprinting, nutrient analysis, and chlorophyll (Chl) content

Abstract Soyasapogenols derived from soyasaponins, which abundantly occur in soy hypocotyls, are of great importance as bioactive compounds due to their physiological activities. However, there is a lack of a high-efficiency and scalable preparation and isolation procedure. This work extends research on applying and comparing high-speed countercurrent chromatography (HSCCC) and preparative high-performance

Abstract One of the priority tasks of the pharmaceutical industry is to manufacturing high-quality, effective, and safe medicines. Thus, the interest in for searching biologically active compounds perspective for the creation of new drugs is growing. The aim of this work was to study the chromatographic behavior of benzimidazole and its firstly synthesized derivatives by reversed-phase–HPLC (RP-HPLC)

Abstract In this work, a polar stationary phase was prepared by thermal immobilization of poly(glycidoxypropylmethyl-co-dimethylsiloxane) onto silica particles to separate polar compounds using water-rich mobile phases. The retention properties of the stationary phase were characterized by the separation of Tanaka and NIST SRM® 870 test mixtures. The elution order of the compounds indicated the reversed-phase

Abstract Forensic ink analyst is frequently asked to determine the source of questioned ink entries. Ultra-performance liquid chromatography (UPLC) technique is useful for profiling organic compounds of pen inks. However, interpreting the UPLC chromatograms requires the use of statistical approaches in order to ensure the objectivity and reliability of the results. This work aims to compare the performances

Abstract The present work aims at developing a QbD assisted RP-HPLC method for the estimation of individual curcuminoids in Curcuma longa rhizomes along with its quality assessment. The quality of crude C. longa sample was evaluated with respect to physicochemical, phytochemical and toxic contaminant analysis. The ATP and CQA’s were identified followed by the optimization of the method by employing

The present work aims at developing a QbD assisted RP-HPLC method for the estimation of individual curcuminoids in Curcuma longa rhizomes along with its quality assessment. The quality of crude C. ...

Abstract A tryptophan ionic liquid (TrpIL) decorated magnetic graphene oxide (Fe3O4@SiO2-GO-TrpIL) nanocomposite has been prepared for magnetic solid-phase extraction of Sudan I–IV before their high performance liquid chromatography (HPLC) analysis. Fe3O4@SiO2 nanoparticles loaded on graphene oxide endow the as-prepared nanocomposite with superparamagnetic performance, benefiting conveniently magnetic

Abstract A tryptophan ionic liquid (TrpIL) decorated magnetic graphene oxide (Fe3O4@SiO2-GO-TrpIL) nanocomposite has been prepared for magnetic solid-phase extraction of Sudan I–IV before their high performance liquid chromatography (HPLC) analysis. Fe3O4@SiO2 nanoparticles loaded on graphene oxide endow the as-prepared nanocomposite with superparamagnetic performance, benefiting conveniently magnetic

Abstract In this study, lipophilicity of 21 cycloalkylspiro-5-hidantoins was assessed. The overall goal of lipophilicity evaluation is preliminary investigation which should result in a decrease of the traditionally high attrition rates for compounds entering clinical trials. Lipophilicity assessment was done by reversed-phase thin-layer chromatography and in silico methods. Chromatographic analyses

Abstract In this study, lipophilicity of 21 cycloalkylspiro-5-hidantoins was assessed. The overall goal of lipophilicity evaluation is preliminary investigation which should result in a decrease of the traditionally high attrition rates for compounds entering clinical trials. Lipophilicity assessment was done by reversed-phase thin-layer chromatography and in silico methods. Chromatographic analyses

Abstract The adsorption of mandelic acid enantiomers on the Nautilus-E chiral stationary phase with eremomycin as a grafted antibiotic, as affected by value of the water-ethanol eluent under conditions of an overloaded column, was studied. Adsorption equilibrium under experimental conditions, when mandelic acid dissociates both completely and then very weakly, was studied. The adsorption isotherms

Abstract The porous layer open-tube (PLOT) columns, which were characterized by their considerable specific surface area and high permeability, have attracted a growing attention from researchers in liquid chromatography. In this work, a hydrophilic PLOT column rich in carboxylic acid functional groups was successfully synthesized in capillary with an inner diameter of 200 µm by thermally initiated

Abstract The effect of temperature variations in liquid chromatographic columns of cylindrical geometry are studied by formulating a non-isothermal and non-equilibrium two-dimensional (2D) lumped kinetic model (LKM) simulating the flows of two-component mixtures. The developed models contain systems of coupled convection-dominated partial differential, differential, and algebraic equations for mass

Abstract In this work we proposed a novel methodological approach to detect and differentiate between cooked and uncooked animal meat protein in archaeological samples. We tested two groups of materials: ceramic dated to the early medieval period (9th–10th centuries CE) and soil samples collected from the same stratigraphic layer that yielded these pottery fragments. Using an ELISA immunological test

Abstract The selected diphenylacetamide derivatives were subjected to chromatographic and in silico approach in order to obtain significant information about their structure-activity relationships. As an early step in the assessment of their biological profile, drug-likeness and lead-likeness rules were performed, as well as determination of the bioactivity scores. The relationships between the obtained