The aim of this study was to perform qualitative and quantitative analyses of aloe-emodin, rhein, and emodin in three prepared samples of compound qi yin granules by high-performance thin-layer chromatography (HPTLC) and to establish an analytical method. TLC was used to qualitatively analyze the three major components of the compound: aloe-emodin, rhein, and emodin. HPTLC was performed to determine

In this work, a commercially available, chirally pure pharmaceutical containing carboxylic group, namely, levofloxacin was utilized as chiral selector for the enantioresolution of sotalol. The TLC plates were prepared by mixing levofloxacin in silica gel slurry. The racemic mixture of sotalol was applied on TLC plates using MeCN–MeOH–H2O (5.2:2.0:0.8, V/V; pH = 5.0) as the mobile phase at 22 (± 2) °C

Nowadays, the implementation of quality by design approach in analytical method development based on principles of quality risk management (QRM) and design of experiments (DoE) has became a regulatory requirement. Hence, a precise, accurate and robust high-performance thin-layer chromatography method has been developed for the simultaneous estimation of azilsartan medoxomil (AZM) and chlorthalidone

The present study was carried out for screening an efficient extraction method and extracting solvent in different plant parts of Justicia adhatoda L. for the development of a rapid and simultaneous determination of vasicine and vasicinone using a validated high-performance thin-layer chromatography (HPTLC) method. Four different extraction techniques, viz., maceration extraction (ME), ultrasonic-assisted

The genus Justicia (Acanthaceae) is a source of important pharmaceutical compounds. Justicia species are integral parts of several drug preparations in Indian indigenous healthcare system. However, J. beddomei and J. adhatoda are the authentic sources of ‘Vasa’. To authenticate assured therapeutic effect, proper identification at species level is critical. Phytochemical studies were carried out in

Tris(2-chloroethyl)amine is a chemical warfare agent which is considered to be a persistent contaminant highly resistant to decontamination. The time dependence of tris(2-chloroethyl)amine degradation on the water pH value is observed to determine decontamination options using the decontamination of hydroxide-based mixtures which are used by a number of armed forces. The kinetics was observed using

Two validated, simple and precise densitometric high-performance thin-layer chromatography (HPTLC) quantification methods were proposed for both qualitative and quantitative estimation of oleuropein in Olea europaea leaves and a pharmaceutical product utilizing normal-phase and reversed-phase silica gel TLC plates. In method I, 10 × 20 cm glass plates coated with 0.2 mm thin layers of normal-phase

In the present work, a simple, rapid, reliable and accessible high-performance thin-layer chromatography (HPTLC) technique has been established for the concurrent quantification of plumbagin. The well-known medicinal plant, Plumbago zeylanica L., has been traditionally used to treat a variety of ailments in India and other countries. Chromatographic separation was conceded on pre-coated silica gel

A high-performance thin-layer chromatography method for the simultaneous quantitative determination and validation of scopoletin and gallic acid in the methanolic fraction of Jatropha glandulifera L. was developed for the first time. For achieving good separation, the mobile phase of toluene‒ethyl acetate‒glacial acetic acid (7.5:2.5:0.1, V/V) was used. Densitometric determination was carried out at

A novel, rapid, and sensitive stability-indicating high-performance thin-layer chromatography method was developed and validated for the simultaneous determination of celecoxib (CEL) and diacerein (DIA) in bulk and a capsule binary mixture. The complete separation was achieved by the proposed method on nanosilica gel F254 plates using n-hexane‒ethyl acetate‒tetrahydrofuran‒glacial acetic acid (7:3:1:0

Many specific and non-specific chromogenic spray reagents have been used to detect organic pesticides (organophosphorus, organochlorine, carbamates, and pyrethroids) on thin-layer chromatographic (TLC)/high-performance thin-layer chromatographic (HPTLC) plates. To realize high sensitivity and improved selectivity, several chromogenic reagents have been introduced. The physical properties of organic

Medicinal plants of the Cucurbitaceae family are widely consumed as foods in human diet. In the present study, a simple, rapid, quantitative, and validated high-performance thin-layer chromatographic (HPTLC) method has been developed for the estimation of chlorogenic acid (CA) in the hydroalcoholic fruit extracts of Luffa acutangula (sponge gourd), Sechium edule (chayote squash), Trichosanthes cucumerina

The present study focuses on the development and validation of a high-performance thin-layer chromatography (HPTLC)‒densitometry method for the identification and quantification of resveratrol in the 70% methanolic extract of Morus alba fruits. The separation was achieved with the developer hexane‒ethyl acetate‒glacial acetic acid (4:6:0.1, V/V) on TLC aluminum plate pre-coated with silica gel 60 F254

The rapid and handy high-performance thin-layer chromatography (HPTLC) method has been widely adopted to qualitatively identify herbs or medicinal materials from Chinese patent medicine. Weikangling capsules, as one of the traditional Chinese patent medicines for treating chronic gastritis, consist of Paeoniae Radix Alba, Glycyrrhizae Radix et Rhizoma, Bletillae Rhizoma, Notoginseng Radix et Rhizoma

The species Sclerocarya birrea, also known as Marula, is present in Sub-Saharan Africa. In Burkina Faso, the leaves are sold as a traditional improved medicine with antidiabetic indication. The objective of this study was to develop a simple, rapid and robust high-performance thin-layer chromatographic (HPTLC) method for the reliable quality control of S. birrea leaves in Burkina Faso and other West

Green analytical methods have gained a growing interest in the field of pharmaceutical research to reduce impacts on the environment and enhance analysts’ health safety. Chloramphenicol (CHL), dexamethasone sodium phosphate (DSP) and tetrahydrozoline HCl (THZ) form an ophthalmic ternary mixture that is co-formulated for conjunctivitis treatment. In the present work, for time saving and higher sensitivity

This review describes the application of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) in the separation and identification of vitamins from their mixtures using different kinds of mobile phases such as isocratic and mixed solvents as well as mobile phases containing various modifiers. TLC has long been used for the separation and semiquantitative determination

Sugars, in particular glucose and fructose, are the main constituents of honey, comprising up to 85% of its total weight. A high-performance thin-layer chromatography (HPTLC) method to identify and quantify common sugars (glucose, fructose and sucrose) in honey has been developed and fully validated according to the International Conference on Harmonisation guidelines. It allows to determine a honey’s

Misuse and abuse of prescription drugs has become a global health problem in the last few years as the illicit drug cartels have grown exponentially. The non-medical use of prescribed medications, such as benzodiazepines, synthetic opioids, stimulants and tranquilizers, has increased rapidly due to their easy availability. Nowadays benzodiazepines are not only misused/abused, but also play a significant

This review article describes the work reported on the separation and analysis of heavy metal ions by different modes of thin-layer chromatography (TLC) such as soil, micellar, and reversed-phase TLC during 2000–2019. The history of different modes of TLC has been briefly mentioned. The literature survey reveals that the number of papers reported during 2000–2019 on the separation of heavy metal ions

Drug abuse is a global menace in the society. Strict measures are planned and effectuated to dismantle this crime yet a large number of cases of narcotics drugs are seized and referred to forensic science laboratories for analysis. Over the past few years, the misuse of precursor chemicals has increased substantially. Examination of these precursor chemicals, especially ephedrine and its analogues

High-throughput and sensitive determination of triterpenoids is challenging by high-performance liquid chromatographic methods due to their non-chromophoric nature, lipophilic properties, and sequential run. The present study demonstrates the development of a validated high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination of bioactive terpenoids in the bark of

A highly sensitive, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the quantitative determination of adapalene in the presence of its degradation products. The method employed precoated silica gel G 60 F254 on aluminum sheets using an appropriate solvent system which gives a compact spot of adapalene at RF value 0.58. The densitometric

Food adulteration is a primary global concern for public health, especially in developing countries, due to the lack of monitoring and appropriate policy developments and implementations. Synthetic colors and dyes are used to enhance the appearance of food products. Metanil Yellow (MY), a non-permitted, toxic, chief additive adulterant is majorly used in sweets, pulses, and turmeric in India owing

The present research paper includes the development, validation, and comparison of three analytical methods including spectrofluorimetric, ultraviolet (UV)-spectrophotometric, and spectrodensitometric methods based on high-performance thin-layer chromatographic (HPTLC) technique for the estimation of riociguat in tablet dosage form. The estimation was performed at wavelength maxima of 322 nm for UV-spectrophotometry

The thin-layer chromatographic (TLC) analysis of extracts/compounds has several applications in the field of food, cosmetics, phytochemistry, and pharmaceutical industry. Mangiferin is a bioactive and natural antioxidant polyphenolic xanthone obtained from different dietary plants and recently gaining remarkable attention due to its high pharmacological significance evident in current scientific validations

Duloxetine hydrochloride is an anti-depressant drug, while 1-naphthol is one of its hepatotoxic impurities. So, there was an urgent need to do simultaneous analysis for both the drug and its toxic impurity. Therefore, a validated high-performance thin-layer chromatography (HPTLC) method was established for the simultaneous separation and quantification of duloxetine hydrochloride and 1-naphthol in

Crataeva nurvala Buch-Ham. (Capparidaceae) is an important medicinal plant, the bark of which is extensively used in Ayurveda as a remedy for kidney stones, urinary tract infections, prostate-related disorders, and benign prostatic hypertrophy. In this study, a sensitive and reliable high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous quantification of

Azo dyes, in spite of being additives, are indeed an integral constituent of consumer goods with bio-therapeutic values. The addition of dyes is for consumer compliance which lends a sense of pleasing aesthetics to the consumption ready product, “a kind of placebo effect”. Consequently, imperative need of the hour is felt to quantify such azo dyes due to complications like skin allergic reactions,

A novel flow chromatographic immunoassay method using end-point dilution was developed in our laboratory as an alternative to the homogeneous enzyme immunoassay, for low-cost, simple, and fast semi-quantitative analyses of drugs of abuse in human urine. A serial dilution of the positive test sample using rapid test cassettes is continued until reaching the lowest dilution, with a visual detectable

Radix Astragali is a traditional Chinese medicine (TCM), which strengthens the efficacy in tonifying Qi after honey-processing. However, a comparative study on the marker compounds in raw and honey-processed Radix Astragali has not been reported. A rapid and sensitive high-performance thin-layer chromatographic (HPTLC) method has been established for the analysis of astragaloside IV and calycosin-7-O-β-glucoside

Herbal medicine is gaining its popularity day by day and gradually streaming toward integration into the mainstream healthcare systems. Therefore, standardization of herbal formulations is essential in order to assess quality of the drugs. Two commonly used paradigms, compound and pattern-based approaches, were performed to ensure quality and hence product consistency. A rapid, sensitive, and reproducible

Quality risk assessment and design of experiment (DoE)-based quality by design (QbD) approach has been applied for the development of a stability-indicating assay method for acidic degradation kinetic study of apremilast. Quality risk assessment started with the identification of risk factors for method development by preliminary experimentation, prior knowledge of chromatography, and brainstorming

The aim of this study was the separation of cholic, ursodeoxycholic, chenodeoxycholic, deoxycholic, and lithocholic bile acids by thin-layer chromatography (TLC) with mobile phases based on surfactants. The effect of surfactant type and concentration, the pH of the mobile phase, the type and amount of the mobile phase modifier, and the effect of temperature and type of material backed silica gel TLC

The present study was designed to develop and validate a high-performance thin-layer chromatography (HPTLC) system for the simultaneous quantitative determination of berberine and rutin in Tinospora cordifolia extract and their pharmaceutical preparations. Chromatographic development was done using a blend of n-hexane, ethyl acetate, glacial acetic acid and methanol (10:1.1:1.1:2.5, v/v) as the mobile

A sensitive and reliable high-performance thin-layer chromatography method has been developed for the simultaneous estimation of bavachin, bakuchiol, and psoralen in Psoralea corylifolia seeds. The methanolic extract of the seeds was applied on a silica gel G 60 F254 plate. The plate was developed using toluene–ether, 1:1, saturated with 10% acetic acid (v/v) as the mobile phase and detection and quantification

Using honey as a model sample, the aim of this study is to develop and validate a simple, rapid screening tool that allows for the visualization of constituents that contribute to the antioxidant activity of a complex natural product and to quantify their individual antioxidant effects even if their chemical identity is unknown. The method employs a validated analysis, which is based on the separation

It is known that textile dyes have various risks on human health. Glutathione-S-transferase enzymes play a critical role in the detoxification of xenobiotics in living systems. This study aimed to examine the interaction of methylene blue with human erythrocyte glutathione-S-transferase purified in one step. Human erythrocyte glutathione-S-transferase was purified with approximately 750-fold purification

No published studies reported triazophos (TZP) analysis in human whole blood following fatal intoxication. In this study, a method for the determination of TZP in human whole blood using liquid–liquid extraction (LLE) and thin-layer chromatography (TLC)–ultraviolet (UV) densitometry is described. Chromatography was performed on silica gel 60F254 TLC plates using mobile phase n-hexane:acetone (8:2,

Mycotoxin contamination is an ongoing problem for all kinds of food commodity, and it is necessary to investigate the extent of this problem in baby food products. It is seen that factors such as developing technology, urbanization, and intense work tempo change dietary habits in every age group. The aim of this study was to provide a current overview on patulin (PAT) contamination of fruit-based baby

Paying attention to issue of environmental protection is an unavoidable duty which encourages analytical society to balance between establishment of new analytical method and preservation of the environment from the harmful chemicals. By adopting this concept, an excellent green high-performance thin-layer chromatography (HPTLC) method was developed for assessment of carbamazepine, the antiepileptic

Retention behaviour of twenty-one chalcones synthesized in our laboratory was tested in three thin-layer chromatography (RP-TLC) systems (acetonitrile–water, ethanol–water and acetone–water) and chromatography parameters \( {R}_M^0 \), S and C0 were calculated. The most suitable RP-TLC system (acetonitrile–water) and chromatography parameter (C0) for lipophilicity prediction of tested compounds were

Green validated high-performance thin-layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) methods were established for resolving ephedrine hydrochloride (EPH), naphazoline nitrate (NAP), and methylparaben (MET) mixture. Method A is HPTLC–densitometric one that depends on their separation and quantitation on HPTLC silica gel 60 F254 plates, using ethyl acetate:ethanol:triethylamine

A sensitive, accurate, precise, and robust high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous quantification of empagliflozin, linagliptin, and metformin HCl in bulk and synthetic mixture. The method applied HPTLC aluminum plates pre-coated with silica gel 60 F254 as the stationary phase along with n-butanol:water:glacial acetic acid (6:3:1, v/v) as the

A stability-indicating high-performance thin-layer chromatography (HPTLC) method was developed and validated for determination of arterolane maleate and piperaquine phosphate in combined dosage form. This study was carried out by using the mobile phase containing isopropyl alcohol:n-butanol:methanol:triethylamine (3:6:1:0.2 v/v). The method was validated as per the International Conference on Harmonization

The purpose of the present study was to develop a simple, rapid and sensitive high-performance thin-layer chromatography (HPTLC) method for the simultaneous estimation of 5-fluorouracil (5FU) and berberine (BER) in rabbit plasma. BER and 5-FU are currently under extensive studies for their synergistic effects in cancer chemotherapy. The HPTLC method was developed using TLC aluminum plates precoated

Stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) and normal-phase high-performance thin-layer chromatography (NP-HPTLC) methods have been developed for the determination of palonosetron which is a potent antiemetic drug used with chemotherapy. Forced degradation studies were performed on palonosetron to study its stability behavior. The drug was subjected to acid

The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary

This paper deals with chromatographic method development for the simultaneous estimation of levocetrizine hydrochloride and montelukast sodium in their combined dosage form using analytical quality by design approach. The main objective for application of analytical quality by design approach was to identify conditions where an adequate separation could be achieved within a robust region. Critical

The metabolite contents of the plants may vary with season and geographic variations, and this induces the biological potential of the plant species. The present study aimed to identify the elite chemotype(s) of Plumbago zeylanica, collected from the natural habitat of Gangetic plains on the basis of plumbagin content. The effect of intra-specific variability in the plumbagin content among the population

The synthetic pesticide profenofos is a member of organophosphate insecticide compounds. It is an important synthetic insecticide and has a diversified role in agriculture. During the last decade, profenofos was frequently misused in homicidal, accidental, and suicidal poisoning cases. Since then, a large number of visceral samples have been received for toxicological chemical analysis. Thin-layer

Enantiomeric separation of (RS)-atenolol, and (RS)-propranolol has been achieved by thin-layer chromatography (TLC) using both direct and indirect modes. (S)-naproxen, containing a carboxylic group, was utilized as the chiral selector. For direct TLC separation, (S)-naproxen was used as a chiral additive in the stationary phase in a non-covalent mode and there was no chiral additive in the mobile phase;

Superfine pulverization and ordinary pulverization were used to obtain powders with different particle sizes in different parts of Fructus rosae laxae. High-performance thin-layer chromatography (HPTLC) was used to carry out a simple, fast, qualitative, and quantitative analysis of vitamin C in Fructus rosae laxae. The effects of superfine pulverization and ordinary pulverization on the vitamin C content

Accurate, simple, and selective spectrophotometric and spectrodensitometric methods were developed and adopted to quantify velpatasvir (VPS) and sofosbuvir (SFV) concurrently in their pure forms and tablets. The spectrophotometric technique was based on the first derivative of ratio spectra (1DD) technique and developed to determine VPS and SFV simultaneously in table formulation. However, the spectrodensitometric

A new, simple, precise, accurate and selective high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for the simultaneous determination of ledipasvir and sofosbuvir in their tablet dosage form. Chromatographic separation was carried out on Merck TLC aluminum sheets of silica gel 60 F254 using ethyl acetate:hexane:methanol in the ratio of 8:1.25:0.75 (% v/v) as

HPTLC is a useful and practical analytical tool to characterize plant compositions. This study was focused on exploring the results of high-performance thin-layer chromatography (HPTLC) analysis, particularly as a useful tool for the authentication of Allanblackia parviflora seed and kernel cakes. Bulked samples from sixteen different Ghanaian communities were analysed by HPTLC and their fingerprints

Ethyl p-methoxycinnamate (EPMC) is the main active ingredient in Kaempferia galanga rhizome (KGR). At present, the correlation study of the component by high-performance liquid chromatography (HPLC) has been reported. High-performance thin-layer chromatographic scanning (HPTLCS) method has been widely used for the qualitative and quantitative analysis of many chemical components, but has not yet been

An HPTLC (high-performance thin-layer chromatography) method was carried out to develop a feasible, simple and accurate method for the estimation of exact concentration of flavonoids in Moringa oleifera. The simultaneous determination of rutin, chlorogenic acid, and gallic acid extracted from different parts (leaf, stem, flower, and root) of M. oleifera was performed and analysis was carried out on

Thin-layer chromatography (TLC) hyphenated to bioassays is a modern tool used for discovery of drugs with diverse biological activities from plant extracts which justifies the continuous need for developing such methods. A new TLC–bioautography assay for detecting peroxidase enzyme inhibitors was developed and validated in this study. Peroxidases are related to pathogenesis of many diseases such as