Crataeva nurvala Buch-Ham. (Capparidaceae) is an important medicinal plant, the bark of which is extensively used in Ayurveda as a remedy for kidney stones, urinary tract infections, prostate-related disorders, and benign prostatic hypertrophy. In this study, a sensitive and reliable high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous quantification of

Azo dyes, in spite of being additives, are indeed an integral constituent of consumer goods with bio-therapeutic values. The addition of dyes is for consumer compliance which lends a sense of pleasing aesthetics to the consumption ready product, “a kind of placebo effect”. Consequently, imperative need of the hour is felt to quantify such azo dyes due to complications like skin allergic reactions,

A novel flow chromatographic immunoassay method using end-point dilution was developed in our laboratory as an alternative to the homogeneous enzyme immunoassay, for low-cost, simple, and fast semi-quantitative analyses of drugs of abuse in human urine. A serial dilution of the positive test sample using rapid test cassettes is continued until reaching the lowest dilution, with a visual detectable

Radix Astragali is a traditional Chinese medicine (TCM), which strengthens the efficacy in tonifying Qi after honey-processing. However, a comparative study on the marker compounds in raw and honey-processed Radix Astragali has not been reported. A rapid and sensitive high-performance thin-layer chromatographic (HPTLC) method has been established for the analysis of astragaloside IV and calycosin-7-O-β-glucoside

Herbal medicine is gaining its popularity day by day and gradually streaming toward integration into the mainstream healthcare systems. Therefore, standardization of herbal formulations is essential in order to assess quality of the drugs. Two commonly used paradigms, compound and pattern-based approaches, were performed to ensure quality and hence product consistency. A rapid, sensitive, and reproducible

Quality risk assessment and design of experiment (DoE)-based quality by design (QbD) approach has been applied for the development of a stability-indicating assay method for acidic degradation kinetic study of apremilast. Quality risk assessment started with the identification of risk factors for method development by preliminary experimentation, prior knowledge of chromatography, and brainstorming

The aim of this study was the separation of cholic, ursodeoxycholic, chenodeoxycholic, deoxycholic, and lithocholic bile acids by thin-layer chromatography (TLC) with mobile phases based on surfactants. The effect of surfactant type and concentration, the pH of the mobile phase, the type and amount of the mobile phase modifier, and the effect of temperature and type of material backed silica gel TLC

The present study was designed to develop and validate a high-performance thin-layer chromatography (HPTLC) system for the simultaneous quantitative determination of berberine and rutin in Tinospora cordifolia extract and their pharmaceutical preparations. Chromatographic development was done using a blend of n-hexane, ethyl acetate, glacial acetic acid and methanol (10:1.1:1.1:2.5, v/v) as the mobile

A sensitive and reliable high–performance thin–layer chromatography method has been developed for the simultaneous estimation of bavachin, bakuchiol, and psoralen in Psoralea corylifolia seeds. The methanolic extract of the seeds was applied on a silica gel G 60 F254 plate. The plate was developed using toluene–ether, 1:1, saturated with 10% acetic acid (v/v) as the mobile phase and detection and quantification

Using honey as a model sample, the aim of this study is to develop and validate a simple, rapid screening tool that allows for the visualization of constituents that contribute to the antioxidant activity of a complex natural product and to quantify their individual antioxidant effects even if their chemical identity is unknown. The method employs a validated analysis, which is based on the separation

It is known that textile dyes have various risks on human health. Glutathione-S-transferase enzymes play a critical role in the detoxification of xenobiotics in living systems. This study aimed to examine the interaction of methylene blue with human erythrocyte glutathione-S-transferase purified in one step. Human erythrocyte glutathione-S-transferase was purified with approximately 750-fold purification

No published studies reported triazophos (TZP) analysis in human whole blood following fatal intoxication. In this study, a method for the determination of TZP in human whole blood using liquid–liquid extraction (LLE) and thin-layer chromatography (TLC)–ultraviolet (UV) densitometry is described. Chromatography was performed on silica gel 60F254 TLC plates using mobile phase n-hexane:acetone (8:2,

Mycotoxin contamination is an ongoing problem for all kinds of food commodity, and it is necessary to investigate the extent of this problem in baby food products. It is seen that factors such as developing technology, urbanization, and intense work tempo change dietary habits in every age group. The aim of this study was to provide a current overview on patulin (PAT) contamination of fruit-based baby

Paying attention to issue of environmental protection is an unavoidable duty which encourages analytical society to balance between establishment of new analytical method and preservation of the environment from the harmful chemicals. By adopting this concept, an excellent green high-performance thin-layer chromatography (HPTLC) method was developed for assessment of carbamazepine, the antiepileptic

Retention behaviour of twenty-one chalcones synthesized in our laboratory was tested in three thin-layer chromatography (RP-TLC) systems (acetonitrile–water, ethanol–water and acetone–water) and chromatography parameters \( {R}_M^0 \), S and C0 were calculated. The most suitable RP-TLC system (acetonitrile–water) and chromatography parameter (C0) for lipophilicity prediction of tested compounds were

A sensitive, accurate, precise, and robust high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous quantification of empagliflozin, linagliptin, and metformin HCl in bulk and synthetic mixture. The method applied HPTLC aluminum plates pre-coated with silica gel 60 F254 as the stationary phase along with n-butanol:water:glacial acetic acid (6:3:1, v/v) as the

A stability-indicating high-performance thin-layer chromatography (HPTLC) method was developed and validated for determination of arterolane maleate and piperaquine phosphate in combined dosage form. This study was carried out by using the mobile phase containing isopropyl alcohol:n-butanol:methanol:triethylamine (3:6:1:0.2 v/v). The method was validated as per the International Conference on Harmonization

The purpose of the present study was to develop a simple, rapid and sensitive high-performance thin-layer chromatography (HPTLC) method for the simultaneous estimation of 5-fluorouracil (5FU) and berberine (BER) in rabbit plasma. BER and 5-FU are currently under extensive studies for their synergistic effects in cancer chemotherapy. The HPTLC method was developed using TLC aluminum plates precoated

Stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) and normal-phase high-performance thin-layer chromatography (NP-HPTLC) methods have been developed for the determination of palonosetron which is a potent antiemetic drug used with chemotherapy. Forced degradation studies were performed on palonosetron to study its stability behavior. The drug was subjected to acid

The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary

This paper deals with chromatographic method development for the simultaneous estimation of levocetrizine hydrochloride and montelukast sodium in their combined dosage form using analytical quality by design approach. The main objective for application of analytical quality by design approach was to identify conditions where an adequate separation could be achieved within a robust region. Critical

The metabolite contents of the plants may vary with season and geographic variations, and this induces the biological potential of the plant species. The present study aimed to identify the elite chemotype(s) of Plumbago zeylanica, collected from the natural habitat of Gangetic plains on the basis of plumbagin content. The effect of intra-specific variability in the plumbagin content among the population

The synthetic pesticide profenofos is a member of organophosphate insecticide compounds. It is an important synthetic insecticide and has a diversified role in agriculture. During the last decade, profenofos was frequently misused in homicidal, accidental, and suicidal poisoning cases. Since then, a large number of visceral samples have been received for toxicological chemical analysis. Thin-layer

Enantiomeric separation of (RS)-atenolol, and (RS)-propranolol has been achieved by thin-layer chromatography (TLC) using both direct and indirect modes. (S)-naproxen, containing a carboxylic group, was utilized as the chiral selector. For direct TLC separation, (S)-naproxen was used as a chiral additive in the stationary phase in a non-covalent mode and there was no chiral additive in the mobile phase;

Superfine pulverization and ordinary pulverization were used to obtain powders with different particle sizes in different parts of Fructus rosae laxae. High-performance thin-layer chromatography (HPTLC) was used to carry out a simple, fast, qualitative, and quantitative analysis of vitamin C in Fructus rosae laxae. The effects of superfine pulverization and ordinary pulverization on the vitamin C content