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  • The Use of Fluorescently Labeled ARC1779 Aptamer for Assessing the Effect of H 2 O 2 on von Willebrand Factor Exocytosis
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-31
    Piotr P. Avdonin, Sergey K. Trufanov, Elena Yu. Rybakova, Aleksandra A. Tsitrina, Nikolay V. Goncharov, Pavel V. Avdonin

    Abstract Here, we propose a new approach for quantitative estimation of von Willebrand factor (vWF) exposed on the surface of endothelial cells (ECs) using the ARC1779 aptamer that interacts with the vWF A1 domain. To visualize complex formation between vWF and the aptamer, the latter was conjugated with the Cy5 fluorescent label. Cultured human umbilical vein endothelial cells (HUVEC) were stained

    更新日期:2020-12-31
  • Free SARS-CoV-2 Spike Protein S1 Particles May Play a Role in the Pathogenesis of COVID-19 Infection
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-30
    Andrey V. Letarov, Vladislav V. Babenko, Eugene E. Kulikov

    Abstract The imbalance of the renin-angiotensin system is currently considered as a potentially important factor of the pathogenesis of COVID-19 disease. It has been shown previously in the murine model, that the expression of angiotensin-converting enzyme 2 (ACE2) on the cell surface is downregulated in response to the infection by SARS-CoV virus or recombinant spike protein (S protein) alone. In

    更新日期:2020-12-30
  • Structure and Expression of Large (+)RNA Genomes of Viruses of Higher Eukaryotes
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-30
    Alexey A. Agranovsky

    Abstract Viral positive-sense RNA genomes evolve rapidly due to the high mutation rates during replication and RNA recombination, which allowing the viruses to acquire and modify genes for their adaptation. The size of RNA genome is limited by several factors, including low fidelity of RNA polymerases and packaging constraints. However, the 12-kb size limit is exceeded in the two groups of eukaryotic

    更新日期:2020-12-30
  • Identification of Na + -Pumping Cytochrome Oxidase in the Membranes of Extremely Alkaliphilic Thioalkalivibrio Bacteria
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    M. S. Muntyan, D. A. Morozov, Y. F. Leonova, T. V. Ovchinnikova

    Abstract For the first time, the functioning of the oxygen reductase Na+-pump (Na+-pumping cytochrome c oxidase of the cbb3-type) was demonstrated by examining the respiratory chain of the extremely alkaliphilic bacterium Thioalkalivibrio versutus [Muntyan, M. S., et al. (2015) Cytochrome cbb3 of Thioalkalivibrio is a Na+-pumping cytochrome oxidase, Proc. Natl. Acad. Sci. USA, 112, 7695-7700], a product

    更新日期:2020-12-28
  • Mitoptosis, Twenty Years After
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    K. G. Lyamzaev, D. A. Knorre, B. V. Chernyak

    Abstract In 1999 V. P. Skulachev proposed the term “mitoptosis” to refer to the programmed elimination of mitochondria in living cells. According to the initial thought, mitoptosis serves to protect cells from malfunctioning of the damaged mitochondria. At the same time, a new mechanism of the complete mitochondria elimination was found under the conditions of massive mitochondrial damage associated

    更新日期:2020-12-28
  • Autophagy as a Target for the Retinoprotective Effects of the Mitochondria-Targeted Antioxidant SkQ1
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    D. V. Telegina, O. S. Kozhevnikova, A. Zh. Fursova, N. G. Kolosova

    Abstract Age-related macular degeneration (AMD) is a complex neurodegenerative disease, a main cause of vision loss in elderly people. The pathogenesis of dry AMD, the most common form of AMD (~ 80% cases), involves degenerative changes in the retinal pigment epithelium (RPE), which are closely associated with the age-associated impairments in autophagy. Reversion of these degenerative changes is considered

    更新日期:2020-12-28
  • What Can We Learn about Aging and COVID-19 by Studying Mortality?
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    L. A. Gavrilov, N. S. Gavrilova

    Abstract Promising ideas and directions for further research into biology of aging are discussed using analysis of the age-related kinetics of organisms’ mortality. It is shown that the traditional evolutionary theory explaining aging by decreasing force of natural selection with age is not consistent with the data on age-related mortality kinetics. The hypothesis of multistage destruction of organisms

    更新日期:2020-12-28
  • Targeting Inflammation and Oxidative Stress as a Therapy for Ischemic Kidney Injury
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    N. V. Andrianova, D. B. Zorov, E. Y. Plotnikov

    Abstract Inflammation and oxidative stress are the main pathological processes that accompany ischemic injury of kidneys and other organs. Based on this, these factors are often chosen as a target for treatment of acute kidney injury (AKI) in a variety of experimental and clinical studies. Note, that since these two components are closely interrelated during AKI development, substances that treat one

    更新日期:2020-12-28
  • Do Multiple Drug Resistance Transporters Interfere with Cell Functioning under Normal Conditions?
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    D. A. Knorre, K. V. Galkina, T. Shirokovskikh, A. Banerjee, R. Prasad

    Abstract Eukaryotic cells rely on multiple mechanisms to protect themselves from exogenous toxic compounds. For instance, cells can limit penetration of toxic molecules through the plasma membrane or sequester them within the specialized compartments. Plasma membrane transporters with broad substrate specificity confer multiple drug resistance (MDR) to cells. These transporters efflux toxic compounds

    更新日期:2020-12-28
  • Importance and Meaning of TERRA Sequences for Aging Mechanisms
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    G. Libertini, G. Corbi, F. Nicola

    Abstract Any theory suggesting an adaptive meaning for aging implicitly postulates the existence of specific mechanisms, genetically determined and modulated, causing progressive decline of an organism. According to the subtelomere–telomere theory, each telomere is covered by a hood formed in the first cell of an organism having a size preserved at each subsequent duplication. Telomere shortening,

    更新日期:2020-12-28
  • Metabolic ROS Signaling: To Immunity and Beyond
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    A. Y. Andreyev, Y. E. Kushnareva, N. N. Starkova, A. A. Starkov

    Abstract Metabolism is a critical determinant of immune cell functionality. Immunometabolism, by definition, is a multidisciplinary area of immunology research that integrates the knowledge of energy transduction mechanisms and biochemical pathways. An important concept in the field is metabolic switch, a transition of immune cells upon activation to preferential utilization of select catabolic pathways

    更新日期:2020-12-28
  • Effect of Membrane Environment on the Ligand-Binding Properties of the Terminal Oxidase Cytochrome bd -I from Escherichia coli
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    V. B. Borisov

    Abstract Cytochrome bd-I is a terminal oxidase of the Escherichia coli respiratory chain. This integral membrane protein contains three redox-active prosthetic groups (hemes b558, b595, and d) and couples the electron transfer from quinol to molecular oxygen to the generation of proton motive force, as one of its important physiological functions. The study was aimed at examining the effect of the

    更新日期:2020-12-28
  • Linking 7-Nitrobenzo-2-oxa-1,3-diazole (NBD) to Triphenylphosphonium Yields Mitochondria-Targeted Protonophore and Antibacterial Agent
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    I. R. Iaubasarova, L. S. Khailova, P. A. Nazarov, T. I. Rokitskaya, D. N. Silachev, T. I. Danilina, E. Y. Plotnikov, S. S. Denisov, R. S. Kirsanov, G. A. Korshunova, E. A. Kotova, D. B. Zorov, Y. N. Antonenko

    Abstract Appending lipophilic cations to small molecules has been widely used to produce mitochondria-targeted compounds with specific activities. In this work, we obtained a series of derivatives of the well-known fluorescent dye 7-nitrobenzo-2-oxa-1,3-diazole (NBD). According to the previous data [Denisov et al. (2014) Bioelectrochemistry, 98, 30-38], alkyl derivatives of NBD can uncouple isolated

    更新日期:2020-12-28
  • Nonphosphorylating Oxidation in Mitochondria and Related Processes
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    D. B. Zorov, N. V. Andrianova, V. A. Babenko, L. E. Bakeeva, S. D. Zorov, L. D. Zorova, I. B. Pevsner, V. A. Popkov, E. Yu. Plotnikov, D. N. Silachev

    Abstract The mechanism of oxidative phosphorylation and its regulation remain one of the main problems of bioenergetics. Efficiency of the mitochondrial energization is determined by the relationship between the rate of generation of electrochemical potential of hydrogen ions and the rate of its expenditure on the synthesis of ATP and the use of ATP in endergonic reactions. Uncoupling (partial or complete)

    更新日期:2020-12-28
  • Expansion of the “Sodium World” through Evolutionary Time and Taxonomic Space
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    M. I. Kozlova, I. M. Bushmakin, J. D. Belyaeva, D. N. Shalaeva, D. V. Dibrova, D. A. Cherepanov, A. Y. Mulkidjanian

    Abstract In 1986, Vladimir Skulachev and his colleagues coined the term “Sodium World” for the group of diverse organisms with sodium (Na)-based bioenergetics. Albeit only few such organisms had been discovered by that time, the authors insightfully noted that “the great taxonomic variety of organisms employing the Na-cycle points to the ubiquitous distribution of this novel type of membrane-linked

    更新日期:2020-12-28
  • At the Crossroads of Bioenergetics and Antibiotic Discovery
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    K. Lewis

    Abstract Dr. Vladimir Skulachev was my mentor, and his pioneering work in the field of bioenergetics inspired the discoveries described in this review, written in the form of a personal account of events. Examining basic mechanisms of chemiosmotic coupling unexpectedly led us to transenvelope multidrug resistance pumps (MDR pumps) that severely limit development of novel antibiotics. One of the major

    更新日期:2020-12-28
  • Nobiletin: Targeting the Circadian Network to Promote Bioenergetics and Healthy Aging
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    E. Mileykovskaya, S.-H. Yoo, W. Dowhan, Z. Chen

    Abstract The circadian clock is the biological mastermind governing orderly execution of bodily processes throughout the day. In recent years, an emerging topic of broad interest is clock-modulatory agents, including small molecules both of synthetic and natural origins, and their potential applications in disease models. Nobiletin is a naturally occurring flavonoid with the greatest abundance found

    更新日期:2020-12-28
  • Rotary Ion-Translocating ATPases/ATP Synthases: Diversity, Similarities, and Differences
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    V. M. Zubareva, A. S. Lapashina, T. E. Shugaeva, A. V. Litvin, B. A. Feniouk

    Abstract Ion-translocating ATPases and ATP synthases (F-, V-, A-type ATPases, and several P-type ATPases and ABC-transporters) catalyze ATP hydrolysis or ATP synthesis coupled with the ion transport across the membrane. F-, V-, and A-ATPases are protein nanomachines that combine transmembrane transport of protons or sodium ions with ATP synthesis/hydrolysis by means of a rotary mechanism. These enzymes

    更新日期:2020-12-28
  • COVID-19 and Oxidative Stress
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-12-28
    B. V. Chernyak, E. N. Popova, A. S. Prikhodko, O. A. Grebenchikov, L. A. Zinovkina, R. A. Zinovkin

    Abstract Pathogenesis of the novel coronavirus infection COVID-19 is the subject of active research around the world. COVID-19 caused by the SARS-CoV-2 is a complex disease in which interaction of the virus with target cells, action of the immune system and the body’s systemic response to these events are closely intertwined. Many respiratory viral infections, including COVID-19, cause death of the

    更新日期:2020-12-28
  • Overcoming Antibiotic Resistance in Microorganisms: Molecular Mechanisms
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    A. G. Gabibov, O. A. Dontsova, A. M. Egorov

    Abstract This issue of the Biochemistry (Moscow) journal presents reviews and experimental articles on the new strategies for solving the problem of antibiotic resistance and on the search for novel antimicrobial preparations using the methods of molecular biology, genetics, and nanotechnology. A wide variety of scientific approaches and successful (as a rule) research results give hope for overcoming

    更新日期:2020-11-27
  • Bacteriophages as Therapeutic Preparations: What Restricts Their Application in Medicine
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    V. V. Vlassov, N. V. Tikunova, V. V. Morozova

    Abstract The increasing prevalence of bacterial pathogens with multiple antibiotic resistance requires development of new approaches to control infections. Phage therapy is one of the most promising approaches. In recent years, research organizations and a number of pharmaceutical companies have intensified investigations aimed at developing bacteriophage-based therapeutics. In the United States and

    更新日期:2020-11-27
  • Inhibitors of β-Lactamases. New Life of β-Lactam Antibiotics
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    A. M. Egorov, M. M. Ulyashova, M. Yu. Rubtsova

    Abstract β-Lactam antibiotics account for about 60% of all produced antibiotics. Due to a high activity and minimal side effects, they are the most commonly used class of antibacterial drugs for the treatment of various infectious diseases of humans and animals, including severe hospital infections. However, the emergence of bacteria resistant to β-lactams has led to the clinical inefficiency of these

    更新日期:2020-11-27
  • Peculiarities in Activation of Hydrolytic Activity of Elongation Factors
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    A. Paleskava, M. Yu. Kaiumov, S. V. Kirillov, A. L. Konevega

    Abstract Translational GTPases (trGTPases) belong to the family of G proteins and play key roles at all stages of protein biosynthesis on the ribosome. Unidirectional and cyclic functioning of G proteins is ensured by their ability to switch between the active and inactive states due to GTP hydrolysis accelerated by the auxiliary GTPase-activating proteins. Although trGTPases interact with the ribosomes

    更新日期:2020-11-27
  • Antibiotics from Marine Bacteria
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    V. A. Stonik, T. N. Makarieva, L. K. Shubina

    Abstract This review discusses main directions and results of the studies on antibiotics produced by bacteria living in the marine environment. In recent years many obligate marine species and strains were studied, diverse metabolites were isolated, and their chemical structures were elucidated. Among them here were natural compounds toxic against tumor cells, pathogenic bacteria, viruses, and malaria

    更新日期:2020-11-27
  • Inhibition of Chlamydial Infection by CRISPR/Cas9-SAM Mediated Enhancement of Human Peptidoglycan Recognition Proteins Gene Expression in HeLa Cells
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    P. A. Bobrovsky, V. D. Moroz, V. N. Lavrenova, V. A. Manuvera, V. N. Lazarev

    Abstract The global problem of emerging resistance of microorganisms to antibiotics makes the search for new natural substances with antibacterial properties relevant. Such substances include peptidoglycan recognition proteins (PGLYRP), which are the components of the innate immunity of many organisms, including humans. These proteins have a unique mechanism of action that allows them to evade the

    更新日期:2020-11-27
  • rRNA Methylation and Antibiotic Resistance
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    I. A. Osterman, O. A. Dontsova, P. V. Sergiev

    Abstract Methylation of nucleotides in rRNA is one of the basic mechanisms of bacterial resistance to protein synthesis inhibitors. The genes for corresponding methyltransferases have been found in producer strains and clinical isolates of pathogenic bacteria. In some cases, rRNA methylation by housekeeping enzymes is, on the contrary, required for the action of antibiotics. The effects of rRNA modifications

    更新日期:2020-11-27
  • Hibernation as a Stage of Ribosome Functioning
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    K. S. Usachev, M. M. Yusupov, Sh. Z. Validov

    Abstract In response to stress, eubacteria reduce the level of protein synthesis and either disassemble ribosomes into the 30S and 50S subunits or turn them into translationally inactive 70S and 100S complexes. This helps the cell to solve two principal tasks: (i) to reduce the cost of protein biosynthesis under unfavorable conditions, and (ii) to preserve functional ribosomes for rapid recovery of

    更新日期:2020-11-27
  • Next-Generation Antibiotics, Bacteriophage Endolysins, and Nanomaterials for Combating Pathogens
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    I. G. Shemyakin, V. V. Firstova, N. K. Fursova, I. V. Abaev, S. Yu. Filippovich, S. G. Ignatov, I. A. Dyatlov

    Abstract This review presents various strategies to fight causative agents of infectious diseases. Species-specific programmable RNA-containing antibiotics open up new possibilities for creating next-generation of personalized drugs based on microbiome editing and can serve as a new tool for selective elimination of pathogenic bacterial species while keeping intact the rest of microbiota. Another promising

    更新日期:2020-11-27
  • Engineering Artificial Biodiversity of Lantibiotics to Expand Chemical Space of DNA-Encoded Antibiotics
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    S. O. Pipiya, S. S. Terekhov, Yu. A. Mokrushina, V. D. Knorre, I. V. Smirnov, A. G. Gabibov

    Abstract The discovery of antibiotics was one of the fundamental stages in the development of humanity, leading to a dramatic increase in the life expectancy of millions of people all over the world. The uncontrolled use of antibiotics resulted in the selection of resistant strains of bacteria, limiting the effectiveness of antimicrobial therapy nowadays. Antimicrobial peptides (AMPs) were considered

    更新日期:2020-11-27
  • Interaction of Chloramphenicol Cationic Peptide Analogues with the Ribosome
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    Z. Z. Khairullina, A. G. Tereshchenkov, S. A. Zavyalova, E. S. Komarova, D. A. Lukianov, V. N. Tashlitsky, I. A. Osterman, N. V. Sumbatyan

    Abstract Virtual screening of all possible tripeptide analogues of chloramphenicol was performed using molecular docking to evaluate their affinity to bacterial ribosomes. Chloramphenicol analogues that demonstrated the lowest calculated energy of interaction with ribosomes were synthesized. Chloramphenicol amine (CAM) derivatives, which contained specific peptide fragments from the proline-rich antimicrobial

    更新日期:2020-11-27
  • A Quick Guide to Small-Molecule Inhibitors of Eukaryotic Protein Synthesis
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    S. E. Dmitriev, D. O. Vladimirov, K. A. Lashkevich

    Abstract Eukaryotic ribosome and cap-dependent translation are attractive targets in the antitumor, antiviral, anti-inflammatory, and antiparasitic therapies. Currently, a broad array of small-molecule drugs is known that specifically inhibit protein synthesis in eukaryotic cells. Many of them are well-studied ribosome-targeting antibiotics that block translocation, the peptidyl transferase center

    更新日期:2020-11-27
  • Investigation of Allosteric Effect of 2,8-Dimethylation of A2503 in E. coli 23S rRNA by Molecular-Dynamics Simulations
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-11-26
    T. M. Makarova, G. I. Makarov

    Abstract Ribosome is a molecular machine that synthesizes all cellular proteins. It also is a target of about half of the clinically used antibiotics. Adaptive chemical modification of ribosomal RNAs residues is one of the ways to provide resistance to certain antibiotics. A curious example of such modification is 2,8-dimethylation of A2503 in 23S rRNA, which induces resistance to phenols, linkosamides

    更新日期:2020-11-27
  • Erratum to: Bacterial and Protozoan Lipoxygenases Could be Involved in Cell-to-Cell Signaling and Immune Response Suppression
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    G. F. Kurakin, A. M. Samoukina, N. A. Potapova

    On p. 1051, Fig. 2, legend: instead of “nor” should read “not”. On p. 1053, Fig. 4, legend: instead of “initial” should read “original”. On p. 1059, Fig. 7, legend: instead of “initial” should read “original”.

    更新日期:2020-10-30
  • Transglutaminase Type 2 is Involved in the Hematopoietic Stem Cells Homeostasis
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    S. Oliverio, J. S. O. Beltran, L. Occhigrossi, V. Bordoni, C. Agrati, M. D’Eletto, F. Rossin, P. Borelli, G. P. Amarante-Mendes, O. Demidov, N. A. Barlev, M. Piacentini

    Abstract Type 2 transglutaminase (TG2) is a multifunctional protein involved in various biological processes playing a key regulatory role in cell homeostasis such as cell death and autophagy. New evidence is emerging that support an important role of autophagy in regulating normal hematopoiesis. Prompted by these findings, in this study we investigated in vivo involvement of TG2 in mouse hematopoiesis

    更新日期:2020-10-30
  • BNIP3 as a Regulator of Cisplatin-Induced Apoptosis
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    A. S. Gorbunova, T. V. Denisenko, M. A. Yapryntseva, A. D. Pivnyuk, T. A. Prikazchikova, V. G. Gogvadze, B. Zhivotovsky

    Abstract BNIP3 is a member of Bcl-2 protein family involved in regulation of various forms of cell death. However, its role in these processes remains unclear and varies depending on the type of cancer cells and environmental factors (pH, O2 level, etc.). Here, the role of BNIP3 in apoptosis regulation in lung adenocarcinoma cells was investigated. The suppressed expression of BNIP3 caused inhibition

    更新日期:2020-10-30
  • Targeting Bcl-2 Family Proteins: What, Where, When?
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    V. V. Senichkin, N. V. Pervushin, A. P. Zuev, B. Zhivotovsky, G. S. Kopeina

    Abstract Proteins of the Bcl-2 family are known as regulators of apoptosis, one of the most studied forms of programmed cell death. The Bcl-2 protein family is represented by both pro- and antiapoptotic members. Antiapoptotic proteins are often exploited by tumor cells to avoid their death, thus playing an important role in carcinogenesis and in acquisition of resistance to various therapeutic agents

    更新日期:2020-10-30
  • Replicative Senescence and Expression of Autophagy Genes in Mesenchymal Stromal Cells
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    A. Y. Ratushnyy, Y. V. Rudimova, L. B. Buravkova

    Abstract Cell senescence leads to a number of changes in the properties of mesenchymal stromal cells (MSCs). In particular, the number of damaged structures is increased producing negative effect on intracellular processes. Elimination of the damaged molecules and organelles occurs via autophagy that can be important in the context of aging. Cultivation under low oxygen level can be used as an approach

    更新日期:2020-10-30
  • Programmed Cell Death: Historical Notes from Russia
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    B. Zhivotovsky

    Abstract The investigation of cell death mechanisms is one of the fastest growing areas of modern biomedicine. A particular interest in this research topic arose in 1972 after publication of an article by Kerr, Wyllie, and Currie, in which apoptosis, one of the types of cell death, was first considered as a basic biological phenomenon regulating tissue homeostasis. Several Russian groups involved in

    更新日期:2020-10-30
  • The Problem of Apoptotic Processes Reversibility
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    I. I. Zakharov, M. A. Savitskaya, G. E. Onishchenko

    Abstract Apoptosis is the best understood variant of regulated cell death, which has been considered irreversible for a long time. To date, an increasing amount of data has been accumulating indicating that key events of apoptosis, such as the externalization of phosphatidylserine, mitochondrial outer membrane permeabilization, caspase activation, DNA damage, and cytoplasmic blebbing are not irreversible

    更新日期:2020-10-30
  • Reactive Oxygen Species and Antioxidants in Carcinogenesis and Tumor Therapy
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    S. M. Vostrikova, A. B. Grinev, V. G. Gogvadze

    Abstract Strictly regulated balance between the formation and utilization of reactive oxygen species (ROS) is the basis of normal functioning of organisms. ROS play an important role in the regulation of many metabolic processes; however, excessive content of ROS leads to the development of various disorders, including oncological diseases, as a result of ROS-induced mutations in DNA. In tumors, high

    更新日期:2020-10-30
  • NETosis: Molecular Mechanisms, Role in Physiology and Pathology
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    N. V. Vorobjeva, B. V. Chernyak

    Abstract NETosis is a program for formation of neutrophil extracellular traps (NETs), which consist of modified chromatin decorated with bactericidal proteins from granules and cytoplasm. Various pathogens, antibodies and immune complexes, cytokines, microcrystals, and other physiological stimuli can cause NETosis. Induction of NETosis depends on reactive oxygen species (ROS), the main source of which

    更新日期:2020-10-30
  • Mathematical Modeling Reveals the Importance of the DED Filament Composition in the Effects of Small Molecules Targeting Caspase-8/c-FLIP L Heterodimer
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    N. V. Ivanisenko, I. N. Lavrik

    Abstract Procaspase-8 activation at the death-inducing signaling complex (DISC) triggers extrinsic apoptotic pathway. Procaspase-8 activation takes place in the death effector domain (DED) filaments and is regulated by c-FLIP proteins, in particular, by the long isoform c-FLIPL. Recently, the first-in-class chemical probe targeting the caspase-8/c-FLIPL heterodimer was reported. This rationally designed

    更新日期:2020-10-30
  • Programmed Cell Death and Functional Activity of Platelets in Case of Oncohematologic Diseases
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    A. A. Martyanov, A. A. Ignatova, G. S. Svidelskaya, E. A. Ponomarenko, S. P. Gambaryan, A. N. Sveshnikova, M. A. Panteleev

    Abstract Programmed cell death of non-nucleated blood cells – platelets – could be associated with pathophysiology of oncologic and oncohematologic diseases. It contributes to both bleedings (caused by the thrombocytopenia, which is induced by elimination of the platelets) and thrombosis (caused by the processes of blood coagulation on the surface of phosphatidylserine exposing platelets). Here we

    更新日期:2020-10-30
  • Loss of Concurrent Regulation of the Expression of BIF-1, BAX, and Beclin-1 in Primary and Metastatic Melanoma
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    Ž. Frangež, S. M. Seyed Jafari, R. E. Hunger, H.-U. Simon

    Abstract Melanoma is one of the most aggressive and drug-resistant cancers. Despite novel promising therapeutic strategies, the prognosis of metastatic melanoma patients remains poor and it is often associated with high relapse rates. Endophilin B1, also known as BIF-1, is a multifunctional protein involved in several biological processes such as autophagy and apoptosis. BIF-1 promotes apoptosis through

    更新日期:2020-10-30
  • Viral Infections: Negative Regulators of Apoptosis and Oncogenic Factors
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    A. V. Zamaraev, B. Zhivotovsky, G. S. Kopeina

    Abstract The disruption of apoptotic cell death process is closely associated with the etiology of various diseases, including cancer. Permanent viral infections can cause different types of cancers. Oncogenic viruses manipulate both external and internal apoptosis pathways, and inhibit the activity of proapoptotic proteins and signaling pathways, which facilitates carcinogenesis. Ineffective immune

    更新日期:2020-10-30
  • The Role of ERBB2/HER2 Tyrosine Kinase Receptor in the Regulation of Cell Death
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    A. A. Daks, O. A. Fedorova, O. Y. Shuvalov, S. E. Parfenev, N. A. Barlev

    Abstract HER2 (Human Epidermal Growth Factor Receptor 2), also known as ERBB2, CD340, and Neu protooncogene, is a member of the epidermal growth factor receptor (EGRF) family. Members of the ERBB family, including HER2, activate molecular cascades that stimulate proliferation and migration of cancer cells, as well as their resistance to the anticancer therapy. These proteins are often overexpressed

    更新日期:2020-10-30
  • Nutrient Deprivation Promotes MCL-1 Degradation in an Autophagy-Independent Manner
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    N. V. Pervushin, V. V. Senichkin, A. A. Kapusta, A. S. Gorbunova, V. O. Kaminskyy, B. Zhivotovsky, G. S. Kopeina

    Abstract The antiapoptotic protein Mcl-1, which is an attractive target for cancer treatment, is degraded under nutrient deprivation conditions in different types of cancer. This process sensitizes cancer cells to chemotherapy. It has been found that nutrient deprivation leads to suppression of Mcl-1 synthesis; however, the mechanisms of Mcl-1 degradation under such conditions remain to be elucidated

    更新日期:2020-10-30
  • Molecular Mechanisms and Function of the p53 Protein Family Member – p73
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-10-27
    G. Melino

    Abstract Over 20 years after identification of p53 and its crucial function in cancer progression, two members of the same protein family were identified, namely p63 and p73. Since then, a body of information has been accumulated on each of these genes and their interrelations. Biological role of p73 has been elucidated thanks to four distinct knockout mice models: (i) with deletion of the entire TP73

    更新日期:2020-10-30
  • Epidermal Growth Factor Receptor: Key to Selective Intracellular Delivery
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    A. A. Rosenkranz, T. A. Slastnikova

    Abstract Epidermal growth factor receptor (EGFR) is an integral surface protein mediating cellular response to a number of growth factors. Its overexpression and increased activation due to mutations is one of the most common traits of many types of cancer. Development and clinical use of the agents, which block EGFR activation, became a prime example of the personalized targeted medicine. However

    更新日期:2020-09-23
  • CD44-Associated Tn Antigen as a New Biomarker of Tumor Cells with Aberrant Glycosylation
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    M. L. Shuvalova, A. T. Kopylov, D. V. Mazurov, A. V. Pichugin, N. V. Bovin, A. V. Filatov

    Abstract Tn antigen is a tumor-associated antigen that appears on cancer cells as a result of aberrant O-glycosylation. The most studied form of Tn antigen is found in mucins, in particular, in mucin 1 (MUC1). Antibodies against this form of Tn antigen are used to diagnose tumors, as well as to generate T-killers with a chimeric receptor. Some carcinomas do not carry MUC1 and antibodies of a different

    更新日期:2020-09-23
  • Epigenetic Regulation as a Basis for Long-Term Changes in the Nervous System: In Search of Specificity Mechanisms
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    A. A. Borodinova, P. M. Balaban

    Abstract Adaptive long-term changes in the functioning of nervous system (plasticity, memory) are not written in the genome, but are directly associated with the changes in expression of many genes comprising epigenetic regulation. Summarizing the known data regarding the role of epigenetics in regulation of plasticity and memory, we would like to highlight several key aspects. (i) Different chromatin

    更新日期:2020-09-23
  • Flaxseed Cysteine Protease Exhibits Strong Anticoagulant, Antiplatelet, and Clot-Dissolving Properties
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    S. K. M. Nandish, J. Kengaiah, Ch. Ramachandraiah, Chandramma, A. Shivaiah, S. M. Santhosh, Thirunavukkarasu, D. Sannaningaiah

    Abstract In this study, we purified and characterized flaxseed cysteine protease (FSCP) with strong anticoagulant, antiplatelet, and clot-dissolving properties. The enzyme was purified to homogeneity by a combination of gel permeation and ion-exchange column chromatography techniques. The purity of the enzyme was evaluated by SDS-PAGE, RP-HPLC, and MALDI-TOF. FSCP was observed as a single band of approximately

    更新日期:2020-09-23
  • Divergent Impact of Actin Isoforms on Division of Epithelial Cells
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    G. S. Shagieva, I. B. Alieva, C. Chaponnier, V. B. Dugina

    Abstract We investigated distribution and functions of beta- and gamma-cytoplasmic actins (CYAs) at different stages of non-neoplastic epithelial cell division using laser scanning microscopy (LSM). Here, we demonstrated that beta- and gamma-CYAs are spatially segregated in the early prophase, anaphase, telophase, and cytokinesis. Small interfering RNA (siRNA) experiments revealed that in both beta-CYA-

    更新日期:2020-09-23
  • Amyloid and Amyloid-Like Aggregates: Diversity and the Term Crisis
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    A. B. Matiiv, N. P. Trubitsina, A. G. Matveenko, Y. A. Barbitoff, G. A. Zhouravleva, S. A. Bondarev

    Abstract Active accumulation of the data on new amyloids continuing nowadays dissolves boundaries of the term “amyloid”. Currently, it is most often used to designate aggregates with cross-β structure. At the same time, amyloids also exhibit a number of other unusual properties, such as: detergent and protease resistance, interaction with specific dyes, and ability to induce transition of some proteins

    更新日期:2020-09-23
  • Ultrastructure of Hepatocytes from Laboratory Mice Fed a Standard Dry Laboratory Animal Diet
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    V. B. Vays, I. M. Vangeli, O. A. Averina, M. L. Lovat, L. E. Bakeeva

    Abstract The significant destructive changes in ultrastructure of hepatocytes from laboratory mice kept in different vivariums in Moscow and fed with dry laboratory animal diets acquired from different domestic manufacturers that were not standardized for initial products were demonstrated using electron microscopy. Furthermore, disruption in the ultrastructure of liver parenchymal cells occurred regardless

    更新日期:2020-09-23
  • Cell Reprogramming Preserving Epigenetic Age: Advantages and Limitations
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    E. M. Samoylova, V. P. Baklaushev

    Abstract Our understanding of cell aging advanced significantly since the discovery of this phenomenon by Hayflick and Moorhead in 1961. In addition to the well-known shortening of telomeric regions of chromosomes, cell aging is closely associated with changes of the DNA methylation profile. Establishing, maintaining, or reversing epigenetic age of a cell is central to the technology of cell reprogramming

    更新日期:2020-09-23
  • History of Early Bacteriophage Research and Emergence of Key Concepts in Virology
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    A. V. Letarov

    Abstract The viruses of bacteria – bacteriophages – were discovered 20 years after the discovery of viruses. However, this was mainly the bacteriophage research that, after the first 40 years, yielded the modern concept of the virus and to large extent formed the grounds of the emerging molecular genetics and molecular biology. Many specific aspects of the bacteriophage research history have been addressed

    更新日期:2020-09-23
  • Bacterial and Protozoan Lipoxygenases Could be Involved in Cell-to-Cell Signaling and Immune Response Suppression
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-09-22
    G. F. Kurakin, A. M. Samoukina, N. A. Potapova

    Abstract Lipoxygenases are found in animals, plants, and fungi, where they are involved in a wide range of cell-to-cell signaling processes. The presence of lipoxygenases in a number of bacteria and protozoa has been also established, but their biological significance remains poorly understood. Several hypothetical functions of lipoxygenases in bacteria and protozoa have been suggested without experimental

    更新日期:2020-09-23
  • Erratum to: Phycoerythrin Association with Photosystem II in the Cryptophyte Alga Rhodomonas salina
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-08-10
    I. N. Stadnichuk, T. M. Novikova, G. S. Miniuk, V. A. Boichenko, Yu. V. Bolychevtseva, E. S. Gusev, E. P. Lukashev

    On p. 679 in the list of authors and affiliations instead of: 1 Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127726 Moscow, Russia 2 Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia 3 Institute of Fundamental Problems of Biology of the Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia 4

    更新日期:2020-08-11
  • Glutathione Synthesis in Cancer Cells
    Biochemistry Moscow (IF 1.978) Pub Date : 2020-08-10
    E. V. Kalinina, L. A. Gavriliuk

    Abstract Tripeptide GSH is associated not only with the control and maintenance of redox cell homeostasis, but also with the processes of detoxification, proliferation, cell differentiation, and regulation of cell death. Disruptions in GSH synthesis and changes in the GSH/GSSG ratio are common for many pathological conditions, including malignant neoplasms. Numerous data indicate the importance of

    更新日期:2020-08-11
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