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  • Improved operational stability of mediated glucose enzyme electrodes for operation in human physiological solutions
    Bioelectrochemistry (IF 4.474) Pub Date : 2020-01-21
    Richard Bennett; Dónal Leech

    Stability of glucose-oxidising enzyme electrodes is affected by substances in physiological solutions, hampering deployment as long-term implantable biosensors or fuel cells. The performance of Nafion over-coated enzyme electrodes, consisting of multiwalled carbon nanotubes and flavin adenine dinucleotide-dependent glucose dehydrogenase (FADGDH) or glucose oxidase (GOx) crosslinked with osmium-complex based redox polymer, was compared to uncoated electrodes in presence of uric acid and artificial plasma. Nafion over-coating resulted in lower glucose oxidation current densities compared to no over-coating. The highest initial current density for Nafion over-coated electrodes in artificial plasma in 100 mM glucose was 8.0 ± 2.0 mA cm-2 for GOx electrodes with 0.5 % w/v Nafion coating. These electrodes retained 83 % of initial current after 12 hours continuous operation in artificial plasma while similarly prepared FADGDH electrodes retained 58% signal. This is compared to retention of only 73 % or 31 % observed for GOx or FADGDH electrodes in artificial plasma with no Nafion membrane. Enzyme electrodes over-coated with Nafion maintain improved signal stability when tested continuously in the presence of uric acid, identified as being the main contributing substance to FADGDH enzyme electrode instability, showing promise for application to continuous use glucose-oxidising enzyme electrodes.

    更新日期:2020-01-22
  • Long-term bio-power of ceramic Microbial Fuel Cells in individual and stacked configurations
    Bioelectrochemistry (IF 4.474) Pub Date : 2020-01-17
    Iwona Gajda; Oluwatosin Obata; Maria Jose Salar-Garcia; John Greenman; Ioannis A. Ieropoulos
    更新日期:2020-01-17
  • Zinc oxide-gold nanocomposite as a proper platform for label-free DNA biosensor
    Bioelectrochemistry (IF 4.474) Pub Date : 2020-01-16
    Zahra Hatami; Elham Ragheb; Fahimeh Jalali; Mahmoud Amouzadeh Tabrizi; Mojtaba Shamsipur

    In this study, a simple and cost-effective electrochemical DNA biosensor was developed for sensitive detection of mycobacterium tuberculosis (TB). Nanocomposite of zinc oxide (ZnO) and gold nanoparticles (AuNPs) was used as a platform for immobilizing thiolated TB DNA (probe DNA). ZnO was electrodeposited on a glassy carbon electrode by potentiostat electrolysis of Zn (NO3)2 solution at -1.0 V (vs. Ag/AgCl), then AuNPs were loaded as the second layer at -0.4 V from HAuCl4 solution. Thiolated probe DNA was then covalently attached to AuNPs. Anodic peak current of Fe (CN)6 3-/4- was followed in hybridization experiments and a linear calibration curve was obtained in concentration range of 2.5–250 pM and limit of detection (LOD) of 1.8 pM for target DNA. The label-free TB biosensor exhibited high selectivity, suitable stability, and reproducibility.

    更新日期:2020-01-17
  • Infrageneric phylogenetics investigation of Chimonanthus based on electroactive compound profiles
    Bioelectrochemistry (IF 4.474) Pub Date : 2020-01-15
    Yuting Xu; Yijun Lu; Pengchong Zhang; Yangyang Wang; Yuhong Zheng; Li Fu; Huaiwei Zhang; Cheng-Te Lin; Aimin Yu

    Voltammetric scan can record the profile of electrochemical active substances in plant tissues. Because the distribution of chemical components in plants is controlled by genes, these profiles can reflect differences at the genetic level in different species. In this study, the voltammetric scan was applied to the investigation of macrophanerophytes taxonomy. All species of Chimonanthus with two exotaxa were deliberately selected due to their controversial infrageneric relationship. Electrode surface modification was excluded in this work to improve the convenience and accuracy of the fingerprint recording process. The dendrogram deduced from the electrochemical fingerprint data suggests that Ch. zhejiangensis and Ch. grammatus are two groups of Ch. nitens, which may be only the ecotype of Ch. nitens, rather than independent taxonomic species. The small variations between the three species may be due to environmental factors and cannot be used for species formation. In addition, Ch. campanulatus and Ch. praecox were clustered together with a close relationship.

    更新日期:2020-01-15
  • Direct electron transfer-type bioelectrocatalysis of FAD-dependent glucose dehydrogenase using porous gold electrodes and enzymatically implanted platinum nanoclusters
    Bioelectrochemistry (IF 4.474) Pub Date : 2020-01-11
    Taiki Adachi; Takahiro Fujii; Michinari Honda; Yuki Kitazumi; Osamu Shirai; Kenji Kano

    The direct electron transfer (DET)-type bioelectrocatalysis of flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (GDH) from Aspergillus terreus (AtGDH) was carried out using porous gold (Au) electrodes and enzymatically implanted platinum nanoclusters (PtNCs). The porous Au electrodes were prepared by anodization of planar Au electrodes in a phosphate buffer containing glucose as a reductant. Moreover, PtNCs were generated into AtGDH by an enzymatic reduction of hexachloroplatinate (IV) ion. The modification was confirmed by native polyacrylamide gel electrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis analyses. The AtGDH-adsorbed porous Au electrode showed a DET-type bioelectrocatalytic wave both in the presence and absence of PtNCs; however, the current density with PtNCs (∼1 mA cm−2 at 0 V vs. Ag|AgCl|sat. KCl) was considerably higher than that without PtNCs. The kinetic and thermodynamic analysis of the steady-state catalytic wave indicated that inner PtNCs shortened the distance between the catalytic center of AtGDH (= FAD) and the conductive material, and improved the heterogeneous electron transfer kinetics between them.

    更新日期:2020-01-11
  • Highly efficient nitrate reduction driven by an electrocoagulation system: An electrochemical and molecular mechanism
    Bioelectrochemistry (IF 4.474) Pub Date : 2020-01-09
    Mingrui Sui; Yue Dong; Peifang Wang; Yuhang Zhang; Xiao Tan; Yi Li
    更新日期:2020-01-09
  • Development of an Escherichia coli-based Electrochemical Biosensor for Mycotoxin Toxicity Detection
    Bioelectrochemistry (IF 4.474) Pub Date : 2020-01-09
    Yafei Chen; Yajie Yang; Yu Wang; Ye Peng; Jinmei Nie; Guanyue Gao; Jinfang Zhi

    Mycotoxin contamination in food and feed is a global concern because mycotoxin contamination can cause both acute and chronic health effects in humans and animals. In the present work, an Escherichia coli-based biosensor is described for the toxicity assessment of aflatoxin B1 (AFB1) and zearalenone (ZEN). In this electrochemical biosensor, E. coli is used as the signal recognition element, p-benzoquinone is used as the mediator, and a two-step reaction procedure has been developed to separate the mediator from the mycotoxins. The current value of the as-prepared microbial biosensor exhibits a linear decrease with concentrations of AFB1 and ZEN in the range of 0.01-0.3 and 0.05-0.5 μg/mL, with detection limits reaching 1 and 6 ng/mL, respectively. The IC25 values obtained by the present method are 0.25 and 0.40 μg/mL for AFB1 and ZEN, which shows that the cytotoxicity of AFB1 to E. coli is more severe than the cytotoxicity of ZEN to E. coli. The combined toxic effect of these two mycotoxins has also been explored, and synergistic biotoxicity has been observed. Moreover, the biosensor is successfully applied to the toxicity evaluation of mycotoxins in real samples, including peanut and corn oils. This work could provide new insight into mycotoxin and microorganism interactions and could establish a new approach for future mycotoxin detection.

    更新日期:2020-01-09
  • Structural and electrochemical characterization of lawsone-dependent production of tellurium-metal nanoprecipitates by photosynthetic cells of Rhodobacter capsulatus
    Bioelectrochemistry (IF 4.474) Pub Date : 2020-01-08
    Roberto Borghese; Marco Malferrari; Marco Brucale; Luca Ortolani; Martina Franchini; Stefania Rapino; Francesca Borsetti; Davide Zannoni
    更新日期:2020-01-09
  • A non-contact impedimetric biosensing system for classification of toxins associated with cytotoxicity testing
    Bioelectrochemistry (IF 4.474) Pub Date : 2020-01-07
    Angelines Gasser; John Eveness; Janice Kiely; David Attwood; Richard Luxton

    We report on a novel impedance spectroscopy measurement and data analysis technique for cytotoxicity testing. The technique combines non-contact measurement with real-time impedance data analysis based on the toxin dose dependency of the outputs, making it suitable for high throughput screening. A multi-electrode array was designed and fabricated such that a standard well plate could be positioned above the electrodes, negating the requirement for bespoke culture wells with integrated electrodes. For cytotoxicity testing, endothelial cells, type ECV304, within the wells were exposed to various concentrations of 3 toxins, dimethyl sulphoxide, cadmium chloride and saponin, which exhibit different modes of action on cells. Impedance spectra were recorded every 30 minutes over a 24 hour period. From the spectra ‘toxin maps’ were produced which presented the correlation between impedance output and dose of toxin versus frequency and time. The results demonstrated characteristic toxin maps for each toxin and significantly differences between the three toxins studied. Using complementary measurement methods, we showed that these differences in toxin maps related to morphological and physiological changes in the cells due to the differing mode of action of each toxin.

    更新日期:2020-01-07
  • 更新日期:2020-01-02
  • 更新日期:2020-01-01
  • Luminal addition of non-permeant Eu3+ interferes with luminal Ca2+ regulation of the cardiac ryanodine receptor
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-28
    Jana Gaburjakova; Janos Almassy; Marta Gaburjakova
    更新日期:2019-12-29
  • Study of an Antarctic thermophilic consortium and its influence on the electrochemical behavior of aluminum alloy 7075-T6
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-28
    Joaquín Atalah; Lotsé Blamey; Hans Köhler; Ilda M. Alfaro-Valdéz; Carlos Galarce; Claudia Alvarado; Mamié Sancy; Maritza Páez; Jenny M. Blamey
    更新日期:2019-12-29
  • Cancellation effect is present in high-frequency reversible and irreversible electroporation
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-24
    Tamara Polajžer; Janja Dermol–Černe; Matej Reberšek; Rodney ÓConnor; Damijan Miklavčič

    It was recently suggested that applying high-frequency short biphasic pulses (HF-IRE) reduces pain and muscle contractions in electrochemotherapy and irreversible ablation treatments; however, higher amplitudes with HF-IRE pulses are required to achieve a similar effect as with monophasic pulses. HF-IRE pulses are in the range of a microseconds, thus, the so-called cancellation effect could be responsible for the need to apply pulses of higher amplitudes. In cancellation effect, the effect of first pulse is reduced by the second pulse of opposite polarity. We evaluated cancellation effect with high-frequency biphasic pulses on CHO-K1 in different electroporation buffers. We applied eight bursts of 1 – 10 µs long pulses with inter-phase delays of 0.5 µs – 10 ms and evaluated membrane permeability and cell survival. In permeability experiments, cancellation effect was not observed in low-conductivity buffer. Cancellation effect was, however, observed in treatments with high-frequency biphasic pulses looking at survival in all of the tested electroporation buffers. In general, cancellation effect depended on inter-phase delay as well as on pulse duration, i.e. longer pulses and longer interphase delay cause less pronounced cancellation effect. Cancellation effect could be partially explained by the assisted discharge and not by the hyperpolarization by the chloride channels.

    更新日期:2019-12-25
  • Electrochemical detection of specific interaction between apolipoprotein E isoforms and DNA sequences related to Alzheimer’s disease
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-23
    Dhésmon Lima; Ana Carolina M. Hacke; Juliana Inaba; Christiana A. Pessôa; Kagan Kerman

    Apolipoprotein E4 (ApoE4) has a key role on the onset and progression of Alzheimer’s disease (AD), since it favours the deposition of toxic amyloid-beta (Aβ) aggregates in the brain. These effects might result from the interaction between ApoE4 and specific DNA promoters related to cellular autophagy pathways and to the expression of neuroprotective proteins, like sirtuin-1 (SirT1). Herein, we modified gold electrodes with mixed self-assembled monolayer of 6-mercapto-1-hexanol and thiolated DNA oligonucleotides related to CLEAR (associated with autophagic processes that enable the clearance of toxic species, such as Aβ) and SirT1 promoter. The interactions of the immobilized DNA with isoforms of ApoE (ApoE4/ApoE3/ApoE2) were investigated with differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). By monitoring current and charge transfer resistance (Rct) variations, CLEAR showed to interact specifically with ApoE4, whereas SirT1 showed a higher affinity to ApoE4 compared to ApoE3 and ApoE2. To the best of our knowledge, this is the first report about the application of electrochemical techniques to investigate the sequence-specific interaction between ApoE isoforms and CLEAR and SirT1 oligonucleotides.

    更新日期:2019-12-23
  • Differences in cellular damages induced by dielectric barrier discharge plasma between Salmonella Typhimurium and Staphylococcus aureus
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-18
    Mingming Huang; Hong Zhuang; Jianying Zhao; Jiamei Wang; Wenjing Yan; Jianhao Zhang

    Dielectric barrier discharge (DBD) plasma treatments are more effective against Gram negative bacteria than Gram-positive bacteria. Effects of DBD plasma were compared on Salmonella Typhimurium and Staphylococcus aureus cells. Physical cell membrane integrity and function, deoxyribonucleic acid (DNA) oxidation, and intracellular reactive oxygen species (ROS) were measured. The difference in physical damage caused by plasma on Gram-negative and Gram positive bacteria implied a difference in cellular damage pattern, which may be due to differences in cell structure and composition. Antimicrobial particles in plasma react directly with the outer membranes of Gram-negative bacteria and eventually kill them; however, ROS produced from plasma first penetrated the cell wall and membrane of Gram-positive bacteria before reacting with internal cellular components and killing the bacteria.

    更新日期:2019-12-19
  • Electropermeabilization does not correlate with plasma membrane lipid oxidation
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-15
    Olga Michel; Andrei G. Pakhomov; Maura Casciola; Jolanta Saczko; Julita Kulbacka; Olga N. Pakhomova
    更新日期:2019-12-17
  • The shape of lipid molecules affects potential-driven molecular-scale rearrangements in model cell membranes on electrodes
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-14
    Bishoy Khairalla; Joanna Juhaniewicz-Debinska; Slawomir Sek; Izabella Brand
    更新日期:2019-12-17
  • Contactless Electroporation Induced by High Intensity Pulsed Electromagnetic Fields via Distributed Nanoelectrodes
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-13
    Damijan Miklavcic; Vitalij Novickij; Matej Kranjc; Tamara Polajzer; Sasa Haberl Meglic; Tina Batista Napotnik; Rok Romih; Darja Lisjak
    更新日期:2019-12-13
  • Efficient and synergistic decolourization and nitrate removal using a single-chamber with a coupled biocathode-photoanode system
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-13
    Shigang Su; Yizhu Zhang; Wenjuan Hu; Xia Zhang; Dianxing Ju; Changchao Jia; Jian Liu

    With the continuous development of the chemical industries, synergistic removal of carbon and nitrogen contaminants has drawn much attention. In this work, a novel strategy for the synergistic removal of methyl orange (MO) and nitrate was developed in a single reactor by combining a TiO2/g-C3N4 nanosheet/graphene photoanode and denitrifying biofilm cathode. Under xenon light illumination, the photocatalytic MO decolorization rate exceeded 90% (the initial concentration of MO was as high as 100 mg·L-1) with a biocathode potential bias of -0.5 V vs Ag/AgCl; additionally, the decolourization rate apparently followed first-order kinetics with a constant of 0.11 ± 0.02 h-1. The improved MO decolourization rate was mainly because the biocathode effectively enhanced the charge separation of the photogenerated charge at the TiO2/g-C3N4 nanosheet/graphene photoanode interface. In the meantime, the effluent nitrate was lower than 1 mg·N·L-1 at a biocathode potential of -0.5 V vs Ag/AgCl. The results indicated that the coupled biocathode-photoanode system could serve the purpose of simultaneously degrading MO and accomplishing nitrate reduction. Considering the sustainability of sunlight and the use of a biocathode, the coupled biocathode-photoanode system is a promising alternative for the simultaneous removal of biorefractory organics and nitrate.

    更新日期:2019-12-13
  • 更新日期:2019-12-13
  • 更新日期:2019-12-13
  • Molecular recognition between guanine and cytidine in a head group of nucleolipid monolayer forming the top leaflet of a hybrid bilayer supported at a gold (111) electrode
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-06
    Julia Alvarez-Malmagro, ZhangFei Su, J. Jay Leitch, Francisco Prieto, Manuela Rueda, Jacek Lipkowski

    A hybrid bilayer lipid membrane (hBLM), composed of a self-assembled, 1-hexadecanethiol monolayer interior leaflet and a 1,2-dipalmitoyl-sn-glycero-3-cytidine nucleolipid monolayer exterior leaflet, was deposited at the surface of a gold (111) electrode. This system was used to investigate the molecular recognition reaction between the cytosine moieties of the lipid head group with guanine molecules present within the bulk electrolyte solution. Electrochemical measurements and photon polarization modulation infrared reflection absorption spectroscopy (PM IRRAS) were employed to characterize the system and determine the extent of the molecular recognition reaction. The capacitance of the hBLM-covered gold electrode was very low (∼1 μF cm-2), therefore the charge density at the gold surface and changes in the static electric field acting on the membrane were small. Changing the electrode potential had a minimal effect on formation of the complex between the cytosine moiety guanine molecule due to the small changes in the static electric field across the membrane. This behavior favored formation of the complex between guanine and cytosine.

    更新日期:2019-12-06
  • Nanosecond Pulses Targeting Intracellular Ablation Increase Destruction of Tumor Cells with Irregular Morphology
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-04
    Chenguo Yao, Junyi Ning, Hongmei Liu, Yanpeng Lv, Yajun Zhao, Shoulong Dong

    The decrease in killing sensitivity of the cell membrane to microsecond pulse electric fields (μs-PEFs) is ascribed mainly to the aberrant morphology of cancer cells, with clear statistical correlations observed between cell size and shape defects and the worsening of the electrical response to the PEF. In this paper, nanosecond pulsed electric fields (ns-PEFs) inducing the nucleus effect and μs-PEFs targeting the cell membrane were combined to enhance destruction of irregular cells. The fluorescence dissipation levels of the nuclear membrane and cell membrane exposed to the μs, ns, and ns+μs pulse protocols were measured and compared, and a dynamic electroporation model of irregular cells was established by the finite element software COMSOL. The results suggest that the cell membrane disruption induced by μs-PEFs is worse for extremely irregular cells and depends strongly on cellular morphology. However, the nuclear membrane disruption induced by ns-PEFs does not scale with irregularity, suggesting the use of a combination of ns-PEFs with μs-PEFs to target the nuclear and cell membranes. We demonstrate that ns+μs pulses can significantly enhance the fluorescence dissipation of the cell and nuclear membranes. Overall, our findings indicate that ns+μs pulses may be useful in the effective killing of irregular cells.

    更新日期:2019-12-04
  • Promoting laccase sensing activity for catechol detection using LBL assemblies of chitosan/ionic liquid/ phthalocyanine as immobilization surfaces
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-04
    C. Salvo-Comino, C. Garcia-Hernandez, C. Garcia-Cabezon, M.L. Rodriguez-Mendez
    更新日期:2019-12-04
  • Flow Micropillar Array Electroporation to Enhance Size Specific Transfection to A Large Population of Cells
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-04
    Yingbo Zu, Xuan Liu, An-Yi Chang, Shengnian Wang

    Despite serving as a popular non-viral delivery approach, electroporation carries several drawbacks in its current configurations. We developed a Flow Micropillar-array Electroporation (FME) system to wisely regulate an important transmembrane-determining factor, namely cell size variations among individual cells, to achieve effective transfection. In FME, cells flow through a slit-type microfluidic channel on which carbon electrodes with well-patterned micropillar array texture are integrated as the top and bottom wall. Gravity helps bring cells to the micropillar array surface so that the permeable area on cells in different size populations is specified by their size regardless their random location fact. Without sacrificing cell viability, we demonstrate this FME concept by delivering DNA plasmids to several mammalian cell lines with obvious transfection enhancement when compared to a commercial system (K562: 3.0 folds; A549: 3.3 folds; HeLa: 1.8 folds, COS7: 1.7 folds; 293T: 2.9 folds; mES: 2.5 folds). Moreover, carbon-based electrodes are less expensive, more durable, and convenient for integration with a microfluidic setup which enables rapid and massive transfection capability that many therapeutic application needs. The success of FME may benefit many emerging biological studies and clinical practice that requires effective transfection to a large population of cells in limited processing time.

    更新日期:2019-12-04
  • 更新日期:2019-12-04
  • 更新日期:2019-12-02
  • Electrocatalytic nanostructured ferric tannate as platform for enzyme conjugation: electrochemical determination of phenolic compounds
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-12-02
    Massimiliano Magro, Davide Baratella, Veronica Colò, Francesca Vallese, Carlo Nicoletto, Silvia Santagata, Paolo Sambo, Simone Molinari, Gabriella Salviulo, Andrea Venerando, Caroline R. Basso, Valber A. Pedrosa, Fabio Vianello

    A shell of nanostructured ferric tannates was spontaneously developed on the surface of naked maghemite nanoparticles (SAMNs, the core) by a simple wet reaction with tannic acid (TA). The as obtained core–shell nanomaterial (SAMN@TA) displays specific electrocatalytic and surface properties, which significantly differ from parent maghemite. Thanks to the known proclivity of TA to interact with proteins, SAMN@TA was proposed as a support for the direct immobilization of an enzyme. A ternary functional nanobioconjugate (SAMN@TA@TvL) was successfully self-assembled by incubating laccase from Trametes versicolor (TvL) and SAMN@TA. The SAMN@TA@TvL hybrid was kinetically characterized with respect to the native enzyme and applied for building an easy-to-use analytical device for the detection of polyphenols. The electrochemical biosensor allowed the determination of polyphenols by square wave voltammetry in mixed water-methanol solutions. The system sensitivity was 868.9 ± 1.9 nA µM-1, the LOD was 81 nM and the linearity range was comprised between 100 nM to 10 µM. The proposed approach was successfully applied to detect phenolics in blueberry extracts as real samples. Results suggest that SAMN@TA could be a promising, low cost and versatile tool for the creation of nano-bio-conjugates aimed at the development of new electrochemical sensing platforms.

    更新日期:2019-12-02
  • Highly uniform in-situ cell electrotransfection of adherent cultures using grouped interdigitated electrodes
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-30
    Yicen Zhou, Ying Lu, Jing Cheng, Youchun Xu

    Cell electrotransfection is an effective approach for transferring exogenous molecules into living cells by electric stimulation. The existing in-situ electrotransfection micro-devices for adherent cells exhibit the drawbacks of low transfection efficiency and low cell viability. An important reason for these drawbacks is the unequal exposure of cells to the electric field. It was found that cells growing directly below the energized electrodes experience a much lower electric field intensity when compared to the cells growing below the spacing area of the electrodes, resulting in low transfection with a strip-like pattern. Therefore, a new strategy for the in-situ electrotransfection of adherent cells growing in a standard 12-well plate is proposed in this study. By sequentially energizing electrodes arranged in a nested and non-contact manner, the cells were exposed to an overall equal intensity of the electric field, and thus a higher efficiency of transfection was achieved. The seven cell lines transfected using this method exhibited high transfection efficiency and high cell viability, demonstrating the potential for studying gene function.

    更新日期:2019-11-30
  • Microwave-assisted preparation of ZnFe2O4-Ag/rGO nanocomposites for amplification signal detection of alpha-fetoprotein
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-30
    Mei Wu, Yuxiao Yang, Kaihang Cao, Chulei Zhao, Xiuwen Qiao, Chenglin Hong
    更新日期:2019-11-30
  • Electrochemical characterization and estimation of DNA-binding capacity of a series of novel ferrocene derivatives
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-30
    Marko Pešić, Jovana Bugarinović, Aleksandra Minić, Slađana Novaković, Goran Bogdanović, Anka Todosijević, Dragana Stevanović, Ivan Damljanović
    更新日期:2019-11-30
  • Employment of electrostriction phenomenon for label-free electrochemical immunosensing of tetracycline
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-29
    Karolina Starzec, Cecilia Cristea, Mihaela Tertis, Bogdan Feier, Marcin Wieczorek, Paweł Kościelniak, Jolanta Kochana
    更新日期:2019-11-30
  • Bioelectrical model of head-tail patterning based on cell ion channels and intercellular gap junctions
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-29
    Javier Cervera, Salvador Meseguer, Michel Levin, Salvador Mafe
    更新日期:2019-11-30
  • Development of a molecularly imprinted polymer electrochemical sensor and its application for sensitive detection and determination of malathion in olive fruits and oils
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-29
    Youssra Aghoutane, Alassane Diouf, Lars Österlund, Benachir Bouchikhi, Nezha El Bari

    Malathion (MAL) is an organophosphorus (OP) insecticide. It is a cholinesterase inhibitor, which can pose serious health and environmental problems. In this study, a sensitive and selective molecular imprinted polymer (MIP) based on screen-printed gold electrodes (Au-SPE) for MAL detection in olive oils and fruits, was devised. The MIP sensor was prepared using acrylamide as the functional monomer and MAL as the template. Subsequently, the morphology of the electrode surface was studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electrochemical characterization of the developed MIP sensor was performed by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) techniques. The operational repeatability and stability of the sensor were studied. It was found to have a dynamic concentration range of (0.1 pg mL-1-1000 pg mL-1) and a low limit of detection (LOD) of 0.06 pg mL-1. Furthermore, the sensor was employed to determine MAL content in olive oil with a recovery rate of 87.9% and a relative standard deviation of 8%. It was successfully applied for MAL determination in real samples and promise to open new opportunities for the detection of OP pesticides residues in various food products, as well as in environmental applications.

    更新日期:2019-11-30
  • Pyranose Oxidase: A Versatile Sugar Oxidoreductase for Bioelectrochemical Applications
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-29
    Annabelle T. Abrera, Leander Sützl, Dietmar Haltrich

    Pyranose oxidase (POx) is an FAD-dependent oxidoreductase, and like glucose oxidase (GOx) it is a member of the glucose-methanol-choline (GMC) superfamily of oxidoreductases. POx oxidizes several monosaccharides including D-glucose, D-galactose, and D-xylose, while concurrently oxygen is reduced to hydrogen peroxide. In addition to this oxidase activity, POx shows pronounced activity with alternative electron acceptors that include various quinones or (complexed) metal ions. Even though POx in general shows properties that are more favourable than those of GOx (e.g., a considerably higher catalytic efficiency (kcat/Km) for D-glucose, significantly lower Michaelis constants Km for D-glucose, reactivity with both anomeric forms of D-glucose) it is much less frequently used for both biosensor and biofuel cell applications than GOx. POx has been applied in biosensing of D-glucose, D-galactose, and D-xylose, and in combination with α-glucosidase also maltose. An attractive application is in biosensors constructed for the measurement of 1,5-anhydro-D-glucitol, a recognised biomarker in diabetes. Bioelectrochemical applications of POx had been restricted to enzymes of fungal origin. The recent discovery and characterisation of POx from bacterial sources, which show properties that are very distinct from the fungal enzymes, might open new possibilities for further applications in bioelectrochemistry.

    更新日期:2019-11-30
  • The protection mechanism offered by Heterophragma adenophyllum extract against Fe-C steel dissolution at low pH: Computational, statistical and electrochemical investigations
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-27
    Priti Pahuja, Nisha Saini, Bhaskaran, Abdelkarim Chaouiki, Rachid Salghi, Sumit Kumar, Suman Lata

    An ethanolic extract of Heterophragma adenophyllum (HA) was investigated as corrosion inhibitor for Fe-C steel in hydrochloric acid. The inhibition effect of HA extract was examined using the weight deterioration, electrochemical impedance spectroscopy (EIS) and polarization techniques. EIS showed enhanced charge transfer resistance with maximum protection value of ∼96% at 600 ppm concentration. Tafel extrapolation results revealed that corrosion was restricted by mixed inhibition. The icorr values reduced considerably from 53.63 μA cm-2 at 100 ppm to 20.11 μA cm-2 at 600 ppm of HA extract. Interaction intensity was further examined through Langmuir, Freundlich, D-R, Frumkin, and Flory-Huggins adsorption isotherms. The computational study, statistical modelling and surface morphology by XPS, AFM, and SEM-EDX, indicated an excellent adsorption capability of HA on Fe-C steel.

    更新日期:2019-11-28
  • Electron donor availability controls scale up of anodic biofilms
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-27
    Secil Tutar, Abdelrhman Mohamed, Phuc T. Ha, Haluk Beyenal

    The scale-up of bioelectrochemical systems (BESs) is a challenging problem that limits the advancement and practical implementation of the coupled technology. The goal of this work is to acquire an understanding of the limitations on scaling up anodic biofilms in BESs. We hypothesized that scaling up is dependent on the availability of electron donors. We tested this hypothesis by enriching anodic biofilms on electrodes of multiple sizes (15 cm2 to 466 cm2) and quantified the anodic current densities while varying the electron donor concentrations. The anodic biofilms were enriched on electrodes under two conditions: 1) in raw wastewater and 2) in wastewater supplemented with 20 mM acetate. Following anodic biofilm enrichment, the current density for each electrode was quantified in artificial wastewater medium with variable COD loadings using acetate as an electron donor. Current generated using anodic biofilms scaled up at a high COD loading (1500 mg/L), while current density decreased with increasing electrode size at lower COD loadings. Further, microbial community analysis revealed that the microbial community was independent of the electrode size but dependent on the medium composition during the enrichment phase. These results provide a practical framework for the design of large-scale BESs based on laboratory-scale measurements.

    更新日期:2019-11-28
  • The effect of start-up on energy recovery and compositional changes in brewery wastewater in bioelectrochemical systems
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-26
    Johanna M. Haavisto, Marika E. Kokko, Aino-Maija Lakaniemi, Mira L.K. Sulonen, Jaakko A. Puhakka
    更新日期:2019-11-27
  • Microbiologically influenced corrosion mechanism of 304L stainless steel in treated urban wastewater and protective effect of silane-TiO2 coating
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-21
    I. Ziadi, M.M. Alves, M. Taryba, L. El-Bassi, H. Hassairi, L. Bousselmi, M.F. Montemor, H. Akrout
    更新日期:2019-11-22
  • FAD dependent glucose dehydrogenases – discovery and engineering of representative glucose sensing enzymes -
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-20
    Junko Okuda-Shimazaki, Hiromi Yoshida, Koji Sode

    The history of the development of glucose sensors goes hand-in-hand with the history of discovery and engineering of glucose-sensing enzymes. Glucose oxidase (GOx) has been used for glucose sensing since the development of the first electrochemical glucose sensor. The principle utilizing oxygen as the electron acceptor is designated as the first-generation electrochemical enzyme sensors. With increasing demand for hand-held and cost-effective devices for the “self-monitoring of blood glucose (SMBG)”, second-generation electrochemical sensor strips employing electron mediators have become the most popular platform. To overcome the inherent drawback of GOx, namely, the use of oxygen as the electron acceptor, various glucose dehydrogenases (GDHs) have been utilized in second-generation principle-based sensors. Among the various enzymes employed in glucose sensors, GDHs harboring FAD as the redox cofactor, FADGDHs, especially those derived from fungi, fFADGDHs, are currently the most popular enzymes in the sensor strips of second-generation SMBG sensors. In addition, the third-generation principle, employing direct electron transfer (DET), is considered the most elegant approach and is ideal for use in electrochemical enzyme sensors. However, glucose oxidoreductases capable of DET are limited. One of the most prominent GDHs capable of DET is a bacteria-derived FADGDH complex (bFADGDH). bFADGDH has three distinct subunits; the FAD harboring the catalytic subunit, the small subunit, and the electron-transfer subunit, which makes bFADGDH capable of DET. In this review, we focused on the two representative glucose sensing enzymes, fFADGDHs and bFADGDHs, by presenting their discovery, sources, and protein and enzyme properties, and the current engineering strategies to improve their potential in sensor applications. (254 words)

    更新日期:2019-11-21
  • Surface Modification of Cerasomes with AuNPs@Poly(Ionic Liquid)s for an Enhanced Stereo Biomimetic Membrane Electrochemical Platform
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-20
    Daliang Liu, Qiong Wu, Shun Zou, Feiyun Bao, Jun-ichi Kikuchi, Xi-Ming Song
    更新日期:2019-11-21
  • Reduced nonspecific protein adsorption by application of diethyldithiocarbamate in receptor layer of diphtheria toxoid electrochemical immunosensor
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-20
    Robert Ziółkowski, Adrianna Kaczmarek, Ilona Kośnik, Elżbieta Malinowska

    The immunoassay technology is of particular importance for both the environmental industry and clinical analysis. Biosensors, with the sensing layer based on antibodies or their fragments, offer high selectivity and short detection times. However, analytical devices where the electrochemical signal corresponds to changes in the interfacial region (sensing layer/electrode surface) are very susceptible to any nonspecific adsorption. Unfortunately, proteins (including antibodies) belong to the molecules showing high non-specific interactions with solid substrates. Herein, we propose diethyldithiocarbamate as a new antifouling and highly conductive agent. The investigations were conducted to evaluate its interaction with chosen proteins and the mechanism of its co-adsorption with biotinylated thiol (an anchor point for immune-sensing elements). The developed receptor layer is characterised by reduced nonspecific protein adsorption and high conductivity with the same preserved specificity of the antibodies (immobilised by the streptavidin/biotin bioaffinity technique). This allowed for selective detection of the diphtheria toxoid, an inactive toxin secreted by virulent strains of Corynebacterium diphtheria, at the level of 5⋅10-6 μg⋅ml-1 (1⋅10-6 Lf⋅ml-1) and in the real-life sample.

    更新日期:2019-11-21
  • 更新日期:2019-11-20
  • Corrosion behavior and mechanism of carbon steel influenced by interior deposit microflora of an in-service pipeline
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-19
    Hong Su, Ruohao Tang, Xiaowei Peng, Aiguo Gao, Yejun Han

    Investigation of carbon steel corrosion influenced by in-situ microbial communities can provide reliable information about microbiologically influenced corrosion (MIC) in the oil and gas field. Here, we investigated the 90-day corrosion behavior of Q235 carbon steel influenced by interior deposit microflora of an in-service pipeline using open circuit potential (OCP) and electrochemical impedance spectroscopy (EIS). Linear sweep voltammetry (LSV), 16S rRNA gene sequencing, and surface analysis were used to comprehensively analyze the corrosion mechanisms. The results indicated that OCP was decreased while the charge transfer resistance (Rct) was increased, and that steel corrosion was inhibited during the first 45 days. Subsequently, OCP was significantly increased while Rct was rapidly decreased, and steel corrosion was enhanced. After 90-day immersion, severe pitting corrosion with a maximum pit depth of 89.6 μm occurred on the steel surface. Viable microbes in the final biofilm significantly increased the cathodic current. Iron carbonate, chukanovite and cementite were identified as the main corrosion products on the steel surface. Methanobacterium dominated the final biofilm community. These observations indicate that the corrosion mechanism of the final biofilm can be explained by extracellular electron transfer MIC in which microbes corrode steel by direct electron uptake.

    更新日期:2019-11-19
  • 更新日期:2019-11-18
  • Molecularly imprinted electrochemical sensor based on polypyrrole/dopamine@graphene incorporated with surface molecularly imprinted polymers thin film for recognition of olaquindox
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-15
    Xiaoyun Bai, Bo Zhang, Miao Liu, Xuelian Hu, Guozhen Fang, Shuo Wang

    In this paper, an advanced molecularly imprinted electrochemical sensor (MIECS) based on electropolymerized olaquindox (OLA) surface molecularly imprinted polymer thin film on a modified glassy carbon electrode (GCE) was developed for the detection of OLA. It was fabricated by coating dopamine@graphene (DGr) on GCE, then electropolymerizing pyrrole (Py) and molecularly imprinted polymers (MIPs). Graphene (Gr) was introduced for improving conductivity and sensitivity. Dopamine (DA) was used for dispersion and adhesion of Gr. Polypyrrole (PPy) could fix DGr and enhance the current response evidently. The established sensor could selectively recognize OLA but not the analogs of OLA. Some essential parameters controlling the performance of the developed sensor were investigated and optimized. Under optimal conditions, the linear relationship between the current intensity and OLA concentration was obtained from 50 nmol L-1 to 500 nmol L-1 with a limit of detection (LOD) of 7.5 nmol L-1. Analytical results of OLA based on the developed MIECS for fish and feedstuffs showed a good agreement with the results based on high performance liquid chromatography (HPLC).

    更新日期:2019-11-15
  • Study of pitting corrosion inhibition effect on aluminum alloy in seawater by biomineralized film
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-11-15
    Yuanyuan Shen, Yaohua Dong, Tao Liu, Zhangwei Guo, Qinghong Li, Lihua Dong, Yansheng Yin, Xiaoxue Wang
    更新日期:2019-11-15
  • 更新日期:2019-10-14
  • Polyclonal Antibody Production Anti Pc_312-324 peptide. Its Potential Use in Electrochemical Immunosensors for Transgenic Soybean Detection
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-10-04
    Marcos E. Farías, Mariela M. Marani, Darío Ramírez, Ana M. Niebylski, N. Mariano Correa, Patricia G. Molina

    A new polyclonal antibody that recognizes the CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS), which provides resistance to glyphosate in soybean (Roundup Ready®, RR soybean), was produced. New Zealand rabbits were injected with a synthetic peptide (Pc_312-324, (PEP)) present in the soybean CP4-EPSPS protein. The anti-PEP antibodies production was evaluated by electrophoresis (SDS-PAGE) and an enzyme-linked immunosorbent assay (ELISA) was developed in order to study their specificity. The ELISA showed that the polyclonal antibody was specific to PEP. In addition, the anti- PEP was immobilized onto a gold disk electrode and the antigen-antibody interaction was evaluated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Moreover, the EIS showed that the electron transfer resistance of the modified electrode increased after incubation with solutions containing CP4-EPSPS protein from RR transgenic soybean, while no changes were detected after incubation with no-RR soybean proteins. These results suggest that the CP4-EPSPS was immobilized onto the electrode, due to the specific interaction with the anti-PEP. These results show that this antigen-antibody interaction can be detected by electrochemical techniques, suggesting that the anti-PEP produced can be used in electrochemical immunosensors development to quantify transgenic soybean.

    更新日期:2019-10-05
  • Fabrication of an electrochemical chiral sensor via an integrated polysaccharides/3D nitrogen-doped graphene-CNT frame
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-10-04
    Xiaohui Niu, Xing Yang, Zunli Mo, Jia Wang, Zhao Pan, Zhenyu Liu, ChaoShuai, Guigui Liu, Nijuan Liu, Ruibin Guo

    We report a novel chiral interface based on polysaccharides that was integrated via an amidation reaction between the -COOH of sodium alginate and the -NH2 of chitosan to form a chiral selector (SA-CS) with three dimensional N-doped graphene-CNT (NGC) as the substrate material. This interface was used for chiral discrimination of tryptophan (Trp) enantiomers via electrochemical measurements. The FT-IR, SEM, TEM and XPS characterization showed that the chiral selector and substrate materials were prepared successfully. Compared with individual SA-CS and NGC, the integrated polysaccharides/3D NGC showed higher enantioselectivity for L-Trp than D-Trp due to the smaller steric hindrance for D-Trp during the formation of three-point interactions between the two diastereoisomeric enantiomer-selector complexes, which allowed L-Trp to more easily detach from the electrode modification layer and approach the electrode surface, facilitating its approach and confirming that SA-CS had a higher constant for L-Trp when applied to real samples.

    更新日期:2019-10-05
  • Effect of electrode spacing on electron transfer and conductivity of Geobacter sulfurreducens biofilms
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-10-04
    Panpan Liu, Abdelrhman Mohamed, Peng Liang, Haluk Beyenal
    更新日期:2019-10-05
  • Discovery of a novel quinohemoprotein from a eukaryote and its application in electrochemical devices
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-09-15
    Kouta Takeda, Kiyohiko Igarashi, Makoto Yoshida, Nobuhumi Nakamura
    更新日期:2019-09-16
  • Evaluation of the biocompatibility of a PVA/SA scaffold with a human gingival fibroblast (HGF) by using electrochemical impedance spectroscopy
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-09-15
    M. Hernández, M.A. Álvarez-Pérez, J. Genesca, K.K. Gómez, A. Covelo

    The biocompatibility of human gingival fibroblasts (HGF) was evaluated in different concentrations of poly(vinyl alcohol) and sodium alginate (PVA/SA) nanofibres (3.5 wt% 4 wt% and 5 wt%). The PVA/SA nanofibres were deposited on the surface of an electrode microchip by using the electrospinning technique. Electrochemical impedance spectroscopy (EIS) was applied to measure the dielectric properties of each system. In order to provide a detailed analysis as well as a right physical interpretation of the EIS results, the data was fitted with an electric equivalent circuit based on the EIS and the microscopic assessments. The results registered three different time constants (TCs) of the PVA/SA scaffold which indicated different layers at different depths of the scaffold. The TCs changed their dielectric properties depending on the PVA/SA concentration. The 4 wt% system showed the highest biocompatibility properties, given that its resistance and electrochemical capacitance show the formation of a mature-stage cell interaction of HGF. The EIS data offers an exhaustive analysis of the biological activity of the cell response in real time to determine its biocompatibility features. Fluorescence analysis demonstrated a heterogeneous growth of the HGF on the PVA/SA scaffold surface.

    更新日期:2019-09-16
  • Effect of static magnetic field on mold corrosion of printed circuit boards
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-09-13
    Jirui Wang, Ziheng Bai, Kui Xiao, Xueming Li, Qianqian Liu, Xuan Liu, Junsheng Wu, Lin Lu, Chaofang Dong
    更新日期:2019-09-14
  • Rapid recognition and determination of tryptophan by carbon nanotubes and molecularly imprinted polymer-modified glassy carbon electrode
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-09-12
    Yiyong Wu, Peihong Deng, Yaling Tian, Ziyu Ding, Guangli Li, Jun Liu, Zavuga Zuberi, Quanguo He
    更新日期:2019-09-13
  • Acetylcholinesterase modified AuNPs-MoS2-rGO/PI flexible film biosensor: Towards efficient fabrication and application in paraoxon detection
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-09-11
    Lingpu Jia, Yaxin Zhou, Kaipeng Wu, Qingling Feng, Chunming Wang, Ping He
    更新日期:2019-09-12
  • Effect of anaerobic sludge on the bioelectricity generation enhancement of bufferless single-chamber microbial fuel cells
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-09-11
    Ying Lv, Yue Wang, Yueping Ren, Xiufen Li, Xinhua Wang, Jian Li
    更新日期:2019-09-12
  • Gold nanocubes embedded biocompatible hybrid hydrogels for electrochemical detection of H2O2
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-09-07
    Pandiaraj Manickam, Arti Vashist, Sekar Madhu, Mohanraj Sadasivam, Arunkumar Sakthivel, Ajeet Kaushik, Madhavan Nair

    Smart electrochemical biosensors have emerged as a promising alternative analytical diagnostic tool in recent clinical practice. However, improvement in the biocompatibility and electrical conductivity of the biosensor matrix and the immobilization of various bioactive molecules such as enzymes still remain challenging. The present research reports the synthesis of a biocompatible hydrogel network and its integration with gold nanocubes (AuNCs) for developing a novel biosensor with improved functionality. The interpenetrating hydrogel network consist of biopolymers developed using graft co-polymerization of β-cyclodextrin (β-CD) and chitosan (CS). The novelty of this work is in integrating the CS-g-β-CD hydrogel network with conductive AuNCs for improving hydrogel conductivity, biosensor sensitivity and use of the material for a biocompatible sensor. The present protocol advances the state of the art for the utilization of biopolymeric hydrogels system in synergy with an enzymatic biosensing protocol for exclusively detecting hydrogen peroxide (H2O2). Immobilization of the mitochondrial protein, cytochrome c (cyt c) into the hydrogel nanocomposite matrix was performed via thiol cross-linking. This organic-inorganic hybrid nanocomposite hydrogel matrix exhibited high biocompatibility (RAW 264.7 and N2a cell lines), improved electrical conductivity to attain high sensitivity (1.2 mA mM−1 cm−2) and a low detection limit (15 × 10−9 M) for H2O2.

    更新日期:2019-09-07
  • Understanding the role of calcium-mediated cell death in high-frequency irreversible electroporation
    Bioelectrochemistry (IF 4.474) Pub Date : 2019-09-06
    Elisa M. Wasson, Nastaran Alinezhadbalalami, Rebecca M. Brock, Irving C. Allen, Scott S. Verbridge, Rafael V. Davalos

    High-frequency irreversible electroporation (H-FIRE) is an emerging electroporation-based therapy used to ablate cancerous tissue. Treatment consists of delivering short, bipolar pulses (1–10 μs) in a series of 80–100 bursts (1 burst/s, 100 μs on-time). Reducing pulse duration leads to reduced treatment volumes compared to traditional IRE, therefore larger voltages must be applied to generate ablations comparable in size. We show that adjuvant calcium enhances ablation area in vitro for H-FIRE treatments of several pulse durations (1, 2, 5, 10 μs). Furthermore, H-FIRE treatment using 10 μs pulses delivered with 1 mM CaCl2 results in cell death thresholds (771 ± 129 V/cm) comparable to IRE thresholds without calcium (698 ± 103 V/cm). Quantifying the reversible electroporation threshold revealed that CaCl2 enhances the permeabilization of cells compared to a NaCl control. Gene expression analysis determined that CaCl2 upregulates expression of eIFB5 and 60S ribosomal subunit genes while downregulating NOX1/4, leading to increased signaling in pathways that may cause necroptosis. The opposite was found for control treatment without CaCl2 suggesting cells experience an increase in pro survival signaling. Our study is the first to identify key genes and signaling pathways responsible for differences in cell response to H-FIRE treatment with and without calcium.

    更新日期:2019-09-06
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