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Sexually Dimorphic Growth Stimulation in a Strain of Growth Hormone Transgenic Coho Salmon ( Oncorhynchus kisutch ) Mar. Biotechnol. Pub Date : 2021-01-22 Michelle T. T. Chan, Annette Muttray, Dionne Sakhrani, Krista Woodward, Jin-Hyoung Kim, Kris A. Christensen, Ben F. Koop, Robert H. Devlin
Growth hormone (GH) transgenic fish often exhibit remarkable transformations in growth rate and other phenotypes relative to wild-type. The 5750A transgenic coho salmon strain exhibits strong sexually dimorphic growth, with females possessing growth stimulation at a level typical of that seen for both sexes in other strains harbouring the same gene construct (e.g. M77), while males display a modest
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Collagen from Marine Sources and Skin Wound Healing in Animal Experimental Studies: a Systematic Review Mar. Biotechnol. Pub Date : 2021-01-06 Matheus Almeida Cruz, Tiago Akira Araujo, Ingrid Regina Avanzi, Julia Risso Parisi, Ana Laura Martins de Andrade, Ana Claudia Muniz Rennó
Collagen (Col) from marine organisms has been emerging as an important alternative for commercial Col and it has been considered highly attractive by the industry. Despite the positive effects of Col from marine origin, there is still limited understanding of the effects of this natural biomaterial in the process of wound healing in animal studies. In this context, the purpose of this study was to
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Characterization of Macroscopic Colony-Forming Filamentous Cyanobacteria from Okinawan Coasts as Potential Sources of Bioactive Compounds Mar. Biotechnol. Pub Date : 2020-11-26 Handung Nuryadi, Shimpei Sumimoto, Toshiaki Teruya, Kiyotake Suenaga, Shoichiro Suda
Marine macroscopic colony-forming filamentous (MMCFF) cyanobacteria are considered as prolific producers of bioactive compounds. Thus, knowledge of the diversity of MMCFF cyanobacteria as related to bioactive compound production has become very important. However, basic taxonomic studies of MMCFF cyanobacteria are lacking. Many cyanobacterial taxa are still misidentified or undescribed. In this study
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Characterization of Enterobacter cloacae BAGM01 Producing a Thermostable and Alkaline-Tolerant Rhamnolipid Biosurfactant from the Gulf of Mexico Mar. Biotechnol. Pub Date : 2020-11-19 Nidya Fabiola Curiel-Maciel, Fernando Martínez-Morales, Alexei Fedorovish Licea-Navarro, Brandt Bertrand, A. Berenice Aguilar-Guadarrama, Nashbly Sarela Rosas-Galván, Daniel Morales-Guzmán, Nancy Rivera-Gómez, Rosa Maria Gutiérrez-Ríos, María R. Trejo-Hernández
The search for novel biosurfactants (Bs) requires the isolation of microorganisms from different environments. The Gulf of Mexico (GoM) is a geographical area active in the exploration and exploitation of hydrocarbons. Recent metagenomic and microbiologic studies in this area suggested a potential richness for novel Bs microbial producers. In this work, nineteen bacterial consortia from the GoM were
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Genome-Wide Association Study Identifies Genomic Loci of Sex Determination and Gonadosomatic Index Traits in Large Yellow Croaker ( Larimichthys crocea ) Mar. Biotechnol. Pub Date : 2020-11-16 Huanling Lin, Zhixiong Zhou, Ji Zhao, Tao Zhou, Huaqiang Bai, Qiaozhen Ke, Fei Pu, Weiqiang Zheng, Peng Xu
Larimichthys crocea is one of the traditional marine culture fishes in China, widely distributed in South China Sea, East Sea, and southern Yellow Sea. Sex dimorphism is evident in this species that females present a substantial growth strength than males, suggesting breeding females could obtain more economic benefits in L. crocea aquaculture industry. With the continuous expansion of aquaculture
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In Vitro Antitumor Potential of Sulfated Polysaccharides from Seaweed Caulerpa cupressoides var. flabellata Mar. Biotechnol. Pub Date : 2020-11-10 Jefferson da Silva Barbosa, Laís Cristina Gusmão Ferreira Palhares, Cynthia Haynara Ferreira Silva, Diego Araujo Sabry, Suely Ferreira Chavante, Hugo Alexandre Oliveira Rocha
Seaweeds are important source of bioactive compounds, including sulfated polysaccharides (SP). Because of their structural heterogeneity, these compounds are promising sources of anticancer compounds. SP from brown and red seaweeds have shown antimelanoma activity in different in vitro and in vivo models. However, SP from green seaweed are still poorly evaluated. Therefore, SP were extracted from the
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Correction to: Discovery of a Novel Gene Conferring Tellurite Tolerance through Tellurite Reduction to Escherichia coli Transformant in Marine Sediment Metagenomic Library Mar. Biotechnol. Pub Date : 2020-11-09 Madison Pascual Munar, Hirokazu Takahashi, Yoshiko Okamura
A Correction to this paper has been published: https://doi.org/10.1007/s10126-020-10008-1
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Comparative Genomic and Transcriptomic Analyses Revealed Twenty-Six Candidate Genes Involved in the Air-Breathing Development and Function of the Bighead Catfish Clarias macrocephalus Mar. Biotechnol. Pub Date : 2020-10-28 Xiaoli Ma, Baofeng Su, Max Bangs, Veronica Alston, Nathan J. C. Backenstose, Rhoda Mae Simora, Wenwen Wang, De Xing, Shangjia Li, Zhi Ye, Anthony G. Moss, Thuy-Yen Duong, Xu Wang, Rex A. Dunham
The bighead catfish (Clarias macrocephalus) and channel catfish (Ictalurus punctatus) are freshwater species in the Siluriformes order. C. macrocephalus has both gills and modified gill structures serving as an air-breathing organ (ABO), while I. punctatus does not possess such an organ, and cannot breathe in air, providing an excellent model for studying the molecular basis of ABO development in teleost
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Genomic and Transcriptomic Analyses of Bioluminescence Genes in the Enope Squid Watasenia scintillans Mar. Biotechnol. Pub Date : 2020-10-24 Masa-aki Yoshida, Junichi Imoto, Yuri Kawai, Satomi Funahashi, Ryuhei Minei, Yuki Akizuki, Atsushi Ogura, Kazuhiko Nakabayashi, Kei Yura, Kazuho Ikeo
Watasenia scintillans, a sparkling enope squid, has bioluminescence organs to illuminate its body with its own luciferase activity. To clarify the molecular mechanism underlying its scintillation, we analysed high-throughput sequencing data acquired previously and obtained draft genome sequences accomplished with comparative genomic data among the cephalopods. The genome mapped by transcriptome data
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Antifouling Activity of Meroterpenes Isolated from the Ascidian Aplidium aff. densum Mar. Biotechnol. Pub Date : 2020-10-22 Annabel Levert, Valentin Foulon, Marilyne Fauchon, Nathalie Tapissier-Bontemps, Bernard Banaigs, Claire Hellio
The settlement and growth of fouling organisms on man-made surfaces can be prevented by the application of antifouling paints containing active compounds (biocides, heavy metals), most of which are toxic to non-target organisms. As part of our research program in chemical ecology and blue biotechnology, we are conducting studies to investigate the natural defence mechanisms of marine organisms that
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Growth Performance and Transcriptomic Response of Warm-Acclimated Hybrid Abalone Haliotis rufescens (♀) × H. corrugata (♂) Mar. Biotechnol. Pub Date : 2020-10-11 M. A. Tripp-Valdez, F. Cicala, C. E. Galindo-Sánchez, K. D. Chacón-Ponce, E. López-Landavery, F. Díaz, D. Re-Araujo, F. Lafarga-De la Cruz
Along the Pacific coast of the Baja California Peninsula (Mexico), abalone represents one of the most lucrative fisheries. As wild populations are currently depleted, abalone farm production aims to balance the decreasing populations with the increasing demand. The Mexican abalone aquaculture is almost entirely based on red abalone (Haliotis rufescens). However, the increasing frequency of extreme
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A Chromosomal-scale Reference Genome of the Kelp Grouper Epinephelus moara Mar. Biotechnol. Pub Date : 2020-10-08 Qian Zhou, Haoyang Gao, Hao Xu, Haoran Lin, Songlin Chen
The kelp grouper, Epinephelus moara, is a carnivorous coral reef fish widely distributing in the Indo-Pacific Oceans. Compared to other grouper species, E. moara is featured for its wide tolerance to environmental stresses, such as temperature and salinity. In addition, it is popularly used as a breeding species for hybrid grouper production. Here, we report the de novo sequencing and assembly of E
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Correction to: Comparative Gene Analysis Focused on Silica Cell Wall Formation: Identification of Diatom-Specific SET Domain Protein Methyltransferases Mar. Biotechnol. Pub Date : 2020-10-01 Michiko Nemoto, Sayako Iwaki, Hisao Moriya, Yuki Monden, Takashi Tamura, Kenji Inagaki, Shigeki Mayama, Kiori Obuse
[Original sentence on Page 560, Line 34–37] The present BacSET protein family might be a novel, diatom-specific family of methyltransferases that target unique substrates, such as cell wall formation-related proteins and LCPAs.
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Transcriptome Profiling Reveals the Sexual Dimorphism of Gene Expression Patterns during Gonad Differentiation in the Half-Smooth Tongue Sole ( Cynoglossus semilaevis ) Mar. Biotechnol. Pub Date : 2020-09-29 Genmei Lin, Dong Gao, Jianguo Lu, Xiaowen Sun
The half-smooth tongue sole (Cynoglossus semilaevis), one of the most economically-important fish species in China, exhibits sexually dimorphic growth. An understanding of sex-related gene expression patterns in the tongue sole may inform sex regulation and breeding processes that increase fish production. However, the gene expression patterns during gonad development in the tongue sole remain unknown
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The Function of β-1,4-Glucuronosyltransferase WelK in the Sphingan WL Gum Biosynthesis Process in Marine Sphingomonas sp. WG. Mar. Biotechnol. Pub Date : 2020-09-26 Hui Li,Kehui Li,Zhongrui Guo,Han Xue,Jing Li,Sixue Ji,Jiqian Wang,Hu Zhu
The marine-derived polysaccharide WL gum produced by Sphingomonas sp. WG showed commercial utility potential in ink, food, and oil industries. A β-1,4-glucuronosyltransferase WelK was predicted to catalyze the transfer of glucuronic acid from UDP-glucuronic acid to glucosyl-α-pyrophosphorylpolyprenol intermediate in the WL gum biosynthesis process. Its function was evaluated by bioinformatical analysis
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A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria. Mar. Biotechnol. Pub Date : 2020-09-26 Chika Shono,Dwi Ariyanti,Koichi Abe,Yuta Sakai,Ippei Sakamoto,Kaori Tsukakoshi,Koji Sode,Kazunori Ikebukuro
In this study, we developed a green light-regulated T7 RNA polymerase expression system (T7 RNAP system), to provide a novel and versatile high-expression system for cyanobacteria without using any chemical inducer, realizing high expression levels comparable with previously reported for recombinant gene expression in cyanobacteria. The T7 RNAP system was constructed and introduced into Synechocystis
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Efficient Genome Editing in Multiple Salmonid Cell Lines Using Ribonucleoprotein Complexes. Mar. Biotechnol. Pub Date : 2020-09-18 Remi L Gratacap,Ye Hwa Jin,Marina Mantsopoulou,Ross D Houston
Infectious and parasitic diseases have major negative economic and animal welfare impacts on aquaculture of salmonid species. Improved knowledge of the functional basis of host response and genetic resistance to these diseases is key to developing preventative and treatment options. Cell lines provide valuable models to study infectious diseases in salmonids, and genome editing using CRISPR/Cas systems
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Elucidation of Gut Microbiota in Mud Crab Scylla paramamosain Challenged to WSSV and Aeromonas hydrophila. Mar. Biotechnol. Pub Date : 2020-09-10 Shanmeng Lin,Tongtong Kong,Xin Ren,Shengkang Li,Yi Gong
Mud crab Scylla paramamosain (S. paramamosain) is an economically important marine crab species around the world. White spot syndrome virus (WSSV) and Aeromonas hydrophila (AH) are pathogens during mud crab mariculture. It has been reported that gut microbiota possessed a great impact on the host development, nutrition, immunity, and disease resistance. However, little information was known about the
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Transcriptome Analysis of the Liver and Muscle Tissues of Black Carp (Mylopharyngodon piceus) of Different Growth Rates. Mar. Biotechnol. Pub Date : 2020-09-10 Jiahua Zhang,Yubang Shen,Xiaoyan Xu,Yafan Dai,Jiale Li
In this study, we used RNA-seq to analyze the muscle and liver tissues of black carps (Mylopharyngodon piceus) of different growth rates from the same batch to evaluate their growth traits. We have two groups; they are the black carp group with fast-growth rate and the slow-growth rate. A total of 23,132 genes were enriched in the Gene Ontology analysis, and 285 related pathways were found in the Kyoto
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Integrated mRNA and Small RNA Sequencing Reveals Regulatory Expression of Larval Metamorphosis of the Razor Clam. Mar. Biotechnol. Pub Date : 2020-09-04 Donghong Niu,Beibei Li,Shumei Xie,Zhiguo Dong,Jiale Li
The razor clam, Sinonovacula constricta, is an important economic marine shellfish, and its larval development involves obvious morphological and physiological changes. MicroRNA plays a key role in the physiological changes of the organism through regulating targeted mRNA. This study performed miRNA–mRNA sequencing for eight different developmental stages of S. constricta using Illumina sequencing
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Elovl2 But Not Elovl5 Is Essential for the Biosynthesis of Docosahexaenoic Acid (DHA) in Zebrafish: Insight from a Comparative Gene Knockout Study. Mar. Biotechnol. Pub Date : 2020-09-03 Chengjie Liu,Ding Ye,Houpeng Wang,Mudan He,Yonghua Sun
Teleost fish can synthesize one of the major omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFAs), docosahexaenoic acid (DHA, 22:6n-3), from dietary α-linolenic acid (ALA; 18:3n-3), via elongase of very long–chain fatty acid (Elovl) and fatty acid desaturase (Fads). However, it remains unclear which elongase is primarily responsible for the endogenous synthesis of DHA. Here, in this study
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Diel Variation in CC Chemokine Gene Expression in the Japanese Pufferfish Takifugu rubripes. Mar. Biotechnol. Pub Date : 2020-09-02 Yuri Tsutsui,Teika Onoue,Jun-Ichi Hikima,Masahiro Sakai,Tomoya Kono
CC chemokines are key molecules in the regulation of leukocyte trafficking to the site of injury, infection, or inflammation. In recent years, some mammalian chemokines have been shown to exhibit rhythmic expression, regulated by clock genes. However, the rhythmic expression of chemokines in teleost fish remains unknown. In the present study, the diel variation of teleost CC chemokine genes was investigated
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Non-coding RNA Expression Patterns of Two Different Teleost Gonad Maturation Stages. Mar. Biotechnol. Pub Date : 2020-09-02 Maria Papadaki,Elisavet Kaitetzidou,Constantinos C Mylonas,Elena Sarropoulou
Non-coding RNAs (ncRNAs) are involved in several different regulatory pathways including reproduction. In teleost fish, efficacious reproduction is heavily dependent on the completion of the reproductive cycle. The presence of ncRNA, however, and their expression dynamics and putative regulatory role in mature and immature gonads have not yet been extensively explored. Therefore, the abundance of ncRNAs
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Comparison of Areal Productivity of Nannochloropsis oceanica Between Lab-Scale and Industrial-Scale Raceway Pond. Mar. Biotechnol. Pub Date : 2020-08-28 Takeshi Saito,Takahiro Ichihara,Hidetoshi Inoue,Takafumi Uematsu,Saki Hamada,Takaaki Watanabe,Yasushi Takimura,Jason Webb
The areal biomass productivities (g−1 m−2 day−1) of Nannochloropsis oceanica between different sizes of way ponds were compared. Sequential batch cultivation using 2-m2, 20-m2, and 200-m2 raceway ponds with an industrial scale 4000-m2 raceway as the main culture was conducted in summer and autumn during 2017 at Whyalla, Australia. Areal productivities of sequential batch cultivation during the same
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MiRNAs-Modulation of Nrf2 Signaling Networks in Regulation Oxidative Stress of Chinese Perch Skeletal Muscle After Fasting Treatment. Mar. Biotechnol. Pub Date : 2020-08-25 Ping Wu,Lin Chen,Jia Cheng,Yaxiong Pan,Xinhong Guo,Wuying Chu,Jianshe Zhang,Xuanming Liu
Nrf2 is an important transcription factor involved in the antioxidant response and is widely expressed in animal tissues. The function of Nrf2 is regulated by its negative regulator Keap1 by inducing its cytoplasmic degradation. Recent studies have suggested that Nrf2 is also regulated post-transcriptionally via miRNAs. However, to date, how miRNAs regulate Nrf2 in fish skeletal muscles is unknown
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Biotransformation of Ethinylestradiol by Whole Cells of Brazilian Marine-Derived Fungus Penicillium oxalicum CBMAI 1996. Mar. Biotechnol. Pub Date : 2020-08-24 Thayane Melo de Queiroz,Javier Ellena,André L M Porto
In this study, we report our contribution to the application of whole cells of Brazilian marine-derived fungi in the biotransformation of ethinylestradiol 1. A preliminary screening with twelve marine-derived fungi strains revealed that the fungus Penicillium oxalicum CBMAI 1996 promoted the biotransformation of ethinylestradiol 1. Then, P. oxalicum CBMAI 1996 was employed in the reactions in decaplicate
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Genome Mining Reveals the Biosynthetic Pathways of Polyhydroxyalkanoate and Ectoines of the Halophilic Strain Salinivibrio proteolyticus M318 Isolated from Fermented Shrimp Paste. Mar. Biotechnol. Pub Date : 2020-08-22 Doan Van Thuoc,Tran Thi Loan,Trieu Anh Trung,Nguyen Van Quyen,Quach Ngoc Tung,Phi Quyet Tien,Kumar Sudesh
Salinivibrio proteolyticus M318, a halophilic bacterium isolated from fermented shrimp paste, is able to produce polyhydroxyalkanoate (PHA) from different carbon sources. In this study, we report the whole-genome sequence of strain M138, which comprises 2 separated chromosomes and 2 plasmids, and the complete genome contains 3,605,935 bp with an average GC content of 49.9%. The genome of strain M318
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Transgenic Expression and Genome Editing by Electroporation of Zebrafish Embryos. Mar. Biotechnol. Pub Date : 2020-08-04 Changqing Zhang,Ziheng Ren,Zhiyuan Gong
Microinjection is predominantly used to produce genetically modified fish. However, there are certain difficulties involved in some fish species. In this study, we tested an alternative method to produce genetically modified zebrafish by delivering DNA and other materials into embryos by electroporation. We optimized the electroporation conditions of a square wave electroporation system that work efficiently
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Transcriptome Analyses of Immune System Behaviors in Primary Polyp of Coral Acropora digitifera Exposed to the Bacterial Pathogen Vibrio coralliilyticus under Thermal Loading. Mar. Biotechnol. Pub Date : 2020-07-21 Toshiyuki Takagi,Yuki Yoshioka,Yuna Zayasu,Noriyuki Satoh,Chuya Shinzato
Elevated sea surface temperature associated with global warming is a serious threat to coral reefs. Elevated temperatures directly or indirectly alter the distribution of coral-pathogen interactions and thereby exacerbate infectious coral diseases. The pathogenic bacterium Vibrio coralliilyticus is well-known as a causative agent of infectious coral disease. Rising sea surface temperature promotes
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Genome-Wide Association Study of Body Shape-Related Traits in Large Yellow Croaker (Larimichthys crocea). Mar. Biotechnol. Pub Date : 2020-07-15 Shengnan Kong,Zhixiong Zhou,Tao Zhou,Ji Zhao,Lin Chen,Huanling Lin,Fei Pu,Qiaozhen Ke,Huaqiang Bai,Peng Xu
Large yellow croaker (Larimichthys crocea) is one of the most important cultured marine fish on the southeast coast of China. Its body shape is important for the aquaculture industry since it affects the behavior such as swimming, ingesting, and evading, as well as customer preference. Due to the greater consumer demand of small head, slender body large yellow croaker, selecting and breeding of slender
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miR-9 and miR-263 Regulate the Key Genes of the ERK Pathway in the Ovary of Mud Crab Scylla paramamosain. Mar. Biotechnol. Pub Date : 2020-07-11 Mingcan Zhou,Xiwei Jia,Haifu Wan,Shuhong Wang,Xin Zhang,Ziping Zhang,Yilei Wang
Mud crab Scylla paramamosain is one of the most important economic crabs in China. The molecular regulatory mechanism of ovarian development has received considerable attention in recent years. Some studies found that ERK (extracellular signal-regulated protein kinase) signaling pathway plays an important role in ovarian development and is negatively regulated by microRNAs (miRNAs). However, the study
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Red and White Chinook Salmon (Oncorhynchus tshawytscha): Differences in the Transcriptome Profile of Muscle, Liver, and Pylorus. Mar. Biotechnol. Pub Date : 2020-06-26 Angelico Madaro,Ole Torrissen,Paul Whatmore,Santosh P Lall,Jerome Schmeisser,Viviane Verlhac Trichet,Rolf Erik Olsen
Astaxanthin (Ax), the main carotenoid responsible for the distinct red flesh color in salmonids (Oncorhynchus, Salvelinus, Salmo, and Parahucho), is added to the diet of farmed fish at a substantial cost. Despite the great economical value for the salmon industry, the key molecular mechanisms involved in the regulation of muscle coloration are poorly understood. Chinook salmon (Oncorhynchus tshawytscha)
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The Polymorphism of LvMMD2 and Its Association with Growth Traits in Litopenaeus vannamei. Mar. Biotechnol. Pub Date : 2020-06-23 Quanchao Wang,Yang Yu,Qian Zhang,Zheng Luo,Xiaojun Zhang,Jianhai Xiang,Fuhua Li
The Pacific white shrimp Litopenaeus vannamei is one of the major economic aquaculture species. The growth trait is considered as the most important trait in L. vannamei aquaculture. Identification of the genetic components underlying growth-related traits in L. vannamei could be useful for the selective breeding of growth trait. Our previous work identified several growth-related SNPs by genome-wide
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Chloroplast Genetic Engineering of a Unicellular Green Alga Haematococcus pluvialis with Expression of an Antimicrobial Peptide. Mar. Biotechnol. Pub Date : 2020-06-13 Kang Wang,Yulin Cui,Yinchu Wang,Zhengquan Gao,Tianzhong Liu,Chunxiao Meng,Song Qin
The purpose of this study was to express an antimicrobial peptide in the chloroplast to further develop the plastid engineering of H. pluvialis. Homologous targeting of the 16S-trnI/trnA-23S region and four endogenous regulatory elements, including the psbA promoter, rbcL promoter, rbcL terminator, and psbA terminator in H. pluvialis, were performed to construct a chloroplast transformation vector
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Identification of Functional SSR Markers in Freshwater Ornamental Shrimps Neocaridina denticulata Using Transcriptome Sequencing. Mar. Biotechnol. Pub Date : 2020-06-11 Chang-Wen Huang,Pei-Yun Chu,Yu-Fang Wu,Wei-Ren Chan,Yeh-Hao Wang
The amazing colors and patterns are fascinating characteristics in all of the aquarium species. However, genetic and breeding molecular investigations of ornamental shrimps are rather limited. Here, we present the first transcriptomic analysis and application of microsatellites based on the chromatophore-encoded genes of Neocaridina denticulata to assist freshwater ornamental shrimp germplasm enhancement
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Diet-Induced Physiological Responses in the Liver of Atlantic Salmon (Salmo salar) Inferred Using Multiplex PCR Platforms. Mar. Biotechnol. Pub Date : 2020-06-04 Albert Caballero-Solares,Xi Xue,Beth M Cleveland,Maryam Beheshti Foroutani,Christopher C Parrish,Richard G Taylor,Matthew L Rise
The simultaneous quantification of several transcripts via multiplex PCR can accelerate research in fish physiological responses to diet and enable the development of superior aquafeeds for farmed fish. We designed two multiplex PCR panels that included assays for 40 biomarker genes representing key aspects of fish physiology (growth, metabolism, oxidative stress, and inflammation) and 3 normalizer
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Genetic and Morphological Characteristics in the Local Population of the Landlocked Salmon Oncorhynchus masou Originally Distributed in Kanagawa Prefecture, Japan. Mar. Biotechnol. Pub Date : 2020-06-03 Taiki Okabe,Naoyuki Suguro,Tomoko Koito,Kento Endo,Haruo Sugita,Shiro Itoi
Oncorhynchus masou, including subspecies of Oncorhynchus masou masou (yamame) and Oncorhynchus masou ishikawae (amago), is one of the salmonid groups impacted by human activity such as dam construction and release of non-native salmonids. In this study, we investigated the genetic structure of O. masou populations in the Sakawa and Sagami Rivers, Japan, by sequencing the mitochondrial control region
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Comparative Gene Analysis Focused on Silica Cell Wall Formation: Identification of Diatom-Specific SET Domain Protein Methyltransferases. Mar. Biotechnol. Pub Date : 2020-06-03 Michiko Nemoto,Sayako Iwaki,Hisao Moriya,Yuki Monden,Takashi Tamura,Kenji Inagaki,Shigeki Mayama,Kiori Obuse
Silica cell walls of diatoms have attracted attention as a source of nanostructured functional materials and have immense potential for a variety of applications. Previous studies of silica cell wall formation have identified numerous involved proteins, but most of these proteins are species-specific and are not conserved among diatoms. However, because the basic process of diatom cell wall formation
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Transcriptomic Response to Selective Breeding for Fast Growth in Rainbow Trout (Oncorhynchus mykiss). Mar. Biotechnol. Pub Date : 2020-05-26 Beth M Cleveland,Guangtu Gao,Timothy D Leeds
Genetic improvement for faster growth is a conventional approach to increase growth rates in aquaculture species; however, the genetic and physiological factors regulating growth performance in fish are not fully characterized. The objective of this study was to identify physiological mechanisms associated with faster growth rates by comparing the liver and muscle transcriptome of a rainbow trout line
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Molecular Identification, Characterization, and Expression Analysis of a Metallothionein Gene from Septifer virgatus. Mar. Biotechnol. Pub Date : 2020-05-21 Akihiro Sakatoku,Masahito Ishikawa,Kanna Yamazaki,Tomoya Nakamachi,Hiroyuki Kamachi,Daisuke Tanaka,Shogo Nakamura
This study provides a preliminary characterization of a metallothionein (MT) gene in Septifer virgatus and highlights its potential use in biomonitoring. The full-length SvMT cDNA and the complete sequence of the SvMT gene were identified using reverse transcriptase PCR coupled with the rapid amplification of cDNA ends and the primer walking method. The SvMT cDNA encodes a protein of 72 amino acids
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Isolation of a Male-Specific Molecular Marker and Development of a Genetic Sex Identification Technique in Spotted Knifejaw (Oplegnathus punctatus). Mar. Biotechnol. Pub Date : 2020-05-19 Ming Li,Hao Xu,Wenteng Xu,Qian Zhou,Xiwen Xu,Ying Zhu,Weiwei Zheng,Wensheng Li,Zunfang Pang,Songlin Chen
Sex-specific DNA markers are very helpful for identifying genetic sex and studying sex determination mechanisms in fish. To identify the sex-specific markers of spotted knifejaw (Oplegnathus punctatus), we performed a comparative analysis of the female and male genomes. In this study, an 18 bp insertion was identified in the male genome after verification by sequencing depth and PCR. An effective and
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First High-Density Linkage Map and QTL Fine Mapping for Growth-Related Traits of Spotted Sea bass (Lateolabrax maculatus). Mar. Biotechnol. Pub Date : 2020-05-19 Yang Liu,Haolong Wang,Haishen Wen,Yue Shi,Meizhao Zhang,Xin Qi,Kaiqiang Zhang,Qingli Gong,Jifang Li,Feng He,Yanbo Hu,Yun Li
Possessing powerful adaptive capacity and a pleasant taste, spotted sea bass (Lateolabrax maculatus) has a broad natural distribution and is one of the most popular mariculture fish in China. However, the genetic improvement program for this fish is still in its infancy. Growth is the most economically important trait and is controlled by quantitative trait loci (QTL); thus, the identification of QTLs
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The miR-15/16 Cluster Is Involved in the Regulation of Vertebrate LC-PUFA Biosynthesis by Targeting pparγ as Demonostrated in Rabbitfish Siganus canaliculatus. Mar. Biotechnol. Pub Date : 2020-05-16 Junjun Sun,Cuiying Chen,Cuihong You,Yang Liu,Hongyu Ma,Óscar Monroig,Douglas R Tocher,Shuqi Wang,Yuanyou Li
Post-transcriptional regulatory mechanisms play important roles in the regulation of long-chain (≥ C20) polyunsaturated fatty acid (LC-PUFA) biosynthesis. Here, we address a potentially important role of the miR-15/16 cluster in the regulation of LC-PUFA biosynthesis in rabbitfish Siganus canaliculatus. In rabbitfish, miR-15 and miR-16 were both highly responsive to fatty acids affecting LC-PUFA biosynthesis
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Taxonomic Distribution of Tetrodotoxin in Acotylean Flatworms (Polycladida: Platyhelminthes). Mar. Biotechnol. Pub Date : 2020-05-15 Maho Kashitani,Taiki Okabe,Hikaru Oyama,Kaede Noguchi,Haruka Yamazaki,Rei Suo,Tetsushi Mori,Haruo Sugita,Shiro Itoi
Tetrodotoxin (TTX), also known as pufferfish toxin, causes a respiratory disorder by blocking neurotransmission, with voltage-gated sodium channel inhibition on muscle and nerve tissues. The toxin is widely distributed across vertebrates, invertebrates and bacteria. Therefore, it is generally thought that TTX in pufferfish accumulates via the food webs, beginning with marine bacteria as a primary producer
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A Facile and Sensitive DNA Sensing of Harmful Algal Blooms Based on Graphene Oxide Nanosheets. Mar. Biotechnol. Pub Date : 2020-05-02 Le Qiang,Yu Zhang,Chao Wu,Yingkuan Han,Suchun Wang,Yanyan Wang,Congcong Zhang,Guangzhou Liu,Qi Wu,Hong Liu,Ian R Jenkinson,Jun Sun,Lin Han
Gene detection has important applications in biology, biomedical engineering, clinical, environmental, and marine fields. Rapid, sensitive, and selective recognition of specific genes is essential in practical applications. In this study, we describe a facile and sensitive DNA sensing platform for specific and quantitative detection of Heterosigma akashiwo, which is one of the causative agents of red
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siRNA-Mediated MrIAG Silencing Induces Sex Reversal in Macrobrachium rosenbergii. Mar. Biotechnol. Pub Date : 2020-04-27 Kianann Tan,Miao Zhou,Huigong Jiang,Donghuo Jiang,Yanhe Li,Weimin Wang
The insulin-like androgenic gland (IAG) gene is well known in male crustacean, and it is a key regulator in male sexual differentiation and maintaining the male sexual characteristic. The neo-female can be produced by silencing the MrIAG (Macrobrachium rosenbergii Insulin-like Androgenic Gland) in male Macrobrachium rosenbergii. This is the first time to use siRNA approach to silenced MrIAG in male
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Evaluation of the In Vivo Biological Effects of Marine Collagen and Hydroxyapatite Composite in a Tibial Bone Defect Model in Rats. Mar. Biotechnol. Pub Date : 2020-04-25 Julia Risso Parisi,Kelly Rossetti Fernandes,Matheus de Almeida Cruz,Ingrid Regina Avanzi,Alan de França Santana,Giovanna Caroline Aparecida do Vale,Ana Laura Martins de Andrade,Cíntia Pereira de Góes,Carlos Alberto Fortulan,Eliandra de Sousa Trichês,Renata Neves Granito,Ana Claudia Muniz Rennó
One of the most promising strategies to improve the biological performance of bone grafts is the combination of different biomaterials. In this context, the aim of this study was to evaluate the effects of the incorporation of marine spongin (SPG) into Hydroxyapatite (HA) for bone tissue engineering proposals. The hypothesis of the current study is that SPG into HA would improve the biocompatibility
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Identification of the Domains Involved in Promotion of Silica Formation in Glassin, a Protein Occluded in Hexactinellid Sponge Biosilica, for Development of a Tag for Purification and Immobilization of Recombinant Proteins Mar. Biotechnol. Pub Date : 2020-04-14 Michika Nishi, Hiroki Kobayashi, Taro Amano, Yuto Sakate, Tomohiro Bito, Jiro Arima, Katsuhiko Shimizu
Glassin, a protein occluded in biosilica of the hexactinellid sponge Euplectela, promotes silica formation from silicic acid at room temperature and neutral pH and is characterized by its primary structure which consists of a tandem repeat carrying three distinct domains, histidine and aspartic acid-rich (HD) domain, proline-rich (P) domain, and histidine and threonine-rich (HT) domain. The present
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Correction to: Transcriptomic Profiling Provides Insights into Inbreeding Depression in Yesso Scallop Patinopecten yessoensis. Mar. Biotechnol. Pub Date : 2020-01-23 Liang Zhao,Yangping Li,Jiarun Lou,Zhihui Yang,Huan Liao,Qiang Fu,Zhenyi Guo,Shanshan Lian,Xiaoli Hu,Zhenmin Bao
The original version of this article unfortunately contained a mistake in the authorgroup section. Author Zhenmin Bao's given name was incorrectly spelled as "Zhemin Bao".
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Responses of Intertidal Bacterial Biofilm Communities to Increasing p CO 2 Mar. Biotechnol. Pub Date : 2020-03-17 Dorsaf Kerfahi, Ben P. Harvey, Sylvain Agostini, Koetsu Kon, Ruiping Huang, Jonathan M. Adams, Jason M. Hall-Spencer
The effects of ocean acidification on ecosystems remain poorly understood, because it is difficult to simulate the effects of elevated CO2 on entire marine communities. Natural systems enriched in CO2 are being used to help understand the long-term effects of ocean acidification in situ. Here, we compared biofilm bacterial communities on intertidal cobbles/boulders and bedrock along a seawater CO2
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Bisexual Fertile Triploid Zebrafish ( Danio rerio ): a Rare Case Mar. Biotechnol. Pub Date : 2020-04-20 Liangyue Peng; Wen Fu; Xianlong Wu; Sheng He; Han Zhao; Jinhui Liu; Wenbin Liu; Yamei Xiao
Previous studies have suggested that artificially induced triploid zebrafish are exclusively male-biased. Owing to greatly inhibited gonadal development for the artificially induced triploid fish, they are regarded to be sterile in general. In this article, partially fertile bisexual triploid zebrafish are produced by suppressing extrusion of the second polar body by heat shock. Histological observation
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Sex-Specific Transcriptome Sequencing of Zoea I Larvae and Identification of Sex-Linked Genes Using Bulked Segregant Analysis in Pacific White Shrimp Litopenaeus vannamei. Mar. Biotechnol. Pub Date : 2020-04-12 Yue Wang,Yang Yu,Shihao Li,Xiaojun Zhang,Jianhai Xiang,Fuhua Li
In order to learn the sex determination and differentiation process in shrimp, we performed sex-specific transcriptome sequencing of the Zoea I larvae in Litopenaeus vannamei (L. vannamei) using a DNA/RNA co-extraction method. The sex-specific genes and the sex-linked SNPs were identified. De novo assembly of all the clean reads generated 41,270 unigenes with an average length of 1026 bp and an N50
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Rapid Genomic and Epigenetic Alterations in Gynogenetic Carassius auratus Red Var. Derived from Distant Hybridization. Mar. Biotechnol. Pub Date : 2020-04-05 Qinbo Qin,Chongqing Wang,Yuwei Zhou,Huan Qin,Chun Zhao,Li Yang,Tingting Yu,Shaojun Liu
Gynogenesis is an important reproductive mode in fish and is used fairly widely in genetic breeding. Gynogenetic offspring (2n = 100, abbreviated as GRCC) were generated through the distant hybridization of Carassius auratus red var. (2n = 100, RCC) (♀) × Megalobrama amblycephala (2n = 48, BSB) (♂), in which male and female individual both had normal gonadal development. To better understand genomic
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Assessing the Effect of Contaminated and Restored Marine Sediments in Different Experimental Mesocosms Using an Integrated Approach and Mytilus galloprovincialis as a Model. Mar. Biotechnol. Pub Date : 2020-04-02 Stefania Ancora,Federica Rossi,Marina Borgese,Cristina Pirrone,Ilaria Caliani,Simone Cappello,Giuseppe Mancini,Nicola Bianchi,Claudio Leonzio,Giovanni Bernardini,Rosalba Gornati
The research presented here was conducted to ascertain the effectiveness of recovery technologies in remediating a compromised marine environment. The multidisciplinary approach aims to integrate traditional chemical-physical analysis and to assess the biological parameters of Mytilus galloprovincialis within different experimental mesocosms (W, G, and B). In particular, this system was designed to
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In Silico Analysis of ACE Inhibitory Peptides from Chloroplast Proteins of Red Alga Grateloupia asiatica. Mar. Biotechnol. Pub Date : 2020-03-24 Kana Sumikawa,Kentaro Takei,Yuya Kumagai,Takeshi Shimizu,Hajime Yasui,Hideki Kishimura
Inhibition of angiotensin I–converting enzyme (ACE) is one of the key factors to repress high blood pressure. Although many studies have been reported that seaweed protein hydrolysates showed the ACE inhibitory activity, the comprehensive understanding of the relationship was still unclear. In this study, we employed chloroplast genome for in silico analysis and compared it with in vitro experiments
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A Mesohaline Thraustochytrid Produces Extremely Halophilic Alpha-Amylases. Mar. Biotechnol. Pub Date : 2020-03-14 Priyanka V Shirodkar,Usha Devi Muraleedharan,Samir Damare,Seshagiri Raghukumar
Halophilic bacteria are well known to produce highly salt-tolerant enzymes that have unusual applications in biotechnology. Production of halophilic proteins is generally not expected in mesohaline microorganisms. Ulkenia sp. AH-2, a mesohaline, marine straminipilan thraustochytrid isolated from waters of a mangrove ecosystem, produces halophilic alpha-amylases. Four enzyme fractions, viz.., A, B,
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Genome-wide DNA Methylation Analysis of Mantle Edge and Mantle Central from Pearl Oyster Pinctada fucata martensii. Mar. Biotechnol. Pub Date : 2020-03-06 Jiabin Zhang,Shaojie Luo,Zefeng Gu,Yuewen Deng,Yu Jiao
DNA methylation is a type of epigenetic modification that alters gene expression without changing the DNA sequence and mediates some cases of phenotypic plasticity. In this study, we identified six DNA methyltransferase (DNMT) genes and two methyl-CpG binding domain protein2 (MBD2) gene from Pinctada fucata martensii. We also analyzed the genome-wide DNA methylation levels of mantle edge (ME) and mantle
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Synthesis of Knoevenagel Adducts Under Microwave Irradiation and Biocatalytic Ene-Reduction by the Marine-Derived Fungus Cladosporium sp. CBMAI 1237 for the Production of 2-Cyano-3-Phenylpropanamide Derivatives. Mar. Biotechnol. Pub Date : 2020-03-03 Willian Garcia Birolli,Lucas Lima Zanin,David Esteban Quintero Jimenez,André Luiz Meleiro Porto
The organic synthesis has been driven by the need of sustainable processes, which also requires efficiency and cost-effectiveness. In this work, we described the synthesis of nine Knoevenagel adducts between cyanoacetamide and aromatic aldehydes ((E)-2-cyano-3-(phenyl)acrylamide derivatives), employing triethylamine as catalyst under microwave irradiation in 30 min with excellent yields (93–99% yield)
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Transcriptome Profiling and Analysis of Genes Associated with High Temperature-Induced Masculinization in Sex-Undifferentiated Nile Tilapia Gonad. Mar. Biotechnol. Pub Date : 2020-02-22 Jian Teng,Yan Zhao,Hong Ju Chen,Hui Wang,Xiang Shan Ji
Artificially high temperatures during critical thermosensitive periods (TSPs) can induce the sex reversal of Nile tilapia (Oreochromis niloticus) females into pseudomales; Nile tilapia is a GSD + TE (genotypic plus temperature effects) fish species. Previous studies have shown that water temperature affects the expression levels of many genes in the gonad or brain in various teleost species. However
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Cells Isolated from Regenerating Caudal Fin of Sparus aurata Can Differentiate into Distinct Bone Cell Lineages. Mar. Biotechnol. Pub Date : 2020-02-20 Parameswaran Vijayakumar,João Cardeira,Vincent Laizé,Paulo J Gavaia,M Leonor Cancela
Teleosts have the ability to regenerate their caudal fin upon amputation. A highly proliferative mass of undifferentiated cells called blastema forms beneath wound epidermis and differentiates to regenerate all missing parts of the fin. To date, the origin and fate of the blastema is not completely understood. However, current hypotheses suggest that the blastema is comprised of lineage-restricted
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