-
miR-4319 inhibited retinoblastoma cells proliferation, migration, invasion and EMT progress via suppressing CD147 mediated MMPs expression J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-20 Zhiqing Wu, Ling Chen, Chengyue Zhang, Yanfang Liu
Tumor migration is the critical step that lead to the migration in retinoblastoma (RB), in which microRNAs (miRNAs) play important roles. This study aimed to investigate the role of microRNA-4319 (miR-4319) in the development of retinoblastoma by identifying its targets, as well as its underlying regulatory mechanisms. Our data shown that miR-4319 was downregulated in RB tissues and RB cell lines.
-
Dietary modulations of folic acid affect the development of diethylnitrosamine induced hepatocellular carcinoma in a rat model J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-13 Renuka Sharma, Taqveema Ali, Ishwar Negi, Ashim Das, Ajay Duseja, Jyotdeep Kaur
The present study evaluated the role of dietary folate modulations in the development of hepatocellular carcinoma (HCC) in a rat model. Male Wistar rats were given diethylnitrosamine (DEN) carcinogen for a period of 18 weeks in addition to different folate modulations. Biochemical parameters were assayed and liver tissues were examined using various histopathological stains viz. Hematoxylin and eosin
-
Implications of the specific localization of YAP signaling on the epithelial patterning of circumvallate papilla J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-08 Ji-Youn Kim, Tae-Young Kim, Eui-Seon Lee, Yam Prasad Aryal, Elina Pokharel, Shijin Sung, Wern-Joo Sohn, Jae-Young Kim, Jae-Kwang Jung
Circumvallate papilla (CVP) is a distinctively structured with dome-shaped apex, and the surrounding trench which contains over two hundred taste buds on the lateral walls. Although CVP was extensively studied to determine the regulatory mechanisms during organogenesis, it still remains to be elucidated the principle mechanisms of signaling regulations on morphogenesis including taste buds formation
-
Comparison of quantum dot immunofluorescence histochemistry with conventional immunohistochemistry in detecting Helicobacter pylori infection in paraffin-embedded tissues of gastric biopsy J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-08 Weisong Wan, Qinxue Pu, Xin Huang, Daizhen Luo, Yuchang Hu, Yufei Liu
Quantum dots (QDs) are a new type of fluorescent label, which has been widely used in many biological and biomedical imaging applications. In this study, we used QDs-based immunofluorescence histochemistry (QDs-IHC) and conventional immunohistochemistry (IHC) techniques to perform a retrospective analysis on paraffin-embedded tissues of gastric biopsies in 203 patients (112 of which were HP positive
-
Immunoexpression pattern of autophagy mediators in alveolar bone osteoclasts following estrogen withdrawal in female rats J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-06 Rinaldo Florencio-Silva, Gisela Rodrigues da Silva Sasso, Estela Sasso-Cerri, Manuel de Jesus Simões, Paulo Sérgio Cerri
It is known that estrogen deficiency increases osteoclast formation and activity. Autophagy, a cell survival pathway, has been shown to be crucial for osteoclast function. However, little is known about the effects of estrogen depletion on osteoclast autophagy. Here, we evaluated the effects of estrogen deficiency in the immunoexpression of autophagy mediators in alveolar bone osteoclasts of ovariectomized
-
Circular RNA RBM33 contributes to cervical cancer progression via modulation of the miR-758-3p/PUM2 axis J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-05 Yi Ding, Xia Yuan, Wenwen Gu
Cervical cancer (CC) is a gynecological malignant tumor. Circular RNA (hsa_circ_0001772) (circRBM33) is implicated in the tumorigenesis of cancers. Nevertheless, the role of circRBM33 in CC is indistinct. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to evaluate the levels of circRBM33, miR-758-3p, and pumilio RNA binding family member 2 (PUM2) mRNA in tissue samples and cells
-
Expression of NR5A2, NUP153, HNF4A, USP15 and FNDC3B is consistent with their use as novel biomarkers for bovine mammary stem/progenitor cells J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-05 Ratan K. Choudhary, Anthony V. Capuco
Mammary stem cells (MaSC) are essential for growth and maintenance of mammary epithelium. Previous studies have utilized morphological characteristics or retention of bromodeoxyuridine (BrdU) label to identify MaSC and progenitor cells, these approaches may not be feasible or may not identify all resident stem cells. Alternatively, these special cells may be identified by assessing protein and mRNA
-
LncRNA KCNQ1OT1 acts as miR-216b-5p sponge to promote colorectal cancer progression via up-regulating ZNF146 J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-04 Shuang Zhu, Chih-Yen Chen, Yangyang Hao
Long non-coding RNAs (lncRNAs) have shown to act as important regulators in cancer biology. The aim of this study was to investigate the role and mechanism of lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in colorectal cancer (CRC) progression. The abundance of KCNQ1OT1, microRNA-216b-5p (miR-216b-5p) and zinc finger protein 146 (ZNF146) messenger RNA (mRNA) was measured by quantitative
-
Ubiquitin-specific protease 14 (USP14) promotes proliferation and metastasis in pancreatic ductal adenocarcinoma J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-04 Cheng Hang, Chen Gong, Yanfei Fang, Long Chen, Jianxin Zhu
Previous studies have shown aberrant expression of ubiquitin-specific protease 14 (USP14) in multiple malignancies, suggesting an important role of USP14 in tumorigenesis. However, the functional role of USP14 in pancreatic ductal adenocarcinoma (PDAC) has never been elucidated. In this study, we found that USP14 was remarkably upregulated in PDAC tissues compared with normal pancreatic tissues. Notably
-
High nuclear expression of APE1 correlates with unfavorable prognosis and promotes tumor growth in hepatocellular carcinoma J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-04 Xiaohua Lu, Hui Zhao, Hongxin Yuan, Yushan Chu, Xiaoqing Zhu
APE1 is a multifunctional protein that plays important roles in cancer development. However, the association between APE1 expression and the clinicopathological parameters of HCC patients has not been fully characterized. In this study, bioinformatics analysis of APE1 was performed in several databases, including the TCGA, GeneCard, Human Protein Atlas and Ualcan databases. The relationship between
-
Overexpression of T-type calcium channel Cav3.1 in oral squamous cell carcinoma: association with proliferation and anti-apoptotic activity J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-04 Rui-Fang Li, Qi-Wen Man, Jin-Yuan Liu, Yue-Yu Zheng, Xin Gao, Hai-Ming Liu
Cav3.1, a subfamily of T-type calcium channel, is overexpressed in various human cancers and exerts important functions in tumor progression. This study is to identify the expression pattern and clinical significance of Cav3.1 in oral squamous cell carcinoma (OSCC). Firstly, the expression levels of Cav3.1 in oral mucosa (OM), dysplasia and oral squamous cell carcinoma (OSCC) were determined and compared
-
Long non-coding RNA TDRG1 promotes hypoxia-induced glycolysis by targeting the miR-214-5p/SEMA4C axis in cervical cancer cells J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-04 Xiaomei Li, Chunxiao Zhang, Yongju Tian
Long non-coding RNA (lncRNA) has been demonstrated as vital regulator in human cancer. However, the precise role of lnc-TDRG1 in cervical cancer (CC) remains unclear, so this study was aimed to clarify the role and underlying molecular mechanism of lnc-TDRG1 in CC. The real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to assess the expression levels of lnc-TDRG1, miR-214-5p and
-
Polydatin and polydatin-loaded chitosan nanoparticles attenuate diabetic cardiomyopathy in rats J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-03 Fatma Mostafa, Adel Abdel-Moneim, Manal Abdul-Hamid, Sanaa R. Galaly, Hanaa M. Mohamed
-
Salivary exosomes as a new therapy to ameliorate diabetes mellitus and combat xerostomia and submandibular salivary glands dysfunction in diabetic rats J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-03 Zeinab A. Salem, Amany Hany Mohamed Kamel, Nermeen AbuBakr
Diabetes mellitus (DM) is one of the major metabolic diseases. Xerostomia and salivary gland dysfunction are of its common oral complications. Exosomes, as a new therapeutic potential containing nucleic acids, proteins and lipids, act as effective vehicles for target molecules delivery. Accordingly, their therapeutic use is gaining much interest. Therefore, this work aimed to assess the therapeutic
-
MiR-217 promotes endothelial cell senescence through the SIRT1/p53 signaling pathway J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-03 Zhibo Wang, Dianwei Shi, Nan Zhang, Tao Yuan, Huajie Tao
Studies have shown that miR-217 can induce cell senescence, but its mechanism of action in vascular endothelial cell senescence is less reported. Therefore, this study aimed to investigate how miR-217 plays a role in endothelial cell senescence. Human umbilical vein endothelial cells (HUVECs) were used to replicate the aging model, and the population doubling levels (PDLs) during cell passage were
-
LncRNA SNHG1 promotes neuronal injury in Parkinson’s disease cell model by miR-181a-5p/CXCL12 axis J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-03 Hui Wang, Xiaopeng Wang, Yiying Zhang, Jianchuan Zhao
Small molecule RNA host gene 1 (SNHG1) has been found to be an important regulator in the neurotoxicity of Parkinson’s disease (PD). However, the underlying molecular mechanisms of SNHG1 in PD remains elusive. The expression of SNHG1, microRNA (miR)-181a-5p, and C-X-C motif chemokine 12 (CXCL12) mRNA was detected using quantitative real-time polymerase chain reaction. Cell viability and apoptosis were
-
Prognostic significance of LDL receptor-related protein 1B in patients with gastric cancer J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-03 Itaru Yasufuku, Chiemi Saigo, Yusuke Kito, Kazuhiro Yoshida, Tamotsu Takeuchi
LDLR-related protein 1B (LRP1B) is believed to internalize ligands through receptor-mediated endocytosis. Previous epigenetic and genetic studies have indicated that impaired LRP1B mRNA expression might be related to gastric carcinogenesis. However, expression and prognostic significance of LRP1B protein remain to be elucidated. This study aimed to unravel the clinicopathological characteristics of
-
Uremic toxin indoxyl sulfate promotes proinflammatory macrophage activation by regulation of β-catenin and YAP pathways J. Mol. Hist. (IF 2.531) Pub Date : 2021-01-02 Ying Li, Jing Yan, Minjia Wang, Jing Lv, Fei Yan, Jin Chen
Evidence has been shown that indoxyl sulfate (IS) could impair kidney and cardiac functions. Moreover, macrophage polarization played important roles in chronic kidney disease and cardiovascular disease. IS acts as a nephron-vascular toxin, whereas its effect on macrophage polarization during inflammation is still not fully elucidated. In this study, we aimed to investigate the effect of IS on macrophage
-
Coenzyme Q10 enhances remyelination and regulate inflammation effects of cuprizone in corpus callosum of chronic model of multiple sclerosis J. Mol. Hist. (IF 2.531) Pub Date : 2020-11-27 Behnam Khalilian, Soheila Madadi, Nima Fattahi, Beheshteh Abouhamzeh
Multiple Sclerosis (MS) is a chronic, progressive demyelinating disease of the central nervous system that causes the most disability in young people, besides trauma. Coenzyme Q10 (CoQ10)—also known as ubiquinone—is an endogenous lipid-soluble antioxidant in the mitochondrial oxidative respiratory chain which can reduce oxidative stress and inflammation, the processes associated with demyelination
-
NAIP expression increases in a rat model of liver mass restoration J. Mol. Hist. (IF 2.531) Pub Date : 2020-11-25 Julio Plaza-Díaz, Ana I. Álvarez-Mercado, Cándido Robles-Sánchez, Miguel Navarro-Oliveros, Virginia Morón-Calvente, Sofía Toribio-Castelló, María José Sáez-Lara, Alex MacKenzie, Luis Fontana, Francisco Abadía-Molina
The neuronal apoptosis inhibitory protein (NAIP) is a constituent of the NLRC4 inflammasome, which plays a key role in innate immunity, and an antiapoptotic protein. Recently, we reported the previously undescribed role of NAIP in cell division. The liver is one of the body’s most actively regenerative organs. Given the novel mitotic role of NAIP, we examined its expression in hepatic mass restoration
-
SUMO modification in apoptosis J. Mol. Hist. (IF 2.531) Pub Date : 2020-11-22 Peiyao Li, Huiru Jing, Yanzhe Wang, Lei Yuan, Hui Xiao, Qian Zheng
Apoptosis and clearance of dead cells is highly evolutionarily conserved from nematode to humans, which is crucial to the growth and development of multicellular organism. Fail to remove apoptotic cells often lead to homeostasis imbalance, fatal autoimmune diseases, and neurodegenerative diseases. Small ubiquitin-related modifiers (SUMOs) modification is a post-translational modification of ubiquitin
-
Dual roles of TGF-β signaling in the regulation of dental epithelial cell proliferation J. Mol. Hist. (IF 2.531) Pub Date : 2020-11-18 Hao Zhang, Yunyan Zhan, Yue Zhang, Guohua Yuan, Guobin Yang
The purpose of this study is to investigate the molecular mechanisms and biological function of TGF-β-activated Smad1/5 in dental epithelium. Immunohistochemistry was used to detect the expressions of TGF-β signaling-related gene in mice molar germ. Primary dental epithelial cells were cultured and treated with TGF-β1 at a concentration of 0.5 or 5 ng/mL. Small molecular inhibitors, SB431542 and ML347
-
NDRG2 is expressed on enteric glia and altered in conditions of inflammation and oxygen glucose deprivation/reoxygenation J. Mol. Hist. (IF 2.531) Pub Date : 2020-11-17 Yuxin Zhang, Hui Gao, Na Li, Haiqing Chang, Bo Cheng, Yansong Li, Jiwen Miao, Shuang Li, Qiang Wang
Enteric glial cells are more abundant than neurons in the enteric nervous system. Accumulating evidence has demonstrated that enteric glial cells share many properties with astrocytes and play pivotal roles in intestinal diseases. NDRG2 is specifically expressed in astrocytes and is involved in various diseases in the central nervous system. However, no studies have demonstrated the expression of NDRG2
-
Transferrin improved the generation of cardiomyocyte from human pluripotent stem cells for myocardial infarction repair J. Mol. Hist. (IF 2.531) Pub Date : 2020-11-11 Fengzhi Zhang, Hui Qiu, Xiaohui Dong, Chunlan Wang, Jie Na, Jin Zhou, Changyong Wang
Human pluripotent stem cell (hPSC)-derived cardiomyocytes (CMs) hold great promise for the repair of the injured heart, but optimal cell production in a fully chemically defined and cost-effective system is essential for the efficacy and safety of cell transplantation therapies. In this study, we provided a simple and efficient strategy for cardiac differentiation from hPSCs and performed functional
-
Altered BMP-Smad4 signaling causes complete cleft palate by disturbing osteogenesis in palatal mesenchyme J. Mol. Hist. (IF 2.531) Pub Date : 2020-11-07 Nan Li, Jing Liu, Han Liu, Shangqi Wang, Ping Hu, Hailing Zhou, Jing Xiao, Chao Liu
As the major receptor mediated BMP signaling in craniofacial development, Bmpr1a expression was detected in the anterior palatal shelves from E13.5 and the posterior palatal shelves from E14.5. However, inactivating BMP receptor in the mesenchyme only leads to anterior cleft palate or submucous cleft palate. The role of BMP signaling in posterior palatal mesenchyme and palatal osteogenesis is still
-
The role of MYB proto-oncogene like 2 in tamoxifen resistance in breast cancer J. Mol. Hist. (IF 2.531) Pub Date : 2020-11-03 Xia Li, Xi Zhang, Cong-Cong Wu, Ping-Ping Li, Yi-Mou Fu, Li-Hua Xie, Shuang-Shuang Sun, Ying-Ying Zhou, Bao-Ling Zhu
Despite the efficacy of tamoxifen in preventing disease relapse, a large portion of breast cancer patients show intrinsic or acquired resistance to tamoxifen, leading to treatment failure and unfavorable clinical outcome. MYB proto-oncogene like 2 (MYBL2) is a transcription factor implicated in the initiation and progression of various human cancers. However, its role in tamoxifen resistance in breast
-
Regulatory role of insulin-like growth factor-binding proteins in odontogenic mineralization in rats J. Mol. Hist. (IF 2.531) Pub Date : 2020-11-03 Jung-Sun Moon, Yoo-Sung Nam, Jee-Hae Kang, Dong-Wook Yang, Dae-Yoon Kim, Su-Young Lee, Hyun-Mi Ko, Min-Seok Kim, Sun-Hun Kim
Much information is currently available for molecules in early odontogenesis, but there is limited knowledge regarding terminal cytodifferentiation of ameloblasts and odontoblasts for the determination of normal crown morphology. The present differential display PCR (DD-PCR) revealed that insulin-like growth factor-binding protein 5 (IGFBP5) was differentially expressed in molar tooth germs between
-
TRIP13 promotes the proliferation and invasion of lung cancer cells via the Wnt signaling pathway and epithelial–mesenchymal transition J. Mol. Hist. (IF 2.531) Pub Date : 2020-10-30 Zhi-Han Li, Lei Lei, Liang-Ru Fei, Wen-Jing Huang, Yi-Wen Zheng, Mai-Qing Yang, Zhao Wang, Chen-Chen Liu, Hong-Tao Xu
Thyroid hormone receptor interactor 13 (TRIP13) is an ATPase that has been found to be overexpressed in many tumors. The aim of this study was to investigate the role of TRIP13 and its mechanism of action in lung cancer. The expression of TRIP13 was examined in lung cancer tissues and corresponding normal lung tissues by western blotting. TRIP13 was overexpressed or knocked down by transient transfection
-
ELK1 activated-long noncoding RNA LBX2-AS1 aggravates the progression of ovarian cancer through targeting miR-4784/KDM5C axis J. Mol. Hist. (IF 2.531) Pub Date : 2020-10-24 Hangzhi Gu, Rongrong Lin, Feiyun Zheng, Qian Zhang
As one of the most common cancers in female, ovarian cancer (OC) has become a serious public burden now. Mounting researches have indicated long noncoding RNAs (lncRNAs) can affect many biological processes including cancer development. LncRNA LBX2-AS1 was identified to be an oncogene in some cancers, but the role of LBX2-AS1 in OC remains to be elucidated. Bioinformatics analysis and experiments including
-
Metabolic acidosis and hyperkalemia differentially regulate cation HCN3 channel in the rat nephron J. Mol. Hist. (IF 2.531) Pub Date : 2020-10-18 Zinaeli López-González, Teresa Padilla-Flores, Daniel León-Aparicio, Erika Gutiérrez-Vásquez, Carolina Salvador, Juan C. León-Contreras, Rogelio Hernández-Pando, Laura I. Escobar
The kidney controls body fluids, electrolyte and acid–base balance. Previously, we demonstrated that hyperpolarization-activated and cyclic nucleotide-gated (HCN) cation channels participate in ammonium excretion in the rat kidney. Since acid–base balance is closely linked to potassium metabolism, in the present work we aim to determine the effect of chronic metabolic acidosis (CMA) and hyperkalemia
-
MMP7 damages the integrity of the renal tubule epithelium by activating MMP2/9 during ischemia–reperfusion injury J. Mol. Hist. (IF 2.531) Pub Date : 2020-10-17 Qianchao Hu, Jianan Lan, Wenjin Liang, Yiwen Chen, Biao Chen, Zhongzhong Liu, Yan Xiong, Zibiao Zhong, Yanfeng Wang, Qifa Ye
Renal ischemia–reperfusion (IR) injury is a common issue in urological surgery, and the renal tubules, particularly the proximal tubules, are extremely vulnerable to IR injury. In this work, we detected the differently expressed genes (DEGs) between normal rabbit kidneys and IR kidneys by RNA-sequencing, then identified that matrix metalloproteinase–7 (MMP7) played an important role in the progress
-
ITGB1 promotes the chondrogenic differentiation of human adipose-derived mesenchymal stem cells by activating the ERK signaling J. Mol. Hist. (IF 2.531) Pub Date : 2020-10-15 Simin Luo, Qiping Shi, Wuji Li, Wenrui Wu, Zhengang Zha
Adipose-derived mesenchymal stem cell (ADSC) with a high capacity of chondrogenic differentiation was a promising candidate for cartilage defect treatment. This study’s objective is to study the roles of integrin β1 (ITGB1) in regulating ADSC chondrogenic differentiations as well as the underlying mechanisms. The identity of ADSC was confirmed by flow cytometry. ITGB1 gene was overexpressed in human
-
Breast tumour cell subpopulations with expression of the MYC and OCT4 proteins J. Mol. Hist. (IF 2.531) Pub Date : 2020-10-10 N. V. Litviakov, V. A. Bychkov, M. N. Stakheeva, M. K. Ibragimova, M. M. Tsyganov, K. A. Gaptulbarova, L. A. Tashireva, L. N. Bondar, E. Yu. Garbukov, E. M. Slonimskaya
The MYC and OCT4 genes are known factors associated with maintaining pluripotency and are linked with a more aggressive course, progression, and resistance to therapy in cancer. Determining the subpopulations of tumour cells expressing the Myc and Oct4 proteins will provide an opportunity to understand which tumour cell subpopulations expressing MYC and OCT4 are associated with metastasis and resistance
-
Long non-coding RNAs as targets for immunosuppressive drug teriflunomide in anti-cancer potential for hepatocellular carcinoma J. Mol. Hist. (IF 2.531) Pub Date : 2020-10-09 Yinkai Xu, Daoming Shen, Jianxia Liu, Xiaolan Xu, Junhao Tu, Lei Qin, Liyang Jiang, Haixin Qian, Fengbao Guo
Hepatocellular carcinoma (HCC) is the most common form of liver cancer. Because of the relatively chemotherapy-refractory nature of HCC and significant potential poor hepatic reserve, chemotherapy has not been used consistently in the treatment of HCC. Effective new drugs for HCC are urgently needed. Teriflunomide, which was approved for the treatment of relapsing forms of multiple sclerosis (MS),
-
COVID-19 and multiorgan failure: A narrative review on potential mechanisms J. Mol. Hist. (IF 2.531) Pub Date : 2020-10-04 Tahmineh Mokhtari, Fatemeh Hassani, Neda Ghaffari, Babak Ebrahimi, Atousa Yarahmadi, Ghomareza Hassanzadeh
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) in December 2019 form Wuhan, China leads to coronavirus disease 2019 (COVID-19) pandemic. While the common cold symptoms are observed in mild cases, COVID-19 is accompanied by multiorgan failure in severe patients. The involvement of different organs in severe patients results in lengthening the hospitalization duration and
-
Hedgehog pathway activation in oral squamous cell carcinoma: cancer-associated fibroblasts exhibit nuclear GLI-1 localization J. Mol. Hist. (IF 2.531) Pub Date : 2020-09-30 Vanessa Sousa Nazare Guimaraes, Manuela Torres Andion Vidal, Ludmila de Faro Valverde, Marbele Guimarães de Oliveira, Leonardo de Oliveira Siquara da Rocha, Paulo Lucas Cerqueira Coelho, Fernando Augusto Soares, Bruno Solano de Freitas Souza, Daniel Pereira Bezerra, Ricardo D. Coletta, Thiago Almeida Pereira, Jean Nunes dos Santos, Clarissa Araújo Gurgel Rocha
The purpose of this study was to evaluate the expression of Hedgehog (HH) signaling molecules (SHH and GLI-1) by cancer-associated fibroblasts (CAF) in oral squamous cell carcinoma (OSCC). Immunohistochemistry was used to detect molecular HH signaling and CAF-related protein expression, including α-SMA and S100A4, in 70 samples of human OSCC. The colocalization of α-SMA and S100A4 with SHH was also
-
Association of high HIF-1α levels in serous periodontitis with external root resorption by the NFATc1 pathway J. Mol. Hist. (IF 2.531) Pub Date : 2020-09-29 Changjie Xiao, Guangliang Bai, Yanmei Du, Huan Jiang, Xijiao Yu
Whether external root resorption is associated with hypoxia in the periodontal ligaments of teeth with severe periodontitis remains unclear. Hypoxia inducible factor-1α (HIF-1α) expression and external resorption sites in the periodontal ligaments of these teeth were observed to elaborate upon the relationship between hypoxia and external root resorption in severe periodontitis. Histological analysis
-
Lycium barbarum polysaccharide protects against Homocysteine-induced Vascular smooth muscle cell proliferation and phenotypic transformation via PI3K/Akt pathway. J. Mol. Hist. (IF 2.531) Pub Date : 2020-09-08 Minghao Zhang,Fan Li,Smritee Pokharel,Ting Ma,Xiuyu Wang,Yiyong Wang,Weirong Wang,Rong Lin
Lycium barbarum polysaccharide (LBP) is an alkaloid extracted from lycium barbarum. LBP is the active component of lycium barbarum used to treat hypertension, atherosclerosis and other cardiovascular diseases in Chinese traditional medicine. However, the underlying cellular and molecular mechanisms of LBP- mediated activity in vascular disease remain poorly understood. In the present study, we showed
-
Localization of angiotensin-(1-7) and Mas receptor in the rat ovary throughout the estrous cycle. J. Mol. Hist. (IF 2.531) Pub Date : 2020-09-01 Virginia M Pereira,Fernando M Reis,Geovanni D Cassali,Sergio H S Santos,Maira Casalechi,Robson A S Santos,Adelina M Reis
We have previously demonstrated the presence of Angiotensin (Ang)-(1-7) in rat ovary homogenates and its stimulatory effect on estradiol and progesterone production. The present study was undertaken to identify the cellular localization of Ang-(1-7) and its receptor Mas in the rat ovary in the different phases of the estrous cycle. Ang-(1-7) and Mas were localized by immunohistochemistry and Mas mRNA
-
The use of multiplex staining to measure the density and clustering of four endometrial immune cells around the implantation period in women with recurrent miscarriage: comparison with fertile controls. J. Mol. Hist. (IF 2.531) Pub Date : 2020-08-28 Yiwei Zhao,Xiaoyan Chen,Tao Zhang,Loucia K Y Chan,Yingyu Liu,Jacqueline Pui-Wah Chung,Joseph Kwong,Tin-Chiu Li
Serval studies showed an increased uterine natural killer cell density in women with recurrent miscarriage. However, no study has previously investigated the density and clustering of major immune cells simultaneously in precisely timed endometrial specimen section of this group of women. This study aimed to investigate the profile of endometrial immune cells populations and clustering level simultaneously
-
Histidine kinase NME1 and NME2 are involved in TGF-β1-induced HSC activation and CCl4-induced liver fibrosis. J. Mol. Hist. (IF 2.531) Pub Date : 2020-08-28 Hui Gong,Zhiqiang Fan,Dan Yi,Junyu Chen,Zuojun Li,Ren Guo,Chunjiang Wang,Weijin Fang,Shikun Liu
Histidine phosphorylation (pHis) was first reported in 1962. There are few studies on pHis because of the thermal and acidic instability of pHis and the lack of specific methods to detect it. pHis has two isomers of 1-phosphate histidine (1-pHis) and 3-phosphate histidine (3-pHis). pHis antibodies have been developed recently and have promoted research in this field. In this study, we established a
-
Serum LUCAT1 implicates the pathogenesis of muscle-invasive bladder cancer via targeting miR-199a-5p and miR-199b-5p. J. Mol. Hist. (IF 2.531) Pub Date : 2020-08-25 Yong Zhou,Xuejing Song,Xiameng Li,Huimin Li,Ying Peng
Muscle-invasive bladder cancer (MIBC) is a common malignancy of urinary system cancers, accounting for about 1/3 of all newly diagnosed bladder cancer cases. Due to its strong metastasis, the 5-year survival of MIBC is less than 50%, and in serious cases, the overall survival of metastatic bladder cancer patients is about 1.3 years. LncRNAs, a type of non-coding RNAs defined as the transcripts exceeding
-
Down-regulation of SETD6 protects podocyte against high glucose and palmitic acid-induced apoptosis, and mitochondrial dysfunction via activating Nrf2-Keap1 signaling pathway in diabetic nephropathy. J. Mol. Hist. (IF 2.531) Pub Date : 2020-08-16 Xiang Wang,Qiling Liu,Deqin Kong,Zi Long,YuFang Guo,Shuang Wang,Rui Liu,Chunxu Hai
-
Lipid mass spectrometry imaging and proteomic analysis of severe aortic stenosis. J. Mol. Hist. (IF 2.531) Pub Date : 2020-08-13 Jihyeon Lim,Jennifer T Aguilan,Rani S Sellers,Fnu Nagajyothi,Louis M Weiss,Ruth Hogue Angeletti,Anna E Bortnick
Severe aortic stenosis (AS) is prevalent in adults ≥ 65 years, a significant cause of morbidity and mortality, with no medical therapy. Lipid and proteomic alterations of human AS tissue were determined using mass spectrometry imaging (MSI) and liquid chromatography electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS) to understand histopathology, potential biomarkers of disease, and progression
-
A new approach for examining the neurovascular structure with phalloidin and calcitonin gene-related peptide in the rat cranial dura mater. J. Mol. Hist. (IF 2.531) Pub Date : 2020-08-13 Jia Wang,Dongsheng Xu,Jingjing Cui,Shuya Wang,Chen She,Hui Wang,Shuang Wu,Jianliang Zhang,Bing Zhu,Wanzhu Bai
The neurovascular structures in the cranial dura mater have been studied with various histological techniques in the past years. In order to obtain a proper approach to reveal the detailed structures, different labeling methods for the cranial vessels and nerve fibers were tested in this study. Firstly, the labeling characteristics of phalloidin, alpha smooth muscle actin (α-SMA), and CD31 were compared
-
Overexpression of PREX1 in oral squamous cell carcinoma indicates poor prognosis. J. Mol. Hist. (IF 2.531) Pub Date : 2020-08-12 Shu-Cheng Wan,Hao Wu,Hao Li,Wei-Wei Deng,Yao Xiao,Cong-Cong Wu,Lei-Lei Yang,Wen-Feng Zhang,Zhi-Jun Sun
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger (P-Rex) proteins control many fundamental cellular functions including cell migration, actin cytoskeletal rearrangement and adhesion in many cancers. However, P-Rex1 expression and its prognostic effect and possible clinical value are not clearly elucidated in human oral squamous cell carcinoma (OSCC). Here, OSCC tissue microarrays were
-
Spatiotemporal expression of neurogenic locus notch homolog protein 1 in developing caudal spinal cord of fetuses with anorectal malformations from ETU-fed rats. J. Mol. Hist. (IF 2.531) Pub Date : 2020-08-11 Zhonghua Yang,Huimin Jia,Yuzuo Bai,Weilin Wang
Complications, such as fecal soiling, incontinence, and constipation, are major health issues for patients with anorectal malformations (ARMs) after surgery. Dysplasia of the caudal spinal cord is an increasingly pivotal area in the field of postoperative complications for patients with ARMs. However, the existing research has not fully defined the mechanism underlying ARMs development. The neurogenic
-
Mechanical adaptation of synoviocytes A and B to immobilization and remobilization: a study in the rat knee flexion model. J. Mol. Hist. (IF 2.531) Pub Date : 2020-08-10 Haodong Zhou,Guy Trudel,Odette Laneuville
The objective of this study was to quantify the in vivo response of synoviocytes type A and B in the posterior joint capsule to knee immobilization and remobilization. Also, to correlate the immunohistochemical data with selected mRNA expression in the posterior joint capsule. Forty-two adult male Sprague–Dawley rats had one knee joint immobilized in flexion for durations of 1–4 weeks. Fifteen were
-
The roles of galectins in hepatic diseases. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-31 Mei-Juan Sun,Zhan-Qi Cao,Ping Leng
Hepatic diseases include all diseases that occur in the liver, including hepatitis, cirrhosis, hepatocellular carcinoma, etc. Hepatic diseases worldwide are characterized by high incidences of digestive system diseases, which present with subtle symptoms, are difficult to treat and have high mortality. Galectins are β-galactoside-binding proteins that have been found to be aberrantly expressed during
-
The anticonvulsant effect of sparteine on pentylenetetrazole-induced seizures in rats: a behavioral, electroencephalographic, morphological and molecular study. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-29 Fridha Villalpando-Vargas,Laura Medina-Ceja,Anne Santerre,Edgar A Enciso-Madero
Abnormal synchronous activity in neurons generates epileptic seizures. Antiepileptic drugs (AEDs) are effective in 70% of patients, but this percentage is drastically lower in developing countries. Sparteine is a quinolizidine alkaloid synthesized from most Lupine species and has a probable anticonvulsive effect. For this reason, the objective of the present work was to study the anticonvulsant effect
-
Effects of titanium dioxide nanoparticles on the myocardium of the adult albino rats and the protective role of β-carotene (histological, immunohistochemical and ultrastructural study). J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-15 Emtethal M El-Bestawy,Asmaa M Tolba
Titanium dioxide nanoparticles (TiO2 NPs) are the most produced nanomaterials. TiO2 NPs are used as a drug carrier and molecular imaging vehicle in the cardiovascular system. We aimed to study TiO2 NPs effects on the ventricular myocardium and evaluate the ameliorative effects of β-carotene (βC). Forty adult albino rats were divided into four groups: negative control group (Ι) received a distilled
-
Correction to: CircUBAP2 promotes SEMA6D expression to enhance the cisplatin resistance in osteosarcoma through sponging miR-506-3p by activating Wnt/β-catenin signaling pathway. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-14 Xuelian Gong,Wei Li,Lin Dong,Fangfei Qu
The authors of the article want to add Xuelian Gong and Wei Li as the first author and co-first author as they have greatly contributed to the revision work. The correct author group is given in this Correction.
-
Exosomes derived from human exfoliated deciduous teeth ameliorate adult bone loss in mice through promoting osteogenesis. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-12 Jizhen Wei,Yeqing Song,Zhihao Du,Feiyan Yu,Yimei Zhang,Nan Jiang,Xuejun Ge
Cell-free based therapy is an effective strategy in regenerative medicine as it avoids controversial issues, such as immunomodulation and stability. Recently, exosomes have been explored as a favorable substitution for stem cell therapy as they exhibit multiple advantages, such as the ability to be endocytosed and innate biocompatibility. This study aimed to investigate the effects of stem cells from
-
Quantitative characterization of proliferative cells subpopulations in the hilus of the hippocampus of adult Wistar rats: an integrative study. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-11 Yuliana García-Martinez,Karla Berenice Sánchez-Huerta,Jorge Pacheco-Rosado
The hilus plays an important role modulating the excitability of the hippocampal dentate gyrus (DG). It also harbors proliferative cells whose proliferation rate is modified during pathological events. However, the characterization of these cells, in terms of cellular identity, lineage, and fate, as well as the morphology and proportion of each cell subpopulation has been poorly studied. Therefore
-
Interaction between C/EBPβ and RUNX2 promotes apoptosis of chondrocytes during human lumbar facet joint degeneration. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-06 Jinlong Zhang,Jiawei Jiang,Guofeng Bao,Guanhua Xu,Lingling Wang,Jiajia Chen,Chu Chen,Chunshuai Wu,Pengfei Xue,Dawei Xu,Yuyu Sun,Zhiming Cui
The pathophysiological changes in cartilage are a crucial feature of lumbar facet joint (LFJ) degeneration and arthritis. However, the molecular mechanism of human LFJ degeneration remains largely defined. This study aimed to examine the changes in chondrocytes at different stages of degenerative LFJ using hematoxylin and eosin and Safranin O staining. The significant loss of chondrocytes in grades
-
Correction to: Adipose mesenchymal stem cell exosomes promote wound healing through accelerated keratinocyte migration and proliferation by activating the AKT/HIF-1α axis. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-02 Yue Zhang,Fei Han,Lan Gu,Peng Ji,Xuekang Yang,Mengdong Liu,Ke Tao,Dahai Hu
In the original publication of the article, the name of one of the corresponding authors was published incorrectly. The name should be 'Ke Tao' instead of 'Kao Tao'. The corrected author group is given in this Correction.
-
Advillin is a tuft cell marker in the mouse alimentary tract. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-02 Anna-Lena Ruppert,Maryam Keshavarz,Sarah Winterberg,Johannes Oberwinkler,Wolfgang Kummer,Burkhard Schütz
Tuft cells are a rare population of chemosensory cells at the mucosal surface epithelia of hollow organs. Their name-giving morphological feature is an apical tuft of stiff microvilli. Accordingly, the actin-binding protein, villin, was identified as one of the first tuft cell markers in immunohistochemical analysis. Unfortunately, villin expression is not restricted to tuft cells, but is also prominent
-
DNA repair and cell synthesis proteins: immunohistochemical expression and correlation with recurrence-regrowth in meningiomas. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-02 Camila Batista de Oliveira Silva,Bárbara Roberta Ongaratti,Geraldine Trott,Bruna Araújo,Carolina Soares Leães Rech,Lígia Barbosa Coutinho,Nelson Pires Ferreira,Miriam da Costa Oliveira,Júlia Fernanda Semmelmann Pereira-Lima
Meningiomas are considered the second most common neoplasm of the central nervous system in adults. Most of them are benign with slow growth, frequent in women and with a high recurrence rate. In tumors, DNA error repair processes lose efficacy, providing mutagenesis and genomic instability. This work evaluated the expression of proteins involved in cell synthesis (cyclin D1) and DNA errors repair
-
Correction to: Downregulation of the long noncoding RNA TUG1 inhibits the proliferation, migration, invasion and promotes apoptosis of renal cell carcinoma. J. Mol. Hist. (IF 2.531) Pub Date : 2020-07-01 Meng Zhang,Wei Lu,Yiqiang Huang,Jizhou Shi,Xun Wu,Xiaolong Zhang,Runze Jiang,Zhiming Cai,Song Wu
In the original article, we realized that an error occurred when we grouped the separate photos to generate the combined Figure 4 The same image was accidentally pasted twice in one figure, while we were not fully aware of the error at that time. We immediately reviewed the original data again and made sure that no changes need to be made in other parts of the paper. We are sure that the published
-
Integrin-linked kinase (ILK) regulates KRAS, IPP complex and Ras suppressor-1 (RSU1) promoting lung adenocarcinoma progression and poor survival. J. Mol. Hist. (IF 2.531) Pub Date : 2020-06-22 Sofia Nikou,Marina Arbi,Foteinos-Ioannis D Dimitrakopoulos,Chaido Sirinian,Panagiota Chadla,Ioanna Pappa,Giannoula Ntaliarda,Georgios T Stathopoulos,Helen Papadaki,Vasiliki Zolota,Zoi Lygerou,Haralabos P Kalofonos,Vasiliki Bravou
Integrin-linked kinase (ILK) forms a heterotrimeric protein complex with PINCH and PARVIN (IPP) in Focal Adhesions (FAs) that acts as a signaling platform between the cell and its microenvironment regulating important cancer-related functions. We aimed to elucidate the role of ILK in lung adenocarcinoma (LUADC) focusing on a possible link with KRAS oncogene. We used immunohistochemistry on human tissue
Contents have been reproduced by permission of the publishers.