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Trypanosoma brucei transferrin receptor: functional replacement of the GPI anchor with a transmembrane domain Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2021-01-12 Mostafa Kabiri; Dietmar Steverding
The transferrin receptor of Trypanosoma brucei (TbTfR) is a heterodimer of a glycosylphosphatidylinositol (GPI)-anchored ESAG6 subunit and an ESAG7 subunit. To investigate whether the GPI-anchor is essential for the function of the TbTfR, an ESAG6 with a transmembrane domain instead of a GPI-anchor (ESAG6tmd) was inducibly expressed in bloodstream form trypanosomes. It is shown that the ESAG6tmd is
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Low molecular weight protein tyrosine phosphatase (LMW-PTP2) protein can potentially modulate virulence of the parasite Entamoeba histolytica Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2021-01-09 Diana Milena Torres-Cifuentes; Patricia Espíritu-Gordillo; Lidia Baylón-Pacheco; José Luis Rosales-Encína
The Entamoeba histolytica parasite is the causative agent of amebiasis, infecting approximately 1% of the world population and causing 100,000 deaths per year. It binds to Fibronectin (FN), activating signaling pathways regulated by kinases and phosphatases. EhLMW-PTPs genes from E. histolytica encode for Low Molecular Weight Tyrosine Phosphatases expressed in trophozoites and amoebic cysts. The role
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Inhibition of inflammatory cytokine production and proliferation in macrophages by Kunitz-type inhibitors from Echinococcus granulosus Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2021-01-08 Camila Sagasti; Cecilia Casaravilla; Cecilia Fernández; Martín Fló; Álvaro Díaz
The genus Echinococcus of cestode parasites includes important pathogens of humans and livestock animals. Transcriptomic and genomic studies on E. granulosus and E. multilocularis uncovered striking expansion of monodomain Kunitz proteins. This expansion is accompanied by the specialization of some family members away from the ancestral protease inhibition function to fulfill cation channel blockade
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Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2021-01-07 Strahinja Stevanovic; Djordje S. Marjanović; Sasa M. Trailović; Nemanja Zdravković; Andrej Perdih; Katarina Nikolic
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Formation of mRNP granules in Toxoplasma gondii during the lytic cycle Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-12-28 Scott Roscoe; Emad Manni; Mikayla Roberts; Sirinart Ananvoranich
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VSG mRNA levels are regulated by the production of functional VSG protein Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-12-19 Isabella E. Maudlin; Steve Kelly; Angela Schwede; Mark Carrington
The bloodstream form of Trypanosoma brucei persists in mammalian hosts through a population survival strategy depending on antigenic variation of a cell surface coat composed of the variant surface glycoprotein (VSG). The integrity of the VSG coat is essential and blocking its synthesis results in a cell division cycle arrest just prior to cytokinesis. This observation indicates that VSG levels are
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Gliding motility protein LIMP promotes optimal mosquito midgut traversal and infection by Plasmodium berghei Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-12-18 Saskia Egarter; Jorge M. Santos; Jessica Kehrer; Julia Sattler; Friedrich Frischknecht; Gunnar R. Mair
Substrate-dependent gliding motility is key to malaria transmission. It mediates host cell traversal, invasion and infection by Plasmodium and related apicomplexan parasites. The 110 amino acid-long cell surface protein LIMP is essential for P. berghei sporozoites where it is required for the invasion of the mosquito’s salivary glands and the liver cells of the rodent host. Here we define an additional
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Generation of Plasmodium yoelii malaria parasite for conditional degradation of proteins Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-12-08 Chuanyuan Liu; Zhenke Yang; Mengya Cai; Yang Shi; Huiting Cui; Jing Yuan
The auxin-inducible degron (AID) system is a robust chemical-genetic method for manipulating endogenous protein level by conditional proteasomal degradation via a small molecule. So far, this system has not been adapted in the P. yoelii, an important and widely used Plasmodium rodent parasite model for malaria biology. Here, using the CRISPR/Cas9 genome editing method, we generated two marker-free
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Behavioral and molecular response of the insect parasitic nematode Steinernema carpocapsae to cues emitted by a host, the red palm weevil, Rhynchophorus ferrugineus Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-12-05 Velayudhan Satheeja Santhi; Dana Ment; Adi Faigenboim; Liora Salame; Victoria Soroker; Amots Hetzroni; Itamar Glazer
As the larvae of the date palm pest, the red palm weevil, Rhynchophorus ferrugineus, feeds on the host tissue, they emit a distinctive sound which can be recorded outside of the infected tree. We evaluated the response of infective juveniles (IJs) of the entomopathogenic nematodes Steinernema carpocapsae to the R. ferrugineus larvae and it’s sound source, separately. In the presence of the insect larvae
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Testing the CRISPR-Cas9 and glmS ribozyme systems in Leishmania tarentolae Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-11-06 Gino L. Turra; Luzia Schneider; Linda Liedgens; Marcel Deponte
Leishmania parasites include important pathogens and model organisms and are even used for the production of recombinant proteins. However, functional genomics and the characterization of essential genes are often limited in Leishmania because of low-throughput technologies for gene disruption or tagging and the absence of components for RNA interference. Here, we tested the T7 RNA polymerase-dependent
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Expression of Heat shock protein 70 (Hsp70-1) in Plasmodium berghei ookinetes and its participation in midgut mosquito infection Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-11-02 Maria Carmen Rodriguez; Jesús Martínez-Barnetche; Alba N. Lecona-Valera; Juan Téllez-Sosa; Rocio S. Argotte-Ramos; Alejandro Alvarado-Delgado; Marbella T. Ovilla; Vianey Saldaña-Navor; Mario H. Rodriguez
The heat shock protein family 70 (Hsp70) comprises chaperone proteins that play major multiple roles in Plasmodium asexual and sexual development. In this study, we analyzed the expression of Hsp70-1 in gametocytes, gametes, zygotes, and its participation in ookinete formation and their transition into oocysts. A monoclonal antibody against recombinant Hsp70-1 revealed its presence in zygotes and micronemes
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Characterisation and structural analysis of glyoxylate cycle enzymes of Teladorsagia circumcincta Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-10-13 Saleh Umair; Charlotte Bouchet; Nikola Palevich; Heather V. Simpson
A 1332 bp full length cDNA encoding Teladorsagia circumcincta isocitrate lyase (TciICL) and a 1575 bp full length cDNA encoding T. circumcincta malate synthase (TciMS) were cloned, expressed in Escherichia coli and the recombinant proteins purified. The predicted TciICL protein of 444 amino acids was present as a single band of about 52 kDa on SDS-PAGE and the recombinant TciMS of 525 amino acids formed
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Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimeria necatrix. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-18 Dandan Liu,Feiyan Wang,Liqin Cao,Lele Wang,Shijie Su,Zhaofeng Hou,Jinjun Xu,Junjie Hu,Jianping Tao
Gametocyte proteins of Eimeria spp. are essential components of the oocyst wall, and some of these proteins have been analysed to identify targets of transmission-blocking vaccines against avian coccidiosis. In the present study, a cDNA from E. necatrix gametocytes was cloned and sequenced. The cDNA is 1473 bp in length and encodes a 490-amino-acid protein containing a tyrosine–serine (Tyr/Ser)-rich
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Two phylogenetically divergent isocitrate dehydrogenases are encoded in Leishmania parasites. Molecular and functional characterization of Leishmania mexicana isoenzymes with specificity towards NAD+ and NADP+ Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-24 Lucila Giordana; Cristina Nowicki
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Characterization of natural antisense transcripts arisen from the locus encoding Toxoplasma gondii ubiquitin-like protease Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-10-01 Ambreen Fahim; Farzana Afrin; Grace Wen; Sirinart Ananvoranich
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Lipid metabolism in Trypanosoma cruzi: a review. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-20 Leigh-Ann Booth,Terry K Smith
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Characterization of glycan determinants that mediate recognition of the major Wuchereria bancrofti circulating antigen by diagnostic antibodies. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-20 Marla Hertz,Amy Rush,Thomas B Nutman,Gary Weil,Sasisekhar Bennuru,Philip Budge
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A comparative proteomics analysis of the egg secretions of three major schistosome species. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-19 Jack P Carson,Mark W Robinson,Michael H Hsieh,James Cody,Loc Le,Hong You,Donald P McManus,Geoffrey N Gobert
Morbidity associated with hepatic and urogenital schistosomiasis stems primarily from the host immune response directed against schistosome eggs. When eggs become entrapped in host tissues, the development of fibrotic plaques drives downstream pathology. These events occur due to the antigenic nature of egg excretory/secretory products (ESPs). Both Schistosoma mansoni and S. japonicum ESPs have been
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Production and characterization of monoclonal antibodies against highly immunogenic Opisthorchis viverrini proteins and development of coproantigen detection Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-24 Yuji Arimatsu; Salma Teimoori; Arpa Surapaitoon; Banchob Sripa
Opisthorchis viverrini and other foodborne trematode infections are major health concerns in the Greater Mekong Subregion. Currently, the gold-standard diagnostic method for opisthorchiasis is conventional stool examination for the presence of parasite eggs. This method lacks sensitivity and needs an experienced technician. We therefore produced monoclonal antibodies to highly immunogenic O. viverrini
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Molecular basis of inhibition of Schistosoma japonicum glutathione transferase by ellagic acid: insights into biophysical and structural studies. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-19 Blessing O Akumadu,Ramesh Pandian,Jessica Olfsen,Roland Worth,Monare Thulo,Tshireletso Mentor,Sylvia Fanucchi,Yasien Sayed,Heini W Dirr,Ikechukwu Achilonu
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Characterization and evaluation of three new recombinant antigens of Taenia solium for the immunodiagnosis of cysticercosis. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-19 Moraima Morillo,Cynthia Noguera,Lina Gallego,Zeidali Fernández,Marianny Mata,Sajar Khattar,María Lares,Teresa Gárate,Elizabeth Ferrer
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The endoplasmic reticulum-resident serpentine receptor SR10 has important functions for asexual and sexual blood stage development of Plasmodium falciparum. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-02 Emilie Joëlle Njila Tchoufack,Lena Hahnfeld,Georg Pitschelatow,Sandra Bennink,Gabriele Pradel
Serpentine receptors (SRs) are transmembrane proteins generally acting as mediators to facilitate the communication between a cell and its environment. At least six putative SR-like proteins are encoded in the genome of the malaria parasite Plasmodium falciparum. For two of them, roles in cell stress control were reported; however, for most of the SR-like proteins the functions are not yet known. In
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Characterisation of a Teladorsagia circumcincta glutathione transferase. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-09-01 Saleh Umair,Charlotte L G Bouchet,Qing Deng,Nikola Palevich,Heather V Simpson
A 615 bp full length cDNA encoding a Teladorsagia circumcincta glutathione transferase (TcGST) was cloned, expressed in Escherichia coli and the recombinant protein purified and its kinetic properties determined. The predicted protein consisted of 205 amino acids and was present as a single band of about 24 kDa on SDS-PAGE. Multiple alignments of the protein sequence of TcGST with homologues from other
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Diagnostic urinary cfDNA detected in human cystic echinococcosis. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-08-28 Luz Toribio,Saul Santivanez,Alan L Scott,Raul Enriquez,Cesar Sedano,Percy Soto-Becerra,Hector H Garcia,Clive J Shiff,
Cystic echinococcosis (CE) is a major neglected tropical zoonotic disease caused by the tissue-dwelling larval stage of the cestode parasite Echinococcus granulosus. For individuals suspected of CE, the diagnostic standard is imaging using ultrasonography, X rays, or computed tomography. These resource-demanding and expensive procedures are rarely available in endemic rural areas where CE is most prevalent
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The highly diverse TATA box-binding proteins among protists: A review. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-08-06 Olivia Parra-Marín,Karla López-Pacheco,Roberto Hernández,Imelda López-Villaseñor
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Proteomic analysis of Ascocotyle longa (Trematoda: Heterophyidae) metacercariae. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-08-01 Karina M Rebello,Juliana N Borges,André Teixeira,Jonas Perales,Cláudia P Santos
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p97 Inhibitor CB-5083 Blocks ERAD in Trypanosoma brucei. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-07-28 Paige Garrison,James D Bangs
Misfolded proteins trapped in the endoplasmic reticulum (ER) are specifically recognized and retrotranslocated to the cytosol by the ER-Associated Degradation (ERAD) system and delivered to the proteasome for destruction. This process was recently described in Trypanosoma brucei (T. brucei) using the misfolded epitope tagged Transferrin Receptor subunits ESAG7:Ty and HA:ESAG6 (HA:E6). Critical to this
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A lysosomal hydrolase receptor, CPBF2, is associated with motility and invasion of the enteric protozoan parasite Entamoeba histolytica. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-07-21 Kumiko Nakada-Tsukui,Konomi Marumo,Tomoyoshi Nozaki
Proper targeting and secretion of lysosomal hydrolases are regulated by transporting receptors. Entamoeba histolytica, the enteric protozoan parasite responsible for human amebiasis, has a unique family of lysosomal hydrolase receptors, cysteine protease binding protein family, CPBF. CPBFs, consisting of 11 members with conserved domain organization, bind to a wide range of cargos including cysteine
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Direct and high-throughput assays for human cell killing through trogocytosis by Entamoeba histolytica. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-07-17 Akhila Bettadapur,Katherine S Ralston
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A rapid approach for in locus overexpression of Trypanosoma brucei proteins. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-07-16 Hana Vachova,Glenda Alquicer,Miroslava Sedinova,Jana Sachova,Miluse Hradilova,Vladimir Varga
Altering amounts of a protein in a cell has become a crucial tool for understanding its function. In many organisms, including the protozoan parasite Trypanosoma brucei, protein overexpression has been achieved by inserting a protein-coding sequence into an overexpression vector. Here, we have adapted the PCR only based system for tagging trypanosome proteins at their endogenous loci such that it in
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Bar-seq strategies for the LeishGEdit toolbox. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-07-10 Tom Beneke,Eva Gluenz
The number of fully sequenced genomes increases steadily but the function of many genes remains unstudied. To accelerate dissection of gene function in Leishmania spp. and other kinetoplastids we previously developed a streamlined pipeline for CRISPR-Cas9 gene editing, which we termed LeishGEdit. To facilitate high-throughput mutant screens we have adapted this pipeline by barcoding mutants with unique
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Nematode endosymbiont competition: Fortune favors the fittest. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-07-01 Christa Heryanto,Ioannis Eleftherianos
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Senna plant generates reactive oxygen species (ROS) and induces apoptosis in Hymenolepis diminuta. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-06-30 Saptarshi Roy,Nikhilesh Joardar,Santi P Sinha Babu,Larisha M Lyndem
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Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-06-27 Michiel L Bexkens,Renske A van Gestel,Bas van Breukelen,Rolf T Urbanus,Jos F Brouwers,Rienk Nieuwland,Aloysius G M Tielens,Jaap J van Hellemond
Eggs, schistosomula and adult Schistosoma worms are known to release extracellular vesicles (EV) during in vitro incubations and these EVs are postulated to affect the host responses. So far only those EVs released during in vitro incubations of schistosomes have been studied and it is unknown whether in blood of infected hosts the schistosomal EVs can be detected amidst all the circulating EVs of
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Analysis of codon usage pattern of mitochondrial ND genes in Platyhelminthes. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-06-24 Gulshana A Mazumder,Arif Uddin,Supriyo Chakraborty
Codon usage bias (CUB) is the nonrandom usage of synonymous codons in which some codons are more preferred to others.CUB can be determined by mutation pressure and selection. Various approaches have been used to understand the pattern of CUB in the mitochondrial ND (MT-ND or ND) genes involved in complex I of respiratory chain in five different classes of Platyhelminthes as no work was reported yet
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Effect of Posaconazole in an in vitro model of cardiac fibrosis induced by Trypanosoma cruzi. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-06-18 Lindice Mitie Nisimura,Patrícia Mello Ferrão,Alanderson da Rocha Nogueira,Mariana Caldas Waghabi,Marcelo Meuser-Batista,Otacílio C Moreira,Julio A Urbina,Luciana Ribeiro Garzoni
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The Entamoeba lysine and glutamic acid rich protein (KERP1) virulence factor gene is present in the genomes of Entamoeba nuttalli, Entamoeba dispar and Entamoeba moshkovskii. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-06-11 Gareth D Weedall
The lysine and glutamic acid rich protein KERP1 is a cell surface-expressed virulence factor in the human pathogen Entamoeba histolytica. It was originally suggested that the gene was absent from the related, avirulent human commensal Entamoeba dispar, an absence which would be relevant to the differential virulence of these species. Here, the gene is shown to be present in E. dispar, and its sequence
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Use of split-dihydrofolate reductase for the detection of protein-protein interactions and simultaneous selection of multiple plasmids in Plasmodium falciparum. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-06-04 Yvette S Levray,Anne D Berhe,Andrew R Osborne
Defining protein-protein interactions is fundamental to the understanding of gene function. Protein-fragment complementation assays have been used for the analysis of protein-protein interactions in various organisms. The split-dihydrofolate reductase (DHFR) protein-fragment complementation assay utilises two complementary fragments of the enzyme fused to a pair of potentially interacting proteins
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Structural and biochemical analysis of phosphoethanolamine methyltransferase from the pine wilt nematode Bursaphelenchus xylophilus. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-05-30 Soon Goo Lee,Michelle S Chung,Antea J DeMarsilis,Cynthia K Holland,Rohit V Jaswaney,Cherry Jiang,Jakob H P Kroboth,Kevin Kulshrestha,Raymundo Z W Marcelo,Vidhya M Meyyappa,Grant B Nelson,Janki K Patel,Alex J Petronio,Samantha K Powers,Peter R Qin,Mythili Ramachandran,Divya Rayapati,John A Rincon,Andreia Rocha,Joan Gabriel Rodinho Nunes Ferreira,Micah K Steinbrecher,Kaisen Yao,Eric J Zhang,Angela J
In free-living and parasitic nematodes, the methylation of phosphoethanolamine to phosphocholine provides a key metabolite to sustain phospholipid biosynthesis for growth and development. Because the phosphoethanolamine methyltransferases (PMT) of nematodes are essential for normal growth and development, these enzymes are potential targets of inhibitor design. The pine wilt nematode (Bursaphelenchus
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Comparative study of transcription profiles of the P-glycoprotein transporters of two Haemonchus contortus isolates: Susceptible and resistant to ivermectin. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-05-17 David E Reyes-Guerrero,Marilyn Cedillo-Borda,Rogelio A Alonso-Morales,Miguel A Alonso-Díaz,Agustín Olmedo-Juárez,Pedro Mendoza-de-Gives,Ma Eugenia López-Arellano
The objective of this study was to analyze the mRNA transcription levels of ten functional genes of P-glycoproteins (P-gp) in free life stages, eggs and infective larvae (L3) and in endoparasitic stages, fourth larval stage (L4) and adult males of two native isolates of Haemonchus contortus: resistant and susceptible to IVM. The IVM resistant isolate was obtained from sheep naturally infected with
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Actin sequestering protein, profilin, regulates intracellular vesicle transport in Leishmania. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-05-12 Bindu Ambaru,Anupriya Gopalsamy,T V Satish Tammana,Hosahalli S Subramanya,Chhitar M Gupta
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Orientation of FtsH protease homologs in Trypanosoma brucei inner mitochondrial membrane and its evolutionary implications. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-05-11 Tomáš Kovalinka,Tomáš Pánek,Bianka Kováčová,Anton Horváth
Trypanosoma brucei is an important human pathogen. In this study, we have focused on the characterization of FtsH protease, ATP-dependent membrane-bound mitochondrial enzyme important for regulation of protein abundance. We have determined localization and orientation of all six putative T.brucei FtsH homologs in the inner mitochondrial membrane by in silico analyses, by immunofluorescence, and with
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Vancomycin improves Plasmodium yoelii malaria parasite in vitro liver stage cultures by controlling Elizabethkingia anophelis, a bacterium in the microbiome of lab-reared Anopheles mosquitoes. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-04-30 Melanie J Shears,Sean C Murphy
Studies of Plasmodium sporozoites and liver stages require dissection of Anopheles mosquitoes to obtain sporozoites for experiments. Sporozoites from the rodent parasite P. yoelii are routinely used to infect hepatocytes for liver stage culture, but sometimes these cultures become contaminated. Using standard microbiological techniques, a single colony type of Gram-negative rod-shaped bacteria was
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The complexity and diversity of the actin cytoskeleton of trypanosomatids. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-04-28 Andrea Vizcaíno-Castillo,Juan Felipe Osorio-Méndez,Javier R Ambrosio,Roberto Hernández,Ana María Cevallos
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The mitochondrial phosphate carrier TbMCP11 is essential for mitochondrial function in the procyclic form of Trypanosoma brucei. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-04-27 Fei Gao,Frank Voncken,Claudia Colasante
Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, were predicted to function as phosphate importers. The transport of inorganic phosphate
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Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-04-26 Samantha Nava,Aygul Sadiqova,Alejandro Castellanos-Gonzalez,A Clinton White
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Isolation and characterization of a novel member of the ACC ligand-gated chloride channel family, Hco-LCG-46, from the parasitic nematode Haemonchus contortus. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-04-06 Sarah A Habibi,Stephen M Blazie,Yishi Jin,Sean G Forrester
The ACC-1 family of cys-loop receptors are ligand-gated chloride channels sensitive to acetylcholine (ACh), and are only present in invertebrates. Studies of this family of inhibitory receptors has provided insight into how they bind and respond to ACh in a manner vastly different from nicotinic acetylcholine receptors and appear to be present in tissues that are relevant to anthelmintic action. Here
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Characterization of the complete mitogenome of Centrorhynchus clitorideus (Meyer, 1931) (Palaeacanthocephala: Centrorhynchidae), the largest mitochondrial genome in Acanthocephala, and its phylogenetic implications. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-03-31 Nehaz Muhammad,Suleman,Mian Sayed Khan,Liang Li,Qing Zhao,Hanif Ullah,Xing-Quan Zhu,Jun Ma
Species of Centrorhynchus (Polymorphida: Centrorhynchidae) commonly parasitize various falconiform and strigiform birds worldwide. In the present study, the complete mitochondrial (mt) genome sequences of Centrorhynchus clitorideus was sequenced and annotated for the first time based on specimens collected from the little owl Athene noctua (Scopoli) (Strigiformes: Strigidae) in Pakistan. The complete
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Structural and kinetic characterization of Trypanosoma congolense pyruvate kinase. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-02-19 Joar Esteban Pinto Torres,Meng Yuan,Julie Goossens,Wim Versées,Guy Caljon,Paul A Michels,Malcolm D Walkinshaw,Stefan Magez,Yann G-J Sterckx
Trypanosoma are blood-borne parasites and are the causative agents of neglected tropical diseases (NTDs) affecting both humans and animals. These parasites mainly rely on glycolysis for their energy production within the mammalian host, which is why trypanosomal glycolytic enzymes have been pursued as interesting targets for the development of trypanocidal drugs. The structure–function relationships
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YihA GTPases localize to the apicoplast and mitochondrion of the malaria parasite and interact with LSU of organellar ribosomes. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-02-11 Ankit Gupta,Kirti Gupta,Saman Habib
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The recognition of proteasomal receptors by Plasmodium falciparum DSK2. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-02-11 Ishita Gupta,Sameena Khan
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Molecular basis for hycanthone drug action in schistosome parasites. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-02-03 Meghan Guzman,Anastasia Rugel,Reid S Tarpley,Xiaohang Cao,Stanton F McHardy,Philip T LoVerde,Alexander B Taylor
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Corrigendum to "Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host-parasite interaction and novel targets for diagnostic" [Mol. Biochem. Parasitol. 235 (2020) 111249]. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-02-03 Priscilla D M Fonseca,Marcelo A Corral,Miguel Cosenza-Contreras,Dirce M C L Meisel,Gessica B Melo,Milena M S Antunes,Maria C E Santo,Ronaldo C B Gryschek,Julia M Costa-Cruz,William Castro-Borges,Fabiana M Paula
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Proteomic analysis of two populations of Schistosoma mansoni-derived extracellular vesicles: 15k pellet and 120k pellet vesicles. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-01-31 Desalegn Woldeyohannes Kifle,Mark S Pearson,Luke Becker,Darren Pickering,Alex Loukas,Javier Sotillo
Helminth parasites secrete extracellular vesicles (EVs) into their environment that have potential roles in host-parasite communication, and thus represent potentially useful targets for novel control strategies. Here, we carried out a comprehensive proteomic analysis of two different populations of EVs – 15k pellet and 120k pellet EVs – from Schistosoma mansoni adult worms. We characterised the proteins
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Observation of morphological changes of female osmiophilic bodies prior to Plasmodium gametocyte egress from erythrocytes. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-01-22 Tomoko Ishino,Mayumi Tachibana,Minami Baba,Hideyuki Iriko,Takafumi Tsuboi,Motomi Torii
Plasmodium parasites cause malaria in mammalian hosts and are transmitted by Anopheles mosquitoes. Gametocytes, which differentiate from asexual-stage parasites, are activated by environmental changes when ingested into the mosquito midgut, and are rapidly released from erythrocytes prior to fertilization. Secretory proteins localized to osmiophilic bodies (OBs), organelles unique to gametocytes, have
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Translation initiation factors GleIF4E2 and GleIF4A can interact directly with the components of the pre-initiation complex to facilitate translation initiation in Giardia lamblia. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-01-20 Adxebanjo Najeem Adedoja,Timothy McMahan,John Patrick Neal,Siddhartha Hamal Dhakal,Seetharama Jois,Daniel Romo,Ken Hull,Srinivas Garlapati
Translation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5’ cap structure of the mRNA through eIF4E and facilitates the binding of the preinitiation complex (PIC) via protein-protein interactions of eIF4G with
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Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-01-17 Mohsen Arbabi,Hossein Hooshyar,Majid Lotfinia,Mohamad Ali Bakhshi
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Functional analysis of vasa/PL10-like genes in the ovary of Schistosoma mansoni. Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2020-01-17 Danielle E Skinner,Anastas Popratiloff,Yousef N Alrefaei,Victoria H Mann,Gabriel Rinaldi,Paul J Brindley
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Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host–parasite interaction and novel targets for diagnostics Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2019-12-24 Priscilla D.M. Fonseca, Marcelo A. Corral, Miguel Cosenza-Contreras, Dirce M.C.L. Meisel, Gessica B. Melo, Milena M.S. Antunes, Maria C.E. Santo, Ronaldo C.B. Gryschek, Julia M. Costa-Cruz, William Castro-Borges, Fabiana M. Paula
Strongyloides venezuelensis is an important alternative source of antigen for the serologic diagnosis of human strongyloidiasis. Proteomics techniques applied to the analysis of the protein content of infective third stage larvae (iL3) of S. venezuelensis provide a powerful tool for the discovery of new candidates for immunodiagnosis. This study presents an overview of the protein iL3 S. venezuelensis
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Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro Mol. Biochem. Parasitol. (IF 1.571) Pub Date : 2019-12-23 Jana Ilgová, Lenka Kavanová, Katarína Matiašková, Jiří Salát, Martin Kašný
The gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably compromise their hosts’ health status, fish with epithelial barriers damaged in parasite feeding and attachment sites are at an increased risk
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