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Ghrelin Affects Gastric Cancer Progression by Activating AMPK Signaling Pathway Biochem. Genet. (IF 2.027) Pub Date : 2021-01-13 Xiao-Lin Hu, Yong-Jun Zhu, Chang-Hua Hu, Li You, Juan Wu, Xiao-Yan He, Wen-Jie Huang, Zong-Hui Wu
As the endogenous ligand for the GH secretagogue receptor (GHSR), Ghrelin is aberrant expressed in multiple malignant carcinoma, and involved in regulating a number of progression of cancer, especially in metastasis and proliferation. However, the precise role of Ghrelin in tumorigenesis of gastric cancer (GC) is still poorly understood. In this study, we extensively investigated the roles and mechanisms
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Long Non-coding RNA FEZF1-AS1 Promotes Growth and Reduces Apoptosis Through Regulation of miR-363-3p/PAX6 Axis in Retinoblastoma Biochem. Genet. (IF 2.027) Pub Date : 2021-01-11 Xiuming Liu, Xiaofeng Li, Jianchang Li
Retinoblastoma is the most common malignancy in children's eyes with high incidence. Long non-coding RNAs (lncRNAs) play important roles in the progression of retinoblastoma. LncRNA FEZF1 antisense RNA 1 (FEZF1-AS1) has been found to stimulate retinoblastoma. However, the mechanism of FEZF1-AS1 underlying progression of retinoblastoma is still unclear. In current study, FEZF1-AS1 was up-regulated in
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miR-23b Attenuates LPS-Induced Inflammatory Responses in Acute Lung Injury via Inhibition of HDAC2 Biochem. Genet. (IF 2.027) Pub Date : 2021-01-07 Zhi-Feng Luo, Xiang-Hui Jiang, Huan Liu, Li-Yuan He, Xiong Luo, Fu-Chun Chen, Yu-Lin Tan
Inflammatory responses play significant role in infectious etiology-induced acute lung injury (ALI). Histone deacetylase 2 is found to be essential and stimulated in lipopolysaccharide (LPS)-induced ALI by regulating proinflammatory cytokines. miR-23b has been demonstrated to be downregulated in LPS-induced inflammatory injury. In this study, we aimed to explore the interaction between miR-23b and
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The Entire Mitochondrial Genome of Macrophthalmus abbreviatus Reveals Insights into the Phylogeny and Gene Rearrangements of Brachyura Biochem. Genet. (IF 2.027) Pub Date : 2021-01-07 Xinyi Xu, Qi Wang, Qiong Wu, Jiayan Xu, Jie Wang, Zhengfei Wang
Brachyuran crabs comprise the most species-rich clades among extant Decapoda and are divided into several major superfamilies. However, the phylogeny of Brachyuran remains controversial, comprehensive analysis of the overall phylogeny is still lacking. Complete mitochondrial genome (mitogenome) can indicate phylogenetic relationships, as well as useful information for gene rearrangement mechanisms
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Genome-Wide Identification of Copper Stress-Regulated and Novel MicroRNAs in Mulberry Leaf Biochem. Genet. (IF 2.027) Pub Date : 2021-01-03 Qiuxia Du, Peng Guo, Yisu Shi, Jian Zhang, Danyan Zheng, Yang Li, Adolf Acheampong, Ping Wu, Qiang Lin, Weiguo Zhao
Copper (Cu) is an essential trace element for plant growth and development. It is widely involved in respiration, photosynthesis, pollen formation, and other biological processes. Therefore, low or excessive copper causes damage to plants. Mulberry is an essential perennial economic tree. At present, research on the abiotic stress responses in mulberry is mainly focused on the identification of resistant
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LncRNA FTX Promotes Colorectal Cancer Cells Migration and Invasion by miRNA-590-5p/RBPJ Axis Biochem. Genet. (IF 2.027) Pub Date : 2021-01-03 Guo-Qun Chen, Zhi-Ming Liao, Jiao Liu, Fang Li, Da Huang, Ya-Dong Zhou
Colorectal cancer (CRC) is one of the most lethal human cancers all over the world. Moreover, it ranks fourth for cancer-related deaths among males. Although many efforts have been made to cure CRC, the effect remains limited. It has been reported that lncRNA five prime to Xist (FTX) was upregulated in CRC. However, the mechanism by which lncRNA FTX regulates the progression of CRC remains largely
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The KLF14 Variant is Associated with Type 2 Diabetes and HbA 1C Level Biochem. Genet. (IF 2.027) Pub Date : 2021-01-03 Ensieh Shahvazian, Mohammad Bagher Mahmoudi, Ehsan Farashahi Yazd, Saba Gharibi, Bahram Moghimi, Pouria HosseinNia, Masoud Mirzaei
The purpose of this study was to scan variants in coding region of Krȕppel like factor14 (KLF14) locus and assess association related to type 2 diabetes (T2D) in Iranian population. We sequenced the coding region of KLF14 to scan variants in case–sibling study (92 individuals with T2D and 92 healthy older siblings). To confirm, we analyzed rs76603546 association with T2D in a larger unrelated case–control
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Association of C677T (rs1081133) and A1298C (rs1801131) Methylenetetrahydrofolate Reductase Variants with Breast Cancer Susceptibility Among Asians: A Systematic Review and Meta-Analysis Biochem. Genet. (IF 2.027) Pub Date : 2021-01-02 Maryam Rezaee, Hamed Akbari, Mohammad Amin Momeni-Moghaddam, Fatemeh Moazzen, Sarvenaz Salahi, Reza Jahankhah, Sedigheh Tahmasebi
This systematic review and meta-analysis were conducted to investigate the association between methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms with breast cancer (BC) in Asians. Systematic searches were conducted in PubMed, EMBASE, Web of Science, and Scopus by May 2020. Inter-study heterogeneity was also assessed with a Q test, along with I2 statistics. Random-effects models
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Early Harvest Effects on Hydrophilic Phenolic Components of Extra Virgin Olive Oils Cvs. ‘Ayvalık’, ‘Memecik’ and ‘Topakaşı’ Biochem. Genet. (IF 2.027) Pub Date : 2020-11-23 Fatma Yıldırım, Adnan Nurhan Yıldırım, Gülcan Özkan, Bekir Şan, Mehmet Polat, Hatice Aşık, Yaşar Karakurt, Sezai Ercişli
The study was conducted to determine the effects of cultivar, harvest period and their interaction on the hydrophilic phenolic components extra virgin olive oils of the cultivars ‘Ayvalık’, ‘Memecik’ and ‘Topakaşı’. Olives were collected at three different harvesting periods; (1) early harvest period-1 (Beginning of spotting), (2) early harvest period-2 (End of spotting), and (3) optimum harvest period
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Lipoxin A4-Mediated p38 MAPK Signaling Pathway Protects Mice Against Collagen-Induced Arthritis Biochem. Genet. (IF 2.027) Pub Date : 2020-11-22 Jinyu Li, Qi Sun, Chenying Zheng, Chunxiao Bai, Chuyin Liu, Xueqian Zhao, Peiying Deng, Limin Chai, Yusong Jia
The aim of the article was to study the mechanism of Lipoxin A4 (LXA4)-mediated p38 MAPK pathway protecting mice against collagen-induced arthritis (CIA). The impact of LXA4 (0, 5, 10, 15 nM) on synoviocytes proliferation of CIA mice was detected using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. CIA mice were treated with LXA4, SB203580 (a p38 inhibitor), and/or anisomycin
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miR-617 Promotes the Growth of IL-22-Stimulated Keratinocytes Through Regulating FOXO4 Expression Biochem. Genet. (IF 2.027) Pub Date : 2020-11-19 Tao Liu, Xiaomei Feng, Yongmei Liao
Psoriasis is considered as a common chronic and relapsing inflammatory skin disease. MicroRNAs (miRNAs) were found to be related with psoriasis pathogenesis. Nevertheless, the function of miR-617 in psoriasis is still unclear. The miR-617 RNA level was detected using quantitative reverse transcription-PCR (qRT-PCR). Western blot analysis examined the protein level. Cell proliferation was analyzed via
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CYT387, a Novel JAK2 Inhibitor, Suppresses IL-13-Induced Epidermal Barrier Dysfunction Via miR-143 Targeting IL-13Rα1 and STAT3 Biochem. Genet. (IF 2.027) Pub Date : 2020-11-15 Yan Zu, Xiao-Fei Chen, Qiang Li, Shu-Ting Zhang
Atopic dermatitis (AD) is a chronic inflammatory skin disease influencing not only children but also adults. It is well-known that AD has a complex pathogenesis without effective therapy. Herein, we explored the function and mechanism of CYT387, a novel JAK2 inhibitor, on epidermal barrier damage. HaCaT cells exposed with high-concentration Ca2+ (1.8 mM) for 14 days were recruited for the model of
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AKR1C1 Contributes to Cervical Cancer Progression via Regulating TWIST1 Expression Biochem. Genet. (IF 2.027) Pub Date : 2020-11-10 Xing Wei, Zhongheng Wei, Yueyong Li, Zhongqiu Tan, Cheng Lin
Cervical cancer (CC) is a common gynecological malignancy, accounting for 10% of all gynecological cancers. Recently, targeted therapy for CC has shown unprecedented advantages. To improve CC patients’ prognosis, there are still urgent needs to develop more promising therapeutic targets. Aldo-keto reductase 1 family member C1 (AKR1C1) is a type of aldosterone reductase and plays a regulatory role in
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Vitamin D and Genetic Susceptibility to Multiple Sclerosis Biochem. Genet. (IF 2.027) Pub Date : 2020-11-07 Concetta Scazzone, Luisa Agnello, Giulia Bivona, Bruna Lo Sasso, Marcello Ciaccio
Multiple sclerosis (MS) is an autoimmune disease affecting the central nervous system (CNS), resulting from the interaction among genetic, epigenetic, and environmental factors. Vitamin D is a secosteroid, and its circulating levels are influenced by environment and genetics. In the last decades, research data on the association between MS and vitamin D status led to hypothesize a possible role for
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Associations of BCL2 CA-Repeat Polymorphism and Breast Cancer Susceptibility in Isfahan Province of Iran Biochem. Genet. (IF 2.027) Pub Date : 2020-11-05 Fatemeh Ghorbani, Seyed-Morteza Javadirad, Farzane Amirmahani, Zahra Fatehi, Manoochehr Tavassoli
BCL2 apoptosis regulator (BCL2) is a cause of tumorigenesis whose CA-repeat promoter polymorphisms has inconsistent association with various types of cancers. The association of BCL2 polymorphism with breast cancer was investigated in the Isfahan province of Iran. PCRamplification of the CA-repeat was followed by polyacrylamide gel electrophoresis and direct sequencing for 120 breast cancer women and
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Angiotensin-Converting Enzyme Gene Polymorphism Influences Gastrointestinal Motility in Type 2 Diabetes Mellitus Biochem. Genet. (IF 2.027) Pub Date : 2020-11-02 Aastha Malik, Sarama Saha, Rajesh Kumar Morya, Sanjay Kumar Bhadada, Praveen Kumar Singh, Satya Vati Rana
Uncontrolled diabetes mellitus may affect any part of the gastrointestinal tract (GIT) and impact negatively the quality of life. Angiotensin-converting enzyme (ACE) gene polymorphism can have direct effect on circulating level of ACE which further modifies the degradation of substance P and thus may influence the gut motility. Hence, it could be hypothesised that ACE gene polymorphism would influence
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Adaptive Evolution of Chalcone Isomerase Superfamily in Fagaceae Biochem. Genet. (IF 2.027) Pub Date : 2020-11-01 Li-Mei Lin, Hong-Yu Guo, Xin Song, Duo-Duo Zhang, Yue-Hong Long, Zhao-Bin Xing
Chalcone Isomerase (CHI) catalyzes the biosynthesis of flavonoids and secondary metabolism in plants. Currently, there is no systematic analysis of CHIs gene family in Fagaceae which is available. In this study, twenty-two CHI proteins were identified in five species of the Fagaceae family. The CHI superfamily in Fagaceae can be classified into three subfamilies and five groups using phylogenetic analysis
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Regulation of Apoptosis and Inflammatory Response in Interleukin-1β-Induced Nucleus Pulposus Cells by miR-125b-5p Via Targeting TRIAP1 Biochem. Genet. (IF 2.027) Pub Date : 2020-10-29 Jian Jie, Xiaoming Xu, Weilin Li, Guihua Wang
The aim of the present study was to determine the function of microRNA (miR)-125b-5p in lumbar disc degeneration (LDD). Nucleus pulposus (NP) cells were stimulated with 10 ng/ml IL-1β for 24 h to establish an LDD model. Reverse transcription-quantitative PCR was used to assess miR-125b-5p levels in human lumbar degenerative NP samples and IL-1β-treated NP cells. An interaction between miR-125b-5p and
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miR-491-3p is Downregulated in Retinoblastoma and Inhibit Tumor Cells Growth and Metastasis by Targeting SNN Biochem. Genet. (IF 2.027) Pub Date : 2020-10-23 Yang Hu, Ming Zhao, Li Li, Jie Ding, Yu-Min Gui, Tan-Wei Wei
Retinoblastoma (Rb) is the most common pediatric malignant tumor of the eyes. Previous studies demonstrated that miR-491-3p is downregulated in various cancers. However, its function in Rb remains unknown. A total of 15 pairs of primary Rb tissues and adjacent noncancerous tissues were collected. Quantitative real-time PCR (qRT-PCR) was used to investigate the expression profiles of miR-491-3p. qRT-PCR
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LncRNA XIST Promotes Migration and Invasion of Papillary Thyroid Cancer Cell by Modulating MiR-101-3p/CLDN1 Axis Biochem. Genet. (IF 2.027) Pub Date : 2020-10-14 Yong-Liang Du, Yan Liang, Yan Cao, Le Liu, Jian Li, Guang-Qing Shi
Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy in the worlds. Long non-coding RNA X-inactive specific transcript (XIST) was found to upregulate in PTC tissues and cell lines. However, the molecular mechanism underlying PTC metastasis and whether XIST plays regulatory role in PTC are still largely unknown. qRT-PCR was performed to detect the expression of lncRNA XIST and mRNAs
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A Novel Missense Single Nucleotide Polymorphism in the GREM1 Gene is Highly Associated with Higher Reproductive Traits in Awassi Sheep Biochem. Genet. (IF 2.027) Pub Date : 2020-10-13 Faris S. Imran, Tahreer M. Al-Thuwaini, Mohammed Baqur S. Al-Shuhaib, Frederic Lepretre
GREM1 (gremlin1) is a known inhibitor for BMP15 (bone morphogenetic protein 15) family, but its genetic diversity in sheep is unknown. The present study was conducted to analyze the polymorphism of GREM1 gene using PCR- single-strand conformation polymorphism (SSCP) and DNA sequencing methods and to assess the possible association of GREM1 gene polymorphism with reproductive traits in Awassi ewes.
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Biochemical Research of the Effects of Essential Oil Obtained from the Fruit of Myrtus communis L. on Cell Damage Associated with Lipopolysaccharide-Induced Endotoxemia in a Human Umbilical Cord Vein Endothelial Cells Biochem. Genet. (IF 2.027) Pub Date : 2020-10-12 Zerrin Kutlu, Mine Gulaboglu, Zekai Halıcı, İrfan Cınar, Busra Dıyarbakır
The aim of this study to investigate the potential effects of essential oils and compounds obtained from MC fruit on sepsis induced endothelial cell damage in human umbilical cord vein endothelial cells (HUVECs) at molecular and cellular levels on in vitro sepsis model. A sepsis model was induced by the application of LPS. The HUVEC treatment groups were as follows: control, LPS, MC, MC plus LPS, 1
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Leaf Transcriptome and Weight Gene Co-expression Network Analysis Uncovers Genes Associated with Photosynthetic Efficiency in Camellia oleifera Biochem. Genet. (IF 2.027) Pub Date : 2020-10-10 Zhilong He, Caixia Liu, Xiangnan Wang, Rui Wang, Yun Tian, Yongzhong Chen
Camellia oleifera Abel. (C. oleifera) as an important economic tree species in China has drawn growing attention because of its highly commercial, medic, cosmetic, and ornamental value. To deepen our understanding about the photosynthetic characters during the whole developmental stage as well as the molecular basis of photosynthesis, a comparative analysis of the leaf transcriptome of two C. oleifera
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TNF-α Induces URG-4/URGCP Gene Expression in Hepatoma Cells through Starvation Dependent Manner Biochem. Genet. (IF 2.027) Pub Date : 2020-10-09 Esra Tokay, Rahsan Ilikci Sagkan, Feray Kockar
URG-4/URGCP is a gene that may be associated with the onset of tumorigenesis and cell cycle regulation. In the literature, there is no study about inflammatory cytokine-mediated URG-4/URGCP regulation. In this study, the effect of TNF-α cytokine was investigated on URG-4/URGCP expression in serum-starved and serum-cultured hepatoma cells. The effect of TNF-α on hepatoma cells was shown using MTT and
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Genetic Diversity Assessment of a Chinese Medicinal Endemic Species, Aspidopterys obcordata var. obcordata , by Combined Molecular Marker Methods (ISSR & SRAP) Biochem. Genet. (IF 2.027) Pub Date : 2020-10-03 Mei-Fang Song, Yan-Hong Guan, Yue Zhang, Hai-Tao Li, Li-Xia Zhang, Zhong-Lian Zhang
Aspidopterys obcordata var. obcordata, a medicinal plant endemic to China, is a narrowly distributed species and wild resources are extremely limited. To evaluate the genetic variability and degree of genetic divergence of A. obcordata var. obcordata, and to make rational scientific decisions on its harvest and germplasm conservation, we collected 122 samples from across nearly all of its distribution
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Genome-Wide Investigation and Expression Analysis of K + -Transport-Related Gene Families in Chinese Cabbage ( Brassica rapa ssp. pekinensis ) Biochem. Genet. (IF 2.027) Pub Date : 2020-09-29 Changwei Shen, Jingping Yuan
Potassium (K+) transport and channel systems play vital roles in plant growth, development and responses to various stresses. In this study, 44 putative K+-transport-related genes (18K+ uptake permease (KUP)/high-affinity K+ (HAK)/K+ transporter (KT) family genes and 26 channel genes, including 18 Shaker family genes and 8K+ channel outward (KCO) family genes) were identified in the genome of Chinese
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A Crosstalk on Codon Usage in Genes Associated with Leukemia Biochem. Genet. (IF 2.027) Pub Date : 2020-09-28 Supriyo Chakraborty, Durbba Nath, Sunanda Paul, Yashmin Choudhury, Yeongseon Ahn, Yoon Shin Cho, Arif Uddin
Leukemia is the outcome of aggregation of damaged white blood cells. Several genes were reported to be associated with the pathogenesis of leukemia. These genes were computationally analyzed to decipher their codon usage bias (CUB) and to identify the prime factors influencing the codon usage profile as no work was reported yet. The mean values of synonymous codon usage order (SCUO) parameter indicated
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Population Genetics of Polyploid Complex Psidium cattleyanum Sabine (Myrtaceae): Preliminary Analyses Based on New Species-Specific Microsatellite Loci and Extension to Other Species of the Genus. Biochem. Genet. (IF 2.027) Pub Date : 2020-09-27 Raquel Moura Machado,Fernanda Ancelmo de Oliveira,Fábio de Matos Alves,Anete Pereira de Souza,Eliana Regina Forni-Martins
Polyploidy is a phenomenon that alters the genetic diversity of populations and has been reported as one of the most important evolutionary forces for plant diversification. The Psidium cattleyanum complex comprises a group of wild populations with several ploidy levels reported in the literature. The multiple cytotypes, associated with its wide distribution area, make this species a potential key
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Naringin Combined with NF-κB Inhibition and Endoplasmic Reticulum Stress Induces Apoptotic Cell Death via Oxidative Stress and the PERK/eIF2α/ATF4/CHOP Axis in HT29 Colon Cancer Cells. Biochem. Genet. (IF 2.027) Pub Date : 2020-09-26 Doğan Albayrak,Oğuzhan Doğanlar,Suat Erdoğan,Meryem Meraklı,Ayten Doğan,Pelin Turker,Ayten Bostancı,Zeynep Banu Doğanlar
Currently, combination therapy is considered the most effective solution for a selective chemotherapeutic effect in the treatment of colon cancer. This study investigated the death of both colon cancer HT29 cells and healthy vascular smooth muscle TG-Ha-VSMC cells (VSMCs) induced by naringin combined with endoplasmic reticulum (ER) stress and NF-κB inhibition. Naringin combined with tunicamycin and
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A Pooled Study of Angiotensin-Converting Enzyme Insertion/Deletion Gene Polymorphism in Relation to Risk, Pathology and Prognosis of Childhood Immunoglobulin A Vasculitis Nephritis. Biochem. Genet. (IF 2.027) Pub Date : 2020-09-26 Gao Hui,Zhang Cheng,Hua Ran,Wang Ziwei,Deng Fang
The angiotensin-converting enzyme (ACE) insertion/deletion (I/D) gene polymorphism has been inconsistently reported to be a risk factor for Childhood immunoglobulin A vasculitis (IgAV) nephritis. We comprehensively searched electronic databases as of Jan 2020. Nineteen studies with 1104 cases and 1589 controls were included. Sensitivity analyses based on different subgroups were performed. Further
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Gene Analysis of Genetically Modified Soybean Lectin Based on Fluorescence Quantitative PCR. Biochem. Genet. (IF 2.027) Pub Date : 2020-09-26 Zhaohui Wang,Hongyu Jia,Hansong Yu,Tong Wang,Bin Yang
Considering that the genetically modified soybean lectin gene is affected by the gene type, to improve the stability of the genetically modified soybean lectin gene, a method based on fluorescence quantitative PCR to analyze the genetic characteristics of the genetically modified soybean lectin was proposed. The common soybean varieties, Wangshuibai and Huangdou No. 3 were selected as materials for
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In Silico Analysis of HSP70 Gene Family in Bovine Genome. Biochem. Genet. (IF 2.027) Pub Date : 2020-08-25 Kabita Tripathy,Monika Sodhi,R S Kataria,Meenu Chopra,Manishi Mukesh
Heat shock proteins (HSPs), members of molecular chaperones families fulfill essential roles under normal conditions and provide protection and adaptation during and after stress. Among different HSPs, HSP70 kDa family of proteins is most abundant and well-studied in human and mouse but has not yet been characterized in bovines. In silico analysis was performed to characterize members of HSP70 gene
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Identification of a Growth-Associated Single Nucleotide Polymorphism (SNP) in Cyclin C of the Giant Tiger Shrimp Penaeus monodon. Biochem. Genet. (IF 2.027) Pub Date : 2020-08-11 Sirithorn Janpoom,Sirikan Prasertlux,Puttawan Rongmung,Piamsak Menasveta,Thanathip Lamkom,Panya Sae-Lim,Bavornlak Khamnamtong,Sirawut Klinbunga
The full-length cDNA of cyclin C of the giant tiger shrimp Penaeus monodon (PmCyC) was isolated by RACE-PCR. It was 1443 bp in length containing an open reading frame (ORF) of 804 bp and 267 deduced amino acids. Tissue distribution analysis indicated that PmCyC was more abundantly expressed in ovaries and testes than other tissues of female and male juveniles (P < 0.05). A pair of primers was designed
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Genome-Wide Investigation and Expression Profiling Under Abiotic Stresses of a Soybean Unknown Function (DUF21) and Cystathionine-β-Synthase (CBS) Domain-Containing Protein Family. Biochem. Genet. (IF 2.027) Pub Date : 2020-08-10 Qingnan Hao,Yanyan Yang,Zhihui Shan,Haifeng Chen,Chanjuan Zhang,Limiao Chen,Songli Yuan,Xiaojuan Zhang,Shuilian Chen,Zhonglu Yang,Dezhen Qiu,Xinan Zhou
Cystathionine-β-synthase (CBS) domain-containing proteins (CDCPs) constitute a large family in plants, and members of this family have been implicated in a variety of biological processes. However, the precise functions and the underlying mechanisms of most members of this family in plants remain to be elucidated. CBSDUF proteins belong to the CDCP superfamily, which contains one domain of unknown
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Polyprenol-Based Lipofecting Agents for In Vivo Delivery of Therapeutic DNA to Treat Hypertensive Rats. Biochem. Genet. (IF 2.027) Pub Date : 2020-08-06 Olga Gawrys,Monika Rak,Iwona Baranowska,Sylwia Bobis-Wozowicz,Karolina Szaro,Zbigniew Madeja,Ewa Swiezewska,Marek Masnyk,Marek Chmielewski,Elzbieta Karnas,Elzbieta Kompanowska-Jezierska
Development of efficient vectors for transfection is one of the major challenges in genetic engineering. Previous research demonstrated that cationic derivatives of polyisoprenoids (PTAI) may serve as carriers of nucleic acids. In the present study, the effectiveness of two PTAI-based formulations (PTAI-6–8 and 10–14) was investigated and compared to the commercial reagents. The purpose of applied
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Molecular and Evolutionary Characterization of Pollen S Determinant (SFB Alleles) in Four Diploid and Hexaploid Plum Species (Prunus spp.). Biochem. Genet. (IF 2.027) Pub Date : 2020-07-31 Donia Abdallah,Ghada Baraket,Sana Ben Mustapha,Marı A Angeles Moreno,Amel Salhi Hannachi
In more than 60 families of angiosperms, the self- and cross-fertilization is avoided through a complex widespread genetic system called self-incompatibility (SI). One of the major puzzling issues concerning the SI is the evolution of this system in species with complex polyploid genomes. Among plums, one of the first fruits species to attract human interest, polyploid species represent enormous genetic
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Association of ACE Gene Insertion/Deletion Polymorphism with Suicidal Attempt in an Iranian Population. Biochem. Genet. (IF 2.027) Pub Date : 2020-07-27 Elham Ghorbani,Mahsa Mohammadi,Seyed Kazem Malakouti,Hamed Mohammadi-Kangarani,Elaheh Abdollahi,Mansour Torab,Parvaneh Rahimi-Moghaddam
Deregulation of the renin–angiotensin system (RAS) plays an important role in suicide. Angiotensin converting enzyme (ACE) gene is a key component in this system. The relationship between insertion/deletion (I/D) polymorphism of ACE gene with suicide attempt (SA) is controversial. According to previous studies, allele D in this polymorphism has been considered as a potential risk factor for suicide
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Nuclear Factor-κB Pathway Mediates the Molecular Pathogenesis of LMNA-Related Muscular Dystrophies. Biochem. Genet. (IF 2.027) Pub Date : 2020-07-23 Yanbin Fan,Dandan Tan,Xu Zhang,Danyu Song,Xingzhi Chang,Shuang Wang,Hui Yan,Lin Ge,Haipo Yang,Carsten Bönnemann,Jingying Liu,Suxia Wang,Xiru Wu,Hong Zhang,Hui Xiong
LMNA-related muscular dystrophies are caused by mutations of the LMNA gene. Inflammatory changes and cellular apoptosis are significant pathological findings in the muscle cells of these patients. We aimed to investigate the roles of nuclear factor-κB (NF-κB) mediated inflammation as a molecular mechanism for the pathogenesis of LMNA-related muscular dystrophies. Muscle specimen of a patient with LMNA
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TIAM1 Upregulation Confers NVP-BEZ235 Resistance to Breast Cancer Cells Through FGFR/STAT3 Pathway. Biochem. Genet. (IF 2.027) Pub Date : 2020-07-15 Jingjing Qiao,Man Li,Na Li,Lingzhi Xu,Gena Huang,Jing Yu
Breast cancer is the most common cancer in women worldwide, and advanced breast cancer is the leading cause of cancer death in women. In present study, we aim to investigate that role of T-cell lymphoma invasion and metastasis-inducing protein1 (TIAM1) on NVP-BEZ235 resistance to breast cancer MCF7 and MDA-MB-361 cells. Briefly, MCF7 and MDA-MB-361 cells were treated with NVP-BEZ235 and the relative
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Correction To: Obesity, More than a 'Cosmetic' Problem. Current Knowledge and Future Prospects of Human Obesity Genetics. Biochem. Genet. (IF 2.027) Pub Date : 2020-07-13 Shabana,Shahida Hasnain
The authors regret that the reference provided to Fig. 1 of their publication was incorrect in the original publication. The authors obtained permission to reprint Fig. 1 retrospectively. The correct reference is: Nat Rev Genet. 2005 Mar;6(3):221–34. https://doi.org/10.1038/nrg1556.
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The rs2175898 Polymorphism in the ESR1 Gene has a Significant Sex-Specific Effect on Obesity. Biochem. Genet. (IF 2.027) Pub Date : 2020-07-10 Filiz Guclu-Geyik,Neslihan Coban,Gunay Can,Nihan Erginel-Unaltuna
Polymorphisms in the ESR1 gene have been associated with obesity and lipid metabolism. There are also important sex-related differences in the prevalence of obesity and related phenotypes. Therefore, we aimed to interrogate the association of the ESR1 rs2175898 gene polymorphism with obesity, obesity-related variables, and lipid levels in men and women as separate groups. Two thousand twenty-two randomly
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De Novo Assembly and Annotation of the Juvenile Tuber Transcriptome of a Gastrodia elata Hybrid by RNA Sequencing: Detection of SSR Markers. Biochem. Genet. (IF 2.027) Pub Date : 2020-07-06 Yunsheng Wang,Muhammad Qasim Shahid,Fozia Ghouri,Faheem Shehzad Baloch
Gastrodia elata is a traditional Chinese herbal medicine with good therapeutic effect on various nervous and cerebrovascular diseases. In the present study, we generated 20,611,556 raw reads from the young tuber transcriptome of a G. elata hybrid (Gastrodia elata BI.f.elata × Gastrodia elata BI.f.pilifera) by using Illumina HiSeq™ 4000 sequencing platform. De novo assembly and bioinformatics analysis
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miR-217/Mafb Axis Involve in High Glucose-Induced β-TC-tet Cell Damage Via Regulating NF-κB Signaling Pathway. Biochem. Genet. (IF 2.027) Pub Date : 2020-07-05 Huizhen Zheng,Xinying Li,Xinyu Yang,Fei Yan,Chuan Wang,Jinbo Liu
We attempt to explore the role of miR-217 during the process of type 2 diabetes mellitus (T2DM). Mouse β-TC-tet was dealt with 16.7 mM glucose (HG) to imitate the cells in T2DM. Cell proliferation and apoptosis were determined by cell counting kit-8 and flow cytometry. The correlation between miR-217 and Mafb was predicted with biological software and confirmed by dual lucifierase assay. Western blot
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The Effect of Sialic Acid on the Expression of miR-218, NF-kB, MMP-9, and TIMP-1. Biochem. Genet. (IF 2.027) Pub Date : 2020-06-30 Narjes Khatoun Shabani Sadr,Mohammad Shafiei,Hamid Galehdari,Alireza Khirolah
Sialic acid (N-acetylneuraminic acid, NANA) is found at all cell surfaces of vertebrates. Although it is widely accepted that sialic acid is an essential substrate for brain development via a significant role in nerve transfers, structure of glycosides, and synaptogenesis phenomena, there are some reports on the elevated levels of sialic acid and prevalence of neurodegeneration. Matrix metalloproteases
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LncRNA MIAT Promotes Inflammation and Oxidative Stress in Sepsis-Induced Cardiac Injury by Targeting miR-330-5p/TRAF6/NF-κB Axis. Biochem. Genet. (IF 2.027) Pub Date : 2020-06-15 Peng-Cheng Xing,Peng An,Guo-Yong Hu,Dong-Lian Wang,Min-Jie Zhou
Sepsis is a whole-body inflammation and main cause of death in intensive care units worldwide. We aimed to investigate the roles of lncRNA MIAT and miR-330-5p in modulating inflammatory responses and oxidative stress in lipopolysachariden (LPS)-induced septic cardiomyopathy. Serum and heart tissue were collected from in vivo septic mice model, ELISA and qRT-PCR were used to measure the expression of
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Genetic Polymorphisms Along with Dietary and Environmental Factors Enhance the Susceptibility to Nasopharyngeal Carcinoma in Nagaland of Northeast India. Biochem. Genet. (IF 2.027) Pub Date : 2020-06-18 Chenole Keppen,Prajjalendra Barooah,Parikhit Borthakur,Snigdha Saikia,Manab Deka,Sahana Bhattacharjee,Joshua Keppen,Vinotsole Khamo,Subhash Medhi
This study investigated the association of seven widely known DNA repair gene polymorphisms (hOGG1 Ser326Cys, XRCC1 Arg194Trp, XRCC1 Arg280His, XRCC1 Arg399Gln, XPC Val499Ala, XPD Lys751Gln and ERCC1 Cys8092Ala) with dietary and environmental factors for Nasopharyngeal Carcinoma (NPC) susceptibility in Nagaland of Northeast India. The genotypes were determined in 128 NPC patients and 180 healthy controls
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Long Non-coding RNA MIAT Mediates Non-small Cell Lung Cancer Development Through Regulating the miR-128-3p/PELI3 Axis. Biochem. Genet. (IF 2.027) Pub Date : 2020-06-16 Fannian Li,Haitao Li,Shuai Li,Baolei Lv,Junjie Shi,Hongjiang Yan,Helin Zhang,Yuzheng He
In this study, we set out to characterize the expression status of long non-coding RNA (lncRNA) Myocardial Infarction Associated Transcript (MIAT) in non-small cell lung cancer (NSCLC) and elucidate its mechanistic contribution to this disease. Relative expression levels of MIAT, Pellino E3 Ubiquitin Protein Ligase Family Member 3 (PELI3), and microRNA (miR)-128-3p were analyzed by real-time polymerase
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Genotype-Dependent Gene Expression in Strawberry (Fragaria x ananassa) Plants Under High Temperature Stress. Biochem. Genet. (IF 2.027) Pub Date : 2020-06-13 Müge Kesici,Ahmet Ipek,Figen Ersoy,Sergül Ergin,Hatice Gülen
The differences in tolerance to high temperatures were investigated on the basis of gene expressions in two strawberry (Fragaria x ananassa Duch) cultivars which were previously determined as high temperature tolerant (Redlands Hope = R. Hope) and sensitive (Festival). Plants were exposed incrementally to 35, 40, 45, and finally 50 °C for 24 h. qRT-PCR analyses were carried out with 19 known sequences
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Influence of Human Paraoxonase-1 Polymorphism (Q192R) on Serum Levels of Clinical Biomarkers Indicatives of Cardiovascular Diseases Risk in Mexican Women. Biochem. Genet. (IF 2.027) Pub Date : 2020-06-09 Ángeles C Ochoa-Martínez,Sandra T Orta-García,José A Varela-Silva,Iván N Pérez-Maldonado
Human paraoxonase-1 (PON1) is a hydrolytic enzyme related in the onset, establishment, and progression of inflammatory illnesses, including cardiovascular diseases (CVD). Therefore, the primary goal of this investigation was to determinate the influence of the PON1 gene polymorphism (Q192R) on prognostic biomarkers of CVD in women (n = 350) from San Luis Potosi, Mexico. For everyone enrolled, anthropometric
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Endothelial Nitric Oxide Synthase Gene Polymorphisms (- 922A > G, - 786 T > C, Intron 4 b/a VNTR and 894 G > T) and Essential Hypertension: An Association Study with Haplotypes Analysis. Biochem. Genet. (IF 2.027) Pub Date : 2020-06-09 Zahra Farbood,Amirsaeed Sabeti Aghabozorgi,Azim Nejatizadeh,Hossein Farshidi,Leila Shams,Amirhossein Bahreyni,Elahe Mansouri Babamansouri,Mohammad Shekari
Endothelial Nitric Oxide Synthase (eNOS) is an indispensable regulator of blood pressure through producing Nitric Oxide (NO). There is some evidence to suggest that eNOS gene polymorphisms are associated with Essential Hypertension (EHT). In this study, the potential association between eNOS 4a/4b, A922G, G894T, T786C gene polymorphisms and EHT as individual risk factors and as haplotypes are examined
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Identification and Distribution of NBS-Encoding Resistance Genes of Dactylis glomerata L. and Its Expression Under Abiotic and Biotic Stress. Biochem. Genet. (IF 2.027) Pub Date : 2020-06-06 Shuping Ren,Min Sun,Haidong Yan,Bingchao Wu,Tingting Jing,Linkai Huang,Bing Zeng
Orchardgrass (Dactylis glomerata L.) is drought resistant and tolerant to barren landscapes, making it one of the most important forages for animal husbandry, as well as ecological restoration of rocky landscapes that are undergoing desertification. However, orchardgrass is susceptible to rust, which can significantly reduce its yield and quality. Therefore, understanding the genes that underlie resistance
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Cytochrome P450 1A mRNA in the Gambusia affinis and Response to Several PAHs. Biochem. Genet. (IF 2.027) Pub Date : 2020-06-05 Shaolin Xie,Aiguo Zhou,Yongyong Feng,Yue Zhang,Junyi Li,Zhuolin Sun,Lanfen Fan,Jixing Zou
Cytochrome P4501A (CYP1A) has been used as a specific biomarker for monitoring water contamination such as PAHs, PCBs and dioxins. In the present study, the cyp1a gene of Gambusia affinis was cloned and sequenced and their expressions under PAHs exposure were characterized. The newly identified cyp1a encodes a protein with 521 amino acids that shared 96–80% identity with other Cyprinodontiformes fishes
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Unleash the Association of Mitochondrial Uncoupling Protein (UCP2) Promoter Variant (G-866A; rs659366) with Obesity: Stepping from a Case-Control Study to a Meta-analysis. Biochem. Genet. (IF 2.027) Pub Date : 2020-05-30 Hamada A Abd El Daim,Afaf M Elsaid,Amany A Mousa,Mervat M El-Eshmawy,Lashin S Lashin,Eman A Toraih,Rami M Elshazli
Numerous eligible articles investigated the potential impact of the promoter region of UCP2 (rs659366) variant and the susceptibility for obesity with questionable outcomes. Our team designed this case–control combined with meta-analysis survey to illustrate the contribution of this variant with obesity. This case–control survey was formulated based on 110 obese Egyptian patients and 122 non-obese
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MELK Accelerates the Progression of Colorectal Cancer via Activating the FAK/Src Pathway. Biochem. Genet. (IF 2.027) Pub Date : 2020-05-29 Guangming Liu,Wei Zhan,Wei Guo,Feng Hu,Junjie Qin,Rui Li,Xin Liao
Maternal embryo leucine zipper kinase (MELK) has a higher expression level in a variety of cancers and involved in progression of colorectal cancer. The MELK expression levels in colorectal cancer tissues and cells were detected by RT-qPCR. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and transwell assays were used to examine the effect of the MELK konckdown on the proliferation
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Suppression of miR-30a-3p Attenuates Hepatic Steatosis in Non-alcoholic Fatty Liver Disease. Biochem. Genet. (IF 2.027) Pub Date : 2020-05-17 De-Run Wang,Bing Wang,Ming Yang,Zhen-Lu Liu,Jing Sun,Yan Wang,Hui Sun,Liang-Jun Xie
Non-alcoholic fatty liver disease (NAFLD) have a high prevalence in humans in the past two decades. Here, we elucidated the functions of miR-30a-3p in the development of NAFLD and identified its potential targets. HepG-2 cells and NAFLD patients' blood samples were used in our study. Bioinformatics analysis as well as luciferase reporter assays were employed to distinguish peroxisome proliferator-activated
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Genome-Wide Identification and Analysis of the Growth-Regulating Factor (GRF) Gene Family and GRF-Interacting Factor Family in Triticum aestivum L. Biochem. Genet. (IF 2.027) Pub Date : 2020-05-12 Ting Zan,Li Zhang,Tingting Xie,Liqun Li
Growth-regulating factors (GRFs) are unique transcription factors in plants. GRFs can interact with SNH (SYT N-terminal homology) domains in GRF-interacting factor (GIF) proteins via the N-terminal QLQ (Gln, Leu, Gln) domain to form functional complexes and participate in the regulation of downstream gene expression. In this study, we systematically identified the GRF gene family and GIF gene family
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High Resolution Melting (HRM) Genotyping in the Genus Origanum: Molecular Identification and Discrimination for Authentication Purposes. Biochem. Genet. (IF 2.027) Pub Date : 2020-05-11 Charikleia Papaioannou,Konstantina Zeliou,Panayiotis Trigas,Vasileios Papasotiropoulos
Origanum L. (Lamiaceae) is an important genus of medicinal and aromatic plants used since ancient times as culinary herbs and remedies in traditional medicine. Although it is a relatively small genus, intra-generic species delineation, as well as its inter-generic relationships within tribe Mentheae, are still poorly understood. High resolution melting (HRM) analysis, coupled with microsatellite markers
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Comparison of mRNA Expression of P2X Receptor Subtypes in Different Arterial Tissues of Rats. Biochem. Genet. (IF 2.027) Pub Date : 2020-05-08 Ling-Zhi Li,Li-Hui Yue,Zhi-Min Zhang,Jing Zhao,Lei-Ming Ren,Hong-Jie Wang,Lu Li
This study aims to compare the expression of P2X receptor subtype mRNA in different arterial tissues of rats. After the rats were sacrificed, the internal carotid, pulmonary, thoracic aorta, mesenteric and caudal arteries were dissected out. Then, the P2X receptor mRNA expression in different blood vessels was detected by reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative
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Length Polymorphisms in the Angiotensin I-Converting Enzyme Gene and the Serotonin-Transporter-Linked Polymorphic Region Constitute a Risk Haplotype for Depression in Patients with Coronary Artery Disease. Biochem. Genet. (IF 2.027) Pub Date : 2020-05-05 Thomas Meyer,Isabel Rothe,Julia Staab,Hans-Christian Deter,Stella V Fangauf,Stefanie Hamacher,Martin Hellmich,Jana Jünger,Karl-Heinz Ladwig,Matthias Michal,Katja Petrowski,Joram Ronel,Wolfgang Söllner,Cora Weber,Martina de Zwaan,Redford B Williams,Christian Albus,Christoph Herrmann-Lingen,
Genetic variations affecting the course of depressive symptoms in patients with coronary artery disease (CAD) have not yet been well studied. Therefore, we set out to investigate whether distinct haplotypes of the two insertion/deletion polymorphisms in the serotonin-transporter-linked polymorphic region (5-HTTLPR) and the angiotensin I-converting enzyme (ACE) gene located on chromosome 17 can be identified
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Descending Expression of miR320 in Insulin-Resistant Adipocytes Treated with Ascending Concentrations of Metformin. Biochem. Genet. (IF 2.027) Pub Date : 2020-05-04 Yousof Naghiaee,Reza Didehdar,Zahra Malekpour-Dehkordi,Fatemeh Pourrajab,Javad Mohiti-Ardakani
Some miRNAs are supposed to play a role in insulin resistance and metabolic disorders. Such miRNAs can be differentially expressed in response to a pharmacologic intervention for insulin resistance as a biomarker/risk factor for insulin resistance. This study aimed at determining the effect of Metformin on miR320 expression in insulin-resistant (IR) adipocytes. The 3T3L1 cells were expanded in DMEM
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