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Glycans modulate lipid binding in Lili-Mip lipocalin protein: insights from molecular simulations and protein network analyses. Glycobiology (IF 4.3) Pub Date : 2023-11-28 Harini SureshKumar,Rajeswari Appadurai,Anand Srivastava
The unique viviparous Pacific Beetle cockroaches provide nutrition to their embryo by secreting milk proteins Lili-Mip, a lipid-binding glycoprotein that crystallises in-vivo. The resolved in-vivo crystal structure of variably glycosylated Lili-Mip shows a classical Lipocalin fold with an eight-stranded antiparallel beta-barrel enclosing a fatty acid. The availability of physiologically unaltered glycoprotein
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Knockout of the intellectual disability-linked gene Hs6st2 in mice decreases heparan sulfate 6-O-sulfation, impairs dendritic spines of hippocampal neurons, and affects memory. Glycobiology (IF 4.3) Pub Date : 2023-11-28 Sohyun Moon,Hiu Ham Lee,Stephanie Archer-Hartmann,Naoko Nagai,Zainab Mubasher,Mahima Parappurath,Laiba Ahmed,Raddy L Ramos,Koji Kimata,Parastoo Azadi,Weikang Cai,Jerry Yingtao Zhao
Heparan sulfate (HS) is a linear polysaccharide that plays a key role in cellular signaling networks. HS functions are regulated by its 6-O-sulfation, which is catalyzed by three HS 6-O-sulfotransferases (HS6STs). Notably, HS6ST2 is mainly expressed in the brain and HS6ST2 mutations are linked to brain disorders, but the underlying mechanisms remain poorly understood. To determine the role of Hs6st2
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Developing a solid-phase method for the enzymatic synthesis of heparan sulfate and chondroitin sulfate backbones. Glycobiology (IF 4.3) Pub Date : 2023-11-23 Eduardo Stancanelli,Wei Liu,Guowei Su,Vijay Padagala,Jian Liu
Despite the recent progress on the solution-phase enzymatic synthesis of heparan sulfate (HS) and chondroitin sulfate (CS), solid-phase enzymatic synthesis has not been fully investigated. Here, we describe the solid-phase enzymatic synthesis of HS and CS backbone oligosaccharides using specialized linkers. We demonstrate the use of immobilized HS linker to synthesize CS, and the use of immobilized
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Reusable glycan microarrays using a microwave assisted wet-erase (MAWE) process. Glycobiology (IF 4.3) Pub Date : 2023-11-14 Akul Y Mehta,Catherine A Tilton,Lukas Muerner,Stephan Gunten,Jamie Heimburg-Molinaro,Richard D Cummings
Modern studies on binding of proteins to glycans commonly involve the use of synthetic glycans and their derivatives in which a small amount of the material is covalently printed onto a functionalized slide in a glycan microarray format. While incredibly useful to explore binding interactions with many types of samples, the common techniques involve drying the slides, which leads to irreversible association
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Glycosyltransferases as versatile tools to study the biology of glycans. Glycobiology (IF 4.3) Pub Date : 2023-11-13 Joshua M Kofsky,Jonathan L Babulic,Marie E Boddington,Fabiola V De León González,Chantelle J Capicciotti
All cells are decorated with complex carbohydrate structures called glycans that serve as ligands for glycan-binding proteins (GBPs) to mediate a wide range of biological processes. Understanding the specific functions of glycans is key to advancing an understanding of human health and disease. However, the lack of convenient and accessible tools to study glycan-based interactions has been a defining
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Lewis a-b- histo-blood group antigen phenotype is predictive of severe COVID-19 in the black South African population group. Glycobiology (IF 4.3) Pub Date : 2023-11-09 Cliff A Magwira,Ndivho P Nndwamato,Gloria Selabe,Mapaseka L Seheri
Several risk factors have been associated with SARS-CoV-2 infections and severity of COVID-19 disease it causes. This study investigated whether variations in histo-blood group antigen (HBGA) expression can predispose individuals to SARS-CoV-2 infections and severity of the disease. Nasopharyngeal swabs, randomly selected from SARS-CoV-2 positive and SARS-CoV-2 negative individuals, were tested for
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Deglycosylated RBD produced in Pichia pastoris as a low-cost sera COVID-19 diagnosis tool and a vaccine candidate. Glycobiology (IF 4.3) Pub Date : 2023-11-07 Tommy Idrovo-Hidalgo,María F Pignataro,Luis M Bredeston,Fernanda Elias,María G Herrera,María F Pavan,Sabrina Foscaldi,Mayra Suireszcz,Natalia B Fernández,Diana E Wetzler,Carlos H Paván,Patricio O Craig,Ernesto A Roman,Lucas A M Ruberto,Diego G Noseda,Lorena I Ibañez,Cecilia Czibener,,Juan E Ugalde,Alejandro D Nadra,Javier Santos,Cecilia D'Alessio
During the COVID-19 outbreak, numerous tools including protein-based vaccines have been developed. The methylotrophic yeast Pichia pastoris (synonymous to Komagataella phaffii) is an eukaryotic cost-effective and scalable system for recombinant protein production, with the advantages of an efficient secretion system and the protein folding assistance of the secretory pathway of eukaryotic cells. In
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GM1 ganglioside protects against LPS-induced neuroinflammatory and oxidative responses by inhibiting the activation of Akt, TAK1 and NADPH oxidase in MG6 microglial cells. Glycobiology (IF 4.3) Pub Date : 2023-11-03 Ting-Yin Yeh,Wen-Jui Chu,Yuahn-Sieh Huang
GM1 is a major brain ganglioside that exerts neurotrophic, neuroprotective and antineuroinflammatory effects. The aim of this study was to obtain insights into the antineuroinflammatory mechanisms of exogenous GM1 in lipopolysaccharide (LPS)-stimulated MG6 mouse transformed microglial cell line. First, we found that GM1 prevented the LPS-induced transformation of microglia into an amoeboid-like shape
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Corrigendum to: Mucosal glycan degradation of the host by the gut microbiota. Glycobiology (IF 4.3) Pub Date : 2023-10-30
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Src is a target molecule of mannose against pancreatic cancer cells growth in vitro & in vivo. Glycobiology (IF 4.3) Pub Date : 2023-10-30 Jianhao Xie,Shengjie Wu,Wenfeng Liao,Jingru Ning,Kan Ding
Pancreatic ductal adenocarcinoma (PDAC) is a highly malignant cancer with limited treatment options. Mannose, a common monosaccharide taken up by cells through the same transporters as glucose, has been shown to induce growth retardation and enhance cell death in response to chemotherapy in several cancers, including PDAC. However, the molecular targets and mechanisms underlying mannose's action against
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Cholera intoxication of human enteroids reveals interplay between decoy and functional glycoconjugate ligands. Glycobiology (IF 4.3) Pub Date : 2023-10-30 Akshi Singla,Andrew Boucher,Kerri-Lee Wallom,Michael Lebens,Jennifer J Kohler,Frances M Platt,Ulf Yrlid
Prior research on cholera toxin (CT) binding and intoxication has relied on human colonic cancer derived epithelial cells. While these transformed cell lines have been beneficial, they neither derive from small intestine where intoxication occurs, nor represent the diversity of small intestinal epithelial cells (SI-ECs) and variation in glycoconjugate expression among individuals. Here, we used human
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Biochemical characterization of a new ulvan lyase and its applicability in utilization of ulvan and preparation of ulva oligosaccharides. Glycobiology (IF 4.3) Pub Date : 2023-10-30 Chen Li,Tiancheng Tang,Jinju Jiang,Zhong Yao,Benwei Zhu
Ulva is globally distributed specie and has a high economic value. Ulvan is one of the main active substances in Ulva, which has a variety of biological properties. Ulvan lyase degrades ulvan through a β-elimination mechanism which cleaves the β-glycosidic bond between Rha3S and GlcA or IdoA. The complex monosaccharide composition of ulvan makes it promising for use in food and pharmaceutical applications
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Polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) isozyme surface charge governs charge substrate preferences to modulate mucin type O-glycosylation. Glycobiology (IF 4.3) Pub Date : 2023-10-30 Collin J Ballard,Miya R Paserba,Earnest James Paul Daniel,Ramón Hurtado-Guerrero,Thomas A Gerken
A large family of polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) initiate mucin type O-glycosylation transferring α-GalNAc from a UDP-GalNAc donor to the hydroxyl groups of Ser and Thr residues of peptides and proteins, thereby defining sites of O-glycosylation. Mutations and differential expression of several GalNAc-Ts are associated with many disease states including cancers. The mechanisms
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A photoaffinity glycan-labeling approach to investigate immunoglobulin glycan-binding partners. Glycobiology (IF 4.3) Pub Date : 2023-10-29 Miles D Holborough-Kerkvliet,Greta Mucignato,Sam J Moons,Venetia Psomiadou,Rohit S R Konada,Nichole J Pedowitz,Matthew R Pratt,Theresa Kissel,Carolien A M Koeleman,Rayman T N Tjokrodirijo,Petrus A van Veelen,Thomas Huizinga,Karin A J van Schie,Manfred Wuhrer,Jennifer J Kohler,Kimberly M Bonger,Thomas J Boltje,Reinaldus E M Toes
Glycans play a pivotal role in biology. However, because of the low-affinity of glycan-protein interactions, many interaction pairs remain unknown. Two important glycoproteins involved in B-cell biology are the B-cell receptor and its secreted counterpart, antibodies. It has been indicated that glycans expressed by these B-cell-specific molecules can modulate immune activation via glycan-binding proteins
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Targeting aberrant sialylation and fucosylation in prostate cancer cells using potent metabolic inhibitors. Glycobiology (IF 4.3) Pub Date : 2023-10-17 Margarita Orozco-Moreno,Eline A Visser,Kirsty Hodgson,Agnes L Hipgrave Ederveen,Kayla Bastian,Emily Archer Goode,Özden Öztürk,Johan F A Pijnenborg,Nienke Eerden,Sam J Moons,Emiel Rossing,Ning Wang,Noortje Haan,Christian Büll,Thomas J Boltje,Jennifer Munkley
Aberrant glycosylation is a hallmark of cancer and is not just a consequence, but also a driver of a malignant phenotype. In prostate cancer, changes in fucosylated and sialylated glycans are common and this has important implications for tumour progression, metastasis, and immune evasion. Glycans hold huge translational potential and new therapies targeting tumour-associated glycans are currently
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Tn antigen interactions of macrophage galactose-type lectin (MGL) in immune function and disease. Glycobiology (IF 4.3) Pub Date : 2023-10-17 Berna Tumoglu,Aidan Keelaghan,Fikri Y Avci
Protein-carbohydrate interactions are essential in maintaining immune homeostasis and orchestrating inflammatory and regulatory immune processes. This review elucidates the immune interactions of macrophage galactose-type lectin (MGL, CD301) and Tn carbohydrate antigen. MGL is a C-type lectin receptor (CLR) primarily expressed by myeloid cells such as macrophages and immature dendritic cells. MGL recognizes
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Elucidation of bacterial trehalose-degrading trehalase and trehalose phosphorylase: physiological significance and its potential applications. Glycobiology (IF 4.3) Pub Date : 2023-10-17 Prasansah Shrestha,Jayram Karmacharya,So-Ra Han,Jun Hyuck Lee,Tae-Jin Oh
Bacteria possess diverse metabolic and genetic processes, resulting in the inability of certain bacteria to degrade trehalose. However, some bacteria do have the capability to degrade trehalose, utilizing it as a carbon source, and for defense against environmental stress. Trehalose, a disaccharide, serves as a carbon source for many bacteria, including some that are vital for pathogens. The degradation
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Galectin-3 does not interact with RNA directly. Glycobiology (IF 4.3) Pub Date : 2023-10-10 Egan L Peltan,Nicholas M Riley,Ryan A Flynn,David S Roberts,Carolyn R Bertozzi
Galectin-3, well characterized as a glycan binding protein, has been identified as a putative RNA binding protein, possibly through participation in pre-mRNA maturation through interactions with splicosomes. Given recent developments with cell surface RNA biology, the putative dual-function nature of galectin-3 evokes a possible non-classical connection between glycobiology and RNA biology. However
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The intersection of HIF-1α, O-GlcNAc, and skeletal muscle loss in chronic obstructive pulmonary disease. Glycobiology (IF 4.3) Pub Date : 2023-10-09 Jinendiran Sekar,Amy Attaway
Sarcopenia, defined as the loss of muscle mass and strength, is a major cause of morbidity and mortality in COPD (chronic obstructive pulmonary disease) patients. However, the molecular mechanisms that cause sarcopenia remain to be determined. In this review, we will highlight the unique molecular and metabolic perturbations that occur in the skeletal muscle of COPD patients in response to hypoxia
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Galectin-12 modulates Kupffer cell polarization to alter the progression of nonalcoholic fatty liver disease. Glycobiology (IF 4.3) Pub Date : 2023-10-06 Jyun-Lin Lee,Yao-Chien Wang,Yu-An Hsu,Chih-Sheng Chen,Rui-Cian Weng,Yen-Pei Lu,Chun-Yu Chuang,Lei Wan
Nonalcoholic fatty liver disease is caused by an imbalance in lipid metabolism and immune response to pose a risk factor for liver fibrosis. Recent evidence indicates that M2 macrophages secrete transforming growth factor-β1, which contributes to liver fibrosis. Galectin-12 has been demonstrated to regulate lipid metabolism and macrophage polarization. The purpose of this study is to investigate the
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Identification of potential classes of glycoligands mediating dynamic endothelial adhesion of human tumor cells. Glycobiology (IF 4.3) Pub Date : 2023-10-06 Sarah Starzonek,Hanna Maar,Stefan Mereiter,Vera Freytag,Marie-Therese Haider,Kristoffer Riecken,Yen-Lin Huang,Francis Jacob,Daniel Wicklein,Udo Schumacher,Tobias Lange
One critical step of metastasis formation is the extravasation of circulating tumor cells from the bloodstream. This process requires the dynamic interaction of cell adhesion molecules like E-selectin on endothelial cells with carbohydrate ligands on tumor cells. To characterize these glycans in a comprehensible approach, the rolling, tethering, and firm adhesion of nine human tumor cell lines on human
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Macrophage N-glycan processing inhibits antibody-dependent cellular phagocytosis. Glycobiology (IF 4.3) Pub Date : 2023-10-04 Jesús Aguilar Díaz de León,Isaac Aguilar,Adam W Barb
Factors regulating macrophage effector function represent potential targets to optimize the efficacy of antibody-mediated therapies. Macrophages are myeloid cells capable of engulfing and destroying diseased or damaged target cells. Antibodies binding to the target cell surface can engage macrophage Fc gamma receptors (FcγRs) to elicit antibody-dependent cellular phagocytosis (ADCP), a process that
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N-glycomic profiling of capsid proteins from adeno-associated virus serotypes. Glycobiology (IF 4.3) Pub Date : 2023-09-29 Yongjing Xie,Michael Butler
Adeno-associated virus (AAV) vector has become the leading platform for gene delivery. Each serotype exhibits a different tissue tropism, immunogenicity, and in vivo transduction performance. Therefore, selecting the most suitable AAV serotype is critical for efficient gene delivery to target cells or tissues. Genome divergence among different serotypes is due mainly to the hypervariable regions of
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Hyaluronan synthases; mechanisms, myths, & mysteries of three types of unique bifunctional glycosyltransferases. Glycobiology (IF 4.3) Pub Date : 2023-09-28 Paul L DeAngelis,Jochen Zimmer
Hyaluronan (HA), the essential [-3-GlcNAc-1-β-4-GlcA-1-β-]n matrix polysaccharide in vertebrates and molecular camouflage coating in select pathogens, is polymerized by "HA synthase" (HAS) enzymes. The first HAS identified three decades ago opened the window for new insights and biotechnological tools. This review discusses current understanding of HA biosynthesis, its biotechnological utility, and
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The role of the glycome in symbiotic host-microbe interactions. Glycobiology (IF 4.3) Pub Date : 2023-09-23 Rustam Aminov,Leila Aminova
Glycosylation plays a crucial role in many aspects of cell biology, including cellular and organismal integrity, structure-and-function of many glycosylated molecules in the cell, signal transduction, development, cancer, and in a number of diseases. Besides, at the inter-organismal level of interaction, a variety of glycosylated molecules are involved in the host-microbiota recognition and initiation
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Protein N-glycosylation in the bronchoalveolar space differs between never-smokers and long-term smokers with and without COPD. Glycobiology (IF 4.3) Pub Date : 2023-09-01 Vignesh Venkatakrishnan,Kristina A Thomsson,Médea Padra,Anders Andersson,Bettina Brundin,Karin Christenson,Johan Bylund,Niclas G Karlsson,Anders Lindén,Sara K Lindén
Chronic obstructive pulmonary disease (COPD) kills millions of people annually and patients suffering from exacerbations of this disorder display high morbidity and mortality. The clinical course of COPD is associated with dysbiosis and infections, but the underlying mechanisms are poorly understood. Glycosylation of proteins play roles in regulating interactions between microbes and immune cells,
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A single-step, rapid and versatile method for simultaneous detection of cell surface glycan profiles using fluorochrome-conjugated lectins. Glycobiology (IF 4.3) Pub Date : 2023-08-16 Nicolás I Torres,Montana N Manselle Cocco,Ramiro M Perrota,Yamil D Mahmoud,Mariana Salatino,Karina V Mariño,Gabriel A Rabinovich
Cell surface glycans play essential roles in diverse physiological and pathological processes and their assessment has important implications in biomedicine and biotechnology. Here we present a rapid, versatile and single-step multicolor flow cytometry method for evaluation of cell surface glycan signatures using a panel of selected fluorochrome-conjugated lectins. This procedure allows simultaneous
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Protein O-mannosylation: one sugar, several pathways, many functions. Glycobiology (IF 4.3) Pub Date : 2023-08-11 Melissa Koff,Pedro Monagas-Valentin,Boris Novikov,Ishita Chandel,Vladislav Panin
Recent research has unveiled numerous important functions of protein glycosylation in development, homeostasis, and diseases. A type of glycosylation taking center stage is protein O-mannosylation (POM), a posttranslational modification conserved in a wide range of organisms, from yeast to humans. In animals, POM plays a crucial role in the nervous system, while POM defects cause severe neurological
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Quantification of orally administered chondroitin sulfate oligosaccharides in human plasma and urine. Glycobiology (IF 4.3) Pub Date : 2023-10-29 Hiroko Mizuta,Shota Kawahara,Naonobu Tsutsumi,Nobuyuki Miyamoto
Chondroitin sulfate has been widely administered orally to improve knee osteoarthritis. Chondroitin sulfate also has various biological properties, such as anti-inflammatory, immunomodulatory, anti-oxidative, and antitumor activity. However, chondroitin sulfate absorption in the digestive system and bioavailability remains controversial owing to its large molecular weight. In this study, we aimed to
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Expression of low-sulfated keratan sulfate in non-mucinous ovarian carcinoma. Glycobiology (IF 4.3) Pub Date : 2023-07-13 Hitomi Hoshino,Ya-Ying Chen,Daisuke Inoue,Yoshio Yoshida,Kay-Hooi Khoo,Tomoya O Akama,Motohiro Kobayashi
Keratan sulfate glycosaminoglycan is composed of repeating N-acetyllactosamine (LacNAc) disaccharide units consisting of galactose (Gal) and N-acetylglucosamine (GlcNAc), both often 6-O-sulfated. Sulfate contents of keratan sulfate are heterogeneous depending upon the origins. In this study, keratan sulfate is classified as either highly sulfated (in which both GlcNAc and Gal residues are 6-O-sulfated)
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Structural and mechanistic studies of the N-glycosylation machinery: From lipid-linked oligosaccharide biosynthesis to glycan transfer. Glycobiology (IF 4.3) Pub Date : 2023-07-03 Ana S Ramírez,Kaspar P Locher
N-linked protein glycosylation is a post-translational modification that exists in all domains of life. It involves two consecutive steps: (1) biosynthesis of a lipid-linked oligosaccharide (LLO), and (2) glycan transfer from the LLO to asparagine residues in secretory proteins, which is catalyzed by the integral membrane enzyme oligosaccharyltransferase (OST). In the last decade, structural and functional
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Role of the ST6GAL1 sialyltransferase in regulating ovarian cancer cell metabolism. Glycobiology (IF 4.3) Pub Date : 2023-10-06 Robert B Jones,Austin D Silva,Katherine E Ankenbauer,Colleen M Britain,Asmi Chakraborty,Jamelle A Brown,Scott W Ballinger,Susan L Bellis
The ST6GAL1 sialyltransferase, which adds α2-6-linked sialic acids to N-glycosylated proteins, is upregulated in many malignancies including ovarian cancer. Through its activity in sialylating select surface receptors, ST6GAL1 modulates intracellular signaling to regulate tumor cell phenotype. ST6GAL1 has previously been shown to act as a survival factor that protects cancer cells from cytotoxic stressors
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VSIG4 interaction with heparan sulfates inhibits VSIG4-complement binding. Glycobiology (IF 4.3) Pub Date : 2023-08-14 Sarah Y Ebstein,Ashique Rafique,Yi Zhou,Amanda Krasco,Welby Montalvo-Ortiz,Lola Yu,Luisaidy Custodio,Rene C Adam,Nicolin Bloch,Ken Lee,Funmilola Adewale,Dominic Vergata,Antonio Luz,Sebastien Coquery,Benjamin Daniel,Erica Ullman,Matthew C Franklin,Aynur Hermann,Tammy Huang,William Olson,Samuel Davis,Andrew J Murphy,Matthew A Sleeman,Joyce Wei,Dimitris Skokos
V-set and immunoglobulin domain-containing 4 (VSIG4) is a complement receptor of the immunoglobulin superfamily that is specifically expressed on tissue resident macrophages, and its many reported functions and binding partners suggest a complex role in immune function. VSIG4 is reported to have a role in immune surveillance as well as in modulating diverse disease phenotypes such as infections, autoimmune
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Conformational comparisons of Pasteurella multocida types B and E and structurally related capsular polysaccharides. Glycobiology (IF 4.3) Pub Date : 2023-10-29 Nicole I Richardson,Neil Ravenscroft,Michelle M Kuttel
Pasteurella multocida, an encapsulated gram-negative bacterium, is a significant veterinary pathogen. The P. multocida is classified into 5 serogroups (A, B, D, E, and F) based on the bacterial capsular polysaccharide (CPS), which is important for virulence. Serogroups B and E are the primary causative agents of bovine hemorrhagic septicemia that is associated with significant yearly losses of livestock
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Two NOTCH1 O-fucose sites have opposing functions in mouse retinal angiogenesis. Glycobiology (IF 4.3) Pub Date : 2023-10-06 Rachel K LoPilato,Heike Kroeger,Sneha K Mohan,James D Lauderdale,Neil Grimsey,Robert S Haltiwanger
Previous in vitro studies demonstrated that Fringe glycosylation of the NOTCH1 extracellular domain at O-fucose residues in Epidermal Growth Factor-like Repeats (EGFs) 6 and 8 is a significant contributor to suppression of NOTCH1 activation by JAG1 or enhancement of NOTCH1 activation by DLL1, respectively. In this study, we sought to evaluate the significance of these glycosylation sites in a mammalian
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Supplementing the Carbohydrate Structure Database with glycoepitopes. Glycobiology (IF 4.3) Pub Date : 2023-08-14 Philip V Toukach
Carbohydrate structures in the Carbohydrate Structure Database have been referenced to glycoepitopes from the Immune Epitope Database allowing users to explore the glycan structures and contained epitopes. Starting with an epitope, one can figure out the glycans from other organisms that share the same structural determinant, and retrieve the associated taxonomical, medical, and other data. This database
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Direct antitumoral effects of sulfated fucans isolated from echinoderms: a possible role of neuropilin-1/β1 integrin endocytosis and focal adhesion kinase degradation. Glycobiology (IF 4.3) Pub Date : 2023-10-29 Antonio G F Lima,Viviane W Mignone,Francisco Vardiero,Eliene O Kozlowski,Laila R Fernandes,Juliana M Motta,Mauro S G Pavão,Camila C Figueiredo,Paulo A S Mourão,Verônica Morandi
Hypercoagulability, a major complication of metastatic cancers, has usually been treated with heparins from natural sources, or with their synthetic derivatives, which are under intense investigation in clinical oncology. However, the use of heparin has been challenging for patients with risk of severe bleeding. While the systemic administration of heparins, in preclinical models, has shown primarily
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Single-mutations at the galactose-binding site of enzymes GalK, GalU, and LgtC enable the efficient synthesis of UDP-6-azido-6-deoxy-d-galactose and azido-functionalized Gb3 analogs. Glycobiology (IF 4.3) Pub Date : 2023-10-06 Maria E Ortiz-Soto,Makarius Baier,Daniela Brenner,Malte Timm,Jürgen Seibel
Lysosomal accumulation of the glycosphingolipid globotriaosylceramide Gb3 is linked to the deficient activity of the α-galactosidase A in the Anderson-Fabry disease and an elevated level of deacylated Gb3 is a hallmark of this condition. Localization of Gb3 in the plasma membrane is critical for studying how the membrane organization and its dynamics are affected in this genetic disorder. Gb3 analogs
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Role of glycosylation in breast cancer progression and metastasis: implications for miRNA, EMT and multidrug resistance. Glycobiology (IF 4.3) Pub Date : 2023-08-14 Rohitesh Gupta,Rohan Ponangi,Kuppanur G Indresh
Breast cancer (BC) is one of the leading causes of death in women, globally. A variety of biological processes results in metastasis, a poorly understood pathological phenomenon, causing a high relapse rate. Glycosylation, microribonucleic acids (miRNAs) and epithelial to mesenchymal transition (EMT), have been shown to regulate this cascade where tumor cells detach from their primary site, enter the
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Targeting altered glycosylation in secreted tumor glycoproteins for broad cancer detection. Glycobiology (IF 4.3) Pub Date : 2023-08-14 Matthew R Kudelka,Wei Gu,Yasuyuki Matsumoto,Tongzhong Ju,Richard H Barnes Ii,Robert J Kardish,Jamie Heimburg-Molinaro,Sylvain Lehoux,Junwei Zeng,Cynthia Cohen,Brian S Robinson,Kinjal S Shah,Elliot L Chaikof,Sean R Stowell,Richard D Cummings
There is an urgent need to develop new tumor biomarkers for early cancer detection, but the variability of tumor-derived antigens has been a limitation. Here we demonstrate a novel anti-Tn antibody microarray platform to detect Tn+ glycoproteins, a near universal antigen in carcinoma-derived glycoproteins, for broad detection of cancer. The platform uses a specific recombinant IgG1 to the Tn antigen
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The potential role of nondigestible Raffinose family oligosaccharides as prebiotics. Glycobiology (IF 4.3) Pub Date : 2023-05-17 Freeha Kanwal,Dingxin Ren,Wajiha Kanwal,Mengying Ding,Junqing Su,Xiaoya Shang
Based on factual scientific health claims, prebiotics have gained significant importance in ever-growing food and pharmaceutical industries. The diverse nature of distinct prebiotics influences the host differently in distinguishable patterns. Functional oligosaccharides are either plant-derived or commercially prepared. Raffinose, stachyose, and verbascose are the 3 types of raffinose family oligosaccharides
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rBC2LCN-reactive SERPINA3 is a glycobiomarker candidate for pancreatic ductal adenocarcinoma. Glycobiology (IF 4.3) Pub Date : 2023-05-17 Shuuji Mawaribuchi,Osamu Shimomura,Tatsuya Oda,Keiko Hiemori,Kayoko Shimizu,Kenya Yamase,Mutsuhiro Date,Hiroaki Tateno
Early detection is urgently needed to improve the patient's pancreatic ductal adenocarcinoma (PDAC) survival. Previously, we identified a novel tumor-associated glycan, H-type3, which is expressed on PDAC cells and is detected by rBC2LCN (recombinant N-terminal domain of BC2L-C identified from Burkholderia cenocepacia) lectin. Here, we identified that SERPINA3 is an rBC2LCN-reactive glycoprotein (BC2-S3)
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Revised structure model of norovirus-binding fucoidan from Undaria pinnatifida: oligofucose chains branch off from a β6-galactane. Glycobiology (IF 4.3) Pub Date : 2023-08-14 Franz-Georg Hanisch
Fucoidans are discussed as antiviral agents, and fucoidan from Undaria pinnatifida (UpF), in particular has gained interest as potential food additive in antinoroviral strategies. As the competitive blocking activity of antinoroviral agents increases with the valency of terminal nonreducing fucose on the competitor, an effective processing of fucoidans to inhibitory oligosaccharides will depend on
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Computational studies on glycosaminoglycan recognition of sialyl transferases. Glycobiology (IF 4.3) Pub Date : 2023-08-14 Nehru Viji Sankaranarayanan,Srinivas Sistla,Balaji Nagarajan,John E Chittum,Joseph T Y Lau,Umesh R Desai
Despite decades of research, glycosaminoglycans (GAGs) have not been known to interact with sialyl transferases (STs). Using our in-house combinatorial virtual library screening (CVLS) technology, we studied seven human isoforms, including ST6GAL1, ST6GAL2, ST3GAL1, ST3GAL3, ST3GAL4, ST3GAL5, and ST3GAL6, and predicted that GAGs, especially heparan sulfate (HS), are likely to differentially bind to
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Siglec cis-ligands and their roles in the immune system. Glycobiology (IF 4.3) Pub Date : 2023-08-14 Takeshi Tsubata
Sialic acid-binding immunoglobulin-like lectins are a family of membrane molecules primarily expressed in immune cells. Most of them are inhibitory receptors containing immunoreceptor tyrosine-based inhibition motifs in the cytoplasmic tail. On the cell surface, sialic acid-binding immunoglobulin-like lectins are mostly bound by sialylated glycans on membrane molecules expressed in the same cell (cis-ligands)
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Siglec15 promotes the migration of thyroid carcinoma cells by enhancing the EGFR protein stability. Glycobiology (IF 4.3) Pub Date : 2023-06-21 Sijing Huang,Zhi Ji,Jinqiang Xu,Yuerong Yang,BingRui Wu,Qihang Chen,Shuting Geng,Yu Si,Jiayue Chen,Yuanyan Wei,Cong Wang,Zhilong Ai,Jianhai Jiang
PURPOSE Sialic acid-bound immunoglobulin-like lectin 15 (Siglec15) has emerged as a novel therapeutic target in tumor immunotherapy. This study is designed to investigate the function and mechanism of Siglec15 in thyroid carcinoma (THCA). MATERIALS AND METHODS The information on patients with THCA from TGCA and GEO database were used to analyze the expression of Siglec15 in THCA. THCA cells were treated
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Bridging glycoinformatics and cheminformatics: integration efforts between GlyCosmos and PubChem. Glycobiology (IF 4.3) Pub Date : 2023-06-21 Tiejun Cheng,Tamiko Ono,Masaaki Shiota,Issaku Yamada,Kiyoko F Aoki-Kinoshita,Evan E Bolton
The GlyCosmos Glycoscience Portal (https://glycosmos.org) and PubChem (https://pubchem.ncbi.nlm.nih.gov/) are major portals for glycoscience and chemistry, respectively. GlyCosmos is a portal for glycan-related repositories, including GlyTouCan, GlycoPOST, and UniCarb-DR, as well as for glycan-related data resources that have been integrated from a variety of 'omics databases. Glycogenes, glycoproteins
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Dynamic expression of mucins and the genes controlling mucin-type O-glycosylation within the mouse respiratory system. Glycobiology (IF 4.3) Pub Date : 2023-06-21 E Tian,Zulfeqhar A Syed,Matthew L Edin,Darryl C Zeldin,Kelly G Ten Hagen
The COVID-19 global pandemic has underscored the need to understand how viruses and other pathogens are able to infect and replicate within the respiratory system. Recent studies have highlighted the role of highly O-glycosylated mucins in the protection of the respiratory system as well as how mucin-type O-glycosylation may be able to modify viral infectivity. Therefore, we set out to identify the
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BiotechLec: an interactive guide of commercial lectins for glycobiology and biomedical research applications. Glycobiology (IF 4.3) Pub Date : 2023-10-29 Boris Schnider,Francisco L Escudero,Anne Imberty,Frédérique Lisacek
For decades, lectins have been used as probes in glycobiology and this usage has gradually spread to other domains of Life Science. Nowadays, researchers investigate glycan recognition with lectins in diverse biotechnology and clinical applications, addressing key questions regarding binding specificity. The latter is documented in scattered and heterogeneous sources, and this situation calls for a
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Correction: Glycan diversity in the course of vertebrate evolution. Glycobiology (IF 4.3) Pub Date : 2023-06-21
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Neuraminidase 4 (NEU4): new biological and physiological player. Glycobiology (IF 4.3) Pub Date : 2023-04-19 Sarah Okun,Allyson Peek,Suleiman A Igdoura
Sialidases are found in viruses, bacteria, fungi, avians, and mammals. Mammalian sialidases differ in their specificity, optimum pH, subcellular localization, and tissue expression. To date, four genes encoding mammalian sialidases (NEU1-4) have been cloned. This review examines the functional impact of NEU4 sialidase on complex physiological and cellular processes. The intracellular localization and
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Novel inhibitory effect of galectin-3 on the respiratory burst induced by Staphylococcus aureus in human neutrophils. Glycobiology (IF 4.3) Pub Date : 2023-06-21 Vignesh Venkatakrishnan,Jonas Elmwall,Trisha Lahiri,Martina Sundqvist,Linda Bergqvist,Hakon Leffler,Ulf J Nilsson,Amanda Welin,Johan Bylund,Anna Karlsson-Bengtsson
Among the responders to microbial invasion, neutrophils represent the earliest and perhaps the most important immune cells that contribute to host defense with the primary role to kill invading microbes using a plethora of stored anti-microbial molecules. One such process is the production of reactive oxygen species (ROS) by the neutrophil enzyme complex NADPH-oxidase, which can be assembled and active
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LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model. Glycobiology (IF 4.3) Pub Date : 2023-06-03 Subash C Pakhrin,Suresh Pokharel,Kiyoko F Aoki-Kinoshita,Moriah R Beck,Tarun K Dam,Doina Caragea,Dukka B Kc
Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes. It occurs at the N-X-[S/T] sequon in amino acid sequences, where X can be any amino acid except proline. However, not all N-X-[S/T] sequons are glycosylated; thus, the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation
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A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers. Glycobiology (IF 4.3) Pub Date : 2023-06-03 Julius Benicky,Miloslav Sanda,Aswini Panigrahi,Jian Liu,Zhangjie Wang,Vijayakanth Pagadala,Guowei Su,Radoslav Goldman
Sulf-2 is an extracellular heparan 6-O-endosulfatase involved in the postsynthetic editing of heparan sulfate (HS), which regulates many important biological processes. The activity of the Sulf-2 and its substrate specificity remain insufficiently characterized in spite of more than two decades of studies of this enzyme. This is due, in part, to the difficulties in the production and isolation of this
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The sweet side of sex as a biological variable. Glycobiology (IF 4.3) Pub Date : 2023-06-03 Carmanah D Hunter,Kaylee M Morris,Tahlia Derksen,Lisa M Willis
Glycobiology as a field holds enormous potential for understanding human health and disease. However, few glycobiology studies adequately address the issue of sex differences in biology, which severely limits the conclusions that can be drawn. Numerous CAZymes, lectins, and other carbohydrate-associated molecules have the potential to be differentially expressed and regulated with sex, leading to differences
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Substrate specificity and transglycosylation capacity of α-L-fucosidases across GH29 assessed by bioinformatics-assisted selection of functional diversity. Glycobiology (IF 4.3) Pub Date : 2023-06-03 Valentina N Perna,Kristian Barrett,Anne S Meyer,Birgitte Zeuner
Glycoside hydrolase family 29 (GH29) encompasses α-L-fucosidases, i.e. enzymes that catalyze the hydrolytic release of fucose from fucosylated glycans, including N- and O-linked glycans on proteins, and these α-L-fucosidases clearly play important roles in biology. GH29 enzymes work via a retaining exo-action mechanism, and some can catalyze transfucosylation. There is no formal subfamily division
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A lectin from Crenomytilus grayanus as a probe for the detection of widespread cancerous and metastatic cells. Glycobiology (IF 4.3) Pub Date : 2023-06-03 Yan-Fen Jhou,Feng-Ling Yang,Kuo-Shiang Liao,Chung-Yi Wu,I-Ming Lee
A novel Gal-binding lectin from mussels (Crenomytilus grayanus, CGL) with 6 binding sites in the dimeric structure has been previously shown to have antifungal, anticancer, and antibacterial activities. In this study, a glycan array was used to confirm that CGL recognizes a range of non-reducing end α- or β-linked Gal glycans on normal cells but not sialic acid-capped glycans. This finding suggests
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Correction to: Characterizing the selectivity of ER α-glucosidase inhibitors. Glycobiology (IF 4.3) Pub Date : 2023-06-21
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Toward an experimental system for the examination of protein mannosylation in Actinobacteria. Glycobiology (IF 4.3) Pub Date : 2023-06-21 Hirak Saxena,Nakita Buenbrazo,Won-Yong Song,Connie Li,Denis Brochu,Anna Robotham,Wen Ding,Luc Tessier,Rui Chen,John Kelly,Warren Wakarchuk
The Actinobacterial species Cellulomonas fimi ATCC484 has long been known to secrete mannose-containing proteins, but a closer examination of glycoproteins associated with the cell has never been reported. Using ConA lectin chromatography and mass spectrometry, we have surveyed the cell-associated glycoproteome from C. fimi and collected detailed information on the glycosylation sites of 19 cell-associated
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Impact of hyaluronan size on localization and solubility of the extracellular matrix in the mouse brain. Glycobiology (IF 4.3) Pub Date : 2023-10-06 Diana Egorova,Yoshihiro Nomura,Shinji Miyata
Hyaluronan (HA) is a central component of the extracellular matrix (ECM) in the brain and plays a pivotal role in neural development and plasticity. Brain HA exists in 2 distinct forms of the ECM: the diffuse ECM, which is soluble in saline and detergents, and the condensed ECM, which forms aggregates, such as perineuronal nets (PNNs). Although the physiological functions of HA significantly differ