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Turning the spotlight on the oligosaccharide chain of GM1 ganglioside Glycoconj. J. (IF 2.197) Pub Date : 2021-02-23 Elena Chiricozzi, Erika Di Biase, Giulia Lunghi, Maria Fazzari, Nicoletta Loberto, Massimo Aureli, Laura Mauri, Sandro Sonnino
It is well over a century that glycosphingolipids are matter of interest in different fields of research. The hydrophilic oligosaccharide and the lipid moiety, the ceramide, both or separately have been considered in different moments as the crucial portion of the molecule, responsible for the role played by the glycosphingolipids associated to the plasma-membranes or to any other subcellular fraction
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Up‐regulation of FUT8 inhibits TGF-β1-induced activation of hepatic stellate cells during liver fibrogenesis Glycoconj. J. (IF 2.197) Pub Date : 2021-02-20 Mengzhen Kuang, Hao Wu, Lan Hu, Xinying Guo, Daochuan He, Bo Liu, Mengqian Chen, Jie Gu, Jianxin Gu, Xiaoqing Zeng, Yuanyuan Ruan
Liver fibrosis is a continuous wound healing response caused by chronic liver injury, and the activation of hepatic stellate cells (HSCs) is considered as the main event for it. Core fucosylation catalyzed by FUT8 refers to adding the fucosyl moiety to the innermost GlcNAc residue of N-linked oligosaccharides and is involved in many biological processes such as cell differentiation, migration, and
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Aberrant RL2 O -GlcNAc antibody reactivity against serum-IgA1 of patients with colorectal cancer Glycoconj. J. (IF 2.197) Pub Date : 2021-02-20 Chris Verathamjamras, Tanin-ek Sriwitool, Pukkavadee Netsirisawan, Parunya Chaiyawat, Daranee Chokchaichamnankit, Naiyarat Prasongsook, Chantragan Srisomsap, Jisnuson Svasti, Voraratt Champattanachai
O-GlcNAcylation, a single attachment of N-acetylglucosamine (GlcNAc) on serine and threonine residues, plays important roles in normal and pathobiological states of many diseases. Aberrant expression of O-GlcNAc modification was found in many types of cancer including colorectal cancer (CRC). This modification mainly occurs in nuclear-cytoplasmic proteins; however, it can exist in some extracellular
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Variations in the mRNA expression level of UDP-GlcNAc epimerase/ManNAc kinase and neuraminidase 1 genes in organs of type 2 diabetic animals Glycoconj. J. (IF 2.197) Pub Date : 2021-02-20 Mayen David Inim, Mohammed Auwal Ibrahim, Murtala Bindawa Isah, Elewechi Onyike
Sialic acid and its associated metabolic enzymes have emerged as important components of the pathophysiology of type 2 diabetes (T2D). There is an elevation in the serum concentration of sialic acid in humans and animals with T2D. The present study investigated the modulation of mRNA expression level of UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) and neuraminidase 1 (NEU1)
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Identification of potential glycoprotein biomarkers in oral squamous cell carcinoma using sweet strategies Glycoconj. J. (IF 2.197) Pub Date : 2021-02-06 Yin-Ling Wong, Ramanathan Anand, Kar Mun Yuen, Wan Mahadzir Wan Mustafa, Mannil Thomas Abraham, Keng Kiong Tay, Zainal Ariff Abdul Rahman, Yeng Chen
The prevalence of oral squamous cell carcinoma (OSCC) is high in South and Southeast Asia regions. Most OSCC patients are detected at advanced stages low 5-year survival rates. Aberrant expression of glycosylated proteins was found to be associated with malignant transformation and cancer progression. Hence, identification of cancer-associated glycoproteins could be used as potential biomarkers that
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Identification of fucosylated haptoglobin‐producing cells in pancreatic cancer tissue and its molecular mechanism Glycoconj. J. (IF 2.197) Pub Date : 2021-02-01 Nami Ito, Momoko Yamada, Koichi Morishita, Satoshi Nojima, Kei Motooka, Natsumi Sakata, Tatsuya Asuka, Ryoji Otsu, Shinji Takamatsu, Yoshihiro Kamada, Soichiro Mori, Hirofumi Akita, Hidetoshi Eguchi, Eiichi Morii, Eiji Miyoshi
Fucosylated haptoglobin is a well-established glyco-biomarker of pancreatic cancer. We recently established a novel anti-glycan antibody (10-7G mAb) that specifically recognizes fucosylated haptoglobins, including prohaptoglobin (proHpt). Serum concentrations of the 10-7G value, as measured by ELISA, were increased in patients with pancreatic cancer relative to the healthy controls. However, it is
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A novel acid polysaccharide from Boletus edulis : extraction, characteristics and antitumor activities in vitro Glycoconj. J. (IF 2.197) Pub Date : 2021-01-28 Ting Meng, Sha-sha Yu, Hai-yu Ji, Xiao-meng Xu, An-jun Liu
A novel cold-water-soluble polysaccharide (BEP), with a molecular weight of 6.0 × 106 Da, was isolated from Boletus edulis. BEP consists of galactose, glucose, xylose, mannose, glucuronic, and galacturonic acid in a ratio of 0.34:0.28:0.28:2.57:1.00:0.44. The IR results showed that BEP was an acid polysaccharide, containing α-type and β-type glucoside bonds. MTT assay showed BEP could inhibit cell
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The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions Glycoconj. J. (IF 2.197) Pub Date : 2021-01-18 Tamas Pongracz, Aswin Verhoeven, Manfred Wuhrer, Noortje de Haan
Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles. Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers. Typically, during the first step of such derivatization reactions
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40 years of glyco-polyacrylamide in glycobiology Glycoconj. J. (IF 2.197) Pub Date : 2021-01-14 Alexander Tuzikov, Alexander Chinarev, Nadezhda Shilova, Elena Gordeeva, Oxana Galanina, Tatyana Ovchinnikova, Marcel Schaefer, Nicolai Bovin
Polyacrylamide conjugates of glycans have long been widely used in many research areas of glycobiology, mainly for immobilizing glycans in solid-phase assays and as multivalent inhibitors. Pending biotin tag allows immobilizing Glyc-PAA quantitatively on any surface, and acts as a tracer for detection of carbohydrate-binding proteins. However, the scope of already realized capabilities of these probes
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Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform Glycoconj. J. (IF 2.197) Pub Date : 2021-01-13 Asaduzzaman Md, Megumi Maeda, Teruaki Matsui, Yoshihiro Takasato, Komei Ito, Yoshinobu Kimura
Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds. The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified. In this study, we found a new variant of Ara
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Neuroprotective effect of heparin Trisulfated disaccharide on ischemic stroke Glycoconj. J. (IF 2.197) Pub Date : 2021-01-07 Gabrielly M. D. Chiarantin, Lina M. Delgado-Garcia, Laura N. Zamproni, Marcelo A. Lima, Helena B. Nader, Ivarne L. S. Tersariol, Marimélia Porcionatto
Cells undergoing hypoxia experience intense cytoplasmic calcium (Ca2+) overload. High concentrations of intracellular calcium ([Ca2+]i) can trigger cell death in the neural tissue, a hallmark of stroke. Neural Ca2+ homeostasis involves regulation by the Na+/Ca2+ exchanger (NCX). Previous data published by our group showed that a product of the enzymatic depolymerization of heparin by heparinase, the
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Recent advances in understanding the roles of sialyltransferases in tumor angiogenesis and metastasis Glycoconj. J. (IF 2.197) Pub Date : 2021-01-07 Chunyan Xu, Shidan Wang, Yinshuang Wu, Xiaoxin Sun, Deyong Yang, Shujing Wang
Abnormal glycosylation is a common characteristic of cancer cells and there is a lot of evidence that glycans can regulate the biological behavior of tumor cells. Sialylation modification, a form of glycosylation modification, plays an important role in cell recognition, cell adhesion and cell signal transduction. Abnormal sialylation on the surface of tumor cells is related to tumor migration and
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Structural analysis of glycosaminoglycans from Oviductus ranae Glycoconj. J. (IF 2.197) Pub Date : 2021-01-07 Haiyue Huang, Jin Mao, Quntao Liang, Jianghui Lin, Lilong Jiang, Shutao Liu, Joshua S. Sharp, Zheng Wei
Oviductus ranae (O.ran.) has been widely used as a tonic and a traditional animal-based Chinese medicine. O.ran. extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic. Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals
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Metal cations promote α-dicarbonyl formation in glucose-containing peritoneal dialysis fluids Glycoconj. J. (IF 2.197) Pub Date : 2020-12-07 Sabrina Gensberger-Reigl, Andrea Auditore, Jochen Huppert, Monika Pischetsrieder
Heat sterilization of peritoneal dialysis fluids (PDFs) leads to the formation of glucose degradation products (GDPs), which impair long-term peritoneal dialysis. The current study investigated the effects of metal ions, which occur as trace impurities in the fluids, on the formation of six major α-dicarbonyl GDPs, namely glucosone, glyoxal, methylglyoxal, 3-deoxyglucosone, 3-deoxygalactosone, and
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Glycation-mediated protein crosslinking and stiffening in mouse lenses are inhibited by carboxitin in vitro Glycoconj. J. (IF 2.197) Pub Date : 2020-11-27 Sandip K. Nandi, Johanna Rankenberg, Stefan Rakete, Rooban B. Nahomi, Marcus A. Glomb, Mikhail D. Linetsky, Ram H. Nagaraj
Proteins in the eye lens have negligible turnover and therefore progressively accumulate chemical modifications during aging. Carbonyls and oxidative stresses, which are intricately linked to one another, predominantly drive such modifications. Oxidative stress leads to the loss of glutathione (GSH) and ascorbate degradation; this in turn leads to the formation of highly reactive dicarbonyl compounds
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Development of a conventional immunochemical detection system for determination of N δ -(5-hydro-5-methyl-4-imidazolone-2-yl)-ornithine in methylglyoxal-modified proteins Glycoconj. J. (IF 2.197) Pub Date : 2020-11-25 Hiroko Yamaguchi, Mime Nagai, Hikari Sugawa, Hisataka Yasuda, Ryoji Nagai
Methylglyoxal (MGO) produced during glycolysis is known to react with arginine residues on proteins to generate advanced glycation end products, such as Nδ-(5-hydro-5-methyl-4-imidazolone-2-yl)-ornithine (MG-H1). Since the production of MGO is increased during hyperglycemia or metabolic disorders in vivo, it is considered that the measurement of MG-H1 is useful for evaluating abnormalities in carbohydrate
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SELECT-GLYCOCIN: a recombinant microbial system for expression and high-throughput screening of glycocins Glycoconj. J. (IF 2.197) Pub Date : 2020-11-18 Pravinkumar Choudhary, Alka Rao
Glycosylated bacteriocins (glycocins) are potential clean label food preservatives and new alternatives to antibiotics. Further development requires the availability of a method for laboratory evolution of glycocins, wherein the challenges to overcome include ensuring glycosylation in a heterologous host, avoiding potential toxicity of active glycocins to the host, and provisioning of a one-pot screening
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Modulation of calcium signaling depends on the oligosaccharide of GM1 in Neuro2a mouse neuroblastoma cells Glycoconj. J. (IF 2.197) Pub Date : 2020-11-17 Giulia Lunghi, Maria Fazzari, Erika Di Biase, Laura Mauri, Sandro Sonnino, Elena Chiricozzi
Recently, we demonstrated that the oligosaccharide portion of ganglioside GM1 is responsible, via direct interaction and activation of the TrkA pathway, for the ability of GM1 to promote neuritogenesis and to confer neuroprotection in Neuro2a mouse neuroblastoma cells. Recalling the knowledge that ganglioside GM1 modulates calcium channels activity, thus regulating the cytosolic calcium concentration
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New approach to prepare fluorogenic branched dextrins for assaying glycogen debranching enzyme Glycoconj. J. (IF 2.197) Pub Date : 2020-11-17 Miyu Sakaguchi, Yasushi Makino, Hiroshi Matsubara
Glycogen debranching enzyme (GDE), together with glycogen phosphorylase (GP), is responsible for the complete degradation of glycogen. GDE has distinct catalytic sites for 4-α-glucanotransferase and amylo-α-1,6-glucosidase. For the GDE sensitive assay, we previously developed the GP limit fluorogenic branched dextrin Glcα1–4Glcα1–4Glcα1–4Glcα1–4(Glcα1–4Glcα1–4Glcα1–4Glcα1–6)Glcα1–4Glcα1–4Glcα1–4GlcPA
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Analysis of serum glycome by lectin microarrays for prostate cancer patients - a search for aberrant glycoforms Glycoconj. J. (IF 2.197) Pub Date : 2020-10-29 Tomas Bertok, Eduard Jane, Nikola Chrenekova, Stefania Hroncekova, Aniko Bertokova, Michal Hires, Alica Vikartovska, Petra Kubanikova, Roman Sokol, Juraj Fillo, Peter Kasak, Lubor Borsig, Jan Tkac
This is the first work focused on glycoprofiling of whole N- and O- glycome using lectins in an array format applied for analysis of serum samples from healthy individuals, benign prostate hyperplasia (BPH) patients, and prostate cancer (PCa) patients. Lectin microarray was prepared using traditional lectins with the incorporation of 2 recombinant bacterial lectins and 3 human lectins (17 lectins in
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Glycation reaction and the role of the receptor for advanced glycation end-products in immunity and social behavior Glycoconj. J. (IF 2.197) Pub Date : 2020-10-27 Nontaphat Leerach, Ai Harashima, Seiichi Munesue, Kumi Kimura, Yu Oshima, Hisanori Goto, Hiroshi Yamamoto, Haruhiro Higashida, Yasuhiko Yamamoto
The receptor for advanced glycation end-products (receptor for AGEs, RAGE) is a pattern recognition receptor. The interaction of RAGE with its ligands, such as AGEs, S100 proteins, high mobility group box-1 (HMGB1), and lipopolysaccharides (LPS), is known to play a pivotal role in the propagation of immune responses and inflammatory reactions. The ligand-RAGE interaction elicits cellular responses
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Regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene expression during differentiation of human osteoblastic MG-63 cells Glycoconj. J. (IF 2.197) Pub Date : 2020-10-27 So-Young An, Miri Lee, Hyun-Kyoung Yoon, Fukushi Abekura, Kyoung-Sook Kim, Dong-Hyun Kim, Hyeon-Jun Kim, Kichoon Lee, Cheorl-Ho Kim, Young-Choon Lee
In this study, we found that gene expression of the human β-galactoside α2,6-sialyltransferase (hST6Gal I) was specifically increased during differentiation of human MG-63 osteoblastic cells by serum starvation (SS). In parallel, a distinct increase in binding to SNA, the α2,6-sialyl-specific lectin, was observed in serum-starved cells, as demonstrated by FACS analysis. 5’-Rapid amplification of cDNA
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Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment Glycoconj. J. (IF 2.197) Pub Date : 2020-10-17 Shuang Yang, Yan Wang, Matthew Mann, Qiong Wang, E Tian, Liping Zhang, John F. Cipollo, Kelly G. Ten Hagen, Lawrence A. Tabak
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A novel glycosidase plate-based assay for the quantification of galactosylation and sialylation on human IgG Glycoconj. J. (IF 2.197) Pub Date : 2020-10-16 Osmond D. Rebello, Richard A. Gardner, Paulina A. Urbanowicz, David N. Bolam, Lucy I. Crouch, David Falck, Daniel I. R. Spencer
Changes in human IgG galactosylation and sialylation have been associated with several inflammatory diseases which are a major burden on the health care system. A large body of work on well-established glycomic and glycopeptidomic assays has repeatedly demonstrated inflammation-induced changes in IgG glycosylation. However, these assays are usually based on specialized analytical instrumentation which
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A chronic strain of the cystic fibrosis pathogen Pandoraea pulmonicola expresses a heterogenous hypo-acylated lipid A Glycoconj. J. (IF 2.197) Pub Date : 2020-10-13 Molly D. Pither, Siobhán McClean, Alba Silipo, Antonio Molinaro, Flaviana Di Lorenzo
Pandoraea sp. is an emerging Gram-negative pathogen in cystic fibrosis causing severe and persistent inflammation and damage of the lungs. The molecular mechanisms underlying the high pathogenicity of Pandoraea species are still largely unknown. As Gram-negatives, Pandoraea sp. express lipopolysaccharides (LPS) whose recognition by the host immune system triggers an inflammatory response aimed at the
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TF-containing MUC1 glycopeptides fail to entice Galectin-1 recognition of tumor-associated Thomsen-Freidenreich (TF) antigen (CD176) in solution Glycoconj. J. (IF 2.197) Pub Date : 2020-10-01 Forrest G. FitzGerald, Maria C. Rodriguez Benavente, Camelia Garcia, Yaima Rivero, YashoNandini Singh, Hongjie Wang, Gregg B. Fields, Maré Cudic
Aberrant Mucin-1 (MUC1) glycosylation with the Thomsen-Friedenreich (TF) tumor-associated antigen (CD176) is a hallmark of epithelial carcinoma progression and poor patient prognosis. Recognition of TF by glycan-binding proteins, such as galectins, enables the pathological repercussions of this glycan presentation, yet the underlying binding specificities of different members of the galectin family
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Effects of extraction methods on antioxidant and immunomodulatory activities of polysaccharides from superfine powder Gynostemma pentaphyllum Makino Glycoconj. J. (IF 2.197) Pub Date : 2020-09-29 Bulei Wang, Junfeng Niu, Bingjie Mai, Fangya Shi, Meng Li, Lijun Chen, Pan Wang, Quanhong Liu
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Metabolic transit of dietary advanced glycation end-products - the case of N Ɛ -carboxymethyllysine Glycoconj. J. (IF 2.197) Pub Date : 2020-09-29 Frederic J. Tessier, Eric Boulanger, Michael Howsam
The Maillard reaction, also called glycation, is one of the major chemical reactions responsible for most yellow-to-brown colors and aromas in cooked foods. This reaction between reducing sugars and amino functions on proteins affects not only the flavor of food, but also leads to the formation of an heterogenous group of structurally-modified amino acids. Some of these, known as “advanced glycation
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Identification of novel fish sialidase genes responsible for KDN-cleaving activity. Glycoconj. J. (IF 2.197) Pub Date : 2020-09-27 Kazuhiro Shiozaki,Keiya Uezono,Go Hirai,Akinobu Honda,Masaya Minoda,Ryuta Wakata
2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) is a minor component of sialic acids detected in vertebrates, such as human cancer cells, rat liver, and fish tissues. Although the enzyme activity of KDN-cleaving sialidase (KDN-sialidase) has been detected in rainbow trout, the gene responsible for its expression has not been identified in vertebrates. We evaluated sialidases in human and various
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Xylosyltransferase 2 deficiency and organ homeostasis. Glycoconj. J. (IF 2.197) Pub Date : 2020-09-23 Beatrix Ferencz,Eduard Condac,Nabin Poudel,Maria Cristina Munteanu,Pulavendran Sivasami,Biswa Choudhury,Nandita Natasha Naidu,Fuming Zhang,Melanie Breshears,Robert J Linhardt,Myron E Hinsdale
In this paper we characterize the function of Xylosyltransferase 2 (XylT2) in different tissues to investigate the role XylT2 has in the proteoglycan (PG) biochemistry of multiple organs. The results show that in all organs examined there is a widespread and significant decrease in total XylT activity in Xylt2 knock out mice (Xylt2−/−). This decrease results in increased organ weight differences in
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Aberration of Serum and Tissue N-Glycans in Mouse β1,4-GalT1 Y286L Mutant Variants. Glycoconj. J. (IF 2.197) Pub Date : 2020-09-14 Ran Cao,Tian-Chan Zhang,Ya-Ran Chen,Cui Cao,Huan Chen,Yi-Fan Huang,Morihisa Fujita,Li Liu,Josef Voglmeir
β1,4-GalT1 is a type II membrane glycosyltransferase. It catalyzes the production of lactose in the lactating mammary gland and is supposedly also involved in the galactosylation of terminal GlcNAc of complex-type N-glycans. In-vitro studies of the bovine β4Gal-T1 homolog showed that replacing a single residue of tyrosine with leucine at position 289 alters the donor substrate specificity from UDP-Gal
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APOL1 polymorphism modulates sphingolipid profile of human podocytes. Glycoconj. J. (IF 2.197) Pub Date : 2020-09-11 Manuela Valsecchi,Valentina Cazzetta,Ferdinando Oriolo,Xiqian Lan,Rocco Piazza,Moin A Saleem,Pravin C Singhal,Domenico Mavilio,Joanna Mikulak,Massimo Aureli
Apolipoprotein L1 (APOL1) wild type (G0) plays a role in the metabolism of sphingolipids, glycosphingolipids, sphingomyelin and ceramide, which constitute bioactive components of the lipid rafts (DRM). We asked whether APOL1 variants (APOL1-Vs) G1 and G2 carry the potential to alter the metabolism of sphingolipids in human podocytes. The sphingolipid pattern in HPs overexpressing either APOL1G0 or
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HILIC-UPLC-MS for high throughput and isomeric N-glycan separation and characterization in Congenital Disorders Glycosylation and human diseases. Glycoconj. J. (IF 2.197) Pub Date : 2020-09-11 Angela Messina,Angelo Palmigiano,Francesca Esposito,Agata Fiumara,Andrea Bordugo,Rita Barone,Luisa Sturiale,Jaak Jaeken,Domenico Garozzo
N-glycan analyses may serve uncovering disease-associated biomarkers, as well as for profiling distinctive changes supporting diagnosis of genetic disorders of glycan biosynthesis named congenital disorders of glycosylation (CDG). Strategies based on liquid chromatography (LC) preferentially coupled to electrospray ionization (ESI) - mass spectrometry (MS) have emerged as powerful analytical methods
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Browning and pigmentation in food through the Maillard reaction. Glycoconj. J. (IF 2.197) Pub Date : 2020-09-10 Masatsune Murata
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130 years of Plant Lectin Research. Glycoconj. J. (IF 2.197) Pub Date : 2020-08-29 Mariya Tsaneva,Els J M Van Damme
Lectins are proteins with diverse molecular structures that share the ability to recognize and bind specifically and reversibly to carbohydrate structures without changing the carbohydrate moiety. The history of lectins started with the discovery of ricin about 130 years ago but since then our understanding of lectins has dramatically changed. Over the years the research focus was shifted from ‘the
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N-glycosylation of the human β1,4-galactosyltransferase 4 is crucial for its activity and Golgi localization. Glycoconj. J. (IF 2.197) Pub Date : 2020-08-22 Auhen Shauchuk,Bożena Szulc,Dorota Maszczak-Seneczko,Wojciech Wiertelak,Edyta Skurska,Mariusz Olczak
β1,4-galactosyltransferase 4 (B4GalT4) is one of seven B4GalTs that belong to CAZy glycosyltransferase family 7 and transfer galactose to growing sugar moieties of proteins, glycolipids, glycosaminoglycans as well as single sugar for lactose synthesis. Herein, we identify two asparagine-linked glycosylation sites in B4GalT4. We found that mutation of one site (Asn220) had greater impact on enzymatic
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Sialidase substrates for Sialdiase assays - activity, specificity, quantification and inhibition. Glycoconj. J. (IF 2.197) Pub Date : 2020-08-19 Lei Yuan,Yu Zhao,Xue-Long Sun
Sialidases are glycosidases responsible for the removal of sialic acid (Sia) residues (desialylation) from glycan portions of either glycoproteins or glycolipids. By desialylation, sialidases are able to modulate the functionality and stability of the Sia-containing molecules and are involved in both physiological and pathological pathways. Therefore, evaluation of sialidase activity and specificity
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Monomeric C-mannosyl tryptophan is a degradation product of autophagy in cultured cells. Glycoconj. J. (IF 2.197) Pub Date : 2020-08-17 Shiho Minakata,Yoko Inai,Shino Manabe,Kazuchika Nishitsuji,Yukishige Ito,Yoshito Ihara
C-Mannosyl tryptophan (C-Man-Trp) is a unique glycosylated amino acid present in various eukaryotes. The C-Man-Trp structure can be found as a monomeric form or a part of post-translational modifications within polypeptide chains in living organisms. However, the mechanism of how monomeric C-Man-Trp is produced has not been fully investigated. In this study, we assessed levels of cellular C-Man-Trp
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Comparison of polysaccharide glycoconjugates as candidate vaccines to combat Clostridiodes (Clostridium) difficile. Glycoconj. J. (IF 2.197) Pub Date : 2020-08-13 A D Cox,F St Michael,A Aubry,P C R Strong,A C Hayes,S M Logan
Two known Clostridiodes (Clostridium) difficile surface antigens, a lipoteichoic acid (LTA) and a polysaccharide (PS-II) were isolated and purified in order to prepare glycoconjugate vaccines to the carrier protein human serum albumin utilising a reductive amination strategy. Mice and rabbits were immunized with a prime and two boost strategy and the resulting sera were examined for their ability to
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Chemical O-sulfation of N-sulfoheparosan: a route to rare N-sulfo-3-O-sulfoglucosamine and 2-O-sulfoglucuronic acid. Glycoconj. J. (IF 2.197) Pub Date : 2020-08-10 Lufeng Yan,Paul Brodfueher,Li Fu,Fuming Zhang,Shiguo Chen,Jonathan S Dordick,Robert J Linhardt
Heparosan, the capsular polysaccharide of E. coli K5 is currently used as the starting material in the chemoenzymatic synthesis of heparan sulfate and the structurally related anticoagulant drug heparin. Base hydrolysis of N-acetyl groups and their subsequent N-sulfonation, are used to prepare N-sulfoheparosan an intermediate of biosynthesis. In the present study, when excess sulfonation reagent was
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N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit. Glycoconj. J. (IF 2.197) Pub Date : 2020-08-07 Karina Biskup,Véronique Blanchard,Paola Castillo-Binder,Henry Alexander,Kurt Engeland,Sindy Schug
The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy. hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB). The beta-hCG protein is encoded by CGB3, CGB5, CGB7 and CGB8 genes. CGB3, 5 and 8 code for an identical protein, CGB3/5/8, whereas CGB7 differs in three amino acids from CGB3/5/8. We had observed earlier that CGB7
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Sphingolipids and plasma membrane hydrolases in human primary bronchial cells during differentiation and their altered patterns in cystic fibrosis. Glycoconj. J. (IF 2.197) Pub Date : 2020-07-14 Nicoletta Loberto,Giulia Mancini,Rosaria Bassi,Emma Veronica Carsana,Anna Tamanini,Nicoletta Pedemonte,Maria Cristina Dechecchi,Sandro Sonnino,Massimo Aureli
Human primary bronchial epithelial cells differentiated in vitro represent a valuable tool to study lung diseases such as cystic fibrosis (CF), an inherited disorder caused by mutations in the gene coding for the Cystic Fibrosis Transmembrane Conductance Regulator. In CF, sphingolipids, a ubiquitous class of bioactive lipids mainly associated with the outer layer of the plasma membrane, seem to play
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Differences in anti-inflammatory properties of water soluble and insoluble bioactive polysaccharides in lipopolysaccharide-stimulated RAW264.7 macrophages. Glycoconj. J. (IF 2.197) Pub Date : 2020-07-14 Chun-Han Su,Yun-Ting Tseng,Kai-Yin Lo,Ming-Nan Lai,Lean-Teik Ng
β-Linked polysaccharides including β-glucans are well known to be important functional ingredients, and are known to possess immunomodulatory and anti-tumor activities. This study aimed to investigate the anti-inflammatory properties and participating receptor of water soluble and insoluble bioactive polysaccharides from Grifola frondosa (GFP, non-digestible water soluble polysaccharides), Laminaria
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Structural characteristics and anti-complement activities of polysaccharides from Sargassum hemiphyllum. Glycoconj. J. (IF 2.197) Pub Date : 2020-07-02 Weihua Jin,Qiufu Fang,Di Jiang,Tongtong Li,Bin Wei,Jiadong Sun,Wenjing Zhang,Zhongshan Zhang,Fuming Zhang,Robert J Linhardt,Hong Wang,Weihong Zhong
Three polysaccharides (SH-1, SH-2 and SH-3) were purified from a brown macroalgea, Sargassum hemiphyllum. The autohydrolysis products from each polysaccharide were separated to three fractions (S fractions as oligomers, L fractions as low molecular weight polysaccharides and H fractions as high molecular weight polysaccharides). Mass spectroscopy of S fractions (SH-1-S, SH-2-S and SH-3-S) showed that
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Straightforward synthesis of the pentasaccharide repeating unit of the cell wall O-antigen of Escherichia coli O43 strain. Glycoconj. J. (IF 2.197) Pub Date : 2020-06-29 Pradip Shit,Anup Kumar Misra
A concise synthetic strategy has been developed for the synthesis of the pentasaccharide repeating unit of the cell wall O-antigen of Escherichia coli O43 strain involving stereoselective β-D-mannosylation and α-L-fucosylation using corresponding trichloroacetimidate intermediates and perchloric acid supported over silica (HClO4-SiO2) as glycosylation promoter. The yield and stereoselectivity of the
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Click chemistry compared to thiol chemistry for the synthesis of site-selective glycoconjugate vaccines using CRM197 as carrier protein. Glycoconj. J. (IF 2.197) Pub Date : 2020-06-13 G Stefanetti,M Allan,A Usera,F Micoli
Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures. Herein, different conjugation methods were investigated with the aim of controlling glycosylation site and glycosylation density on the carrier
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The expression and functional analysis of the sialyl-T antigen in prostate cancer. Glycoconj. J. (IF 2.197) Pub Date : 2020-06-24 Ruifeng Bai,Xue Luan,Yu Zhang,Catherine Robbe-Masselot,Inka Brockhausen,Yin Gao
Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer. In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145)
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The preparation of a cold-water soluble polysaccharide from Grifola frondosa and its inhibitory effects on MKN-45 cells. Glycoconj. J. (IF 2.197) Pub Date : 2020-06-15 Chao Liu,Hai-Yu Ji,Peng Wu,Juan Yu,An-Jun Liu
In this study, a novel water soluble polysaccharide (named GFP-4) was extracted from Grifola frondosa at 4 oC, and its preliminary structure and inhibitory effects on human gastric carcinoma MKN-45 cells through the Fas/FasL death receptor apoptosis pathway were investigated. High-performance gel permeation chromatography (HPGPC), fourier-transform infrared spectroscopy (FT-IR), and ion chromatography
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Identification by mass spectrometry and immunoblotting of xenogeneic antigens in the N- and O-glycomes of porcine, bovine and equine heart tissues. Glycoconj. J. (IF 2.197) Pub Date : 2020-06-15 Chunsheng Jin,Reeja Maria Cherian,Jining Liu,Heribert Playà-Albinyana,Cesare Galli,Niclas G Karlsson,Michael E Breimer,Jan Holgersson
Animal bioprosthetic heart valves (BHV) are used to replace defective valves in patients with valvular heart disease. Especially young BHV recipients may experience a structural valve deterioration caused by an immune reaction in which α-Gal and Neu5Gc are potential target antigens. The expression of these and other carbohydrate antigens in animal tissues used for production of BHV was explored. Protein
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Impact of glycoscience in fighting Covid-19. Glycoconj. J. (IF 2.197) Pub Date : 2020-08-01 Roberto Adamo,Sandro Sonnino
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Construction and evaluation of an antibody phage display library targeting heparan sulfate. Glycoconj. J. (IF 2.197) Pub Date : 2020-05-28 Lars A A Damen,Els M A van de Westerlo,Elly M M Versteeg,Thierry van Wessel,Willeke F Daamen,Toin H van Kuppevelt
Heparan sulfate (HS) is a linear polysaccharide with high structural diversity. Different HS epitopes have been detected and localized using single chain variable fragment (scFv) antibodies from a ‘single pot’ phage display library containing a randomized complementarity determining region of the heavy chain (CDR3). In this study, we created a new library containing anti-HS scFvs that all harbor a
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Obituary for Roland Schauer. Glycoconj. J. (IF 2.197) Pub Date : 2020-06-01 Hans-Christian Siebert,Tibor Kožár,Athanasios K Petridis
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Identification of novel glycosylation events on human serum-derived factor IX. Glycoconj. J. (IF 2.197) Pub Date : 2020-05-06 Cassandra L Pegg,Lucia F Zacchi,Dinora Roche Recinos,Christopher B Howard,Benjamin L Schulz
Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade. Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates. Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously
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Identification of lipid raft glycoproteins obtained from boar spermatozoa. Glycoconj. J. (IF 2.197) Pub Date : 2020-05-04 José Benito López-Salguero,Reyna Fierro,Jean-Claude Michalski,Irma Jiménez-Morales,Tony Lefebvre,Oscar Mondragón-Payne,Steffi F Baldini,Anne-Sophie Vercoutter-Edouart,Humberto González-Márquez
The surface of the spermatozoa is coated with glycoproteins the redistribution of which during in vitro capacitation plays a key role in the subsequent fertilization process. Lipid rafts are membrane microdomains involved in signal transduction through receptors and include or recruit specific types of proteins and glycoproteins. Few studies have focused on identifying glycoproteins resident in the
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A core fucose specific lectin from Cephalosporium curvulum induces cellular apoptosis in hepatocellular and pancreatic cancer cells and effective in detecting AFP. Glycoconj. J. (IF 2.197) Pub Date : 2020-05-04 Shivakumar Belur,Narasimhappagari Jagadeesh,Bale M Swamy,Shashikala R Inamdar
Cephalosporium curvulum lectin (CSL), a lectin from pathogenic fungus has exquisite specificity towards α1–6 linkage of core fucosylated glycans, expressed in hepatocellular and pancreatic cancer. Interaction and effect of CSL and other fucose specific lectins LCA and AOL on HepG2 and PANC-1 cells was investigated. CSL, LCA and AOL exhibited strong binding to PANC-1 cells which could be effectively
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Synthesis of the Thomsen-Friedenreich-antigen (TF-antigen) and binding of Galectin-3 to TF-antigen presenting neo-glycoproteins. Glycoconj. J. (IF 2.197) Pub Date : 2020-05-04 Marius Hoffmann,Marc R Hayes,Jörg Pietruszka,Lothar Elling
The Thomsen-Friedenreich-antigen, Gal(β1–3)GalNAc(α1-O-Ser/Thr (TF-antigen), is presented on the surface of most human cancer cell types. Its interaction with galectin 1 and galectin 3 leads to tumor cell aggregation and promotes cancer metastasis and T-cell apoptosis in epithelial tissue. To further explore multivalent binding between the TF-antigen and galectin-3, the TF-antigen was enzymatically
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Structural characterization and antioxidant potential of a novel exopolysaccharide produced by Bacillus velezensis SN-1 from spontaneously fermented Da-Jiang. Glycoconj. J. (IF 2.197) Pub Date : 2020-04-15 Chengxu Cao,Yiming Liu,Yang Li,Yan Zhang,Yue Zhao,Rina Wu,Junrui Wu
The aim of this study was to characterize the exopolysaccharide (EPS) produced by Bacillus velezensis SN-1 (B. velezensis SN-1) (EPS-SN-1), which was isolated from the fermented Da jiang. The microbe made crude exopolysaccharides EPS-SN-1 was produced throughout the bacterial growth period, and the highest yield (2.7 g/L) was obtained with sucrose as the carbon source. As per high performance liquid
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The oligosaccharide portion of ganglioside GM1 regulates mitochondrial function in neuroblastoma cells. Glycoconj. J. (IF 2.197) Pub Date : 2020-04-08 Maria Fazzari,Matteo Audano,Giulia Lunghi,Erika Di Biase,Nicoletta Loberto,Laura Mauri,Nico Mitro,Sandro Sonnino,Elena Chiricozzi
The crucial role of ganglioside GM1 in the regulation of neural homeostasis has been assessed by several studies. Recently we shed new light on the molecular basis underlying GM1 effects demonstrating that GM1 oligosaccharide directly binds TrkA receptor and triggers MAPK pathway activation leading to neuronal differentiation and protection. Following its exogenous administration, proteomic analysis
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Disruption of hepatocyte Sialylation drives a T cell-dependent pro-inflammatory immune tone. Glycoconj. J. (IF 2.197) Pub Date : 2020-03-28 Douglas M Oswald,Julie Y Zhou,Mark B Jones,Brian A Cobb
Through the catalysis of α2,6-linked sialylation, the enzyme ST6Gal1 is thought to play key roles in immune cell communication and homeostasis. Of particular importance, glycans with terminal α2,6-sialic acids are known to negatively regulate B cell receptor signaling and are associated with an immunosuppressive tumor microenvironment that promotes T cell anergy, suggesting that α2,6-sialic acids are
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Gangliosides in the differentiation process of primary neurons: the specific role of GM1-oligosaccharide. Glycoconj. J. (IF 2.197) Pub Date : 2020-03-20 Erika Di Biase,Giulia Lunghi,Maria Fazzari,Margherita Maggioni,Diego Yuri Pomè,Manuela Valsecchi,Maura Samarani,Pamela Fato,Maria Grazia Ciampa,Simona Prioni,Laura Mauri,Sandro Sonnino,Elena Chiricozzi
It has been recently reported by our group that GM1-oligosaccharide added to neuroblastoma cells or administered to mouse experimental model mimics the neurotrophic and neuroprotective properties of GM1 ganglioside. In addition to this, differently from GM1, GM1-oligosaccharide is not taken up by the cells, remaining solubilized into the extracellular environment interacting with cell surface proteins
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