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Development and Validation of a New Reversed Phase HPLC Method for the Quantitation of Azithromycin and Rifampicin in a Capsule Formulation J Chromatogr Sci (IF 1.3) Pub Date : 2024-03-17 Foram Patel, Rajendra Kotadiya, Rashmin Patel, Mrunali Patel
This research aimed to develop a new method for simultaneously estimating the presence of azithromycin (AZT) and rifampicin (RIF) in a capsule formulation using reverse-phase high-performance liquid chromatography. The developed method utilized a Gemini column with a 60:40% v/v acetonitrile and potassium dihydrogen phosphate mobile phase, a flow rate of 1 mL/min, and an injection volume of 20 μL. The
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Development and Validation of a New High-Performance Liquid Chromatography-Ultraviolet Assay for Quantification of Mitoxantrone in Plasma of BALB/c-nu Mice J Chromatogr Sci (IF 1.3) Pub Date : 2024-03-17 Yanru Tao, Hua Zhao, Yujie Xiang, Jin Li, Yanting Li, Jiangling Hu, Hongmei Wang, Xinhui Jiang
The concentration of mitoxantrone in the blood of mice was determined by a high-performance liquid chromatography-ultraviolet method with aloe-emodin as the internal standard. The separation was performed on a Hypersil BDS2 column (4.6 × 250 mm, 5 μm) as the analytical column, the mobile Phase A was acetonitrile, and B was 20-mM potassium dihydrogen phosphate (adding 1% triethylamine and adjusting
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New Quality Evaluation of Qizhi Xiangfu Pills Based on Fingerprint with Chemometric Analysis and Quantitative Analysis of Multi-Components by Single Marker J Chromatogr Sci (IF 1.3) Pub Date : 2024-03-06 Xiaoli Xu, Lin Yang, Dezhang Zhao, Yiwu Wang, Lei Dai, Shuya Li, Dan He
Qizhi Xiangfu Pills (QZXFPs) is one of the most commonly used traditional Chinese medicine preparations for the treatment of dysmenorrhea, but the existing quality evaluation standards have certain shortcomings and deficiencies. An effective and scientific quality evaluation method plays a vital role in medication safety. In this study, fingerprint combined with chemometric analysis and quantitative
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Green Chromatographic Assay of Toxic Impurities of Cyproheptadine: In Silico Toxicity Profiling J Chromatogr Sci (IF 1.3) Pub Date : 2024-02-26 Raghda A Emam, Aml A Emam
Cyproheptadine (CYP) is an antihistaminic and anti-serotonin drug used to improve the clinical course of hospitalized patients severely affected by COVID-19 and requiring supplemental oxygen. Thin layer chromatography (TLC)-densitometry method was developed and validated for the estimation of CYP and its impurity, dibenzosuberone and its degradation product: 10, 11-dihydroxy-dibenzosuberone. The toxicity
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Comparison of Plasma Concentration of Linezolid’s Detection by FICA and LC–MS/MS J Chromatogr Sci (IF 1.3) Pub Date : 2024-02-16 Jing Chen, Chenxia Zhu, Yangjin He, Hengbin Cao
Linezolid was the first totally synthetic oxazolidinone antibiotic that was approved for clinical use against Mycobacterium tuberculosis. Our study investigated the correlation and consistency of fluorescence immunochromatography assay (FICA) and high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) in measuring linezolid levels in the plasma. Plasma samples were collected from
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Structural Elucidation of Novel Degradation Impurities of Ibrutinib in Ibrutinib Tablets Using Preparative Chromatography, LCMS, HRMS and 2D NMR Techniques J Chromatogr Sci (IF 1.3) Pub Date : 2024-02-09 Rajender Reddy Yerla, Surendrababu Manubolusurya, Saravanakumar Meganathan, Veerababu Madalapu, Gopal Vaidyanathan
Ibrutinib is an orally administered compound that functions as an irreversible covalent inhibitor of the Bruton tyrosine kinase, an essential element in multiple cellular processes including B-cell differentiation, proliferation, migration, survival and apoptosis. The compound has been found to demonstrate efficacy against a range of B-cell malignancies. The drug product is available in oral tablet
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An Eco-Friendly RP-HPLC Method Development and Validation for Quantification of Favipiravir in Bulk and Tablet Dosage Form Followed by Forced Degradation Study J Chromatogr Sci (IF 1.3) Pub Date : 2024-01-24 Monika B Sangani, Nirav Patel
In this work, an eco-friendly simple, precise reverse phase high-performance liquid chromatography (HPLC) method has been developed and validated for Favipiravir in bulk and tablet dosage form followed by its force degradation study. The proposed method was validated to obtain official requirements including stability, accuracy, precision, linearity, robustness and selectivity as per International
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Chemical Profiling of Shen-Wu-Yi-Shen Tablets Using UPLC-Q-TOF-MS/MS and Its Quality Evaluation Based on UPLC-DAD Combined with Multivariate Statistical Analysis J Chromatogr Sci (IF 1.3) Pub Date : 2024-01-22 Yudan Mei, Yumei Hu, Xiaoqian Tao, Jing Shang, Mengyu Qian, Fengtai Suo, Jifeng Li, Liang Cao, Zhenzhong Wang, Wei Xiao
Shen-Wu-Yi-Shen tablets (SWYST) is a traditional Chinese medicine prescription used for treating chronic kidney disease (CKD). This study aims to characterize the constituents in SWYST and evaluate the quality based on the quantification of multiple bioactive components. SWYST samples were analyzed with ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS)
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Determination of Whole Molecular of Thermostable Direct Hemolysins in Milk Powder by HPLC-ESI-TOF J Chromatogr Sci (IF 1.3) Pub Date : 2024-01-12 Hong-na Li, Tao Wang, Zhao-di Kang, Yan-ge Yang, Tao Li, Fei Yuan
Although Vibrio parahaemolyticus (V. parahaemolyticus) is a pathogen frequently found in seafood, there is a possibility of its presence in other foods, such as dairy products. The main virulence factors of V. parahaemolyticus are thermostable direct hemolysins (TDHs) which are lethal toxins, so it is necessary to establish qualitative and quantitative methods for determining TDHs. HPLC-ESI-TOF was
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Study of Pharmacokinetics for Ivermectin B1a from Beagle Dogs J Chromatogr Sci (IF 1.3) Pub Date : 2023-12-22 Yuyang Chen, Xiaofang Huang, Zizheng Guo, Jingyu Zhang, Lixin Zhang, Renke Dai
Ivermectin has been widely used for antiparasitic drug, and has recently shown a broad-spectrum antiviral activity, including anti-Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the pharmacokinetic property of ivermectin has not been fully investigated yet. During the plasma preparation, ~32–46% of ivermectin was found in the precipitation. An Liquid Chromatograph-Mass Spectrometer
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Characterization of the Chemical Constituents in Radix gentianae by Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry J Chromatogr Sci (IF 1.3) Pub Date : 2023-12-20 Xu Pan, Jiao Guan, Yue Cui, Lele Li, Xiaoying Lin, Bo Feng, Heyun Zhu
Radix gentianae (RG) is a traditional Chinese medicine used for the treatment of acute and chronic hepatitis in clinic. However, the chemical profile of RG is still unconfirmed, which hindered the progress of pharmacological study and clinical application. In this study, ultra-high performance liquid chromatography together with quadrupole time-of-flight mass spectrometry techniques were employed to
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Ecofriendly HPLC Method for Simultaneous Determination of the Co-Prescribed Drugs in Chemotherapy Omeprazole, Ondansetron and Deflazacort in Spiked Human Plasma J Chromatogr Sci (IF 1.3) Pub Date : 2023-11-24 Fatma F Abdallah, M Fatma, Ahmed M Gouda, Eglal A Abdelaleem, Aml A Emam
Green, selective and accurate high-performance liquid chromatography (HPLC) chromatographic method is presented for simultaneous separation and quantitation of the co-prescribed drugs in chemotherapy omeprazole, ondansetron and deflazacort in spiked human plasma. An isocratic HPLC separation was performed on X Bridge C18 (4.6 × 250 mm) column with 5 μm particle size using mobile phase consisting of
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A Simple and Rapid High-Performance Liquid Chromatography Method for Preparation and Content Detection of the Mainly Numbing Taste Substances of Zanthoxylum bungeanum Maxim J Chromatogr Sci (IF 1.3) Pub Date : 2023-11-17 Zixu Wang, Yue Liu, Guoqing Sun, Liu Yang, Shuai Huang, Lin Chen, Xianli Zhou
As the characteristic numbing taste substances, hydroxyl-α-sanshool (HAS) and hydroxyl-β-sanshool (HBS) were considered vital indicators to evaluate the quality of Zanthoxylum bungeanum Maxim. However, it is very difficult to obtain their high-purity monomers individually, as the only difference between HAS and HBS is that C-6 cis-trans isomerism. In our study, a simple and rapid Ag +-HPLC method was
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Derivatization-Based Novel Chromatographic and Spectrophotometric Methods for the Simultaneous Determination of Gymnemic Acid and Resveratrol in Antidiabetic Polyherbal Formulation. J Chromatogr Sci (IF 1.3) Pub Date : 2023-11-15 Dhruv Joshi,Charmy Kothari,Nrupesh Patel
Herbal medicine is widely used for the treatment and prevention of various ailments, highlighting the importance of ensuring its consistency and quality. This research focuses on the simultaneous detection of Gymnemic acid (GYM) and Resveratrol (RES) in an antidiabetic polyherbal formulation as no reported method exists for their simultaneously detection. The objective of this study is to develop and
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Simultaneous Determination of General Active Ingredients in Topical Formulations by Gas Chromatography with Flame Ionization Detection (GC-FID). J Chromatogr Sci (IF 1.3) Pub Date : 2023-11-14 Anna Ioutsi,Anastasia Sarnitskaya,Mikhail Sumtsov
A simple, fast, precision and sensitive gas chromatographic method was developed for determination of camphor, L-menthol, methyl salicylate, salicylic acid, benzyl nicotinate and nonivamide in topical formulations. The possibility of determination low-boiling components and high-boiling components by a single analytical method was shown. The method was optimized by evaluating the effect of a large
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Rapid Identification of Alkaloids and Flavonoids in Fissistigma oldhamii var. longistipitatum by Ultra High-Performance Liquid Chromatography and Quadrupole Time-of-Flight Tandem Mass Spectrometry. J Chromatogr Sci (IF 1.3) Pub Date : 2023-11-05 Huizhen Liu,Jiaqian Chen,Cheng Jun Yuan,Junwei He,Heying Chen,Chen Jin,Qiang Guo,Huilian Huang
The herb Fissistigma oldhamii var. longistipitatum has been used for a long time in Asian folk medicine in the treatment of several diseases, including rheumatoid arthritis and other inflammatory conditions. Researchers in China and elsewhere have analyzed and characterized its chemical content. In this study, a UHPLC-Q-TOF-MS/MS method, run in both positive and negative modes, was used to identify
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Simultaneous Determination of Thirteen Iridoid Glycosides in Crude and Processed Fructus Corni from Different Areas by UPLC–MS/MS Method J Chromatogr Sci (IF 1.3) Pub Date : 2023-10-24 Fan Zhang, Yu Yan, Jie-Kun Xu, Lin-Mei Zhang, Li Li, Xin Chen, Dong-Xu Li, Ying Peng, Hua Yang, Ling-Zhi Li, Jun He, Jiang Zheng, Wei-Ku Zhang
Fructus Corni (F. Corni) is the dried mature pulp of Cornus officinalis Sieb. et Zucc.(Cornaceae), which is rich in iridoids. In this study, a simple, sensitive and rapid UPLC–MS/MS method was developed for the simultaneous determination of 13 iridoid glycosides of F. Corni from different areas. Specifically, we included five new compounds (cornusdiridoid C, cornusdiridoid E, cornusdiridoid F, 3′′
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A Simple HPLC-UV Method for Ivosidenib Determination in Human Plasma J Chromatogr Sci (IF 1.3) Pub Date : 2023-10-24 Yoshito Gando, Takeo Yasu
Ivosidenib is used for the treatment of acute myeloid leukemia (AML) with isocitrate dehydrogenase 1 (IDH1) mutations. However, increased blood concentrations of ivosidenib are associated with a risk of a prolonged QT interval in patients with AML. Therapeutic drug monitoring in patients with AML with IDH1 mutation offers the potential to improve treatment efficacy while minimizing toxicity. In this
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HPTLC Method Development and Validation for the Simultaneous Estimation of Nortriptyline HCl and Pregabalin in their Combined Dosage Form J Chromatogr Sci (IF 1.3) Pub Date : 2023-10-21 Hinal Harkhani, Kashyap Thummar, Sanjay Chauhan, Jigna Vadalia
Nortriptyline HCl and pregabalin Tablet is used to treat neuropathic pain as well as mental or mood issues such as sadness, mood, feelings, anxiety and tensions. Very few analytical methods are available for the simultaneous estimation of nortriptyline HCl and pregabalin and no reports has been found for HPTLC method. In the current study, a reliable HPTLC method for the simultaneous measurement of
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AGREE and ESA for Greenness Assessment of a Novel Validated RP-HPLC Method for Simultaneous Determination of Aspirin, Warfarin and Clopidogrel in Rat Plasma: Application to Pharmacokinetic Study of the Possible Interaction between the Three Drugs. J Chromatogr Sci (IF 1.3) Pub Date : 2023-10-03 Norhan S AlSawy,Ehab F ElKady,Eman A Mostafa
The green profile of the developed method is assessed and compared with previously reported methods. Percutaneous coronary intervention is a procedure where a catheter is utilized to place a stent in order to facilitate opening of the blood vessels in the heart. Triple antithrombotic therapy includes oral anticoagulation as warfarin and dual antiplatelet therapy (composed of aspirin and clopidogrel
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Multivariate Optimization for Determination of Favipiravir, a SARS-CoV-2 Molecule, by the Reverse-Phase Liquid Chromatographic Method Using a QbD Approach. J Chromatogr Sci (IF 1.3) Pub Date : 2023-10-03 V V Nishanth G,Tatineni Spandana,Chiriki Devi Sri,Varshini Nataraj,P R Hemanth Vikram,B M Gurupadayya
The object of the analytical work is to develop an analytical multivariate optimization for the determination of Favipiravir (FAV), a SARS-CoV-2 molecule, by the reverse-phase liquid chromatographic method using the analytical quality by design approach. FAV is used as an antiviral drug. Box-Behnken design is utilized for the optimization of the experiment and to identify the critical method parameters
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Central Composite Design Expert-Supported RP-HPLC Optimization and Quantitative Evaluation of Efonidipine Hydrochloride Ethanolate & Chlorthalidone in Tablet J Chromatogr Sci (IF 1.3) Pub Date : 2023-09-25 Bhoomi D Patel, Hitesh J Vekaria
Central composite design based RP-HPLC method optimization for the synchronized analysis of Efonidipine Hydrochloride Ethanolate (EFE) and Chlorthalidone (CHL) in tablet. The effective separation was performed using Inertsil ODS C18 column (250 × 4.6 mm, 5 μm), PDA detector with 0.05 M KH2PO4 Buffer (pH 4.5): Acetonitrile (40:60%v/v) mobile phase. Independent variables were investigated include the
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Enhancing Chromatographic Analysis: A Novel Cryo-Enrichment Module for GC Analysis of Terpenes in Cannabis sativa J Chromatogr Sci (IF 1.3) Pub Date : 2023-09-25 Audrius Sadaunykas, Audrius Zolumskis, Simonas Balčiūnas, Evaldas Naujalis
This study presents the development and validation of a gas chromatography (GC) method using cryo-enrichment for the analysis of α-pinene and β-caryophyllene in Cannabis sativa plant. Cryo-enrichment is a method that involves cooling part of the GC column to improve some aspect of chromatographic analysis. The aim of this study was to improve upon previous GC cryo-enrichment prototype and to create
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Correction to: Thiazolidinediones: Recent Development in Analytical Methodologies. J Chromatogr Sci (IF 1.3) Pub Date : 2023-09-14
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Theoretical Study of Non-Isothermal Gradient Elution Liquid Chromatography. J Chromatogr Sci (IF 1.3) Pub Date : 2023-09-09 Nazia Rehman,Ayesha Parveen,Shamsul Qamar
A two-component model of gradient elution chromatography is investigated to theoretically study the effects of simultaneous variations in temperature and solvent strength on the retention behaviors of elution profiles in thermally insulated liquid chromatographic columns. The gradient elution technique is based on the gradual increase or decrease in eluent strength during the chromatographic operation
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UPLC-PDA Approach for Simultaneous Quantification of Elvitegravir, Tenofovir, Emtricitabine and Cobicistat in Tablets: An MS-ESI Study for Degradation Products. J Chromatogr Sci (IF 1.3) Pub Date : 2023-09-08 Rajya Lakshmi Nimmagadda,Sowjanya Gummadi
A simultaneous ultra-performance liquid chromatography photodiode array detection method was developed and validated for Elvitegravir, Tenofovir and Emtricitabine and Cobicistat drugs in tablet dosage forms. Effective liquid chromatographic quantification was achieved using a Luna C18 (100 × 2.6 mm, 1.6 μ) column with an acetonitrile: 0.1% v/v trifluoro acetic acid (80:20% v/v) mobile phase and a photo
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Quality Evaluation of Volatile Oil in Yanyangke Mixture (YM) Based on GC-MS Fingerprint, GC Multicomponent Quantitative Analysis and Chemical Pattern Recognition Analysis. J Chromatogr Sci (IF 1.3) Pub Date : 2023-09-07 Xiaolian Liang,Zhengteng Yang,Jiabao Ma,Minggang Wang,Fengxian Zhao,Chuhui Zhou,Jiaru Feng,Haimei Song,Yong Chen,Xiongbin Gui
Yanyangke mixture (YM) is composed of 12 kinds of traditional Chinese medicine (TCM) used for the treatment of patients with cough, dry throat and other diseases caused by acute or chronic pharyngitis or patients with difficulty in expectoration. With the wide application of YM in clinical practice, its quality control has attracted huge attention. Based on the multi-component characteristics of Chinese
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Technology Transfer of a Validated RP-HPLC Method for the Simultaneous Estimation of Andrographolide and Paclitaxel in Application to Pharmaceutical Nanoformulation. J Chromatogr Sci (IF 1.3) Pub Date : 2023-09-06 Abhiram Kumar,Rafquat Rana,Ravi Saklani,Madhaw Kumar,Pavan Kumar Yadav,Amrendra Tiwari,Manish Kumar Chourasia
Many analytical methods are reported for simultaneous estimation of pharmaceutical dosages form. However, only a few are reproducible at an industrial scale. The proposed research aims to establish a technology transfer (TT) protocol between two laboratories (Lab-X, originator) with binary and (Lab-Y, receiver) with quaternary high-performance liquid chromatography (HPLC) system. Thus, utilizing reverse-phase
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A Novel RP-HPLC Method for Simultaneous Estimation of Vilanterol Trifenatate, Umeclidinium Bromide and Fluticasone Furoate in Inhalation Dry Powder Formulation. J Chromatogr Sci (IF 1.3) Pub Date : 2023-09-06 Shweta Yadav,Vandana Jain,Hemant Magar,M Vijay Kumar,Sayali Warde,Raman Mohan Singh
The dry powder inhalation formulation containing vilanterol trifenatate, umeclidinium bromide and fluticasone furoate intended for the therapy of bronchospasm related to chronic obstructive pulmonary disease and bronchial asthma was selected for the development and validation of a novel, selective, accurate, precise, quick and cost-efficient reversed-phase, high-performance liquid chromatography method
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Simultaneous Determination of Three Flavonoids in Rat Plasma of Masson Pinus Needles (Pinus massoniana L.) Extracts by UHPLC-MS/MS and Application to Pharmacokinetics Studies after Oral and Transdermal Administration. J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-29 Chenjie Xia,Hongliu Zhu,Changhui Hu,Jie Liu,Haibing Hua,Jianqiu Yang,Zhipeng Chen,Yihong Gao
Pinus massoniana needles, a traditional herb, were applied to prevent hair loss in China. Studies available mainly focused on pine needle flavonoids with various biological activities. However, there has been no pharmacokinetics study of the flavonoids from Pinus needles extract. A selective and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was
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Design of Experiment-Based Green UPLC-DAD Method for the Simultaneous Determination of Indacaterol, Glycopyrronium and Mometasone in their Combined Dosage Form and Spiked Human Plasma J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-28 Mohamed Tarek, Nermine S Ghoniem, Maha A Hegazy, Hebatallah A Wagdy
Indacaterol, is an ultra-long-acting β2 agonist, glycopyrronium is a long-acting muscarinic-antagonist and mometasone is a synthetic corticosteroid. They were used recently in combination for the treatment of severe asthma symptoms and chronic obstructive pulmonary disease. In this work, it was the first time to develop a green and environment friendly ultra-performance liquid chromatographic method
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Simultaneous Estimation of Berberine and Piperine in a Novel Nanoformulation for Epilepsy Control via HPLC J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-28 Amira E El-Nahas, Heba M Elbedaiwy, Maged W Helmy, Amal H El-Kamel
Berberine (Brb) and piperine (Pip) are salient examples of bioactive nutraceuticals possessing a promising role in controlling epilepsy. However, during the development of novel nanoformulation that augments their effects, an adequate determination of each one separately was a challenge since they have nearly the same detection wavelength and diverse solubility profiles. Consequently, a tailored high-performance
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Determination of 11 Kinds of Hair Dyes in Hair-Dyeing Products by Liquid Chromatography–Tandem Mass Spectrometry J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-28 Yu-Xiang Gu, Li-Song Chen, Lei Nie
Eleven kinds of hair dyes were determined in hair-dyeing products by liquid chromatography–tandem mass spectrometry (MS). The samples were extracted with ultrasound in methanol for 20 min. After centrifugation, the supernatant was diluted with 10% methanol/90% water (v/v). Then, the solution was analyzed by Shim-pack Scepter C18–120 column (100 mm × 2.1 mm, 1.9 μm) plus electrospray ionization–MS/MS
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An Analytical Method for Determining N-Nitrosodimethylamine and N-Nitrosodiethylamine Contamination in Irbesartan, Olmesartan and Metformin by UPLC-APCI-MS/MS in Tablet Dosage Form J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-25 Ahmad A Deeb, Mohammad Hailat, Hani M Aldoqum, Mohammad Abuothman, Omar Abuyaman, Wael Abu Dayyih
N-nitrosamine pollutants are probable carcinogens. Regulatory agencies declared their presence in the drugs unsafe for human consumption and demanded their recall. Using ultra-performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (UPLC-APCI-MS/MS) in tablet dosage form based on International Conference on Harmonization (ICH) tripartite guideline criteria
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Using the AQbD Approach, Development and Validation of a Simple, Rapid Stability Indicating Chromatographic Method for Quantification of Related Impurities of Apixaban J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-18 Jayasri Kilari, Pradeep Kumar Brahman
Apixaban is a new oral anticoagulant that has been approved by the United States Food and Drug Administration for the prevention of stroke and other cardiovascular complications in people with non-valvular atrial fibrillation. Then, later, it was given the green light to treat deep venous thrombosis and pulmonary embolism. The biggest challenge in the development of pharmaceutical drugs is comprehending
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Development of Relatively Simple Sample Pretreatment Strategies to Selectively Remove Chromatographic Interfering Peaks of Polysorbate 80 from Liquid Oral Finished Drug Product J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-18 Sarju Adhikari, Shane N Berger, Abu M Rustum
Polysorbate 80 (PS 80) is a nonionic surfactant, used in myriad of pharmaceutical, food and cosmetic formulations. PS 80 components have strong UV absorbance and retain under reversed-phase chromatographic conditions, significantly masking sections of the chromatogram. PS 80-related peaks interferences in a sample are common and can be difficult to separate from the analyte peaks. A liquid oral finished
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A Validated High-Performance Thin-Layer Chromatography Technique for Routine Analysis of Curcumin in Four Different Species of Curcuma Viz. C. amada, C. caesia, C. longa and C. zedoaria J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-18 Avinash Gangal, Manisha Duseja, Neeraj K Sethiya, Dheeraj Bisht, Sushil Kumar Chaudhary, Vijay Singh Rana
In this study, we investigated a new, simple, sensitive, selective and precise high-performance thin-layer chromatography (HPTLC) fingerprint and quantitative estimation method for the routine analysis of curcumin in Curcuma species viz. Curcuma amada, Curcuma caesia, Curcuma longa and Curcuma zedoaria. Linear ascending development was carried out in a twin-trough glass chamber saturated with toluene:acetic
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Ultra-High Performance Liquid Chromatography Method for Bioanalysis of Fampridine Using Dried Blood Spot (DBS) Methodology: Application to Pharmacokinetic Study in Albino Rats J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-12 Sheba M K Chalil, Indukala P Chandrasekharan, Singaram Kathirvel, Raja R Katta
Fampridine (dalfampridine) is used to improve walking in people who have multiple sclerosis (a disease in which the nerves do not function properly and may cause weakness, numbness, loss of muscle coordination and problems with vision, speech and bladder control). Measurement of fampridine plasma concentrations is not practical at sites lacking the facilities to prepare and process blood samples. A
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Box–Behnken Design-Aided Validation and Optimization of a Stability-Indicating Reverse Phase-HPLC Method for the Estimation of Tamoxifen Citrate in Lipidic Nano-Vesicles J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-09 Gautam Kumar, Prashansha Mullick, Krishnadas Nandakumar, Srinivas Mutalik, Mallikarjuna Rao Chamallamudi
Stability indicating a reverse-phase HPLC analytical method for the quantification of tamoxifen citrate (TMX) in the bulk and lipidic nano-vesicles (LNVs) was developed. The optimized method was validated according to the ICH Q2 (R1) guidelines by following a three-factor interaction Box–Behnken design using Design-Expert® software. The responses measured at 236 nm were retention time (Rt), peak area
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Thiazolidinediones: Recent Development in Analytical Methodologies. J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-03 Tarang Patel,Vatsal Patel
The instrumental analytical methods that have been developed and utilized for the determination of thiazolidinedione in bulk medications, formulations and biological fluids have been reviewed after an in-depth analysis of the literature published in a variety of analytical and pharmaceutical chemistry-related journals. The approaches covered by this research, which covers the years 2001-2022, include
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Experimental Design Application for Measuring Histamine in Tuna Fish Samples by Phenyl Isothiocyanate Derivation Method Using Ultra-Performance Liquid Chromatography. J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-02 Maede Ameri,Seyed Mosayeb Daryanavard
Histamine as an important biogenic amino acid was measured in tuna fish samples by ultra-performance liquid chromatography using a phenyl isothiocyanate derivative. Minitab software was used to design the experiment and investigate the effective factors during the process, which includes screening and optimization steps. A partial factorial design was used in the screening stage and a central composite
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Analytical Quality Risk Assessment and Design of Experiments to Green HPTLC Method for Simultaneous Estimation of Sildenafil Citrate and Dapoxetine Hydrochloride. J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-02 Pintu B Prajapati,Bhavesh M Sheta,Veerashakar Pulusu,Shailesh A Shah
A green and robust high-performance thin-layer chromatographic method has been developed for the simultaneous estimation of sildenafil citrate and dapoxetine hydrochloride. A fractional factorial design was applied for analytical quality risk assessment of potential analytical risk factors. The identified critical analytical risk factors were optimized using the design of experiment-based response
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Salting-Out Assisted Liquid–Liquid Microextraction and Reverse-Phase Chromatographic Quantification of Two Neonicotinoid Insecticides from Fruits and Vegetables J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-02 Sumia Akram, Bushra Sultana, Muhammad Rafique Aasi, Muhammad Mushtaq, Rui Hai Liu
The present monograph describes the salting-out assisted liquid–liquid microextraction (SALLME) and reverse-phase high-performance liquid chromatography with diode array detector (RP-HPLC-DAD) based quantification of two frequently applied neonicotinoid insecticides, i.e., acetamiprid (ACE) and imidacloprid (IMD), from selected tropical fruits (citrus and guava) and vegetables (tomato, okra and cauliflower)
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Application of Box–Behnken Design and Response Surface Methodology for Selecting the Optimum RP-HPLC Conditions for the Simultaneous Determination of Paracetamol and Diclofenac Sodium Along With Three Skeletal Muscle Relaxants in Three Different Pharmaceutical Dosage Forms J Chromatogr Sci (IF 1.3) Pub Date : 2023-08-01 Ehab Elkady, Marwa Fouad, Ayoub Mozayad
An isocratic HPLC method has been developed and validated for estimating paracetamol and diclofenac sodium simultaneously with three skeletal muscle relaxants, namely, methocarbamol, tizanidine hydrochloride and chlorzoxazone in their pure standard mixtures and in different multi-component dosage forms in a single chromatographic run. HPLC separation was achieved on a C18 Inertsil ODS-3V 5 μm column
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Implementation of White Analytical Chemistry-Assisted Analytical Quality by Design Approach to Green Liquid Chromatographic Method for Concomitant Analysis of Anti-Hypertensive Drugs in Human Plasma. J Chromatogr Sci (IF 1.3) Pub Date : 2023-07-31 Pintu Prajapati,Abhinandan Shahi,Aneri Acharya,Veera Shakar Pulusu,Shailesh Shah
According to current concepts of white analytical chemistry (WAC), the use of organic solvents those are teratogenic and carcinogenic must be avoided for the protection of the environment and of the analysts. This led to the development and validation of the WAC-assisted green liquid chromatographic technique (reverse-phase high-pressure liquid chromatography (RP-HPLC)) for the simultaneous analysis
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A Sensitive Liquid Chromatography-Mass Spectrometric Method for Determination of Bisoprolol in Rat Serum after Pre-Column Derivatization. J Chromatogr Sci (IF 1.3) Pub Date : 2023-07-28 Huda A Shaheen,Saleh O Bahaffi,Alaa M Khedr
Two derivatization regents were reacted with bisoprolol (BIS), followed by liquid-chromatography-mass spectrometric analysis. 3-Bromomethyl-propyphenazone (BMP) and dansyl-chloride (Dns-Cl) were reacted via the secondary amino group using a catalyst to accelerate the reaction progress and completeness with minimal reaction byproducts. The sensitivity and the ionization efficiency of both BIS-methyl-propyphenazone
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A Novel Method for Determination of Cyclobenzaprine in the Different Matrixes by UHPLC-DAD: Application to Real Human Plasma and Pharmaceutical Formulations. J Chromatogr Sci (IF 1.3) Pub Date : 2023-07-28 Tugrul C Akman
This study presents a novel and efficient method for determining of cyclobenzaprine (CBP) in human plasma and tablets using simple liquid-liquid microextraction and ultra-high performance liquid chromatography-diode-array detector. The developed method was optimized and validated for selectivity, linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The chromatographic
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Development and Validation of a SPE-HPLC Method for Quantification of Rhein, Emodin, Chrysophanol and Physcion in Rhamnus petiolaris Boiss. & Balansa. J Chromatogr Sci (IF 1.3) Pub Date : 2023-07-27 Pelin Köseoğlu Yılmaz,Ufuk Kolak
Anthraquinones exhibit a significant group of natural and synthetic quinone derivatives because of their biological activities and industrial applications. Rhamnaceae is one of the families known to contain different kinds of anthraquinones. In this study, it was aimed to quantify rhein, emodin, chrysophanol and physcion in fruits of Rhamnus petiolaris Boiss. & Balansa belonging to Rhamnaceae by solid
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Development of HPLC-UV Method for Simultaneous Determination of Corticosteroid Co-Administered Immune Therapy J Chromatogr Sci (IF 1.3) Pub Date : 2023-07-20 Nessreen S Abdelhamid, Nehal F Farid, Basma H Anwar, Maimana A Magdy
Prednisolone (PDS) has recently been utilized to treat a variety of medical disorders, including autoimmune illnesses and cancer. It is also used to treat coronavirus disease 2019 infection-related respiratory problems. Because it may induce health problems including gastrointestinal lesions and ulceration, it has to be used alongside other drugs like esomeprazole (ESM), which acts as a proton pump
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Development of HPLC Method for Ixabepilone (Oncology Drug) in Bulk and Dosage Form: Quantification of Impurities and Forced Degradation Studies. J Chromatogr Sci (IF 1.3) Pub Date : 2023-07-18 Arjuna R Nekkalapudi,Srinivasu Navuluri,Sreenivas Pippalla,Naresh K Katari,Vijay K Cholleti,Hanimi R Bapatu
The objective of study is to develop a new stability-indicating HPLC method for quantifying ixabepilone degradation products and known process impurities (EPO-2 and Epothilone B) in bulk and injectable dose forms. A gradient stability-indicating RP-HPLC approach was developed to determine the known impurities of ixabepilone in ixabepilone API and ixabepilone for injection. Ixabepilone was subjected
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Qualitative and Quantitative Analyses of the Enantiomers of Nicotine and Nornicotine Employing Chiral Supercritical Fluid Chromatography. J Chromatogr Sci (IF 1.3) Pub Date : 2023-07-14 Mehdi Ashraf-Khorassani,Thomas A Perfetti,Mike F Dube,William M Coleman,John M Ferraro,Weston J Umstead
An optimized method employing chiral supercritical fluid chromatography with diode array UV-VIS detection has been developed for the quantitative analysis of nicotine and nornicotine enantiomer distributions. The method parameters that were optimized included: column type (stationary phases, Chiralpak IG-3), column temperature (40°C), modifier types and concentration (isopropyl alcohol, 10%), additive
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GC Analysis of Metformin, Ranitidine and Famotidine from Pharmaceuticals and Human Serum J Chromatogr Sci (IF 1.3) Pub Date : 2023-07-07 Rizwan A Zounr, Muhammad Y Khuhawar, Taj M J Khuhawar, Muhammad F Lanjwani, Muzamil Y Khuhawar
A method has been designed based on gas chromatography with flame ionization detection (FID) for the separation and analyses of ranitidine, famotidine and metformin after pre-column derivatization with trifluoroacetylacetone and ethyl chloroformate. DB-1 (30 m × 0.32 mm id) column with film thickness 0.25 μm was used for the separation at an initial temperature of column was 100°C for 2 min, and ramping
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Chromatographic Enantioseparation of Chiral Sulfoximines on Polysaccharide-Based Chiral Stationary Phases. J Chromatogr Sci (IF 1.3) Pub Date : 2023-11-05 Qinqin Tang,Ping Zhao,Qingle Zeng
Chiral sulfoximines have significant roles in pharmaceutical industry and agricultural chemicals. Furthermore, chiral structurally related sulfoximines are used for their wide potential applications in some uncharted territory. However, chromatographic study on these compounds has not been systematically performed. Herein, this paper describes the enantioseparation of 12 chiral sulfoximines on polysaccharide-based
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Validation and Application of UPLC-MS/MS Method to Analysis of Glyphosate and its Metabolites in Water J Chromatogr Sci (IF 1.3) Pub Date : 2023-06-24 Pedro de J. Bastidas-Bastidas, Rosina Cabrera, Ana I Valenzuela-Quintanar, Claudia Olmeda-Rubio, Victor M González-Mendoza, Xiomara P Perea-Domínguez, Luis C González-Márquez, Vilma del C. Salvatierra-Stamp, José B Leyva-Morales
A method was developed to determine glyphosate and their metabolites in water. The widespread use of this herbicide in agricultural activities worldwide, despite the reported adverse effects on both the environment and health, is a cause for concern and makes it necessary to monitor its presence through a method that guarantees the determination at trace levels. A direct extraction of the analytes
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RP-LC Method Development and Validation for Dasatinib Forced Degradation Study: Isolation and Structural Characterization by NMR and HRMS J Chromatogr Sci (IF 1.3) Pub Date : 2023-06-21 Dasameswara Rao Kavitapu, Jayanti Naga Sri Rama Chandra Murty, Arthanareeswari Maruthapillai, Gopal C Senadi, Sudarshan Mahapatra
A reverse phase high-performance liquid chromatography (HPLC) method has been developed for the quantification of a typical drug Dasatinib (DST) and its related impurities in pharmaceuticals. Kinetex C18 (4.6 × 150 mm, 5 μm) column was used in the chromatographic separations, using buffer (1.36 g of KH2PO4 in 1000 mL of water, pH = 7.8; adjusted with diluted KOH solution) with solvent as acetonitrile
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Green TLC-Densitometric Method for Simultaneous Determination of Antazoline and Tetryzoline: Application to Pharmaceutical Formulation and Rabbit Aqueous Humor J Chromatogr Sci (IF 1.3) Pub Date : 2023-06-15 Ola G Hussein, Yasmin Rostom, Mohamed Abdelkawy, Mamdouh R Rezk, Dina A Ahmed
Ophthalmic pharmaceutical preparation containing antazoline (ANT) and tetryzoline (TET) is prescribed widely as an over the counter medication for allergic conjunctivitis treatment. Development of a selective, simple and environmentally friendly thin-layer chromatographic method established to determine both ANT and TET in their pure forms, pharmaceutical formulation and spiked aqueous humor samples
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Determination of Atropine by HPLC in Plant of Datura by Liquid–Liquid Extraction and Magnet Solid-Phase Extraction J Chromatogr Sci (IF 1.3) Pub Date : 2023-06-15 Shaida Mahmoudi, Mohamad Javad Chaichi, Mojtaba Shamsipur, Ome Leila Nazari, Abdol Rauf Samadi Mayodi
Atropine is a tropane alkaloid found in abundance in Datura plant. We used two liquid–liquid extraction methods and magnet solid-phase extraction to compare the amount of atropine in these two types of plants (Datura innoxia and Datura stramonium). The surface magnetic nanoparticle Fe3O4 correction with an amine and dextrin, and finally, magnetic solid-phase extraction Fe3O4@SiO2-NH2-dextrin (MNPs-dextrin)
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Development of A Liquid-Phase Microextraction Method for Simultaneous Determination of Parabens in Lipstick Samples at Trace Levels by High-Performance Liquid Chromatography. J Chromatogr Sci (IF 1.3) Pub Date : 2023-06-12 Tuğçe U Gösterişli,İrem Z Kublay,Seyfullah Keyf,Sezgin Bakırdere
The endocrine-disrupting potential of parabens, as well as their relation to cancer, has sparked significant discussions over their impact. Consequently, analyses of cosmetic products are an essential necessity, particularly in terms of human health and safety. In this study, a highly accurate and sensitive liquid-phase-based microextraction method was developed to determine the five parabens at trace
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Effect of Vinegar on the Oxidative Stability of Mayonnaise During its Storage J Chromatogr Sci (IF 1.3) Pub Date : 2023-05-24 Afraa Alnokkari
Mayonnaise is an emulsion of oil that is susceptible to lipid oxidation, which can cause spoilage and the formation of harmful compounds. This study aims to evaluate the impact of Syrian apple and grape vinegar on the oxidative stability of mayonnaise and compare the use of natural antioxidants to synthetic ones such as butylated hydroxyanisole and butylated hydroxytoluene. The study measured total