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  • Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via Nrf2 pathway
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-25
    Chao Han; Yuanyuan Wei; Yuqing Cui; Yumemg Geng; Yongzhan Bao; Wanyu Shi

    In order to explore the mechanism of liver injury induced by florfenicol (FFC) in broilers, one hundred and twenty broilers were randomly divided into six groups, twenty broilers in each group. Except for control group, the other five groups were given different doses of FFC (0.15 g/L, 0.3 g/L, 0.6 g/L, 1.2 g/L and 1.8 g/L) in drinking water. After five days of continuous use, blood was collected from the subpterional vein and the chickens' liver were obtained. Chicken weight gain and liver indices were calculated; blood routine analysis was performed; the oxidative stress and apoptosis of hepatocytes was detected. The results showed that compared with the control group, except for 0.15 g/L FFC, the other doses of FFC significantly decreased the weight gain, white blood cell (WBC) and platelet (PLT) contents in blood, 0.3 g/mL FFC and 1.8 g/L FFC significantly reduced the content of hemoglobin (RGB) (P < 0.05); all doses of FFC significant decreased red blood cell (RBC) increased Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) contents in serum of chickens (P < 0.05), and significantly decreased the contents of albumin (ALB) and total protein (TP) in serum (P < 0.05), but had no significant effect on alkaline phosphatase (ALP) contents(P > 0.05). FFC significantly increased malondialdehyde (MDA) content in serum and liver tissues, but decreased glutathione (GSH), Superoxide dismutase (SOD) and catalase (CAT) content (P < 0.05), and significantly inhibited the mRNA transcription and protein expression of antioxidant proteins nuclear factor-erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and NAD(P)H dehydrogenase quinone-1 (NQO-1)(P < 0.05). FFC also inhibited the content and the transcription level of cytochrome P4501A1(CYP1A1) and CYP2H1 in liver (P < 0.05). At the same time, FFC significantly promoted the apoptotic rate of hepatocytes and the mRNA transcription and protein expression of caspase-3 and caspase-6 (P < 0.05). With the increase of FFC concentration, liver injury became more and more serious, which affected liver function in chickens by inhibiting enzyme activity in Nrf2-ARE pathway to increase oxidative stress and promoting apoptotic protein expression to accelerate hepatocyte apoptosis.

    更新日期:2020-01-26
  • EDTA-enhanced phytoremediation of heavy metals from sludge soil by Italian ryegrass (Lolium perenne L.)
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-24
    Fei-li Li; Yuehua Qiu; Xinyang Xu; Feng Yang; Zhiwei Wang; Jianru Feng; Jiade Wang
    更新日期:2020-01-26
  • Seasonal response of the synergism of maternal comorbidities and long-term air pollution exposure on birth outcomes
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-24
    Boyi Zhao; Min Wang; Changwei Lü; Ling Feng; Hua Ma; Haixia Meng; Muge Qi; Qingyun Fan; Haoji Wang; Haijun Zhou; Jiang He
    更新日期:2020-01-26
  • Antibiotic exposure across three generations from Chinese families and cumulative health risk
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-25
    Jingjing Zhang; Xinji Liu; Yitian Zhu; Linsheng Yang; Liang Sun; Rong Wei; Guimei Chen; Qunan Wang; Jie Sheng; Annuo Liu; Fangbiao Tao; Kaiyong Liu
    更新日期:2020-01-26
  • Phosphate microbial mineralization consolidation of waste incineration fly ash and removal of lead ions
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-25
    Xiaoniu Yu; Jianguo Jiang

    This paper proposes a green environment-friendly Bacillus subtilis to mineralize and consolidate waste incineration fly ash and heavy metal cations, and there is no harmful by-product in the mineralization process. Different phosphate products can be prepared, and are more stable than the microbially-induced carbonate precipitation (MICP) in nature. Typical heavy metal oxides were mainly PbO, ZnO, CdO, NiO, CuO and Cr2O3 in the chemical composition of waste incineration fly ash. Microstructure and chemical composition of waste incineration fly ash before and after treatment were characterized by powder X-ray diffraction (XRD) analysis and scanning electron microscopy. Scanning electron microscopy (SEM) images showed that the morphology of the Bacillus subtilis was mainly a rod-like structure. The optimal hydrolysis dosage of the organic phosphate monoester sodium salt was 0.2mol in the bacterial solution (1L, 20 g/L). The optimum required mass of the bacterial powder was 15 g/kg in treatment process of the waste incineration fly ash. The initial concentration of lead ions was 40.28 mg/L in waste incineration fly ash solution. After the optimum dosage treatment, the removal efficiency of lead ions was 78.15%, 79.64%, 77.70% and 80.14% when curing time was 1, 2, 4 and 6d, respectively. The waste incineration fly ash had a Shore hardness of 22 after the optimum amount of bacterial liquid treatment. Results of wind erosion test showed that the wind erosion rate of waste incineration fly ash was 2.6, 0, 0, 0, 0 and 0 g/h when blank group, deionized water, 100, 200, 300 and 400 mL of bacterial solutions treated, respectively. The bio-mineralization method provides an approach for the safe disposal of heavy metals in the contaminated areas of tailings, electroplating sewage, waste incineration plants, and so on.

    更新日期:2020-01-26
  • Promotion effect of liver tumor progression in male kras transgenic zebrafish induced by tris (1, 3-dichloro-2-propyl) phosphate
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-25
    Sheng Chen; Zhiyuan Gong; Robert J. Letcher; Chunsheng Liu

    A previous study reported that exposure to tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) could promote the progression of hepatocellular carcinoma (HCC) in female HCC model zebrafish. Due to the existence of gender disparity in the development of HCC between females and males, whether the promotion effect of TDCIPP still exists in male HCC model zebrafish remains unclear. In this study, Tg(fabp10:rtTA2s-M2; TRE2:EGFP-krasG12V), referred as kras transgenic zebrafish which was shown to be an inducible liver tumor model, was applied as experimental model to assess the promotion potential of TDCIPP for HCC in males. In brief, kras males were exposed to 20 mg/L doxycycline (DOX), 0.3 mg/L TDCIPP and a binary mixture of 20 mg/L DOX with 0.3 mg/L TDCIPP, and after exposure liver size, histopathology and transcriptional profiles of liver from these treatments were examined. With the involvement of TDCIPP, the liver size was significantly increased and the lesion of hepatocyte became more aggressive. Furthermore, expressions of genes involved in DNA replication and inflammatory response were simultaneously up-regulated in the treatment of TDCIPP compared with the solvent control and in the treatment of the binary mixture of the two chemicals compared to the single DOX treatment. Overall, our results suggested that TDCIPP had promotion effect on the progression of liver tumor in kras males.

    更新日期:2020-01-26
  • Profiles of parabens and their metabolites in paired maternal-fetal serum, urine and amniotic fluid and their implications for placental transfer
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-24
    Shiming Song; Yuan He; Tao Zhang; Hongkai Zhu; Xiongfei Huang; Xueyuan Bai; Bo Zhang; Kurunthachalam Kannan
    更新日期:2020-01-26
  • Cytotoxicity and toxicoproteomic analyses of human lung epithelial cells exposed to extracts of atmospheric particulate matters on PTFE filters using acetone and water
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-25
    Zhi-jie Tang; Zhao-ming Cao; Xue-wen Guo; Hong-juan Chen; Yi Lian; Wei-juan Zheng; Yi-jun Chen; Hong-zhen Lian; Xin Hu
    更新日期:2020-01-26
  • Mercury isotope compositions in large anthropogenically impacted Pearl River, South China
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-25
    Yuanyuan Zhang; Jiubin Chen; Wang Zheng; Ruoyu Sun; Shengliu Yuan; Hongming Cai; David Au Yang; Wei Yuan; Mei Meng; Zhongwei Wang; Yulong Liu; Jianfeng Liu

    Rivers integrate natural and anthropogenic mercury (Hg), and are important vectors of terrestrial Hg to the oceans. Here, we report the total Hg concentration and Hg isotope compositions of dissolved load in the Pearl River, the second largest river in China, in order to understand the processes and sources affecting Hg systematics in large anthropogenically-impacted river water. The dissolved Hg showed a concentration varying from 0.45 to 2.44 ng/L, within the range reported for natural background lake and river waters. All river water samples showed significantly negative δ202Hg (−2.89‰ to −0.57‰), slightly positive Δ200Hg (−0.05‰ to 0.52‰), and mostly positive Δ199Hg (0.10‰ to 0.57‰), except for three extremely negative values (−2.25‰ to −0.76‰). Combined with other geochemical parameters, we suggest that the influence of in-river processes, such as sorption and reduction, on the Hg isotope compositions is very limited, and the dissolved Hg in the Pearl River mainly comes from atmospheric precipitation and surface soil weathering. Although the whole river basin is largely affected by urban, industrial and mining activities, unlike other heavy metals, their direct contributions to dissolved Hg seem limited. It is worth noting that the three samples with very negative Δ199Hg values (down to −2.25‰) are derived from special source which attribute to the input of Hg released from the local incineration of electronic wastes. This study demonstrates that isotope approach is a powerful tool for tracing sources and pathways of Hg in large complex river systems.

    更新日期:2020-01-26
  • MnCeOX with high efficiency and stability for activating persulfate to degrade AO7 and ofloxacin
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-24
    Lijun Niu; Guang Xian; Zeqing Long; Guangming Zhang; Jia Zhu; Jinwei Li
    更新日期:2020-01-24
  • In vitro assessment of the combination of cylindrospermopsin and the organophosphate chlorpyrifos on the human neuroblastoma SH-SY5Y cell line
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-23
    M.G. Hinojosa; A.I. Prieto; D. Gutiérrez-Praena; F.J. Moreno; A.M. Cameán; A. Jos

    Cylindrospermopsin (CYN) is a cyanotoxicant which occurrence is increasing due to climate change. Cylindrospermopsin is able to exert damage in the organism at several levels, among them, in the nervous system. Moreover, it is important to take into account that it is not usually present isolated in nature, but in combination with some other pollutants, being the case of the pesticide chlorpyrifos (CPF). Thus, the aim of the present work was to assess the effects of the interaction of CYN in combination with CPF in the human neuroblastoma cell line SH-SY5Y by evaluating cytotoxicity and mechanistic endpoints. The mixtures 0.25 + 21, 0.5 + 42, 1 + 84 μg/mL of CYN + CPF based on cytotoxicity results, were evaluated, and the isobologram method detected an antagonistic effect after 24 and 48 h of exposure. Moreover, although no alterations of reactive oxygen species were detected, a significant decrease of glutathione levels was observed after exposure to both, CPF alone and the combination, at all the concentrations and times of exposure assayed. In addition, CYN + CPF caused a marked decrease in the acetylcholinesterase activity, providing similar values to CPF alone. However, these effects were less severe than expected. All these findings, together with the morphological study results, point out that it is important to take into account the interaction of CYN with other pollutants. Further research is required to contribute to the risk assessment of CYN and other contaminants considering more realistic exposure scenarios.

    更新日期:2020-01-23
  • Salinity influences Cd accumulation and distribution characteristics in two contrasting halophytes, Suaeda glauca and Limonium aureum
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-23
    Songlin Zhang; Xilu Ni; Muhammad Arif; Zhongxun Yuan; Lijuan Li; Changxiao Li

    The potential for the phytoremediation of halophytes has been widely recognized. However, the effects of salt on Cd accumulation characteristics in different halophytic species, which may also be related to their salt tolerance, are still unclear. This study investigated the effects of salinity on Cd accumulation and distribution in two distinct halophytes, Suaeda glauca (euhalophyte) and Limonium aureum (recretohalophyte). Seedlings of the two species were treated with 0, 3, and 6 mg kg−1 soil Cd in combination with or without 0.3% NaCl in a pot experiment. The amount of Cd within the rhizosphere and plant tissues, plant biomass, and the subcellular distribution and chemical forms of Cd were examined. Results showed that the addition of NaCl significantly increased Cd bioavailability at high Cd levels due to the rhizosphere acidification effect. Meanwhile, salinity differently impacted plant biomass allocation, and enhanced Cd uptake and translocation in both studied halophytes. Excess Cd was excreted from the leaf surface, possibly by salt glands of L. aureum, with the salinity facilitating this process. Majority of the Cd was found within the cell walls and vacuolar compartments of two species. However, S. glauca plants had higher proportions of inactive Cd (extracted by 2% HAc and 0.6 M HCl) and lower proportions of active Cd (extracted by 80% ethanol and water), as opposed to L. aureum, which would better inform S. glauca's higher Cd accumulation. Based on these results, S. glauca seems more applicable for phytomanagement of Cd-contaminated saline soils due to its higher capacity for Cd enrichment and tolerance amplified by NaCl.

    更新日期:2020-01-23
  • Nitrate contamination of groundwater in the Lower Volta River Basin of Ghana: Sources and related human health risks
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-22
    Courage D. Egbi; Geophrey K. Anornu; Samuel Y. Ganyaglo; Emmanuel K. Appiah-Adjei; Si-Liang Li; Samuel B. Dampare
    更新日期:2020-01-23
  • 更新日期:2020-01-23
  • Ecological characteristics and teratogenic retinal determination of Cochlodinium geminatum blooms in Pearl River Estuary, South China
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-22
    Yuelei Dong; Lei Cui; Rongbo Cao; Jingyi Cen; Jian Zou; Xiaoyu Zhou; Songhui Lu

    Since 2006, harmful dinoflagellate blooms of Cochlodinium geminatum have infrequently occurred in the Pearl River Estuary, South China. During late October to early November in 2018, C. geminatum blooms occurred again in the region. To investigate the blooming mechanism in certain temporal conditions, we analysed the changes in the environmental parameters and phytoplankton community structure during and after the bloom. The results indicated that the water temperature and salinity had large impacts on the bloom. During the C. geminatum bloom, the phytoplankton community structure changed and the number of dominant species decreased. After the bloom, the species number and abundance of diatoms increased, as the species diversity was recovering. Retinal was detected in the field samples and cultured C. geminatum. It has been demonstrated to exist in some algae species (e.g. Cyanophyta, Chlorophyta, Bacillariophyta, and Euglenophyt), and our results indicates that such teratogens also exist in dinoflagellates. The highest concentration of retinal was detected during the bloom. This result indicates that the retinal content may accumulate during a bloom. Retinal has been demonstrated to be a teratogenic agent and may therefore present a potential risk to aquatic organisms during a bloom episode. This research provided more comprehensive information concerning the ecological influences of C. geminatum blooms.

    更新日期:2020-01-23
  • Influence of pH on the toxicity of ionisable pharmaceuticals and personal care products to freshwater invertebrates
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-21
    Ming Sun; Rahmat Quaigrane Duker; Frits Gillissen; Paul J. Van den Brink; Andreas Focks; Andreu Rico

    The majority of pharmaceuticals and personal health-care products are ionisable molecules at environmentally relevant pHs. The ionization state of these molecules in freshwater ecosystems may influence their toxicity potential to aquatic organisms. In this study we evaluated to what extent varying pH conditions may influence the toxicity of the antibiotic enrofloxacin (ENR) and the personal care product ingredient triclosan (TCS) to three freshwater invertebrates: the ephemeropteran Cloeon dipterum, the amphipod Gammarus pulex and the snail Physella acuta. Acute toxicity tests were performed by adjusting the water pH to four nominal levels: 6.5, 7.0, 7.5 and 8.0. Furthermore, we tested the efficiency of three toxicity models with different assumptions regarding the uptake and toxicity potential of ionisable chemicals with the experimental data produced in this study. The results of the toxicity tests indicate that pH fluctuations of only 1.5 units can influence EC50-48 h and EC50-96 h values by a factor of 1.4–2.7. Overall, the model that only focuses on the fraction of neutral chemical and the model that takes into account ion-trapping of the test molecules showed the best performance, although present limitations to perform risk assessments across a wide pH range (i.e., well above or below the substance pKa). Under such conditions, the model that takes into account the toxicity of the neutral and the ionized chemical form is preferred. The results of this study show that pH fluctuations can have a considerable influence on toxicity thresholds, and should therefore be taken into account for the risk assessment of ionisable pharmaceuticals and personal health-care products. Based on our results, an assessment factor of at least three should be used to account for toxicity differences between standard laboratory and field pH conditions. The models evaluated here can be used to perform refined risk assessments by taking into account the influence of temporal and spatial pH fluctuations on aquatic toxicity.

    更新日期:2020-01-22
  • Indole-3-acetic acid promotes cadmium (Cd) accumulation in a Cd hyperaccumulator and a non-hyperaccumulator by different physiological responses
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-21
    Jiakang Ran; Wen Zheng; Hongbin Wang; Haijuan Wang; Qinchun Li

    To study the effects of indole-3-acetic acid (IAA) on cadmium (Cd) accumulation and the physiological responses of the Cd hyperaccumulator Solanum nigrum and non-hyperaccumulator Solanum melongena, a pot experiment was conducted in soil containing 2 mg kg−1 Cd in which different concentrations of IAA (0, 10, 20, or 40 mg L−1) were sprayed on plant leaves. The results showed that Cd accumulation in shoots of S. nigrum was significantly increased by 30% after the addition of 10 mg L−1 IAA under 2 mg kg−1 Cd stress compared to that in the control, but shoot Cd accumulation showed no significant change in S. melongena after this IAA treatment. Additionally, the growth and the proline content in the two species were significantly increased by 20 mg L−1 IAA. The activities of peroxidase and catalase in leaves of S. nigrum and the activity of superoxide dismutase (SOD) in S. melongena were significantly increased and their malondialdehyde content was significantly decreased compared to those in the control. The root activity of S. nigrum was significantly improved after 10 and 20 mg L−1 IAA treatments, but no significant difference was observed in S. melongena. The correlation analysis results showed that the Cd concentration in leaves of S. nigrum was significantly and positively correlated with the carotenoid and proline contents, and there was also a significant positive correlation between the Cd concentration and SOD activity in leaves of S. melongena. Therefore, S. nigrum is an ideal plant for the phytoextraction of Cd-contaminated soil assisted by IAA. IAA promotes Cd accumulation in plant shoots by enhancing the accumulation of carotenoids and proline in S. nigrum and maintaining a high leaf SOD activity in S. melongena.

    更新日期:2020-01-22
  • Pulmonary oxidative stress in wild bats exposed to coal dust: A model to evaluate the impact of coal mining on health
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-21
    Giulia dos Santos Pedroso-Fidelis; Hémelin Resende Farias; Gustavo Antunes Mastella; Liana Appel Boufleur-Niekraszewicz; Johnny Ferraz Dias; Marcio Correa Alves; Paulo Cesar Lock Silveira; Renata Tiscoski Nesi; Fernando Carvalho; Jairo José Zocche; Ricardo Aurino Pinho
    更新日期:2020-01-22
  • Spinal cord neurodegeneration after inorganic mercury long-term exposure in adult rats: Ultrastructural, proteomic and biochemical damages associated with reduced neuronal density
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-18
    Márcio Gonçalves Corrêa; Leonardo Oliveira Bittencourt; Priscila Cunha Nascimento; Railson Oliveira Ferreira; Walessa Alana Bragança Aragão; Marcia Cristina Freitas Silva; Walace Gomes-Leal; Mileni Silva Fernandes; Aline Dionizio; Marília Rabelo Buzalaf; Maria Elena Crespo-Lopez; Rafael Rodrigues Lima

    Mercury chloride (HgCl2) is a chemical pollutant widely found in the environment. This form of mercury is able to promote several damages to the Central Nervous System (CNS), however the effects of HgCl2 on the spinal cord, an important pathway for the communication between the CNS and the periphery, are still poorly understood. The aim of this work was to investigate the effects of HgCl2 exposure on spinal cord of adult rats. For this, animals were exposed to a dose of 0.375 mg/kg/day, for 45 days. Then, they were euthanized, the spinal cord collected and we investigated the mercury concentrations in medullary parenchyma and the effects on oxidative biochemistry, proteomic profile and tissue structures. Our results showed that exposure to this metal promoted increased levels of Hg in the spinal cord, impaired oxidative biochemistry by triggering oxidative stress, mudulated antioxidant system proteins, energy metabolism and myelin structure; as well as caused disruption in the myelin sheath and reduction in neuronal density. Despite the low dose, we conclude that prolonged exposure to HgCl2 triggers biochemical changes and modulates the expression of several proteins, resulting in damage to the myelin sheath and reduced neuronal density in the spinal cord.

    更新日期:2020-01-21
  • Biotransformation and toxicity effect of monoanthraquinone dyes during Bjerkandera adusta CCBAS 930 cultures
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-20
    K. Rybczyńska-Tkaczyk; T. Korniłłowicz-Kowalska; K.A. Szychowski; J. Gmiński
    更新日期:2020-01-21
  • Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-21
    Ziquan Wang; Haixia Tian; Mei Lei; Mallavarapu Megharaj; Xiangping Tan; Fang Wang; Hanzhong Jia; Wenxiang He

    Information on the kinetic characteristics of soil enzymes under long-term arsenic (As) pollution in field soils is scarce. We investigated Michaelis-Menten kinetic properties of four soil enzymes including β-glucosidase (BG), acid phosphatase (ACP), alkaline phosphatase (ALP), and dehydrogenase (DHA) in field soils contaminated by As resulting from long-term realgar mining activity. The kinetic parameters, namely the maximum reaction velocity (Vmax), enzyme-substrate affinity (Km) and catalytic efficiency (Vmax/Km) were calculated. Results revealed that the enzyme kinetic characteristics varied in soils and were significantly influenced by total nitrogen (N) and total As, which explained 31.8% and 30.7% of the variance in enzyme kinetics respectively. Enzyme pools (Vmax) and catalytic efficiency (Vmax/Km) of BG, ACP and DHA decreased with elevated As pollution, while the enzyme affinity for substrate (Km) was less affected. Redundancy analysis and stepwise regression suggested that the adverse influence of As on enzyme kinetics may offset or weakened by soil total N and soil organic matter (SOM). Concentration-response fitting revealed that the specific kinetic parameters expressed as the absolute enzyme kinetic parameters multiplied by normalized soil total N and SOM were more relevant than the absolute ones to soil total As. The arsenic ecological dose values that cause 10% decrease (ED10) in the specific enzyme kinetics were 20–49 mg kg−1, with a mean value of 35 mg kg−1, indicating a practical range of threshold for As contamination at field level. This study concluded that soil enzymes exhibited functional adaptation to long-term As stress mainly through the reduction of enzyme pools (Vmax) or maintenance of enzyme-substrate affinity (Km). Further, this study demonstrates that the specific enzyme kinetics are the better indicators of As ecotoxicity at field-scale compared with the absolute enzyme parameters.

    更新日期:2020-01-21
  • Acid treated biochar enhances cadmium tolerance by restricting its uptake and improving physio-chemical attributes in quinoa (Chenopodium quinoa Willd.)
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-18
    Muhammad Asif Naeem; Arslan Shabbir; Muhammad Amjad; Ghulam Abbas; Muhammad Imran; Behzad Murtaza; Muhammad Tahir; Amna Ahmad

    Heavy metals contamination of soil especially with cadmium (Cd) is a serious environmental concern in the current industrial era. Biochar serves as an excellent ameliorating agent depending upon its properties and application rates. In the pot scale study, effect of acid treated (AWSB) and untreated wheat straw biochar (WSB) was studied on physiology, grain yield, Cd accumulation, and tolerance of quinoa with possible health risks. Different levels of Cd (0, 25, 50 and 75 mg kg−1), AWSB and WSB (1% and 2% (w/w)) were applied in soil. Accumulation of Cd in control plant tissues led to oxidative stress which was shown in terms of increased lipid peroxidation. While biochar application relieved the oxidative damage as confirmed by the low production of H2O2 and TBARS contents. Application of AWSB improved plant growth, pigment contents and gas exchange attributes by limiting the accumulation of Cd in root, shoot and grain of quinoa. Results revealed a significant improvement in the activity of superoxide (SOD), catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD) with biochar at elevated levels of Cd in soil. Target Hazard Quotient (THQ) remained < 1 in the quinoa grains with WSB and AWSB under Cd stress. These results revealed that AWSB most effectively alleviated Cd toxicity in quinoa thereby decreasing Cd accumulation and regulation of Cd induced oxidative stress triggered by the antioxidant enzymatic system.

    更新日期:2020-01-21
  • Analysis of model PM2.5-induced inflammation and cytotoxicity by the combination of a virtual carbon nanoparticle library and computational modeling
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-20
    Guohong Liu; Xiliang Yan; Alexander Sedykh; Xiujiao Pan; Xiaoli Zhao; Bing Yan; Hao Zhu
    更新日期:2020-01-21
  • Heterologous expression of the tetracycline resistance gene tetX to enhance degradability and safety in doxycycline degradation
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-20
    Xin Wen; Jielan Huang; Junchao Cao; Jiangran Xu; Jiandui Mi; Yan Wang; Baohua Ma; Yongde Zou; Xindi Liao; Juan Boo Liang; Yinbao Wu
    更新日期:2020-01-21
  • Characterization and source identification of PM2.5-bound polycyclic aromatic hydrocarbons in urban, suburban, and rural ambient air, central China during summer harvest
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-20
    Xinli Xing; Zhanle Chen; Qian Tian; Yao Mao; Weijie Liu; Mingming Shi; Cheng Cheng; Tianpeng Hu; Gehao Zhu; Ying Li; Huang Zheng; Jiaquan Zhang; Shaofei Kong; Shihua Qi

    Characterization and source identification of PM2.5–bound polycyclic aromatic hydrocarbons (PAHs) are conducted in urban Wuhan (WH), suburban Pingdingshan (PDS), and rural Suizhou (SZ) in China during summer harvest. This study analyzes 16 priority PAHs with 38 PM.2.5 samples in June. PAHs had similar physical-chemical properties like polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs), which had been listed as Priority Pollutants. The concentration and detection frequency of OCPs and PCBs were considerably lower than those of PAHs in PM2.5. Results indicate that PDS adjoining the highway has the highest PM2.5–bound PAHs. SZ possesses the lowest concentration of PAHs. Principal component analysis and multivariate linear regression model and molecular diagnostic ratio distinguish the sources. Vehicle emissions and coal combustion are extracted in three sites, while the source of PDS also includes gas combustion. SZ was affected by gas combustion and petroleum. The potential source contribution function and the concentration-weighted trajectory track the potential pollution area. The sampling places might be affected by the local sources and short distance transmission cannot be neglected. The incremental lifetime cancer risks (ILCRs) model evaluates the exposure risk of PAHs. According to the ILCR model, WH and PDS are exposed to harmful PAHs. By contrast, SZ is a substantially safe place.

    更新日期:2020-01-21
  • Bacterial and archaeal compositions and influencing factors in soils under different submergence time in a mercury-sensitive reservoir
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-20
    Hongxia Du; Tao Sun; Dingyong Wang; Ma Ming

    Soils in the water-level-fluctuating zone (WLFZ) of Three Gorges Reservoir (TGR) inundated by water for different periods of time are confirmed to have disparate characteristics to mercury (Hg), and thus it is of great significance to further investigate microbial compositions and influencing factors. The objective of this study was to compare bacterial and archaeal richness, α-diversities and compositions, as well as affecting variables, especially Hg concentrations, among soils under different submergence time—SI (inundated soil), SS (semi-inundated soil), SN(non-inundated soil) and SSe (sediment)—based on high throughput sequencing. Results showed that sediment had significantly higher bacterial and archaeal richness and α-diversities than the other soil types. Anaerolinea and Aeromonas, as well as Altiarchaeales, Nitrosoarchaeum, and Methanosarta were dominant in SSe, while sharply decreasing in the other soil types, with significant difference among groups. An unclassified genus in SCG critically predominating in SI, SS and SN, drastically reduced in SSe, with extremely significant difference among groups. Bathyarchaeota and Nitrososphaera, both dominating in SSe, decreased dramatically and almost vanished in SI and SN. All the variables except pH posed a significant positive effect on bacterial and archaeal compositions in SSe, while opposite effect in the other three soil types. MeHg and THg concentrations had relatively weaker effects on microbial compositions comparing to variables like NH4+, CEC, OM and SO42+.

    更新日期:2020-01-21
  • Interactive effects of roxithromycin and freshwater microalgae, Chlorella pyrenoidosa: Toxicity and removal mechanism
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-17
    Jiping Li; Zhongfang Min; Wei Li; Lijie Xu; Jiangang Han; Pingping Li

    Roxithromycin (ROX) has received increasing concern due to its large usage, ubiquitous detection in environment and high ecotoxicology risk. This study investigated the acute and chronic effects of ROX on the growth, chlorophyll, antioxidant enzymes, and malonaldehyde (MDA) content of Chlorella pyrenoidosa, as well as the removal mechanism of ROX during microalgae cultivation. The calculated 96 h median effective concentration of ROX on yield (EyC50) and specific growth rate (ErC50) of C. pyrenoidosa was 0.81 and 2.87 mg/L, respectively. After 96 h exposure, 1.0 ~ 2.0 mg/L of ROX significantly inhibited the synthesis of chlorophyll and promoted the activities of SOD and CAT (p < 0.05). The MDA content increased with the ROX concentration increasing from 0.5 ~ 1.0 mg/L, and then decreased to 105.76% of the control exposure to 2.0 mg/L ROX, demonstrating the oxidative damage could be moderated by the upregulation of SOD and CAT activities. During the 21 d chronic exposure, low concentration of ROX (0.1 and 0.25 mg/L) showed no significant effect on the growth and chlorophyll content of algae during the first 14 d, but significantly inhibited the growth of algae and the synthesis of chlorophyll at 21 d (p < 0.05 or p < 0.01). 1.0 mg/L ROX significantly inhibited the growth of microalgae during 3 ~ 21 d and the synthesis of chlorophyll at 7 ~ 21 d. High concentration and long-term exposure of low concentration of ROX caused the SOD and CAT activities and MDA content to increase, demonstrating a higher level of oxidative damage of microalgae. During the first 14 d, abiotic removal of ROX played a more important role, contributing about 12.21% ~ 21.37% of ROX removal. After 14 d, the biodegradation of ROX by C. pyrenoidosa gradually became a more important removal mechanism, contributing about 45.99% ~ 53.30% of ROX removal at 21 d. Bio-adsorption and bioaccumulation both played minor roles in the removal of ROX during algae cultivation.

    更新日期:2020-01-17
  • Degradation of prosulfocarb by hydroxyl radicals in gas and aqueous phase: Mechanisms, kinetics and toxicity
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-16
    Xiaofei Bo; Jianfei Sun; Qiong Mei; Bo Wei; Zexiu An; Dandan Han; Zhiqiang Li; Ju Xie; Jinhua Zhan; Maoxia He
    更新日期:2020-01-17
  • 更新日期:2020-01-17
  • The bioaccessibility and fractionation of arsenic in anoxic soils as a function of stabilization using low-cost Fe/Al-based materials: A long-term experiment
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-17
    Qinxuan Hou; Dongya Han; Ying Zhang; Mei Han; Guanxing Huang; Liquan Xiao

    Arsenic (As)-contaminated soils occur widely worldwide. In the present study, three low-cost Fe/Al-based materials, including red soil (RS), sponge iron filter (SIF) and Al-based water treatment sludge (WTS), were applied as amendments to remediate As-contaminated soils under anoxic conditions. After 180 d of incubation, the proportion of the sum of nonspecifically absorbed As (F1) and specifically absorbed As (F2) to the total As was reduced by 6%, 52% and 13% with 5% of RS, SIF and WTS addition, respectively, compared to the control soil (31%). The results showed that among the three amendments, SIF was the most effective at decreasing As bioaccessibility in soils. Compared with RS and WTS, SIF intensified the decrease of labile fractions and the increase of unlabile fractions, and the redistribution of the amorphous oxide-bound fraction (F3) and crystalline hydrous oxide-bound fraction (F4) occurred in the SIF-amended soil. Moreover, the As stabilization processes were divided into two stages in the control and RS-amended soil, while the processes were divided into three stages in both SIF- and WTS-treated soil. The As stabilization processes in all treated soils were characterized by the transformation of labile fractions into more immobilizable fractions, except for F4 transforming into F3 in the first stage in SIF-amended soil. Correspondingly, inner-surface complexation and occlusion within Fe/Al hydroxides were the common driving mechanisms for the transformation of As fractions. Therefore, taking into consideration the results of this study, SIF could be a more promising alternative than the other two materials to passivate As in anoxic soils.

    更新日期:2020-01-17
  • Characterization of tetracycline effects on microbial community, antibiotic resistance genes and antibiotic resistance of Aeromonas spp. in gut of goldfish Carassius auratus Linnaeus
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-17
    Jia Jia; Mengqian Cheng; Xue Xue; Yongjing Guan; Zaizhao Wang

    The gut of aquatic animals was a significant niche for dissemination of antibiotic resistance genes (ARGs) and direct response of living conditions. In this study, the gut microbiota of goldfish Carassius auratus Linnaeus was sampled at 7 days and 21 days after treatment with tetracycline at 0.285 and 2.85 μg L−1 to investigate the influences on the microbial structure and antibiotic resistance. The proportion of tetracycline resistance bacteria was 1.02% in the control group, while increased to 23.00%, 38.43%, 62.05% in groups of high concentration for 7 days (H7), low concentration for 21 days (L21) and high concentration for 21 days (H21), respectively. Compared to the control group, the diversity of isolated Aeromonas spp. was decreased in the treatment groups and the minimal inhibitory concentration (MIC) of resistant isolates was enhanced from 32 to 256 μg mL−1 with the treatment of tetracycline in time- and dose-dependent manners. Furthermore, the abundance of most genes was increased in treatment groups and efflux genes mainly responded to the stress of tetracycline with an average level of 1.0 × 10−2. After treatment with tetracycline, the predominant species were changed both at phylum and genus levels. The present study explored the impact of tetracycline on gut microbiota of goldfish at environmentally realistic concentrations for the first time and our findings will provide a reference for characterizing the microbiome of fish in the natural environment.

    更新日期:2020-01-17
  • Uptake of atenolol, carbamazepine and triclosan by crops irrigated with reclaimed water in a Mediterranean scenario
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-17
    Eulalia María Beltrán; María Victoria Pablos; Carlos Fernández Torija; Miguel Ángel Porcel; Miguel González-Doncel
    更新日期:2020-01-17
  • Increasing soil moisture faciliates the outcomes of exogenous sulfate rather than element sulfur in reducing cadmium accumulation in rice (Oryza sativa L.)
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-17
    Tong-Tong Liu; Dao-You Huang; Qi-Hong Zhu; Jian-Li Zhou; Quan Zhang; Han-Hua Zhu; Chao Xu
    更新日期:2020-01-17
  • Exogenous salicylic acid ameliorates heat stress-induced damages and improves growth and photosynthetic efficiency in alfalfa (Medicago sativa L.)
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-16
    Misganaw Wassie; Weihong Zhang; Qiang Zhang; Kang Ji; Liwen Cao; Liang Chen

    Heat stress is found to be a detrimental factor for growth and development of alfalfa (Medicago sativa L.) which is tremendously invaluable forage due to its high feed value and yield potential. Salicylic acid (SA) has been reported to play a pivotal role in the regulation of plants biotic and abiotic stress response. However, the role of exogenous SA in protecting alfalfa from heat-induced damage has rarely been studied. In this study, four-week-old alfalfa seedlings were treated with 0.25 mM or 0.5 mM SA five days prior to high stress treatment (three day), and various growth and physiological traits were measured. The results showed that exogenous SA pretreatment could improve leaf morphology, plant height, biomass, chlorophyll content, and photosynthetic efficiency of alfalfa under heat stress. Meanwhile, SA could alleviate heat-induced membrane damage by reducing electrolyte leakage (EL) and malondialdehyde (MDA) content, and regulate the activities of antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD). The results revealed that exogenous SA application enhanced alfalfa heat tolerance by modulating various morphological and physiological characteristics under heat stress, with more prominent effect at lower concentration (0.25 mM). Overall, this study provides fundamental insights into the SA-mediated physiological adaptation of alfalfa plants to heat stress, which could have useful implication in managing other plants which are suffering global warming.

    更新日期:2020-01-16
  • Toxicokinetics and bioaccumulation characteristics of bisphenol analogues in common carp (Cyprinus carpio)
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-15
    Qiang Wang; Meng Chen; Liwen Qiang; Wei Wu; Jing Yang; Lingyan Zhu
    更新日期:2020-01-16
  • In-vitro evaluation of rice straw biochars’ effect on bispyribac-sodium dissipation and microbial activity in soil
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-16
    Neha Sharma; Pervinder Kaur; Deepali Jain; Makhan Singh Bhullar
    更新日期:2020-01-16
  • Highly biodegradable fluoroquinolone derivatives designed using the 3D-QSAR model and biodegradation pathways analysis
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-16
    Yilin Hou; Yuanyuan Zhao; Qing Li; Yu Li

    A three-dimensional quantitative structure–activity relationship (3D-QSAR) model was established based on molecular structures and docking scores (representing the biodegradability); the scores were obtained for 23 fluoroquinolones (FQs) and the oxidoreductase (PDB ID: 1YZP) of Phanerochaete chrysosporium in the aerobic process of municipal wastewater treatment plants. In the Comparative Molecular Field Analysis (CoMFA) model, q2 was 0.516 and r2pred was 0.727, which showed that the model was reliable and robust. The modification information obtained by the contour maps showed that introducing electronegative, bulky or electropositive groups at different active sites could increase the biodegradability of fluoroquinolone derivatives. Using levofloxacin (LEV) as a modified molecule, 35 fluoroquinolone derivatives with higher biodegradability than LEV were designed. After the evaluation of genotoxicity, bioconcentration and photodegradation, Derivative-15, with higher biodegradability (increased by 27.85%), higher genotoxicity, higher photodegradation and lower bioconcentration, was identified as the most environmentally friendly fluoroquinolone derivative. The 2D-QSAR model of FQ biodegradability was established through the quantization parameters, and q+ was identified as the main parameter affecting the biodegradability of FQs through sensitivity analysis. In addition, the docking results of LEV and Derivative-15 with the oxidoreductase in P. chrysosporium showed that the electrostatic field force between Derivative-15 and the amino acid residues promoted the binding of the donor to the receptor protein, thereby increasing the biodegradability of Derivative-15. Additionally, molecular dynamics simulations revealed that the enhancement of the electrostatic field force with Derivative-15 could promote the binding of the ligand to the receptor, which was basically consistent with the conclusion of molecular docking. Finally, the three microbial degradation pathways of LEV and Derivative-15 were also proposed. The total energy barrier value of the pathway with the lowest total energy barrier of biodegradation was reduced by 32.07%, which was basically consistent with the enhancement of biodegradability of Derivative-15.

    更新日期:2020-01-16
  • Differences in distribution of functional microorganism at DNA and cDNA levels in cow manure composting
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-15
    Qingxin Meng; Yue Han; Haifeng Zhu; Wei Yang; Ayodeji Bello; Liting Deng; Xin Jiang; Xiaotong Wu; Siyuan Sheng; Yingying Xu; Xiuhong Xu

    Denitrification and nitrification processes are the two prominent pathways of nitrogen (N) transformation in composting matrix. This study explored the dynamics of denitrifying and nitrifying bacteria at different composting stages of cow manure and corn straw using functional gene sequencing at DNA and cDNA levels. Corresponding agreement among OTUs, NMDS, mental test and network analyses revealed that functional bacteria community compositions and responses to physicochemical factors were different at DNA and cDNA levels. Specifically, some OTUs were detected at the DNA level but were not observed at cDNA level, differences were also found in the distribution patterns of nitrifying and denitrifying bacteria communities at both levels. Furthermore, co-occurrence network analysis indicated that Pseudomonas, Paracoccus and Nitrosomonas were identified as the keystone OTUs at the DNA level, while Paracoccus, Agrobacterium and Nitrosospira were keystone OTUs at the cDNA level. Mantel test revealed that TN, C/N and moisture content significantly influenced both the denitrifying bacteria and ammonia-oxidizing bacteria (AOB) communities at the DNA level. NO3−-N, NH4+-N, TN, C/N, and moisture content only registered significant correlation with the nosZ-type denitrifiers and ammonia-oxidizing bacteria (AOB) communities at the cDNA level. Structural equation model (SEM) showed that TN, NH4+-N, and pH were direct and significantly influenced the gene abundance of denitrifying bacteria. Howbeit, TN, NH4+-N, and NO3−-N had significant direct effects on amoA gene abundance.

    更新日期:2020-01-15
  • Accumulation and effects of perfluoroalkyl substances in three hydroponically grown Salix L. species
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-14
    Nisha Sharma; Giuseppe Barion; Inisa Shrestha; Leonard Barnabas Ebinezer; Anna Rita Trentin; Teofilo Vamerali; Giustino Mezzalira; Antonio Masi; Rossella Ghisi
    更新日期:2020-01-15
  • Fish response of metal bioaccumulation to reduced toxic load on long-term contaminated lake Imandra
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-15
    Natalia A. Gashkina; Tatyana I. Moiseenko; Lubov P. Kudryavtseva

    The present study analysed the response of whitefish (Coregonus lavaretus L.) to reduced toxicity after the long-term contamination of subarctic Lake Imandra. High concentrations of Ni, Al, and Sr in fish organs and tissues were accompanied by nephrocalcinosis, scoliosis, and myopathy during the period of intense contamination. After reduction of the toxic impact on the lake, the accumulation of Cu, Al, Sr, Cr, Pb, and Hg in the kidney, which is the target organ for toxicity, was two-fold less and that of Cd was 10-fold less in whitefish from the contaminated part of the lake compared with those in whitefish from the non-contaminated parts of the lake. The ecological success of whitefish from the contaminated part of the lake was associated with the limited accumulation of metals in organs and tissues and a more favourable physiological state compared with whitefish from non-contaminated parts of the lake. Redistribution models were constructed for essential and non-essential metals in the fish liver and kidney depending on the physiological state of fish. The results revealed changes in Fe and Zn metabolism: an increase in Fe and a decrease in Zn accumulation in the liver with increasing stage of liver disease and decreasing blood haemoglobin concentration. Furthermore, under the sub-toxic (except for Cu) conditions of Lake Imandra, the strategy of adaptive fish response is to preserve and maintain acid–base regulation system.

    更新日期:2020-01-15
  • Acute toxicity assessment of arsenic, chromium and almix 20WP in Euphlyctis cyanophlyctis tadpoles
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-15
    Palas Samanta; Sandipan Pal; Aloke Kumar Mukherjee; Apurba Ratan Ghosh

    Heavy metals and herbicide are gaining serious environmental concern in aquatic toxicology due to its adverse effects on aquatic organisms especially amphibians. Accordingly, present study first time evaluated the acute toxicity of two heavy metals [arsenic (As3+) and chromium (Cr6+)] and a herbicide (Almix) to Indian skittering frog tadpole, Euphlyctis cyanophlyctis. The LC50 values of As, Cr and Almix for 24, 48, 72 and 96 h were 73.58, 56.31, 43.58 and 32.58 mg L−1; 326.68, 224.31, 171.92 and 141.99 mg L−1; and 1297.85, 1148.22, 1033.62 and 955.17 mg L−1, respectively. It also revealed the concentration- and time-dependent increased mortality rate under these toxicants. The safety concentrations (SC) of As, Cr and Almix to tadpoles were 3.26, 14.20 and 95.52 mg L−1, respectively. The findings disclosed that As is highly toxic to E. cyanophlyctis than Cr and Almix. Alkaline phosphatase (ALP) activity showed varied responses to exposed chemicals. In particularly, ALP activity reduced significantly for Cr treatment. Glutathione S-transferase (GST) activity in E. cyanophlyctis was significantly inhibited by As treatment (p < 0.05); however, GST activity was remain unchanged for Cr and Almix (p > 0.05). The As toxicity correlates positively with GST inhibition (r = 0.779, p < 0.01); contrarily, Cr and Almix revealed negative correlation with GST induction (r = −0.461 and −0.19, respectively; p > 0.05). This result indicated that GST play a crucial role for regulating the tadpole mortality and intoxication by As, Cr and Almix. Overall, our findings demonstrate the different levels of toxic sensitivity (adverse effects) under different toxicants on E. cyanophlyctis tadpoles. Finally, the present findings could be used as baseline information of toxicosis for metalloid, heavy metal and herbicide exposures in wild frog populations.

    更新日期:2020-01-15
  • A review on control factors of pyrolysis technology for plants containing heavy metals
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-14
    Zhongchuang Liu; Li-ao Wang; Hongyan Xiao; Xiaowei Guo; Oksana Urbanovich; Liubov Nagorskaya; Xiang Li
    更新日期:2020-01-15
  • A sensitive sensor based on MOFs derived nanoporous carbons for electrochemical detection of 4-aminophenol
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-15
    Sisi Chen; Runmin Huang; Jiao Zou; Dan Liao; Jingang Yu; Xinyu Jiang
    更新日期:2020-01-15
  • Effect of pH on the adsorption of arsenic(V) and antimony(V) by the black soil in three systems: Performance and mechanism
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-15
    Yurui Fan; Chunli Zheng; Hongxia Liu; Chi He; Zhenxing Shen; Tian C. Zhang
    更新日期:2020-01-15
  • ROS-mediated miR-21-5p regulates the proliferation and apoptosis of Cr(VI)-exposed L02 hepatocytes via targeting PDCD4
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-15
    Yujing Zhang; Yuanyuan Xiao; Yu Ma; Ningjuan Liang; Yuehui Liang; Chan Lu; Fang Xiao

    Although much has been determined about the molecular mechanisms of hexavalent chromium [Cr(VI)]-induced hepatotoxicity, more remains to be explored. In particular, explicit epigenetic alterations of microRNAs (miRNAs) which can negatively regulate mRNAs at post transcriptional level remain understudied. In the present study, cell apoptosis was determined using Annexin V/propidium iodide (PI) staining, while proliferative growth was analyzed by colony formation assay and proliferating cell nuclear antigen (PCNA) detection. miRNA microarray was performed to compare the global miRNAs expression patterns. miR-21-5p mimics (mi)/inhibitor (in), and PDCD4-siRNAs were transfected into L02 hepatocytes. Our results revealed that Cr(VI) induced apoptosis and inhibited proliferation in L02 hepatocytes via reactive oxygen species (ROS), the formation of which is closely related to mitochondrial damage, especially the inhibition of mitochondrial respiratory chain complex (MRCC). We also confirmed that ROS-mediated miR-21-5p inhibition participated in cell apoptosis and proliferative inhibition induced by Cr(VI). Furthermore, programmed cell death protein 4 (PDCD4), the up-regulation of which was related to ROS over-production, was predicted and verified as a target of miR-21-5p. Transcription factor PDCD4 silencing suppressed apoptosis and stimulated cell proliferation. In conclusion, from the perspective of epigenetics, the present study revealed that ROS-mediated miR-21-5p regulated the proliferation and apoptosis of Cr(VI)-exposed L02 hepatocytes via targeting PDCD4, which provided the new targets for molecular intervention and treatment of liver damage in Cr(VI)-exposed population.

    更新日期:2020-01-15
  • Associations between fine particulate matter constituents and daily cardiovascular mortality in Shanghai, China
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-15
    Cuiping Wang; Lipeng Hao; Cong Liu; Renjie Chen; Weidong Wang; Yichen Chen; Yining Yang; Xia Meng; Qingyan Fu; Zhekang Ying; Haidong Kan
    更新日期:2020-01-15
  • Health risk assessment and bioaccessibilities of heavy metals for children in soil and dust from urban parks and schools of Jiaozuo, China
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-15
    Qiao Han; Mingshi Wang; Jingli Cao; Chenlu Gui; Yanping Liu; Xiangdong He; Yuchuan He; Yang Liu
    更新日期:2020-01-15
  • Does nitrogen source influence cadmium distribution in Arabidopsis plants?
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-14
    Analía Vazquez; Laura Recalde; Andrea Cabrera; María Daniela Groppa; María Patricia Benavides

    The purpose of the present work was to study the effect of the nitrogen source (NO3- vs NH4+) on cadmium (Cd) uptake, translocation and partition and its associated toxicity in hydroponically-grown Arabidopsis plants. After a short growth period on a complete Hoagland nutrient solution, Arabidopsis seedlings continued in the same growth medium (NA) or were switched to NO3– (N) or NH4+ (A) as sole N sources and supplied with 2.5 μM Cd. Unrelated to the nitrogen source, Cd reached higher levels in roots than in leaves. However, when ammonium was the source of nitrogen, Cd accumulation in roots was lower than in N or NA medium and the metal translocation to the aerial part was restricted, reaching values 25%–35% below the levels observed in plants grown with N or NA. Cadmium negatively affected chlorophyll content and PSII quantum yield, independently of the nitrogen source, with the highest decrease (35%) under NA treatment. Proline content increased, either with NA, N or A supplied in the presence of Cd, whereas a rise in total anthocyanin content was clearly favored when ammonium was the source of nitrogen, with or without Cd. In leaves, while NIA1 and NIA2 expression was markedly reduced by Cd in the presence of N or NA, ammonium source slightly reduced NIA1 expression but greatly upregulated NIA2 expression upon Cd exposure. The decay in NR activity was independent of the nitrogen source when Cd was applied and this decay was accompanied by a great increase in NH4+ levels either with nitrates or ammonium in the medium in the presence of Cd. Only NIA1 was detected in roots and its expression, together with NR activity and nitrates levels, was the highest in N medium devoid of Cd. The possibility of reducing Cd health risks through nitrogen fertilization practices is discussed.

    更新日期:2020-01-14
  • 更新日期:2020-01-14
  • 更新日期:2020-01-14
  • Bioaccumulation, biodistribution,and depuration of 13C-labelled fullerenols in zebrafish through dietary exposure
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-11
    Qiuyue Shi; Han Zhang; Chenglong Wang; Hongyun Ren; Changzhou Yan; Xian Zhang; Xue-Ling Chang
    更新日期:2020-01-13
  • Laccase mediator system obtained from a marine spore exhibits decolorization potential in harsh environmental conditions
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-11
    Elaheh Asadi; Ali Makhdoumi; Ahmad Asoodeh

    Laccases play a significant role in remedying dye pollutants. Most of these enzymes are originated from terrestrial fungi and bacteria, thus they are not proper to be used in the environments with neutral/alkaline pH, or they may require laborious extraction/purification steps. These limitations can be solved using marine spore laccases through high stability and easy to use application. In the current study, laccase activity of the marine spore -forming Bacillus sp. KC2 was measured according to the guaiacol and syringaldazine oxidation. Abiotic stresses like pH of 6, temperature of 37 °C and 0.3 mM CuSO4 (in comparison with optimal sporulation conditions: pH of 8, temperature of 20 °C and 0.0 mM CuSO4) enhanced laccase formation in sporal coat. Maximum activity of enzyme was observed at 50 °C and pH 7, which did not change in the alkaline pH and temperature range of 20–70 °C. Results indicated ions, inhibitors and solvent stability of the enzyme and its activity were stimulated by Co2+, Mn2+, PMSF, acetone, acetonitrile, ethanol, and methanol. The spore laccase could decolorize synthetic dyes from various chemical groups including azo (acid orange, amaranth, trypan blue, congo red, and amido black), indigo (indigo carmine), thiazine (methylene blue, and toluidine blue), and triarylmethane (malachite green) with ABTS/syringaldazine mediators after 5 h. Degradation products were not toxic against Sorghum vulgare and Artemia salina model organisms. The enzyme mediator system showed high potentials for dye bioremediation over a wide range of harsh conditions.

    更新日期:2020-01-13
  • Investigating photo-driven arsenics’ behavior and their glucose metabolite toxicity by the typical metallic oxides in ambient PM2.5
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-11
    Na Zhu; Ying Li; Junheng Jiao; Yang Yun; Tingting Ku; Dong Liang; Nan Sang

    It is essential and challenged to understand the atmospheric arsenic pollution because it is much more complicated than in water and top-soil. Herein the different behavior of arsenic species firstly were discovered within the ambient PM2.5 collected during daytime and nighttime, winter and summer. The diurnal variation of arsenic species in PMs is significantly correlated with the presence of metallic oxides, specifically, ferrous, titanium and zinc oxides, which might play a key role in the process of the photo-oxidation of As(III) to As(V) with the meteorological parameters and regional factors excluded. Subsequently, the photo conversion of arsenite was detected on metal-loaded glass-fiber filters under visible light. The photo-generated superoxide radical was found to be predominantly responsible for the oxidation of As(III). In order to reveal toxicity differences induced by oxidation As(III), HepG2 cells were exposed to various arsenic mixture solution. We found that the antioxidant enzyme activities suppressed with increasing the As(III)/As(V) ratio in total, followed by the accumulation of intracellular ROS level. The glucose consumption and glycogen content also displayed an obvious reduction in insulin-stimulated cells. Compared to the expression levels of IRS-1, AKT and GLUT4, GLUT2 might be more vulnerable to arsenic exposure and lead to the abnormalities of glucose metabolism in HepG2 cells. Taken together, these findings clarify that the health risk posed by inhalation exposure to As-pollution air might be alleviated owing to the photo-driven conversion in presence of metal oxides.

    更新日期:2020-01-13
  • Human arsenic exposure and lung function impairment in coal-burning areas in Guizhou, China
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-08
    Wenjuan Wang; Qingling Wang; Zhonglan Zou; Fanyan Zheng; Aihua Zhang

    To evaluate the effect of coal-burning arsenic (As) exposure on lung function and the potential underlying mechanisms, a total of 217 As-exposed subjects and 75 reference subjects were recruited into this study. Hair arsenic (H–As), pulmonary function tests, and serum inflammatory markers CC16, SP-A, MMP-9, and TIMP-1 were evaluated. Residents from As-exposed areas showed higher H–As concentrations (median 0.25 μg/g) than subjects from the reference area (median 0.14 μg/g). Large reductions in lung function parameters were noted in the As-exposed group. A significant negative correlation was observed between H–As concentrations and lung function. Specifically, monotonic negative dose-response relationships were observed between H–As and FEV1(%), FEV1/FVC (%) and FEF75 (%) (all P < 0.05), while the associations between H–As and FVC (%), FEF25 (%), and FEF50 (%) were nonlinear (P for nonlinearity = 0.03, 0.001, 0.01, respectively). In addition, there was a direct positive relationship between H–As and the inflammatory response. Alterations in inflammatory biomarkers (CC16, SP-A, MMP-9, and MMP-9/TIMP-1) were significantly associated with As-induced lung function impairment. Thus, this population-based study revealed that As exposure has significant toxic effects on lung function and increased inflammation may occur during this toxic process. We provide scientific evidence for an As-induced alteration in inflammatory biomarkers and pulmonary damage in an As-exposed population. The results of this study can inform risk assessment and risk control processes in relation to human As exposure in coal-burning arsenicosis areas.

    更新日期:2020-01-09
  • Nitric oxide is involved in nano-titanium dioxide-induced activation of antioxidant defense system and accumulation of osmolytes under water-deficit stress in Vicia faba L.
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-09
    M. Nasir Khan; Mazen A. AlSolami; Riyadh A. Basahi; Manzer H. Siddiqui; Asma A. Al-Huqail; Zahid Khorshid Abbas; Zahid H. Siddiqui; Hayssam M. Ali; Faheema Khan
    更新日期:2020-01-09
  • 更新日期:2020-01-09
  • Exogenous jasmonic acid decreased Cu accumulation by alfalfa and improved its photosynthetic pigments and antioxidant system
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-09
    Huiping Dai; Shuhe Wei; Marta Pogrzeba; Szymon Rusinowski; Jacek Krzyżak; Genliang Jia

    Jasmonic acid (JA) is an important phytohormone, which among others may be involved in the regulation of plant accumulating heavy metal. This experiment was designed to explore the effects of exogenous JA on the responses of alfalfa to Cu stress (100 μM) in Hoagland solution. When 1, 5 or 10 mM JA was added to the treatment with Cu addition, Cu concentrations in roots and leaves of alfalfa were significantly decreased (p < 0.05) to some extents compared to the treatment without JA addition. The biomasses of roots and leaves of alfalfa in treatments of JA additions were significantly increased (p < 0.05) compared to the Cu stress treatment. Similarly, the concentrations of Chlorophyll, antioxidant enzyme activities, MDA and H2O2 were improved accordingly. But these factors of JA were not improved further when its concentration added in media was the highest (10 mM), indicating its improvement roles were limited. These results suggested that there were positive roles of exogenous JA on alfalfa decreased its Cu accumulation and toxicities might via reduced oxidative stress.

    更新日期:2020-01-09
  • Chlorophyll synthesis and the photoprotective mechanism in leaves of mulberry (Morus alba L.) seedlings under NaCl and NaHCO3 stress revealed by TMT-based proteomics analyses
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-08
    Zhang Huihui; Wang Yue; Li Xin; He Guoqiang; Che Yanhui; Teng Zhiyuan; Shao Jieyu; Xu Nan; Sun Guangyu

    Chlorophyll (Chl) and effective photoprotective mechanism are important prerequisites to ensure the photosynthetic function of plants under stress. In this study, the effects of 100 mmol L−1 NaCl and NaHCO3 stress on chlorophyll synthesis and photosynthetic function of mulberry seedlings were studied by physiological combined with proteomics technology. The results show that: NaCl stress had little effect on the expression of Chl synthesis related proteins, and there were no significant changes in Chl content and Chl a:b ratio. However, 13 of the 15 key proteins in the process of Chl synthesis were significantly decreased under NaHCO3 stress, and the contents of Chl a and Chl b were significantly decreased (especially Chl a). Although stomatal conductance (Gs) decreased significantly under NaCl stress, net photosynthetic rate (Pn), PSII maximum photochemical efficiency (Fv/Fm) and electron transfer rate (ETR) did not change significantly, but under NaHCO3 stress, not only Gs decreased significantly, PSII activity and photosynthetic carbon were the same. In the photoprotective mechanism under NaCl stress, NAD(P)H dehydrogenase (NDH)-dependent cyclic electron flow (CEF) enhanced, the expression of related proteins subunit, ndhH, ndhI, ndhK, and ndhM, the key enzyme of the xanthophyll cycle, violaxanthin de-epoxidase (VDE) were up-regulated, the ratio of (A + Z)/(V + A + Z) and non-photochemical quenching (NPQ) was increased. The expressions of proteins FTR and Fd-NiR were also significant up-regulated under NaCl stress, Fd-dependent ROS metabolism and nitrogen metabolism can effectively reduce the electronic pressure on Fd. Under NaHCO3 stress, the expressions of NDH-dependent CEF related proteins subunit (ndhH, ndhI, ndhK, ndhM and ndhN), VDE, ZE, FTR, Fd-NiR and Fd-GOGAT, were significant down-regulated, and ZE, CP26, ndhK, ndhM, Fd-NiR, Fd-GOGAT and FTR genes expression also significantly decreased, the photoprotective mechanism, like the xanthophyll cycle,CEF and Fd-dependent ROS metabolism and nitrogen metabolism might be damaged, resulting in the inhibition of PSII electron transfer and carbon assimilation in mulberry leaves under NaHCO3 stress.

    更新日期:2020-01-08
  • Genotypic variation for cadmium tolerance in common bean (Phaseolus vulgaris L.)
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-08
    Ramin Bahmani; Mahsa Modareszadeh; Mohammad reza Bihamta
    更新日期:2020-01-08
  • Development of pp-LFER and QSPR models for predicting the diffusion coefficients of hydrophobic organic compounds in LDPE
    Ecotox. Environ. Saf. (IF 4.527) Pub Date : 2020-01-08
    Tengyi Zhu; Yue Jiang; Haomiao Cheng; Rajendra Prasad Singh; Bipeng Yan
    更新日期:2020-01-08
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