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BitterDB database analysis plus cell stiffness screening identify flufenamic acid as the most potent TAS2R14-based relaxant of airway smooth muscle cells for therapeutic bronchodilation Theranostics (IF 12.4) Pub Date : 2024-2-17 Kai Ni, Bo Che, Rong Gu, Chunhong Wang, Hongyang Xu, Huiduo Li, Shiyan Cen, Mingzhi Luo, Linhong Deng
Rationale: Bitter taste receptors (TAS2Rs) are abundantly expressed in airway smooth muscle cells (ASMCs), which have been recognized as promising targets for bitter agonists to initiate relaxation and thereby prevent excessive airway constriction as the main characteristic of asthma. However, due to the current lack of tested safe and potent agonists functioning at low effective concentrations, there
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Mrc1+ macrophage-derived IGF1 mitigates crystal nephropathy by promoting renal tubule cell proliferation via the AKT/Rb signaling pathway Theranostics (IF 12.4) Pub Date : 2024-2-17 Linxi Huang, Wei Chen, Zhuojing Tan, Yunxiao Huang, Xinji Gu, Lantian Liu, Hongxia Zhang, Yihan Shi, Jiarong Ding, Chengjian Zheng, Zhiyong Guo, Bing Yu
Rationale: The present understanding of the cellular characteristics and communications in crystal nephropathy is limited. Here, molecular and cellular studies combined with single-cell RNA sequencing (scRNA-seq) were performed to investigate the changes in cell components and their interactions in glyoxylate-induced crystallized kidneys to provide promising treatments for crystal nephropathy./nMethods:
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Activatable prodrug for controlled release of an antimicrobial peptide via the proteases overexpressed in Candida albicans and Porphyromonas gingivalis Theranostics (IF 12.4) Pub Date : 2024-2-17 Lubna Amer, Maurice Retout, Jesse V. Jokerst
Candida albicans and Porphyromonas gingivalis are prevalent in the subgingival area where the frequency of fungal colonization increases with periodontal disease. Candida's transition to a pathogenic state and its interaction with P. gingivalis exacerbate periodontal disease severity. However, current treatments for these infections differ, and combined therapy remains unexplored. This work is based
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Terbium radionuclides for theranostic applications in nuclear medicine: from atom to bedside Theranostics (IF 12.4) Pub Date : 2024-2-17 Camille Van Laere, Michel Koole, Christophe M. Deroose, Michiel Van de Voorde, Kristof Baete, Thomas E. Cocolios, Charlotte Duchemin, Maarten Ooms, Frederik Cleeren
Terbium features four clinically interesting radionuclides for application in nuclear medicine: terbium-149, terbium-152, terbium-155, and terbium-161. Their identical chemical properties enable the synthesis of radiopharmaceuticals with the same pharmacokinetic character, while their distinctive decay characteristics make them valuable for both imaging and therapeutic applications. In particular,
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Focused ultrasound ablation of melanoma with boiling histotripsy yields abscopal tumor control and antigen-dependent dendritic cell activation Theranostics (IF 12.4) Pub Date : 2024-2-11 Eric A. Thim, Lydia E. Kitelinger, Fátima Rivera-Escalera, Alexander S. Mathew, Michael R. Elliott, Timothy N. J. Bullock, Richard J. Price
Background: Boiling histotripsy (BH), a mechanical focused ultrasound ablation strategy, can elicit intriguing signatures of anti-tumor immunity. However, the influence of BH on dendritic cell function is unknown, compromising our ability to optimally combine BH with immunotherapies to control metastatic disease./nMethods: BH was applied using a sparse scan (1 mm spacing between sonications) protocol
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Integrated electronic/fluidic microneedle system for glucose sensing and insulin delivery Theranostics (IF 12.4) Pub Date : 2024-2-11 Xinshuo Huang, Baoming Liang, Shuang Huang, Zhengjie Liu, Chuanjie Yao, Jingbo Yang, Shantao Zheng, Feifei Wu, Wan Yue, Ji Wang, Huijiuan Chen, Xi Xie
Background: Precise and dynamic blood glucose regulation is paramount for both diagnosing and managing diabetes. Continuous glucose monitoring (CGM) coupled with insulin pumps forms an artificial pancreas, enabling closed-loop control of blood glucose levels. Indeed, this integration necessitates advanced micro-nano fabrication techniques to miniaturize and combine sensing and delivery modules on a
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Cancer-associated fibroblasts reprogram cysteine metabolism to increase tumor resistance to ferroptosis in pancreatic cancer Theranostics (IF 12.4) Pub Date : 2024-2-11 Yuchao Zhu, Shuai Fang, Bolin Fan, Kaiwei Xu, Liu Xu, Linwei Wang, Lubin Zhu, Chunqu Chen, Ruoyu Wu, Jiajing Ni, Jianhua Wang
Background: Pancreatic ductal adenocarcinoma (PDAC) is an insidious, rapidly progressing malignancy of the gastrointestinal tract. Due to its dense fibrous stroma and complex tumor microenvironment, neither of which is sensitive to radiotherapy, pancreatic adenocarcinoma is one of the malignancies with the poorest prognosis. Therefore, detailed elucidation of the inhibitory microenvironment of PDAC
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Forskolin-driven conversion of human somatic cells into induced neurons through regulation of the cAMP-CREB1-JNK signaling Theranostics (IF 12.4) Pub Date : 2024-2-11 Guodong Wang, Dandan Zhang, Liangshan Qin, Quanhui Liu, Wenkui Tang, Mingxing Liu, Fan Xu, Fen Tang, Leping Cheng, Huiming Mo, Xiang Yuan, Zhiqiang Wang, Ben Huang
Human somatic cells can be reprogrammed into neuron cell fate through regulation of a single transcription factor or application of small molecule cocktails./nMethods: Here, we report that forskolin efficiently induces the conversion of human somatic cells into induced neurons (FiNs)./nResults: A large population of neuron-like phenotype cells was observed as early as 24-36 h post-induction. There
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The DNA-dependent protein kinase catalytic subunit exacerbates endotoxemia-induced myocardial microvascular injury by disrupting the MOTS-c/JNK pathway and inducing profilin-mediated lamellipodia degradation Theranostics (IF 12.4) Pub Date : 2024-2-4 Rongjun Zou, Wanting Shi, Xing Chang, Miao Zhang, Songtao Tan, Ruibing Li, Hao Zhou, Yukun Li, Ge Wang, Weihui Lv, Xiaoping Fan
Rationale: The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) promotes pathological mitochondrial fission during septic acute kidney injury. The mitochondrial open reading frame of the 12S rRNA type-c (MOTS-c) is a mitochondria-derived peptide that exhibits anti-inflammatory properties during cardiovascular illnesses. We explored whether endotoxemia-induced myocardial microvascular injury
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MAGL protects against renal fibrosis through inhibiting tubular cell lipotoxicity Theranostics (IF 12.4) Pub Date : 2024-2-4 Shan Zhou, Xian Ling, Jielin Zhu, Ye Liang, Qijian Feng, Chao Xie, Jiemei Li, Qiyan Chen, Shuangqin Chen, Jinhua Miao, Mengyao Zhang, Zhiru Li, Weiwei Shen, Xiaolong Li, Qinyu Wu, Xiaoxu Wang, Ruiyuan Liu, Cheng Wang, Fan Fan Hou, Yaozhong Kong, Youhua Liu, Lili Zhou
Rationale: Renal fibrosis, with no therapeutic approaches, is a common pathological feature in various chronic kidney diseases (CKD). Tubular cell injury plays a pivotal role in renal fibrosis. Commonly, injured tubular cells exhibit significant lipid accumulation. However, the underlying mechanisms remain poorly understood./nMethods: 2-arachidonoylglycerol (2-AG) levels in CKD patients and CKD model
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Global metabolomics revealed deviations from the metabolic aging clock in colorectal cancer patients Theranostics (IF 12.4) Pub Date : 2024-2-4 Long Zhang, Shaobo Mo, Xiurui Zhu, C. James Chou, Bo Jin, Zhi Han, James Schilling, Weili Liao, Sheeno Thyparambil, Ruben Y. Luo, John C. Whitin, Lu Tian, Seema Nagpal, Scott R. Ceresnak, Harvey J. Cohen, Doff B. McElhinney, Karl G. Sylvester, Yangming Gong, Chen Fu, Xuefeng B. Ling, Junjie Peng
Background: Markers of aging hold promise in the context of colorectal cancer (CRC) care. Utilizing high-resolution metabolomic profiling, we can unveil distinctive age-related patterns that have the potential to predict early CRC development. Our study aims to unearth a panel of aging markers and delve into the metabolomic alterations associated with aging and CRC./nMethods: We assembled a serum cohort
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20-HETE mediated TRPV1 activation drives allokinesis via MrgprA3+ neurons in chronic dermatitis Theranostics (IF 12.4) Pub Date : 2024-2-4 Guang Yu, Pei Liu, Xiaobao Huang, Mingxin Qi, Xue Li, Weimeng Feng, Erxin Shang, Yuan Zhou, Changming Wang, Yan Yang, Chan Zhu, Fang Wang, Zongxiang Tang, Jinao Duan
Rationale: Noxious stimuli are often perceived as itchy in patients with chronic dermatitis (CD); however, itch and pain mechanisms of CD are not known./nMethods: TRPV1 involvement in CD was analyzed using a SADBE induced CD-like mouse model, and several loss- and gain-of-function mouse models. Trigeminal TRPV1 channel and MrgprA3+ neuron functions were analyzed by calcium imaging and whole-cell patch-clamp
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Emerging role of extracellular vesicles in diabetic retinopathy Theranostics (IF 12.4) Pub Date : 2024-2-4 Junyan Zhu, Jin Huang, Yaoxiang Sun, Wenrong Xu, Hui Qian
Diabetic retinopathy (DR), a complex complication of diabetes mellitus (DM), is a leading cause of adult blindness. Hyperglycemia triggers DR, resulting in microvascular damage, glial apoptosis, and neuronal degeneration. Inflammation and oxidative stress play crucial roles during this process. Current clinical treatments for DR primarily target the advanced retinal disorder but offer limited benefits
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Development of CD46 targeted alpha theranostics in prostate cancer using 134Ce/225Ac-Macropa-PEG4-YS5 Theranostics (IF 12.4) Pub Date : 2024-1-27 Kondapa Naidu Bobba, Anil P. Bidkar, Anju Wadhwa, Niranjan Meher, Suchi Drona, Alexandre M. Sorlin, Scott Bidlingmaier, Li Zhang, David M. Wilson, Emily Chan, Nancy Y. Greenland, Rahul Aggarwal, Henry F. VanBrocklin, Jiang He, Jonathan Chou, Youngho Seo, Bin Liu, Robert R. Flavell
Rationale: 225Ac, a long-lived α-emitter with a half-life of 9.92 days, has garnered significant attention as a therapeutic radionuclide when coupled with monoclonal antibodies and other targeting vectors. Nevertheless, its clinical utility has been hampered by potential off-target toxicity, a lack of optimized chelators for 225Ac, and limitations in radiolabeling methods. In a prior study evaluating
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High-content stimulated Raman histology of human breast cancer Theranostics (IF 12.4) Pub Date : 2024-1-27 Hongli Ni, Chinmayee Prabhu Dessai, Haonan Lin, Wei Wang, Shaoxiong Chen, Yuhao Yuan, Xiaowei Ge, Jianpeng Ao, Nolan Vild, Ji-Xin Cheng
Histological examination is crucial for cancer diagnosis, however, the labor-intensive sample preparation involved in the histology impedes the speed of diagnosis. Recently developed two-color stimulated Raman histology could bypass the complex tissue processing to generates result close to hematoxylin and eosin staining, which is one of the golden standards in cancer histology. Yet, the underlying
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Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Theranostics (IF 12.4) Pub Date : 2024-1-27 Meng Gao, Tuo Zhang, Tengxiang Chen, Ziqi Chen, Zijian Zhu, Yang Wen, Shaogang Qin, Yibing Bao, Ting Zhao, Hengxing Li, Longping Liu, Ming Hao, Jianbin Wang, Fengchao Wang, Haibin Wang, Bo Zhou, Hua Zhang, Guoliang Xia, Chao Wang
Rationale: Premature ovarian insufficiency (POI) is an accelerated reduction in ovarian function inducing infertility. Folliculogenesis defects have been reported to trigger POI as a consequence of ovulation failure. However, the underlying mechanisms remain unclear due to the genetic complexity and heterogeneity of POI./nMethods: We used whole genome sequencing (WGS), conditional knockout mouse models
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Tripeptidyl peptidase II coordinates the homeostasis of calcium and lipids in the central nervous system and its depletion causes presenile dementia in female mice through calcium/lipid dyshomeostasis-induced autophagic degradation of CYP19A1 Theranostics (IF 12.4) Pub Date : 2024-1-27 Jin Zhao, Chengtong He, Xueyu Fan, Lin Wang, Liao Zhao, Hui Liu, Wujun Shen, Sanwei Jiang, Kaixuan Pei, Jingjing Gao, Yawei Qi, Yang Liu, Junqiang Zhao, Ruiling Zhang, Chengbiao Lu, Jia Tong, Jisen Huai
Rationale: Tripeptidyl peptidase II (TPP2) has been proven to be related to human immune and neurological diseases. It is generally considered as a cytosolic protein which forms the largest known protease complex in eukaryotic cells to operate mostly downstream of proteasomes for degradation of longer peptides. However, this canonical function of TPP2 cannot explain its role in a wide variety of biological
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Enhancing anti-tumor potential: low-intensity vibration suppresses osteosarcoma progression and augments MSCs' tumor-suppressive abilities Theranostics (IF 12.4) Pub Date : 2024-1-27 Xue Xiong, Qingji Huo, Kexin Li, Changpeng Cui, Chunyi Chang, Charles Park, BonHeon Ku, Chin-Suk Hong, HeeChang Lim, Pankita H. Pandya, M. Reza Saadatzadeh, Khadijeh Bijangi-Vishehsaraei, Chien-Chi Lin, Melissa A. Kacena, Karen E. Pollok, Andy Chen, Jing Liu, William R. Thompson, Xue-Lian Li, Bai-Yan Li, Hiroki Yokota
Rationale: Osteosarcoma (OS), a common malignant bone tumor, calls for the investigation of novel treatment strategies. Low-intensity vibration (LIV) presents itself as a promising option, given its potential to enhance bone health and decrease cancer susceptibility. This research delves into the effects of LIV on OS cells and mesenchymal stem cells (MSCs), with a primary focus on generating induced
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Re-analysis of single-cell transcriptomics reveals a critical role of macrophage-like smooth muscle cells in advanced atherosclerotic plaque Theranostics (IF 12.4) Pub Date : 2024-1-27 Xue Gong, Yunchang Liu, Huiying Liu, Nian Cao, Liping Zeng, Miao Tian, Chunyu Zeng, Yijie Hu, Runjun Zhang, Yundai Chen, Gengze Wu
Aims: Smooth muscle cell (SMC) remodeling poses a critical feature in the development and progression of atherosclerosis. Although fate mapping and in silicon approaches have expanded SMC phenotypes in atherosclerosis, it still remains elusive about the contributions of individual SMC phenotypes and molecular dynamics to advanced atherosclerotic plaque./nMethods: Using single-cell transcriptome, we
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Transfer RNA derived fragment, tRF-Glu-CTC, aggravates the development of neovascular age-related macular degeneration Theranostics (IF 12.4) Pub Date : 2024-1-27 Yu Liang, Lingjie Kong, Yuelu Zhang, Yihan Zhang, Mingsu Shi, Jiaqiu Huang, Hongyu Kong, Siyi Qi, Yunlong Yang, Jiaxu Hong, Meidong Zhu, Xiangjia Zhu, Xinghuai Sun, Shujie Zhang, Lianqun Wu, Chen Zhao
Rationale: Angiogenesis expedites tissue impairment in many diseases, including age-related macular degeneration (AMD), a leading cause of irreversible blindness in elderly. A substantial proportion of neovascular AMD patients, characterized by aberrant choroidal neovascularization (CNV), exhibit poor responses or adverse reactions to anti-VEGF therapy. Herein, we aimed to unveil the function of newly
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The mitochondria‒paraspeckle axis regulates the survival of transplanted stem cells under oxidative stress conditions Theranostics (IF 12.4) Pub Date : 2024-1-27 Meng Zhao, Shuyun Liu, Yizhuo Wang, Ke Lv, Peng Lou, Pingya Zhou, Jiaying Zhu, Lan Li, Jingqiu Cheng, Yanrong Lu, Jingping Liu
Rationale: Stem cell-based therapies have emerged as promising tools for tissue engineering and regenerative medicine, but their therapeutic efficacy is largely limited by the oxidative stress-induced loss of transplanted cells at injured tissue sites. To address this issue, we aimed to explore the underlying mechanism and protective strategy of ROS-induced MSC loss./nMethods: Changes in TFAM (mitochondrial
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Degraders in epigenetic therapy: PROTACs and beyond Theranostics (IF 12.4) Pub Date : 2024-1-27 Xing-Jie Dai, Shi-Kun Ji, Meng-Jie Fu, Gao-Zhi Liu, Hui-Min Liu, Shao-Peng Wang, Liang Shen, Ning Wang, Piet Herdewijn, Yi-Chao Zheng, Sai-Qi Wang, Xiao-Bing Chen
Epigenetics refers to the reversible process through which changes in gene expression occur without changing the nucleotide sequence of DNA. The process is currently gaining prominence as a pivotal objective in the treatment of cancers and other ailments. Numerous drugs that target epigenetic mechanisms have obtained approval from the Food and Drug Administration (FDA) for the therapeutic intervention
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Metal nanoparticle hybrid hydrogels: the state-of-the-art of combining hard and soft materials to promote wound healing Theranostics (IF 12.4) Pub Date : 2024-1-27 Yuxiang Wang, Mengya Zhang, Zhenzhen Yan, Shizhao Ji, Shichu Xiao, Jie Gao
Wounds represent a grave affliction that profoundly impacts human well-being. Establishing barriers, preventing infections, and providing a conducive microenvironment constitute the crux of wound therapy. Hydrogel, a polymer with an intricate three-dimensional lattice, serves as a potent tool in erecting physical barriers and nurturing an environment conducive to wound healing. This enables effective
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Therapeutic effects of a novel electrode for transcranial direct current stimulation in ischemic stroke mice Theranostics (IF 12.4) Pub Date : 2024-1-21 Da Hee Jung, Jae Ho Lee, Hong Ju Lee, Jang Woo Park, Young-Jin Jung, Hwa Kyoung Shin, Byung Tae Choi
Rationale: Non-invasive transcranial direct current stimulation (tDCS), a promising stimulation tool to modulate a wide range of brain disorders, has major limitations, such as poor cortical stimulation intensity and focality. We designed a novel electrode for tDCS by conjugating a needle to a conventional ring-based high-definition (HD) electrode to enhance cortical stimulation efficacy./nMethod:
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Efficient PD-L1 imaging of murine glioblastoma with FUS-aided immunoPET by leveraging FcRn-antibody interaction. Theranostics (IF 12.4) Pub Date : 2023-10-16 Céline Chevaleyre,Anthony Novell,Nicolas Tournier,Ambre Dauba,Steven Dubois,Dimitri Kereselidze,Erwan Selingue,Benoit Jego,Bernard Maillère,Benoit Larrat,Hervé Nozach,Charles Truillet
Rationale: The passage of antibodies through the blood-brain barrier (BBB) and the blood-tumoral barrier (BTB) is determinant not only to increase the immune checkpoint inhibitors efficacy but also to monitor prognostic and predictive biomarkers such as the programmed death ligand 1 (PD-L1) via immunoPET. Although the involvement of neonatal Fc receptor (FcRn) in antibody distribution has been demonstrated
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Tat-NTS peptide protects neurons against cerebral ischemia-reperfusion injury via ANXA1 SUMOylation in microglia. Theranostics (IF 12.4) Pub Date : 2023-10-16 Huijuan Zhou,Lulu Yan,Hezhou Huang,Xing Li,Qian Xia,Lu Zheng,Bin Shao,Qian Gao,Ning Sun,Jing Shi
Rationale: Recent studies indicate that microglial activation and the resulting inflammatory response could be potential targets of adjuvant therapy for ischemic stroke. Many studies have emphasized a well-established function of Annexin-A1 (ANXA1) in the immune system, including the regulation of microglial activation. Nevertheless, few therapeutic interventions targeting ANXA1 in microglia for ischemic
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Engineered oncolytic bacteria HCS1 exerts high immune stimulation and safety profiles for cancer therapy. Theranostics (IF 12.4) Pub Date : 2023-10-16 Yujie Sun,Yanxia Guo,Xiaoqing Liu,Jinling Liu,Honglai Sun,Zhongying Li,Min Wen,Sheng-Nan Jiang,Wenzhi Tan,Jin Hai Zheng
Background and rationale: Attenuated Salmonella typhimurium VNP20009 has been used to treat tumor-bearing mice and entered phase I clinical trials. However, its mild anticancer effect in clinical trials may be related to insufficient bacterial colonization and notable adverse effects with increasing dosages. Guanosine 5'-diphosphate-3'-diphosphate (ppGpp) synthesis-deficient Salmonella is an attenuated
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Nanobody-mediated SPECT/CT imaging reveals the spatiotemporal expression of programmed death-ligand 1 in response to a CD8+ T cell and iNKT cell activating mRNA vaccine. Theranostics (IF 12.4) Pub Date : 2023-10-09 Thomas Ertveldt,Sofie Meulewaeter,Yannick De Vlaeminck,Oscar Olarte,Katrijn Broos,Serge Van Calenbergh,Stephanie Bourgeois,Joke Deprez,Yves Heremans,Cleo Goyvaerts,Willem Staels,Stefaan De Smedt,Heleen Dewitte,Nick Devoogdt,Marleen Keyaerts,Rein Verbeke,Kurt Barbé,Ine Lentacker,Karine Breckpot
Rationale: Although promising responses are obtained in patients treated with immune checkpoint inhibitors targeting programmed death ligand 1 (PD-L1) and its receptor programmed death-1 (PD-1), only a fraction of patients benefits from this immunotherapy. Cancer vaccination may be an effective approach to improve the response to immune checkpoint inhibitors anti-PD-L1/PD-1 therapy. However, there
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Theranostics with photodynamic therapy for personalized medicine: to see and to treat. Theranostics (IF 12.4) Pub Date : 2023-10-09 Youchao Wang,Johannes Nikodemus Staudinger,Thomas L Mindt,Gilles Gasser
Photodynamic Therapy (PDT) is an approved treatment modality, which is presently receiving great attention due to its limited invasiveness, high selectivity and limited susceptibility to drug resistance. Another related research area currently expanding rapidly is the development of novel theranostic agents based on the combination of PDT with different imaging technologies, which allows for both therapy
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Engineering CAR-T cells for radiohapten capture in imaging and radioimmunotherapy applications. Theranostics (IF 12.4) Pub Date : 2023-10-08 Keifer Kurtz,Laura Eibler,Megan M Dacek,Lukas M Carter,Darren R Veach,Samantha Lovibond,Emma Reynaud,Sarah Qureshy,Michael R McDevitt,Christopher Bourne,Sebastien Monette,Blesida Punzalan,Shireen Khayat,Svena Verma,Adam L Kesner,Nai-Kong V Cheung,Heiko Schöder,Leah Gajecki,Sarah M Cheal,Steven M Larson,David A Scheinberg,Simone Krebs
Rationale: The in vivo dynamics of CAR-T cells remain incompletely understood. Novel methods are urgently needed to longitudinally monitor transferred cells non-invasively for biodistribution, functionality, proliferation, and persistence in vivo and for improving their cytotoxic potency in case of treatment failure. Methods: Here we engineered CD19 CAR-T cells ("Thor"-cells) to express a membrane-bound
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Piezo1 specific deletion in macrophage protects the progression of liver fibrosis in mice. Theranostics (IF 12.4) Pub Date : 2023-10-02 Shangfei Luo,Xiaoduo Zhao,Jintao Jiang,Bo Deng,Silin Liu,Honglin Xu,Qiaorui Tan,Yu'an Chen,Ziyan Zhang,Xianmei Pan,Rentao Wan,Xiaoting Chen,Youfen Yao,Jing Li
Background and Aims: Liver fibrosis is the common pathological pathway of chronic liver diseases and its mechanisms of which have not been fully declared. Macrophages play essential roles in progression of liver fibrosis partially by sensing abnormal mechanical signals. The aim of the study is to investigate the functions of macrophage Piezo1, a mechano-sensitive ion channel, in liver fibrosis. Approach
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Inhalation of acidic nanoparticles prevents doxorubicin cardiotoxicity through improvement of lysosomal function. Theranostics (IF 12.4) Pub Date : 2023-10-02 Yohan Santin,Karina Formoso,Fraha Haidar,Maria Del Pilar Oreja Fuentes,Florence Bourgailh,Nesrine Hifdi,Karim Hnia,Yosra Doghri,Jessica Resta,Camille Champigny,Séverine Lechevallier,Maximin Détrait,Grégoire Cousin,Malik Bisserier,Angelo Parini,Frank Lezoualc'h,Marc Verelst,Jeanne Mialet-Perez
Doxorubicin (Dox) is an effective anticancer molecule, but its clinical efficacy is limited by strong cardiotoxic side effects. Lysosomal dysfunction has recently been proposed as a new mechanism of Dox-induced cardiomyopathy. However, to date, there is a paucity of therapeutic approaches capable of restoring lysosomal acidification and function in the heart. Methods: We designed novel poly(lactic-co-glycolic
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Systemic delivery of glycosylated-PEG-masked oncolytic virus enhances targeting of antitumor immuno-virotherapy and modulates T and NK cell infiltration. Theranostics (IF 12.4) Pub Date : 2023-10-02 Yuzhi Liang,Bing Wang,Qingjing Chen,Xingyue Fu,Chenwei Jiang,Zhiwen Lin,Qiuyu Zhuang,Yongyi Zeng,Xiaolong Liu,Da Zhang
Rationale: Immuno-virotherapy has emerged as a promising approach for cancer treatment, as it directly and cytotoxically eliminates tumors with systemic immune stimulation. However, the clinical efficacy of this approach remains limited by inappropriate delivery routes, robust antiviral responses, and the tumor immunosuppressive microenvironment. Methods: To address these challenges, we propose a surface
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In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental glioma. Theranostics (IF 12.4) Pub Date : 2023-09-25 Jessica Hunger,Katharina Schregel,Berin Boztepe,Dennis Alexander Agardy,Verena Turco,Kianush Karimian-Jazi,Ina Weidenfeld,Yannik Streibel,Manuel Fischer,Volker Sturm,Rachel Santarella-Mellwig,Michael Kilian,Kristine Jähne,Katharina Sahm,Wolfgang Wick,Lukas Bunse,Sabine Heiland,Theresa Bunse,Martin Bendszus,Michael Platten,Michael O Breckwoldt
Rationale: Intrinsic brain tumors, such as gliomas are largely resistant to immunotherapies including immune checkpoint blockade. Adoptive cell therapies (ACT) including chimeric antigen receptor (CAR) or T cell receptor (TCR)-transgenic T cell therapy targeting glioma-associated antigens are an emerging field in glioma immunotherapy. However, imaging techniques for non-invasive monitoring of adoptively
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PET imaging of focused-ultrasound enhanced delivery of AAVs into the murine brain. Theranostics (IF 12.4) Pub Date : 2023-09-25 Javier Ajenjo,Jai Woong Seo,Josquin Foiret,Bo Wu,Marina Nura Raie,James Wang,Brett Zain Fite,Nisi Zhang,Rim Malek,Corinne Beinat,Noeen Malik,David Alexander Anders,Katherine W Ferrara
Rationale: Despite recent advances in the use of adeno-associated viruses (AAVs) as potential vehicles for genetic intervention of central and peripheral nervous system-associated disorders, gene therapy for the treatment of neuropathology in adults has not been approved to date. The currently FDA-approved AAV-vector based gene therapies rely on naturally occurring serotypes, such as AAV2 or AAV9,
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1α,25(OH)2D3 ameliorates insulin resistance by alleviating γδ T cell inflammation via enhancing fructose-1,6-bisphosphatase 1 expression. Theranostics (IF 12.4) Pub Date : 2023-09-25 Peng Li,Ke Li,Wenhui Yuan,Yuqi Xu,Ping Li,Ruan Wu,Jingru Han,Zhinan Yin,Ligong Lu,Yunfei Gao
Background: Chronic inflammation caused by immune cells is the central link between obesity and insulin resistance. Targeting the inflammatory process is a highly promising method for reversing systemic insulin resistance. Methods: Blood samples were prospectively collected from 68 patients with type 2 diabetes. C57BL/6J mice were fed either a high-fat diet (HFD) or normal chow (NC). We performed phenotypical
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Upregulated FOXM1 stimulates chondrocyte senescence in Acot12-/-Nudt7-/- double knockout mice. Theranostics (IF 12.4) Pub Date : 2023-09-25 Jinsoo Song,Ee Hyun Kim,Jun-Ho Yang,Donghyeon Kim,Akhmad Irhas Robby,Se-Ah Kim,Sung Young Park,Ji Hyun Ryu,Eun-Jung Jin
Rationale: One of the hallmarks of osteoarthritis (OA), the most common degenerative joint disease, is increased numbers of senescent chondrocytes. Targeting senescent chondrocytes or signaling mechanisms leading to senescence could be a promising new therapeutic approach for OA treatment. However, understanding the key targets and links between chondrocyte senescence and OA remains unclear. Methods:
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Fe(III)-Shikonin supramolecular nanomedicines as immunogenic cell death stimulants and multifunctional immunoadjuvants for tumor vaccination. Theranostics (IF 12.4) Pub Date : 2023-09-25 Wenjie Feng,Wanrui Shi,Yanqi Cui,Jiajun Xu,Shuwei Liu,Hang Gao,Shoujun Zhu,Yi Liu,Hao Zhang
Immunoadjuvants, as an indispensable component of tumor vaccines, can observably enhance the magnitude, breadth, and durability of antitumor immunity. However, current immunoadjuvants suffer from different issues such as weak immunogenicity, inadequate cellular internalization, poor circulation time, and mono-functional bioactivity. Methods: Herein, we construct Fe3+-Shikonin metal-phenolic networks
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Visualizing vasculature and its response to therapy in the tumor microenvironment. Theranostics (IF 12.4) Pub Date : 2023-09-25 Qiaoya Lin,Peter L Choyke,Noriko Sato
Tumor vasculature plays a critical role in the progression and metastasis of tumors, antitumor immunity, drug delivery, and resistance to therapies. The morphological and functional changes of tumor vasculature in response to therapy take place in a spatiotemporal-dependent manner, which can be predictive of treatment outcomes. Dynamic monitoring of intratumor vasculature contributes to an improved
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The role of radiolabeling in BNCT tracers for enhanced dosimetry and treatment planning. Theranostics (IF 12.4) Pub Date : 2023-09-25 Sajid Mushtaq,Park Ji Ae,Jung Young Kim,Kyo Chul Lee,Kwang Il Kim
Positron emission tomography (PET) and single photon emission computed tomography (SPECT) are potent technologies for non-invasive imaging of pharmacological and biochemical processes in both preclinical and advanced clinical research settings. In the field of radiation therapy, boron neutron capture therapy (BNCT) stands out because it harnesses biological mechanisms to precisely target tumor cells
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Peptide-drug co-assembling: A potent armament against cancer. Theranostics (IF 12.4) Pub Date : 2023-09-25 Can Wu,Manman Wang,Jinpan Sun,Yongyan Jia,Xiali Zhu,Gaizhi Liu,Yanhui Zhu,Yanbin Guan,Zhenqiang Zhang,Xin Pang
Cancer is still one of the major problems threatening human health and the therapeutical efficacies of available treatment choices are often rather low. Due to their favorable biocompatibility, simplicity of modification, and improved therapeutic efficacy, peptide-based self-assembled delivery systems have undergone significant evolution. Physical encapsulation and covalent conjugation are two common
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Photocurable injectable Janus hydrogel with minimally invasive delivery for all-in-one treatment of gastric perforations and postoperative adhesions. Theranostics (IF 12.4) Pub Date : 2023-09-25 Xiaoqi Wu,Zihan Wang,Jie Xu,Liu Yu,Maoyu Qin,Jianfeng Li,Sitian Liu,Weihan Zheng,Zeyu Li,Jun Ouyang,Yanbing Li,Guoxin Li,Ling Wang,Wenhua Huang,Yaobin Wu
Background: Surgical sutures for sealing gastric perforations (GP) are associated with severe inflammation and postoperative adhesions. Hydrogel bioadhesives offer a potential alternative for sutureless repair of GP; however, their application in minimally invasive surgery is limited due to their prefabricated patch-form, lacking in situ gelation capability. In this study, we emphasized an all-in-one
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Tet1 deficiency exacerbates oxidative stress in acute kidney injury by regulating superoxide dismutase. Theranostics (IF 12.4) Pub Date : 2023-09-25 Yu Fan,Yangmian Yuan,Mingrui Xiong,Muchuan Jin,Donge Zhang,Dong Yang,Chengyu Liu,Robert B Petersen,Kun Huang,Anlin Peng,Ling Zheng
Rationale: Increased methylation of key genes has been observed in kidney diseases, suggesting that the ten-eleven translocation (Tet) methyl-cytosine dioxygenase family as well as 5mC oxidation may play important roles. As a member of the Tet family, the role of Tet1 in acute kidney injury (AKI) remains unclear. Methods: Tet1 knockout mice, with or without tempol treatment, a scavenger of reactive
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Universal theranostic CRISPR/Cas13a RNA-editing system for glioma. Theranostics (IF 12.4) Pub Date : 2023-09-25 Ye Wu,Yunfei Wang,Junhu Zhou,Jianhao Wang,Qi Zhan,Qixue Wang,Eryan Yang,Weili Jin,Fei Tong,Jixing Zhao,Biao Hong,Junrui Liu,Chunsheng Kang
Background: The CRISPR/Cas13a system offers the advantages of rapidity, precision, high sensitivity, and programmability as a molecular diagnostic tool for critical illnesses. One of the salient features of CRISPR/Cas13a-based bioassays is its ability to recognize and cleave the target RNA specifically. Simple and efficient approaches for RNA manipulation would enrich our knowledge of disease-linked
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FN1 mRNA 3'-UTR supersedes traditional fibronectin 1 in facilitating the invasion and metastasis of gastric cancer through the FN1 3'-UTR-let-7i-5p-THBS1 axis. Theranostics (IF 12.4) Pub Date : 2023-09-25 Siwei Pan,Jiaming Zhu,Pengfei Liu,Qiaochu Wei,Siyu Zhang,Wen An,Yuxin Tong,Zhenguo Cheng,Funan Liu
Background: Current clinical treatments for gastric cancer (GC), particularly advanced GC, lack infallible therapeutic targets. The 3'-untranslated region (3'-UTR) has attracted increasing attention as a drug target. Methods: In vitro and in vivo experiments were conducted to determine the function of FN1 3'-UTR and FN1 protein in invasion and metastasis. RNA pull-down assay and high-throughput sequencing
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Targeted elimination of senescent cells by engineered extracellular vesicles attenuates atherosclerosis in ApoE-/- mice with minimal side effects. Theranostics (IF 12.4) Pub Date : 2023-09-18 Liang Zhang,Chen Wang,Wei Hu,Te Bu,Wenqi Sun,Tian Zhou,Shuo Qiu,Mengying Wei,Helin Xing,Zhelong Li,Guodong Yang,Lijun Yuan
Senescent cells in plaques emerge as a detrimental factor for atherosclerosis (AS), for which targeted senolysis might be a promising therapeutic strategy. The development of safe and efficient senolytics for senescent cell eradication by targeted delivery is greatly needed. Methods: Pro-apoptotic intelligent Bax (iBax)-overexpressing plasmid was constructed by molecular cloning, in which Bax CDS was
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A dendritic/tumor fusion cell vaccine enhances efficacy of nanobody-based CAR-T cells against solid tumor. Theranostics (IF 12.4) Pub Date : 2023-09-18 Shuyang Sun,Ziqiang Ding,Li Gao,Bruce D Hammock,Xianing Huang,Zhi Ping Xu,Xuan Wang,Qihong Cheng,Fengzhen Mo,Wei Shi,Shenxia Xie,Aiqun Liu,Haixia Li,Xiaomei Yang,Xiaoling Lu
Background: Chimeric antigen receptor (CAR) T-cell therapy is practical in treating cancers of hematopoietic origin, but of that in solid tumors compromises efficacy for the loss of the antigen recognized by the CAR. However, dendritic cell (DC)/tumor fusion vaccines present a spectrum of known or unknown tumor antigens to stimulate T cell expansion and enhanced T cell response. Developing a new strategy
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Discovery of a novel NAMPT inhibitor that selectively targets NAPRT-deficient EMT-subtype cancer cells and alleviates chemotherapy-induced peripheral neuropathy. Theranostics (IF 12.4) Pub Date : 2023-09-11 Minjee Kim,Hyeyoung Kim,Bu-Gyeong Kang,Jooyoung Lee,Taegun Kim,Hwanho Lee,Jane Jung,Myung Joon Oh,Seungyoon Seo,Myung-Jeom Ryu,Yeojin Sung,Yunji Lee,Jeonghun Yeom,Gyoonhee Han,Sun-Shin Cha,Hosung Jung,Hyun Seok Kim
Background: Exploiting synthetic lethality (SL) relationships between protein pairs has emerged as an important avenue for the development of anti-cancer drugs. Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme of the NAD+ salvage pathway, having an SL relationship with nicotinic acid phosphoribosyltransferase (NAPRT), the key enzyme in the NAD+ Preiss-Handler pathway. NAMPT
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Exosomal transfer leads to chemoresistance through oxidative phosphorylation-mediated stemness phenotype in colorectal cancer. Theranostics (IF 12.4) Pub Date : 2023-09-11 Jinhai Deng,Teng Pan,Chunxin Lv,Lulu Cao,Lifeng Li,Xingang Zhou,Gang Li,Huanxin Li,Jose M Vicencio,Yihan Xu,Fengxiang Wei,Yazhou Wang,Zaoqu Liu,Guanglin Zhou,Mingzhu Yin
Background: Recently years have seen the increasing evidence identifying that OXPHOS is involved in different processes of tumor progression and metastasis and has been proposed to be a potential therapeutical target for cancer treatment. However, the exploration in oxidative phosphorylation-mediated chemoresistance is still scarce. In our study, we identify exosomal transfer leads to chemoresistance
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Application of Lung-Targeted Lipid Nanoparticle-delivered mRNA of soluble PD-L1 via SORT Technology in Acute Respiratory Distress Syndrome. Theranostics (IF 12.4) Pub Date : 2023-09-04 Han Sun,Yu Zhang,Jiahui Wang,Juncheng Su,Dejian Zhou,Xiang Yu,Yingjie Xu,Wen Yang
Rationale: Acute respiratory distress syndrome (ARDS) is a life-threatening condition characterized by excessive immune response usually due to lung inflammation. Local immunosuppression is crucial for effective ARDS treatment. However, current methods are limited in their ability to target the lungs specifically. Methods: This study utilized lung-targeted lipid nanoparticles (LNPs) with 1,2-diole
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Detection of lineage-reprogramming efficiency of tumor cells in a 3D-printed liver-on-a-chip model. Theranostics (IF 12.4) Pub Date : 2023-09-04 Zuyan Lu,Xiangwan Miao,Qianqian Song,Huifen Ding,Shiny Amala Priya Rajan,Aleksander Skardal,Konstantinos I Votanopoulos,Kerong Dai,Weixin Zhao,Baisong Lu,Anthony Atala
Background: The liver metastasis accompanied with the loss of liver function is one of the most common complications in patients with triple-negative breast cancers (TNBC). Lineage reprogramming, as a technique direct inducing the functional cell types from one lineage to another lineage without passing through an intermediate pluripotent stage, is promising in changing cell fates and overcoming the
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Blocking Tim-3 enhances the anti-tumor immunity of STING agonist ADU-S100 by unleashing CD4+ T cells through regulating type 2 conventional dendritic cells. Theranostics (IF 12.4) Pub Date : 2023-09-04 Jing Luo,Shuju Pang,Zhenzhen Hui,Hua Zhao,Shilei Xu,Wenwen Yu,Lili Yang,Qian Sun,Xishan Hao,Feng Wei,Jian Wang,Xiubao Ren
Rationale: An immunosuppressive tumor microenvironment (TME) is a major obstacle in tumor immunotherapy. Stimulator of interferon genes (STING) agonists trigger an inflammatory innate immune response to potentially overcome tumor immunosuppression. While STING agonists may hold promise as potential cancer therapy agents, tumor resistance to STING monotherapy has emerged in clinical trials, and the
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Phase I clinical evaluation of 99mTc-labeled Affibody molecule for imaging HER2 expression in breast cancer. Theranostics (IF 12.4) Pub Date : 2023-09-04 Olga Bragina,Vladimir Chernov,Mariia Larkina,Anstasiya Rybina,Roman Zelchan,Eugeniy Garbukov,Maryam Oroujeni,Annika Loftenius,Anna Orlova,Jens Sörensen,Fredrik Y Frejd,Vladimir Tolmachev
The determination of tumor human epidermal growth factor receptor type 2 (HER2) status is of increasing importance with the recent approval of more efficacious HER2-targeted treatments. There is a lack of suitable methods for clinical in vivo HER2 expression assessment. Affibody molecules are small affinity proteins ideal for imaging detection of receptors, which are engineered using a small (molecular
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Targeting redox imbalance in neurodegeneration: characterizing the role of GLP-1 receptor agonists. Theranostics (IF 12.4) Pub Date : 2023-09-04 Puja Ghosh,Rosaria Anna Fontanella,Lucia Scisciola,Ada Pesapane,Fatemeh Taktaz,Martina Franzese,Armando Puocci,Antonio Ceriello,Francesco Prattichizzo,Maria Rosaria Rizzo,Giuseppe Paolisso,Michelangela Barbieri
Reactive oxygen species (ROS) have emerged as essential signaling molecules regulating cell survival, death, inflammation, differentiation, growth, and immune response. Environmental factors, genetic factors, or many pathological condition such as diabetes increase the level of ROS generation by elevating the production of advanced glycation end products, reducing free radical scavengers, increasing
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Oral [60]fullerene reduces neuroinflammation to alleviate Parkinson's disease via regulating gut microbiome. Theranostics (IF 12.4) Pub Date : 2023-09-04 Xue Li,Ruijun Deng,Jie Li,Hui Li,Zhe Xu,Lei Zhang,Linyin Feng,Chunying Shu,Mingming Zhen,Chunru Wang
Neuroinflammation is considered to drive the pathogenic process of neuronal degeneration in Parkinson's disease (PD). However, effective anti-neuroinflammation therapeutics for PD still remain dissatisfactory. Here we explore a robust therapeutic strategy for PD using anti-neuroinflammatory fullerenes. Methods: Oral fullerene was prepared by a ball-milling method. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
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3D autofluorescence imaging of hydronephrosis and renal anatomical structure using cryo-micro-optical sectioning tomography. Theranostics (IF 12.4) Pub Date : 2023-09-04 Guoqing Fan,Chenyu Jiang,Zhuoyao Huang,Mingyu Tian,Huijuan Pan,Yaru Cao,Tian Lei,Qingming Luo,Jing Yuan
Rationale: Mesoscopic visualization of the main anatomical structures of the whole kidney in vivo plays an important role in the pathological diagnosis and exploration of the etiology of hydronephrosis. However, traditional imaging methods cannot achieve whole-kidney imaging with micron resolution under conditions representing in vivo perfusion. Methods: We used in vivo cryofixation (IVCF) to fix acute
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E3 ubiquitin ligase TRIM21 targets TIF1γ to regulate β-catenin signaling in glioblastoma. Theranostics (IF 12.4) Pub Date : 2023-09-04 YanLan Li,Lingbo Bao,Hong Zheng,Mingying Geng,TianYi Chen,Xiaoyan Dai,He Xiao,Lujie Yang,Chengyi Mao,Yuan Qiu,Yu Xu,Dong Wang,Meng Xia Li,Qian Chen
Background: Elucidation of the mechanism of ubiquitation has led to novel ways to treat glioblastoma (GBM). A tripartite motif (TRIM) protein mediates a reversible, stringent ubiquitation which is closely related to glioma malignancy. This study intends to screen the most vital and abnormal regulating component of the tripartite motif protein and to explore its underlying mechanisms. Methods: TRIM21
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Polymer theranostics with multiple stimuli-based activation of photodynamic therapy and tumor imaging. Theranostics (IF 12.4) Pub Date : 2023-09-04 Marina Rodrigues Tavares,Rayhanul Islam,Vladimír Šubr,Steffen Hackbarth,Shanghui Gao,Kai Yang,Volodymyr Lobaz,Jun Fang,Tomáš Etrych
Background: Efficient theranostic strategies concurrently bring and use both the therapeutic and diagnostic features, serving as a cutting-edge tool to combat advanced cancers. Goals of the Investigation: Here, we develop stimuli-sensitive theranostics consisting of tailored copolymers forming micellar conjugates carrying pyropheophorbide-a (PyF) attached by pH-sensitive hydrazone bonds, thus enabling
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Targeting cullin neddylation for cancer and fibrotic diseases. Theranostics (IF 12.4) Pub Date : 2023-09-04 Zhang-Xu He,Wei-Guang Yang,Dan Zengyangzong,Ge Gao,Qian Zhang,Hong-Min Liu,Wen Zhao,Li-Ying Ma
Protein neddylation is a post-translational modification, and its best recognized substrates are cullin family proteins, which are the core component of Cullin-RING ligases (CRLs). Given that most neddylation pathway proteins are overactivated in different cancers and fibrotic diseases, targeting neddylation becomes an emerging approach for the treatment of these diseases. To date, numerous neddylation
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Metabolite Neu5Ac triggers SLC3A2 degradation promoting vascular endothelial ferroptosis and aggravates atherosclerosis progression in ApoE-/-mice. Theranostics (IF 12.4) Pub Date : 2023-09-04 Peng Xiang,Qingqiu Chen,Le Chen,Jin Lei,Zhiyi Yuan,Hui Hu,Yining Lu,Xianmin Wang,Tingting Wang,Ruihong Yu,Wanping Zhang,Jun Zhang,Chao Yu,Limei Ma
Background: Atherosclerosis (AS) is still the major cause of cardiovascular disease (CVD) as well as stroke. Endothelial metabolic disorder has been found to be activated and then promote endothelial cells (ECs) injury, which is regarded to initiate AS progression. N-acetylneuraminic acid (Neu5Ac), a metabolite produced by hexosamine-sialic acid pathway branching from glucose metabolism, was presented