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Using encrusting bryozoan adhesion to evaluate the efficacy of fouling-release marine coatings Biofouling (IF 2.351) Pub Date : 2020-12-20 G. T. Waltz; K. Z. Hunsucker; G. Swain; D. E. Wendt
Abstract Biofouling communities are spatiotemporally diverse, underscoring the need to assess fouling-release (FR) coating performance against common biofouling taxa at multiple field sites. Adhesion strength assessments of FR coatings incorporate few taxa into standardized protocols. This study tested the feasibility of incorporating existing ASTM barnacle protocols on tubeworms and encrusting bryozoans
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Promising treatment strategies to combat Staphylococcus aureus biofilm infections: an updated review Biofouling (IF 2.351) Pub Date : 2020-12-22 P. S. Seethalakshmi; Riya Rajeev; George Seghal Kiran; Joseph Selvin
Abstract Staphylococcus aureus is a leading cause of nosocomial and community-acquired infections. The formation of biofilm by this pathogen renders it resilient to antimicrobial agents, which complicates the treatment of such infections. S. aureus can form biofilms with other pathogens and cause polymicrobial infections recalcitrant to antimicrobial agents. Therefore, anti-biofilm agents against which
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Effect of xylitol tablets with and without red propolis on salivary parameters, dental biofilm and sensory acceptability of adolescents: a randomized crossover clinical trial Biofouling (IF 2.351) Pub Date : 2020-12-17 Mariana Leonel Martins; Amanda Souza Nunes Monteiro; Thiago Isidro Vieira; Maria Bárbara de Carvalho Torres Guimarães; Letícia Coli Louvisse de Abreu; Lucio Mendes Cabral; Yuri Wanderley Cavalcanti; Lucianne Cople Maia; Andréa Fonseca-Gonçalves
Abstract The effect of xylitol tablets with (XPT) and without (XT) red propolis on salivary parameters, dental biofilm and acceptability of adolescents was evaluated through a blinded randomized crossover clinical trial. Healthy volunteers were allocated in the XPT and XT groups with a 30-day washout period for consumption of two tablets/dayfor seven days. An increase in salivary parameters was only
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Establishment of microcosm biofilm models that reproduce a cariogenic diet intake Biofouling (IF 2.351) Pub Date : 2020-12-21 Elkin Jahir Florez Salamanca; Rayssa Mariana Dantas; Martha Juliana Rodriguez; Marlise Inêz Klein
(2020). Establishment of microcosm biofilm models that reproduce a cariogenic diet intake. Biofouling: Vol. 36, No. 10, pp. 1196-1209.
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Marine natural products as antifouling molecules – a mini-review (2014–2020) Biofouling (IF 2.351) Pub Date : 2021-01-05 Ling-Li Liu; Chuan-Hai Wu; Pei-Yuan Qian
Abstract In the present review, 182 antifouling (AF) natural products from marine microorganisms, algae and marine invertebrates reported from August 2014 to May 2020 are presented. Amongst these compounds, over half were isolated from marine-derived microorganisms, including 70 compounds from fungi and 31 compounds from bacteria. The structure–relationship of some of these compounds is also briefly
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A statistical approach for modelling the physical process of bacterial attachment to abiotic surfaces Biofouling (IF 2.351) Pub Date : 2021-01-07 Yi Wang; Sui M. Lee; Ian R. Gentle; Gary A. Dykes
Abstract A statistical approach using a polynomial linear model in combination with a probability distribution model was developed to mathematically represent the process of bacterial attachment and study its mechanism. The linear deterministic model was built based on data from experiments investigating bacterial and substratum surface physico-chemical factors as predictors of attachment. The prediction
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The effect of physico-chemical treatment in reducing Listeria monocytogenes biofilms on lettuce leaf surfaces Biofouling (IF 2.351) Pub Date : 2021-01-05 Md. Furkanur Rahaman Mizan; Hye Ran Cho; Md. Ashrafudoulla; Junbin Cho; Md. Iqbal Hossain; Dong-Un Lee; Sang-Do Ha
(2020). The effect of physico-chemical treatment in reducing Listeria monocytogenes biofilms on lettuce leaf surfaces. Biofouling: Vol. 36, No. 10, pp. 1243-1255.
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Synergistic antimicrobial combination of carvacrol and thymol impairs single and mixed-species biofilms of Candida albicans and Staphylococcus epidermidis Biofouling (IF 2.351) Pub Date : 2021-01-12 Thirukannamangai Krishnan Swetha; Arumugam Vikraman; Chari Nithya; Nagaiah Hari Prasath; Shunmugiah Karutha Pandian
Abstract Candida albicans and Staphylococcus epidermidis are important opportunistic human pathogens, which form mixed-species biofilms and cause recalcitrant device associated infections in clinical settings. Further to many reports suggesting the therapeutic potential of plant-derived monoterpenoids, this study investigated the interaction of the monoterpenoids carvacrol (C) and thymol (T) against
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Influence of biofilm removal from the tooth-restoration interface on the progression of secondary caries lesions: a preliminary in vitro model study Biofouling (IF 2.351) Pub Date : 2021-01-05 Cácia Signori; Tamires Timm Maske; Vitor Henrique Digmayer Romero; Maximiliano Sérgio Cenci
Abstract This study investigated the effects of biofilm removal from the tooth-restoration surface on secondary caries lesion progression. Biofilms were grown for up to 28 days on resin-restored enamel-dentin disks with an interfacial gap with DMM or DMM + 1% sucrose under five different protocols of Visible Biofilm Removal (V-BR; n = 7): (1) without V-BR, (2) with partial V-BR, (3) with total V-BR
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Synergistic antimicrobial combination of carvacrol and thymol impairs single and mixed-species biofilms of Candida albicans and Staphylococcus epidermidis Biofouling (IF 2.351) Pub Date : 2021-01-12 Thirukannamangai Krishnan Swetha; Arumugam Vikraman; Chari Nithya; Nagaiah Hari Prasath; Shunmugiah Karutha Pandian
Abstract Candida albicans and Staphylococcus epidermidis are important opportunistic human pathogens, which form mixed-species biofilms and cause recalcitrant device associated infections in clinical settings. Further to many reports suggesting the therapeutic potential of plant-derived monoterpenoids, this study investigated the interaction of the monoterpenoids carvacrol (C) and thymol (T) against
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Interaction of the surface bound antimicrobial peptides melimine and Mel4 with Staphylococcus aureus Biofouling (IF 2.351) Pub Date : 2020-11-08 Muhammad Yasir; Debarun Dutta; Naresh Kumar; Mark D. P. Willcox
Abstract Melimine and Mel4 are cationic antimicrobial peptides which can resist biofilm development once bound to biomaterials. The aim of the current study was to determine the mode of action of bound melimine and Mel4 against S. aureus. The peptides were covalently attached to glass using an azidobenzoic acid linker. The amount of attached peptides was confirmed by XPS and amino acid analysis and
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Phenolic extract of Eugenia uniflora L. and furanone reduce biofilm formation by Serratia liquefaciens and increase its susceptibility to antimicrobials Biofouling (IF 2.351) Pub Date : 2020-11-13 Adeline Conceição Rodrigues; Felipe Alves de Almeida; Cleriane André; Maria Cristina Dantas Vanetti; Uelinton Manoel Pinto; Neuza Mariko Aymoto Hassimotto; Érica Nascif Rufino Vieira; Nélio José de Andrade
Abstract Serratia liquefaciens is a spoilage microorganism of relevance in the dairy industry because it is psychrotrophic, able to form biofilm, and produces thermoresistant proteases and lipases. Phenolic compounds and furanones have been studied as inhibitors of biofilm formation. In this study, the potential of the pulp phenolic extract of Eugenia uniflora L. orange fruits, also called pitanga
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Degradable hyaluronic acid/chitosan polyelectrolyte multilayers with marine fouling-release properties Biofouling (IF 2.351) Pub Date : 2020-11-29 Wenfa Yu; Robin Wanka; John A. Finlay; Jessica L. Clarke; Anthony S. Clare; Axel Rosenhahn
Abstract Polysaccharide multilayers consisting of hyaluronic acid and chitosan were prepared by layer-by-layer assembly. To be used in seawater, the multilayers were crosslinked to a different degree using thermal or chemical methods. ATR-FTIR revealed different amide densities as a result of the crosslinking conditions. AFM showed that the crosslinking affected the roughness and swelling behavior
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Identification of bacterial biofilms on desalination reverse osmosis membranes from the mediterranean sea Biofouling (IF 2.351) Pub Date : 2020-11-24 Zakaria Benladghem; Sidi Mohamed Lahbib Seddiki; Yassine Moustafa Mahdad
Abstract Nanofiltration and reverse osmosis are two of the most effective surface water treatment processes. They provide water of high quality and eliminate a large amount of microorganisms, organic matter and micropollutants. However, the main limitation of membrane nanofiltration is fouling, which imposes an additional cost. This study focused on the search for microorganisms capable of reducing
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Effects of ‘in-service’ conditions – mimicked hull roughness ranges and biofilms – on the surface and the hydrodynamic characteristics of foul-release type coatings Biofouling (IF 2.351) Pub Date : 2020-12-08 I. A. Yeginbayeva; M. Atlar; S. Turkmen; H. Chen
Abstract To develop a better understanding of ‘in-service’ performance of modern marine coatings, this study explored the combined effects of different roughness ranges of foul-release coating (FRC) and light biofouling (slime) on the surface, boundary layer and drag characteristics under a range of ‘in-service’ conditions. Natural and laboratory biofilms were grown dynamically on FRC panels by exposing
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Effect of water aging on the anti-biofilm properties of glass ionomer cement containing fluoro-zinc-silicate fillers Biofouling (IF 2.351) Pub Date : 2020-12-21 Traithawit Naksagoon; Tatsuya Ohsumi; Shoji Takenaka; Ryoko Nagata; Taisuke Hasegawa; Takeyasu Maeda; Yuichiro Noiri
Abstract A previous study has reported that a novel fluoro-zinc-silicate glass ionomer cement (Caredyne Restore) showed superior anti-biofilm effects by interfering with bacterial adhesion. However, the active ions may degrade with time. This study aimed to assess the valid anti-biofilm effects of Caredyne Restore after being aged by water immersion for 3 weeks. Streptococcus mutans biofilm was allowed
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Genes and SNPs in the pathway of immune response and caries risk: a systematic review and meta-analysis Biofouling (IF 2.351) Pub Date : 2020-12-17 Luiz Alexandre Chisini; Mariana Gonzalez Cademartori; Marucs Cristian Muniz Conde; Francine Dos Santos Costa; Luciana Tovo-Rodrigues; Rodrigo Varella de Carvalho; Flávio Fernando Demarco; Marcos Britto Correa
Abstract The aim of this systematic review and meta-analysis was to pool the data on Single Nucleotide Polymorphisms (SNPs) in immune response genes associated with dental caries. Nineteen studies were included in the review and 18 in the meta-analysis. Twenty-two SNPs were evaluated, which are linked to six different genes (MBL2, LFT, MASP2, DEFB1, FCN2 and MUC5B). Most SNPs (81.8%) are related to
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DNase-mediated eDNA removal enhances D-LL-31 activity against biofilms of bacteria isolated from chronic rhinosinusitis patients Biofouling (IF 2.351) Pub Date : 2020-12-09 Saharut Wongkaewkhiaw; Sakawrat Kanthawong; Jan G. M. Bolscher; Kamran Nazmi; Suwimol Taweechaisupapong; Bastiaan P. Krom
Abstract Chronic rhinosinusitis (CRS) is a chronic infection of the nasal cavity and paranasal sinuses associated with the presence of a microbial biofilm. Extracellular DNA (eDNA) is an important component of the biofilm matrix. Antimicrobial peptides (AMPs) are natural peptides with the ability to kill microorganisms. D-LL-31 is a synthetic variant of the AMP cathelicidin with increased resistance
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Mini-review: from in vitro to ex vivo studies: an overview of alternative methods for the study of medical biofilms Biofouling (IF 2.351) Pub Date : 2020-12-21 Débora de Souza Collares Maia Castelo-Branco; Bruno Rocha Amando; Crister José Ocadaque; Lara de Aguiar; Débora Damásio de Queiroz Paiva; Expedito Maia Diógenes; Glaucia Morgana de Melo Guedes; Cecília Leite Costa; Anísio Silvestre Pinheiro Santos-Filho; Ana Raquel Colares de Andrade; Rossana de Aguiar Cordeiro; Marcos Fábio Gadelha Rocha; José Júlio Costa Sidrim
Abstract Microbial biofilms are a natural adaptation of microorganisms, typically composed of multiple microbial species, exhibiting complex community organization and cooperation. Biofilm dynamics and their complex architecture are challenging for basic analyses, including the number of viable cells, biomass accumulation, biofilm morphology, among others. The methods used to study biofilms range from
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Genes and SNPs in the pathway of immune response and caries risk: a systematic review and meta-analysis Biofouling (IF 2.351) Pub Date : 2020-12-17 Luiz Alexandre Chisini; Mariana Gonzalez Cademartori; Marucs Cristian Muniz Conde; Francine Dos Santos Costa; Luciana Tovo-Rodrigues; Rodrigo Varella de Carvalho; Flávio Fernando Demarco; Marcos Britto Correa
Abstract The aim of this systematic review and meta-analysis was to pool the data on Single Nucleotide Polymorphisms (SNPs) in immune response genes associated with dental caries. Nineteen studies were included in the review and 18 in the meta-analysis. Twenty-two SNPs were evaluated, which are linked to six different genes (MBL2, LFT, MASP2, DEFB1, FCN2 and MUC5B). Most SNPs (81.8%) are related to
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Effects of ‘in-service’ conditions – mimicked hull roughness ranges and biofilms – on the surface and the hydrodynamic characteristics of foul-release type coatings Biofouling (IF 2.351) Pub Date : 2020-12-08 I. A. Yeginbayeva; M. Atlar; S. Turkmen; H. Chen
Abstract To develop a better understanding of ‘in-service’ performance of modern marine coatings, this study explored the combined effects of different roughness ranges of foul-release coating (FRC) and light biofouling (slime) on the surface, boundary layer and drag characteristics under a range of ‘in-service’ conditions. Natural and laboratory biofilms were grown dynamically on FRC panels by exposing
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Molecules involved in motility regulation in Escherichia coli cells: a review Biofouling (IF 2.351) Pub Date : 2020-10-07 Fazlurrahman Khan; Nazia Tabassum; Dung Thuy Nguyen Pham; Sandra Folarin Oloketuyi; Young-Mog Kim
Abstract The initial colonization of the host organism by commensal, probiotic, and pathogenic Escherichia coli strains is an important step in the development of infections and biofilms. Sensing and colonization of host cell surfaces are governed by flagellar and fimbriae/pili appendages, respectively. Biofilm formation confers great advantages on pathogenic E. coli cells such as protection against
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Exogenous fungal quorum sensing molecules inhibit planktonic cell growth and modulate filamentation and biofilm formation in the Sporothrix schenckii complex Biofouling (IF 2.351) Pub Date : 2020-10-15 Raimunda Sâmia Nogueira Brilhante; Vandbergue Santos Pereira; Augusto Feynman Dias Nobre; Jonathas Sales de Oliveira; Mirele Rodrigues Fernandes; Anderson da Cunha Costa; Anderson Messias Rodrigues; Zoilo Pires de Camargo; Waldemiro Aquino Pereira-Neto; José Júlio Costa Sidrim; Marcos Fábio Gadelha Rocha
Abstract This study investigated the effect of the quorum sensing molecules (QSMs) farnesol, 2-phenylehtanol, tyrosol and tryptophol against planktonic cells, filamentation and biofilms of Sporothrix spp. The antifungal activity of QSMs was evaluated by broth microdilution. QSMs showed MICs in the ranges of 0.01-1 µM (farnesol), 1-8 mM (2-phenylehtanol and tyrosol), and >16 mM (tryptophol). Filamentous
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Quorum quenching activity of pentacyclic triterpenoids leads to inhibition of biofilm formation by Acinetobacter baumannii Biofouling (IF 2.351) Pub Date : 2020-10-25 Sudipta Paul Bhattacharya; Akash Mitra; Arijit Bhattacharya; Aparna Sen
Abstract The quorum quenching (QQ) potential of three pentacyclic triterpenoids, glycyrrhetinic acid (GRA), ursolic acid (UA) and betulinic acid (BA), representing distinct groups of compounds, was evaluated. Violacein production by Chromobacterium violaceum and pyocyanin production by Pseudomonas aeruginosa were severely affected by GRA, UA and BA, suggesting a perturbation of N-acyl homoserine lactone
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Variability in in vitro biofilm production and antimicrobial sensitivity pattern among Pasteurella multocida strains Biofouling (IF 2.351) Pub Date : 2020-10-15 Awadhesh Prajapati; Mohammed Mudassar Chanda; Arul Dhayalan; Revanaiah Yogisharadhya; Jitendra Kumar Chaudhary; Nihar Nalini Mohanty; Sathish Bhadravati Shivachandra
Abstract Biofilm production, hitherto an uncharacterized feature among circulating Pasteurella multocida strains, was studied along with the antibiotic susceptibility pattern. On the basis of biofilm formation ability, all the strains were categorized into four groups under six different culture conditions: strong biofilm-forming (22%), moderate (19%), weak (51%), and non-adherent (7%). Strains from
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Underwater shear-based grooming of marine biofouling using a non-contact Bernoulli pad device Biofouling (IF 2.351) Pub Date : 2020-10-25 Kristina M. Kamensky; Aren M. Hellum; Ranjan Mukherjee; Abhishek Naik; Pia H. Moisander
Abstract The biofouling removal ability of a shear-based device was tested on two submerged surface types, Garolite G-10 and Intersleek 1100SR. Each surface was groomed at four frequencies along with a control group. The seven-week grooming study was conducted in Narragansett Bay, Rhode Island. The Bernoulli pad device uses confined radial outflow to generate both surface-normal forces to grip the
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Effect of quorum sensing and quenching molecules on inter-kingdom biofilm formation by Penicillium expansum and bacteria Biofouling (IF 2.351) Pub Date : 2020-10-20 Tiago Barros Afonso; Lúcia Chaves Simões; Nelson Lima
Abstract The ecology of a biofilm is a complex function of different factors, including the presence of microbial metabolites excreted by the inhabitants of the biofilm. This study aimed to assess the effect of patulin, and N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL) on inter-kingdom biofilm formation between a filamentous fungus and bacteria isolated from drinking water. The filamentous
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Characterization of the biofilm grown on 304L stainless steel in urban wastewaters: extracellular polymeric substances (EPS) and bacterial consortia Biofouling (IF 2.351) Pub Date : 2020-10-22 Islem Ziadi; Leila El-Bassi; Latifa Bousselmi; Hanene Akrout
Abstract Characterization of the biofilm growing on stainless steel (SS) in untreated (UTUWW) and treated (TUWW) urban wastewaters was performed. In both media, the first phase of biofilm growth was aerobic, when the genera Caldimonas, Caulobacter, Terriglobus and Edaphobacter (iron oxidizing bacteria [IOB]) and the genera Bacillus, Sulfurimonas, Syntrophobacter and Desulfobacter (sulfur oxidizing
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Rhizobacterial biofilm and plant growth promoting trait enhancement by organic acids and sugars Biofouling (IF 2.351) Pub Date : 2020-11-04 Jishma Panichikkal; Radhakrishnan Edayileveetil Krishnankutty
Abstract In the study,Pseudomonas sp. K6 and Pseudomonas monteilii were found to form an enhanced biofilm when cultured in the presence of organic acids and sugars. Here, the highest biofilm could be observed for Pseudomonas sp. K6 (3.08 ± 0.13) and P. monteilii (1.99 ± 0.12) when cultured in presence of 10 µM malic acid. However, maximum production of indole 3 acetic acid (IAA) was observed with 25 µM
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Benzyl isocyanate isolated from the leaves of Psidium guajava inhibits Staphylococcus aureus biofilm formation Biofouling (IF 2.351) Pub Date : 2020-11-10 Kunal Dutta; Amit Karmakar; Debarati Jana; Saroj Ballav; Sergey Shityakov; Amiya Kumar Panda; Chandradipa Ghosh
Abstract Benzyl isocyanate (BIC), from methanol extract of Psidium guajava leaves, exhibited substantial anti-biofilm activities against Staphylococcus aureus, the common bacterial pathogen in nosocomial infections. Major components of the extract included eugenol, BIC, phenyl-2-methoxy-4-(1-propenyl)-acetate and 2,5-pyrrolidinedione,1-penta-3-4-dienyl, analyzed by GC-MS and HPLC studies. BIC exhibited
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A bacterial polysaccharide biosynthesis-related gene inversely regulates larval settlement and metamorphosis of Mytilus coruscus. Biofouling (IF 2.351) Pub Date : 2020-08-26 Li-Hua Peng,Xiao Liang,Rui-Heng Chang,Jia-Yi Mu,Hui-E Chen,Asami Yoshida,Kiyoshi Osatomi,Jin-Long Yang
Abstract Larval settlement and metamorphosis is essential for the development of marine invertebrates. Although polysaccharides are involved in larval settlement and metamorphosis of Mytilus coruscus, the molecular basis of polysaccharides underlying this progression remains largely unknown. Here, the roles of the polysaccharide biosynthesis-related gene 01912 of Pseudoalteromonas marina ECSMB14103
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Endowing antifouling properties on metal substrata by creating an artificial barrier layer based on scalable metal oxide nanostructures. Biofouling (IF 2.351) Pub Date : 2020-08-25 Kyounghwan Song,Jaehwan Shim,Jung-Yeul Jung,Choongyeop Lee,Youngsuk Nam
Abstract Here, by creating different types of artificial barrier layer against bacterial attachment, anti-biofouling properties were endowed on three metallic surfaces – aluminum, stainless steel and titanium. To each metallic surface, a tailored chemical oxidation process was applied to grow scalable oxide structures with an additional appropriate coating, resulting in three different types of anti-biofouling
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In vitro and ex vivo biofilms of dermatophytes: a new panorama for the study of antifungal drugs. Biofouling (IF 2.351) Pub Date : 2020-08-25 Débora de Souza Collares Maia Castelo-Branco,Lara de Aguiar,Géssica Dos Santos Araújo,Raissa Geovanna Pereira Lopes,Jamile de Alencar Sales,Waldemiro Aquino Pereira-Neto,Adriana de Queiroz Pinheiro,Germana Costa Paixão,Rossana de Aguiar Cordeiro,José Júlio Costa Sidrim,Raimunda Sâmia Nogueira Brilhante,Marcos Fábio Gadelha Rocha
Abstract This study describes an ex vivo model that creates an environment for dermatophyte biofilm growth, with features that resemble those of in vivo conditions, designing a new panorama for the study of antifungal susceptibility. Regarding planktonic susceptibility, MIC ranges were 0.125-1 µg ml−1 for griseofulvin and 0.000097-0.25 µg ml−1 for itraconazole and terbinafine. sMIC50 ranges were 2->512 µg
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Mechano-chemical coupling of irrigation enhances endodontic biofilm debridement. Biofouling (IF 2.351) Pub Date : 2020-09-01 Jialei Xu,Yuan Gao,Yajun Meng,Weiwei Wu,Chialing Tsauo,Tingwei Guo,Yangpei Cao,Dingming Huang,Xuedong Zhou,Jinzhi He
Abstract The complexity of the root canal system results in areas where mechanical instrumentation is impossible during endodontic treatment. To disinfect these areas, the effect of irrigation on biofilm debridement is of great significance but has not yet been well explored. Using an in vitro Enterococcus faecalis biofilm model and a biofilm reactor, the present study provides a better understanding
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Effect of phosphate availability on biofilm formation in cooling towers. Biofouling (IF 2.351) Pub Date : 2020-09-04 Ingrid S M Pinel,Lan Hee Kim,Vitor R Proença Borges,Nadia M Farhat,Geert-Jan Witkamp,Mark C M van Loosdrecht,Johannes S Vrouwenvelder
Abstract Phosphate limitation has been suggested as a preventive method against biofilms. P-limited feed water was studied as a preventive strategy against biofouling in cooling towers (CTs). Three pilot-scale open recirculating CTs were operated in parallel for five weeks. RO permeate was fed to the CTs (1) without supplementation (reference), (2) with supplementation by biodegradable carbon (P-limited)
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Surface modification of polystyrene Petri dishes by plasma polymerized 4,7,10-trioxa-1,13-tridecanediamine for enhanced culturing and migration of bovine aortic endothelial cells. Biofouling (IF 2.351) Pub Date : 2020-09-17 Hyuna Lim,Yoonsoo Park,Youngsik Seo,Heonyong Park,Yong Ki Cho,Donggeun Jung
Abstract Plasma surface modification is an effective method for changing material properties to control cell behavior on a surface. This study investigates the efficiency of a plasma polymerized 4,7,10-trioxa-1,13-tridecanediamine (ppTTDDA) film coated on a polystyrene (PS) Petri dish, which is a biocompatible surface with carbon- and oxygen-based chemical species. The adhesion, proliferation, and
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Antimicrobial activity of Curcuma xanthorrhiza nanoemulsions on Streptococcus mutans biofilms Biofouling (IF 2.351) Pub Date : 2020-09-24 Mu-Yeol Cho; Si-Mook Kang; Eun-Song Lee; Baek-Il Kim
Abstract In this study, an optimal nanoemulsion formulation for Curcuma xanthorrhiza oil (Xan) was investigated using different sonication times. The antimicrobial effects of the nanoemulsion, the original emulsion, distilled water (DW), and Listerine, on Streptococcus mutans biofilms were compared. The optimum ultrasonic time, determined in terms of droplet size and stability, was found to be 10 min
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Effects of Brazilian green propolis extract on planktonic cells and biofilms of multidrug-resistant strains of Klebsiella pneumoniae and Pseudomonas aeruginosa. Biofouling (IF 2.351) Pub Date : 2020-09-21 Pâmela Beatriz do Rosário Estevam Dos Santos,Damara da Silva Ávila,Lucas de Paula Ramos,Amanda Romagnoli Yu,Carlos Eduardo da Rocha Santos,Andresa Aparecida Berretta,Samira Esteves Afonso Camargo,Jonatas Rafael de Oliveira,Luciane Dias de Oliveira
Abstract Propolis could represent an alternative therapeutic agent for targeting multidrug-resistant bacteria due to its antimicrobial potential. The effect of Brazilian green propolis (BGP) aqueous extract (AqExt) was evaluated on eight multidrug-resistant clinical strains of Klebsiella pneumoniae and Pseudomonas aeruginosa, as well as on one reference strain for each bacterial species. The minimum
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Enterococcus faecalis inhibits Klebsiella pneumoniae growth in polymicrobial biofilms in a glucose-enriched medium Biofouling (IF 2.351) Pub Date : 2020-09-24 Victoria Ballén; Carlos Ratia; Virginio Cepas; Sara M. Soto
Abstract Catheter-related urinary tract infections are one of the most common biofilm-associated diseases. Within biofilms, bacteria cooperate, compete, or have neutral interactions. This study aimed to investigate the interactions in polymicrobial biofilms of Klebsiella pneumoniae and Enterococcus faecalis, two of the most common uropathogens. Although K. pneumoniae was the most adherent strain, it
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Antimicrobial effects of a pulsed electromagnetic field: an in vitro polymicrobial periodontal subgingival biofilm model Biofouling (IF 2.351) Pub Date : 2020-09-29 Marcelo Faveri; Danilo Eduardo Calgaro Miquelleto; Bruno Bueno-Silva; João Marcos Spessoto Pingueiro; Luciene Cristina Figueiredo; Oleg Dolkart; Elad Yakobson; Shlomo Barak; Magda Feres; Jamil Awad Shibli
Abstract The objective was to test the influence of a pulsed electromagnetic field (PEMF) on bacterial biofilm colonization around implants incorporated with healing abutments. Healing abutments with (test group) and without (control group) active PEMF devices were placed in a multispecies biofilm consisting of 31 different bacterial species. The biofilm composition and total bacterial counts (x105)
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On the release of fluoride from biofilm reservoirs during a cariogenic challenge: an in situ study Biofouling (IF 2.351) Pub Date : 2020-09-29 João G. S. Souza; Altair A. Del Bel Cury; Jaime A. Cury; Livia M. A. Tenuta
Abstract Biofilm fluoride reservoirs may be a source of fluoride to the fluid phase during a sugar challenge reducing tooth mineral loss. However, the evidence for that is conflicting and has not been studied in biofilms containing different fluoride levels. In order to test fluoride release from biofilms with distinct fluoride concentrations, biofilms were grown in situ exposed to a combination of
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Characterization of polymicrobial biofilms obtained from saliva or carious lesions in dentin Biofouling (IF 2.351) Pub Date : 2020-10-01 Aline Leite de Farias; Lorena Petrucelli Freire de Carvalho; Daniela Alejandra Cusicanqui Méndez; Thiago Cruvinel; Fernanda Lourenção Brighenti
Abstract This study aimed to compare the formation of polymicrobial biofilms using carious dentin or saliva as inoculum for application in in vitro microbiological studies on caries research. For biofilm growth, combined samples of infected dentin or saliva from three donors were used. The biofilms were grown on glass coverslips, under a regimen of intermittent exposure (6 h day−1) to 1% sucrose for
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Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach. Biofouling (IF 2.351) Pub Date : 2020-07-26 Maria João Leal Romeu,Dany Domínguez-Pérez,Daniela Almeida,João Morais,Alexandre Campos,Vítor Vasconcelos,Filipe J M Mergulhão
Cyanobacteria promote marine biofouling with significant impacts. A qualitative proteomic analysis, by LC-MS/MS, of planktonic and biofilm cells from two cyanobacteria was performed. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s−1 and 40 s−1). For both strains and surfaces, biofilm development was higher at 4 s−1
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Effects of crosslink density in zwitterionic hydrogel coatings on their antifouling performance and susceptibility to silt uptake. Biofouling (IF 2.351) Pub Date : 2020-07-27 Julian Koc,Eric Schönemann,Robin Wanka,Nick Aldred,Anthony S Clare,Harrison Gardner,Geoffrey W Swain,Kelli Hunsucker,Andre Laschewsky,Axel Rosenhahn
Hydrogel coatings effectively reduce the attachment of proteins and organisms in laboratory assays, in particular when made from zwitterionic monomers. In field experiments with multiple species and non-living material, such coatings suffer from adsorption of particulate matter. In this study, the zwitterionic monomer 3-[N-(2-methacryloyloxyethyl)-N,N-dimethylammonio] propanesulfonate (SPE) was copolymerized
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Application of zinc oxide and sodium alginate for biofouling mitigation in a membrane bioreactor treating urban wastewater. Biofouling (IF 2.351) Pub Date : 2020-08-04 Fatemeh Sokhandan,Maryam Homayoonfal,Fatemeh Davar
(2020). Application of zinc oxide and sodium alginate for biofouling mitigation in a membrane bioreactor treating urban wastewater. Biofouling: Vol. 36, No. 6, pp. 660-678.
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Destruction of Pseudomonas aeruginosa pre-formed biofilms by cationic polymer micelles bearing silver nanoparticles. Biofouling (IF 2.351) Pub Date : 2020-08-03 Tsvetelina Paunova-Krasteva,Emi Haladjova,Petar Petrov,Aleksander Forys,Barbara Trzebicka,Tanya Topouzova-Hristova,Stoyanka R Stoitsova
Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen often associated with biofilm infections. This study evaluated the capacity for biofilm destruction of a novel combination of cationic polymer micelles formed from poly(2-(dimethylamino)ethyl methacrylate)-b-poly(ε-caprolactone)-b-poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA-PCL-PDMAEMA) triblock copolymer either alone, or loaded
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Effect of arginine on microorganisms involved in dental caries: a systematic literature review of in vitro studies. Biofouling (IF 2.351) Pub Date : 2020-08-04 Marina Lins Miranda,Bianca Núbia Souza Silva,Karina Borges Salomão,Analú Barros de Oliveira,Paulo Roberto Gabbai-Armelin,Fernanda Lourenção Brighenti
This systematic review aimed to discuss the effects of arginine on caries-related microorganisms in different in vitro biofilm models. The eligibility criteria were in vitro studies that evaluated the effect of arginine at different concentrations on caries-related microorganisms using biofilm models. Eighteen studies published between 2012 and 2019 were included. Different bacterial species were studied
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Synergistic antibacterial and anti-biofilm activity of nisin like bacteriocin with curcumin and cinnamaldehyde against ESBL and MBL producing clinical strains. Biofouling (IF 2.351) Pub Date : 2020-08-09 Garima Sharma,Shweta Dang,Aruna K,Manjula Kalia,Reema Gabrani
Bacteriocins are small peptides that can inhibit the growth of a diverse range of microbes. There is a need to identify bacteriocins that are effective against biofilms of resistant clinical strains. The present study focussed on the efficacy of purified nisin like bacteriocin-GAM217 against extended spectrum β-lactamase (ESBL) and metallo-beta-lactamase (MBL) producing clinical strains. Bacteriocin-GAM217
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Development of a multispecies periodontal biofilm model within a stirred bioreactor. Biofouling (IF 2.351) Pub Date : 2020-08-11 Maick Meneguzzo Prado,Davi J Kovalski,Willy Bustillos Torrez,Bruno Bueno-Silva,Magda Feres,Josiane de Almeida,Luismar M Porto
The objective of this work was to develop a subgingival biofilm model using a stirred bioreactor. Discs of bovine teeth were adapted to a stirred bioreactor filled with a culture medium containing bacterial species associated with periodontal health or disease. After anaerobic incubation, the biofilms growing on the substratum surfaces were collected and analyzed. The mean number of Colony-forming
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Drag production mechanisms of filamentous biofilms. Biofouling (IF 2.351) Pub Date : 2020-08-18 Joel D Hartenberger,Elizabeth G Callison,James W Gose,Marc Perlin,Steven L Ceccio
Biofilms were grown on smooth acrylic surfaces for nominal incubation times of three, five, and ten weeks in a flow loop at the University of Michigan. The biofilm covered surfaces were exposed to the turbulent flow in a high-aspect ratio, fully developed channel flow facility at height-based Reynolds numbers from ReH ≈ 5,000 to 30,000. Measurements of the pressure drop along each fouled upper surface
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Characterization and removal of biofouling from reverse osmosis membranes (ROMs) from a desalination plant in Northern Chile, using Alteromonas sp. Ni1-LEM supernatant. Biofouling (IF 2.351) Pub Date : 2020-06-16 Hernán Vera-Villalobos,Vilma Pérez,Francisco Contreras,Valezka Alcayaga,Vladimir Avalos,Carlos Riquelme,Fernando Silva-Aciares
Biofouling control in reverse osmosis membranes (ROMs) is challenging due to the high cost of treatments, and reduction in the life of ROMs. This study characterizes the biofouling in the ROMs from a desalination plant and reports its effective removal using the supernatant obtained from Alteromonas sp. strain Ni1-LEM. The characterization of the bacterial community revealed that the most abundant
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Development of a novel anti-biofilm peptide derived from profilin of Spodoptera frugiperda. Biofouling (IF 2.351) Pub Date : 2020-07-03 Amanda Carolina Borges da Silva,Janaina de Cassia Orlandi Sardi,Daniella Gorete Lourenço de Oliveira,Caio Fernando Ramalho de Oliveira,Helder Freitas Dos Santos,Edson Lucas Dos Santos,Edson Crusca,Marlon Henrique Cardoso,Octávio Luiz Franco,Maria Lígia Rodrigues Macedo
Candida yeast infections are the fourth leading cause of death worldwide. Peptides with antimicrobial activity are a promising alternative treatment for such infections. Here, the antifungal activity of a new antimicrobial peptide—PEP-IA18—was evaluated against Candida species. PEP-IA18 was designed from the primary sequence of profilin, a protein from Spodoptera frugiperda, and displayed potent activity
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Diclofenac exhibits synergism with azoles against planktonic cells and biofilms of Candida tropicalis. Biofouling (IF 2.351) Pub Date : 2020-06-16 Raimunda Sâmia Nogueira Brilhante,Jaiane Alves Brasil,Jonathas Sales de Oliveira,Vandbergue Santos Pereira,Waldemiro de Aquino Pereira-Neto,José Júlio Costa Sidrim,Marcos Fábio Gadelha Rocha
This study aimed to evaluate the effect of diclofenac on minimum inhibitory concentrations of antifungals against planktonic cells and biofilms of Candida tropicalis. Susceptibility testing of planktonic cells was evaluated using the broth microdilution assay and checkerboard method. Biofilm formation by C. tropicalis in the presence of diclofenac, alone or in combination with antifungals, was also
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In vitro and in vivo evaluation of the antifungal activity of fluoxetine combined with antifungals against Candida albicans biofilms and oral candidiasis. Biofouling (IF 2.351) Pub Date : 2020-06-19 Liuliu Jiang,Linxia Zheng,K Airui Sun,Peng Zhou,Rui Xu,Jingyi Gu,Xin Wei
Candida albicans biofilms are responsible for oral candidiasis. Fluoxetine is a widely used antidepressant, with certain anti-Candida activities. The antifungal activity of fluoxetine combined with various antifungals against C. albicans biofilms and oral candidiasis was evaluated in this study. The morphological change in the inhibition of fluoxetine on C. albicans biofilms was observed using SEM
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2-Hydroxy-4-methoxybenzaldehyde from Hemidesmus indicus is antagonistic to Staphylococcus epidermidis biofilm formation. Biofouling (IF 2.351) Pub Date : 2020-06-26 Arunachalam Kannappan,Ravindran Durgadevi,Ramanathan Srinivasan,Ricardo José Lucas Lagoa,Issac Abraham Sybiya Vasantha Packiavathy,Shunmugiah Karutha Pandian,Arumugam Veera Ravi
Staphylococcus epidermidis (SE) is an opportunistic nosocomial pathogen that accounts for recalcitrant device-related infections worldwide. Owing to the growing interest in plants and their secondary metabolites targeting bacterial adhesion, this study was intended to uncover the anti-biofilm potential of Hemidesmus indicus and its major constituent 2-hydroxy-4-methoxybenzaldehyde (HMB) against SE
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Biofilms of Halobacterium salinarum as a tool for phenanthrene bioremediation. Biofouling (IF 2.351) Pub Date : 2020-06-25 Leonardo Gabriel Di Meglio,Juan Pablo Busalmen,César Nicolas Pegoraro,Débora Nercessian
The use of hyperhalophilic microorganisms is emerging as a sustainable alternative to clean hydrocarbon-polluted hypersaline water bodies. In line with this practice, this work reports on the ability of the archaeon Halobacterium salinarum to develop biofilms on a solid surface conditioned by the presence of phenanthrene crystals, which results in the removal of the contaminating compound. The cell
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A novel micellar formulation based on natural plant extracts enhances the efficacy of hydrogen peroxide against biofilms of Staphylococcus spp. and Pseudomonas aeruginosa. Biofouling (IF 2.351) Pub Date : 2020-06-26 Ojan Assadian,Beata Zatorska,Elisabeth Presterl,Magda Diab-El Schahawi
The antibacterial efficacy of hydrogen peroxide encapsulated in micelles (mH2O2) against biofilms was compared with that of hydrogen peroxide alone and of three commercially available aqueous biocides. The activity of mH2O2 on 24-h biofilms of reference strains of Staphylococcus spp. and Pseudomonas aeruginosa was tested in a static microtiter plate model. The biofilms were incubated with mH2O2 (17%
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Heterogeneity in the efficacy of dental chemical disinfectants on water-derived biofilms in vitro. Biofouling (IF 2.351) Pub Date : 2020-06-25 C Zemouri,J J de Soet,C M C Volgenant,W Crielaard,A M G A Laheij
Conditions in dental unit waterlines are favourable for biofilm growth and contamination of dental unit water. The aim of this study was to assess the effect of several chemical disinfectants on bacteria in a biofilm model. Water-derived biofilms were grown in a static biofilm model (Amsterdam Active Attachment model), using two growth media. Biofilms were challenged with Alpron/Bilpron, Anoxyl, Citrisil
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Human probiotic bacteria attenuate Pseudomonas aeruginosa biofilm and virulence by quorum-sensing inhibition. Biofouling (IF 2.351) Pub Date : 2020-06-23 Myriam Anabel Díaz,Silvia Nelina González,María Rosa Alberto,Mario Eduardo Arena
This work investigated chloroform extracts from culture supernatants of two human probiotic bacteria, Lactobacillus casei CRL 431 and Lactobacillus acidophilus CRL 730 for the production of virulence factors and quorum sensing (QS) interference against three Pseudomonas aeruginosa strains. Both extracts inhibited biofilm biomass (up to 50%), biofilm metabolic activity (up to 39%), the production of
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Proposal for a microcosm biofilm model for the study of vulvovaginal candidiasis. Biofouling (IF 2.351) Pub Date : 2020-07-03 Rossana de Aguiar Cordeiro,Ana Raquel Colares de Andrade,Fernando Victor Monteiro Portela,Lívia Maria Galdino Pereira,Santiago Gonçalves Bezerra Moura,Mônica Dantas Sampaio,Estefania Mota Araripe Pereira,Gláucia Morgana de Melo Guedes,Silviane Praciano Bandeira,Reginaldo Gonçalves de Lima-Neto,Vânia Maria Maciel Melo,Raimunda Sâmia Nogueira Brilhante,Débora Souza Collares Maia Castelo-Branco,Marcos
This study proposes a microcosm biofilm (MiB) model for the study of vulvovaginal candidiasis (VVC). Different conditions that mimic the vaginal environment were tested for MiB formation. The best growth conditions were obtained with samples incubated in vaginal fluid simulator medium pH 4.5 at 35 °C under a microaerophilic atmosphere. MiBs were evaluated for growth kinetics, fluconazole susceptibility
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