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Blocking drug efflux mechanisms facilitate genome engineering process in hypercellulolytic fungus, Penicillium funiculosum NCIM1228 Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-25 Anmoldeep Randhawa; Nandita Pasari; Tulika Sinha; Mayank Gupta; Anju M. Nair; Olusola A. Ogunyewo; Sandhya Verma; Praveen Kumar Verma; Syed Shams Yazdani
Penicillium funiculosum NCIM1228 is a non-model filamentous fungus that produces high-quality secretome for lignocellulosic biomass saccharification. Despite having desirable traits to be an industrial workhorse, P. funiculosum has been underestimated due to a lack of reliable genetic engineering tools. Tolerance towards common fungal antibiotics had been one of the major hindrances towards development
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Approaches to genetic tool development for rapid domestication of non-model microorganisms Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-25 Lauren A. Riley; Adam M. Guss
Non-model microorganisms often possess complex phenotypes that could be important for the future of biofuel and chemical production. They have received significant interest the last several years, but advancement is still slow due to the lack of a robust genetic toolbox in most organisms. Typically, “domestication” of a new non-model microorganism has been done on an ad hoc basis, and historically
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Four cellulose-active lytic polysaccharide monooxygenases from Cellulomonas species Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-23 James Li; Laleh Solhi; Ethan D. Goddard-Borger; Yann Mathieu; Warren W. Wakarchuk; Stephen G. Withers; Harry Brumer
The discovery of lytic polysaccharide monooxygenases (LPMOs) has fundamentally changed our understanding of microbial lignocellulose degradation. Cellulomonas bacteria have a rich history of study due to their ability to degrade recalcitrant cellulose, yet little is known about the predicted LPMOs that they encode from Auxiliary Activity Family 10 (AA10). Here, we present the comprehensive biochemical
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Unraveling the roles of the reductant and free copper ions in LPMO kinetics Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-21 Anton A. Stepnov; Zarah Forsberg; Morten Sørlie; Giang-Son Nguyen; Alexander Wentzel; Åsmund K. Røhr; Vincent G. H. Eijsink
Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze oxidative depolymerization of industrially relevant crystalline polysaccharides, such as cellulose, in a reaction that depends on an electron donor and O2 or H2O2. While it is well known that LPMOs can utilize a wide variety of electron donors, the variation in reported efficiencies of various LPMO-reductant combinations
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Lignin intermediates lead to phenyl acid formation and microbial community shifts in meso- and thermophilic batch reactors Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-20 Eva Maria Prem; Mira Mutschlechner; Blaz Stres; Paul Illmer; Andreas Otto Wagner
Lignin intermediates resulting from lignocellulose degradation have been suspected to hinder anaerobic mineralisation of organic materials to biogas. Phenyl acids like phenylacetate (PAA) are early detectable intermediates during anaerobic digestion (AD) of aromatic compounds. Studying the phenyl acid formation dynamics and concomitant microbial community shifts can help to understand the microbial
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Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-19 Hsiang-Yen Su; Hua-Ying Li; Cai-Yun Xie; Qiang Fei; Ke-Ke Cheng
Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasingly important as a sustainable replacement for traditional petroleum-based production processes depending on fossil resources. Therefore, we engineered an Enterobacter cloacae budC and ldhA double-deletion
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Comprehensive understanding of the effects of metallic cations on enzymatic hydrolysis of humic acid-pretreated waste wheat straw Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-19 Wei Tang; Xinxing Wu; Caoxing Huang; Zhe Ling; Chenhuan Lai; Qiang Yong
Humic acids (HA) have been used in biorefinery process due to its surfactant properties as an aid to the pretreatment of lignocellulose, with results indicating a positive effect on delignification. However, the HA remaining on the surface of the pretreated lignocellulose has also been shown to provide a negative effect on ensuing enzymatic digestibility. Hence, a strategy of complexing metallic cations
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Coculture with hemicellulose-fermenting microbes reverses inhibition of corn fiber solubilization by Clostridium thermocellum at elevated solids loadings Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-18 Dhananjay Beri; Christopher D. Herring; Sofie Blahova; Suresh Poudel; Richard J. Giannone; Robert L. Hettich; Lee R. Lynd
The cellulolytic thermophile Clostridium thermocellum is an important biocatalyst due to its ability to solubilize lignocellulosic feedstocks without the need for pretreatment or exogenous enzyme addition. At low concentrations of substrate, C. thermocellum can solubilize corn fiber > 95% in 5 days, but solubilization declines markedly at substrate concentrations higher than 20 g/L. This differs for
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Microbial fuel cell-assisted utilization of glycerol for succinate production by mutant of Actinobacillus succinogenes Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-15 Tianwen Zheng; Bin Xu; Yaliang Ji; Wenming Zhang; Fengxue Xin; Weiliang Dong; Ping Wei; Jiangfeng Ma; Min Jiang
The global production of glycerol is increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succinate-producing candidate, cannot grow on glycerol anaerobically, as it needs a terminal electron acceptor to maintain the balance of intracellular NADH and NAD+. Microbial fuel cell (MFC) has
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AP2/ERF and R2R3-MYB family transcription factors: potential associations between temperature stress and lipid metabolism in Auxenochlorella protothecoides Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-15 Guanlan Xing; Jinyu Li; Wenli Li; Sin Man Lam; Hongli Yuan; Guanghou Shui; Jinshui Yang
Both APETALA2/Ethylene Responsive Factor (AP2/ERF) superfamily and R2R3-MYB family were from one of the largest diverse families of transcription factors (TFs) in plants, and played important roles in plant development and responses to various stresses. However, no systematic analysis of these TFs had been conducted in the green algae A. protothecoides heretofore. Temperature was a critical factor
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Controlling of two destructive zooplanktonic predators in Chlorella mass culture with surfactants Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-14 Xiaobin Wen; Aoqi Zhang; Xiaoyan Zhu; Lin Liang; Yan Huo; Kaixuan Wang; Youzhi Yu; Yahong Geng; Yi Ding; Yeguang Li
Predatory flagellates and ciliates are two common bio-contaminants which frequently cause biomass losses in Chlorella mass culture. Efficient and targeted ways are required to control these contaminations in Chlorella mass cultivation aiming for biofuel production especially. Five surfactants were tested for its ability to control bio-contaminations in Chlorella culture. All five surfactants were able
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Analysis of the lipid extraction performance in a cascade process for Scenedesmus almeriensis biorefinery Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-14 I. Papachristou; S. Akaberi; A. Silve; E. Navarro-López; R. Wüstner; K. Leber; N. Nazarova; G. Müller; W. Frey
Microalgae have attracted considerable interest due to their ability to produce a wide range of valuable compounds. Pulsed Electric Fields (PEF) has been demonstrated to effectively disrupt the microalgae cells and facilitate intracellular extraction. To increase the commercial viability of microalgae, the entire biomass should be exploited with different products extracted and valorized according
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Innovations in anaerobic digestion: a model-based study Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-13 Karol Postawa; Jerzy Szczygieł; Marek Kułażyński
Increasing the efficiency of the biogas production process is possible by modifying the technological installations of the biogas plant. In this study, specific solutions based on a mathematical model that lead to favorable results were proposed. Three configurations were considered: classical anaerobic digestion (AD) and its two modifications, two-phase AD (TPAD) and autogenerative high-pressure digestion
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A rapid thioacidolysis method for biomass lignin composition and tricin analysis Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-11 Fang Chen; Chunliu Zhuo; Xirong Xiao; Thomas H. Pendergast; Katrien M. Devos
Biomass composition varies from plant to plant and greatly affects biomass utilization. Lignin is a heterogeneous phenolic polymer derived mainly from p-coumaryl, coniferyl, and sinapyl alcohols and makes up to 10–25% of lignocellulosic biomass. Recently, tricin, an O-methylated flavone, was identified as a lignin monomer in many grass species. Tricin may function as a nucleation site for lignification
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Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-09 Quan Wang; Kazuyuki Oshita; Masaki Takaoka
Recent studies of lipid extraction from microalgae have focused primarily on dewatered or dried samples, and the processes are simple with high lipid yield. Yet, the dewatering with drying step is energy intensive, which makes the energy input during the lipid production more than energy output from obtained lipid. Thus, exploring an extraction technique for just a thickened sample without the dewatering
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Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-09 Jeffrey P. Tingley; Kristin E. Low; Xiaohui Xing; D. Wade Abbott
The production of biofuels as an efficient source of renewable energy has received considerable attention due to increasing energy demands and regulatory incentives to reduce greenhouse gas emissions. Second-generation biofuel feedstocks, including agricultural crop residues generated on-farm during annual harvests, are abundant, inexpensive, and sustainable. Unlike first-generation feedstocks, which
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Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13 Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-08 Dexin Wang; Baek-Rock Oh; Sungbeom Lee; Dae-Hyuk Kim; Min-Ho Joe
Bacillus subtilis CS13 was previously isolated for 2,3-butanediol (2,3-BD) and poly-γ-glutamic acid (γ-PGA) co-production. When culturing this strain without L-glutamic acid in the medium, 2,3-BD is the main metabolic product. 2,3-BD is an important substance and fuel with applications in the chemical, food, and pharmaceutical industries. However, the yield and productivity for the B. subtilis strain
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Solid-state NMR of unlabeled plant cell walls: high-resolution structural analysis without isotopic enrichment Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-07 Wancheng Zhao; Alex Kirui; Fabien Deligey; Frederic Mentink-Vigier; Yihua Zhou; Baocai Zhang; Tuo Wang
Multidimensional solid-state nuclear magnetic resonance (ssNMR) spectroscopy has emerged as an indispensable technique for resolving polymer structure and intermolecular packing in primary and secondary plant cell walls. Isotope (13C) enrichment provides feasible sensitivity for measuring 2D/3D correlation spectra, but this time-consuming procedure and its associated expenses have restricted the application
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Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB) Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-07 Beatrice Mongili; Annalisa Abdel Azim; Silvia Fraterrigo Garofalo; Esperanza Batuecas; Angela Re; Sergio Bocchini; Debora Fino
Plastic plays a crucial role in everyday life of human living, nevertheless it represents an undeniable source of land and water pollution. Polyhydroxybutyrate (PHB) is a bio-based and biodegradable polyester, which can be naturally produced by microorganisms capable of converting and accumulating carbon as intracellular granules. Hence, PHB-producing strains stand out as an alternative source to fossil-derived
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Production of 10-methyl branched fatty acids in yeast Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-07 Hannah G. Blitzblau; Andrew L. Consiglio; Paulo Teixeira; Donald V. Crabtree; Shuyan Chen; Oliver Konzock; Gamuchirai Chifamba; Austin Su; Annapurna Kamineni; Kyle MacEwen; Maureen Hamilton; Vasiliki Tsakraklides; Jens Nielsen; Verena Siewers; A. Joe Shaw
Despite the environmental value of biobased lubricants, they account for less than 2% of global lubricant use due to poor thermo-oxidative stability arising from the presence of unsaturated double bonds. Methyl branched fatty acids (BFAs), particularly those with branching near the acyl-chain mid-point, are a high-performance alternative to existing vegetable oils because of their low melting temperature
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Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440 Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-07 Zhangyang Xu; Chunmei Pan; Xiaolu Li; Naijia Hao; Tong Zhang; Matthew J. Gaffrey; Yunqiao Pu; John R. Cort; Arthur J. Ragauskas; Wei-Jun Qian; Bin Yang
Efficient utilization of all available carbons from lignocellulosic biomass is critical for economic efficiency of a bioconversion process to produce renewable bioproducts. However, the metabolic responses that enable Pseudomonas putida to utilize mixed carbon sources to generate reducing power and polyhydroxyalkanoate (PHA) remain unclear. Previous research has mainly focused on different fermentation
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Membranes for bioethanol production by pervaporation Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-07 Ping Peng; Yongqiang Lan; Lun Liang; Kemeng Jia
Bioethanol as a renewable energy resource plays an important role in alleviating energy crisis and environmental protection. Pervaporation has achieved increasing attention because of its potential to be a useful way to separate ethanol from the biomass fermentation process. This overview of ethanol separation via pervaporation primarily concentrates on transport mechanisms, fabrication methods, and
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Novel ethanol production using biomass preprocessing to increase ethanol yield and reduce overall costs Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-07 Danielle Uchimura Pascoli; Azra Suko; Rick Gustafson; Heidi L. Gough; Renata Bura
Ethanol biorefineries need to lower their overall production costs to become economically feasible. Two strategies to achieve this are to reduce costs using cheaper feedstocks or to increase the ethanol production yield. Low-cost feedstocks usually have high non-structural components (NSC) content; therefore, a new process is necessary to accommodate these feedstocks and overcome the negative effects
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Modulating redox metabolism to improve isobutanol production in Shimwellia blattae Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-06 Miguel G. Acedos; Isabel de la Torre; Victoria E. Santos; Félix García-Ochoa; José L. García; Beatriz Galán
Isobutanol is a candidate to replace gasoline from fossil resources. This higher alcohol can be produced from sugars using genetically modified microorganisms. Shimwellia blattae (p424IbPSO) is a robust strain resistant to high concentration of isobutanol that can achieve a high production rate of this alcohol. Nevertheless, this strain, like most strains developed for isobutanol production, has some
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Enhanced methane production from cellulose using a two-stage process involving a bioelectrochemical system and a fixed film reactor Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-06 Kengo Sasaki; Daisuke Sasaki; Yota Tsuge; Masahiko Morita; Akihiko Kondo
It is desirable to improve the anaerobic digestion processes of recalcitrant materials, such as cellulose. Enhancement of methane (CH4) production from organic molecules was previously accomplished through coupling a bioelectrochemical system (BES); however, scaling-up BES-based production is difficult. Here, we developed a two-stage process consisting of a BES using low-cost and low-reactive carbon
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Chimeric cellobiohydrolase I expression, activity, and biochemical properties in three oleaginous yeast Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-06 Markus Alahuhta; Qi Xu; Eric P. Knoshaug; Wei Wang; Hui Wei; Antonella Amore; John O. Baker; Todd Vander Wall; Michael E. Himmel; Min Zhang
Consolidated bioprocessing using oleaginous yeast is a promising modality for the economic conversion of plant biomass to fuels and chemicals. However, yeast are not known to produce effective biomass degrading enzymes naturally and this trait is essential for efficient consolidated bioprocessing. We expressed a chimeric cellobiohydrolase I gene in three different oleaginous, industrially relevant
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Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-06 Mobolaji Felicia Adegboye; Omena Bernard Ojuederie; Paola M. Talia; Olubukola Oluranti Babalola
The issues of global warming, coupled with fossil fuel depletion, have undoubtedly led to renewed interest in other sources of commercial fuels. The search for renewable fuels has motivated research into the biological degradation of lignocellulosic biomass feedstock to produce biofuels such as bioethanol, biodiesel, and biohydrogen. The model strain for biofuel production needs the capability to utilize
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Biomass, lipid accumulation kinetics, and the transcriptome of heterotrophic oleaginous microalga Tetradesmus bernardii under different carbon and nitrogen sources Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-06 Baoyan Gao; Feifei Wang; Luodong Huang; Hui Liu; Yuming Zhong; Chengwu Zhang
Heterotrophic cultivation of microalgae has been proposed as a viable alternative method for novel high-value biomolecules, enriched biomass, and biofuel production because of their allowance of high cell density levels, as well as simple production technology. Tetradesmus bernardii, a newly isolated high-yielding oleaginous microalga under photoautotrophic conditions, is able to grow heterotrophically
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CRISPR-mediated multigene integration enables Shikimate pathway refactoring for enhanced 2-phenylethanol biosynthesis in Kluyveromyces marxianus Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-06 Mengwan Li; Xuye Lang; Marcos Moran Cabrera; Sawyer De Keyser; Xiyan Sun; Nancy Da Silva; Ian Wheeldon
2-phenylethanol (2-PE) is a rose-scented flavor and fragrance compound that is used in food, beverages, and personal care products. Compatibility with gasoline also makes it a potential biofuel or fuel additive. A biochemical process converting glucose or other fermentable sugars to 2-PE can potentially provide a more sustainable and economical production route than current methods that use chemical
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Genome-scale metabolic modeling underscores the potential of Cutaneotrichosporon oleaginosus ATCC 20509 as a cell factory for biofuel production Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-06 Nhung Pham; Maarten Reijnders; Maria Suarez-Diez; Bart Nijsse; Jan Springer; Gerrit Eggink; Peter J. Schaap
Cutaneotrichosporon oleaginosus ATCC 20509 is a fast-growing oleaginous basidiomycete yeast that is able to grow in a wide range of low-cost carbon sources including crude glycerol, a byproduct of biodiesel production. When glycerol is used as a carbon source, this yeast can accumulate more than 50% lipids (w/w) with high concentrations of mono-unsaturated fatty acids. To increase our understanding
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Enzymatic degradation of maize shoots: monitoring of chemical and physical changes reveals different saccharification behaviors Biotechnol. Biofuels (IF 4.815) Pub Date : 2021-01-05 Cécile Barron; Marie-Françoise Devaux; Loïc Foucat; Xavier Falourd; Rachelle Looten; Maud Joseph-Aime; Sylvie Durand; Estelle Bonnin; Catherine Lapierre; Luc Saulnier; Xavier Rouau; Fabienne Guillon
The recalcitrance of lignocellulosics to enzymatic saccharification has been related to many factors, including the tissue and molecular heterogeneity of the plant particles. The role of tissue heterogeneity generally assessed from plant sections is not easy to study on a large scale. In the present work, dry fractionation of ground maize shoot was performed to obtain particle fractions enriched in
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Enhanced xylitol production using non-detoxified xylose rich pre-hydrolysate from sugarcane bagasse by newly isolated Pichia fermentans Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-29 Ashish A. Prabhu; Ekkarin Bosakornranut; Yassin Amraoui; Deepti Agrawal; Frederic Coulon; Vivekanand Vivekanand; Vijay Kumar Thakur; Vinod Kumar
Integrated management of hemicellulosic fraction and its economical transformation to value-added products is the key driver towards sustainable lignocellulosic biorefineries. In this aspect, microbial cell factories are harnessed for the sustainable production of commercially viable biochemicals by valorising C5 and C6 sugars generated from agro-industrial waste. However, in the terrestrial ecosystem
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Homologous production, one-step purification, and proof of Na+ transport by the Rnf complex from Acetobacterium woodii, a model for acetogenic conversion of C1 substrates to biofuels Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-21 Anja Wiechmann; Dragan Trifunović; Sophie Klein; Volker Müller
Capture and storage of the energy carrier hydrogen as well as of the greenhouse gas carbon dioxide are two major problems that mankind faces currently. Chemical catalysts have been developed, but only recently a group of anaerobic bacteria that convert hydrogen and carbon dioxide to acetate, formate, or biofuels such as ethanol has come into focus, the acetogenic bacteria. These biocatalysts produce
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Consolidated bioprocessing of cellulose to itaconic acid by a co-culture of Trichoderma reesei and Ustilago maydis Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-14 Ivan Schlembach; Hamed Hosseinpour Tehrani; Lars M. Blank; Jochen Büchs; Nick Wierckx; Lars Regestein; Miriam A. Rosenbaum
Itaconic acid is a bio-derived platform chemical with uses ranging from polymer synthesis to biofuel production. The efficient conversion of cellulosic waste streams into itaconic acid could thus enable the sustainable production of a variety of substitutes for fossil oil based products. However, the realization of such a process is currently hindered by an expensive conversion of cellulose into fermentable
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Metabolic engineering of Aspergillus niger via ribonucleoprotein-based CRISPR–Cas9 system for succinic acid production from renewable biomass Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-14 Lei Yang; Mikkel Møller Henriksen; Rasmus Syrach Hansen; Mette Lübeck; Jesper Vang; Julie Egelund Andersen; Signe Bille; Peter Stephensen Lübeck
Succinic acid has great potential to be a new bio-based building block for deriving a number of value-added chemicals in industry. Bio-based succinic acid production from renewable biomass can provide a feasible approach to partially alleviate the dependence of global manufacturing on petroleum refinery. To improve the economics of biological processes, we attempted to explore possible solutions with
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Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-14 Chung-Jen Chiang; Yi-Jing Ho; Mu-Chen Hu; Yun-Peng Chao
The economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherichia coli. This issue was addressed by rewiring glycerol metabolism for redistribution of the metabolic flux. Key steps in
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Pretreatments for enhancing sewage sludge reduction and reuse in lipid production Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-14 Jiaxin Chen; Ji Li; Xiaolei Zhang; Zhaoyang Wu
Converting wastewater sludge to lipid is considered as one of the best strategies of sludge management. The current problem of lipid production from wastewater sludge is the low yield (0.10–0.16 g lipid/g dry sludge) due to the low availability of easily uptaken materials (such as soluble monosaccharide and oligosaccharide) in sludge to oleaginous microorganism (Rhodotorula glutinis, Trichosporon oleaginosus
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Cell-based and cell-free biocatalysis for the production of d-glucaric acid Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-10 Lu-Zhou Chen; Si-Ling Huang; Jin Hou; Xue-Ping Guo; Feng-Shan Wang; Ju-Zheng Sheng
d-Glucaric acid (GA) is a value-added chemical produced from biomass, and has potential applications as a versatile platform chemical, food additive, metal sequestering agent, and therapeutic agent. Marketed GA is currently produced chemically, but increasing demand is driving the search for eco-friendlier and more efficient production approaches. Cell-based production of GA represents an alternative
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Improved analysis of arabinoxylan-bound hydroxycinnamate conjugates in grass cell walls Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-10 Alexis Eugene; Catherine Lapierre; John Ralph
Arabinoxylan in grass cell walls is acylated to varying extents by ferulate and p-coumarate at the 5-hydroxy position of arabinosyl residues branching off the xylan backbone. Some of these hydroxycinnamate units may then become involved in cell wall radical coupling reactions, resulting in ether and other linkages amongst themselves or to monolignols or oligolignols, thereby crosslinking arabinoxylan
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Variation in sugarcane biomass composition and enzymatic saccharification of leaves, internodes and roots Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-09 Patrick J. Mason; Agnelo Furtado; Annelie Marquardt; Katrina Hodgson-Kratky; Nam V. Hoang; Frederik C. Botha; Gabriella Papa; Jenny C. Mortimer; Blake Simmons; Robert J. Henry
The composition of biomass determines its suitability for different applications within a biorefinery system. The proportion of the major biomass fractions (sugar, cellulose, hemicellulose and lignin) may vary in different sugarcane genotypes and growth environments and different parts of the plant. This study investigated the composition of mature and immature internodes, roots and mature leaves of
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Identification of a cytosine methyltransferase that improves transformation efficiency in Methylomonas sp. DH-1 Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-07 Jun Ren; Hyang-Mi Lee; Thi Duc Thai; Dokyun Na
Industrial biofuels and other value-added products can be produced from metabolically engineered microorganisms. Methylomonas sp. DH-1 is a candidate platform for bioconversion that uses methane as a carbon source. Although several genetic engineering techniques have been developed to work with Methylomonas sp. DH-1, the genetic manipulation of plasmids remains difficult because of the restriction-modification
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Physico-chemical assessment of torrefied Eurasian pinecones Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-07 Alok Dhaundiyal; Divine Atsu; Laszlo Toth
Biomass pre-treatment is gaining attention as a standalone process to improve the qualitative aspect of the lignocellulosic material. It has been gaining ground in the power station by replacing the coal with the pre-treated biomass. In this context, this paper enlightens the operating condition of carrying out the torrefaction so that the process can be made relatively more effective. The influence
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In silico screening and experimental analysis of family GH11 xylanases for applications under conditions of alkaline pH and high temperature Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-07 David Talens-Perales; Paloma Sánchez-Torres; Julia Marín-Navarro; Julio Polaina
Xylanases are one of the most extensively used enzymes for biomass digestion. However, in many instances, their use is limited by poor performance under the conditions of pH and temperature required by the industry. Therefore, the search for xylanases able to function efficiently at alkaline pH and high temperature is an important objective for different processes that use lignocellulosic substrates
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LACCASE14 is required for the deposition of guaiacyl lignin and affects cell wall digestibility in poplar Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-03 Shifei Qin; Chunfen Fan; Xiaohong Li; Yi Li; Jian Hu; Chaofeng Li; Keming Luo
The recalcitrance of lignocellulosic biomass provided technical and economic challenges in the current biomass conversion processes. Lignin is considered as a crucial recalcitrance component in biomass utilization. An in-depth understanding of lignin biosynthesis can provide clues to overcoming the recalcitrance. Laccases are believed to play a role in the oxidation of lignin monomers, leading to the
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Penicillium janthinellum NCIM1366 shows improved biomass hydrolysis and a larger number of CAZymes with higher induction levels over Trichoderma reesei RUT-C30 Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-12-01 AthiraRaj Sreeja-Raju; Meera Christopher; Prajeesh Kooloth-Valappil; Rajasree Kuni-Parambil; Digambar Vittal Gokhale; Meena Sankar; Amith Abraham; Ashok Pandey; Rajeev K. Sukumaran
Major cost of bioethanol is attributed to enzymes employed in biomass hydrolysis. Biomass hydrolyzing enzymes are predominantly produced from the hyper-cellulolytic mutant filamentous fungus Trichoderma reesei RUT-C30. Several decades of research have failed to provide an industrial grade organism other than T. reesei, capable of producing higher titers of an effective synergistic biomass hydrolyzing
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Characterization of an AA9 LPMO from Thielavia australiensis, TausLPMO9B, under industrially relevant lignocellulose saccharification conditions Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-30 F. Calderaro; M. Keser; M. Akeroyd; L. E. Bevers; V. G. H. Eijsink; A. Várnai; M. A. van den Berg
The discovery of lytic polysaccharide monooxygenases (LPMO) has changed our perspective on enzymatic degradation of plant biomass. Through an oxidative mechanism, these enzymes are able to cleave and depolymerize various polysaccharides, acting not only on crystalline substrates such as chitin and cellulose, but also on other polysaccharides, such as xyloglucan, glucomannan and starch. Despite their
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A thermostable bacterial lytic polysaccharide monooxygenase with high operational stability in a wide temperature range Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-30 Tina Rise Tuveng; Marianne Slang Jensen; Lasse Fredriksen; Gustav Vaaje-Kolstad; Vincent G. H. Eijsink; Zarah Forsberg
Lytic polysaccharide monooxygenases (LPMOs) are oxidative, copper-dependent enzymes that function as powerful tools in the turnover of various biomasses, including lignocellulosic plant biomass. While LPMOs are considered to be of great importance for biorefineries, little is known about industrial relevant properties such as the ability to operate at high temperatures. Here, we describe a thermostable
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Engineering prokaryotic regulator IrrE to enhance stress tolerance in budding yeast Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-30 Li Wang; Xin Wang; Zhi-Qiang He; Si-Jie Zhou; Li Xu; Xiao-Yu Tan; Tao Xu; Bing-Zhi Li; Ying-Jin Yuan
Stress tolerance is one of the important desired microbial traits for industrial bioprocesses, and global regulatory protein engineering is an efficient approach to improve strain tolerance. In our study, IrrE, a global regulatory protein from the prokaryotic organism Deinococcus radiodurans, was engineered to confer yeast improved tolerance to the inhibitors in lignocellulose hydrolysates or high
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Water-soluble chlorophyll-binding proteins from Brassica oleracea allow for stable photobiocatalytic oxidation of cellulose by a lytic polysaccharide monooxygenase Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-30 N. Dodge; D. A. Russo; B. M. Blossom; R. K. Singh; B. van Oort; R. Croce; M. J. Bjerrum; P. E. Jensen
Lytic polysaccharide monooxygenases (LPMOs) are indispensable redox enzymes used in industry for the saccharification of plant biomass. LPMO-driven cellulose oxidation can be enhanced considerably through photobiocatalysis using chlorophyll derivatives and light. Water soluble chlorophyll binding proteins (WSCPs) make it is possible to stabilize and solubilize chlorophyll in aqueous solution, allowing
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Metabolism, morphology and transcriptome analysis of oscillatory behavior of Clostridium butyricum during long-term continuous fermentation for 1,3-propanediol production Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-25 Jin-Jie Zhou; Jun-Tao Shen; Xiao-Li Wang; Ya-Qin Sun; Zhi-Long Xiu
Oscillation is a special cell behavior in microorganisms during continuous fermentation, which poses threats to the output stability for industrial productions of biofuels and biochemicals. In previous study, a spontaneous oscillatory behavior was observed in Clostridium butyricum-intensive microbial consortium in continuous fermentation for 1,3-propanediol (1,3-PDO) production from glycerol, which
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Promoting the co-utilisation of glucose and xylose in lignocellulosic ethanol fermentations using a data-driven feed-back controller Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-18 Pau Cabaneros Lopez; Isuru Abeykoon Udugama; Sune Tjalfe Thomsen; Christoph Bayer; Helena Junicke; Krist V. Gernaey
The diauxic growth of Saccharomyces cerevisiae on glucose and xylose during cellulose-to-ethanol processes extends the duration of the fermentation and reduces productivity. Despite the remarkable advances in strain engineering, the co-consumption of glucose and xylose is still limited due to catabolite repression. This work addresses this challenge by developing a closed-loop controller that is capable
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Correction to: Metabolic engineering of Yarrowia lipolytica for thermoresistance and enhanced erythritol productivity Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-17 Nan Wang; Ping Chi; Yawen Zou; Yirong Xu; Shuo Xu; Muhammad Bilal; Patrick Fickers; Hairong Cheng
An amendment to this paper has been published and can be accessed via the original article.
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Removal of fermentation inhibitors from pre-hydrolysis liquor using polystyrene divinylbenzene resin Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-12 Caoxing Huang; Yayue Zheng; Wenqian Lin; Yuxuan Shi; Guohong Huang; Qiang Yong
The presence of soluble lignin, furfural and hydroxymethylfurfural (HMF) in industrial pre-hydrolysis liquor (PHL) from the pulping process can inhibit its bioconversion into bioethanol and other biochemicals. Although various technologies have been developed to remove these inhibitors, certain amounts of sugars are also inevitably removed during the treatment process. Hence, polystyrene divinylbenzene
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ARTP/EMS-combined multiple mutagenesis efficiently improved production of raw starch-degrading enzymes in Penicillium oxalicum and characterization of the enzyme-hyperproducing mutant Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-11 Li-Sha Gu; Ming-Zhu Tan; Shi-Huan Li; Ting Zhang; Qi-Qiang Zhang; Cheng-Xi Li; Xue-Mei Luo; Jia-Xun Feng; Shuai Zhao
Application of raw starch-degrading enzymes (RSDEs) in starch processing for biofuel production can effectively reduce energy consumption and processing costs. RSDEs are generally produced by filamentous fungi, such as Penicillium oxalicum, but with very low yields, which seriously hampers industrialization of raw starch processing. Breeding assisted by random mutagenesis is an efficient way to improve
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Phylogenetics-based identification and characterization of a superior 2,3-butanediol dehydrogenase for Zymomonas mobilis expression Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-10 Venkataramanan Subramanian; Vladimir V. Lunin; Samuel J. Farmer; Markus Alahuhta; Kyle T. Moore; Angela Ho; Yogesh B. Chaudhari; Min Zhang; Michael E. Himmel; Stephen R. Decker
Zymomonas mobilis has recently been shown to be capable of producing the valuable platform biochemical, 2,3-butanediol (2,3-BDO). Despite this capability, the production of high titers of 2,3-BDO is restricted by several physiological parameters. One such bottleneck involves the conversion of acetoin to 2,3-BDO, a step catalyzed by 2,3-butanediol dehydrogenase (Bdh). Several Bdh enzymes have been successfully
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ATPase-based implementation of enforced ATP wasting in Saccharomyces cerevisiae for improved ethanol production Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-09 Ahmed Zahoor; Katrin Messerschmidt; Simon Boecker; Steffen Klamt
Enforced ATP wasting has been recognized as a promising metabolic engineering strategy to enhance the microbial production of metabolites that are coupled to ATP generation. It also appears to be a suitable approach to improve production of ethanol by Saccharomyces cerevisiae. In the present study, we constructed different S. cerevisiae strains with heterologous expression of genes of the ATP-hydrolyzing
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An automated workflow to screen alkene reductases using high-throughput thin layer chromatography Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-09 Brett M. Garabedian; Corey W. Meadows; Florence Mingardon; Joel M. Guenther; Tristan de Rond; Raya Abourjeily; Taek Soon Lee
Synthetic biology efforts often require high-throughput screening tools for enzyme engineering campaigns. While innovations in chromatographic and mass spectrometry-based techniques provide relevant structural information associated with enzyme activity, these approaches can require cost-intensive instrumentation and technical expertise not broadly available. Moreover, complex workflows and analysis
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Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-09 Heiko Babel; Jens O. Krömer
Isoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product. Adaptive laboratory evolution (ALE) is a promising method to address complex biological problems such as toxicity. Here we applied this method successfully to evolve E. coli towards higher
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Rapid optimisation of cellulolytic enzymes ratios in Saccharomyces cerevisiae using in vitro SCRaMbLE Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-03 Elizabeth L. I. Wightman; Heinrich Kroukamp; Isak S. Pretorius; Ian T. Paulsen; Helena K. M. Nevalainen
For the economic production of biofuels and other valuable products from lignocellulosic waste material, a consolidated bioprocessing (CBP) organism is required. With efficient fermentation capability and attractive industrial qualities, Saccharomyces cerevisiae is a preferred candidate and has been engineered to produce enzymes that hydrolyze cellulosic biomass. Efficient cellulose hydrolysis requires
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Optimization of carbon source efficiency for lipid production with the oleaginous yeast Saitozyma podzolica DSM 27192 applying automated continuous feeding Biotechnol. Biofuels (IF 4.815) Pub Date : 2020-11-02 Olga Gorte; Michaela Kugel; Katrin Ochsenreither
Biotechnologically produced microbial lipids are of interest as potential alternatives for crude and plant oils. Their lipid profile is similar to plant oils and can therefore be a substitute for the production of biofuels, additives for food and cosmetics industry as well as building blocks for oleochemicals. Commercial microbial lipids production, however, is still not profitable and research on
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