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Multi-omics analysis reveals novel loci and a candidate regulatory gene of unsaturated fatty acids in soybean (Glycine max (L.) Merr) Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-03-16 Xunchao Zhao, Yuhang Zhan, Kaiming Li, Yan Zhang, Changjun Zhou, Ming Yuan, Miao Liu, Yongguang Li, Peng Zuo, Yingpeng Han, Xue Zhao
Soybean is a major oil crop; the nutritional components of soybean oil are mainly controlled by unsaturated fatty acids (FA). Unsaturated FAs mainly include oleic acid (OA, 18:1), linoleic acid (LLA, 18:2), and linolenic acid (LNA, 18:3). The genetic architecture of unsaturated FAs in soybean seeds has not been fully elucidated, although many independent studies have been conducted. A 3 V multi-locus
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Carbon and energy balance of biotechnological glycolate production from microalgae in a pre-industrial scale flat panel photobioreactor Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-03-15 Heiko Wagner, Antonia Schad, Sonja Höhmann, Tim Arik Briol, Christian Wilhelm
Glycolate is produced by microalgae under photorespiratory conditions and has the potential for sustainable organic carbon production in biotechnology. This study explores the glycolate production balance in Chlamydomonas reinhardtii, using a custom-built 10-L flat panel bioreactor with sophisticated measurements of process factors such as nutrient supply, gassing, light absorption and mass balances
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Photoautotrophic cultivation of a Chlamydomonas reinhardtii mutant with zeaxanthin as the sole xanthophyll Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-03-14 Minjae Kim, Stefano Cazzaniga, Junhwan Jang, Matteo Pivato, Gueeda Kim, Matteo Ballottari, EonSeon Jin
Photosynthetic microalgae are known for their sustainable and eco-friendly potential to convert carbon dioxide into valuable products. Nevertheless, the challenge of self-shading due to high cell density has been identified as a drawback, hampering productivity in sustainable photoautotrophic mass cultivation. To address this issue, mutants with altered pigment composition have been proposed to allow
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A sustainable bioprocess to produce bacterial cellulose (BC) using waste streams from wine distilleries and the biodiesel industry: evaluation of BC for adsorption of phenolic compounds, dyes and metals Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-03-12 Erminta Tsouko, Sotirios Pilafidis, Konstantina Kourmentza, Helena I. Gomes, Giannis Sarris, Panagiota Koralli, Aristeidis Papagiannopoulos, Stergios Pispas, Dimitris Sarris
The main challenge for large-scale production of bacterial cellulose (BC) includes high production costs interlinked with raw materials, and low production rates. The valorization of renewable nutrient sources could improve the economic effectiveness of BC fermentation while their direct bioconversion into sustainable biopolymers addresses environmental pollution and/or resource depletion challenges
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Enhancing enzymatic saccharification yields of cellulose at high solid loadings by combining different LPMO activities Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-03-09 Camilla F. Angeltveit, Anikó Várnai, Vincent G. H. Eijsink, Svein J. Horn
The polysaccharides in lignocellulosic biomass hold potential for production of biofuels and biochemicals. However, achieving efficient conversion of this resource into fermentable sugars faces challenges, especially when operating at industrially relevant high solid loadings. While it is clear that combining classical hydrolytic enzymes and lytic polysaccharide monooxygenases (LPMOs) is necessary
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Kinetics-based development of two-stage continuous fermentation of 1,3-propanediol from crude glycerol by Clostridium butyricum Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-03-07 Xiao-Li Wang, Ya-Qin Sun, Duo-Tao Pan, Zhi-Long Xiu
Glycerol, as a by-product, mainly derives from the conversion of many crops to biodiesel, ethanol, and fatty ester. Its bioconversion to 1,3-propanediol (1,3-PDO) is an environmentally friendly method. Continuous fermentation has many striking merits over fed-batch and batch fermentation, such as high product concentration with easy feeding operation, long-term high productivity without frequent seed
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EnZolv delignification of cotton spinning mill waste and optimization of process parameters using response surface methodology (RSM) Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-03-07 Santhoshkumar Subramaniam, Kumutha Karunanandham, A. S. M. Raja, S. K. Shukla, Sivakumar Uthandi
EnZolv is a novel enzyme-based, eco-friendly biomass pretreatment process that has shown great potential in the field of textile engineering and biotechnology. It employs laccase from Hexagonia hirta MSF2 and 2% ethanol in the process of delignification. The process is designed to evaluate optimal conditions to remove lignin and other impurities from cotton spinning mill waste (CSMW), without compromising
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Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-03-05 Yuki Kasai, Satsuki Takagi, Shuhei Ota, Kotaro Ishii, Tsuyoshi Takeshita, Shigeyuki Kawano, Shigeaki Harayama
Previously, we isolated a mutant of Parachlorella kessleri named strain PK4 that accumulated higher concentrations of lipids than the wild-type strain. Resequencing of the PK4 genome identified mutations in three genes which may be associated with the high-lipid phenotype. The first gene, named CDMT1, encodes a protein with a calcium-dependent membrane association domain; the second gene, named DMAN1
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Enhanced bacterial cellulose production in Komagataeibacter sucrofermentans: impact of different PQQ-dependent dehydrogenase knockouts and ethanol supplementation Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-29 Pedro Montenegro-Silva, Tom Ellis, Fernando Dourado, Miguel Gama, Lucília Domingues
Bacterial cellulose (BC) is a biocompatible material with unique mechanical properties, thus holding a significant industrial potential. Despite many acetic acid bacteria (AAB) being BC overproducers, cost-effective production remains a challenge. The role of pyrroloquinoline quinone (PQQ)-dependent membrane dehydrogenases (mDH) is crucial in the metabolism of AAB since it links substrate incomplete
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Biocatalytic cascade to polysaccharide amination Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-27 Xuebin Feng, Siyi Hong, Hongbo Zhao, Thu V. Vuong, Emma R. Master
Chitin, the main form of aminated polysaccharide in nature, is a biocompatible, polycationic, and antimicrobial biopolymer used extensively in industrial processes. Despite the abundance of chitin, applications thereof are hampered by difficulties in feedstock harvesting and limited structural versatility. To address these problems, we proposed a two-step cascade employing carbohydrate oxidoreductases
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In silico design of multipoint mutants for enhanced performance of Thermomyces lanuginosus lipase for efficient biodiesel production Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-24 Jinsha Huang, Xiaoman Xie, Wanlin Zheng, Li Xu, Jinyong Yan, Ying Wu, Min Yang, Yunjun Yan
Biodiesel, an emerging sustainable and renewable clean energy, has garnered considerable attention as an alternative to fossil fuels. Although lipases are promising catalysts for biodiesel production, their efficiency in industrial-scale application still requires improvement. In this study, a novel strategy for multi-site mutagenesis in the binding pocket was developed via FuncLib (for mutant enzyme
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Identification of lipid synthesis genes in Schizochytrium sp. and their application in improving eicosapentaenoic acid synthesis in Yarrowia lipolytica Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-24 Yu-Lei Jia, Qing-Ming Zhang, Fei Du, Wen-Qian Yang, Zi-Xu Zhang, Ying-Shuang Xu, Wang Ma, Xiao-Man Sun, He Huang
Eicosapentaenoic acid (EPA) is widely used in the functional food and nutraceutical industries due to its important benefits to human health. Oleaginous microorganisms are considered a promising alternative resource for the production of EPA lipids. However, the storage of EPA in triglyceride (TG) becomes a key factor limiting its level. This study aimed to incorporate more EPA into TG storage through
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Bioprocess optimization for enhanced xylitol synthesis by new isolate Meyerozyma caribbica CP02 using rice straw Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-24 Saumya Singh, Shailendra Kumar Arya, Meena Krishania
The present work models the fermentation process parameters of the newly isolated, Meyerozyma caribbica CP02 for enhanced xylitol production and its fermentability study on rice straw hydrolysate. The study examined the impact of each of the process variables by one variable at a time optimization followed by statistical validation. Temperature of 32 °C, pH of 3.5, agitation of 200 rpm, 1.5% (v/v)
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A novel AA14 LPMO from Talaromyces rugulosus with bifunctional cellulolytic/hemicellulolytic activity boosted cellulose hydrolysis Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-23 Kaixiang Chen, Xu Zhao, Peiyu Zhang, Liangkun Long, Shaojun Ding
The recently discovered PcAA14A and B from white-rot basidiomycete Pycnoporus coccineus enriched our understanding of the oxidative degradation of xylan in fungi, however, the unusual mode of action of AA14 LPMOs has sparked controversy. The substrate specificity and functionality of AA14 LPMOs still remain enigmatic and need further investigation. In this study, a novel AA14 LPMO was characterized
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Identification of environment-insensitive genes for oil content by combination of transcriptome and genome-wide association analysis in rapeseed Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-22 Min Yao, Dan He, Wen Li, Xinghua Xiong, Xin He, Zhongsong Liu, Chunyun Guan, Lunwen Qian
The primary objective of rapeseed breeding is to enhance oil content, which is predominantly influenced by environmental factors. However, the molecular mechanisms underlying the impact of these environmental factors on oil accumulation remain inadequately elucidated. In this study, we used transcriptome data from two higher (HOC) and two lower oil content (LOC) inbred lines at 35 days after pollination
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Low CO2 concentration, a key environmental factor for developing plateau adapted rapeseed Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-21 Sha Liu, Lin Tang, Jingyan Fu, Caixia Zhao, Ying Zhang, Meng Yin, Maolin Wang, Rui Wang, Yun Zhao
Photosynthesis is a fundamental process that underlies the formation of crop yield, wherein light serves as the driving force and carbon dioxide (CO2) as the raw material. These two factors have a direct influence on the progress and efficiency of photosynthesis in crops. Rapeseed is one of the four major oilseed crops worldwide. Plateau rapeseed has now become a research hotspot. However, the lack
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Biosynthesis of melatonin from l-tryptophan by an engineered microbial cell factory Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-18 Lijuan Wang, Yongdong Deng, Jianjie Gao, Bo Wang, Hongjuan Han, Zhenjun Li, Wenhui Zhang, Yu Wang, Xiaoyan Fu, Rihe Peng, Quanhong Yao, Yongsheng Tian, Jing Xu
The demand for melatonin is increasing due to its health-promoting bioactivities such as antioxidant and sleep benefits. Although melatonin is present in various organisms, its low content and high extraction cost make it unsustainable. Biosynthesis is a promising alternative method for melatonin production. However, the ectopic production of melatonin in microorganisms is very difficult due to the
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Cultivation of microalgae–bacteria consortium by waste gas–waste water to achieve CO2 fixation, wastewater purification and bioproducts production Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-15 Wenwen Kong, Jia Kong, Shuo Feng, TianTian Yang, Lianfei Xu, Boxiong Shen, Yonghong Bi, Honghong Lyu
The cultivation of microalgae and microalgae–bacteria consortia provide a potential efficient strategy to fix CO2 from waste gas, treat wastewater and produce value-added products subsequently. This paper reviews recent developments in CO2 fixation and wastewater treatment by single microalgae, mixed microalgae and microalgae–bacteria consortia, as well as compares and summarizes the differences in
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Lignin bioconversion based on genome mining for ligninolytic genes in Erwinia billingiae QL-Z3 Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-15 Shuting Zhao, Dongtao Deng, Tianzheng Wan, Jie Feng, Lei Deng, Qianyi Tian, Jiayu Wang, Umm E. Aiman, Balym Mukhaddi, Xiaofeng Hu, Shaolin Chen, Ling Qiu, Lili Huang, Yahong Wei
Bioconversion of plant biomass into biofuels and bio-products produces large amounts of lignin. The aromatic biopolymers need to be degraded before being converted into value-added bio-products. Microbes can be environment-friendly and efficiently degrade lignin. Compared to fungi, bacteria have some advantages in lignin degradation, including broad tolerance to pH, temperature, and oxygen and the
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Genome-scale transcriptional activation by non-homologous end joining-mediated integration in Yarrowia lipolytica Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-15 Xiaoqin Liu, Jingyu Deng, Jinhong Zhang, Zhiyong Cui, Qingsheng Qi, Jin Hou
Genome-scale screening can be applied to efficiently mine for unknown genes with phenotypes of interest or special functions. It is also useful to identify new targets for engineering desirable properties of cell factories. Here, we designed a new approach for genome-scale transcription activation using non-homologous end joining (NHEJ)-mediated integration in Yarrowia lipolytica. We utilized this
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Marine cyanobacterial biomass is an efficient feedstock for fungal bioprocesses Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-13 Jai Kumar Gupta, Kavish K. Jain, Mehak Kaushal, Daniel J. Upton, Manish Joshi, Piyush Pachauri, A. Jamie Wood, Syed Shams Yazdani, Shireesh Srivastava
Marine cyanobacteria offer many sustainability advantages, such as the ability to fix atmospheric CO2, very fast growth and no dependence on freshwater for culture. Cyanobacterial biomass is a rich source of sugars and proteins, two essential nutrients for culturing any heterotroph. However, no previous study has evaluated their application as a feedstock for fungal bioprocesses. In this work, we cultured
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An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-11 Jiaxin Zhang, Kehang Li, Yu Sun, Cheng Yao, Weifeng Liu, Hong Liu, Yaohua Zhong
The CRISPR/Cas9 technology is being employed as a convenient tool for genetic engineering of the industrially important filamentous fungus Trichoderma reesei. However, multiplex gene editing is still constrained by the sgRNA processing capability, hindering strain improvement of T. reesei for the production of lignocellulose-degrading enzymes and recombinant proteins. Here, a CRISPR/Cas9 system based
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Calcium signaling positively regulates cellulase translation and secretion in a Clr-2-overexpressing, catabolically derepressed strain of Penicillium funiculosum Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-09 Anmoldeep Randhawa, Olusola A. Ogunyewo, Kamran Jawed, Syed Shams Yazdani
Low-cost cellulase production is vital to sustainable second-generation biorefineries. The catabolically derepressed strain of Penicillium funiculosum NCIM1228 (PfMig188 or ∆Mig1) secretes a superior set of cellulolytic enzymes, that are most suitable for 2G biorefineries. At a 3% (w/w) load, the ∆Mig1 secretome can release > 80% of fermentable sugars from lignocellulose at a 15% (w/v) biomass load
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Oxygenation influences xylose fermentation and gene expression in the yeast genera Spathaspora and Scheffersomyces Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-07 Katharina O. Barros, Megan Mader, David J. Krause, Jasmyn Pangilinan, Bill Andreopoulos, Anna Lipzen, Stephen J. Mondo, Igor V. Grigoriev, Carlos A. Rosa, Trey K. Sato, Chris Todd Hittinger
Cost-effective production of biofuels from lignocellulose requires the fermentation of d-xylose. Many yeast species within and closely related to the genera Spathaspora and Scheffersomyces (both of the order Serinales) natively assimilate and ferment xylose. Other species consume xylose inefficiently, leading to extracellular accumulation of xylitol. Xylitol excretion is thought to be due to the different
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Assessing the role of redox partners in TthLPMO9G and its mutants: focus on H2O2 production and interaction with cellulose Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-01 Koar Chorozian, Anthi Karnaouri, Nefeli Georgaki-Kondyli, Antonis Karantonis, Evangelos Topakas
The field of enzymology has been profoundly transformed by the discovery of lytic polysaccharide monooxygenases (LPMOs). LPMOs hold a unique role in the natural breakdown of recalcitrant polymers like cellulose and chitin. They are characterized by a “histidine brace” in their active site, known to operate via an O2/H2O2 mechanism and require an electron source for catalytic activity. Although significant
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Cumulative expression of heterologous XlnR regulatory modules and AraRA731V in Penicillium oxalicum enhances saccharification efficiency of corn stover and corn fiber Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-02-01 Chengqiang Xia, Xiaoyu Qi, Xin Song
Penicillium oxalicum engineered strain DB2 and its mutant strains with multiple regulatory modules were constructed. Mutant strain RE-4-2 with two regulatory modules showed a significant increase in the reducing sugar released from corn stover and corn fiber as well as in the conversion of cellulose than DB2. RE-5-2 with three regulatory modules showed a further increase in reducing sugar released
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Chromosome-level assembly and analysis of Camelina neglecta: a novel diploid model for Camelina biotechnology research Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-31 Shuo Wang, Rostislav Y. Blume, Zhi-Wei Zhou, Shaoping Lu, Tara J. Nazarenus, Yaroslav B. Blume, Weibo Xie, Edgar B. Cahoon, Ling-Ling Chen, Liang Guo
Camelina neglecta is a new diploid Brassicaceae species, which has great research value because of its close relationship with the hexaploid oilseed crop Camelina sativa. Here, we report a chromosome-level assembly of C. neglecta with a total length of 210 Mb. By adopting PacBio sequencing and Hi-C technology, the C. neglecta genome was assembled into 6 chromosomes with scaffold N50 of 29.62 Mb. C
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Knockout of a PLD gene in Schizochytrium limacinum SR21 enhances docosahexaenoic acid accumulation by modulation of the phospholipid profile Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-30 Yiting Zhang, Xiaowen Cui, Shuizhi Lin, Tao Lu, Hao Li, Yinghua Lu, Mingfeng Cao, Xihuang Lin, Xueping Ling
The hydrolysis and transphosphatidylation of phospholipase D (PLD) play important roles in the interconversion of phospholipids (PLs), which has been shown to profoundly impact lipid metabolism in plants. In this study, the effect of the PLD1 gene of Schizochytrium limacinum SR21 (S. limacinum SR21) on lipid metabolism was investigated. PLD1 knockout had little impact on cell growth and lipid production
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Extracellular vesicles involved in growth regulation and metabolic modulation in Haematococcus pluvialis Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-28 Qunju Hu, Zhangli Hu, Xiaojun Yan, Jun Lu, Chaogang Wang
Microalgae-derived extracellular vesicles (EVs), which transfer their cargos to the extracellular environment to affect recipient cells, play important roles in microalgal growth and environmental adaptation. And, they are also considered as sustainable and renewable bioresources of delivery nanocarrier for bioactive molecules and/or artificial drug molecules. However, their molecular composition and
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Lipid membrane remodeling and metabolic response during isobutanol and ethanol exposure in Zymomonas mobilis Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-28 Julio Rivera Vazquez, Edna Trujillo, Jonathan Williams, Fukang She, Fitsum Getahun, Melanie M. Callaghan, Joshua J. Coon, Daniel Amador-Noguez
Recent engineering efforts have targeted the ethanologenic bacterium Zymomonas mobilis for isobutanol production. However, significant hurdles remain due this organism’s vulnerability to isobutanol toxicity, adversely affecting its growth and productivity. The limited understanding of the physiological impacts of isobutanol on Z. mobilis constrains our ability to overcome these production barriers
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Isopropanol production via the thermophilic bioconversion of sugars and syngas using metabolically engineered Moorella thermoacetica Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-28 Junya Kato, Takeshi Matsuo, Kaisei Takemura, Setsu Kato, Tatsuya Fujii, Keisuke Wada, Yusuke Nakamichi, Masahiro Watanabe, Yoshiteru Aoi, Tomotake Morita, Katsuji Murakami, Yutaka Nakashimada
Isopropanol (IPA) is a commodity chemical used as a solvent or raw material for polymeric products, such as plastics. Currently, IPA production depends largely on high-CO2-emission petrochemical methods that are not sustainable. Therefore, alternative low-CO2 emission methods are required. IPA bioproduction using biomass or waste gas is a promising method. Moorella thermoacetica, a thermophilic acetogenic
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Low-temperature methanation of fermentation gas with Ni-based catalysts in a multicomponent system Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-28 Jie Yin, Zihui Yao, Qizhi Zhao, Shikun Cheng, Xuemei Wang, Zifu Li
A large amount of greenhouse gases, such as carbon dioxide and methane, are released during the production process of bioethanol and biogas. Converting CO2 into methane is a promising way of capturing CO2 and generating high-value gas. At present, CO2 methanation technology is still in the early stage. It requires high temperature (300–400 ℃) and pressure (> 1 MPa), leading to high cost and energy
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Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-28 Xintong Wang, Xianghong Meng, Yanlong Dong, Chunhua Song, Fengyang Sui, Xinxin Lu, Xiaoxue Mei, Yawen Fan, Yan Liu
The increasingly severe salinization of the aquatic environment has led to serious damage to the habitats of aquatic organisms. Benthic diatoms are commonly employed as indicator species for assessing water quality and serve as a reflection of the overall health of the aquatic ecosystem. Nitzschia palea is a common diatom found in freshwater, with high oil content, rapid reproductive rate, and it is
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Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-23 Min Wang, Xiaoxue Ye, Hongwen Bi, Zhongbao Shen
The development of microalgal biofuels is of significant importance in advancing the energy transition, alleviating food pressure, preserving the natural environment, and addressing climate change. Numerous countries and regions across the globe have conducted extensive research and strategic planning on microalgal bioenergy, investing significant funds and manpower into this field. However, the microalgae
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Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-22 Shuaili Chen, Zhanhao Yang, Ze Zhong, Shiqin Yu, Jingwen Zhou, Jianghua Li, Guocheng Du, Guoqiang Zhang
Classical directed evolution is a powerful approach for engineering biomolecules with improved or novel functions. However, it traditionally relies on labour- and time-intensive iterative cycles, due in part to the need for multiple molecular biology steps, including DNA transformation, and limited screening throughput. In this study, we present an ultrahigh throughput in vivo continuous directed evolution
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Sequential catalytic lignin valorization and bioethanol production: an integrated biorefinery strategy Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-20 Yilu Wu, Changsheng Su, Zicheng Liao, Gege Zhang, Yongjie Jiang, Yankun Wang, Changwei Zhang, Di Cai, Peiyong Qin, Tianwei Tan
The effective valorization of lignin and carbohydrates in lignocellulose matrix under the concept of biorefinery is a primary strategy to produce sustainable chemicals and fuels. Based on the reductive catalytic fractionation (RCF), lignin in lignocelluloses can be depolymerized into viscous oils, while the highly delignified pulps with high polysaccharides retention can be transformed into various
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Lignin impairs Cel7A degradation of in vitro lignified cellulose by impeding enzyme movement and not by acting as a sink Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-19 Zachary K. Haviland, Daguan Nong, Nerya Zexer, Ming Tien, Charles T. Anderson, William O. Hancock
Cellulose degradation by cellulases has been studied for decades due to the potential of using lignocellulosic biomass as a sustainable source of bioethanol. In plant cell walls, cellulose is bonded together and strengthened by the polyphenolic polymer, lignin. Because lignin is tightly linked to cellulose and is not digestible by cellulases, is thought to play a dominant role in limiting the efficient
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The adc1 knockout with proC overexpression in Synechocystis sp. PCC 6803 induces a diversion of acetyl-CoA to produce more polyhydroxybutyrate Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-13 Suthira Utharn, Saowarath Jantaro
Lack of nutrients, in particular nitrogen and phosphorus, has been known in the field to sense glutamate production via 2-oxoglutarate and subsequently accelerate carbon storage, including glycogen and polyhydroxybutyrate (PHB), in cyanobacteria, but a few studies have focused on arginine catabolism. In this study, we first time demonstrated that gene manipulation on proC and adc1, related to proline
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Heterologous expression of Arabidopsis laccase2, laccase4 and peroxidase52 driven under developing xylem specific promoter DX15 improves saccharification in populus Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-13 Yogesh K. Ahlawat, Ajaya K. Biswal, Sarahani Harun, Anne E. Harman-Ware, Crissa Doeppke, Nisha Sharma, Chandrashekhar P. Joshi, Bertrand B. Hankoua
Secondary cell wall holds considerable potential as it has gained immense momentum to replace the lignocellulosic feedstock into fuels. Lignin one of the components of secondary cell wall tightly holds the polysaccharides thereby enhancing the recalcitrance and complexity in the biomass. Laccases (LAC) and peroxidases (PRX) are the major phenyl-oxidases playing key functions during the polymerization
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Minimal aromatic aldehyde reduction (MARE) yeast platform for engineering vanillin production Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-06 Qiwen Mo, Jifeng Yuan
Vanillin represents one of the most widely used flavoring agents in the world. However, microbial synthesis of vanillin is hindered by the host native metabolism that could rapidly degrade vanillin to the byproducts. Here, we report that the industrial workhorse Saccharomyces cerevisiae was engineered by systematic deletion of oxidoreductases to improve the vanillin accumulation. Subsequently, we harnessed
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Bioprospecting CAZymes repertoire of Aspergillus fumigatus for eco-friendly value-added transformations of agro-forest biomass Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-03 Namrata Joshi, Jasneet Grewal, Lukasz Drewniak, Kumar Pranaw
Valorizing waste residues is crucial to reaching sustainable development goals and shifting from a linear fossil-based economy to a circular economy. Fungal cell factories, due to their versatility and robustness, are instrumental in driving the bio-transformation of waste residues. The present work isolated a potent strain, i.e., Aspergillus fumigatus (ZS_AF), from an ancient Złoty Stok gold mine
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Bacterial transformation of lignin: key enzymes and high-value products Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-03 Jinming Gu, Qing Qiu, Yue Yu, Xuejian Sun, Kejian Tian, Menghan Chang, Yibing Wang, Fenglin Zhang, Hongliang Huo
Lignin, a natural organic polymer that is recyclable and inexpensive, serves as one of the most abundant green resources in nature. With the increasing consumption of fossil fuels and the deterioration of the environment, the development and utilization of renewable resources have attracted considerable attention. Therefore, the effective and comprehensive utilization of lignin has become an important
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Salicylic acid enhances cell growth, fatty acid and astaxanthin production in heterotrophic Chromochloris zofingiensis without reactive oxygen species elevation Biotechnol. Biofuels (IF 6.3) Pub Date : 2024-01-03 Xinwei Zhang, Zhao Zhang, Yanmei Peng, Yushu Zhang, Qingyang Li, Dongzhe Sun
The induction of lipid and astaxanthin accumulation in microalgae is often achieved through abiotic stress. However, this approach usually leads to oxidative stress, which results in relatively low growth rate. Phytohormones, as important small molecule signaling substances, not only affect the growth and metabolism of microalgae but also influence the intracellular reactive oxygen species level. This
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Emission characteristics of cellulosic jet biofuel blend under laminar and turbulent combustion Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-12-19 Ziyu Liu, Zhichao Wang, Xiaoyi Yang
Alternative biofuels have the potential to reduce greenhouse gas emissions and particulate matter due to free of aromatics compared to traditional petroleum-based aviation fuel. The potential mitigating emission of hydrothermal-condensation-hydrotreating jet biofuel (HCHJ) derived from agriculture residue was investigated. The effects of aviation biofuel components, blend ratio and equivalent ratio
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Four ways of implementing robustness quantification in strain characterisation Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-12-19 Luca Torello Pianale, Fabio Caputo, Lisbeth Olsson
In industrial bioprocesses, microorganisms are generally selected based on performance, whereas robustness, i.e., the ability of a system to maintain a stable performance, has been overlooked due to the challenges in its quantification and implementation into routine experimental procedures. This work presents four ways of implementing robustness quantification during strain characterisation. One Saccharomyces
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Characterization of an efficient N-oxygenase from Saccharothrix sp. and its application in the synthesis of azomycin Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-12-16 Chuanle Fan, Fang Zhou, Wei Huang, Yi Xue, Chao Xu, Rubing Zhang, Mo Xian, Xinjun Feng
The nitro group constitutes a significant functional moiety within numerous valuable substances, such as nitroimidazoles, a class of antimicrobial drugs exhibiting broad spectrum activity. Conventional chemical methods for synthesizing nitro compounds suffer from harsh conditions, multiple steps, and environmental issues. Biocatalysis has emerged as a promising alternative to overcome these drawbacks
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Catalytic hydrolysis of agar using magnetic nanoparticles: optimization and characterization Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-12-13 Anoth Maharjan, Wonho Choi, Hee Taek Kim, Jung-Ho Park
Agar is used as a gelling agent that possesses a variety of biological properties; it consists of the polysaccharides agarose and porphyrin. In addition, the monomeric sugars generated after agar hydrolysis can be functionalized for use in biorefineries and biofuel production. The main objective of this study was to develop a sustainable agar hydrolysis process for bioethanol production using nanotechnology
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Development of highly efficient and specific base editors in Actinobacillus succinogenes for enhancing succinic acid production Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-12-12 Chunmei Chen, Pu Zheng, Pengcheng Chen, Dan Wu
The production of bio-succinic acid (SA) from renewable feedstocks is a promising and sustainable approach to mitigating the high carbon emissions associated with the current energy crisis. Actinobacillus succinogenes was recognized as one of the most promising SA producers; however, lack of genetic background and the scarcity of genetic manipulation tools hinder the improvement in A. succinogenes
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Integration of (S)-2,3-oxidosqualene enables E. coli to become Iron Man E. coli with improved overall tolerance Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-12-10 Wenjie Sun, Yun Chen, Mengkun Li, Syed Bilal Shah, Tianfu Wang, Jin Hou, Linquan Bai, Yan Feng, Zaigao Tan
While representing a model bacterium and one of the most used chassis in biomanufacturing, performance of Escherichia coli is often limited by severe stresses. A super-robust E. coli chassis that could efficiently tolerant multiple severe stresses is thus highly desirable. Sterols represent a featured composition that distinguishes eukaryotes from bacteria and all archaea, and play a critical role
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Metabolic engineering of a stable haploid strain derived from lignocellulosic inhibitor tolerant Saccharomyces cerevisiae natural isolate YB-2625 Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-12-06 Ronald E. Hector, Jeffrey A. Mertens, Nancy N. Nichols
Significant genetic diversity exists across Saccharomyces strains. Natural isolates and domesticated brewery and industrial strains are typically more robust than laboratory strains when challenged with inhibitory lignocellulosic hydrolysates. These strains also contain genes that are not present in lab strains and likely contribute to their superior inhibitor tolerance. However, many of these strains
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Lipid accumulation mechanism of Amphora coffeaeformis under nitrogen deprivation and its application as a feed additive in Carassius auratus aquaculture Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-12-06 Yulin Cui, Kang Wang, Xiuzhi Zhou, Chunxiao Meng, Zhengquan Gao
Amphora coffeaeformis, a unicellular diatom, can significantly accumulate lipids under nitrogen (N) limitation. However, the molecular mechanism underlying lipid accumulation in A. coffeaeformis remains unknown and its application development is lagging. This work analyzed the lipid composition of A. coffeaeformis under N deprivation and investigated its mechanism underlying lipid accumulation using
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Potential of acetic acid to restore methane production in anaerobic reactors critically intoxicated by ammonia as evidenced by metabolic and microbial monitoring Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-12-02 Sébastien Lemaigre, Patrick A. Gerin, Gilles Adam, Dominika Klimek, Xavier Goux, Malte Herold, Zuzana Frkova, Magdalena Calusinska, Philippe Delfosse
Biogas and biomethane production from the on-farm anaerobic digestion (AD) of animal manure and agri-food wastes could play a key role in transforming Europe’s energy system by mitigating its dependence on fossil fuels and tackling the climate crisis. Although ammonia is essential for microbial growth, it inhibits the AD process if present in high concentrations, especially under its free form, thus
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Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-11-29 William Woodruff, Narendra Naik Deshavath, Vionna Susanto, Christopher V. Rao, Vijay Singh
Oleaginous yeasts are a promising candidate for the sustainable conversion of lignocellulosic feedstocks into fuels and chemicals, but their growth on these substrates can be inhibited as a result of upstream pretreatment and enzymatic hydrolysis conditions. Previous studies indicate a high citrate buffer concentration during hydrolysis inhibits downstream cell growth and ethanol fermentation in Saccharomyces
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Installing xylose assimilation and cellodextrin phosphorolysis pathways in obese Yarrowia lipolytica facilitates cost-effective lipid production from lignocellulosic hydrolysates Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-11-29 Yiran Zhang, Moying Li, Rui Zhu, Yu Xin, Zitao Guo, Zhenghua Gu, Zhongpeng Guo, Liang Zhang
Yarrowia lipolytica, one of the most charming chassis cells in synthetic biology, is unable to use xylose and cellodextrins. Herein, we present work to tackle for the first time the engineering of Y. lipolytica to produce lipids from cellodextrins and xylose by employing rational and combinatorial strategies. This includes constructing a cellodextrin-phosphorolytic Y. lipolytica by overexpressing Neurospora
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High-throughput droplet microfluidics screening and genome sequencing analysis for improved amylase-producing Aspergillus oryzae Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-11-29 Qinghua Li, Jinchang Lu, Jingya Liu, Jianghua Li, Guoqiang Zhang, Guocheng Du, Jian Chen
The exceptional protein secretion capacity, intricate post-translational modification processes, and inherent safety features of A. oryzae make it a promising expression system. However, heterologous protein expression levels of existing A. oryzae species cannot meet the requirement for industrial-scale production. Therefore, establishing an efficient screening technology is significant for the development
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Integrated microbiology and metabolomic analysis reveal the improvement of rice straw silage quality by inoculation of Lactobacillus brevis Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-11-28 Yu Sun, Qinglong Sun, Yunmeng Tang, Qingyang Li, Chunjie Tian, Haixia Sun
Ensiling technology holds promise for preserving and providing high-quality forage. However, the preservation of rice straw poses challenges due to its high lignocellulosic content and low water-soluble carbohydrate levels. Developing highly effective lactic acid bacteria (LAB) for rice straw silage remains a priority. This study evaluated the impact of three LAB strains, Lactobacillus brevis R33 (Lac33)
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Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-11-28 Jan Struckmann Poulsen, Williane Vieira Macêdo, Torben Bonde, Jeppe Lund Nielsen
The biogas produced through anaerobic digestion (AD) of renewable feedstocks is one of the promising alternatives to replace fossil-derived energy. Even though lignocellulosic biomass is the most abundant biomass on earth, only a small fraction is being used towards resources recovery, leaving a great potential unexploited. In this study, the combination of state-of-art genomic techniques and engineered
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Discovery of novel alkaline-tolerant xylanases from fecal microbiota of dairy cows Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-11-27 Xiaoling Zhang, Qin Miao, Bingling Tang, Ivan Mijakovic, Xiao-Jun Ji, Lingbo Qu, Yongjun Wei
Xylo-oligosaccharides (XOS) are considered as a promising type of prebiotics that can be used in foods, feeds, and healthcare products. Xylanases play a key role in the production of XOS from xylan. In this study, we conducted a metagenomic analysis of the fecal microbiota from dairy cows fed with different types of fodders. Despite the diversity in their diets, the main phyla observed in all fecal
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In situ adsorption of itaconic acid from fermentations of Ustilago cynodontis improves bioprocess efficiency Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-11-26 Johannes Pastoors, Alexander Deitert, Carina Michel, Karsten Günster, Maurice Finger, Jordy Hofstede, Jeff Deischter, Andreas Biselli, Jörn Viell, Regina Palkovits, Andreas Jupke, Jochen Büchs
Reducing the costs of biorefinery processes is a crucial step in replacing petrochemical products by sustainable, biotechnological alternatives. Substrate costs and downstream processing present large potential for improvement of cost efficiency. The implementation of in situ adsorption as an energy-efficient product recovery method can reduce costs in both areas. While selective product separation
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Harnessing redox proteomics to study metabolic regulation and stress response in lignin-fed Rhodococci Biotechnol. Biofuels (IF 6.3) Pub Date : 2023-11-20 Xiaolu Li, Austin Gluth, Song Feng, Wei-Jun Qian, Bin Yang
Rhodococci are studied for their bacterial ligninolytic capabilities and proclivity to accumulate lipids. Lignin utilization is a resource intensive process requiring a variety of redox active enzymes and cofactors for degradation as well as defense against the resulting toxic byproducts and oxidative conditions. Studying enzyme expression and regulation between carbon sources will help decode the