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TNFR1 mediates heterogeneity in single-cell NF-κB activation iScience (IF 5.8) Pub Date : 2024-03-11 Chieh-Teng Cheng, Jye-Chian Hsiao, Alexander Hoffmann, Hsiung-Lin Tu
NF-κB is a key regulator in immune signaling and is known to exhibit a digital activation pattern. Yet the molecular basis underlying the heterogeneity in NF-κB activation at single-cell level is not entirely understood. Here, we show that NF-κB activation in single cells is largely regulated by intrinsic differences at the receptor level. Using the genome editing and time-lapse imaging, we directly
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Resilient Design in Nuclear Energy: Critical Lessons from a Cross-Disciplinary Analysis of the Fukushima Dai-ichi Nuclear Accident iScience (IF 5.8) Pub Date : 2024-03-11 Ali Ayoub, Haruko M. Wainwright, Giovanni Sansavini, Randall Gauntt, Kimiaki Saito
This paper presents a multidisciplinary analysis of the Fukushima Dai-ichi Nuclear Power Plant accident. Along with the latest observations and simulation studies, we synthesize the time-series and event progressions during the accident across multiple disciplines, including in-plant physics and engineering systems, operators’ actions, emergency responses, meteorology, radionuclide release and transport
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Combined Electrochemical and Spectroscopic Investigations of Carbonate-Mediated Water Oxidation to Peroxide iScience (IF 5.8) Pub Date : 2024-03-11 Hossein Bemana, Nikolay Kornienko
The development of electrosynthetic technologies for HO production is appealing from a sustainability perspective. The use of carbonate species as mediators in water oxidation to peroxide has emerged as a viable route to do so but still many questions remain about the mechanism that must be addressed. To this end, this work combines electrochemical and spectroscopic methods to investigate reaction
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High-sensitivity, ultrawide linear range, antibacterial textile pressure sensor based on chitosan/MXene hierarchical architecture iScience (IF 5.8) Pub Date : 2024-03-11 Mengxi Gu, Xuan Zhou, Jienan Shen, Ruibin Xie, Yuhan Su, Junxue Gao, Binzhe Zhao, Jie Li, Yingjie Duan, Zhixun Wang, Yougen Hu, Guoqiang Gu, Lei Wang, Lei Wei, Chunlei Yang, Ming Chen
It is still a great challenge for the flexible piezoresistive pressure sensors to simultaneously achieve wide linearity and high sensitivity. Herein, we propose a high-performance textile pressure sensor based on chitosan (CTS)/MXene fiber. The hierarchical ‘point to line’ architecture enables the pressure sensor with high sensitivity of 1.16 kPa over a ultrawide linear range of 1.5 MPa. Furthermore
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Structural neuroplasticity after sleep loss modifies behavior and requires neurexin and neuroligin iScience (IF 5.8) Pub Date : 2024-03-11 Mara H. Cowen, David M. Raizen, Michael P. Hart
Structural neuroplasticity (changes in the size, strength, number, and targets of synaptic connections) can be modified by sleep and sleep disruption. However, the causal relationships between genetic perturbations, sleep loss, neuroplasticity, and behavior remains unclear. The GABAergic DVB neuron undergoes structural plasticity in adult males in response to adolescent stress, which rewires synaptic
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The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis iScience (IF 5.8) Pub Date : 2024-03-11 Ziqi Wang, Tongtong Li, Dongkai Liu, Mengjing Li, Shangming Liu, Xiaochen Yu, Hanzhen Li, Hui Song, Wei Zhao, Zhaojian Liu, Xiangfeng Chen, Gang Lu, Zi-Jiang Chen, Tao Huang, Hongbin Liu
Spermiogenesis defines the final phase of male germ cell differentiation. While multiple deubiquitinating enzymes have been linked to spermiogenesis, the impacts of deubiquitination on spermiogenesis remain poorly characterized. Here, we investigated the function of UAF1 in mouse spermiogenesis. We selectively deleted in premeiotic germ cells using the knock-in mouse strain ( sKO), and found that is
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Plasmodium falciparum Alba6 exhibits DNase activity and participates in stress response iScience (IF 5.8) Pub Date : 2024-03-08 Shiladitya Nag, Chinmoy Banerjee, Manish Goyal, Asim Azhar Siddiqui, Debanjan Saha, Somnath Mazumder, Subhashis Debsharma, Saikat Pramanik, Shubhra Jyoti Saha, Rudranil De, Uday Bandyopadhyay
Alba domain proteins, owing to their functional plasticity, play a significant role in organisms. Here, we report an intrinsic DNase activity of PfAlba6 from , an etiological agent responsible for human malignant malaria. We identified that tyrosine28 plays a critical role in the Mg driven 5’-3’ DNase activity of PfAlba6. PfAlba6 cleaves both dsDNA as well as ssDNA. We also characterized PfAlba6-DNA
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Cellular Plasticity and Fate Determination in Gastric Carcinogenesis iScience (IF 5.8) Pub Date : 2024-03-08 Zheng He, Xiao-Hai Hu, Tian-Yu He, Ting-Ting Zhao
Cellular plasticity serves as a crucial biological phenomenon in humans, integral to tissue repair and maintenance of dynamic environmental homeostasis post-injury. However, dysregulated activation of this beneficial mechanism can pave the way for tumorigenesis and cancer progression. In this review, we synthesize recent advancements concerning the properties and roles of gastric epithelial cells,
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A Non-Viral DNA Delivery System Consisting of Multifunctional Chimaeric Peptide Fused with Zinc Finger Protein iScience (IF 5.8) Pub Date : 2024-03-08 Siyuan Yu, Haifeng Pan, Han Yang, Haoyun Zhuang, Haihui Yang, Xuan Yu, Shiyin Zhang, Mujin Fang, Tingdong Li, Shengxiang Ge, Ningshao Xia
Non-viral gene delivery systems have received sustained attention as a promising alternative to viral vectors for disease treatment and prevention in recent years. Numerous methods have been developed to enhance gene uptake and delivery in the cytoplasm, however, due to technical difficulties and delivery efficiency, these systems still face challenges in a range of biological applications, especially
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Eco-Evolutionary Experience and Behavioural Innovation in Interactions with Non-Native Species iScience (IF 5.8) Pub Date : 2024-03-08 Florian Ruland, Andreas A. Meltl, Muriel S. Neugebauer, Jonathan M. Jeschke
Behavioural changes play an important role for animals to cope with human-induced rapid environmental change such as biological invasions. The concept of eco-evolutionary experience (EEE) postulates that native species are more strongly impacted by non-native species the more these differ from species they have coevolved with. Also, EEE could influence the degree of innovation in new behaviours shown
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Multi-step validation of a deep learning-based system with visual explanations for optical diagnosis of polyps with advanced features iScience (IF 5.8) Pub Date : 2024-03-08 Qing-Wei Zhang, Zhengjie Zhang, Jianwei Xu, Zi-Hao Dai, Ran Zhao, Jian Huang, Hong Qiu, Zhao-Rong Tang, Bo Niu, Xun-Bing Zhang, Peng-Fei Wang, Mei Yang, Wan-Yin Deng, Yan-Sheng Lin, Suncheng Xiang, Zhi-Zheng Ge, Dahong Qian, Xiao-Bo Li
Artificial intelligence (AI) has been found to assist in optical differentiation of hyperplastic and adenomatous colorectal polyps. We investigated whether AI can improve the accuracy of endoscopists' optical diagnosis of polyps with advanced features. We introduced our AI system distinguishing polyps with advanced features with more than 0.870 of accuracies in the internal validation and the external
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Derived energy storage systems from Brayton cycle iScience (IF 5.8) Pub Date : 2024-03-08 Huan Guo, Yi Zhang, Yujie Xu, Xuezhi Zhou, Haisheng Chen
Various energy storage systems (ESS) can be derived from Brayton cycle, with the most representative being compressed air energy storage and pumped thermal electricity storage systems. Although some important studies on above ESS are reported, the topological structure behind those systems (i.e., derivations of the Brayton cycle) has not been studied, and the underlying thermodynamic ideas still need
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Intrinsic anticipatory motives in non-human primate food consumption behaviour iScience (IF 5.8) Pub Date : 2024-03-08 Judit Inkeller, Balázs Knakker, Péter Kovács, Balázs Lendvai, István Hernádi
Future-oriented behaviour is regarded as a cornerstone of human cognition. One key phenomenon through which future-orientation can be studied is the delay of gratification, when consumption of an immediate reward is withstanded to achieve a larger reward later. The delays used in animal delay of gratification paradigms are rather short to be considered relevant for studying human-like future-orientation
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Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency iScience (IF 5.8) Pub Date : 2024-03-08 Benjamin M. Lorton, Christopher Warren, Humaira Ilyas, Prithviraj Nandigrami, Subray Hegde, Sean Cahill, Stephanie M. Lehman, Jeffrey Shabanowitz, Donald F. Hunt, Andras Fiser, David Cowburn, David Shechter
Histone chaperones–structurally diverse, non-catalytic proteins enriched with acidic intrinsically disordered regions (IDRs)–protect histones from spurious nucleic acid interactions and guide their deposition into and out of nucleosomes. Despite their conservation and ubiquity, the function of the chaperone acidic IDRs remains unclear. Here, we show that the Npm2 and Nap1 acidic IDRs are substrates
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TRIF-IFN-I pathway in Helicobacter-induced gastric cancer in an accelerated murine disease model and patient biopsies iScience (IF 5.8) Pub Date : 2024-03-08 Prerna Bali, Ivonne Lozano-Pope, Jonathan Hernandez, Monica V. Estrada, Maripat Corr, Michael A. Turner, Michael Bouvet, Christopher Benner, Marygorret Obonyo
( infection is a known cause of many digestive diseases, including gastritis, peptic ulcers, and gastric cancer. However, the underlying mechanisms by which infection triggers these disorders are still not clearly understood. Gastric cancer is a slow progressing disease, which makes it difficult to study. We have developed an accelerated disease progression mouse model, which leverages mice deficient
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The Adaptor Protein 2 (AP2) complex modulates habituation and behavioral selection across multiple pathways and time windows iScience (IF 5.8) Pub Date : 2024-03-08 Rodrigo Zúñiga Mouret, Jordyn P. Greenbaum, Hannah M. Doll, Eliza M. Brody, Emma L. Iacobucci, Nicholas C. Roland, Roy C. Simamora, Ivan Ruiz, Rory Seymour, Leanne Ludwick, Jacob A. Krawitz, Antonia H. Groneberg, João C. Marques, Alexandre Laborde, Gokul Rajan, Filippo Del Bene, Michael B. Orger, Roshan A. Jain
Animals constantly integrate sensory information with prior experience to select behavioral responses appropriate to the current situation. Genetic factors supporting this behavioral flexibility are often disrupted in neuropsychiatric conditions, such as the autism-linked gene which supports acoustically-evoked habituation learning. encodes an AP2 endocytosis adaptor complex subunit, though its behavioral
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Disease phenotypic screening in neuron-glia cocultures identifies blockers of inflammatory neurodegeneration iScience (IF 5.8) Pub Date : 2024-03-08 Timothy J.Y. Birkle, Henriette M.G. Willems, John Skidmore, Guy C. Brown
Neuropathology is often mediated by interactions between neurons and glia that cannot be modelled by monocultures. However, cocultures are difficult to use and analyse for high-content screening. Here, we perform compound screening using primary neuron-glia cultures to model inflammatory neurodegeneration, live-cell stains, and automated classification of neurons, astrocytes or microglia using open-source
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The motive forces in DNA-enabled nanomachinery iScience (IF 5.8) Pub Date : 2024-03-08 Tao Zhang, Huajie Liu
Building machines that can augment or replace human efforts to accomplish complex tasks is one of central topics for humanity. Especially, nanomachines made of discrete numbers of molecular components can perform intended mechanical movements in a predetermined manner. Utilizing free energies of Watson−Crick basepairing, different types of DNA nanomachines have been invented to operate intended stepwise
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Exploring an accurate machine learning model to quickly estimate stability of diverse energetic materials iScience (IF 5.8) Pub Date : 2024-03-08 Qiaolin Gou, Jing Liu, Haoming Su, Yanzhi Guo, Jiayi Chen, Xueyan Zhao, Xuemei Pu
High energy and low sensitivity have been the focus of developing new energetic materials (EMs). However, there has been a lack of a quick and accurate method for evaluating the stability of diverse EMs. Here, we develop a machine learning prediction model with high accuracy for bond dissociation energy (BDE) of EMs. A reliable and representative BDE dataset of EMs is constructed by collecting 778
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Tracking SO2 plumes from the Tonga volcano eruption with multi-satellite observations iScience (IF 5.8) Pub Date : 2024-03-08 Congzi Xia, Cheng Liu, Zhaonan Cai, Hongyu Wu, Qingxiang Li, Meng Gao
The Hunga Tonga-Hunga Ha’apai (HTHH) volcano eruption received worldwide attention due to its magnitude and potential effects on environment and climate. However, the operational sulfur dioxide (SO) products mis-estimated SO emissions under volcanic conditions due to large uncertainties in the assumptions of SO plume altitude. That might have occurred in pervious volcanic eruptions and misled understanding
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“Heterogeneous subpopulations of GABAAR-responding neurons coexist across neuronal networks scales and developmental stages in health and disease” iScience (IF 5.8) Pub Date : 2024-03-08 I. Colombi, M. Rastogi, M. Parrini, M. Alberti, A. Potenzieri, M. Chelali, S. Rosati, M. Chiappalone, M. Nanni, A. Contestabile, L. Cancedda
GABA is the main inhibitory neurotransmitter in adults. Depolarizing GABA responses have been well characterized at neuronal-population level during typical neurodevelopment and partially in brain disorders. However, no investigation has specifically assessed whether a of cells with either depolarizing or hyperpolarizing/inhibitory GABAergic responses exists in animals in health/disease at diverse
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Cancer burden attributable to risk factors, 1990-2019: a comparative risk assessment iScience (IF 5.8) Pub Date : 2024-03-08 Junjie Hu, Hongliang Dong, Yiming Dong, Runxuan Zhou, Wilhem Teixeira, Xingxing He, Da-Wei Ye, Gang Ti
An up-to-date comprehensive assessment of the cancer burden attributable to risk factors is essential for cancer prevention. We analyzed the population attributable fraction (PAF) of cancer disability-adjusted life years (DALYs) attributable to 11 level 2 risk factors using data from the Global Burden and Disease Study (GBD) 2019. We highlighted that almost half of the cancer DALYs can be preventable
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GPT-4-powered analysis and prediction of selective catalytic reduction experiments through an effective chain-of-thought prompting strategy iScience (IF 5.8) Pub Date : 2024-03-07 Muyu Lu, Fengyu Gao, Xiaolong Tang, Linjiang Chen
This study explores the use of Large Language Models (LLMs) in interpreting and predicting experimental outcomes based on given experimental variables, leveraging the human-like reasoning and inference capabilities of LLMs, using selective catalytic reduction of NO with NH as a case study. We implement the Chain of Thought (CoT) concept to formulate logical steps for uncovering connections within the
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Phenotypic Switching as a Non-genetic Mechanism of Resistance Predicts Antibody Therapy Regimens iScience (IF 5.8) Pub Date : 2024-03-07 Jiawei Zhou, Can Liu, Yu Tang, Zhongbo Li, Yanguang Cao
Despite the specificity and effectiveness of antibody therapy, resistance to treatment remains a major barrier for their broad clinical applications. While genetic mutations are known critical, the impact of non-genetic mechanisms, such as epigenetic changes and phenotypic adaptations, on resistance to antibody-dependent cellular cytotoxicity (ADCC) is not fully understood. Our study investigated the
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Microstructure changes and miRNA–mRNA network in a developmental dysplasia of the hip rat model iScience (IF 5.8) Pub Date : 2024-03-07 Jiahui Liu, Yiyao Bao, Jiajie Fan, Wenhao Chen, Qiang Shu
MicroRNAs (miRNAs) interact with mRNAs in various pathophysiological processes. In developmental dysplasia of the hip (DDH), the miRNA–mRNA pairs affecting acetabular cartilage (AC) development remain unknown. We investigated dynamic microstructure changes and mRNA and miRNA expression profiles in the AC proliferative zone in a DDH rat model. Abnormal chondrocyte proliferation was observed, and several
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Systematic Omics Analysis Identifies CCR6 as a Therapeutic Target to Overcome Cancer Resistance to EGFR Inhibitors iScience (IF 5.8) Pub Date : 2024-03-07 Eun-Ji Kwon, Hyuk-Jin Cha, Haeseung Lee
Epidermal growth factor receptor inhibitors (EGFRi) have exhibited promising clinical outcomes in the treatment of various cancers. However, their widespread application has been limited by low patient eligibility and the emergence of resistance. Leveraging a multi-omics approach (>1000 cancer cell lines), we explored molecular signatures linked to EGFRi responsiveness and found that expression signatures
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High Paternal Homocysteine Causes Ventricular Septal Defects in Mouse Offspring iScience (IF 5.8) Pub Date : 2024-03-07 Lian Liu, Xuan Zhang, Hao-Ran Geng, Ya-Nan Qiao, Yong-Hao Gui, Jian-Yuan Zhao
Maternal hyperhomocysteinemia is widely considered as an independent risk of congenital heart disease (CHD). However, whether high paternal homocysteine causes CHD remains unknown. Here, we showed that increased homocysteine levels of male mice caused decreased sperm count, sperm motility defect and ventricular septal defect of the offspring. Moreover, high levels of paternal homocysteine decrease
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On-site detection and differentiation of African swine fever virus variants using an orthogonal CRISPR-Cas12b/Cas13a-based assay iScience (IF 5.8) Pub Date : 2024-03-07 Zhe Wang, Yu Wang, Ying Zhang, Guosong Qin, Wenbo Sun, Aiping Wang, Yanfang Wang, Gaiping Zhang, Jianguo Zhao
The African swine fever virus (ASFV) and its variants have induced substantial economic losses in China, prompting a critical need for efficient detection methods. Several PCR-based methods have been developed to discriminate between wild-type ASFV and gene-deleted variants. However, the requirement for sophisticated equipment and skilled operators limits their use in field settings. Here, we developed
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Temporal variables improve a spatiotemporal species distribution model for the non-native freshwater fish Candidia temminckii iScience (IF 5.8) Pub Date : 2024-03-06 Taichi Jibiki, Shinji Fukuda
Ecosystem conservation requires a deeper understanding of species-habitat relationships and population dynamics at a fine spatiotemporal resolution. We propose a new distribution modeling method based on a 5-year monthly survey that considers the temporal continuity of species distributions and physical habitat datasets by inputting continuous time-related variables. We employed random forests to relate
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Population genomics and morphological data bridge the centuries of cyanobacterial taxonomy along the continuum of Microcoleus species iScience (IF 5.8) Pub Date : 2024-03-06 Svatopluk Skoupý, Aleksandar Stanojković, Dale A. Casamatta, Callahan McGovern, Ana Martinović, Jiří Jaskowiec, Miriam Konderlová, Viktória Dodoková, Pavla Mikesková, Eva Jahodářová, Anne Jungblut, Hester van Schalkwyk, Petr Dvořák
The filamentous cyanobacterium is among the most important global primary producers, especially in hot and cold desert ecosystems. This taxon represents a continuum consisting of a minimum of 12 distinct species with varying levels of gene-flow and divergence. The notion of a species continuum is poorly understood in most lineages, but is especially challenging in cyanobacteria. Here we show that genomic
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Targeting SRSF2 Mutations in Leukemia with RKI-1447: A Strategy to Impair Cellular Division and Nuclear Structure iScience (IF 5.8) Pub Date : 2024-03-06 Minhua Su, Tom Fleisher, Inna Grosheva, Melanie Bokstad Horev, Malgorzata Olszewska, Camilla Ciolli Mattioli, Haim Barr, Alexander Plotnikov, Silvia Carvalho, Yoni Moskovich, Mark D. Minden, Noa Chapal-Ilani, Alexander Wainstein, Eirini P. Papapetrou, Nili Dezorella, Tao Cheng, Nathali Kaushansky, Benjamin Geiger, Liran I. Shlush
Spliceosome machinery mutations are common early mutations in myeloid malignancies, however effective targeted therapies against them are still lacking. In the current study, we used an in vitro high-throughput drug screen among four different isogenic cell lines and identified RKI-1447, a Rho-associated protein kinase inhibitor, as selective cytotoxic effector of mutant cells. RKI-1447 targeted Mut
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CTH-Net: A CNN and Transformer Hybrid Network for Skin Lesion Segmentation iScience (IF 5.8) Pub Date : 2024-03-06 Yuhan Ding, Zhenglin Yi, Jiatong Xiao, Minghui Hu, Yu Guo, Zhifang Liao, Yongjie Wang
Automatically and accurately segmenting skin lesions can be challenging, due to factors such as low contrast, and fuzzy boundaries. This paper proposes a hybrid encoder-decoder model (CTH-Net) based on CNN and Transformer, capitalizing on the advantages these approaches. We propose three modules for skin lesion segmentation and seamlessly connect them with a carefully designed model architecture. Better
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Transcriptome-informed identification and characterisation of Planococcus citri cis- and trans-isoprenyl diphosphate synthase genes iScience (IF 5.8) Pub Date : 2024-03-06 Mojca Juteršek, Iryna M. Gerasymenko, Marko Petek, Elisabeth Haumann, Sandra Vacas, Kalyani Kallam, Silvia Gianoglio, Vicente Navarro-Llopis, Michael Heethoff, Ismael Navarro Fuertes, Nicola Patron, Diego Orzáez, Kristina Gruden, Heribert Warzecha, Špela Baebler
Insect physiology and reproduction depend on several terpenoid compounds, whose biosynthesis is mainly unknown. One enigmatic group of insect monoterpenoids are mealybug sex pheromones, presumably resulting from the irregular coupling activity of unidentified isoprenyl diphosphate synthases (IDSs). Here, we performed a comprehensive search for IDS coding sequences of the pest mealybug . We queried
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Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusions iScience (IF 5.8) Pub Date : 2024-03-06 Martina Ramella, Lucrezia Maria Ribolla, Sara Surini, Kristyna Sala, Diletta Tonoli, Jean-Michel Cioni, Arpan Kumar Rai, Lucas Pelkmans, Ivan de Curtis
Plasma membrane-associated platforms (PMAPs) form at specific sites of plasma membrane by scaffolds including ERC1 and Liprin-α1. We identify a mechanism regulating PMAPs assembly, with consequences on motility/invasion. Silencing Ser/Thr kinase DYRK3 in invasive breast cancer cells inhibits their motility and invasive capacity. Similar effects on motility were observed by increasing DYRK3 levels,
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A tetravalent nanovaccine that inhibits growth of HPV-associated head and neck carcinoma via dendritic and T cell activation iScience (IF 5.8) Pub Date : 2024-03-06 Romano Josi, Daniel E. Speiser, Simone de Brot, Anne-Cathrine Vogt, Eva M. Sevick-Muraca, Genrich V. Tolstonog, Martin F. Bachmann, Mona O. Mohsen
The global incidence of human papillomavirus (HPV) associated head and neck carcinoma is on the rise, in response to this a tetravalent therapeutic vaccine named Qβ-HPVag was developed. This vaccine, utilizing virus-like particles (VLPs) loaded with toll-like receptor ligands and chemically coupled to four HPV16-derived peptides, demonstrated strong anti-tumor effects in a murine head and neck cancer
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Lethal combats in the forest among wild western gorillas iScience (IF 5.8) Pub Date : 2024-03-06 Hugo Potel, Frédéric Stéphane Niatou Singa, Chloé Cipolletta, Terence Fuh, Giulia Bardino, Emmanuel Konyal, Paolo Strampelli, Phillipp Henschel, Shelly Masi
Lethal inter-group encounters occur in many species because of sexual selection. While documented in mountain gorillas, they are absent in western gorillas as instead it is predicted by their higher feeding (frugivory) and mate competition (single- vs. multi-male groups). We investigate whether the injuries on three dead silverbacks and one adult female from four groups of western gorillas in Central
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Lumbar puncture increases Alzheimer’s disease biomarker levels in cerebrospinal fluid of rhesus monkeys iScience (IF 5.8) Pub Date : 2024-03-06 Jianglei Xu, Hao Li, Yingzhou Hu, Shihao Wu, Liping Wu, Xiaoguang Lei, Longbao Lv, Yi Lu, Jing Wu, Juanjuan Li, Bingyin Shi, Jiali Li, Christoph W. Turck, Wenchao Wang, Xintian Hu
Cerebrospinal fluid (CSF) samples are commonly collected via lumbar puncture (LP) in both clinical and research settings for measurement of biomarkers of Alzheimer’s disease (AD). To determine the effects of LP on CSF AD biomarkers, we collected CSF samples at seven different time points after a LP in rhesus monkeys. We find that amyloid-beta (Aβ) and Tau levels increased significantly on day 1, peaked
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Mild hypothermia promotes neuronal differentiation of human neural stem cells via RBM3-SOX11 signaling pathway iScience (IF 5.8) Pub Date : 2024-03-06 Yuxiao Ma, Zhenghui He, Jiangchang Wang, Ping Zheng, Zixuan Ma, Qian Liang, Qiao Zhang, Xiongfei Zhao, Jialin Huang, Weiji Weng, Jiyao Jiang, Junfeng Feng
Both therapeutic hypothermia and neural stem cells (NSCs) transplantation have shown promise in neuroprotection and neural repair after brain injury. However, the effects of therapeutic hypothermia on neuronal differentiation of NSCs are not elucidated. In this study, we aimed to investigate whether mild hypothermia promoted neuronal differentiation in cultured and transplanted human NSCs (hNSCs).
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RUVBL1 accelerates tongue squamous cell carcinoma by mediating C-Raf/MEK/ERK pathway iScience (IF 5.8) Pub Date : 2024-03-06 Xin-yu Zhang, Yang Liu, Qiong Rong, Ming-yue Qi, Hui Guo
RAF/MEK/ERK pathway is frequently activated in tumor. Therefore, this study will investigate the function of RUVBL1 (RAF-binding protein) in tongue squamous cell carcinoma (TSCC). Bioinformatics was performed to identify DE-mRNAs in TCGA-OSCC, GSE13601, and GSE34105 datasets. A total of 672 shared DE-mRNAs were identified in three datasets, and they are regulating metastasis, and angiogenesis. Patients
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Evolvability of cancer-associated genes under APOBEC3A/B selection iScience (IF 5.8) Pub Date : 2024-03-06 Joon-Hyun Song, Liliana M. Dávalos, Thomas MacCarthy, Mehdi Damaghi
Evolvability is an emergent hallmark of cancer that depends on intra-tumor heterogeneity and genetic variation. Mutations generated by APOBEC3 contribute to genetic variation and tumor evolvability. However, the influence of APOBEC3 on the evolvability of the genome and its differential impact on cancer genes versus non-cancer genes remains unclear. Analyzing over 40,000 human protein-coding transcripts
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Shared Genetic Architecture and Causal Relationship Between Liver and Heart Disease iScience (IF 5.8) Pub Date : 2024-03-06 Ziyi Fang, Sixiang Jia, Xuanting Mou, Zhe Li, Tianli Hu, Yiting Tu, Jianqiang Zhao, Tianlong Zhang, Wenting Lin, Yile Lu, Chao Feng, Shudong Xia
This study investigates the relationship and genetic mechanisms of liver and heart diseases, focusing on the Liver-Heart Axis (LHA) as a fundamental biological basis. Through GWAS analysis, we explore shared genes and pathways related to LHA. Shared genetic factors are found in 8 out of 20 pairs, indicating genetic correlations. The analysis reveals 53 loci with pleiotropic effects, including 8 loci
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Binder assisted graphene derivatives as lubricants in copper: improved tribological performance for industrial application iScience (IF 5.8) Pub Date : 2024-03-06 Changjie Huang, Su Zhao, Ruiqi Chen, Erik Johansson, Muhammad Aqeel, Uta Klement, Anna M. Andersson, Mamoun Taher, Vincenzo Palermo, Jinhua Sun
Originally derived from graphite, high-quality single-layer graphene is an excellent anti-wear and -friction additive in metal matrix. Here, the tribological performance of 3 different commercialized graphene derivatives (e.g., graphene oxide (GO), reduced graphene oxide (RGO), and graphene nanoplatelet (GNP)) as additives in a Cu matrix, were investigated from an industrial perspective. To increase
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A Nature-Based Closed-loop Wastewater Treatment System at Vehicle-Washing Facilities: From Linear to Circular Economy iScience (IF 5.8) Pub Date : 2024-03-06 Muhammad Afzal, Muhammad Arslan, Salman Younus, Jochen A. Müller, Muhammad Usman, Momina Yasin, Muhammad Aamer Mehmood, Tanveer Mehdi, Ejazul Islam, Muhammad Tauseef, Samina Iqbal
Pakistan, among the top five most water-stressed nations globally, grapples with water scarcity owing to inadequate treatment infrastructure and groundwater over-extraction. We demonstrate a successful nature-based closed-loop system to treat wastewater from urban vehicle-washing facilities, previously reliant on groundwater. An eco-friendly integrated system containing floating treatment wetlands
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The soil zones of Saskatchewan: Creating art to visualize the concept iScience (IF 5.8) Pub Date : 2024-03-06 K, e, n, , v, a, n, , R, e, e, s
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Electromagnetic and gravitational radiation of blazar OJ 287 iScience (IF 5.8) Pub Date : 2024-03-05 A.E. Volvach, L.N. Volvach, M.G. Larionov
Based on long-term monitoring data of the blazar, a new model for determining the parameters of this close binary supermassive black hole (SMBH) is proposed. The model uses the laws of celestial mechanics and results of harmonic analysis of observational data obtained mainly in the radio and optical wavelength ranges. Within the framework of the proposed model, the masses of SMBH companions, the values
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Laser-induced voltage of table salt for deep ultraviolet pulsed laser detection iScience (IF 5.8) Pub Date : 2024-03-05 Xuecong Liu, Kun Zhao, Xinyang Miao
To meet the requirements of fast response and simple process of deep ultraviolet (UV) pulsed laser detector, table salt (TS) was used as laser detection material in combination with a variable resistor to achieve single-pulse laser detection. Under the irradiation of a KrF excimer laser, the laser-induced voltage (LIV) of TS was influenced by the dynamic process of laser-induced plasma, and the whole
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Mathematical model calibrated to in vitro data predicts mechanisms of antiviral action of the influenza defective interfering particle “OP7” iScience (IF 5.8) Pub Date : 2024-03-05 Daniel Rüdiger, Julita Piasecka, Jan Küchler, Carolina Pontes, Tanja Laske, Sascha Y. Kupke, Udo Reichl
Defective interfering particles (DIPs) are regarded as potent broad-spectrum antivirals. We developed a mathematical model that describes intracellular co-infection dynamics of influenza standard virus (STV) and “OP7”, a new type of influenza DIP discovered recently. Based on experimental data from studies to calibrate the model and confirm its predictions, we deduce OP7’s mechanisms of interference
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Tropical fishery nutrient production depends on biomass-based management iScience (IF 5.8) Pub Date : 2024-03-05 Bryan P. Galligan, Timothy R. McClanahan
The need to enhance nutrient production from tropical ecosystems to feed the poor could potentially create a new framework for fisheries science and management. Early recommendations have included targeting small fishes and increasing the species richness of fish catches, which could represent a departure from more traditional approaches such as biomass-based management. To test these recommendations
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Health diagnosis and recuperation of aged Li-ion batteries with data analytics and equivalent circuit modeling iScience (IF 5.8) Pub Date : 2024-03-05 Riko I. Made, Jing Lin, Jintao Zhang, Yu Zhang, Lionel C.H. Moh, Zhaolin Liu, Ning Ding, Sing Yang Chiam, Edwin Khoo, Xuesong Yin, Guangyuan Wesley Zheng
Battery health assessment and recuperation play crucial roles in the utilization of second-life Li-ion batteries. However, due to ambiguous aging mechanisms, it is challenging to estimate battery health and devise an effective strategy for cell rejuvenation. This paper presents aging and reconditioning experiments of 62 commercial lithium iron phosphate cells, which allow us to use machine learning
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A novel Nav1.8-FLPo driver mouse for intersectional genetics to uncover the functional significance of primary sensory neuron diversity iScience (IF 5.8) Pub Date : 2024-03-05 Pascale Malapert, Guillaume Robert, Elena Brunet, Jean Chemin, Emmanuel Bourinet, Aziz Moqrich
The recent development of single-cell and single-nucleus RNA sequencing has highlighted the extraordinary diversity of dorsal root ganglia neurons. However, the few available genetic tools limit our understanding of the functional significance of this heterogeneity. We generated a new mouse line expressing the flippase recombinase from the locus. By crossing Na1.8 mice with the Advillin and RC::FL-hM3Dq
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SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway iScience (IF 5.8) Pub Date : 2024-03-05 Kai Yang, Ying-yi Luan, Shan Wang, You-sheng Yan, Yi-peng Wang, Jue Wu, Yong-qing Sun, Jing Zhang, Wen-qi Chen, Yu-lan Xiang, Ze-lu Li, Dong-liang Zhang, Cheng-hong Yin
Mesenchymal stem cell (MSC)-mediated coupling of osteogenesis and angiogenesis is a critical phenomenon in bone formation. Herein, we investigated the role and mechanism of SGMS1 in the osteogenic differentiation of MSCs and, in combination with osteogenesis and angiogenesis, to discover new therapeutic targets for skeletal dysplasia and bone defects. SGMS1 addition accelerated MSC osteogenic differentiation
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Comprehending phenotypic plasticity in cancer and evolution iScience (IF 5.8) Pub Date : 2024-03-05 Prakash Kulkarni, Ravi Salgia
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COVID-19 vaccine effectiveness against the Omicron variant of SARS-CoV-2 in multimorbidity: A territory-wide case-control study iScience (IF 5.8) Pub Date : 2024-03-04 Francisco Tsz Tsun Lai, Vincent Ka Chun Yan, Eric Yuk Fai Wan, Cheyenne I. Ying Chan, Cuiling Wei, Franco Wing Tak Cheng, Celine Sze Ling Chui, Xue Li, Carlos King Ho Wong, Ching Lung Cheung, Ian Chi Kei Wong, Esther Wai Yin Chan
Multimorbidity entails a higher risk of SARS-CoV-2 infection and COVID-19 complications. We examined vaccine effectiveness (VE) stratified by multimorbidity using a case-control study of territory-wide electronic health records in Hong Kong. Cases of infection (testing positive), hospitalization, and mortality were identified from January to March 2022. Controls were matched by age, sex, outpatient
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N6-methyladenosine-associated genetic variants in NECTIN2 and HPCAL1 are risk factors for abdominal aortic aneurysm iScience (IF 5.8) Pub Date : 2024-03-04 Tan Li, Yijun Wu, Jun Yang, Jingjing Jing, Chunyan Ma, Liping Sun
Although N6-methyladenosine (m6A) modification has been implicated in the pathogenesis of abdominal aortic aneurysm (AAA), the relationship between m6A-associated single nucleotide polymorphisms (m6A-SNPs) and AAA remains unknown. This study used integrative multi-omics analysis and clinical validation approaches to systematically identify potential m6A-SNPs connected with AAA risk. We found that rs6859
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Recent strides toward transforming lignin into plastics and aqueous electrolytes for flow batteries iScience (IF 5.8) Pub Date : 2024-03-04 Omar Y. Abdelaziz, Mariona Battestini Vives, Smita V. Mankar, Niklas Warlin, Tran Tam Nguyen, Baozhong Zhang, Christian P. Hulteberg, Amirreza Khataee
Lignin is an abundant polyaromatic polymer with a wide range of potential future uses. However, the conversion of lignin into valuable products comes at a cost, and medium- to high-value applications are thus appropriate. Two examples of these are polymers (e.g., as fibers, plasticizers, or additives) and flow batteries (e.g., as redox species). Both of these areas would benefit from lignin-derived
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MYC dependency in GLS1 and NAMPT is a therapeutic vulnerability in multiple myeloma iScience (IF 5.8) Pub Date : 2024-03-04 Lama Hasan Bou Issa, Léa Fléchon, William Laine, Aicha Ouelkdite, Silvia Gaggero, Adeline Cozzani, Remi Tilmont, Paul Chauvet, Nicolas Gower, Romanos Sklavenitis Pistofidis, Carine Brinster, Xavier Thuru, Yasmine Touil, Bruno Quesnel, Suman Mitra, Irene M. Ghobrial, Jérôme Kluza, Salomon Manier
Multiple myeloma (MM) is an incurable hematological malignancy in which MYC alterations contribute to the malignant phenotype. Nevertheless, MYC lacks therapeutic druggability. Here, we leveraged large-scale loss-of-function screens and conducted a small molecule screen to identify genes and pathways with enhanced essentiality correlated with expression. We reported a specific gene dependency in glutaminase
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Cortical brain organoid slices (cBOS) for the study of human neural cells in minimal networks iScience (IF 5.8) Pub Date : 2024-03-04 Laura Petersilie, Sonja Heiduschka, Joel S.E. Nelson, Louis A. Neu, Stephanie Le, Ruchika Anand, Karl W. Kafitz, Alessandro Prigione, Christine R. Rose
Brain organoids derived from human pluripotent stem cells are a promising tool for studying human neurodevelopment and related disorders. Here, we generated long-term cultures of cortical brain organoid slices (cBOS) grown at the air-liquid interphase from regionalized cortical organoids. We show that cBOS host mature neurons and astrocytes organized in complex architecture. Whole-cell patch-clamp
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Whole-genome bisulfite sequencing identifies stage- and subtype-specific DNA methylation signatures in pancreatic cancer iScience (IF 5.8) Pub Date : 2024-03-04 Sarah S. Wang, Madison L. Hall, EunJung Lee, Soon-Chan Kim, Neha Ramesh, Sang Hyub Lee, Jin-Young Jang, Ja-Lok Ku, Chang-Il Hwang
In pancreatic ductal adenocarcinoma (PDAC), no recurrent metastasis-specific mutation has been found, suggesting that epigenetic mechanisms, such as DNA methylation, are the major contributors of late-stage disease progression. Here, we performed the first whole-genome bisulfite sequencing (WGBS) on mouse and human PDAC organoid models to identify stage-specific and molecular subtype-specific DNA methylation
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Eukaryotic release factor 1 from Euplotes promotes frameshifting at premature stop codons in human cells iScience (IF 5.8) Pub Date : 2024-03-04 Bozhidar-Adrian Stefanov, Elvis Ajuh, Sarah Allen, Mariusz Nowacki
Human physiology is highly susceptible to frameshift mutations within coding regions, and many hereditary diseases and cancers are caused by such indels. Presently, therapeutic options to counteract them are limited and, in the case of direct genome editing, risky. Here, we show that release factor 1 (eRF1) from Euplotes, an aquatic protist known for frequent +1 frameshifts in its coding regions, can
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Mapping carbon reduction: A cross-continental study of alliance strategies iScience (IF 5.8) Pub Date : 2024-03-04 Congyue Zhou, Xingwei Xiang, Bifeng Zhu, Zhu Wang
Addressing the equitable distribution of global carbon emission rights is critical for sustainable development. Our research develops a detailed framework for a Global Carbon Reduction Alliance based on regional cooperation strategies, identifying key modes of intracontinental proximity and intercontinental distance collaboration. It emphasizes alliances formed among high carbon emission right countries