Abstract The oxidation of N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) using ammonium peroxydisulfate and the high redox potential laccase from Trametes hirsuta is studied in order to clarify the significant differences in the chemical and enzymatic polymerization reactions of aniline. As opposed to the laccase-catalyzed reaction, the chemical oxidation of TMPD proceeds in two stages. The radical

Abstract The 3D full-atom model of the whole-size CYP102A1 from Bacillus megaterium (cytochrome P450 BM3) has been constructed using molecular modeling methods. The structure model was constructed using crystal structures of the separate FAD-binding domain (PDB ID: 4DQK) and the complex of FMN-binding and monooxygenase domains (PDB ID: 1BVY). Modeling procedure included analysis of the domains’ surfaces

Abstract The results of a molecular dynamics study of the molecular mechanism of the experimentally observed specificity of the transaminase from Desulfohalobium retbaense (Dret) to the D-isomer of leucine are presented. A full-atom 3D model of Dret is constructed based on the data on the primary sequence and the information on the crystal structures of the related enzymes. The analysis of the trajectories

Abstract— Reporter vectors expressing of amino acid residues 16–96 and 16–85 from Neh2-domain of Nrf2 transcription factor fused to firefly luciferase have been constructed. Cell lines stably expressing the above reporter constructs as well as an earlier developed Neh2-luc fusion reporter have been generated using HeLa cells. Comparative analysis of all three reporter lines has been performed using

Abstract The channel proteins used in nanopore sequencing, their modifications, and auxiliary proteins regulating the process of nucleic acid translocation are considered. In the process of nanopore sequencing, the primary structure of the test DNA or RNA is decrypted by the translocation of a nucleic acid molecule through a nanopore intercalated into the membrane. A nanopore is a protein transmembrane

Abstract The rich chemical composition of essential oils (EOs) makes it possible to use them in various industries, especially in pharmacy, as pharmaceutical substances of plant origin as part of prophylactic/therapeutic products, as well as ready-made pharmaceuticals. Geranium EO was standardized according to the methods and quality indicators given in the general pharmacopeia monograph “Essential

Abstract Digitonin (monodesmoid saponin) forms complexes with cholesterol of cell membranes, causing the disintegration of the membrane structure, the formation of pores, and the release of intracellular components in the reaction medium. We use HEK293 cells transiently transfected with the pcDNALuc plasmid expressing firefly luciferase to study the kinetics of the initial stage of the interaction

Abstract For the experimental study of the role of the R65 residue in the stabilization of β-lactamases with the substitution of M182T, predicted by the analysis of the residue interaction networks (RINs), homogeneous preparations of the recombinant TEM-type β-lactamases with substitutions of R65L, M182A, and a combination of R65L and M182T mutations were obtained. The kinetic parameters of these enzymes

Abstract Complexes of a cationic pyridylphenylene dendrimer with solid and fluid anionic liposomes are prepared. Adsorption of the dendrimer on the surface of liposomes causes the neutralization of their surface charge and enlargement of the dendrimer–liposome aggregates. In interacting with fluid liposomes, the dendrimer does not induce the transition of negatively charged lipid molecules from the

Abstract The changes in the dispersion of inverse emulsions of oil and their stability during the formation/decomposition of natural gas hydrates are studied in the work. It is established that in the cycle of the formation/decomposition of natural gas hydrates, the aqueous phase coalesces in oil emulsions, containing more than 60 wt % of water, with the subsequent average diameter of droplets enlarging

Abstract The paper presents the results of the estimation of the KRASG12C ⋅ GDP ⋅ ARS-1620 complex dissociation constant. The ARS-1620 is a potential inhibitor of the cellular signal transduction of the KRAS enzyme with the oncogenic G12C mutation. The value of the dissociation constant estimated by molecular docking is 280 nM and 0.3 ± 0.1 nM within the molecular dynamics combined with the Hamiltonian

Abstract— The influence of nanostructure carbon additives (functionalized nanotubes, deaglomerated detonation nanodiamonds, nanocarbon) on the conductivity of the Aquivion perfluoropolymer membrane is studied. In the work the data are presented on the manufacturing of the composite materials and the investigation of their proton conductivity via the impedance spectroscopy method. It is found that the

Abstract New N-derivatives of daunorubicin are obtained using aromatic aldehydes by one-step reductive amination. The obtained compounds are found to exhibit a high cytotoxic activity.

Abstract The influence of nonionic surfactants on the bacteriolytic activity of lysozyme is studied on the model cells of Micrococcus luteus using Pluronics P123, L121, and F127, as well as Brij-35. These compounds form complexes with lysozyme through hydrogen-bonding interactions between the ethylene oxide fragments of surfactant and the amino acids residues of lysozyme surface. The bacteriolytic

Abstract The effect of the annealing temperature in the presence of mixtures of cadmium chloride and cadmium iodide of different compositions on the electrophysical properties of CdTe thin films is studied. This process, in which, as a rule, cadmium chloride is used, is called activation. It allows us to significantly improve the efficiency of solar cells based on CdTe. It is found that the temperature

Abstract Pipes with the defects of the welded joint (cavities, inclusions, brittle fracture) are studied, and their relationship with the characteristics of the feedstock is shown. Qualitative and quantitative indicators of the concentration of volatile substances of various fractions in the granules of polyethylene are obtained. The value of moisture absorption of carbon black concentrates used in

Abstract The speciation of 238, 239, 240Pu in the bottom sediments of two reservoirs of PA Mayak are investigated. The maximum content of plutonium in reservoir R-17 is found in the residual fraction. In reservoir R-4, plutonium is distributed equally in the residual fraction and the fraction bound to organic matter. The fact that plutonium exists in physico-chemically immobile fractions indicate its

Abstract A set of Pitzer interaction parameters is proposed to describe thermodynamic properties of the H2O–UO2(NO3)2–HNO3 solution within the temperature range of 15 to 50°C and ranges of concentrations of HNO3 and UO2(NO3)2 of 0 to 40 and 0 to 8 mol/kg, respectively. The experimental data on the vapor pressures of the volatile components of the system, degree of dissociation of nitric acid, and solid–liquid

Abstract The radiational chemical production of silver nanoparticles offers promising prospects for the synthesis of the antibacterial materials in the matrix of the nontoxic poly(vinyltriazole). Use of the optical spectroscopy for the electronic transmission microscopy shows that nanoparticles of the average size of 4 nm are formed in the radiated suspensions of poly(1-vinyl-1,2,4-triazole). The analysis

Abstract We demonstrate the possibility of the quantitative multielement analysis of a composite wear-resistant coating (tungsten carbide particles in chromium–nickel matrix) during its synthesis by coaxial laser cladding. The developed probe for the laser-induced breakdown spectrometry (LIBS) was used for the online analysis of the concentration of carbon, tungsten, and nickel in the process of applying

Abstract Ionic liquids (ILs) are successfully used in many fields of science, including the improvement of the performance of ion-selective sensors based on plasticized polymer matrices. However, the use of ionic liquids in optical sensors has not been sufficiently studied. In this paper, we study how the gradual replacement of the conventional plasticizer in the composition of the polymer optode matrix

Abstract This article studies the effects of solubility, disperse features (average diameter dav and charge (ζ‑potential)) of the particles of heavy-metal slightly soluble compounds exemplified by aluminum(III), iron(III), zinc(II), and lead(II) hydrates related to the efficiency of their removal from aqueous solutions in the composition by electroflotation and filtration within the pH range 5–12.

Abstract The recombinant rPichia/lip lipase was immobilized by adsorbing the enzyme on aggregated carbon nanotubes (CNTs), unmodified, or doped with nitrogen (N-CNTs). Heterogeneous biocatalysts prepared in this way were investigated in the reaction of the low-temperature synthesis of esters proceeding in organic solvents at room temperature. We studied the effect of the texture and morphology of the

Abstract A rapid method for quantitation of lytic polysaccharide monooxygenase (LPMO) in multienzyme cocktails produced by mutant strains of filamentous fungi is developed. In this method, 2,6-dimethoxyphenol and hydrogen peroxide are used as cosubstrates in a nonspecific LPMO-catalyzed reaction leading to the formation of a colored product. With this method, we determine the LPMO content in the multienzyme

Abstract The results of qualitative and quantitative determination of polyphenolic compounds, in particular flavon glycosides and flavon aglycones, in alcohol–water extracts from Canadian goldenrod herbs, flowers, and leaves and alcohol–water extracts from dwarf goldenrod flowers and leaves by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) are presented

Abstract The new plant raw material from leaves and fruits of Malus sylvestris is analyzed. It is shown that the content of substances extracted with acetonitrile amounted to 4.7–5.4% for leaves and 2.3–2.8% for fruits. The obtained extracts were used to identify substances, the presence of which is specific for the plant’s raw material and can be used in the future to identify raw material in crushed

Abstract The effect of solid dispersions (SDs) on the solubility of furazolidone is studied. Furazolidone and its SDs with polyvinylpyrrolidone (PVP), PVP-10 000, PVP-12 600, and PVP-24 000 are examined. When using SDs, the solubility and dissolution rate of the nitrofuran derivative is increased. The solubility of furazolidone from SD is increased by a factor of 1.1 to 1.6. The dissolution rate of

Abstract The possibility of the use of a collision and reaction cell is shown and the optimum parameters for its adjustment are selected for the determination of Si, B, Ca, Mg, Ba, and Zr in a complex-alloyed nickel alloy via inductively coupled plasma mass spectrometry (ICP-MS). The procedure of sample dissolution and its preparation for the analysis is presented. The correctness of the obtained results