Abstract The spin-probe method is applied to estimate the rate and degree of sorption of water vapor by gelatin-based polymers. The rate and the sorption degree of gelatins are shown to be affected by the extent that they are crosslinked. Cross-linked gelatin swells in water vapor at 300 K for a time period that is thrice as long as the time for which native gelatin swells in the same conditions.

Abstract Maghemite nanoparticles of 40 to 150 nm are obtained by the thermal decomposition of preliminarily cryochemically modified iron acetylacetonate. The composition and structure of the obtained particles and cryomodified precursor salt are determined by the X-ray diffraction (XRD) analysis, thermoanalytical methods (thermogravimetry, differential scanning calorimetry), infrared spectroscopy,

Abstract The structural-group composition and the properties of organosulfur compounds of oil fraction of Bavly petroleum are studied. We show that organosulfur compounds are predominantly (64.7%) made up of sulfides. The mass spectroscopic data indicate that the sulfides of the oil fraction contain mainly the saturated compounds in the absence of aromatic sulfides. The organosulfur compounds of the

Abstract Comparative quantitative evaluation of estrogen receptors β (ERβ) expression is performed by the immunofluorescence method using flow cytometry in the ovarian cancer cell lines COLO-704, OVCAR-3, EFO-21, and SK-OV-3, as well as the breast cancer cell line MCF-7. A high level of ERβ expression (88–91%) is revealed in all the cell cultures investigated, and significant differences were observed

Abstract A novel chi19 gene encoding the chitinase, belonging to the glycosyl hydrolase family 19 from the predatory plant Drosera capensis is cloned and expressed in the E. coli bacterial system. The amino acid sequence of the translated enzyme consists of 325 a.a. divided into four functional parts: the N-terminal signal sequence and of the catalytic and chitin-binding domains that were linked by

Abstract We proposed a method for producing magnetic nanocomposite adsorbents, based on the adsorption of Fe3O4 magnetic nanoparticles (MNPs) on hydrophobized silicas with different structural characteristics. The magnetic properties, stability, and structural characteristics of the obtained magnetic adsorbents are studied. The effect of the incorporation of Fe3O4 nanoparticles into the adsorbent matrix

Abstract We study the effect of surfactants having a different charge and length of the hydrocarbon radical on the intensity and kinetic laws of the model chemiluminescent reaction of a hydrophobic analogue of luminol, N-octyl luminol. The effect of the solution pH and the charge, structure, and concentration of surfactants on the intensity of the chemiluminescence is assessed, and the kinetic laws

Abstract The conditions for the quantitative determination of acetylsalicylic acid (ASA) in Rhodococcus culture fluids via the reverse-phase high-performance liquid chromatography are developed. The rate of biodegradation process and the half-life of acetylsalicylic acid as a pharmaceutical substance and tablets are found.

Abstract Optimizing the composition of an agroinfiltration solution is one way to boost the efficiency of the transient expression of the target protein in plants by using Agrobacterium tumefaciens. We use the design of the experiment to investigate the effects that some chemical additives introduced into the agroinfiltration medium have on the yield of the target immunocytokine. It is shown that acetosyringone

Abstract The paper presents the results of a study of the antioxidant and antibacterial activity of goldenrod grass. Using the method of spectrophotometry and reaction with the radical 1,1-diphenyl-2-picrylhydrazyl, the antioxidant activity of the alcohol-water extraction of Canadian goldenrod grass is determined. An aqueous-water extraction from the grass of the Canadian goldenrod also exhibits antibacterial

Abstract We study the factors affecting the size of two-layer polyionic bienzyme SOD1/catalase conjugates: the nature and concentration of the buffer solution, the pH maintained during particle synthesis, and the initial molar ratio of the enzymes. The optimal conditions for obtaining conjugates of the minimum size are determined. The conjugates obtained under these conditions demonstrate the high

Abstract Cryochemical modification methods are used to obtain hybrid composites of the antibacterial drug gentamicin sulfate, as well as copper and iron nanoparticles, which are promising precursors of the targeted drug delivery systems. The resulting systems are antibiotic nanoparticles of 50 to 300 nm, including copper and iron nanoparticles of 1 to 20 nm (according to the results of physicochemical

Abstract We study the effect of the nature of the substituent in the β-cyclodextrin derivative on the physicochemical properties of the antibacterial drug ciprofloxacin (CF). CF can form guest–host inclusion complexes with β-cyclodextrin derivatives, characterized by dissociation constants (Kdis) in the range of 10–3 to 10–4 M. A ligand with a small polar uncharged substituent (2-hydroxypropyl) form

Abstract We optimize the procedure for synthesizing gold nanoshells for their use as a sensitive label in an immunochromatographic assay (ICA). An immunochromatographic test system is developed to visually determine the concentration of an early cardio marker, a heart-type fatty acid-binding protein (H-FABP). Visual detection yields the detection limit of the procedure with gold nanoshells to be more

Abstract Magnetite-gold hybrid magnetic nanoparticles of the dumbbell type were used to immobilize two proteins: α-chymotrypsin and the Bowman–Birk inhibitor. It is shown that under the influence of a low-frequency magnetic field (50 Hz), the activity of chymotrypsin bound in a complex with an inhibitor is drastically reduced depending on the magnetic field induction. We hypothesize that this effect

Abstract The cas9 gene of the Streptococcus pyogenes bacterium, which encodes Cas9 nuclease of the class 2 CRISPR/Cas system, is expressed in the filamentous fungus Penicillium verruculosum under the control of the constitutive autologous promoter of the glyceraldehyde-3-phosphate dehydrogenase gpdA gene, which is cloned and sequenced for the first time in the present work. The functionality of the

Abstract Magnetic anionic liposomes (MALip) conjugated with magnetite magnetic nanoparticles (MNPs) are developed for the controlled release of a protease inhibitor (BBI) under exposure to a low-frequency nonheating magnetic field (LF AMF). It is shown that an increase of up to 35% of the protein release rate occurred when the MALip are exposed to the LF AMF (frequency 110 Hz, intensity 75–150 kA/m)

Abstract We present a comprehensive study of the receptor–ligand interaction by spectral methods on the example of Concanavalin A lectin and 5 kDa chitosan modified with D-mannose. An improved method for the modification of chitosan with D-mannose and purification of these conjugates is developed. A novel approach to calculate the constants of the Concavalin A binding with the mannose-containing ligand

Abstract NAD(P)+-dependent formate dehydrogenase (FDH, EC 1.2.1.2.) is actively used in processes of chiral synthesis by oxidoreductases with systems of reduced cofactor regeneration. The efficient use of FDH in such systems requires simple and fast enzyme purification. Metal-chelate affinity chromatography is widely used for such purposes. The method requires the presence of at least six His residues

AbstractThe oxidation of N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) using ammonium peroxydisulfate and the high redox potential laccase from Trametes hirsuta is studied in order to clarify the significant differences in the chemical and enzymatic polymerization reactions of aniline. As opposed to the laccase-catalyzed reaction, the chemical oxidation of TMPD proceeds in two stages. The radical

AbstractThe 3D full-atom model of the whole-size CYP102A1 from Bacillus megaterium (cytochrome P450 BM3) has been constructed using molecular modeling methods. The structure model was constructed using crystal structures of the separate FAD-binding domain (PDB ID: 4DQK) and the complex of FMN-binding and monooxygenase domains (PDB ID: 1BVY). Modeling procedure included analysis of the domains’ surfaces

AbstractThe results of a molecular dynamics study of the molecular mechanism of the experimentally observed specificity of the transaminase from Desulfohalobium retbaense (Dret) to the D-isomer of leucine are presented. A full-atom 3D model of Dret is constructed based on the data on the primary sequence and the information on the crystal structures of the related enzymes. The analysis of the trajectories

Abstract—Reporter vectors expressing of amino acid residues 16–96 and 16–85 from Neh2-domain of Nrf2 transcription factor fused to firefly luciferase have been constructed. Cell lines stably expressing the above reporter constructs as well as an earlier developed Neh2-luc fusion reporter have been generated using HeLa cells. Comparative analysis of all three reporter lines has been performed using

AbstractThe channel proteins used in nanopore sequencing, their modifications, and auxiliary proteins regulating the process of nucleic acid translocation are considered. In the process of nanopore sequencing, the primary structure of the test DNA or RNA is decrypted by the translocation of a nucleic acid molecule through a nanopore intercalated into the membrane. A nanopore is a protein transmembrane

AbstractThe rich chemical composition of essential oils (EOs) makes it possible to use them in various industries, especially in pharmacy, as pharmaceutical substances of plant origin as part of prophylactic/therapeutic products, as well as ready-made pharmaceuticals. Geranium EO was standardized according to the methods and quality indicators given in the general pharmacopeia monograph “Essential

AbstractDigitonin (monodesmoid saponin) forms complexes with cholesterol of cell membranes, causing the disintegration of the membrane structure, the formation of pores, and the release of intracellular components in the reaction medium. We use HEK293 cells transiently transfected with the pcDNALuc plasmid expressing firefly luciferase to study the kinetics of the initial stage of the interaction of

AbstractFor the experimental study of the role of the R65 residue in the stabilization of β-lactamases with the substitution of M182T, predicted by the analysis of the residue interaction networks (RINs), homogeneous preparations of the recombinant TEM-type β-lactamases with substitutions of R65L, M182A, and a combination of R65L and M182T mutations were obtained. The kinetic parameters of these enzymes

AbstractComplexes of a cationic pyridylphenylene dendrimer with solid and fluid anionic liposomes are prepared. Adsorption of the dendrimer on the surface of liposomes causes the neutralization of their surface charge and enlargement of the dendrimer–liposome aggregates. In interacting with fluid liposomes, the dendrimer does not induce the transition of negatively charged lipid molecules from the

AbstractThe changes in the dispersion of inverse emulsions of oil and their stability during the formation/decomposition of natural gas hydrates are studied in the work. It is established that in the cycle of the formation/decomposition of natural gas hydrates, the aqueous phase coalesces in oil emulsions, containing more than 60 wt % of water, with the subsequent average diameter of droplets enlarging

AbstractThe paper presents the results of the estimation of the KRASG12C ⋅ GDP ⋅ ARS-1620 complex dissociation constant. The ARS-1620 is a potential inhibitor of the cellular signal transduction of the KRAS enzyme with the oncogenic G12C mutation. The value of the dissociation constant estimated by molecular docking is 280 nM and 0.3 ± 0.1 nM within the molecular dynamics combined with the Hamiltonian

Abstract—The influence of nanostructure carbon additives (functionalized nanotubes, deaglomerated detonation nanodiamonds, nanocarbon) on the conductivity of the Aquivion perfluoropolymer membrane is studied. In the work the data are presented on the manufacturing of the composite materials and the investigation of their proton conductivity via the impedance spectroscopy method. It is found that the

AbstractNew N-derivatives of daunorubicin are obtained using aromatic aldehydes by one-step reductive amination. The obtained compounds are found to exhibit a high cytotoxic activity.

AbstractThe influence of nonionic surfactants on the bacteriolytic activity of lysozyme is studied on the model cells of Micrococcus luteus using Pluronics P123, L121, and F127, as well as Brij-35. These compounds form complexes with lysozyme through hydrogen-bonding interactions between the ethylene oxide fragments of surfactant and the amino acids residues of lysozyme surface. The bacteriolytic activity

AbstractThe effect of the annealing temperature in the presence of mixtures of cadmium chloride and cadmium iodide of different compositions on the electrophysical properties of CdTe thin films is studied. This process, in which, as a rule, cadmium chloride is used, is called activation. It allows us to significantly improve the efficiency of solar cells based on CdTe. It is found that the temperature

AbstractPipes with the defects of the welded joint (cavities, inclusions, brittle fracture) are studied, and their relationship with the characteristics of the feedstock is shown. Qualitative and quantitative indicators of the concentration of volatile substances of various fractions in the granules of polyethylene are obtained. The value of moisture absorption of carbon black concentrates used in

AbstractThe speciation of 238, 239, 240Pu in the bottom sediments of two reservoirs of PA Mayak are investigated. The maximum content of plutonium in reservoir R-17 is found in the residual fraction. In reservoir R-4, plutonium is distributed equally in the residual fraction and the fraction bound to organic matter. The fact that plutonium exists in physico-chemically immobile fractions indicate its

AbstractA set of Pitzer interaction parameters is proposed to describe thermodynamic properties of the H2O–UO2(NO3)2–HNO3 solution within the temperature range of 15 to 50°C and ranges of concentrations of HNO3 and UO2(NO3)2 of 0 to 40 and 0 to 8 mol/kg, respectively. The experimental data on the vapor pressures of the volatile components of the system, degree of dissociation of nitric acid, and solid–liquid

AbstractThe radiational chemical production of silver nanoparticles offers promising prospects for the synthesis of the antibacterial materials in the matrix of the nontoxic poly(vinyltriazole). Use of the optical spectroscopy for the electronic transmission microscopy shows that nanoparticles of the average size of 4 nm are formed in the radiated suspensions of poly(1-vinyl-1,2,4-triazole). The analysis

AbstractWe demonstrate the possibility of the quantitative multielement analysis of a composite wear-resistant coating (tungsten carbide particles in chromium–nickel matrix) during its synthesis by coaxial laser cladding. The developed probe for the laser-induced breakdown spectrometry (LIBS) was used for the online analysis of the concentration of carbon, tungsten, and nickel in the process of applying

AbstractIonic liquids (ILs) are successfully used in many fields of science, including the improvement of the performance of ion-selective sensors based on plasticized polymer matrices. However, the use of ionic liquids in optical sensors has not been sufficiently studied. In this paper, we study how the gradual replacement of the conventional plasticizer in the composition of the polymer optode matrix

AbstractThis article studies the effects of solubility, disperse features (average diameter dav and charge (ζ‑potential)) of the particles of heavy-metal slightly soluble compounds exemplified by aluminum(III), iron(III), zinc(II), and lead(II) hydrates related to the efficiency of their removal from aqueous solutions in the composition by electroflotation and filtration within the pH range 5–12. The

AbstractThe recombinant rPichia/lip lipase was immobilized by adsorbing the enzyme on aggregated carbon nanotubes (CNTs), unmodified, or doped with nitrogen (N-CNTs). Heterogeneous biocatalysts prepared in this way were investigated in the reaction of the low-temperature synthesis of esters proceeding in organic solvents at room temperature. We studied the effect of the texture and morphology of the

AbstractA rapid method for quantitation of lytic polysaccharide monooxygenase (LPMO) in multienzyme cocktails produced by mutant strains of filamentous fungi is developed. In this method, 2,6-dimethoxyphenol and hydrogen peroxide are used as cosubstrates in a nonspecific LPMO-catalyzed reaction leading to the formation of a colored product. With this method, we determine the LPMO content in the multienzyme

AbstractAn effective, affordable, and rapid method for the analysis of drugs based on chloramphenicol is developed using multisensory digital colorimetry. The use of a two-dimensional code for the identification and quantification of chloramphenicol with the minimum level of informational noise is proposed. The principal component analysis is used to increase the reliability of the results of the determination

AbstractBased on the experimental studies of the dissolution of Cr2O3 and CuO in an acidic environment, as well as investigation of the effect of acids, pH, and concentration of anions on the rate of dissolution of the oxides, the nature of the limiting stage is determined which consists in the formation of surface compounds (depending on the acidity of the environment) of the \({\text{MeOH}}_{s}^{{x

AbstractThe distinctive features of firefly luciferase bioluminescence are complex changes in the shape of the spectra and λmax of bioluminescence with varying pH, temperature, and enzyme structure. An analysis of the published data and the authors’ own results leads to the conclusion that the keto–enol tautomerism of the oxyluciferin molecule explains the observed complex spectral changes most reliably

AbstractA study of kerogen-containing oil shale rock using IR spectroscopy and pyrolysis methods (the Rock-Eval method) is carried out. Using the Domanic sedimentary rock samples, it is shown that studies by Fourier-transform IR spectroscopy in combination with the pyrolytic method provide an opportunity to obtain information on the group composition of organic matter and to evaluate the oil-generation

AbstractThe extraction mechanism of molybdenum(VI) ions from model solutions with iron-containing metalworking wastes is studied. The experimental X-ray phase analysis and energy dispersive microanalysis data indicate that iron(III) molybdate appears in the first chemisorption stages with the subsequent formation of layers enriched in molybdenum(VI) oxide. The kinetics of the chemisorption of molybdenum(VI)

AbstractIn order to control the maximum levels of As, Cr, Pb, Cd, and Sn in meat products, it is proposed to transfer them to a suspension of 140 mg/mL and use an atomic absorption spectrometer with a graphite furnace, equipped with a probe attachment for a two-stage atomization of the suspension. The working linear range covers 0.2–9 ultimate allowable concentrations. The error is smaller by factors

AbstractThe results of qualitative and quantitative determination of polyphenolic compounds, in particular flavon glycosides and flavon aglycones, in alcohol–water extracts from Canadian goldenrod herbs, flowers, and leaves and alcohol–water extracts from dwarf goldenrod flowers and leaves by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) are presented

AbstractThe new plant raw material from leaves and fruits of Malus sylvestris is analyzed. It is shown that the content of substances extracted with acetonitrile amounted to 4.7–5.4% for leaves and 2.3–2.8% for fruits. The obtained extracts were used to identify substances, the presence of which is specific for the plant’s raw material and can be used in the future to identify raw material in crushed

AbstractThe effect of solid dispersions (SDs) on the solubility of furazolidone is studied. Furazolidone and its SDs with polyvinylpyrrolidone (PVP), PVP-10 000, PVP-12 600, and PVP-24 000 are examined. When using SDs, the solubility and dissolution rate of the nitrofuran derivative is increased. The solubility of furazolidone from SD is increased by a factor of 1.1 to 1.6. The dissolution rate of

AbstractThe possibility of the use of a collision and reaction cell is shown and the optimum parameters for its adjustment are selected for the determination of Si, B, Ca, Mg, Ba, and Zr in a complex-alloyed nickel alloy via inductively coupled plasma mass spectrometry (ICP-MS). The procedure of sample dissolution and its preparation for the analysis is presented. The correctness of the obtained results

Abstract—The definitions of the critical contact and critical energy coordination number (CECN) of a molecular crystal are introduced. Some characteristics of the critical contact in the diagram of intermolecular interaction energies are discussed. The molecular coordination number (MCN) and critical coordination number (CCN) in a series of crystalline structures of homomolecular monosystem aliphatic

AbstractComposite mixed-matrix membranes for gas separation containing copolyetherimide Siltem® as the polymer matrix and metal-organic framework MIL-101 (10 wt %) as the active filler, were obtained using dry and wet-dry formation techniques. It has been found that the polymer concentration in the initial solution does not significantly affect the CO2 and CH4 permeability of the film membranes obtained

Abstract—2G HTSC tapes YBCO/LZO/Ni-5W and YBCO/Y2O3/LZO/Ni-5W with the minimum number of layers are obtained. Heteroepitaxial structures (layers La2Zr2O7, Y2O3 and YBa2Cu3O7) are formed by chemical deposition from a solution and chemical vapor deposition. Their textural quality is confirmed by XRD. The influence of the layers on each other is shown. Many factors for improving the quality of the 2G

Abstract—A catalyst based on ionic liquid consisting of a pyridinium cation having Brønsted acidity and a molybdenum anion (1-butyl-3-carboxypyridinium molybdate) is obtained. Its catalytic activity in the oxidation of sulfur-containing compounds is studied. The influence of the oxidation’s duration, temperature, and the quantity of the oxidation agent and catalyst on the conversion of dibenzothiophene

Abstract—A new method of double immunofluorescence analysis by flow cytometry is developed to evaluate the quantitative expression of the mesenchymal marker vimentin in epithelium cells of a solid tumor, which in turn is a mixture of different histogenesis cells. The method has a number of evident advantages compared to the clinically used immunohistochemistry technique for marker investigation: the

Abstract—Molecular heterogeneity in seven human metastatic melanoma cells lines is revealed using a double immunofluorescent assay by flow cytometry. It is evaluated that quite a small population of cells (up to 10%) expressed only vimentin, the main marker of melanoma. Only epithelial cytokeratins expression is revealed in a significantly greater number of cells (from 25 to 50%). The dominant molecular

Abstract—The paper describes the production of a number of lipoamino acid derivatives based on L-serine, L-ornithine, and L-lysine with potential antibacterial activity. The designed synthesis schemes are distinguished by the simplicity and versatility of the approach used. This allows us to use them to obtain a series of samples in preparative quantities required to perform the subsequent physico-chemical