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Selective visual detection of multiplex PCR amplicon using magnetic microbeads Biosens. Bioelectron. X Pub Date : 2024-03-14 Michihiko Nakano, Masafumi Inaba, Junya Suehiro
Nucleic acid amplification tests (NATs), such as genetic tests using polymerase chain reaction (PCR), are sensitive methods for detecting pathogens and food contamination. The rapid, easy, and inexpensive detection of amplicons, DNA, or RNA is key to realizing on-site NATs. We have previously developed a novel amplicon detection method using magnetic microbeads based on the hydrophobicity of DNA in
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A study on sensitivity, reproducibility, and stability of sourness and saltiness taste sensors using a mixture of lipid membranes Biosens. Bioelectron. X Pub Date : 2024-03-13 Rita Prasetyowati, Rizky Aflaha, Hanif Khoirudin, Moch Rifqi Tamara, Roto Roto, Pekik Nurwantoro, Kuwat Triyana
Our study successfully fabricated sourness and saltiness sensors and investigated the response toward acetic acid and sodium chloride as the sourness and saltiness sample solutions, respectively. The sensors were made using a lipid membrane with a mixture of two materials. The fabricated sensors can detect the concentration of a small amount of 0.03 mM for the sourness sensor and 0.316 mM for the saltiness
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Purpald-functionalized biosensor for simultaneous electrochemical detection of ascorbic acid, uric acid, L-cysteine and lipoic acid Biosens. Bioelectron. X Pub Date : 2024-03-08 Pandiaraja Varatharajan, Muniyandi Maruthupandi, Vinoth Kumar Ponnusamy, Nagamalai Vasimalai
The polymer purpald (4-amino-3-hydrazino-5-mercapto-1,2,4-triazole) film on glassy carbon electrode (p-Purpald@GCE) was fabricated using purpald monomer by electropolymerization method, and the modified electrode was applied as a probe for the simultaneous sensing of ascorbic acid (AA), uric acid (UA), L-cysteine (L-Cys) and lipoic acid (LA). The modified p-Purpald@GCE was well characterized by Scanning
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Gold nanoparticle–based lateral-flow immunochromatographic biosensing assays for the diagnosis of infections Biosens. Bioelectron. X Pub Date : 2024-03-06 Olga I. Guliy, Lev A. Dykman
Infections cause concern for mankind, and sometimes their diagnosis may take much time. Owing to its high sensitivity, rapidity, and specificity, the immunochromatographic assay (ICA) is used widely to diagnose infections. The use of the ICA for this purpose makes it possible to detect dangerous diseases early, prevent their progress, strongly reduce the treatment cost and mortality, and increase life
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Non-enzymatic electrochemical sensing platform based on metal oxide-loaded biopolymer for voltammetric measurement of hepatoprotective metadoxine drug in pharmaceutical formulation and human blood serum Biosens. Bioelectron. X Pub Date : 2024-02-17 Manish S. Sengar, Priya Kumari, Neha Sengar, Soami P. Satsangee, Rajeev Jain
This study focuses on the first-ever high-sensitivity, low-cost, and quick response electrochemical estimation of metadoxine (MTD) in pharmaceuticals and human blood serum using a non-enzymatic nanocomposite modified glassy carbon electrode (CuO/CH/GCE) loaded with chitosan. The electroactive surface of GCE was produced by drop-casting a suspension of CuO/CH nanocomposite in N,N-dimethylformamide (DMF)
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Reduced amplification by phi29 DNA polymerase in the presence of unbound oligos during reaction in RCA Biosens. Bioelectron. X Pub Date : 2024-02-15 Darío Sánchez Martín, Tingting Li, Marie Wrande, Linus Sandegren, Bo Tian, Maria Strømme, Teresa Zardán Gómez de la Torre
Synthetic single-stranded oligonucleotides play crucial roles in DNA amplification reactions for various applications, such as serving as primers, enabling magnetic separation, and generating dsDNA for subsequent digestion. Typically, these oligos are added in excess to ensure rapid binding to their intended targets. However, while performing rolling circle amplification (RCA) using phi29 DNA polymerase
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Robust and flexible electrochemical lactate sensors for sweat analysis based on nanozyme-enhanced electrode Biosens. Bioelectron. X Pub Date : 2024-02-15 Pei Li, Pramod K. Kalambate, Kenneth D. Harris, Abebaw B. Jemere, Xiaowu (Shirley) Tang
In this work, nickel oxide (NiO) nanostructures deposited by glancing angle deposition (GLAD) are fabricated to achieve highly specific catalytic electrooxidation of lactate, replacing the natural enzyme lactate oxidase for electrochemical detection of lactate in sweat. GLAD NiO electrodes exhibit high sensitivity (412 μA mM cm), wide linear detection range (1–45 mM), low detection limit (3 μM), and
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Vibrio vulnificus marine pathogen detection with thin-film impedance biosensors Biosens. Bioelectron. X Pub Date : 2024-02-11 Arnau Pérez Roig, Bergoi Ibarlucea, Carmen Amaro, Gianaurelio Cuniberti
(Vv) is a marine pathogen that can cause rapid death by septicemia (vibriosis) in humans and several fish species. This pathogen is considered a biomarker of climate change, as both its presence and vibriosis incidence in coastal environments are increasing because of global warming. Currently, gold-standard methods for Vv detection are all PCR-based, requiring expensive equipment and skilled personnel
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Portable optical biosensor for point-of-care monitoring of salivary glucose using a paper-based microfluidic strip Biosens. Bioelectron. X Pub Date : 2024-02-10 Shweta Panwar, Paulami Sarkar, D. Syed Kasim, Raksha Anand, Akanksha Priya, Shyam Prakash, Sandeep Kumar Jha
The manuscript describes a technique for fabrication and validation of a standalone handheld optical biosensor designed for non-invasive monitoring of glucose through saliva. In this cost-effective process, a 3D-printed glucose test strip was filled with sieving paste comprising of cellulose, polyethylene glycol (PEG), polyvinyl alcohol (PVA) and glycerol, onto which, glucose oxidase-peroxidase (GOD-POD)
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Non-enzymatic electrochemical detection of methylglyoxal in saliva using a polyaniline/nickel oxide nanohybrid biosensor: A noninvasive approach for diabetes diagnosis Biosens. Bioelectron. X Pub Date : 2024-01-27 Subramanian Vasanth, Humayun Amir, Nagomony Ponpandian, Chinnuswamy Viswanathan
Methylglyoxal (MG) is a prominent biomarker for diabetic syndromes and ageing disorders. In the present study, a non-enzymatic electrochemical detection of MG in human saliva at low levels was achieved via polyaniline/nickel oxide nanohybrid modified graphite sheet (PANI/NiO/GS) electrode. The physical characteristics and structure of the PANI/NiO nanohybrid showed the presence of NiO flakes embedded
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Modern technology advances of Pseudomonas aeruginosa based biosensor approach Biosens. Bioelectron. X Pub Date : 2024-01-22 Dhanendiren Narayanasamy, Safura Taufik, Ahmad Farid Mohd Azmi, Siti Aminah Mohd Nor, Jahwarhar Izuan Abdul Rashid
The threat posed by microbial infections to human health remains very significant, particularly in considering the increasing mechanisms of antibiotic resistance. Pseudomonas aeruginosa is known for its antibiotic resistance and is linked to serious conditions like ventilator-associated pneumonia and cystic fibrosis. Rapid infection elimination relies on identifying harmful bacteria and preventing
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Electrochemical framework for dynamic tracking of Soil Organic Matter Biosens. Bioelectron. X Pub Date : 2024-01-19 Vikram Narayanan Dhamu, Anirban Paul, Sriram Muthukumar, Shalini Prasad
Soil Health parameters serve as excellent surrogate measures towards assessing environmental quality and understanding effects of climate change mitigation via carbon sequestration. Soil Organic Matter (SOM) is a parameter that is synonymous to soil health and understanding SOM is a key metric to building and influencing good soil and agronomic practices by impacting soil aggregation and water withholding
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Reduced graphene oxide-gadolinium oxide-functionalized paper based immunosensor for electrochemical detection of gentamicin Biosens. Bioelectron. X Pub Date : 2024-01-19 Jayendra Kumar Himanshu, G.B.V.S. Lakshmi, Akhilesh Kumar Singh, Pratima R. Solanki
Excessive consumption of antibiotics like gentamicin (GEN) can lead to hostile effects as antibiotic resistance. Therefore, the detection is important for which, reduced graphene oxide-Gadolinium oxide nanocomposite (rGO@Gd2O3 NC) was composed through co-precipitation method for the detection of GEN. The structural, morphological and functional group characterizations were done using XRD, FT-IR, SEM
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Collaborative biofluid analysis based multi-channel integrated wearable detection system for the monitoring of wound infection Biosens. Bioelectron. X Pub Date : 2024-01-18 Zetao Chen, Yifan Dong, Jie Fu, Yongchang Bai, Qiya Gao, Ziyue Qin, Jiawang Wang, Shuang Li
The infection monitoring of chronic wounds can effectively improve the quality of wound care. However, the widely used single variable intermittent monitoring of wound provides little available information, which leads to inaccurate diagnosis and untimely warnings. In this study, a collaborative biofluid analysis based multi-channel integrated wearable detection system was constructed for the continuous
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Development of chrono-spectral gold nanoparticle growth based plasmonic biosensor platform Biosens. Bioelectron. X Pub Date : 2024-01-11 A. Baran Sözmen, Beste Elveren, Duygu Erdogan, Bahadır Mezgil, Yalın Bastanlar, U. Hakan Yildiz, Ahu Arslan Yildiz
Plasmonic sensor platforms are designed for rapid, label-free, and real-time detection and they excel as the next generation biosensors. However, current methods such as Surface Plasmon Resonance require expertise and well-equipped laboratory facilities. Simpler methods such as Localized Surface Plasmon Resonance (LSPR) overcome those limitations, though they lack sensitivity. Hence, sensitivity enhancement
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Longitudinal monitoring of IL-6 and CRP in inflammatory bowel disease using IBD-AWARE Biosens. Bioelectron. X Pub Date : 2024-01-06 Robert P. Hirten, Kai-Chun Lin, Jessica Whang, Sarah Shahub, Nathan K.M. Churcher, Drew Helmus, Sriram Muthukumar, Bruce Sands, Shalini Prasad
There are limitations to monitoring modalities for chronic inflammatory conditions, including inflammatory bowel disease (IBD). Wearable devices are scalable mobile health technology that present an opportunity to monitor markers that have been linked to worsening, chronic inflammatory conditions and enable remote monitoring. In this research article, we evaluate and demonstrate a proof-of-concept
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Carbon-stabilized porous silicon biosensor for the ultrasensitive label-free electrochemical detection of bacterial RNA gene fragments Biosens. Bioelectron. X Pub Date : 2024-01-06 Grace Pei Chin, Keying Guo, Roshan Vasani, Nicolas H. Voelcker, Beatriz Prieto-Simón
Herein, we report a carbon-stabilized porous silicon (pSi)-based electrochemical biosensing platform for the label- and amplification-free detection of bacterial 16S rRNA gene fragments that facilitates pan-bacterial detection. The sensing approach combines thermally carbonized pSi (THCpSi) structures as novel porous electrochemical transducers, and a highly sensitive sensing mechanism based on partial
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DNA origami-enhanced binding of aptamers to Staphylococcus aureus cells Biosens. Bioelectron. X Pub Date : 2024-01-05 Yasaman Ahmadi, Trishant R. Umrekar, Natalie Mutter, Morgan Beeby, Ivan Barišić
The combination of DNA origami nanostructures and aptamers provides a powerful technology for diagnostic assays. Here, we functionalized a DNA origami nanostructure with a Protein-A binding aptamer to target Staphylococcus aureus bacterial cells. Using an enzyme-linked oligonucleotide assay (ELONA), we semi-quantitatively analyzed and compared the interaction of the aptamer and aptamer-modified DNA
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A novel modular opto-biomechatronics bioreactor for simultaneous isotropic mechanical stretch application and fluorescence microscopy under cell and tissue culture conditions Biosens. Bioelectron. X Pub Date : 2024-01-05 Anna-Lena Merten, Ulrike Schöler, Christian Lesko, Lucas Kreiß, Dominik Schneidereit, Fabian Linsenmeier, Axel Stolz, Sebastian Rappl, Mohamed Ali, Tim Potié, Adel Ahmed, Jordi Morales-Dalmau, Jan Saam, Sebastian Schürmann, Oliver Friedrich
Mechanical stresses are an environmental challenge virtually all tissues in the body are exposed to and thus, are of fundamental interest to study cell reactions in mechanobiology. Yet, unlike acute short-term mechanical cell stimulations, long-term or cyclic mechano-stimulation as experienced in the body is difficult to reproduce. Bioreactors are designed to control cell culture conditions, but still
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An integrated digital microfluidic electrochemical impedimetric lipopolysaccharide sensor based on toll-like receptor-4 protein Biosens. Bioelectron. X Pub Date : 2024-01-02 Richard P.S. de Campos, Dipesh Aggarwal, Nora W.C. Chan, Abebaw B. Jemere
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Multi-layer SPR biosensor for in-Situ Amplified monitoring of the SARS-CoV-2 omicron (B.1.1.529) variant Biosens. Bioelectron. X Pub Date : 2024-01-01 Tarik Bin Abdul Akib, Md Masud Rana, Ibrahim M. Mehedi
This article represents an analysis of the performance of multi-layer surface plasmon resonance (SPR) biosensors in detecting the transferable human SARS-CoV-2 Omicron (B.1.1.529) variant. The proposed multi-layer SPR biosensor performance is enhanced by integrating fine-tuning prisms, plasmonic metals, and two-dimensional (2D) transition metal dichalcogenides (TMDs) materials. To evaluate the performance
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Review on current race for Covid-19 diagnosis Biosens. Bioelectron. X Pub Date : 2023-12-17 Kanchan Karki, Sonali Priyadarshini, Piyush Kumar, Sanjay Kumar, R.S. Kundu, Krishna Pal Singh, Aryan Singh Lather, Kanika Poonia, Anuj Nehra
The enduringsevere acute respiratory syndrome corona virus 2 (SARS-CoV-2) global health has led to a huge market to design affordable point-of-care (PoC) examination tools and skilled laboratory procedures for its recognition. Most of the available tests for early discovery of SARS-CoV-2 nucleic acids are built on the reverse transcription polymerase chain reaction (RT-PCR); however, isothermal nucleic
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3D-printed cassette integrated with paper-based aptasensor for the construction of next-generation sensing tool to detect dengue virus towards plaspertronix-commercialization Biosens. Bioelectron. X Pub Date : 2023-12-21 Mohd. Rahil Hasan, Pradakshina Sharma, Saumitra Singh, Annu Mishra, Zaira Azmi, Jagriti Narang
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Recent developments and trends of biosensors based on carbon nanotubes for biomedical diagnosis applications: A review Biosens. Bioelectron. X Pub Date : 2023-12-19 Yan Zhang, Junfeng Guo, Zhaoxiang Tang, Chuyue Tang, Yiang Li, Xu Tao, Binghua Zhou, Wan Chen, Lin Guo, Kanglai Tang, Taotao Liang
Biosensing platforms have become increasingly important in our daily lives due to their ability to provide real-time monitoring with low cost, high sensitivity, and specificity. Carbon nanotubes possess unique optical, electrical, and physical/chemical characteristics that can significantly enhance the electron transfer rate in biosensors, thereby improving sensing performance and enabling rapid detection
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Corrigendum to “Multiple and simultaneous detection for cytokines based on the nanohole array by electrochemical sandwich immunoassay” [Biosens. Bioelectron.: X 14 (2023) 100387] Biosens. Bioelectron. X Pub Date : 2023-12-07 Han Na Suh, Sung-Hoon Yoon, Yoo Min Park, HyungJin Kim, SangHee Kim, JuKyung Lee
Abstract not available
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Technologies for non-invasive physiological sensing: Status, challenges, and future horizons Biosens. Bioelectron. X Pub Date : 2023-12-05 Yang Yu, Bhavya Jain, Gautam Anand, Mahdi Heidarian, Andrew Lowe, Anubha Kalra
Non-invasive techniques have become increasingly vital in modern medicine, providing valuable diagnostic information without invasive procedures. These techniques encompass a diverse range of procedures, including imaging scans, blood tests, urine tests, and genetic testing, enabling the investigation of various conditions without device insertion. In contrast to conventional invasive methods, non-invasive
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Recent advances in multiplex aptasensor detection techniques for food-borne pathogens: A comprehensive review of novel approaches Biosens. Bioelectron. X Pub Date : 2023-11-19 Annu Mishra, Souradeep Roy, Naasrin Israel Shaikh, Pooja Malave, Ankita Mishra, Md. Anish Alam, Yashswee Ghorpade, Mohd. Rahil Hasan, Anam Nizam
Food-borne pathogens pose significant risks to public health and food safety, necessitating the development of rapid, sensitive, and multiplex detection techniques. Biosensor is one of the important tool to detect the analyte with high sensitivity. Now a days multiplex based approaches were in high demands due to its various advantages. One of the important advantages of multiplex aptasensor is it
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Atomic layer deposition-fabricated two-dimensional MoO3-WO3 nanohybrid thin-film electrode for ultrasensitive and interference-free detection of dopamine Biosens. Bioelectron. X Pub Date : 2023-11-18 Nasrin Siraj Lopa, Mohammad Karbalaei Akbari, Hong Liang Lu, Serge Zhuiykov
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Biosensors based on single or multiple biomarkers for diagnosis of prostate cancer Biosens. Bioelectron. X Pub Date : 2023-11-13 Yuanjie Teng, Wenhui Li, Sundaram Gunasekaran
Prostate cancer is the second deadliest cancer among men and poses a threat to the health of elderly men. Current methods of diagnosing prostate cancer including digital rectal tests or determining the increase in prostate-specific antigen level in serum are still not effective and hence can lead to overtreatment. New prostate cancer biomarkers in blood, urine, or tissues are reported and the methods
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Epitope imprinting of Sip D protein of Salmonella Typhi bacteria through multiple monomers approach Biosens. Bioelectron. X Pub Date : 2023-11-10 Ritu Singh, Mahesh L. Nair, Richa Raghuwanshi, Gopal Nath, Meenakshi Singh
Typhoid fever is an endemic disease in India. An early stage detection of this disease will reduce the burden on healthcare industry and prevent many fatalities. Here a MIP sensor is fabricated to detect Salmonella typhi bacteria through its antigenic epitope sequence identified through immunoinformatic tools. Epitope imprinting through multiple monomers was utilized to deposit the sensing matrix on
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A novel MB-tagged aptasensor for Aflatoxin B1 detection in food using Fe3O4 nanoparticles substantiated with in silico modelling Biosens. Bioelectron. X Pub Date : 2023-11-10 Lyndon Naidoo, Gloria Ebube Uwaya, Florian Meier, Krishna Bisetty
Aspergillus fungi species found in wheat, maize, rice, and other agricultural products produce the carcinogenic mycotoxin known as Aflatoxin B1 (AFB1), which can cause cancer in animals and humans. Consequently, recent interest has surged regarding the need for inexpensive, selective, and sensitive sensors to detect AFB1 in food. An ultrasensitive electrochemical aptasensor for AFB1 analysis was constructed
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Detection of anti-SARS CoV-2 antibodies in human serum by means of Bloch surface waves on 1D photonic crystal biochips Biosens. Bioelectron. X Pub Date : 2023-11-03 Agostino Occhicone, Alberto Sinibaldi, Daniele Chiappetta, Paola Di Matteo, Tommaso Pileri, Norbert Danz, Frank Sonntag, Peter Munzert, Matteo Allegretti, Valentina De Pascale, Chiara Mandoj, Francesco Michelotti
This study presents the development and characterization of a disposable biochip for the detection of antibodies against the SARS-CoV-2 spike protein, a well-known target for vaccine and therapeutic development. This biochip is based on a one-dimensional photonic crystal (1DPC) deposited on a plastic substrate and designed to sustain Bloch surface waves (BSW) in the visible range. The experimental
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Selective determination of methanol and ethanol using a sensor based on alcohol oxidase immobilized on a cassava biopolymer Biosens. Bioelectron. X Pub Date : 2023-10-29 Felipe Jadàn-Piedra, Jaime E. Cevallos-Mendoza, Juan Manuel Vera Delgado, Virginia Sánchez Mendoza, Mabel Laz Mero, Rodolfo Andrés Rivadeneira Z, Juan Antonio Dueñas Utreras, María Rodríguez-Gamez, Carlos Jadàn-Piedra
The analysis of alcohol represents an important parameter in food fermentation industries. The use of a biosensor would be an alternative to existing methods. An enzymatic sensor using alcohol oxidase (AOX) was used as the biorecognition element with an immobilized enzyme sensor in combination with an oxygen electrode, optimized in this study to determine the methanol content in cane brandy. The potentiometric
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A new biosensing platform based on L-cysteine-capped Fe3O4 nanoparticles embedded in chitosan-MWCNT matrix: Electrochemical kinetic and sensing studies Biosens. Bioelectron. X Pub Date : 2023-10-20 Tamal Sarkar, Nirmita Dutta, Gorachand Dutta
We present a report on the electrochemical kinetics and sensing studies conducted on a flexible ITO-PET transducer, fabricated with a new nanocomposite of L-cysteine coated iron oxide nanoparticles (LCys@Fe3O4 NPs) and multi-wall carbon nanotubes (MWCNT), for the first time. Synthesized via the wet-chemical co-precipitation method, the LCys@Fe3O4 NPs exhibit a pure cubic phase. A nanocomposite of these
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Advances in material for colorimetric and fluorescent detection of arsenic Biosens. Bioelectron. X Pub Date : 2023-10-17 Nishtha Khansili
Arsenic contamination in potable water has become a growing menace globally. The established method including instrumental and laboratory-based techniques can detect arsenic in water with an accuracy of 1 ppb. Nevertheless, these methods required trained expertise, laboratory set-up and installation cost. Thus, several material-based nanosensors are reported to propose a cost-effective and facile method
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Cell-based SELEX aptamer selection for electrochemical detection of Fluoribacter bozemanae bacteria Biosens. Bioelectron. X Pub Date : 2023-10-11 Aysha Shaukat, Amani Chrouda, Saima Sadaf, Fatimah Alhamlan, Khaled Al-Kattan, Mohammed Zourob
Fluoriobacter bozemanae (F. bozemanae) can cause Legionnaires' disease. The conventional methods for the detection of F. bozemanae are laborious, costly and has to be performed in centralized labs by skilled personnel. Therefore, there is an urgent need for rapid detection methods for F. bozemanae. The present work reports the first electrochemical aptasensor for the rapid and specific detection of
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Modelling of FinFET based ON bipolar cell of silicon retina Biosens. Bioelectron. X Pub Date : 2023-10-09 Payal Shah, Reena Sonkusare, Surendra S. Rathod
—In this paper, we proposed a SOI FinFET based ON bipolar cell of silicon retina which plays an important role in neuromorphic applications like differential motion detection. Low power consumption along with biological plausibility is one of the crucial parameters of any neuromorphic retinal circuit as in the brain all biological systems consisting of billions of neurons consume power in terms of
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Performance evaluation of ANFIS and RSM in modeling biodiesel synthesis from soybean oil Biosens. Bioelectron. X Pub Date : 2023-09-28 Sunil Kumar, Sumit Bansal
The parameters of biodiesel generation from Soybean oil having the molar ratio, reaction duration, and catalyst concentration for constant temperature, were modelled Using response surface methodology. a molar ratio of 6–12, NaOH of 1–2% w/w, time of 30–60 min, and temperature of 35–55 °C. an adaptive neuro-fuzzy inference system (ANFIS) and the response surface methodology-based Box–Behnken experimental
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Detection and counting of root-knot nematodes using YOLO models with mosaic augmentation Biosens. Bioelectron. X Pub Date : 2023-09-25 Top Bahadur Pun, Arjun Neupane, Richard Koech, Kerry Walsh
Root-knot nematodes (RKN) are microscopic plant parasites that cause significant economic damage to crops and vegetables. Accurate assessment of RKN populations is required for effective management of this disease. Several “You Only Look Once” (YOLO versions 2 to 7) architectures were investigated for the application of RKN enumeration in microscopic images. YOLOv5-608 model attained Precision score
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Sensing the impact of diet composition on protein fermentation by direct electrochemical NH4+ sensing in gastrointestinal digesta Biosens. Bioelectron. X Pub Date : 2023-09-21 Francesca Leonardi, Ria R. Sijabat, Roseanne Minderhoud, Aniek J.G. Even, Klaus Mathwig, Rachel E. Armstrong, Sonja de Vries, Annelies Goris, Chris van Hoof
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Laser induced graphene (LIG) biosensors derived from chitosan: Towards sustainable and green electronics Biosens. Bioelectron. X Pub Date : 2023-09-14 Hassan Hamidi, Juliette Levieux, Cathal Larrigy, Alida Russo, Eoghan Vaughan, Richard Murray, Aidan J. Quinn, Daniela Iacopino
Modern biosensors can provide real-time monitoring of individuals' health status, revolutionizing traditional healthcare diagnostics. As demand for these devices is continuously growing, the development of novel green materials and processes is mandatory to ensure sustainability. In this work, a simple laser direct writing approach was utilized to fabricate a novel green Laser Induced Graphene (LIG)
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Recent advances and challenges: Translational research of minimally invasive wearable biochemical sensors Biosens. Bioelectron. X Pub Date : 2023-09-12 Irfani R. Ausri, Yael Zilberman, Sarah Schneider, Xiaowu (Shirley) Tang
Wearable diagnostic devices that continuously monitor biomarkers of interest can significantly improve disease outcome. However, there is currently a lack of commercially available wearable biochemical health devices. Many of the commercially available devices are limited to monitoring the body's physical parameters. Even though there has been progress in the development of minimally invasive wearable
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Development of integrated smartphone/resistive biosensor for on-site rapid environmental monitoring of organophosphate pesticides in food and water Biosens. Bioelectron. X Pub Date : 2023-09-06 Hussian Maanaki, Terry Xu, Guibing Chen, Xiuxia Du, Jun Wang
Organophosphate (OP) pesticides remain a worldwide health concern due to their acute or chronic poisoning and widespread use in agriculture around the world. There is a need for robust and field-deployable tools for onsite detection of OP pesticides in food and water. Herein, we present an integrated smartphone/resistive biosensor for simple, rapid, reagentless, and sensitive monitoring of OP pesticides
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A highly sensitive electrochemical magneto-genosensing assay for the specific detection of a single nucleotide variation in the KRAS oncogene in human plasma Biosens. Bioelectron. X Pub Date : 2023-09-09 Simone Fortunati, Chiara Giliberti, Marco Giannetto, Alessandro Bertucci, Sabrina Capodaglio, Elena Ricciardi, Patrizio Giacomini, Valentina Bianchi, Andrea Boni, Ilaria De Munari, Roberto Corradini, Maria Careri
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Ultra-sensitive real-time detection of cancer-derived exosomes directly from cell supernatants by a large Goos–Hänchen signal generation on plasmonic sensing interface Biosens. Bioelectron. X Pub Date : 2023-08-22 Manel Hedhly, Yuye Wang, Aude Brunel, Flavien Beffara, Hussein Akil, Mireille Verdier, Barbara Bessette, Aurelian Crunteanu, Ho-Pui Ho, Georges Humbert, Fabrice Lalloue, Shuwen Zeng
Exosomes have shown great potential in serving as a cancer biomarker over these years since they carry crucial information of their parent cells. Therefore, detection of exosomes is of vital importance to the early-stage diagnostics of multiple major diseases. In this paper, we have proposed a real-time and label-free sensing technique based on 2D Ge2Sb2Te5 (GST) nanomaterial enhanced plasmonic substrate
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μDACS platform: A hybrid microfluidic platform using magnetic levitation technique and integrating magnetic, gravitational, and drag forces for density-based rare cancer cell sorting Biosens. Bioelectron. X Pub Date : 2023-08-26 Seren Kecili, Esra Yilmaz, Ozge Solmaz Ozcelik, Muge Anil-Inevi, Zehra Elif Gunyuz, Ozden Yalcin-Ozuysal, Engin Ozcivici, H. Cumhur Tekin
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Visualization of invisible cell-death sign by electric-double-layer modulation Biosens. Bioelectron. X Pub Date : 2023-08-16 Jun'ya Tsutsumi, Jun Kurosu, Kaname Kanai, Kingo Uchida, Kana Morishita, Kimio Sumaru
Leakage and self-spreading of a cytoplasmic lipid droplet in association with cell death, which was totally invisible in a conventional optical microscope, was discovered by using the recently developed electric-double-layer modulation imaging (EDLMI). The method allowed real-time imaging of the leaked lipid droplet with the thickness of a few nanometers. We demonstrate that the discovered phenomenon
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Biosensor for rapid and accurate detection of cardiovascular biomarkers: Progress and prospects in biosensors Biosens. Bioelectron. X Pub Date : 2023-08-11 Sophia Nazir, Rabail Azhar Iqbal
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Multiple and simultaneous detection for cytokines based on the nanohole array by electrochemical sandwich immunoassay Biosens. Bioelectron. X Pub Date : 2023-08-09 Han Na Suh, Sung-Hoon Yoon, Yoo Min Park, HyungJin Kim, SangHee Kim, JuKyung Lee
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Development of electrochemical immunosensor for quantitative detection of non-small cell lung cancer (NSCLC) biomaker YES1 Biosens. Bioelectron. X Pub Date : 2023-08-09 Lucia K. Kiio, John O. Onyatta, Peter M. Ndangili, Florence Oloo, Carolina Santamaría, Luis M. Montuenga, Damaris N. Mbui
Non-small cell lung cancer (NSCLC) is the most common type of lung cancer accounting for 85% of all newly diagnosed cases. Its prognosis remains poor as most patients are diagnosed at an advanced stage. In this study, we report the development of an electrochemical immunosensor for quantitative detection of Yamaguchi sarcoma viral oncogene homolog 1 (v-YES1) protein, comprised of a glassy carbon electrode
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Aptamer-based label-free electrochemical biosensors for the diagnosis of sickle cell anemia Biosens. Bioelectron. X Pub Date : 2023-08-08 Wjdan A. Arishi, Shimaa Eissa, Khaled Al-Kattan, Mohammed Zourob
Sickle cell Anemia (SCA) is a widespread disease that causes hemolysis of blood cells, anemia, painful episodes, organ damage, and in some cases death. Early diagnosis of sickle cell anemia is significantly useful to reduce the mortality rate and manage the disease effectively. Here, we report the selection and characterization of specific aptamers against hemoglobin A (HbA) and hemoglobin S (HbS)
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Colorimetric chemosensor for rapid detection of fluoroquinolone load in environmental water bodies, urine, and counterfeit drug testing Biosens. Bioelectron. X Pub Date : 2023-08-02 Tathagata Pal, Tennyson Mathai, Soumyo Mukherji
Fluoroquinolone is one of the most widely used class of broad-spectrum antibiotics in the world. Due to its widespread use and misuse, antimicrobial resistance against fluoroquinolones is becoming a matter of global health concern. Antibiotic residues are found in the surface and groundwater through the release of animal and human excretion and pharmaceutical effluents. Hence a robust and cost-effective
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A label-free electrochemical biosensor for the detection of alpha-thalassemia 1 (SEA deletion) carriers using screen-printed carbon electrodes Biosens. Bioelectron. X Pub Date : 2023-07-27 Areenuch Thamwarokun, Chollanot Kaset, Chanpen Karuwan, Wichayaporn Kamsong, Jirapat Attapong, Sirinart Chomean
A label-free electrochemical DNA biosensor based on electrochemical impedance spectroscopy (EIS) biosensor has been extensively developed for diagnosing human genetic diseases. However, its application has been limited to simulated target DNA. The aim of this study was to develop an EIS biosensor that can identify carriers of alpha (α)-thalassemia 1 with Southeast Asia (SEA) deletion. The biosensor
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Application of aptamer-based rapid screening test piece integrated face mask for wearer infected with virus Biosens. Bioelectron. X Pub Date : 2023-07-14
Coronavirus disease 2019 (COVID-19) has significantly affected human life, and preventing the spread of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has become an important issue. Nowadays, rapid screening tests are primarily used to identify potentially infected patients and prevent viral transmission. At present, commercially available antibody-based rapid screening tests require
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An affordable automated LED array system for optimizing photodynamic therapy protocols Biosens. Bioelectron. X Pub Date : 2023-07-16
Photodynamic therapy (PDT) is a promising minimally invasive alternative cancer treatment that, due to fluorescent properties of the drug (photosensitizer), can be used as a theragnostic agent. The efficacy of this treatment largely relies on the successful activation of the photosensitizer with appropriate light. While certain studies demonstrate comparable outcomes between LED and laser activation
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Novel, accurate pathogen sensors for fast detection of SARS-CoV-2 in the aerosol in seconds for a breathalyzer platform Biosens. Bioelectron. X Pub Date : 2023-07-13
Rapid and accurate detection of the pathogens, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) for COVID-19, is critical for mitigating the COVID-19 pandemic. Current state-of-the-art pathogen tests for COVID-19 diagnosis are done in a liquid medium and take 10–30 min for rapid antigen tests and hours to days for polymerase chain reaction (PCR) tests. Herein we report novel accurate
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Linker-mediated oriented antibody immobilisation strategies for a more efficient immunosensor and diagnostic applications: A review Biosens. Bioelectron. X Pub Date : 2023-07-08 Shin Yi Gan, Gee Jun Tye, Ai Lan Chew, Woei Kean Ng, Ngit Shin Lai
The conjugation of antibodies onto the solid surface in a well-oriented manner has a profound impact on the sensitivity and limit of detection of an immunoassay. The chosen method of immobilisation significantly influences the interaction between antibodies and antigens on the sensing surface. Optimal antigen capture capacity is achieved when the Fab fragments of the antibodies are properly oriented