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Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an In Vitro Model Based on Differentiated PC12 Cells Altern. Lab. Anim. (IF 2.7) Pub Date : 2024-03-06 Seyed Mobin Seyed Aliyan, Ali Roohbakhsh, Marzieh Jafari Fakhrabad, Zahar Salmasi, Mohammad Moshiri, Niosha Shahbazi, Leila Etemad
Methamphetamine (Meth) is a highly addictive stimulant. Its potential neurotoxic effects are mediated through various mechanisms, including oxidative stress and the initiation of the apoptotic process. Thymoquinone (TQ), obtained from Nigella sativa seed oil, has extensive antioxidant and anti-apoptotic properties. This study aimed to investigate the potential protective effects of TQ against Meth-induced
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A Cytotoxicity Assay as an Alternative to the Murine Model for the Potency Testing of Bothrops jararaca Venom and Antivenom: An Intralaboratory Pre-validation Study Altern. Lab. Anim. (IF 2.7) Pub Date : 2024-03-05 Renata N.C. Nundes, Antonio E.C.C. Almeida, Wlamir C. Moura, Marcelo S. Gonzalez, Humberto P. Araújo
Antivenom therapy is the only specific treatment for snakebite envenomation, and antivenom potency determination is key in the efficacy assurance quality control process. Nowadays, this process relies on the in vivo murine model — thus, the development of alternative in vitro methods is imperative. In the current study, the principle of the proposed method is the ability of Bothrops venom to induce
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Chitosan–Calcium Aluminate as a Cell-homing Scaffold: Its Bioactivity Testing in a Microphysiological Dental Pulp Platform Altern. Lab. Anim. (IF 2.7) Pub Date : 2024-02-14 Ester Alves Ferreira Bordini, Vitor de Toledo Stuani, Lígia Espoliar Correa, Fernanda Balestrero Cassiano, Marcella Fernandes Lovison, Maria Luisa Leite, Josimeri Hebling, Carlos Alberto de Souza Costa, Diana Gabriela Soares
In vitro models of the dental pulp microenvironment have been proposed for the assessment of biomaterials, to minimise animal use in operative dentistry. In this study, a scaffold/3-D dental pulp cell culture interface was created in a microchip, under simulated dental pulp pressure, to evaluate the cell-homing potential of a chitosan (CH) scaffold functionalised with calcium aluminate (the ‘CHAlCa
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Report of the First ONTOX Stakeholder Network Meeting: Digging Under the Surface of ONTOX Together With the Stakeholders Altern. Lab. Anim. (IF 2.7) Pub Date : 2024-01-18 Michael G. Diemar, Mathieu Vinken, Marc Teunis, Cyrille A.M. Krul, Francois Busquet, Julia Dominika Zajac, Helena Kandarova, Raffaella Corvi, Matteo Z. Rosso, Anastasiia Kharina, Louise Stab Bryndum, Michael Santillo, Denise Bloch, Olena Kucheryavenko, Demosthenes Panagiotakos, Vera Rogiers, Manon Beekhuijzen, Arianna Giusti, Abdulkarim Najjar, Carol Courage, Torben Koenig, Susanne Kolle, Harrie Boonen
The first Stakeholder Network Meeting of the EU Horizon 2020-funded ONTOX project was held on 13–14 March 2023, in Brussels, Belgium. The discussion centred around identifying specific challenges, barriers and drivers in relation to the implementation of non-animal new approach methodologies (NAMs) and probabilistic risk assessment (PRA), in order to help address the issues and rank them according
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The Numbers of Animals Used in Mexico for Scientific and Educational Purposes. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-11-25 Patricia Frías-Álvarez,Gustavo Ortiz-Millán
In Mexico, there are no official public and reliably reported data on the total number and species of non-human animals used for scientific purposes. The aim of the current study was to calculate the total numbers of animals used for scientific and educational purposes in Mexico, from January 2015 to October 2021, based on data requested from the National Institute of Transparency, Access to Information
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The 3Ranker: An AI-based Algorithm for Finding Non-animal Alternative Methods. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-10-21 Niels van Beuningen,Sinne Alkema,Nils Hijlkema,Brun Ulfhake,Rafael Frias,Merel Ritskes-Hoitinga,Wynand Alkema
The search for existing non-animal alternative methods for use in experiments is currently challenging because of the lack of both comprehensive structured databases and balanced keyword-based search strategies to mine unstructured textual databases. In this paper we describe 3Ranker, which is a fast, keyword-independent algorithm for finding non-animal alternative methods for use in biomedical research
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Barriers to the Use of Recombinant Bacterial Endotoxins Test Methods in Parenteral Drug, Vaccine and Device Safety Testing. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-10-19 Elizabeth Baker,Jessica Ponder,Johannes Oberdorfer,Ingo Spreitzer,Jay Bolden,Marine Marius,Thierry Bonnevay,Kristie Sullivan
The Bacterial Endotoxins Test (BET) is a critical safety test that is used to detect bacterial endotoxins, which are the major contributor to fever-inducing contamination risks known as pyrogens. All parenteral therapies, including every lot of injected drugs, vaccines, medical devices, must be tested for pyrogens to ensure patient safety. Bacterial endotoxins test methods were developed as a highly
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Optimisation of a Method for the Differentiation of Human Umbilical Cord-derived Mesenchymal Stem Cells Toward Renal Epithelial-like Cells. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-10-13 Rakhshinda Habib,Shumaila Fahim,Mohsin Wahid,Jahanara Ainuddin
Human umbilical cord-derived mesenchymal stem cells (hucMSCs) can differentiate into multiple cell lineages, but few methods have been developed to generate kidney lineage cells. Due to their human origin, pluripotent nature and immunomodulatory properties, these stem cells are attractive candidates for clinical applications such as the repair or regeneration of damaged organs. This study evaluated
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Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-10-05 Ikuya Tanabe,Kunitaka Yoshida,Shinkichi Ishikawa,Kanae Ishimori,Tsuneo Hashizume,Takayuki Yoshimoto,Takao Ashikaga
Chemical respiratory sensitisation is a serious health problem. However, to date, there are no validated test methods available for identifying respiratory sensitisers. The aim of this study was to develop an in vitro sensitisation test by modifying the human cell line activation test (h-CLAT) to detect respiratory sensitisers and distinguish them from skin sensitisers. THP-1 cells were exposed to
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Development of Two Korean IACUC Guidance Documents to Foster Implementation of the Three Rs. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-08-20 Gwi Hyang Lee,Heui-Jin Kim,Young-Shin Joo,Seung Yeon Kim,Barney Reed,Lynette A Hart,Byung In Choe
In Korea, Institutional Animal Care and Use Committees (IACUCs) have been legally required to apply the Three Rs principles (i.e. replacement, reduction and refinement) and undertake the ethical review of animal study protocols, since 2008. According to Korean law, each IACUC is required to appoint at least one lay member recommended by a non-governmental animal protection organisation, who participates
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Evaluating the Impact of Hair Care Product Exposure on Hair Follicle and Scalp Health. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-08-09 Ernest S Fung,Jillian A Parker,Andrew D Monnot
Human hair follicles traverse the epidermis and dermis, and are comprised of specialised cells including dermal papilla cells (DPCs). DPCs play a critical role in the development and growth of both hair and follicle structure. While exposure of DPCs to undiluted exogenous compounds is unlikely, exposure to diluted compounds is possible should dermal penetration occur. The goal of this study was to
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Modelling the Sorafenib-resistant Liver Cancer Microenvironment by Using 3-D Spheroids. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-08-09 Ece Sariyar,Zeynep Firtina Karagonlar
Liver cancer is the third leading cause of cancer-related mortality, and hepatocellular carcinoma (HCC) is the most common form of liver cancer, and it usually occurs in the setting of chronic liver disease and cirrhosis. For patients with advanced HCC, systemic treatment is the first choice - however, resistance occurs frequently. Sorafenib was the first tyrosine kinase inhibitor approved for advanced
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The Use of In Silico Methods to Identify and Assess Antigenic Regions Suitable for the Development of Peptide-based Pan-viral Vaccines. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-08-07 Shayana Saravanakumar,Jhinuk Chatterjee
The constant evolution of pathogenic viral variants and the emergence of new viruses have reinforced the need for broad-spectrum vaccines to combat such threats. The spread of new viral variants leading to epidemic and pandemic infection can be effectively contained, if broad-spectrum vaccines effective against the newer viral variants are readily available. The development of broad-spectrum, pan-neutralising
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The Safety Assessment of Cosmetic Perfumes by Using In Chemico and In Vitro Methods in Combination with GC-MS/MS Analysis. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-06-28 Markéta Dvořáková,Lada Svobodová,Marian Rucki,Václav Ševčík,Barbora Hošíková,Jan Chrz,Hana Bendová,Kristina Kejlová,Danuše Očadlíková,Marek Malý,Hana Kolářová,Marika Mannerström,Helena Kanďárová,Dagmar Jírová
Animal testing has been prohibited for the safety assessment of cosmetic ingredients or finished products. Thus, alternative non-animal methods, followed by confirmatory clinical studies on human volunteers, should be used as the sole legally acceptable approach within the EU. The safety assessment of cosmetic products requires the involvement of multiple scientific disciplines, including analytical
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Fenugreek Seed Extract Regulates Human Umbilical Vein Endothelial Cell Angiogenesis and Proliferation via the PI3K/Akt/Cyclin D1 Pathway. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-06-22 Samaneh Safarpour,Farshad Mirzavi,Farzad Rahmani,Fatemeh Forouzanfar,Hamid Reza Sadeghnia,Baratali Mashkani,Daryoush Hamidi Alamdari,Mohammad Soukhtanloo
The significance of angiogenesis in tumour progression has been widely documented. Hence, the identification of anti-angiogenic agents with fewer common side effects would be valuable in cancer therapy. In this study, we evaluated the anti-angiogenic and anti-proliferative effects of a hydro-alcoholic extract of fenugreek seed (HAEF) on human umbilical vein endothelial cells (HUVECs). Human umbilical
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Is UK Animal Research Governance Facing a 'New Normal'? Considering the Risks and Benefits of 'Going Online'. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-06-21 Kathleen Salter
Since a return to in-office working after the extensive disruption caused by the COVID-19 pandemic and associated challenges, many conversations are still being had with regard to what the 'new normal' might look like in professional spaces and networks, and what lessons can be learnt from long periods of remote working. The regulation of animal research practice in the UK is no exception here and
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Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022. Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-06-06 Nikita Narayan Naik,Bharadwaja Vadloori,Suresh Poosala,Pratima Srivastava,Sandra Coecke,Adrian Smith,Aysha Akhtar,Clive Roper,Sridhar Radhakrishnan,Balaji Bhyravbhatla,Madhujit Damle,Venkat Koushik Pulla,Johannes Hackethal,Reyk Horland,Albert P Li,Falguni Pati,Manu Smriti Singh,Paola Occhetta,Rohit Bisht,Prajakta Dandekar,Krishna Bhagavatula,Dasja Pajkrt,Michael Johnson,Tilo Weber,John Huang,Lisiena
Animal experimentation has been integral to drug discovery and development and safety assessment for many years, since it provides insights into the mechanisms of drug efficacy and toxicity (e.g. pharmacology, pharmacokinetics and pharmacodynamics). However, due to species differences in physiology, metabolism and sensitivity to drugs, the animal models can often fail to replicate the effects of drugs
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In Silico Prediction of Eye Irritation Using Hansen Solubility Parameters and Predicted pKa Values Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-05-15 Martin Andersson, Ulf Norinder, Swapnil Chavan, Ian Cotgreave
An in silico method has been developed that permits the binary differentiation between pure liquids causing serious eye damage or eye irritation, and pure liquids with no need for such classificati...
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Use of the Allium cepa Model to Assess the Cytogenotoxicity of Luffariella herdmani Marine Sponge Extract Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-04-26 Sashini U. Kuruppuarachchi, Uthpala A. Jayawardena, Varuni K. Gunathilake
Marine sponge extracts are known to contain potentially toxic compounds that have biological activities of possible pharmacological interest. Thus, it is vital that biological models are used for t...
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A Human Cell-based Assay to Assess the Induction of Vasculature Formation for Non-genotoxic Carcinogenicity Testing Purposes: A Pilot Study Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-04-26 Veera Hautanen, Tarja Toimela, Martin Paparella, Tuula Heinonen
The induction of vasculature formation is proposed to be a significant mechanism behind the non-genotoxic carcinogenicity of a chemical. The vasculature formation model used in this study is based ...
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The Application of New Approach Methodologies in Respiratory Disease Research: Their Role in Improving Translational Medicine from Bench to Bedside Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-04-17 Artur C.G. da Silva, Marize C. Valadares
The SARS-CoV-2 outbreak focused global attention on the shortcomings of the drug discovery process. It led to its acceleration in several areas, particularly in the processes associated with the de...
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Poor Translatability of Biomedical Research Using Animals — A Narrative Review Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-03-07 Lindsay J. Marshall, Jarrod Bailey, Manuela Cassotta, Kathrin Herrmann, Francesca Pistollato
The failure rate for the translation of drugs from animal testing to human treatments remains at over 92%, where it has been for the past few decades. The majority of these failures are due to unex...
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The 19th FRAME Annual Lecture, November 2022: Safer Chemicals and Sustainable Innovation Will Be Achieved by Regulatory Use of Modern Safety Science, Not by More Animal Testing Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-03-01 Julia H. Fentem
The decisions we make on chemical safety, for consumers, workers and the environment, must be based on the best scientific data and knowledge available. Rapid advances in biology, in cell-based tec...
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Key Challenges and Recommendations for In Vitro Testing of Tobacco Products for Regulatory Applications: Consideration of Test Materials and Exposure Parameters Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-02-23 Martha M. Moore, Irene Abraham, Mark Ballantyne, Holger Behrsing, Xuefei Cao, Julie Clements, Marianna Gaca, Gene Gillman, Tsuneo Hashizume, Robert H. Heflich, Sara Hurtado, Kristen G. Jordan, Robert Leverette, Damian McHugh, Jacqueline Miller-Holt, Gary Phillips, Leslie Recio, Shambhu Roy, Mariano Scian, Liam Simms, Daniel J. Smart, Leon F. Stankowski, Jr, Robert Tarran, David Thorne, Elisabeth Weber
The Institute for In Vitro Sciences (IIVS) is sponsoring a series of workshops to identify, discuss and develop recommendations for optimal scientific and technical approaches for conducting in vit...
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In Vitro Dengue Virus Inhibition by Aqueous Extracts of Aegle marmelos, Munronia pinnata and Psidium guajava Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-02-15 Kalani Gayathri Jayasekara, Preethi Soysa, Thusharie Sugandhika Suresh, Charitha Lakshini Goonasekara, Kamani Mangalika Gunasekera
Dengue is an arboviral (insect-transmitted) infection of global concern. Currently, there are still no specific dengue antiviral agents to treat the disease. Plant extracts have been used in tradit...
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A Short History of the Consideration of Sex Differences in Biomedical Research — Lessons for the In Vitro Community from Animal Models and Human Clinical Trials Altern. Lab. Anim. (IF 2.7) Pub Date : 2023-02-07 Helena Niobe Renate Gutleb, Arno Christian Gutleb
In recent decades, it has become clear that in many fields — such as drug development, particularly with regard to drug dosage and specific disease treatment — the sex of a patient must be taken in...
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In Silico Prediction of Human Clinical Pharmacokinetics with ANDROMEDA by Prosilico: Predictions for an Established Benchmarking Data Set, a Modern Small Drug Data Set, and a Comparison with Laboratory Methods Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-12-26 Urban Fagerholm, Sven Hellberg, Jonathan Alvarsson, Ola Spjuth
There is an ongoing aim to replace animal and in vitro laboratory models with in silico methods. Such replacement requires the successful validation and comparably good performance of the alternati...
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The Effects of Urolithin B and Auraptene on Quinolinic Acid-induced Toxicity in the SH-SY5Y Neuroblastoma Cell Line Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-12-22 Farzaneh Abbasinezhad-Moud, Farshad Mirzavi, Hassan Rakhshandeh, Reza Mohebbati, Fatemeh Forouzanfar, Mohammad Jalili-Nik, Nadia Azadi, Mehdi Sanati, Amir R. Afshari, Mohammad Soukhtanloo
The pathological accumulation of quinolinic acid (QA) is often associated with neuritis and neuronal cell death in several neurodegenerative diseases, through the overproduction of free radicals. U...
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Cardiac Transcription Regulators Differentiate Human Umbilical Cord Mesenchymal Stem Cells into Cardiac Cells Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-12-09 Shumaila Shafique, Syeda Roohina Ali, Shafiqa Naeem Rajput, Asmat Salim, Irfan Khan
Stem cell-based therapy presents an attractive alternative to conventional therapies for degenerative diseases. Numerous studies have investigated the capability of human umbilical cord-derived mes...
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The Current Status and Work of Three Rs Centres and Platforms in Europe* Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-12-02 Winfried Neuhaus, Birgit Reininger-Gutmann, Beate Rinner, Roberto Plasenzotti, Doris Wilflingseder, Joery De Kock, Tamara Vanhaecke, Vera Rogiers, Dagmar Jírová, Kristina Kejlová, Lisbeth E. Knudsen, Rasmus Normann Nielsen, Burkhard Kleuser, Vivian Kral, Christa Thöne-Reineke, Thomas Hartung, Giorgia Pallocca, Costanza Rovida, Marcel Leist, Stefan Hippenstiel, Annemarie Lang, Ida Retter, Stephanie
The adoption of Directive 2010/63/EU on the protection of animals used for scientific purposes has given a major push to the formation of Three Rs initiatives in the form of centres and platforms. ...
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Corrigendum to Case Studies Exemplifying the Transition to Animal Component-Free Cell Culture. Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-12-01
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Optimisation of Conditions for the Formation of Spheroids of Head and Neck Squamous Cell Carcinoma Cell Lines for Use as Animal Alternatives Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-10-20 Esther Tenschert, Johann Kern, Annette Affolter, Nicole Rotter, Anne Lammert
The use of in vitro 3-D cell culture models in cancer research has yielded substantial gains in knowledge on various aspects of tumour biology. Such cell culture models could be useful in the study...
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Biomedical Research on Substances of Abuse: The Italian Case Study Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-10-12 Chiara Magliaro, Arti Ahluwalia
Substances of abuse have the potential to cause addiction, habituation or altered consciousness. Most of the research on these substances focuses on addiction, and is carried out through observatio...
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The Capability Maturity Model as a Measure of Culture of Care in Laboratory Animal Science Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-10-11 Dulshara Sachini Amarasekara, Hasanthi Rathnadiwakara, Kalpani Ratnayake, Mangala Gunatilake, Vijay Pal Singh, Suresh Poosala
Culture of care in Laboratory Animal Science (LAS) refers to a commitment toward improving animal welfare, scientific quality, staff wellbeing, and transparency for all stakeholders, ensuring that ...
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Transparency in Non-Technical Project Summaries to Promote the Three Rs in Respiratory Disease Research Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-09-15 Martina Bonassera, Esther Clews, Kelly BéruBé
Non-Technical Project Summaries (NTS) are legal documents that were first introduced by the Directive 2010/63/EU to enhance transparency within scientific animal experimentation. Researchers intend...
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An Evaluation of Different Types of Peptone as Partial Substitutes for Animal-derived Serum in Vero Cell Culture Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-09-03 Chloe Lezin, Philippe Mauduit, Georges Uzan, Mohamed Essameldin Abdelgawad
Vero cells are one of the most frequently used cell types in virology. They can be used not only as a vehicle for the replication of viruses, but also as a model for investigating viral infectivity...
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Conference Diary Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-08-26
1. Webinar: Less traditional research animals
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Spotlight on Three Rs Progress Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-08-26
The implementation of personalised cancer treatments depends on the existence of clinically relevant systems to identify the optimal testing strategy for each patient according to the mutations present in the cancer cells. In the specific case of non-small cell lung carcinoma (NSCLC), which is responsible for a high proportion of cancer-related deaths in the USA, the absence of assays suitable to test
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Case Studies Exemplifying the Transition to Animal Component-free Cell Culture Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-08-19 Tilo Weber, Joachim Wiest, Stina Oredsson, Karen Bieback
Cell culture techniques are strongly connected with modern scientific laboratories and production facilities. Thus, choosing the most suitable medium for the cells involved is vital, not only direc...
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A History of Regulatory Animal Testing: What Can We Learn? Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-08-19 Doortje Swaters, Anne van Veen, Wim van Meurs, Janette Ellen Turner, Merel Ritskes-Hoitinga
The contemporary pharmaceutical industry is voicing growing concerns about the translatability and reproducibility of animal models. In addition, the usefulness of certain of the required regulator...
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Addressing Animal Welfare Issues in Fetal Blood Collection for Fetal Bovine Serum Production Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-08-18 Terence J. McCann, Carol Treasure
There is ethical debate over whether fetal calves suffer when their dam is slaughtered and fetal blood extracted by cardiac puncture for fetal bovine serum (FBS) production. Yet, the serum industry...
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Ciliary Beat Frequency: Proceedings and Recommendations from a Multi-laboratory Ring Trial Using 3-D Reconstituted Human Airway Epithelium to Model Mucociliary Clearance Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-08-07 Holger P. Behrsing, Adam Wahab, Lindsey Ukishima, Chad Grodi, Stefan Frentzel, Stephanie Johne, Shinkichi Ishikawa, Shigeaki Ito, Roman Wieczorek, Jessica Budde, Brian M. Keyser, Linsey E. Haswell, David Thorne, Emma Bishop, Damien Breheny, Xuefei Cao, Qin Qin, Vivek Patel
The use of reconstituted human airway (RHuA) epithelial tissues to assess functional endpoints is highly relevant in respiratory toxicology, but standardised methods are lacking. In June 2015, the ...
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Biobank Personnel — The Key to its Success Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-07-21 Anna Chróścicka, Angelika Paluch, Ilona Kalaszczyńska, Ilona Szabłowska-Gadomska
Resources from biobanks and biorepositories, such as human samples, are of increasing interest to specialists in various fields. However, whilst biobanks provide a crucial service, their efficient ...
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Spotlight on Three Rs Progress Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-07-11
Lung transplantation requires ABO blood group matching, which limits the transplant opportunities for some patients. A recent paper by Wang et al.1 showed how two enzymes could be combined to convert blood group A (ABO-A) lungs to blood group O (ABO-O) lungs, potentially improving ABO-incompatible lung transplantation. The team used FpGalNAc deacetylase and FpGalactosaminidase to remove the blood group
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The Use of Human Tissues for Research: What Investigators Need to Know Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-07-08 Marianna J. Bledsoe, William E. Grizzle
While laboratory animals are necessary for some aspects of the development of scientific and biomedical advances, including those of precision medicine, the use of human tissues is necessary in order to explore the findings and ensure that they are relevant to human systems. Many sources of human tissues exist, but researchers — particularly those making the transition from animal to human systems
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Editorial Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-07-08 Rita Seabra
In this year’s fourth issue of ATLA, we have an impressively diverse set of papers, including a 50th Anniversary Article that leads us to reconsider a few notions, and several other papers that will be included in our Virtual Special Collection on Biobanking and the Use of Human Tissues. We are also pleased to announce the recipients of the Michael Balls Award 2021. These delighted winners are the
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Conference Diary Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-07-08
1. Responsible Research Free Webinar — Practical guide to developing a 3Rs strategy
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Development of an Accessible Gene Expression Bioinformatics Pipeline to Study Driver Mutations of Colorectal Cancer Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-06-28 Lisa van den Driest, Caroline H. Johnson, Nicholas J.W. Rattray, Zahra Rattray
Colorectal cancer (CRC) is a global cause of cancer-related mortality driven by genetic and environmental factors which influence therapeutic outcomes. The emergence of next-generation sequencing technologies enables the rapid and extensive collection and curation of genetic data for each cancer type into clinical gene expression biobanks. We report the application of bioinformatics tools for investigating
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The Evolution of Regulatory Toxicology: Where is the Gardener? Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-06-24 Robert Landsiedel, Barbara Birk, Dorothee Funk-Weyer
There is a need for paradigm change in the methodology employed for toxicological testing and assessment. It could be said that this change is well on its way, through an evolutionary progress analogous to that of natural selection. Darwin’s Theory of Evolution has defined the idea of evolution and descendancy since the last third of the 19th century. Increasingly, this concept of ‘evolution’ is being
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The Perceptions of Students and Lecturers on the Use of Animals in Biomedical Science Undergraduate Education in Brazil Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-06-06 Paula S. Matos, Bruna dos Santos Rodrigues, Thaís de Oliveira Fernandes, Renato Ivan de Ávila, Marize C. Valadares
The use of animals in research and education is a controversial topic that has raised extensive debates. Undergraduate students (n = 404) and lecturers (n = 62) from biomedical science schools at the Federal University of Goiás (UFG) in the municipality of Goiânia, Jataí and Catalão, Goiás, Brazil, were asked about their knowledge and opinions on bioethics, the use and importance of animals in education
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Educators’ Views on the Use of Dissection and Dissection Alternatives in American Biology Classrooms Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-05-20 Pamela Osenkowski, Ignas Karaliunas, Merari Diorio
Animal dissection remains a common practice in American biology classrooms, despite the availability of dissection alternatives to study anatomy and physiology. Indeed, there is a growing body of evidence in the literature suggesting that the use of alternatives leads to the greater achievement of learning objectives, as compared to dissection. To better understand the current use of and attitudes
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The Use of Simulators for Teaching Practical Clinical Skills to Veterinary Students — A Review Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-05-19 Helen R. Braid
In the context of veterinary education, simulators are devices or sets of conditions aiming to imitate real patients and enable students to practice skills without the need for live animal use. Simulator use in veterinary education has increased significantly in recent years, allowing consistent practical teaching without reliance on clinical cases. This review examines the available literature regarding
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A Low-fidelity Simulator for the Development of Vascular Ligation Skills Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-05-18 Juan José Perez-Rivero, Ileana María Barbosa-Callejas, Lilia Delgado-Garduño, Lidia Rodríguez-Buitrón, Amira Eunice Lavalle-Avalos, José Antonio Herrera-Barragan
Training simulators can facilitate the acquisition and development of basic surgical skills, and they constitute a safe and humane method that does not harm animals in the process. The objective of this work was to create and evaluate a low-cost simulator to help undergraduate students of veterinary medicine acquire and practise vascular ligation skills. A training model was made by using easily accessible
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Evaluation of a Low-cost Renal Simulator for the Diagnostic Ultrasound Training of Veterinary Medicine Students Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-05-18 Angel R. Lozada-Gallegos, Irma Campero-Ruíz Velasco, Juan J. Pérez-Rivero
The acquisition of ultrasound diagnostic skills via training is important for undergraduate veterinary medical students. Unfortunately, commercial simulators are costly, which limits their use and makes it necessary to develop low-cost simulators for training purposes. The aim of this study was to evaluate the efficacy of an easily constructed, low-cost, high-fidelity renal simulator for use in diagnostic
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The Use of 3-D Models of Echocardiographic Imaging Planes for Teaching Echocardiography Techniques for Use in Dogs and Cats Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-05-17 Bruna N. da Costa, Marlos G. Sousa, Fernanda N. Tanji, Mariana Ulanin, Marcela Wolf, Simone T. O. Stedile
Echocardiography is an invaluable technique for the diagnosis of heart disease. The aim of this study was to develop 3-D models of healthy and diseased hearts of dogs and cats, and to evaluate their effectiveness in assisting veterinary undergraduates to understand echocardiographic imaging planes. Resin models depicting the main echocardiographic imaging planes of normal hearts were created, as well
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Editorial Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-05-17 Susan Trigwell
This third issue of ATLA’s 50th Anniversary volume features a collection of papers that focus on the topic of humane teaching approaches. These papers will form an online Virtual Special Collection on the topic that will also feature some relevant papers from the ATLA archive. The ATLA Virtual Special Collections (see https://journals.sagepub.com/page/atl/virtualspecialcollections) aim to bring together
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The Use of Simulators for Teaching Practical Clinical Skills to Veterinary Students - A Review. Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-05-19 Helen R Braid
In the context of veterinary education, simulators are devices or sets of conditions aiming to imitate real patients and enable students to practice skills without the need for live animal use. Simulator use in veterinary education has increased significantly in recent years, allowing consistent practical teaching without reliance on clinical cases. This review examines the available literature regarding
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Evaluation of a Low-cost Renal Simulator for the Diagnostic Ultrasound Training of Veterinary Medicine Students. Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-05-18 Angel R Lozada-Gallegos,Irma Campero-Ruíz Velasco,Juan J Pérez-Rivero
The acquisition of ultrasound diagnostic skills via training is important for undergraduate veterinary medical students. Unfortunately, commercial simulators are costly, which limits their use and makes it necessary to develop low-cost simulators for training purposes. The aim of this study was to evaluate the efficacy of an easily constructed, low-cost, high-fidelity renal simulator for use in diagnostic
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A Low-fidelity Simulator for the Development of Vascular Ligation Skills. Altern. Lab. Anim. (IF 2.7) Pub Date : 2022-05-18 Juan José Perez-Rivero,Ileana María Barbosa-Callejas,Lilia Delgado-Garduño,Lidia Rodríguez-Buitrón,Amira Eunice Lavalle-Avalos,José Antonio Herrera-Barragan
Training simulators can facilitate the acquisition and development of basic surgical skills, and they constitute a safe and humane method that does not harm animals in the process. The objective of this work was to create and evaluate a low-cost simulator to help undergraduate students of veterinary medicine acquire and practise vascular ligation skills. A training model was made by using easily accessible