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Marine leech parasitism of sea turtles varies across host species, seasons, and the tumor disease fibropapillomatosis Dis. Aquat. Org. (IF 1.368) Pub Date : 2021-01-14 Leah T. Rittenburg; Jake R. Kelley; Kate L. Mansfield; Anna E. Savage
ABSTRACT: Fibropapillomatosis (FP) is a tumorous disease affecting all species of sea turtles and is associated with the pathogen chelonid alphaherpesvirus 5 (ChHV5). Hypothesized ChHV5 vectors include the marine leeches Ozobranchus branchiatus and O. margoi, but data on their associations with FP and ChHV5 are minimal. To establish relationships between leech parasitism, turtle hosts, and FP, we compared
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Causes of cetacean stranding and death on the Catalonian coast (western Mediterranean Sea), 2012-2019 Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 María Cuvertoret-Sanz; Carlos López-Figueroa; Alicia OByrne; Albert Canturri; Bernat Martí-Garcia; Ester Pintado; Lola Pérez; Llilianne Ganges; Alex Cobos; María Lourdes Abarca; Juan Antonio Raga; Marie-François Van Bressem; Mariano Domingo
ABSTRACT: The causes of cetacean stranding and death along the Catalan coast between 2012 and 2019 were systematically investigated. Necropsies and detailed pathological investigations were performed on 89 well-preserved stranded cetaceans, including 72 striped dolphins Stenella coeruleoalba, 9 Risso’s dolphins Grampus griseus, 5 bottlenose dolphins Tursiops truncatus, 1 common dolphin Delphinus delphis
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Locality, time and heterozygosity affect chytrid infection in yellow-bellied toads Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 Pia Oswald; Ariel Rodríguez; Johara Bourke; Norman Wagner; Nicole de Buhr; Holger Buschmann; Maren von Köckritz-Blickwede; Heike Pröhl
ABSTRACT: The chytrid fungus Batrachochytrium dendrobatidis (Bd) infects numerous amphibian species worldwide and is suggested to drive population declines and extinction events. We report a study of Bd infection at the northernmost distribution of the European yellow-bellied toad Bombina variegata. A total of 577 individuals from ponds in 16 study sites were sampled for DNA and Bd throughout the breeding
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Association between ALFPm3 single nucleotide polymorphism and white spot syndrome virus resistance in black tiger shrimp Penaeus monodon Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 Chettupon Pooljun; Pitchanee Jariyapong; Sataporn Direkbusarakom; Sudarat Saeng-ngern; Takashi Sakamoto; Suwit Wuthisuthimethavee
ABSTRACT: Here single nucleotide polymorphisms (SNPs) were associated with white spot syndrome virus (WSSV) resistance in black tiger shrimp Penaeus monodon. SNPs were identified by single-strand conformation polymorphism (SSCP) screening and DNA sequencing of shrimp sampled from 3 families (100 shrimp per family) challenged with WSSV. Shrimp that died over the 14 d challenge trial were designated
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Nanopore whole genome sequencing and partitioned phylogenetic analysis supports a new salmonid alphavirus genotype (SAV7) Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 Andrew J. Tighe; Michael D. Gallagher; Jens Carlsson; Iveta Matejusova; Fiona Swords; Daniel J. Macqueen; Neil M. Ruane
ABSTRACT: Salmon pancreas disease virus, more commonly known as salmonid alphavirus (SAV), is a single-stranded positive sense RNA virus and the causative agent of pancreas disease and sleeping disease in salmonids. In this study, a unique strain of SAV previously isolated from ballan wrasse was subjected to whole genome sequencing using nanopore sequencing. In order to accurately examine the evolutionary
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Streptococcus iniae associated mass marine fish kill off Western Australia Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 Erina J. Young; Jo Bannister; Nicky B. Buller; Rebecca J. Vaughan-Higgins; Nahiid S. Stephens; Scott D. Whiting; Lian Yeap; Terrence L. Miller; Kristin S. Warren
ABSTRACT: Streptococcus iniae causes high mortality in cultured and wild fish stocks globally. Since the first report in captive Amazon river dolphins Inia geoffrensis in 1976, it has emerged in finfish across all continents except Antarctica. In March 2016, an estimated 17000 fish were observed dead and dying along a remote 70 km stretch of the Kimberley coastline north of Broome, Western Australia
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Gas embolism and massive blunt force trauma to sea turtles entrained in hopper dredges in North and South Carolina, USA Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 Craig A. Harms; Shane M. Boylan; Brian A. Stacy; Jean F. Beasley; Daniel García-Párraga; Matthew H. Godfrey
ABSTRACT: Decompression sickness (DCS) has been described mainly in loggerhead turtles Caretta caretta bycaught in trawls and gillnets. Here we present cases of gas emboli (GE) in 8 green turtles Chelonia mydas and 2 Kemp’s ridleys Lepidochelys kempii entrained in hopper dredges that were working at 8.8-15.2 m depths during shipping channel maintenance or beach renourishment activities. Turtle weights
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Differential liver histopathological responses to amphibian chytrid infection Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 Raquel F. Salla; Monica Jones-Costa; Fabio C. Abdalla; Felipe A. P. Vidal; Guilherme A. N. S. Boeing; Cristiane R. Oliveira; Elaine C. M. Silva-Zacarin; Lilian Franco-Belussi; Gisele M. Rizzi-Possignolo; Carolina Lambertini; Luís F. Toledo
ABSTRACT: Amphibians have been facing a pandemic caused by the deadly fungus Batrachochytrium dendrobatidis (Bd). Although studies have elucidated cutaneous and homeostatic disturbances, it is still unknown if the hepatic function can be affected or if hepatic effects differ among host species. Thus, we evaluated the effects of an experimental Bd infection on the liver (histopathology and the hepatosomatic
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Seasonal prevalence of the amphibian chytrid in a tropical pond-dwelling tadpole species Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 Joice Ruggeri; Ana Glaucia Da Silva Martins; Adão Henrique Rosa Domingos; Isaias Santos; Imran Bharat Viroomal; Luís Felipe Toledo
ABSTRACT: Infection by the chytrid fungus Batrachochytrium dendrobatidis (Bd) is a major threat to amphibians and has caused catastrophic global declines of amphibian populations. Some studies have detected a seasonal pattern of infection associated with the local climate, and although most of them have focused on investigating the seasonality of Bd in relation to its impacts on amphibians, fewer have
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Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004 Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 Angélica Maria Sánchez-Sarmiento; Valeria Ruoppolo; Mônica Mathias Costa Muelbert; José Soares Ferreira Neto; José Luiz Catão-Dias
ABSTRACT: Brucella spp. and Leptospira spp. antibodies were surveyed in 35 southern elephant seals (SESs) Mirounga leonina at Elephant Island (South Shetland Islands), western Antarctic peninsula, in the Austral summer of 2003 and 2004. The rose Bengal test and a commercial competitive ELISA (c-ELISA) were used to detect Brucella spp. exposure, and the microscopic agglutination test (MAT) with 22 live
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Efficacy of nano zinc oxide dietary supplements on growth performance, immunomodulation and disease resistance of African catfish Clarias gariepinus Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-17 Heba H. Mahboub; Khalid Shahin; Asmaa W. Zaglool; Elshimaa M. Roushdy; Shaimaa A. A. Ahmed
ABSTRACT: Zinc (Zn) is an important trace element in fish diets that is required for growth, immunity and antioxidant defense mechanisms. The current study assessed the effects of both organic and nanoparticle zinc oxide (ZnO and ZnO-NPs, respectively) on growth performance, immune response and the antimicrobial effect against Pseudomonas aeruginosa in African catfish Clarias gariepinus. Fish were
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Defining the aetiology of amoebic diseases of aquatic animals: trends, hurdles and best practices Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-03 Chloe J. English; Paula C. Lima
ABSTRACT: Disease caused by parasitic amoebae impacts a range of aquatic organisms including finfish, crustaceans, echinoderms and molluscs. Despite the significant economic impact caused in both aquaculture and fisheries, the aetiology of most aquatic amoebic diseases is uncertain, which then affects diagnosis, treatment and prevention. The main factors hampering research effort in this area are the
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An outbreak of multidrug-resistant Salmonella Kentucky infection in long-nosed fur seals Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-03 David J. Blyde; Duan March; Peter Howard; Vitali Sintchenko; Estelle Rousselet; Cathrine Atkin
ABSTRACT: An outbreak of salmonellosis occurred in a group of 7 long-nosed fur seals Arctocephalus forsteri undergoing rehabilitation after being found injured and malnourished on beaches along the northern New South Wales and southern Queensland coasts of Australia. Three of the 7 individuals developed clinical disease and died within 3 d. Clinical signs included profuse diarrhea, vomiting, depression
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Exposure of the Gulf of St. Lawrence grey seal Halichoerus grypus population to potentially zoonotic infectious agents Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-03 Caroline C. Sauvé; Adrián Hernández-Ortiz; Emily Jenkins; Fabien Mavrot; Angela Schneider; Susan Kutz; Jeremiah T. Saliki; Pierre-Yves Daoust
ABSTRACT: The population of grey seals Halichoerus grypus in Canadian waters is currently used as a commercial source of meat for human consumption. As with domestic livestock, it is important to understand the occurrence in these seals of infectious agents that may be of public health significance and thus ensure appropriate measures are in place to avoid zoonotic transmission. This study examined
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Surveying for Batrachochytrium salamandrivorans presence in Spanish captive collections of amphibians Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-12-03 D. Lastra González; V. Balá; P. Chajma; J. Vojar
ABSTRACT: Batrachochytrium salamandrivorans (Bsal), a pathogenic fungus causing the fatal disease chytridiomycosis in amphibians, was likely introduced to Europe through the trade in pet salamanders from Asia and then escaped into wild populations. Among European countries, Spain has a large number of private breeders and keepers of pet salamanders, and cases of Bsal in wild and captive populations
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Ellipsomyxa ariusi sp. nov. (Myxosporea: Ceratomyxidae), a new myxosporean infecting the gallbladder of threadfin sea catfish Arius arius in India Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-11-19 Archana Chandran; P. U. Zacharia; T. V. Sathianandan; N. K. Sanil
ABSTRACT: The present study describes a new species of myxosporean, Ellipsomyxa ariusi sp. nov., infecting the gallbladder of the threadfin sea catfish Arius arius (Hamilton, 1822). E. ariusi sp. nov. is characterized by bivalvular, ellipsoid or elongate-oval myxospores with smooth spore valves and a straight suture, arranged at an angle to the longitudinal spore axis. Mature myxospores measured 10
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Morphological, histological, and molecular aspects of Myxobolus zaikae n. sp., a parasite of the roach Rutilus rutilus, in Lake Baikal Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-11-19 Marina Dashi-Dorjievna Batueva
ABSTRACT: A new myxobolid species, Myxobolus zaikae n. sp., was found in the connective tissue near the kidney and liver blood vessels of the common roach Rutilus rutilus, while fish myxosporean fauna were being investigated in Lake Baikal, Russia. The parasites were studied on the basis of spore morphology as well as with histological and molecular methods. Mature spores of M. zaikae n. sp. are round
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Cytology reveals diverse cell morphotypes and cellin-cell interactions in normal collector sea urchins Tripneustes gratilla Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-11-19 Thierry M. Work; Elena Millard; Daniela B. Mariani; Tina M. Weatherby; Robert A. Rameyer; Julie Dagenais; Renee Breeden; Allison M. Beale
ABSTRACT: Echinoderms such as sea urchins are important in marine ecosystems, particularly as grazers, and unhealthy sea urchins can have important ecological implications. For instance, unexplained mortalities of Diadema antillarum in the Caribbean were followed by algal overgrowth and subsequent collapse of coral reef ecosystems. Unfortunately, few tools exist to evaluate echinoderm health, making
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Pulmonary hair embolism in a rescued free-ranging Eurasian otter Lutra lutra in Kinmen, Taiwan Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-11-19 Wen-Ta Li; Yi-Lun Chiang; Ting-Yu Chen; Chiao-Ling Lai
ABSTRACT: Eurasian otters Lutra lutra are listed as Near Threatened on the IUCN Red List and are imperiled by habitat loss, water pollution, and poaching. Harassment and attacks by stray animals are also recognized threats to the health of wild Eurasian otters. Pulmonary hair embolism is a possible complication in animals with deep traumatic injury, but to date no cases have been reported in wildlife
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Emamectin benzoate is a safe and effective anthelmintic against coelomic nematode Philometra rubra in striped bass Morone saxatilis Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-11-19 Karine Béland; Guylaine Séguin; Stéphane Lair
ABSTRACT: An unusually high mortality rate due to verminous (Philometra rubra) coelomitis was documented in wild-hatched striped bass Morone saxatilis raised in a fish hatchery as part of a stock restoration program. To decrease the parasitic burden and therefore potentially minimize mortality, the effectiveness of 2 different anthelmintics was evaluated. Two trials were conducted on wild-collected
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Campylobacter pinnipediorum subsp. caledonicus and C. pinnipediorum subsp. pinnipediorum recovered from abscesses in pinnipeds Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-11-19 Geoffrey Foster; Johanna L. Baily; Fiona Howie; Andrew C. Brownlow; Jaap A. Wagenaar; Maarten J. Gilbert; William G. Miller; Barbara A. Byrne; Kristin A. Clothier; Todd Schmitt; Tony Patterson; Robert J. Reid; Mark P. Dagleish
ABSTRACT: Campylobacter pinnipediorum was described recently for isolates recovered from pinnipeds. The novel species was further split into 2 subspecies based on host and geography, with C. pinnipediorum subsp. pinnipediorum recovered from otariid seals in California (USA) and C. pinnipediorum subsp. caledonicus recovered from phocid seals in Scotland. We report details of the infections of 7 pinnipeds
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Tuberculosis in wild South American sea lions Otaria flavescens stranded in Chubut, Argentina Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-11-19 Carla Fiorito; Jimena Marfil; Elvira Falzoni; Marcela Martínez Vivot; Martín Zumárraga; Daniel Lombardo; Soledad Barandiaran
ABSTRACT: Pinniped tuberculosis, commonly caused by Mycobacterium pinnipedii, is a zoonotic disease reported in free-living and captive otariid species of the southern hemisphere. Currently, data concerning pinniped tuberculosis in South America are scarce, reinforcing the need for further studies of the disease in free-ranging pinnipeds. In this study, we investigated the presence of tuberculosis
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Ingestion of foreign materials by odontocetes along the Catalan coast: causes and consequences Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-11-05 A. Lacombe; E. Pintado; A. OByrne; A. Allepuz; L. Pérez-Rodriguez; M. Domingo
ABSTRACT: Ingestion of abnormal materials by cetaceans has been reported worldwide, but few studies have investigated the causes of foreign material ingestion. We retrospectively analysed necropsies performed between 2012 and 2019 on 88 cetaceans stranded along the coast of Catalonia, Spain, and evaluated the association of abnormal ingested materials with 2 risk factors, namely disease of the central
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Nocardia seriolae: a serious threat to the largemouth bass Micropterus salmoides industry in Southwest China Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-11-05 Xueping Lei; Ruoxuan Zhao; Yi Geng; Kaiyu Wang; Ping Ou Yang; Defang Chen; Xiaoli Huang; Zhicai Zuo; Changliang He; Zhengli Chen; Chao Huang; Hongrui Guo; Weimin Lai
ABSTRACT: Nocardia seriolae is the causative agent of nocardiosis in both marine and freshwater fish. Here, we report on multiple outbreaks of nocardiosis associated with elevated mortality (23-35%) in farmed largemouth bass in Sichuan, China, from 2017 to 2018. A total of 9 strains isolated from diseased largemouth bass were identified as N. seriolae by phenotypic characterization, 16S rRNA and hsp65
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Occurrence of Brucella ceti in stranded bottlenose dolphins Tursiops truncatus coincides with calving season Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-10-22 Wayne E. McFee; David Wu; Kathleen Colegrove; Karen Terio; Len Balthis; Robert Young
ABSTRACT: Brucellosis is a disease caused by the Gram-negative facultative intracellular bacterium Brucella spp. In terrestrial species, this zoonotic bacterium is a global public health risk, but there is also concern over the zoonotic potential of marine forms, such as B. ceti, which affects cetaceans. Due to the detection of B. ceti in samples from bottlenose dolphins Tursiops truncatus during the
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Validation of an eDNA-based method for the detection of wildlife pathogens in water Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-10-22 Natalie Sieber; Hanna Hartikainen; Christoph Vorburger
ABSTRACT: Monitoring the occurrence and density of parasites and pathogens can identify high infection-risk areas and facilitates disease control and eradication measures. Environmental DNA (eDNA) techniques are increasingly used for pathogen detection due to their relative ease of application. Since many factors affect the reliability and efficacy of eDNA-based detection, rigorous validation and assessment
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Helicobacter delphinicola sp. nov., isolated from common bottlenose dolphins Tursiops truncatus with gastric diseases Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-10-08 Takao Segawa; Yoshito Ohno; Sayaka Tsuchida; Kazunari Ushida; Motoi Yoshioka
ABSTRACT: Gastritis and gastric ulcers are well-recognized symptoms in cetaceans, and the genus Helicobacter is considered as the main cause. In this study, we examined the gastric fluid of captive common bottlenose dolphins Tursiops truncatus with gastric diseases in order to isolate the organisms responsible for diagnosis and treatment. Four Gram-negative, rod-shaped isolates (TSBT, TSH1, TSZ, and
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Susceptibility of five different sizes of pathogenfree Litopenaeus vannamei to white spot syndrome virus (WSSV) by intramuscular inoculation. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-24 Shengyu Liao,Ruihang Xu,Hanxi Wu,Dianjiang Yu,Shuangyan Wei,Peimin He,Rui Jia
ABSTRACT: White spot syndrome virus (WWSV) has become one of the most widespread causes of mortality in commercial shrimp farming. In the present study, we used PCR to determine the shrimp infectious dose 50% endpoint (SID50 ml-1) of a Chinese isolate of WSSV in 5 different sizes of pathogen-free Litopenaeus vannamei inoculated intramuscularly. The lethal dose 50% endpoint (LD50 ml-1) was also determined
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Choice of molecular assay determines ranavirus detection probability and inferences about prevalence and occurrence. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-24 Felicity J Wynne,Robert Puschendorf,Mairi E Knight,Stephen J Price
ABSTRACT: Ranaviruses are emerging pathogens that can cause morbidity, mortality and population declines in ectothermic hosts; however, there is no standardized approach to diagnostics. Here, we compared the inter-assay variation and intra-assay precision among 2 commonly used quantitative PCRs (qPCRs), a conventional and a nested PCR assay (used as a gold standard), using laboratory-propagated ranavirus
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Experimental transmission of Caligus rogercresseyi between two different fish species. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-24 M P González-Gómez,L Ovalle,C Spinetto,C Oyarzo,R Oyarzún,M Menanteau,D Álvarez,M Rivas,P Olmos
ABSTRACT: Caligus rogercresseyi is the dominant sea louse parasite affecting the salmon and trout industry in southern Chile. This parasite has a wide range of native and endemic fish hosts. The Patagonian blenny Eleginops maclovinus, which is parasitized mostly by the caligid species Lepeophtheirus spp. and C. rogercresseyi, is presumably responsible for the transmission of C. rogercresseyi to salmonids
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Shell disease does not affect biochemical profiles of the North Sea brown shrimp Crangon crangon. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-24 Alexandra Segelken-Voigt,Wilhelm Hagen,Gabriele Gerlach,Julia Strahl
ABSTRACT: The brown shrimp Crangon crangon is a key component of the North Atlantic coastal food web and an important target species for the fishery economy. As the brown shrimp contains large amounts of protein and essential fatty acids, its consumption makes it a beneficial choice for humans. Commercially harvested crustaceans like C. crangon are frequently affected by bacterial shell disease, with
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Susceptibility of rainbow trout to three different genogroups of infectious pancreatic necrosis virus. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-24 Anna Maria Eriksson-Kallio,Riikka Holopainen,Perttu Koski,Antti Nousiainen,Heikki Koskinen,Antti Kause,Tuija Gadd
ABSTRACT: Infectious pancreatic necrosis (IPN) is a globally distributed viral disease that is highly prevalent in rainbow trout Oncorhynchus mykiss farms in Finland. Seven genogroups (1-7) of infectious pancreatic necrosis virus (IPNV) exist, of which genogroup 5 (serotype Sp) is generally considered to be the most virulent in European salmonid farming. In Finland, 3 genogroups (2, 5 and 6) have been
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PCR-RFLP: a targeted method to reveal host specific malacosporean infection profiles (Cnidaria: Myxozoa: Malacosporea). Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-17 Paolo Ruggeri,Juliana Naldoni,Hanna Hartikainen,Beth Okamura
ABSTRACT: Malacosporeans are a group of endoparasitic cnidarians (Myxozoa) that use freshwater bryozoans and fish as final and intermediate hosts, respectively. The malacosporean Tetracapsuloides bryosalmonae causes proliferative kidney disease (PKD), an emerging disease in aquaculture and wild fish populations, including threatened salmonids in Europe and the USA. Mixed infections of malacosporeans
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Pathology of multifocal purple spots, a nonspecific lesion morphology of Caribbean sea fans Gorgonia spp. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-17 Michelle M Dennis,Anne A M J Becker,Mark A Freeman
ABSTRACT: Disease is contributing to the decline of coral reefs globally, but the cause and pathogenesis of most coral diseases are poorly understood. Using Gorgonia ventalina and G. flabellum as a model for coral disease diagnosis, we histologically and microbiologically examined 45 biopsies of lesions resembling Gorgonia multifocal purple spots (MFPS) with the aim of forming a comprehensive case
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Evidences supporting Enterocytozoon hepatopenaei association with white feces syndrome in farmed Penaeus vannamei in Venezuela and Indonesia. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-17 Luis Fernando Aranguren Caro,Hung N Mai,Orlando Pichardo,Roberto Cruz-Flores,Bambang Hanggono,Arun K Dhar
ABSTRACT: White feces syndrome (WFS) is an emerging and poorly described disease characterized by the presence of floating white fecal strings in shrimp (Penaeus monodon and P. vannamei) grow-out ponds. WFS has been associated with several pathogens, including Enterocytozoon hepatopenaei. This association is based on the fact that in areas where E. hepatopenaei has been reported, there was also a high
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Multilocus sequence analysis of diverse Streptococcus iniae isolates indicates an underlying genetic basis for phenotypic heterogeneity. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-17 Taylor I Heckman,Matt J Griffin,Alvin C Camus,Benjamin R LaFrentz,Danny Morick,Rita Smirnov,Tamir Ofek,Esteban Soto
ABSTRACT: Streptococcus iniae is a Gram-positive, opportunistically zoonotic bacterium infective to a wide variety of farmed and wild fish species worldwide. Outbreaks in wild fish can have detrimental environmental and cultural impacts, and mortality events in aquaculture can result in significant economic losses. As an emerging or re-emerging pathogen of global significance, understanding the coalescing
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Negative impact on the reproductive potential of blue jack mackerel Trachurus picturatus by Kudoa infection of the ovary. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-17 A Neves,I Gordo,V Sequeira,A R Vieira,E Silva,F Silva,A M Duarte,S Mendes,R Ganhão,M C Peleteiro,C Assis,R Rebelo,M F Magalhães,M M Gil,L S Gordo
ABSTRACT: Reproduction of Trachurus picturatus off the western Portuguese coast was studied over 1 yr. During histological analyses, the presence of Kudoa sp. was detected in advanced vitellogenic oocytes. Kudoa infections are known to cause economic loss through the induction of post-mortem liquefaction of fish muscles, but ovarian infection as reported in this study will seriously affect the reproductive
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Morphological, histological and molecular characterization of Myxidium cf. rhodei infecting the kidney of Rutilus rutilus. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-17 Marina Dashi-Dorjievna Batueva,Xiaoyi Pan,Jinyong Zhang,Xinhua Liu,Wu Wei,Yang Liu
ABSTRACT: In the present study, we provide supplementary data for Myxidium cf. rhodei Léger, 1905 based on morphological, histological and molecular characterization. M. cf. rhodei was observed in the kidneys of 918 out of 942 (97%) roach Rutilus rutilus (Linnaeus, 1758). Myxospores of M. cf. rhodei were fusiform with pointed ends, measuring 12.7 ± 0.1 SD (11.8-13.4) µm in length and 4.6 ± 0.1 (3.8-5
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Pathologic findings and causes of death in bottlenose dolphins Tursiops truncatus stranded along the Georgia coast, USA (2007-2013). Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-17 M Seguel,R C George,G Maboni,S Sanchez,A Page-Karjian,E Wirth,W McFee,N L Gottdenker
ABSTRACT: Between 2007 and 2013, before the 2013 cetacean morbillivirus outbreak, 26 fresh bottlenose dolphin carcasses were necropsied on the coast of Georgia, USA. Here, we present the pathological and microbiological findings associated with their most likely causes of death. The primary cause of death was determined in 25 individuals and included systemic bacterial infection (n = 7), verminous
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Longitudinal survey of Flavobacterium species in Icelandic salmonid fish farms. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-17 Thorbjorg Einarsdottir,Gudbjorg Guttormsdottir,Daniel Connaghan,Sigridur Hjartardottir
ABSTRACT: Flavobacterium species cause significant disease in salmonid farming worldwide, typically seen as mortality in sac fry and later as necrosis and ulceration in fingerlings and fry. In this study, we sampled Atlantic salmon Salmo salar and Arctic char Salvelinus alpinus from 5 Icelandic fish farms in 2014 and 2017, where flavobacteria were suspected to cause disease. The objective of the study
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Carvacrol essential oil stimulates growth performance, immune response, and tolerance of Nile tilapia to Cryptococcus uniguttulatus infection. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-17 Heba H Mahboub,Yasmine H Tartor
ABSTRACT: This study investigated the antifungal activity of 5 essential oils (EOs) towards yeasts recovered from diseased fishes; and focused on the efficacy of one EO (carvacrol) on growth performance, non-specific immunity, and disease resistance of Nile tilapia Oreochromis niloticus against Cryptococcus uniguttulatus challenge. Thymoquinone, thymol, carvacrol, eugenol, and cinnamon were first tested
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First detection of infectious haematopoietic necrosis virus in farmed rainbow trout in North Macedonia. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-03 Aleksandar Cvetkovikj,Vladimir Radosavljevic,Argelia Cuenca,Biljana Strojmanovska,Jelena Maksimovic-Zoric,Iskra Cvetkovikj,Niels Jørgen Olesen
ABSTRACT: Infectious haematopoietic necrosis virus (IHNV) is the causative agent of infectious haematopoietic necrosis (IHN), a disease of salmonids responsible for great economic losses. The disease occurs in most parts of the world where rainbow trout is reared but has not been previously reported in North Macedonia. In this study, 150 pooled samples in total, each consisting of organ mix of 10 freshly
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Occurrence of Batrachochytrium dendrobatidis in Sweden: higher infection prevalence in southern species. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-09-03 Sara Meurling,Simon Kärvemo,Niki Chondrelli,Maria Cortazar Chinarro,David Åhlen,Lola Brookes,Per Nyström,Marika Stenberg,Trenton W J Garner,Jacob Höglund,Anssi Laurila
ABSTRACT: The chytrid fungus Batrachochytrium dendrobatidis (Bd) has caused worldwide declines in amphibian populations. While Bd is widespread in southern and central Europe, its occurrence and distribution in northernmost Europe is mostly unknown. We surveyed for Bd in breeding anurans in Sweden by sampling 1917 amphibians from 101 localities and 3 regions in Sweden (southern, northern and central)
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Enhancement of shrimp health and immunity with diets supplemented with combined probiotics: application to Vibrio parahaemolyticus infections. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-06-18 Chettupon Pooljun,Sunisa Daorueang,Wattana Weerachatyanukul,Sataporn Direkbusarakom,Pitchanee Jariyapong
ABSTRACT: The application of probiotics for disease control in aquaculture is now a convincing approach towards replacement of antibiotics, which can cause adverse effects in aquatic animals and humans. In this study, we combined 2 probiotics, Lactobacillus acidophilus and Saccharomyces cerevisiae, with shrimp feed to create 2 formulas (WU8 and WU9), which were fed for 10 d to juvenile shrimp Penaeus
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First report of bacterial kidney disease in coho salmon Oncorhynchus kisutch in Russia. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-06-18 N V Sergeenko,E A Ustimenko,M G Eliseikina,A D Kuhlevskiy,E V Bochkova,T V Ryazanova
ABSTRACT: This paper describes the first case of bacterial kidney disease (BKD) to be identified in coho salmon Oncorhynchus kisutch in Russia. The fish in question was caught in Lake Bolshoi Vilyui on the Kamchatka Peninsula. The diseased fish had foci of granulomatous inflammation in the kidneys. The diagnosis was confirmed by isolating the bacterium Renibacterium salmoninarum from kidney tissue
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Coadministration of citric acid allows oxytetracycline dose reduction in yellowtail Seriola quinqueradiata infected with Vibrio anguillarum. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-06-18 Kousuke Akiyama,Noritaka Hirazawa,Akimasa Hatanaka
ABSTRACT: Oxytetracycline (OTC) has been commonly used as an effective antibiotic against various fish bacterial diseases, including vibriosis. In this study, the absorption-enhancing effect of citric acid on oral OTC pharmacokinetics and treatment of artificial Vibrio anguillarum infection was evaluated in juvenile yellowtail Seriola quinqueradiata followed by serum OTC concentration analysis. When
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Livoneca redmanii (Isopoda, Cymothoidae) in meagre Argyrosomus regius: parasitological and molecular diagnosis and proposed control measure. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-06-18 Amr Fadel,Mohamed Bessat,Mohamed Abdel-Aziz
ABSTRACT: Isopodiosis in cultured meagre Argyrosomus regius was investigated at 3 farms in the northern lakes of Egypt throughout 2018, based upon prevalence rate, parasitological examination, and molecular identification by PCR targeting the large ribosomal subunit 16S of the rRNA gene. Further, the susceptibility of A. regius to isopod infection was experimentally evaluated under hyposalination of
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Isolation and maintenance of Batrachochytrium salamandrivorans cultures. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-06-18 Kristyn A Robinson,Kenzie E Pereira,Molly C Bletz,Edward Davis Carter,Matthew J Gray,Jonah Piovia-Scott,John M Romansic,Douglas C Woodhams,Lillian Fritz-Laylin
ABSTRACT: Discovered in 2013, the chytrid fungus Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, B. dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging
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Atlantic salmon Salmo salar and ballan wrasse Labrus bergylta display different susceptibility to clonal strains of Paramoeba perurans. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-07-02 O M V Dahle,S H Blindheim,A Nylund,E Karlsbakk,O Breck,H Glosvik,L Andersen
ABSTRACT: Amoebic gill disease (AGD), caused by the marine amoeba Paramoeba perurans, is an important disease of farmed Atlantic salmon Salmo salar L. in Norway. The use of wrasse as cleaner fish in salmon net pens raises questions about interspecies transmission of pathogens such as P. perurans. In this study, cohabitant transmission of clonal isolates of P. perurans between Atlantic salmon and ballan
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Atypical Aeromonas salmonicida vapA type V and Vibrio spp. are predominant bacteria recovered from ballan wrasse Labrus bergylta in Scotland. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-07-02 A Papadopoulou,T Wallis,J G Ramirez-Paredes,S J Monaghan,A Davie,H Migaud,A Adams
ABSTRACT: Healthy and/or moribund farmed and wild ballan wrasse Labrus bergylta (>0.5 to 900 g) were sampled from hatcheries (n = 2) and Atlantic salmon Salmo salar cage sites (n = 8) in Scotland between February 2016 and October 2018. Less than half of the sampled individuals (n = 43; 32.3%) had been vaccinated (autogenous polyvalent vaccine; dip and/or injection) against atypical furunculosis (type
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Prevalence of Batrachochytrium dendrobatidis in immature eastern hellbenders Cryptobranchus alleganiensis from North Carolina, USA. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-07-16 Lauren Diaz,Obed Hernandez-Gomez,Shem D Unger,Lori A Williams,Catherine M Bodinof Jachowski
ABSTRACT: Batrachochytrium dendrobatidis (Bd) has been detected in wild hellbender Cryptobranchus alleganiensis populations, with rare instances of chytridiomycosis and Bd-induced mortality. To date, Bd surveillance in hellbender populations has been disproportionately focused on adult age classes. A lingering question is whether Bd might contribute to hellbender declines through disproportionate negative
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Bonamia exitiosa in farmed native oysters Ostrea angasi in Australia: optimal epidemiological qPCR cut-point and clinical disease risk factors. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-08-06 T L Bradley,J A Mercer,J D Humphrey,N J G Moody,J C Hunnam
ABSTRACT: Bonamiosis has developed as a problem in Australian native oysters Ostrea angasi since the parasite Bonamia spp. was first detected in Port Phillip Bay, Victoria, in the early 1990s. At that time, large-scale mortalities in both farmed and wild oysters saw the demise of the pilot native oyster culture industry. More recent attempts to farm the species resulted in subclinical infections that
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Outbreak of cryptosporidiosis in African penguins Spheniscus demersus. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-08-06 Renata Hurtado,Nola J Parsons,Tertius A Gous,Stephen van der Spuy,Romy Klusener,Nicola Stander,Erna van Wilpe,Ralph E T Vanstreels
ABSTRACT: Cryptosporidium spp. are parasitic intracellular protozoa that infect the digestive, respiratory, and urinary tracts of vertebrates. The disease affects many different avian species across all continents, and >25 species and genotypes of Cryptosporidium have been documented infecting birds. We report on an outbreak of cryptosporidiosis in African penguin Spheniscus demersus chicks admitted
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Isolation and characterisation of an ISKNV-genotype megalocytivirus from imported angelfish Pterophyllum scalare. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-08-06 Yasuhiko Kawato,Peter G Mohr,Mark St J Crane,Lynette M Williams,Matthew J Neave,David M Cummins,Megan Dearnley,Sandra Crameri,Clare Holmes,John Hoad,Nicholas J G Moody
ABSTRACT: Using cultures of the SKF-9 cell line, megalocytivirus AFIV-16 was isolated from imported angelfish Pterophyllum scalare held in quarantine at the Australian border. The cytopathic effect caused by isolate AFIV-16 presented as cell rounding and enlargement, but complete destruction of the infected cell cultures did not occur. The infected cells demonstrated immunocytochemical reactivity with
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Simultaneous detection of scale drop disease virus and Flavobacterium columnare from diseased freshwater-reared barramundi Lates calcarifer. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-08-06 Pattarawit Kerddee,Ha Thanh Dong,Putita Chokmangmeepisarn,Channarong Rodkhum,Prapansak Srisapoome,Nontawith Areechon,Jorge Del-Pozo,Pattanapon Kayansamruaj
ABSTRACT: Freshwater farming of barramundi Lates calcarifer in Thailand is a growing sector in aquaculture, but mortalities due to infectious diseases are still a major threat to this industry. In 2018, an episode of severe mortality in juvenile barramundi was noted in a freshwater earth pond site. Fish presented with severe gill necrosis, as well as severe cutaneous hemorrhages, scale loss, and discoloration
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First detection of Francisella halioticida in mussels Mytilus spp. experiencing mortalities in France. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-08-20 Maud Charles,Antonio Villalba,Gary Meyer,Suzanne Trancart,Coralie Lagy,Ismaël Bernard,Maryline Houssin
ABSTRACT: This note describes the first detection of the bacteria Francisella halioticida in mussels Mytilus spp. from locations in Normandy and northern Brittany (France) experiencing high mussel mortalities, while it was not detected in the Bay of St Brieuc (northern Brittany), an area which was not affected by abnormal mussel mortality. The distribution of the bacteria in mussels seems to be restricted
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Complement proteins detected through iTRAQ-based proteomics analysis of serum from black carp Mylopharyngodon piceus in response to experimentally induced Aeromonas hydrophila infection. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-08-20 Wen Liu
ABSTRACT: The black carp Mylopharyngodon piceus is one of the culturally important ‘4 famous domestic fishes’ in China. Recently, infectious diseases caused by Aeromonas hydrophila have drastically altered the operation of the black carp farming industry. In the present study, isobaric tags for relative and absolute quantitation (iTRAQ) were combined with mass spectrometry analysis to screen for differentially
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Preliminary investigation of an idiopathic muscle disease in farmed burbot Lota lota. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-08-20 Constanze Pietsch,Fridolin Tschudi,Stefan Baumann,Heike Schmidt-Posthaus
ABSTRACT: The rearing of less established fish species in recirculating aquaculture systems (RASs) is increasing, but may require adaptations of the rearing facilities if health impairments occur. We observed several health issues in burbot Lota lota reared for up to 2 yr in a RAS and used microbiological, histological and molecular-biological methods to identify the causative agents. Minor skin trauma
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Bacteriological and histopathological analysis of Penaeus vannamei experimentally infected with Vibrio parahaemolyticus-AHPND strains. Dis. Aquat. Org. (IF 1.368) Pub Date : 2020-08-20 K G Aguilar-Rendón,R Lozano-Olvera,B Yáñez-Rivera,S A Soto-Rodriguez
ABSTRACT: Vibrio parahaemolyticus strains have been identified as the causative agent of acute hepatopancreas necrosis disease (Vp AHPND+) in shrimp. In the present work, a highly virulent (M0904) and less virulent (M0607) strain of Vp AHPND+ were used in experimental infections of Penaeus vannamei. Throughout the experiment, histopathological analyses were conducted on shrimp tissues, and samples