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Genetic Diversity in Natural Populations of the Near-Threatened Species Lignobrycon myersi (Characiformes, Triportheidae): Implications for Species Conservation. Zebrafish (IF 2.0) Pub Date : 2023-11-27 Lisianne de Souza Oliveira,Jamille de Araújo Bitencourt,José Henrique Galdino,Iracilda Sampaio,Paulo Luiz Souza Carneiro,Paulo Roberto Antunes de Mello Affonso
The river basins of Brazil contain a highly diverse ichthyofauna of remarkable endemism, including several threatened species. Accordingly, Lignobrycon myersi is a fish species distributed only in a few rivers from the state of Bahia, northeastern Brazil. Since this species is classified as Near Threatened and is poorly studied, efforts to understand the genetic structure of populations and putative
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An Affordable and Easy-to-Construct Zebrafish Housing System for Stable Long-Term Laboratory Research. Zebrafish (IF 2.0) Pub Date : 2023-11-22 Brian Lipscomb,Nicholas Seymour,Jada Lewis,Danny C LeBert
Zebrafish have become a go-to model organism for in vivo studies, in part because of their reputation as being inexpensive to rear and house. Multiple do-it-yourself designs are currently available that provide laboratories with cost-effective housing systems. Unfortunately, these designs suffer from a range of issues ranging from poor water cycling rates and fragile housing tanks to inconsistent water
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In Vitro Storage of Functional Sperm at Room Temperature in Zebrafish and Medaka. Zebrafish (IF 2.0) Pub Date : 2023-11-20 Kazumasa Takemoto,Toshiya Nishimura,Toshihiro Kawasaki,Yukiko Imai,Karine Levy,Neta Hart,Ivan Olaya,Sean M Burgess,Yaniv M Elkouby,Minoru Tanaka,Noriyoshi Sakai
The longevity of sperm in teleost such as zebrafish and medaka is short when isolated even in saline-balanced solution at a physiological temperature. In contrast, some internal fertilizers exhibit the long-term storage of sperm, >10 months, in the female reproductive tract. This evidence implies that sperm in teleost possesses the ability to survive for a long time under suitable conditions; however
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U2 and U4 snDNA Comparative Chromosomal Mapping in the Neotropical Fish Genera Apareiodon and Parodon (Characiformes: Parodontidae). Zebrafish (IF 2.0) Pub Date : 2023-10-05 Matheus Azambuja,Viviane Nogaroto,Orlando Moreira-Filho,Marcelo Ricardo Vicari
Small nuclear DNA (snDNA) are valuable cytogenetic markers for comparative studies in chromosome evolution because different distribution patterns were found among species. Parodontidae, a Neotropical fish family, is known to have female heterogametic sex chromosome systems in some species. The U2 and U4 snDNA sites have been found to be involved in Z and W chromosome differentiation in Apareiodon
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Functioning and Gene Expression of Adenosine A1 Receptor During Zebrafish (Danio rerio) Development. Zebrafish (IF 2.0) Pub Date : 2023-10-01 Giovanna Trevisan Couto,Higor Arruda Caetano,Guilherme Pietro da Silva,Liliana Rockenbach,Jéssica Scheid da Silva,Monica Ryff Vianna,Rosane Souza Da Silva
The A1 adenosine receptor is the most widely expressed P1 receptor in vertebrates, performing inhibitory tone of the nervous system. Increased levels of adenosine are crucial to promote tissue protection in threatening situations, such as convulsion and hypoxia. Zebrafish is an established model organism for studies on health and disease. In this study, we evaluated the functionality of A1 adenosine
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Incorporating Primer Amplification Efficiencies in Quantitative Reverse Transcription Polymerase Chain Reaction Experiments; Considerations for Differential Gene Expression Analyses in Zebrafish. Zebrafish (IF 2.0) Pub Date : 2023-09-19 Gillian Davis,Brianna Hameister,Cora Dunnum,Emily Vanderpas,Brad Carter
Quantitative reverse transcription polymerase chain reaction (RT-qPCR) is commonly used to measure the mRNA expression of target genes in zebrafish. Gene expression values from RT-qPCR are typically reported as relative fold-changes, and relative quantification of RT-qPCR data incorporates primer amplification efficiency values for each target gene. We describe the influence of the primer amplification
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Curcumin-Encapsulated Nanomicelles Promote Tissue Regeneration in Zebrafish Eleutheroembryo. Zebrafish (IF 2.0) Pub Date : 2023-08-29 Sai Sandhya Narra,Laura Gence,Latufa Youssouf,Joël Couprie,Pierre Giraud,Nicolas Diotel,Christian Lefebvre D'Hellencourt
Regenerative medicine is an emerging field of research aiming to understand the wound healing mechanisms and to develop new therapeutic strategies. Nanocarriers are used to improve drug bioavailability, solubility, and therapeutic abilities. In this study, we used for the first time curcumin loaded oligo kappa-carrageenan-graft-polycaprolactone (oligoKC-g-PCL) nanomicelles to investigate their regenerative
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Novel Development of Magnetic Resonance Imaging to Quantify the Structural Anatomic Growth of Diverse Organs in Adult and Mutant Zebrafish. Zebrafish (IF 2.0) Pub Date : 2023-08-21 Sonal Sharma,Sergey Magnitsky,Emily Reesey,Mitchell Schwartz,Suraiya Haroon,Manuela Lavorato,Sherine Chan,Rui Xiao,Benjamin J Wilkins,Daniel Martinez,Christoph Seiler,Marni J Falk
Zebrafish (Danio rerio) is a widely used vertebrate animal for modeling genetic diseases by targeted editing strategies followed by gross phenotypic and biomarker characterization. While larval transparency permits microscopic detection of anatomical defects, histological adult screening for organ-level defects remains invasive, tedious, inefficient, and subject to technical artifact. Here, we describe
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Lack of Stim2 Affects Vision-Dependent Behavior and Sensitivity to Hypoxia. Zebrafish (IF 2.0) Pub Date : 2023-08-01 Iga Wasilewska,Łukasz Majewski,Dobrochna Adamek-Urbańska,Shamba S Mondal,Sofiia Baranykova,Rishikesh K Gupta,Dominik Bielecki,Cecilia L Winata,Jacek Kuznicki
Stromal interaction molecules (STIMs) are endoplasmic reticulum-resident proteins that regulate Ca2+ homeostasis and signaling by store-operated calcium entry (SOCE). The different properties and functions of STIM1 and STIM2 have been described mostly based on work in vitro. STIM2 knockout mice do not survive until adulthood. Therefore, we generated and characterized stim2a and stim2b double-knockout
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Modeling of Zebrafish Housing Temperatures During Power Outage and Instrument Failure. Zebrafish (IF 2.0) Pub Date : 2023-08-01 Ilkka Paatero
Power outages can happen anywhere and anytime for various reasons. This threat affects also scientific work of biologists. Especially problematic area is aquatic animal husbandry, where life support of the animals is dependent on continuous electricity supply and years of scientific work may depend on the well-being of these animal stocks. Therefore, tools to estimate and control these risks are needed
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Zebrafish (Danio rerio) Gynogenetic Production by Heat Shock: Comparison Between Mitotic and Meiotic Treatment. Zebrafish (IF 2.0) Pub Date : 2023-08-01 Príncia Grejo Setti,Ricardo Carneiro Borra,Francisco de Menezes Cavalcante Sassi,Marcelo de Bello Cioffi,Hirla Costa Silva Fukushima
Inbred species are useful resources for a variety of biomedical research applications. To create isogenic zebrafish, it is feasible to stop meiosis II (repeatedly) or mitosis (two times) in a haploid embryo by applying pressure or by delivering a heat shock, respectively. In this study, to improve the repeatability, we suggest a less complicated approach based on sperm ultraviolet-C (UV-C) exposure
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Fish in a Dish: Using Zebrafish in Authentic Science Research Experiences for Under-represented High School Students from West Virginia. Zebrafish (IF 2.0) Pub Date : 2023-07-14 Rebecca A Coltogirone,Summer L Kuhn,Sean P Freeland,Sadie A Bergeron
Early research experiences positively affect students' interest in STEM careers, and develop practical science and critical thinking skills. However, outreach opportunities are not equally accessible for all students. In states like West Virginia, where many students live in rural Appalachian communities, opportunities for engaging in STEM experiences are limited. In addition, rural teachers may not
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Manganese Exposure Induces Cellular Aggregates and the Accumulation of β-Catenin in Skin of Zebrafish Embryos. Zebrafish (IF 2.0) Pub Date : 2023-07-05 Geyse Gomes,José Leonardo Oliveira,Manoel Luis Costa,Claudia Mermelstein,Natália Martins Feitosa
The effects of manganese (Mn) toxicity in different organs and tissues in humans and other vertebrates have been studied since the beginning of the past century, but most of its cellular effects remain largely unknown. In this study, we studied the effects of Mn in zebrafish, at the cellular level, due to the transparent nature of zebrafish larvae that enables a powerful analysis under the light microscope
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Influence of Methylene Blue or Dimethyl Sulfoxide on Larval Zebrafish Development and Behavior. Zebrafish (IF 2.0) Pub Date : 2023-07-04 Joan M Hedge,Deborah L Hunter,Erik Sanders,Kimberly A Jarema,Jeanene K Olin,Katy N Britton,Morgan Lowery,Bridget R Knapp,Stephanie Padilla,Bridgett N Hill
The use of larval zebrafish developmental testing and assessment, specifically larval zebrafish locomotor activity, has been recognized as a higher throughput testing strategy to identify developmentally toxic and neurotoxic chemicals. There are, however, no standardized protocols for this type of assay, which could result in confounding variables being overlooked. Two chemicals commonly employed during
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Method for Visualization of Emergency Granulopoiesis in the Zebrafish Embryo. Zebrafish (IF 2.0) Pub Date : 2023-06-12 Ramizah Syahirah,Jennifer Beckman,Hanna Malik,Alan Y Hsu,Qing Deng
Emergency granulopoiesis (EG) is a response to severe inflammation in which increased neutrophils are generated in the hematopoietic tissue. Photolabeling is utilized to distinguish newly developed neutrophils from existing neutrophils. However, this technique requires a strong laser line and labels subsets of the existing neutrophils. Here we create a transgenic zebrafish line that expresses a time-dependent
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Fish Hacks: Hybridizing Stand-Alone Zebrafish System Plumbing and Pumps to Extend and Improve Function. Zebrafish (IF 2.0) Pub Date : 2023-06-01 Jacob Starkey,Eric J Horstick
One of the greatest expenses in running a zebrafish laboratory is the aquatic systems used for housing. These critical pieces of equipment are essential and incorporate components undergoing constant activity in pumping water, monitoring, dosing, and filtration. The systems available on the market are robust, yet ongoing activity eventually leads to the need for repair or replacement. Moreover, some
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An Efficient and Reproducible Method for the Isolation and Culture of Primary Cardiomyocytes from Adult Zebrafish. Zebrafish (IF 2.0) Pub Date : 2023-06-01 Chunyan Zhang,Yanyi Sun,Zhenyue Chen
Zebrafish is a popular animal model in regeneration studies due to their ability to regenerate the heart. Primary cardiomyocytes could be an alternative tool for studying the intrinsic mechanisms of cardiovascular disease in vitro. Thus, our objective is to develop an efficient protocol to isolate primary cardiomyocytes from zebrafish hearts. Low concentration of digestive enzyme (0.5 mg/mL collagenase
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Ronidazole Is a Superior Prodrug to Metronidazole for Nitroreductase-Mediated Hepatocytes Ablation in Zebrafish Larvae. Zebrafish (IF 2.0) Pub Date : 2023-05-25 Yuhang Chen,Peipei Li,Ting Chen,Hanjie Liu,Peijian Wang,Xiaozhen Dai,Qingliang Zou
The liver plays a very important role in physiological processes of the human body. Liver regeneration has developed into an important area of study in liver disease. The Mtz (metronidazole)/NTR (nitroreductase)-mediated cell ablation system has been widely used to study the processes and mechanisms of liver injury and regeneration. However, high concentrations and toxic side effects of Mtz severely
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An Outbreak of the Nematode Parasite Eustrongylides spp. (Nematoda: Dioctophymatidae) in a Zebrafish (Danio rerio) Facility. Zebrafish (IF 2.0) Pub Date : 2023-04-18 Maria Alice Fusco,Vanessa de Sousa Rizzo-Valente,Vinicius Figueiredo Vizzoni,Ricardo Silva de Miranda,Caroline Corrêa de Aguiar,Roberta da Costa Escaleira
Despite advances in research employing zebrafish (Danio rerio) as an animal model, it is still difficult to obtain animals with attested sanitary qualities from commercial breeders. This study reports the first-time occurrence of Eustrongylides spp. parasitism in a zebrafish colony recently acquired from a pet store supplier to begin a research colony at a scientific facility. This parasite has not
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Phase Retrieval-Based Phase-Contrast Imaging and CT of Living Zebrafish. Zebrafish (IF 2.0) Pub Date : 2023-04-06 Rongbiao Tang,Yi Chen,Fuhua Yan,Ke-Min Chen
Zebrafish are widely used as experimental animal models. They are small and move fast in the water. Real-time imaging of fast-moving zebrafish is a challenge, and it requires that the imaging technique has higher spatiotemporal resolution and penetration ability. The purpose of this study was to evaluate the feasibility of dynamic phase retrieval (PR)-based phase-contrast imaging (PCI) for real-time
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Three-Dimensional Structure of Inner Ear Hair Cell Ribbon Synapses in a Zebrafish Model of Usher Syndrome Type 1B. Zebrafish (IF 2.0) Pub Date : 2023-04-01 Kenneth C Riley,Alaa Koleilat,Joseph A Dugdale,Shawna A Cooper,Trace A Christensen,Lisa A Schimmenti
Our understanding of inner ear hair cell ultrastructure has heretofore relied upon two-dimensional imaging; however, serial block-face scanning electron microscopy (SBFSEM) changes this paradigm allowing for three-dimensional evaluation. We compared inner ear hair cells of the apical cristae in myo7aa-/- null zebrafish, a model of human Usher Syndrome type 1B, to hair cells in wild-type zebrafish by
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Tetrahydroxystilbene Glucoside Attenuates Oxidative Stress-Induced Aging by Regulating Oxidation Resistance and Inflammation in Larval Zebrafish. Zebrafish (IF 2.0) Pub Date : 2023-04-01 Hui Xia,Xue Cheng,Mengxi Cao,Xiongjie Sun,Fuyi He,Xiaowei Yao,Hongtao Liu
Population aging is a global problem worldwide, and the discovery of antiaging drugs and knowledge of their potential molecular mechanisms are research hotspots in biomedical field. Tetrahydroxystilbene glucoside (TSG) is a natural component isolated from Heshouwu (Polygonum multiflorum Thunb.). It has been widely used to treat various chronic diseases for its remarkable biological activities. In this
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Lethal and Sublethal Toxicity of Un-Ionized Ammonia to Early-Life Stages of Danio rerio. Zebrafish (IF 2.0) Pub Date : 2023-03-23 Célio Freire Mariz,Maria Karolaine de Melo Alves,Shaieny Marcela Ventura Dos Santos,Romulo Nepomuceno Alves,Paulo S M Carvalho
Un-ionized ammonia (NH3) is a prevalent contaminant found in aquatic ecosystems, frequently associated with complex mixtures of other toxic contaminants. Early embryo-larval stages of zebrafish Danio rerio became an important model for water quality monitoring, and it is necessary to characterize its susceptibility to NH3 exposure. Fertilized eggs were exposed to NH3 concentrations ranging from 0.02
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Cytogenetic and Molecular Characterization of Eigenmannia aff. desantanai (Gymnotiformes: Sternopygidae): A First Report of System of Sex Chromosomes ZW1W2/ZZ in Gymnotiformes. Zebrafish (IF 2.0) Pub Date : 2023-03-16 Laís de Araújo,Laura Ivana Ramos,Margarida Maria de Rossi Vieira,Alessandra Valéria de Oliveira,Ana Luiza de Brito Portela-Castro,Luciana Andreia Borin-Carvalho,Carlos Alexandre Fernandes
Gymnotiformes a monophyletic group of fish endemic to the Neotropics, represent an important component of the freshwater ichthyofauna that presents relevant taxonomic problems. Thus, in view of the morphological complexity involving Eigenmannia (Gymnotiformes) fish species, this study aimed to characterize Eigenmannia aff. desantanai of the upper Paraguay River basin through cytogenetic and molecular
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Chromosomal Mapping of the Histone Multigene Family and U2 snRNA in Hypancistrus Species (Siluriformes, Loricariidae) from the Brazilian Amazon. Zebrafish (IF 2.0) Pub Date : 2023-02-01 Carlos Eduardo Vasconcelos Dos Santos,Bruno Rafael Ribeiro de Almeida,Flávia Dos Santos Tavares,Luan Felipe da Silva Frade,Adauto Lima Cardoso,André Luiz Alves de Sá,Cleusa Yoshiko Nagamachi,Leandro Melo de Sousa,Julio Cesar Pieczarka,Renata Coelho Rodrigues Noronha
Loricariidae (Siluriformes) comprises ∼1026 species of neotropical fish, being considered the most diverse among the Siluriformes. Studies on repetitive DNA sequences have provided important data on the evolution of the genomes of members of this family, especially of the Hypostominae subfamily. In this study, the chromosomal mapping of the histone multigene family and U2 snRNA was performed in two
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Zebrafish sox2 Is Required for the Swim Bladder Inflation by Controlling the Swim-Up Behavior. Zebrafish (IF 2.0) Pub Date : 2023-02-01 Shasha Cao,Zhangji Dong,Xiaohua Dong,Wenshuang Jia,Fuyou Zhou,Qingshun Zhao
The swim bladder functions to maintain the fish balance at a certain position under water. Although the motoneuron-dependent swim-up behavior is important for swim bladder inflation, the underlying molecular mechanism remains largely unknown. We generated a sox2 KO zebrafish using TALEN and found that the posterior chamber of the swim bladder was uninflated. The tail flick and the swim-up behavior
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wnt11f2 Zebrafish, an Animal Model for Development and New Insights in Bone Formation. Zebrafish (IF 2.0) Pub Date : 2023-02-01 Caroline Caetano da Silva,Agnes Ostertag,Ratish Raman,Marc Muller,Martine Cohen-Solal,Corinne Collet
Wnt signaling is a key regulator of osteoblast differentiation and mineralization in humans and animals, mediated by the canonical Wnt/β-catenin and noncanonical signaling pathways. Both pathways are crucial in regulating osteoblastogenesis and bone formation. The zebrafish silberblick (slb) carries a mutation in wnt11f2, a gene that contributes to embryonic morphogenesis; however, its role in bone
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Molecular Markers Reveal a New and Possibly Threatened Species of Cnesterodon (Poeciliidae, Cnesterodontini) from the Upper Paraná River Basin, Brazil Zebrafish (IF 2.0) Pub Date : 2023-01-25 João Paulo Morais-Silva, Bárbara Scorsim, Geovana Gonçalves, Augusto Frota, Weferson Júnio da Graça, Alessandra Valéria de Oliveira
Cnesterodon comprises 10 valid species occurring in the major river basins of South America. Recent ichthyofaunistic studies in the Ivaí River basin, upper Paraná River system, suggested the existence of a possible new species, which was identified as Cnesterodon sp. based on morphological characters. Currently, the use of molecular tools has proved to be fundamental in aiding phylogenetics and cataloging
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Metoclopramide as a Potential Antipsychotic Against Long-Term Methionine Exposure in Zebrafish Zebrafish (IF 2.0) Pub Date : 2022-12-27 Hemen Ved, Gaurav Doshi, Nirav Bhatia, Pravin Kale
Methionine (MET) contributes to brain function and is required for proper functioning of the central nervous system. However, exceptionally high levels of MET and its metabolites in plasma have been found to be toxic and can lead to cell alterations. Long-term exposure to MET has been shown to mimic psychotic symptoms in schizophrenic patients and rodents. The present study evaluated behavioral and
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Automated Zebrafish Phenotype Pattern Recognition: 6 Years Ago, and Now Zebrafish (IF 2.0) Pub Date : 2022-12-16 Mark Schutera, Luca Rettenberger, Markus Reischl
The article assesses the developments in automated phenotype pattern recognition: Potential spikes in classification performance, even when facing the common small-scale biomedical data set, and as a reader, you will find out about changes in the development effort and complexity for researchers and practitioners. After reading, you will be aware of the benefits and unreasonable effectiveness and ease
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Common Aquarium Plants as an Enrichment Strategy in Zebrafish Facilities Zebrafish (IF 2.0) Pub Date : 2022-12-16 Benjamin Tsang, Robert T. Gerlai
An increasing number of laboratories utilize zebrafish as this species is now represented in practically every subfield of biology research. Environmental enrichment has been shown to improve welfare and health of a large number of species of animals studied in laboratories, kept in zoos, or used in agriculture. However, most laboratories keep zebrafish in barren tanks. Artificial and live aquatic
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Comparative Analysis of Central Corneal Thickness in Four Fish Models Zebrafish (IF 2.0) Pub Date : 2022-12-16 Jinpeng Li, Juan Du, Aiping Deng, Ziyang Chen, Yusong Guo, Zhongduo Wang
To understand the left–right asymmetry of vertebrate eyes, this study measured the central corneal thickness (CCT) of Oryzias curvinotus, Oryzias melastigma, Oryzias latipes, and zebrafish with optical coherence tomography. The results showed that the CCTs were significant differences among different species and groups, even between the right and left eyes of each fish. The values of the CCTs (mean ± SD
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The Effects of Plant Protein-Enriched Live Food on Larval Zebrafish Growth and the Status of Its Digestive Tract Development Zebrafish (IF 2.0) Pub Date : 2022-12-16 Connor W. Schwepe, Michal Wojno, Giovanni S. Molinari, Karolina Kwasek
Live food is necessary for the proper development of zebrafish larvae, providing nutrition in a form that is easily digestible and available to the larvae. Live food is commonly enriched to increase the dietary content of certain nutrients. However, little research has been done on protein-based enrichments, especially those of plant origin. This study sought to examine how different quality protein
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A Simple Method for Inducing Masculinization of Zebrafish Stocks Using 17α-Methyltestosterone Zebrafish (IF 2.0) Pub Date : 2022-12-16 Jennifer L. Matthews, Joy Murphy, Andrzej Nasiadka, Zoltan M. Varga
Severely skewed sex ratios in zebrafish stocks can pose significant hurdles for line propagation and sperm cryopreservation. To overcome female-biased sex ratios in stocks derived from imported sperm samples, the Zebrafish International Resource Center has implemented routine supplementation of larval food with 17α-methyltestosterone to skew gonadal sex differentiation toward masculinization. Resulting
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Call for Special Issue Papers: 2023 Education Issue of Zebrafish. Zebrafish (IF 2.0) Pub Date : 2022-12-01 Lara Hutson,Jennifer Liang,Michael Pickart,Christopher Pierret,Tamara Franz-Odendaal,Madelyn Jackstadt
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A Simple Blood Vessel Imaging Protocol for Live Zebrafish Larva Zebrafish (IF 2.0) Pub Date : 2022-10-17 S. Vinoth, Velanganni Selvaraj, Hemagowri Venkatasubramanian, Kirankumar Santhakumar
Zebrafish larva is a simple model system to study blood vessel development because of its excellent optical properties. Current methods to study blood vessels in zebrafish involve utilizing either blood vessel-specific transgenic lines or specialized techniques such as microangiography and video capillaroscopy. In this study, we have developed a simple protocol using ImageJ to observe the blood vessels
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Evaluation of Media Conductivity and a Combination of Iodine and Sodium Hypochlorite Surface Disinfection on Zebrafish (Danio rerio) Embryo Viability and Morphology Zebrafish (IF 2.0) Pub Date : 2022-10-17 Gerardo Mendoza Jr., Adedeji A. Afolalu, Kvin Lertpiriyapong, Neil S. Lipman, Christine Lieggi
Embryo surface disinfection in either an iodine or sodium hypochlorite (NaOCl) solution is commonly performed on imported zebrafish embryos to decrease pathogen introduction into a facility. The impact of the consecutive use of these disinfectants and the conductivity of the culture media on embryo survival and development post-disinfection have not been evaluated. Iodine (12.5–25 ppm) is effective
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Comparative Cytogenetic and Sequence Analysis of U Small Nuclear RNA Genes in Three Ancistrus Species (Siluriformes: Loricariidae) Zebrafish (IF 2.0) Pub Date : 2022-10-17 Stephane Carolenn Quadros Schott, Larissa Glugoski, Matheus Azambuja, Orlando Moreira-Filho, Marcelo Ricardo Vicari, Viviane Nogaroto
Ancistrus presents a wide karyotypic diversity, resulting from numeric and structural chromosomal rearrangements. It has been proposed that some genome-specific regions containing repetitive units could organize prone-to-break DNA sites in Loricariidae, triggering chromosomal rearrangements such as Robertsonian fusions (Rb fusions), centric fissions, translocations, and inversions. The tandemly repeats
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Use of Zebrafish as a Model Organism to Study Oxidative Stress: A Review Zebrafish (IF 2.0) Pub Date : 2022-10-17 Sabarna Chowdhury, Surjya Kumar Saikia
Dioxygen is an integral part of every living organism, but its concentration varies from organ to organ. Production of metabolites from dioxygen may result in oxidative stress. Since oxidative stress has the potential to damage various biomolecules in the cell, therefore, it has presently become an active field of research. Oxidative stress has been studied in a wide range of model organisms from vertebrates
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Assessment of Various Standard Fish Diets on Growth and Fecundity of Platyfish (Xiphophorus maculatus) and Medaka (Oryzias latipes) Zebrafish (IF 2.0) Pub Date : 2022-10-17 Crystal Russo, Merritt Drewery, Carolyn T. Chang, Markita Savage, Lindsey Sanchez, Zoltan Varga, Michael L. Kent, Ronald Walter, Yuan Lu
Several small freshwater fish species are utilized as models for human conditions and disease in biomedical research. Research animal diets are generally tailored to optimize growth, fecundity, and produce healthy research animals. However, a lack of reference diets presents a barrier in comparative studies between aquatic animal models and even among laboratories using the same species. Therefore
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The Need for a Framework Addressing the Temporal Aspects of Fish Sperm Motility Leading to Community-Level Standardization Zebrafish (IF 2.0) Pub Date : 2022-08-11 Harvey Blackburn, Leticia Torres, Yue Liu, Terrence R. Tiersch
Motility is a widely available parameter that can be used to assess sperm quality of aquatic species. Sperm from fishes with external fertilization usually undergo a dynamic and short-lived period of motility after activation. The common practice of assigning a single value at an arbitrary peak of motility presents challenges for reproducibility, community-level standardization, and comparisons across
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Telomerase Activity in Androgenetic Rainbow Trout with Growth Deficiency and in Normally Developed Individuals Zebrafish (IF 2.0) Pub Date : 2022-08-11 Ligia Panasiak, Marcin Kuciński, Agata Błaszczyk, Konrad Ocalewicz
Role of telomerase in specimens with retarded growth (dwarfs) has not been thoroughly examined to date. Considering that some of the fish species show correlation between somatic growth and activity of telomerase, it has been tempting to assume that pattern of telomerase activity in specimens with retarded growth and these with normal growth rate may vary. In the present research, telomerase activity
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Dopamine Receptors Gene Expression Pattern and Locomotor Improvement Differ Between Female and Male Zebrafish During Spinal Cord Auto Repair Zebrafish (IF 2.0) Pub Date : 2022-08-11 Parastoo Hosseini, Sara Mirsadeghi, Saeid Rahmani, Amin Izadi, Mohammad Rezaei, Zahra Ghodsi, Vafa Rahimi-Movaghar, Sahar Kiani
The dopaminergic system, a spinal cord (SC) motor circuit regulator, is administrated by sexual hormones and evolutionary conserved in all vertebrates. Accordingly, we hypothesized that the dopamine receptor (DAR) expression pattern may be dissimilar in female and male zebrafish SC auto repair. We implemented an uncomplicated method to induce spinal cord injury (SCI) on fully reproductive adult zebrafish
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Anesthesia Overdose Versus Rapid Cooling for Euthanasia of Adult Zebrafish Zebrafish (IF 2.0) Pub Date : 2022-08-11 Jorge M. Ferreira, Luís Félix, Sara Jorge, Sandra M. Monteiro, I. Anna S. Olsson, Ana M. Valentim
The rapid increase in zebrafish use needs to be accompanied by research into the refinement of procedures. The European (EU) Directive lists three possible euthanasia methods for fish: anesthetic overdose, electrical stunning, and concussion. However, for small fish such as zebrafish, concussion and electrical stunning are difficult to perform, leaving anesthetic overdose as the most used method. Our
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A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish Zebrafish (IF 2.0) Pub Date : 2022-08-11 Ji Yun Jang, Ting Liang, Myeong-Kyu Kim, Kyung Wook Kang, Bora Lee, Seok-Yong Choi
Genotyping usually entails analysis of the products of polymerase chain reaction (PCR) carried out with genomic DNA (gDNA) as template, and is employed for validation of mutant or transgenic organisms. For genotyping of adult zebrafish, gDNA is often extracted from clipped caudal fin or skin mucus through either alkaline lysis using NaOH or proteinase K (PK) treatment. Further purification of the gDNA
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Dopamine Level Affects Social Interaction and Color Preference Possibly Through Intestinal Microbiota in Zebrafish Zebrafish (IF 2.0) Pub Date : 2022-06-10 Ju Wang, Feng Zheng, Lifen Yin, Shengnan Shi, Bing Hu, Hao Qu, Lei Zheng
Accumulating researches suggest that the microbiota reside in the gastrointestinal system can influence neurodevelopment of brain and programming of behaviors. However, the mechanism underlining the relationship between shoals' behaviors and intestinal microbiota remain controversial and the roles of responsible neurotransmitters are still unclear. Here we show that shoaling behavior affected the color
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Identification of Transcription Factor Binding Sites by Cleavage Under Target and Release Using Nuclease in Zebrafish Zebrafish (IF 2.0) Pub Date : 2022-06-10 Radwa Barakat, Clyde A. Campbell, Raquel Espin-Palazon
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) has emerged as a chromatin profiling strategy that excels traditional methods. Although CUT&RUN has been widely utilized in mammalian cells, its use in the zebrafish is at its early stages. In this study, we have developed a protocol to successfully perform CUT&RUN to map transcription factor (TF) binding sites in embryonic, adult tissues
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Simple and High-Throughput Rheotaxis Behavioral Assay for Zebrafish Larva Zebrafish (IF 2.0) Pub Date : 2022-06-10 M.S. Ananthakrishna Tantry, V.S. Harini, Kirankumar Santhakumar
Zebrafish (Danio rerio) is used as a model for studying sensorineural hearing loss. The damage to the hair cells can be assessed by scoring rheotaxis behavior in zebrafish. In this study, we newly designed a rheotaxis behavioral assay protocol capable of quantifying rheotaxis behavior in zebrafish larvae. We chemically induced ototoxicity in the larvae using copper sulfate, a well-known ototoxin, and
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A New Transgenic Line for Rapid and Complete Neutrophil Ablation Zebrafish (IF 2.0) Pub Date : 2022-06-10 Christopher J. Hall, Jonathan W. Astin, Jeff S. Mumm, David F. Ackerley
Zebrafish lines expressing nitroreductase (NTR) in specific cell compartments, which sensitizes those cells to metronidazole (MTZ)-mediated ablation, have proven extremely useful for studying tissue regeneration and investigating cell function. In contrast to many cells, neutrophils are comparatively resistant to the NTR/MTZ targeted ablation strategy. Recently, a rationally engineered variant of NTR
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Feeding Rate and Protein Quality Differentially Affect Growth and Feeding Efficiency Response Variables of Zebrafish (Danio rerio) Zebrafish (IF 2.0) Pub Date : 2022-06-10 Shaley Valentine, Karolina Kwasek
Manipulating feeding rate and protein quality may improve growth and feeding efficiency of cultured species. However, whether feeding rate, protein quality, or their interaction has a greater effect on growth and feeding efficiency response variables is unknown. To determine whether feeding rate and protein quality individually or interactively affect growth and feeding efficiency, juvenile Zebrafish
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The Sound World of Zebrafish: A Critical Review of Hearing Assessment Zebrafish (IF 2.0) Pub Date : 2022-04-19 Arthur N. Popper, Joseph A. Sisneros
Zebrafish, like all fish species, use sound to learn about their environment. Thus, human-generated (anthropogenic) sound added to the environment has the potential to disrupt the detection of biologically relevant sounds, alter behavior, impact fitness, and produce stress and other effects that can alter the well-being of animals. This review considers the bioacoustics of zebrafish in the laboratory
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TP53 Mutant Versus Wild-Type Zebrafish Larvae Under Starvation Stress: Larvae Can Live Up to 17 Days Post-Fertilization Without Food Zebrafish (IF 2.0) Pub Date : 2022-04-19 Konstantinos Voskarides, Constantina Koutsofti, Maria Pozova
In this study, an experimental protocol has been developed for comparing survival rates of mutant and wild-type zebrafish larvae under extreme starvation. Zebrafish larvae were placed in 96-well plates at fourth day postfertilization (dpf) and larvae were not fed at all from hatching to cease. Zdf1 zebrafish line was used, a strain carrying the (cancer) pathogenic TP53-M214K amino acid substitution
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Design and Husbandry Considerations for a Containment Level 2 Aquatic Facility Zebrafish (IF 2.0) Pub Date : 2022-04-19 Nicola Goodwin, Lynda Westall
The greatly increased use of aquatic species to study disease over the past 20 years necessitates understanding their husbandry and housing requirements to optimize research and welfare and to ensure compliance with regulations. To achieve these goals, aquatic systems have expanded from pet shop and home aquaria to research-grade systems incorporating designs and features to increase their robustness
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Dedicated Chamber for Multimodal In Vivo Imaging of Adult Zebrafish Zebrafish (IF 2.0) Pub Date : 2022-04-19 Steven Seeger, Milan Zvolský, Salim Melikov, Maja Frerkes, Magdalena Rafecas
In vivo imaging of adult zebrafish is challenging, particularly for dynamic or long acquisitions when using, for example, positron emission tomography, single photon emission computed tomography, computed tomography (CT), or magnetic resonance imaging (MRI). An aqueous environment is indispensable to ensure animal welfare, but commercial small-animal imaging chambers do not provide such conditions
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Cytogenetic and Molecular Characterization of Oligosarcus pintoi (Characidae): A New Record of Supernumerary Chromosome in this Species Zebrafish (IF 2.0) Pub Date : 2022-04-19 Isabelle Pereira Mari-Ribeiro, Bárbara Scorsim, Alessandra Valéria de Oliveira, Ana Luiza de Brito Portela-Castro
The genus Oligosarcus currently comprises 24 valid species distributed in the major river basins of South America. In this group, nine species were cytogenetically investigated, and found to share a diploid number of 50 chromosomes. Despite the conservation of the diploid number, variations in the karyotypic formula, number and position of the nucleolar organizer regions, and longitudinal bands have
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Purification and Identification of the 20S Proteasome Complex from Zebrafish Zebrafish (IF 2.0) Pub Date : 2022-02-14 Ali Md. Hasan, Md. Maisum Sarwar Jyoti, Md. Rubel Rana, Md. Rezanujjaman, Toshinobu Tokumoto
The proteasome is a large polymeric protease complex responsible for degradation of intracellular proteins and generation of peptides. In this study, we purified a native 20S proteasome protein complex from zebrafish (Danio rerio) from the whole body. The cytosolic fraction of zebrafish hydrolyzed Suc-Leu-Leu-Val-Tyr-MCA (Suc-LLVY-MCA), a well-known substrate for the proteasome, in the presence of
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Evolutionary Dynamics of Two Classes of Repetitive DNA in the Genomes of Two Species of Elopiformes (Teleostei, Elopomorpha) Zebrafish (IF 2.0) Pub Date : 2022-02-14 Rodrigo Petry Corrêa de Sousa, Carolina Pinheiro Vasconcelos, Nayara Furtado do Rosário, Aldemir Branco de Oliveira-Filho, Edivaldo Herculano Corrêa de Oliveira, Marcelo de Bello Cioffi, Marcelo Vallinoto, Gláucia Caroline Silva-Oliveira
The order Elopiformes includes fish species of medium to large size with a circumglobal distribution, in both the open sea, coastal, and estuarine waters. The Elopiformes are considered an excellent model for evolutionary studies due to their ample adaptive capacity, which allow them to exploit a range of different ecological niches. In this study, we analyzed the karyotype structure and distribution
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Ontogenetic Changes in Blood Osmolality During the Postembryonic Development of Zebrafish (Danio rerio) Zebrafish (IF 2.0) Pub Date : 2022-02-14 Guy Charmantier, Mai Nguyen-Chi, Georges Lutfalla
The zebrafish Danio rerio is a teleost model species widely used in developmental genetics, biomedical studies, toxicology, and drug screening. Despite the interest of this species in research, little is known through indirect observations about its blood osmolality, which is a key parameter for diverse experiments. In this study, we directly measured blood osmolality using nano-osmometry at different
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Zebrafish Heme Oxygenase 1a Is Necessary for Normal Development and Macrophage Migration Zebrafish (IF 2.0) Pub Date : 2022-02-14 Kaiming Luo, Masahito Ogawa, Anita Ayer, Warwick J. Britton, Roland Stocker, Kazu Kikuchi, Stefan H. Oehlers
Heme oxygenase function is highly conserved between vertebrates where it plays important roles in normal embryonic development and controls oxidative stress. Expression of the zebrafish heme oxygenase 1 genes is known to be responsive to oxidative stress suggesting a conserved physiological function. In this study, we generate a knockout allele of zebrafish hmox1a and characterize the effects of hmox1a
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Accelerating Chemobehavioral Phenotypic Screening in Neurotoxicology Using a Living Embryo Array System Zebrafish (IF 2.0) Pub Date : 2022-02-14 Jason Henry, Yutao Bai, Florian Kreuder, David Mawdsley, Jan Kaslin, Donald Wlodkowic
Large-scale chemobehavioral phenotyping with zebrafish embryos is a promising avenue for accelerated neurotoxicity testing and discovery of behavior-modifying neuroceuticals. These strategies are hampered by lack of effective embryo in-test positioning, wide-field imaging, and high-throughput bioinformatic analytics. In this study, we demonstrate advantages of using custom large-density embryo arrays