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  • Exploring Excitotoxicity and Regulation of a Constitutively Active TRP Ca2+ Channel in Drosophila
    Fly (IF 1.204) Pub Date : 2020-12-01
    Bih-Hwa Shieh; Lucinda Nuzum; Inga Kristaponyte

    ABSTRACT Unregulated Ca2+ influx affects intracellular Ca2+ homoeostasis, which may lead to neuronal death. In Drosophila, following the activation of rhodopsin the TRP Ca2+ channel is open to mediate the light-dependent depolarization. A constitutively active TRP channel triggers the degeneration of TrpP365 /+ photoreceptors. To explore retinal degeneration, we employed a multidisciplinary approach

  • Drosophila melanogaster: a fruitful model for oncohistones
    Fly (IF 1.204) Pub Date : 2021-01-11
    Amel Chaouch; Paul Lasko

    ABSTRACT Drosophila melanogaster has proven to be a powerful genetic model to study human disease. Approximately 75% of human disease-associated genes have homologs in the fruit fly and regulatory pathways are highly conserved in Drosophila compared to humans. Drosophila is an established model organism for the study of genetics and developmental biology related to human disease and has also made a

  • PseudoBase: a genomic visualization and exploration resource for the Drosophila pseudoobscura subgroup
    Fly (IF 1.204) Pub Date : 2021-01-11
    Katharine L. Korunes; Russell B. Myers; Ryan Hardy; Mohamed A. F. Noor

    ABSTRACT Drosophila pseudoobscura is a classic model system for the study of evolutionary genetics and genomics. Given this long-standing interest, many genome sequences have accumulated for D. pseudoobscura and closely related species D. persimilis, D. miranda, and D. lowei. To facilitate the exploration of genetic variation within species and comparative genomics across species, we present PseudoBase

  • Sperm viability varies with buffer and genotype in Drosophila melanogaster
    Fly (IF 1.204) Pub Date : 2020-12-08
    Ruijian Guo; Anna-Lena Henke; Klaus Reinhardt

    ABSTRACT Sperm quality, an important male fitness trait, is commonly compared between studies. However, few studies consider how genetic and environmental variation affect sperm quality, even in the genetic model Drosophila melanogaster. Here we show that sperm viability, the proportion of live sperm, differed across the genotypes Oregon-R, Dahomey, and Canton-S by more than 15%, and across buffers

  • Short is still sweet
    Fly (IF 1.204) Pub Date : 2020-10-19
    Howy Jacobs

    (2020). Short is still sweet. Fly: Vol. 14, No. 1-4, pp. 1-2.

  • A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila
    Fly (IF 1.204) Pub Date : 2020-10-21
    Kevin G. Nyberg; Joseph Q. Nguyen; Yong-Jae Kwon; Shelby Blythe; Greg J. Beitel; Richard Carthew

    ABSTRACT Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out. However, technical challenges remain to improve efficiency and broaden applicability to any genetic background of Drosophila melanogaster as well as to other Drosophila species. To address these issues

  • The effects of genetic background on exercise performance in Drosophila
    Fly (IF 1.204) Pub Date : 2020-10-26
    Deena Damschroder; Kristin Richardson; Tyler Cobb; Robert Wessells

    ABSTRACT The use of the Drosophila model for studying the broad beneficial effects of exercise training has grown over the past decade. As work using Drosophila as an exercise model becomes more widespread, the influence of genetic background on performance should be examined in order to better understand its influence on assessments used to quantitatively measure and compare exercise phenotypes. In

  • SUMO conjugation regulates immune signalling.
    Fly (IF 1.204) Pub Date : 2020-08-31
    Sushmitha Hegde,Amarendranath Soory,Bhagyashree Kaduskar,Girish S Ratnaparkhi

    ABSTRACT Post-translational modifications (PTMs) are critical drivers and attenuators for proteins that regulate immune signalling cascades in host defence. In this review, we explore functional roles for one such PTM, the small ubiquitin-like modifier (SUMO). Very few of the SUMO conjugation targets identified by proteomic studies have been validated in terms of their roles in host defence. Here,

  • Drosophila GFAT1 and GFAT2 enzymes encode obligate developmental functions.
    Fly (IF 1.204) Pub Date : 2020-07-02
    Po Chen,Sarah Visokay,John M Abrams

    ABSTRACT Glutamine: fructose-6-phosphate amidotransferase (GFAT) enzymes catalyse the first committed step of the hexosamine biosynthesis pathway (HBP) using glutamine and fructose-6-phosphate to form glucosamine-6-phosphate (GlcN6P). Numerous species (e.g. mouse, rat, zebrafish, chicken) including humans and Drosophila encode two broadly expressed copies of this enzyme but whether these perform redundant

  • A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants.
    Fly (IF 1.204) Pub Date : 2020-02-09
    Alex Murray,Daniel Palmer,Daimark Bennett,Venkata Dwarampudi,João Pedro de Magalhães

    ABSTRACT The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability

  • The DNA polymerases of Drosophila melanogaster.
    Fly (IF 1.204) Pub Date : 2020-01-14
    Steven J Marygold,Helen Attrill,Elena Speretta,Kate Warner,Michele Magrane,Maria Berloco,Sue Cotterill,Mitch McVey,Yikang Rong,Masamitsu Yamaguchi

    ABSTRACT DNA synthesis during replication or repair is a fundamental cellular process that is catalyzed by a set of evolutionary conserved polymerases. Despite a large body of research, the DNA polymerases of Drosophila melanogaster have not yet been systematically reviewed, leading to inconsistencies in their nomenclature, shortcomings in their functional (Gene Ontology, GO) annotations and an under-appreciation

  • SILAC-based quantitative proteomic analysis of Drosophila gastrula stage embryos mutant for fibroblast growth factor signalling
    Fly (IF 1.204) Pub Date : 2019-12-24
    Hamze Beati; Alistair Langlands; Sara ten Have; H.-Arno J. Müller

    ABSTRACT Quantitative proteomic analyses in combination with genetics provide powerful tools in developmental cell signalling research. Drosophila melanogaster is one of the most widely used genetic models for studying development and disease. Here we combined quantitative proteomics with genetic selection to determine changes in the proteome upon depletion of Heartless (Htl) Fibroblast-Growth Factor

  • Mitochondrial dysfunction generates a growth-restraining signal linked to pyruvate in Drosophila larvae.
    Fly (IF 1.204) Pub Date : 2019-09-17
    Jack George,Tea Tuomela,Esko Kemppainen,Antti Nurminen,Samuel Braun,Cagri Yalgin,Howard T Jacobs

    The Drosophila bang-sensitive mutant tko25t, manifesting a global deficiency in oxidative phosphorylation due to a mitochondrial protein synthesis defect, exhibits a pronounced delay in larval development. We previously identified a number of metabolic abnormalities in tko25t larvae, including elevated pyruvate and lactate, and found the larval gut to be a crucial tissue for the regulation of larval

  • Expansion of a fly TBI model to four levels of injury severity reveals synergistic effects of repetitive injury for moderate injury conditions.
    Fly (IF 1.204) Pub Date : 2019-09-15
    Lauren J Putnam,Ashley M Willes,Brooke E Kalata,Nathaniel D Disher,Douglas J Brusich

    Several million traumatic brain injury (TBI) events are reported in the United States annually. However, mild TBI events often go unreported, and mild and repetitive mild TBI conditions are challenging to model. Fruit flies (Drosophila melanogaster) have gained traction for the study of TBI. The best-characterized fly TBI model is the high-impact trauma (HIT) method. We replicated the HIT method and

  • A standardized nomenclature and atlas of the male terminalia of Drosophila melanogaster.
    Fly (IF 1.204) Pub Date : 2019-08-19
    Gavin Rice,Jean R David,Yoshitaka Kamimura,John P Masly,Alistair P Mcgregor,Olga Nagy,Stéphane Noselli,Maria Daniela Santos Nunes,Patrick O'Grady,Ernesto Sánchez-Herrero,Mark L Siegal,Masanori J Toda,Mark Rebeiz,Virginie Courtier-Orgogozo,Amir Yassin

    Animal terminalia represent some of the most diverse and rapidly evolving structures in the animal kingdom, and for this reason have been a mainstay in the taxonomic description of species. The terminalia of Drosophila melanogaster, with its wide range of experimental tools, have recently become the focus of increased interest in the fields of development, evolution, and behavior. However, studies

  • Bivalency in Drosophila embryos is associated with strong inducibility of Polycomb target genes.
    Fly (IF 1.204) Pub Date : 2019-05-25
    Arslan Akmammedov,Marco Geigges,Renato Paro

    Polycomb group (PcG) and Trithorax group (TrxG) proteins orchestrate development of a multicellular organism by faithfully maintaining cell fate decisions made early in embryogenesis. An important chromatin mark connected to PcG/TrxG regulation is bivalent domains, the simultaneous presence of H3K27me3 and H3K4me3 on a given locus, originally identified in mammalian embryonic stem cells but considered

  • A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila.
    Fly (IF 1.204) Pub Date : 2019-03-28
    Jun Luo,Pingping Shen,Jiong Chen

    The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics. Recently, the Drosophila transgenic technology has been improved by the highly efficient phiC31 site-specific integration system. Many new and improved fluorescent proteins with desirable advantages have been developed. However

  • Maintenance of Rhodopsin levels in Drosophila photoreceptor and phototransduction requires Protein Kinase D.
    Fly (IF 1.204) Pub Date : 2019-02-05
    Sudipta Ashe,Shweta Yadav

    During Drosophila phototransduction, the G protein coupled receptor (GPCR) Rhodopsin (Rh1) transduces photon absorption into electrical signal via G-protein coupled activation of phospholipase C (PLC). Rh1 levels in the plasma membrane are critical for normal sensitivity to light. In this study, we report that Protein Kinase D (dPKD) regulates Rh1 homeostasis in adult photoreceptors. Although eye development

  • Drosophila strain specific response to cisplatin neurotoxicity.
    Fly (IF 1.204) Pub Date : 2019-01-22
    Christopher M Groen,Jewel L Podratz,Kevin Treb,Anthony J Windebank

    Drosophila melanogaster has recently been developed as a simple, in vivo, genetic model of chemotherapy-induced peripheral neuropathy. Flies treated with the chemotherapy agent cisplatin display both a neurodegenerative phenotype and cell death in rapidly dividing follicles, mimicking the cell specific responses seen in humans. Cisplatin induces climbing deficiencies and loss of fertility in a dose

  • A model-based high throughput method for fecundity estimation in fruit fly studies.
    Fly (IF 1.204) Pub Date : 2018-12-30
    Enoch Ng'oma,Elizabeth G King,Kevin M Middleton

    The ability to quantify fecundity is critically important to a wide range of experimental applications, particularly in widely-used model organisms such as Drosophila melanogaster. However, the standard method of manually counting eggs is time consuming and limits the feasibility of large-scale experiments. We develop a predictive model to automate the counting of eggs from images of eggs removed from

  • Functional interplay between ribosomal protein paralogues in the eRpL22 family in Drosophila melanogaster.
    Fly (IF 1.204) Pub Date : 2018-11-29
    Catherine M Mageeney,Michael G Kearse,Brett W Gershman,Caroline E Pritchard,Jennifer M Colquhoun,Vassie C Ware

    Duplicated ribosomal protein (RP) genes in the Drosophila melanogaster eRpL22 family encode structurally-divergent and differentially-expressed rRNA-binding RPs. eRpL22 is expressed ubiquitously and eRpL22-like expression is tissue-restricted with highest levels in the adult male germline. We explored paralogue functional equivalence using the GAL4-UAS system for paralogue knockdown or overexpression

  • Localization and translation control of slam in Drosophila cellularization.
    Fly (IF 1.204) Pub Date : 2018-09-18
    Shuling Yan,Jörg Großhans

    In this extra view, we comment on our recent work concerning the mRNA localization of the gene slow as molasses (slam). slam is a gene essential for the polarized invagination of the plasma membrane and separation of basal and lateral cortical domains during cellularization as well as for germ cell migration in later embryogenesis. We have demonstrated an intimate relationship between slam RNA and

  • Sex-lethal in neurons controls female body growth in Drosophila.
    Fly (IF 1.204) Pub Date : 2018-08-21
    Annick Sawala,Alex P Gould

    Sexual size dimorphism (SSD), a sex difference in body size, is widespread throughout the animal kingdom, raising the question of how sex influences existing growth regulatory pathways to bring about SSD. In insects, somatic sexual differentiation has long been considered to be controlled strictly cell-autonomously. Here, we discuss our surprising finding that in Drosophila larvae, the sex determination

  • bantam microRNA is a negative regulator of the Drosophila decapentaplegic pathway.
    Fly (IF 1.204) Pub Date : 2018-08-19
    Nanci S Kane,Mehul Vora,Richard W Padgett,Ying Li

    Decapentaplegic (Dpp), the Drosophila homolog of the vertebrate bone morphogenetic protein (BMP2/4), is crucial for patterning and growth in many developmental contexts. The Dpp pathway is regulated at many different levels to exquisitely control its activity. We show that bantam (ban), a microRNA, modulates Dpp signaling activity. Over expression of ban decreases phosphorylated Mothers against decapentaplegic

  • Dietary management and physical exercise can improve climbing defects and mitochondrial activity in Drosophila melanogaster parkin null mutants.
    Fly (IF 1.204) Pub Date : 2018-08-01
    Rijan Bajracharya,J William O Ballard

    Physical exercise can improve gait, balance, tremor, flexibility, grip strength and motor coordination in Parkinson’s disease (PD) patients. Several lines of evidence have also shown the therapeutic potential of dietary management and supplementation in halting the progression of PD. However, there is a lack of research on the combined effects of physical activity and nutrition in the progression of

  • Keeping it tight: The relationship between bacterial dysbiosis, septate junctions, and the intestinal barrier in Drosophila.
    Fly (IF 1.204) Pub Date : 2018-03-06
    Martin Resnik-Docampo,Vivien Sauer,Joseph M Schinaman,Rebecca I Clark,David W Walker,D Leanne Jones

    Maladaptive changes in the intestinal flora, typically referred to as bacterial dysbiosis, have been linked to intestinal aging phenotypes, including an increase in intestinal stem cell (ISC) proliferation, activation of inflammatory pathways, and increased intestinal permeability1,2. However, the causal relationships between these phenotypes are only beginning to be unravelled. We recently characterized

  • Epithelial tumors: Growing from within.
    Fly (IF 1.204) Pub Date : 2018-03-01
    Mariana Muzzopappa,Marco Milán

    The growth of epithelial tumors is often governed by cell interactions with the surrounding stroma. Drosophila has been instrumental in identifying the relevant molecular elements mediating these interactions. Of note is the role of the TNF ligand Eiger, released from recruited blood cells, in activating the JNK tumor-promoting pathway in epithelial tumors. JNK drives the transcriptional induction

  • The COP9 signalosome inhibits Cullin-RING E3 ubiquitin ligases independently of its deneddylase activity.
    Fly (IF 1.204) Pub Date : 2018-02-09
    Annabelle Suisse,Miklós Békés,Tony T Huang,Jessica E Treisman

    The COP9 signalosome inhibits the activity of Cullin-RING E3 ubiquitin ligases by removing Nedd8 modifications from their Cullin subunits. Neddylation renders these complexes catalytically active, but deneddylation is also necessary for them to exchange adaptor subunits and avoid auto-ubiquitination. Although deneddylation is thought to be the primary function of the COP9 signalosome, additional activities

  • Combining morphology and molecular data to improve Drosophila paulistorum (Diptera, Drosophilidae) taxonomic status.
    Fly (IF 1.204) Pub Date : 2018-02-07
    Rebeca Zanini,Mário Josias Müller,Gilberto Cavalheiro Vieira,Victor Hugo Valiati,Maríndia Deprá,Vera Lúcia da Silva Valente

    The willistoni species subgroup has been the subject of several studies since the latter half of the past century and is considered a Neotropical model for evolutionary studies, given the many levels of reproductive isolation and different evolutionary stages occurring within them. Here we present for the first time a phylogenetic reconstruction combining morphological characters and molecular data

  • Compromising asymmetric stem cell division in Drosophila central brain: Revisiting the connections with tumorigenesis.
    Fly (IF 1.204) Pub Date : 2018-01-08
    Ana Carmena

    Asymmetric cell division (ACD) is an essential process during development for generating cell diversity. In addition, a more recent connection between ACD, cancer and stem cell biology has opened novel and highly intriguing venues in the field. This connection between compromised ACD and tumorigenesis was first demonstrated using Drosophila neural stem cells (neuroblasts, NBs) more than a decade ago

  • FijiWingsPolarity: An open source toolkit for semi-automated detection of cell polarity.
    Fly (IF 1.204) Pub Date : 2017-12-22
    Leonard L Dobens,Anna Shipman,Jeffrey D Axelrod

    Epithelial cells are defined by apical-basal and planar cell polarity (PCP) signaling, the latter of which establishes an orthogonal plane of polarity in the epithelial sheet. PCP signaling is required for normal cell migration, differentiation, stem cell generation and tissue repair, and defects in PCP have been associated with developmental abnormalities, neuropathologies and cancers. While the molecular

  • Pleiotropy in Drosophila organogenesis: Mechanistic insights from Combgap and the retinal determination gene network.
    Fly (IF 1.204) Pub Date : 2017-12-12
    Trevor L Davis,Ilaria Rebay

    Master regulatory transcription factors cooperate in networks to shepherd cells through organogenesis. In the Drosophila eye, a collection of master control proteins known as the retinal determination gene network (RDGN) switches the direction and targets of its output to choreograph developmental transitions, but the molecular partners that enable such regulatory flexibility are not known. We recently

  • Extending julius seizure, a bang-sensitive gene, as a model for studying epileptogenesis: Cold shock, and a new insertional mutation.
    Fly (IF 1.204) Pub Date : 2017-12-08
    Derek Dean,Hannah Weinstein,Seema Amin,Breelyn Karno,Emma McAvoy,Ronald Hoy,Andrew Recknagel,Casey Jarvis,David Deitcher

    The bang-sensitive (BS) mutants of Drosophila are an important model for studying epilepsy. We recently identified a novel BS locus, julius seizure (jus), encoding a protein containing two transmembrane domains and an extracellular cysteine-rich loop. We also determined that jussda iso7.8, a previously identified BS mutation, is an allele of jus by recombination, deficiency mapping, complementation

  • Strong responses of Drosophila melanogaster microbiota to developmental temperature.
    Fly (IF 1.204) Pub Date : 2017-12-07
    Neda N Moghadam,Pia Mai Thorshauge,Torsten N Kristensen,Nadieh de Jonge,Simon Bahrndorff,Henrik Kjeldal,Jeppe Lund Nielsen

    Physiological responses to changes in environmental conditions such as temperature may partly arise from the resident microbial community that integrates a wide range of bio-physiological aspects of the host. In the present study, we assessed the effect of developmental temperature on the thermal tolerance and microbial community of Drosophila melanogaster. We also developed a bacterial transplantation

  • Identifying the minimum number of microsatellite loci needed to assess population genetic structure: A case study in fly culturing.
    Fly (IF 1.204) Pub Date : 2017-12-01
    Wolfgang Arthofer,Carina Heussler,Patrick Krapf,Birgit C Schlick-Steiner,Florian M Steiner

    Small, isolated populations are constantly threatened by loss of genetic diversity due to drift. Such situations are found, for instance, in laboratory culturing. In guarding against diversity loss, monitoring of potential changes in population structure is paramount; this monitoring is most often achieved using microsatellite markers, which can be costly in terms of time and money when many loci are

  • Deletion of the nicotinic acetylcholine receptor subunit gene Dα1 confers insecticide resistance, but at what cost?
    Fly (IF 1.204) Pub Date : 2017-11-22
    Jason Somers,Hang Ngoc Bao Luong,Philip Batterham,Trent Perry

    Nicotinic acetylcholine receptors (nAChRs) have vital functions in processes of neurotransmission that underpin key behaviors. These pentameric ligand-gated ion channels have been used as targets for insecticides that constitutively activate them, causing the death of insect pests. In examining a knockout of the Dα1 nAChR subunit gene, our study linked this one subunit with multiple traits. We were

  • Control of protein translation by IP3R-mediated Ca2+ release in Drosophila neuroendocrine cells.
    Fly (IF 1.204) Pub Date : 2017-10-11
    Megha,Gaiti Hasan

    The inositol 1,4,5-trisphosphate receptor (IP3R) is one of two Ca2+ channels that gates Ca2+ release from ER-stores. The ligand IP3, generated upon specific G-protein coupled receptor activation, binds to IP3R to release Ca2+ into the cytosol. IP3R also mediates ER-store Ca2+ release into the mitochondria, under basal as well as stimulatory conditions; an activity that influences cellular bioenergetics

  • Dual role of BMP signaling in the regulation of Drosophila intestinal stem cell self-renewal.
    Fly (IF 1.204) Pub Date : 2017-10-11
    Aiguo Tian,Jin Jiang

    Many adult organs including Drosophila adult midguts rely on resident stem cells to replenish damaged cells during tissue homeostasis and regeneration. Previous studies have shown that, upon injury, intestinal stem cells (ISCs) in the midguts can increase proliferation and lineage differentiation to meet the demand for tissue repair. Our recent study has demonstrated that, in response to certain injury

  • Generation of genome-modified Drosophila cell lines using SwAP.
    Fly (IF 1.204) Pub Date : 2017-10-11
    Alexandra Franz,Erich Brunner,Konrad Basler

    The ease of generating genetically modified animals and cell lines has been markedly increased by the recent development of the versatile CRISPR/Cas9 tool. However, while the isolation of isogenic cell populations is usually straightforward for mammalian cell lines, the generation of clonal Drosophila cell lines has remained a longstanding challenge, hampered by the difficulty of getting Drosophila

  • Complex roles of myoglianin in regulating adult performance and lifespan.
    Fly (IF 1.204) Pub Date : 2017-09-14
    Hrvoje Augustin,Jennifer Adcott,Christopher J H Elliott,Linda Partridge

    Myoglianin, the Drosophila homolog of the secreted vertebrate proteins Myostatin and GDF-11, is an important regulator of neuronal modeling, and synapse function and morphology. While Myoglianin suppression during development elicits positive effects on the neuromuscular system, genetic manipulations of myoglianin expression levels have a varied effect on the outcome of performance tests in aging flies

  • Comparative analysis of behavioral and transcriptional variation underlying CO2 sensory neuron function and development in Drosophila.
    Fly (IF 1.204) Pub Date : 2017-07-13
    Jia Wern Pan,Joi McLaughlin,Haining Yang,Charles Leo,Paula Rambarat,Sumie Okuwa,Anaïs Monroy-Eklund,Sabrina Clark,Corbin D Jones,Pelin Cayirlioglu Volkan

    Carbon dioxide is an important environmental cue for many insects, regulating many behaviors including some that have direct human impacts. To further improve our understanding of how this system varies among closely related insect species, we examined both the behavioral response to CO2 as well as the transcriptional profile of key developmental regulators of CO2 sensory neurons in the olfactory system

  • Genomic signatures of local adaptation in the Drosophila immune response.
    Fly (IF 1.204) Pub Date : 2017-06-26
    Angela M Early,Andrew G Clark

    As environments and pathogen landscapes shift, host defenses must evolve to remain effective. Due to this selection pressure, among-species comparisons of genetic sequence data often find immune genes to be among the fastest evolving genes across the genome. The full extent and nature of these immune adaptations, however, remain largely unexplored. In a recent study, we analyzed patterns of selection

  • The dark side of hippo signaling: A cancer promoter role.
    Fly (IF 1.204) Pub Date : 2017-06-21
    Brandon Dunn,Xianjue Ma

    The Hippo signaling pathway regulates organ size and tissue homeostasis. Given this role it is unsurprising that dysregulation of this pathway has implications for cancer progression. A convincing body of literature shows that the Hippo pathway serves a tumor suppressive function with its inactivation leading to massive overgrowth. However, additional studies have also shown that activation of Hippo

  • MTV sings jubilation for telomere biology in Drosophila.
    Fly (IF 1.204) Pub Date : 2017-06-02
    Lin Cheng,Ming Cui,Yikang S Rong

    Telomere protects the ends of linear chromosomes. Telomere dysfunction fuels genome instability that can lead to diseases such as cancer. For over 30 years, Drosophila has fascinated the field as the only major model organism that does not rely on the conserved telomerase enzyme for end protection. Instead of short DNA repeats at chromosome ends, Drosophila has domesticated retrotransposons. In addition

  • New perspectives on the roles of Abl tyrosine kinase in axon patterning.
    Fly (IF 1.204) Pub Date : 2017-05-25
    Ramakrishnan Kannan,Edward Giniger

    The Abelson tyrosine kinase (Abl) lies at the heart of one of the small set of ubiquitous, conserved signal transduction pathways that do much of the work of development and physiology. Abl signaling is essential to epithelial integrity, motility of autonomous cells such as blood cells, and axon growth and guidance in the nervous system. However, though Abl was one of the first of these conserved signaling

  • The absence of crossovers on chromosome 4 in Drosophila melanogaster: Imperfection or interesting exception?
    Fly (IF 1.204) Pub Date : 2017-05-04
    Michaelyn A Hartmann,Jeff Sekelsky

    Drosophila melanogaster chromosome 4 is an anomaly because of its small size, chromatin structure, and most notably its lack of crossing over during meiosis. Earlier ideas about the absence of crossovers on 4 hypothesize that these unique characteristics function to prevent crossovers. Here, we explore hypotheses about the absence of crossovers on 4, how these have been addressed, and new insights

  • Cells on the move: Modulation of guidance cues during germ cell migration.
    Fly (IF 1.204) Pub Date : 2017-03-31
    Girish Deshpande,Justinn Barr,Offer Gerlitz,Lyubov Lebedeva,Yulii Shidlovskii,Paul Schedl

    In Drosophila melanogaster the progenitors of the germ-line stem cells, the primordial germ cells (PGCs) are formed on the outside surface of the early embryo, while the somatic gonadal precursor cells (SGPs) are specified during mid-embryogenesis. To form the primitive embryonic gonad, the PGCs travel from outside of the embryo, across the mid-gut and then migrate through the mesoderm to the SGPs

  • Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition.
    Fly (IF 1.204) Pub Date : 2017-03-24
    Kiel G Ormerod,Olivia K LePine,Prabhodh S Abbineni,Justin M Bridgeman,Jens R Coorssen,A Joffre Mercier,Glenn J Tattersall

    Diet profoundly influences the behavior of animals across many phyla. Despite this, most laboratories using model organisms, such as Drosophila, use multiple, different, commercial or custom-made media for rearing their animals. In addition to measuring growth, fecundity and longevity, we used several behavioral and physiological assays to determine if and how altering food media influence wild-type

  • Methods to investigate the structure and connectivity of the nervous system.
    Fly (IF 1.204) Pub Date : 2017-03-24
    Donghyung Lee,Ting-Hao Huang,Aubrie De La Cruz,Antuca Callejas,Carlos Lois

    Understanding the computations that take place in neural circuits requires identifying how neurons in those circuits are connected to one another. In addition, recent research indicates that aberrant neuronal wiring may be the cause of several neurodevelopmental disorders, further emphasizing the importance of identifying the wiring diagrams of brain circuits. To address this issue, several new approaches

  • Monitoring the effect of pathogenic nematodes on locomotion of Drosophila larvae.
    Fly (IF 1.204) Pub Date : 2017-03-03
    Martin Kunc,Badrul Arefin,Pavel Hyrsl,Ulrich Theopold

    One of the key factors that determine the interaction between hosts and their parasites is the frequency of their interactions, which depends on the locomotory behavior of both parts. To address host behavior we used natural infections involving insect pathogenic nematodes and Drosophila melanogaster larvae as hosts. Using a modified version of a recently described method (FIMTrack) to assess several

  • Gene expression and morphogenesis during the deposition of Drosophila wing cuticle.
    Fly (IF 1.204) Pub Date : 2017-03-01
    Paul N Adler

    The exoskeleton of insects and other arthropods is a very versatile material that is characterized by a complex multilayer structure. In Sobala and Adler (2016) we analyzed the process of wing cuticle deposition by RNAseq and electron microscopy. In this extra view we discuss the unique aspects of the envelope the first and most outermost layer and the gene expression program seen at the end of cuticle

  • secHsp70 as a tool to approach amyloid-β42 and other extracellular amyloids.
    Fly (IF 1.204) Pub Date : 2017-02-26
    Lorena De Mena,Deepak Chhangani,Pedro Fernandez-Funez,Diego E Rincon-Limas

    Self-association of amyloidogenic proteins is the main pathological trigger in a wide variety of neurodegenerative disorders. These aggregates are deposited inside or outside the cell due to hereditary mutations, environmental exposures or even normal aging. Cumulative evidence indicates that the heat shock chaperone Hsp70 possesses robust neuroprotection against various intracellular amyloids in Drosophila

  • Parsimony and complexity: Cell fate assignment in the developing Drosophila eye.
    Fly (IF 1.204) Pub Date : 2017-02-23
    Yannis Emmanuel Mavromatakis,Andrew Tomlinson

    The specification of the R7 photoreceptor in the Drosophila eye has become a classic model for understanding how cell fates are assigned in developing systems. R7 is derived from a group of cells that also gives rise to the R1/6 photoreceptor class and the non-photoreceptor cone cells. Our studies examine the signals and cellular information that direct each of these cell types. The cell fates are

  • Ovarian ecdysteroid biosynthesis and female germline stem cells.
    Fly (IF 1.204) Pub Date : 2017-02-21
    Tomotsune Ameku,Yuto Yoshinari,Ruriko Fukuda,Ryusuke Niwa

    The germline stem cells (GSCs) are critical for gametogenesis throughout the adult life. Stem cell identity is maintained by local signals from a specialized microenvironment called the niche. However, it is unclear how systemic signals regulate stem cell activity in response to environmental cues. In our previous article, we reported that mating stimulates GSC proliferation in female Drosophila. The

  • Gluconeogenesis: An ancient biochemical pathway with a new twist.
    Fly (IF 1.204) Pub Date : 2017-02-07
    Tetsuya Miyamoto,Hubert Amrein

    Synthesis of sugars from simple carbon sources is critical for survival of animals under limited nutrient availability. Thus, sugar-synthesizing enzymes should be present across the entire metazoan spectrum. Here, we explore the evolution of glucose and trehalose synthesis using a phylogenetic analysis of enzymes specific for the two pathways. Our analysis reveals that the production of trehalose is

  • From the editors.
    Fly (IF 1.204) Pub Date : 2017-02-06

    (2017). From the editors. Fly: Vol. 11, No. 1, pp. 1-2.

  • Mobile-phone radiation-induced perturbation of gene-expression profiling, redox equilibrium and sporadic-apoptosis control in the ovary of Drosophila melanogaster.
    Fly (IF 1.204) Pub Date : 2017-01-12
    Areti K Manta,Deppie Papadopoulou,Alexander P Polyzos,Adamantia F Fragopoulou,Aikaterini S Skouroliakou,Dimitris Thanos,Dimitrios J Stravopodis,Lukas H Margaritis

    The daily use by people of wireless communication devices has increased exponentially in the last decade, begetting concerns regarding its potential health hazards. Drosophila melanogaster four days-old adult female flies were exposed for 30 min to radiation emitted by a commercial mobile phone at a SAR of 0.15 W/kg and a SAE of 270 J/kg. ROS levels and apoptotic follicles were assayed in parallel

  • We also CanFly! The 2nd MexFly drosophila research conference.
    Fly (IF 1.204) Pub Date : 2017-01-09
    Fanis Missirlis,Marcos Nahmad

    The 2nd Mexican Drosophila Research Conference (MexFly) took place on June 30th and July 1st, 2016 in Mexico City, at the Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav). Principal investigators, postdocs, students, and technicians from Drosophila labs across Mexico attended. The guest speaker was Chris Rushlow from New York University, who presented work

  • What does the fruitless gene tell us about nature vs. nurture in the sex life of Drosophila?
    Fly (IF 1.204) Pub Date : 2016-12-18
    Daisuke Yamamoto,Soh Kohatsu

    The fruitless (fru) gene in Drosophila has been proposed to play a master regulator role in the formation of neural circuitries for male courtship behavior, which is typically considered to be an innate behavior composed of a fixed action pattern as generated by the central pattern generator. However, recent studies have shed light on experience-dependent changes and sensory-input-guided plasticity

  • Strategy changes in subsequent fights as consequences of winning and losing in fruit fly fights.
    Fly (IF 1.204) Pub Date : 2016-12-02
    Séverine Trannoy,Edward A Kravitz

    In competition for food, territory and mates, male fruit flies (Drosophila melanogaster) engage in agonistic encounters with conspecifics. The fighting strategies used to obtain these resources are influenced by previous and present experience, environmental cues, and the internal state of the animal including hormonal and genetic influences. Animals that experience prior defeats show submissive behavior

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