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Interleukin-6 promotes primitive endoderm development in bovine blastocysts BMC Dev. Biol. (IF 2.0) Pub Date : 2021-01-12 Lydia K. Wooldridge; Alan D. Ealy
Interleukin-6 (IL6) was recently identified as an embryotrophic factor in bovine embryos, where it acts primarily to mediate inner cell mass (ICM) size. This work explored whether IL6 affects epiblast (EPI) and primitive endoderm (PE) development, the two embryonic lineages generated from the ICM after its formation. Nuclear markers for EPI (NANOG) and PE (GATA6) were used to differentiate the two
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Dynamic conversion of cell sorting patterns in aggregates of embryonic stem cells with differential adhesive affinity BMC Dev. Biol. (IF 2.0) Pub Date : 2021-01-06 Jeffrey D. Tse; Robert Moore; Yue Meng; Wensi Tao; Elizabeth R. Smith; Xiang-Xi Xu
Mammalian early development comprises the proliferation, differentiation, and self-assembly of the embryonic cells. The classic experiment undertaken by Townes and Holtfreter demonstrated the ability of dissociated embryonic cells to sort and self-organize spontaneously into the original tissue patterns. Here, we further explored the principles and mechanisms underlying the phenomenon of spontaneous
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Effect of inorganic phosphate on migration and osteogenic differentiation of bone marrow mesenchymal stem cells BMC Dev. Biol. (IF 2.0) Pub Date : 2021-01-06 Hengzhang Lin; Yong Zhou; Qun Lei; Dong Lin; Jiang Chen; Chuhuo Wu
Phosphate is the major ingredient of bone tissue, and is also an important component of commercial bone substitute materials, bone scaffolds, and implant surface coatings. With the dissolution of the bone substitute materials and the degradation by cells, local ion concentrations will change and affect bone tissue reconstruction. Bone marrow -derived mesenchymal stem cells (BM-MSCs) are main autologous
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Expression patterns of ciliopathy genes ARL3 and CEP120 reveal roles in multisystem development BMC Dev. Biol. (IF 2.0) Pub Date : 2020-12-09 L. Powell; M. Barroso-Gil; G. J. Clowry; L. A. Devlin; E. Molinari; S. A. Ramsbottom; C. G. Miles; J. A. Sayer
Joubert syndrome and related disorders (JSRD) and Jeune syndrome are multisystem ciliopathy disorders with overlapping phenotypes. There are a growing number of genetic causes for these rare syndromes, including the recently described genes ARL3 and CEP120. We sought to explore the developmental expression patterns of ARL3 and CEP120 in humans to gain additional understanding of these genetic conditions
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Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.) BMC Dev. Biol. (IF 2.0) Pub Date : 2020-12-02 Li Wen; Wei Li; Stephen Parris; Matthew West; John Lawson; Michael Smathers; Zhigang Li; Don Jones; Shuangxia Jin; Christopher A. Saski
Genotype independent transformation and whole plant regeneration through somatic embryogenesis relies heavily on the intrinsic ability of a genotype to regenerate. The critical genetic architecture of non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells in a highly regenerable cotton genotype is unknown. In this study, gene expression profiles of a highly regenerable Gossypium hirsutum
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Label-free quantitative proteomic analysis of insect larval and metamorphic molts BMC Dev. Biol. (IF 2.0) Pub Date : 2020-11-24 Weiye Si; Qingjie Wang; Yu Li; Dujuan Dong
Molting is an essential biological process occurring characteristic times throughout the life cycle of holometabolous insects. However, it is not clear how insects determine the direction of molting to remain status quo or to initiate metamorphosis. To explore the functional factors that determine the direction of molts, liquid chromatography-mass spectrometry was used to identify the molecules involved
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Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas BMC Dev. Biol. (IF 2.0) Pub Date : 2020-11-23 Susanne Vogeler; Stefano Carboni; Xiaoxu Li; Nancy Nevejan; Sean J. Monaghan; Jacqueline H. Ireland; Alyssa Joyce
Nitric oxide (NO) is presumed to be a regulator of metamorphosis in many invertebrate species, and although NO pathways have been comparatively well-investigated in gastropods, annelids and crustaceans, there has been very limited research on the effects of NO on metamorphosis in bivalve shellfish. In this paper, we investigate the effects of NO pathway inhibitors and NO donors on metamorphosis induction
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Depletion of SNRNP200 inhibits the osteo−/dentinogenic differentiation and cell proliferation potential of stem cells from the apical papilla BMC Dev. Biol. (IF 2.0) Pub Date : 2020-11-18 Xiaomin Su; Haoqing Yang; Ruitang Shi; Chen Zhang; Huina Liu; Zhipeng Fan; Jianpeng Zhang
Tissue regeneration mediated by mesenchymal stem cells (MSCs) is deemed a desirable way to repair teeth and craniomaxillofacial tissue defects. Nevertheless, the molecular mechanisms about cell proliferation and committed differentiation of MSCs remain obscure. Previous researches have proved that lysine demethylase 2A (KDM2A) performed significant function in the regulation of MSC proliferation and
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Differences among families in craniofacial shape at early life-stages of Arctic charr (Salvelinus alpinus) BMC Dev. Biol. (IF 2.0) Pub Date : 2020-10-26 Samantha V. Beck; Katja Räsänen; Camille A. Leblanc; Skúli Skúlason; Zophonías O. Jónsson; Bjarni K. Kristjánsson
Organismal fitness can be determined at early life-stages, but phenotypic variation at early life-stages is rarely considered in studies on evolutionary diversification. The trophic apparatus has been shown to contribute to sympatric resource-mediated divergence in several taxa. However, processes underlying diversification in trophic traits are poorly understood. Using phenotypically variable Icelandic
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Monocyte Chemoattractant Protein-1 stimulates the differentiation of rat stem and progenitor Leydig cells during regeneration BMC Dev. Biol. (IF 2.0) Pub Date : 2020-10-06 Xiangcheng Zhan; Jingwei Zhang; Saiyang Li; Xiaolu Zhang; Linchao Li; Tiantian Song; Qunlong Liu; Jun Lu; Yunfei Xu; Ren-Shan Ge
Monocyte chemoattractant protein-1(MCP-1) is a chemokine secreted by Leydig cells and peritubular myoid cells in the rat testis. Its role in regulating the development of Leydig cells via autocrine and paracrine is still unclear. The objective of the current study was to investigate the effects of MCP-1 on Leydig cell regeneration from stem cells in vivo and on Leydig cell development in vitro. Intratesticular
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Transgenic and knockout analyses of Masculinizer and doublesex illuminated the unique functions of doublesex in germ cell sexual development of the silkworm, Bombyx mori. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-09-21 Tomohisa Yuzawa,Misato Matsuoka,Megumi Sumitani,Fugaku Aoki,Hideki Sezutsu,Masataka G Suzuki
Masculinizer (Masc) plays a pivotal role in male sex determination in the silkworm, Bombyx mori. Masc is required for male-specific splicing of B. mori doublesex (Bmdsx) transcripts. The male isoform of Bmdsx (BmdsxM) induces male differentiation in somatic cells, while females express the female isoform of Bmdsx (BmdsxF), which promotes female differentiation in somatic cells. Our previous findings
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Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-08-19 Weihong Yang,Pin Huan,Baozhong Liu
The morphogenesis of the shell field is an essential step of molluscan shell formation, which exhibits both conserved features and interlineage variations. As one major gastropod lineage, the patellogastropods show different characters in its shell field morphogenesis compared to other gastropods (e.g., the pulmonate gastropod Lymnaea stagnalis), likely related to its epibolic gastrulation. The investigation
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Neural defects caused by total and Wnt1-Cre mediated ablation of p120ctn in mice. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-08-03 Tim Pieters,Ellen Sanders,Huiyu Tian,Jolanda van Hengel,Frans van Roy
p120 catenin (p120ctn) is an important component in the cadherin-catenin cell adhesion complex because it stabilizes cadherin-mediated intercellular junctions. Outside these junctions, p120ctn is actively involved in the regulation of small GTPases of the Rho family, in actomyosin dynamics and in transcription regulation. We and others reported that loss of p120ctn in mouse embryos results in an embryonic
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Evolutionary and developmental considerations of the diet and gut morphology in ceratophryid tadpoles (Anura). BMC Dev. Biol. (IF 2.0) Pub Date : 2020-07-29 Marissa Fabrezi,Julio César Cruz
Before metamorphosis, almost all anuran tadpoles are omnivores. Larval carnivory occurs in some species and, it is associated with distinctive morphotypes. Obligatory carnivorous tadpoles exhibit structural changes in the gastrointestinal tract compared to larvae that are predominately omnivores. The most distinctive feature of the anuran family Ceratophyridae (three genera) overall is the enormous
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Analysing bioelectrical phenomena in the Drosophila ovary with genetic tools: tissue-specific expression of sensors for membrane potential and intracellular pH, and RNAi-knockdown of mechanisms involved in ion exchange. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-07-08 Susanne Katharina Schotthöfer,Johannes Bohrmann
Changes in transcellular bioelectrical patterns are known to play important roles during developmental and regenerative processes. The Drosophila follicular epithelium has proven to be an appropriate model system for studying the mechanisms by which bioelectrical signals emerge and act. Fluorescent indicator dyes in combination with various inhibitors of ion-transport mechanisms have been used to investigate
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Identification of vital candidate microRNA/mRNA pairs regulating ovule development using high-throughput sequencing in hazel. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-07-01 Jianfeng Liu,Qizheng Luo,Xingzheng Zhang,Qiang Zhang,Yunqing Cheng
Hazels (Corylus spp.) are economically important nut-producing species in which ovule development determines seed plumpness, one of the key parameters reflecting nut quality. microRNAs (miRNAs) play important roles in RNA silencing and the post-transcriptional regulation of gene expression. However, very little is currently known regarding the miRNAs involved in regulating ovule growth and development
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Wingless and Archipelago, a fly E3 ubiquitin ligase and a homolog of human tumor suppressor FBW7, show an antagonistic relationship in wing development. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-06-29 Sujin Nam,Kyung-Ok Cho
Archipelago (Ago) is a Drosophila homolog of mammalian F-box and WD repeat domain-containing 7 (FBW7, also known as FBXW7). In previous studies, FBW7 has been addressed as a tumor suppressor mediating ubiquitin-dependent proteolysis of several oncogenic proteins. Ubiquitination is a type of protein modification that directs protein for degradation as well as sorting. The level of beta-catenin (β-cat)
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Multiple functions of the scaffold protein Discs large 5 in the control of growth, cell polarity and cell adhesion in Drosophila melanogaster. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-06-18 Parvathy Venugopal,Hugo Veyssière,Jean-Louis Couderc,Graziella Richard,Caroline Vachias,Vincent Mirouse
Scaffold proteins support a variety of key processes during animal development. Mutant mouse for the MAGUK protein Discs large 5 (Dlg5) presents a general growth impairment and moderate morphogenetic defects. Here, we generated null mutants for Drosophila Dlg5 and show that it owns similar functions in growth and epithelial architecture. Dlg5 is required for growth at a cell autonomous level in several
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Notch signaling in mouse blastocyst development and hatching. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-06-02 Mariana R Batista,Patrícia Diniz,Ana Torres,Daniel Murta,Luís Lopes-da-Costa,Elisabete Silva
Mammalian early embryo development requires a well-orchestrated interplay of cell signaling pathways. Notch is a major regulatory pathway involved in cell-fate determination in embryonic and adult scenarios. However, the role of Notch in embryonic pre-implantation development is controversial. In particular, Notch role on blastocyst development and hatching remains elusive, and a complete picture of
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Ephrin-B2 paces neuronal production in the developing neocortex. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-05-13 Anthony Kischel,Christophe Audouard,Mohamad-Ali Fawal,Alice Davy
BACKGROUND During mammalian cerebral cortex development, different types of projection neurons are produced in a precise temporal order and in stereotypical numbers. The mechanisms regulating timely generation of neocortex projection neurons and ensuring production in sufficient numbers of each neuronal identity are only partially understood. RESULTS Here, we show that ephrin-B2, a member of the Eph:ephrin
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High expressions of the cytoglobin and PGC-1α genes during the tissue regeneration of house gecko (Hemidactylus platyurus) tails. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-05-11 Titta Novianti,Vetnizah Juniantito,Ahmad Aulia Jusuf,Evy Ayu Arida,Mohamad Sadikin,Sri Widia A Jusman
BACKGROUND The tissue regeneration process requires high oxygen and energy levels. Cytoglobin (Cygb) is a member of the globin family, which has the ability to bind oxygen, plays a role in dealing with oxidative stress, and carries oxygen into the mitochondria. Energy production for tissue regeneration is associated with mitochondria-especially mitochondrial biogenesis. The peroxisome proliferator-activated
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The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-04-22 Gongyan Liu,Shu Li,Hongli Liu,Yanli Zhu,Liya Bai,Haitao Sun,Shuxia Gao,Wenxue Jiang,Fuchang Li
BACKGROUND Hair follicles are an appendage of the vertebrate epithelium in the skin that arise from the embryonic ectoderm and regenerate cyclically during adulthood. Dermal papilla cells (DPCs) are the key dermal component of the hair follicle that directly regulate hair follicle development, growth and regeneration. According to recent studies, miRNAs play an important role in regulating hair follicle
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A practical staging atlas to study embryonic development of Octopus vulgaris under controlled laboratory conditions. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-04-16 Astrid Deryckere,Ruth Styfhals,Erica A G Vidal,Eduardo Almansa,Eve Seuntjens
BACKGROUND Octopus vulgaris has been an iconic cephalopod species for neurobiology research as well as for cephalopod aquaculture. It is one of the most intelligent and well-studied invertebrates, possessing both long- and short-term memory and the striking ability to perform complex cognitive tasks. Nevertheless, how the common octopus developed these uncommon features remains enigmatic. O. vulgaris
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Butterfly eyespot color pattern formation requires physical contact of the pupal wing epithelium with extracellular materials for morphogenic signal propagation. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-03-31 Joji M Otaki
Eyespot color pattern formation on butterfly wings is sensitive to physical damage and physical distortion as well as physical contact with materials on the surface of wing epithelial tissue at the pupal stage. Contact-mediated eyespot color pattern changes may imply a developmental role of the extracellular matrix in morphogenic signal propagation. Here, we examined eyespot responses to various contact
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Bioelectrical and cytoskeletal patterns correlate with altered axial polarity in the follicular epithelium of the Drosophila mutant gurken BMC Dev. Biol. (IF 2.0) Pub Date : 2020-03-13 Susanne Katharina Schotthöfer; Johannes Bohrmann
Bioelectrical signals are known to be involved in the generation of cell and tissue polarity as well as in cytoskeletal dynamics. The epithelium of Drosophila ovarian follicles is a suitable model system for studying connections between electrochemical gradients, patterns of cytoskeletal elements and axial polarity. By interactions between soma and germline cells, the transforming growth factor-α homolog
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Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-02-26 Nikitas Georgakopoulos,Nicole Prior,Brigitte Angres,Gianmarco Mastrogiovanni,Alex Cagan,Daisy Harrison,Christopher J Hindley,Robert Arnes-Benito,Siong-Seng Liau,Abbie Curd,Natasha Ivory,Benjamin D Simons,Inigo Martincorena,Helmut Wurst,Kourosh Saeb-Parsy,Meritxell Huch
BACKGROUND Pancreatic organoid systems have recently been described for the in vitro culture of pancreatic ductal cells from mouse and human. Mouse pancreatic organoids exhibit unlimited expansion potential, while previously reported human pancreas organoid (hPO) cultures do not expand efficiently long-term in a chemically defined, serum-free medium. We sought to generate a 3D culture system for long-term
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Keratin 86 is up-regulated in the uterus during implantation, induced by oestradiol. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-02-07 He Zhang,Huashan Zhao,Xi Wang,Xiaolin Cui,Lingling Jin
BACKGROUND Uterine receptivity is one of the determinants of embryo implantation, which is responsible for pregnancy success. Aberrant embryo implantation due to disrupted uterine receptivity is usually found in ovarian hyperstimulation induced hyperoestrogen patients. RESULTS This study identified keratin 86 (KRT86), a fibrous structural protein, which was upregulated in uterine endometrium during
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Downregulation of lncRNA DANCR promotes osteogenic differentiation of periodontal ligament stem cells. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-01-14 Zhuo Wang,Yuanliang Huang,Luanjun Tan
BACKGROUND Long non-coding RNAs (lncRNAs) have been widely known to have an appreciable effect in physiology and pathology. In tooth regeneration, periodontal ligament stem cells (PDLSCs) are regarded as a key effector, whereas, how lncRNA acts in the osteogenic differentiation of PDLSCs have not been completely understood. This study aims to find out the relationship between lncRNA DANCR and the proliferation
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Spontaneous embryo resorption in the mouse is triggered by embryonic apoptosis followed by rapid removal via maternal sterile purulent inflammation. BMC Dev. Biol. (IF 2.0) Pub Date : 2020-01-09 Barbara Drews,Luis Flores Landaverde,Anja Kühl,Ulrich Drews
BACKGROUND In normal mammalian development a high percentage of implantations is lost by spontaneous resorption. This is a major problem in assisted reproduction and blastocyst transfer. Which embryo will be resorbed is unpredictable. Resorption is very fast, so that with conventional methods only final haemorrhagic stages are encountered. Here we describe the histology and immunohistochemistry of
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Expression analysis of Rab11 during zebrafish embryonic development. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-12-30 Haijun Zhang,Yu Gao,Peipei Qian,Zhangji Dong,Wenjin Hao,Dong Liu,Xuchu Duan
Rab proteins are GTPases responsible for intracellular vesicular trafficking regulation. Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. In zebrafish, there are 3 rab11 paralogues, known as rab11a, rab11ba and rab11bb, sharing high identity with each other. However, the expression analysis of rab11 is so far lacking. Here, by
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Comparison of the transcriptomes of two tardigrades with different hatching coordination. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-12-09 Yuki Yoshida,Kenta Sugiura,Masaru Tomita,Midori Matsumoto,Kazuharu Arakawa
Tardigrades are microscopic organisms, famous for their tolerance against extreme environments. The establishment of rearing systems of multiple species has allowed for comparison of tardigrade physiology, in particular in embryogenesis. Interestingly, in-lab cultures of limnic species showed smaller variation in hatching timing than terrestrial species, suggesting a hatching regulation mechanism acquired
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DNA methylation and miRNA-1296 act in concert to mediate spatiotemporal expression of KPNA7 during bovine oocyte and early embryonic development. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-12-02 Lei Wang,Jacqelyn M Hand,Liyuan Fu,George W Smith,Jianbo Yao
BACKGROUND Epigenetic regulation of oocyte-specific maternal factors is essential for oocyte and early embryonic development. KPNA7 is an oocyte-specific maternal factor, which controls transportation of nuclear proteins important for early embryonic development. To elucidate the epigenetic mechanisms involved in the controlled expression of KPNA7, both DNA methylation associated transcriptional silencing
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Electrochemical gradients are involved in regulating cytoskeletal patterns during epithelial morphogenesis in the Drosophila ovary. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-11-12 Isabel Weiß,Johannes Bohrmann
BACKGROUND During Drosophila oogenesis, the follicular epithelium differentiates into several morphologically distinct follicle-cell populations. Characteristic bioelectrical properties make this tissue a suitable model system for studying connections between electrochemical signals and the organisation of the cytoskeleton. Recently, we have described stage-specific transcellular antero-posterior and
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Survival of Polyploid hybrid salamander embryos. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-11-12 Noah D Charney,Jacob E Kubel,Craig T Woodard,Blanca I Carbajal-González,Samantha Avis,Julia A Blyth,Charles S Eiseman,John Castorino,John H Malone
BACKGROUND Animals with polyploid, hybrid nuclei offer a challenge for models of gene expression and regulation during embryogenesis. To understand how such organisms proceed through development, we examined the timing and prevalence of mortality among embryos of unisexual salamanders in the genus Ambystoma. RESULTS Our regional field surveys suggested that heightened rates of embryo mortality among
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YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-10-22 Alaa A Eisa,Santanu De,Ariana Detwiler,Eva Gilker,Alexander C Ignatious,Srinivasan Vijayaraghavan,Douglas Kline
BACKGROUND Immature mammalian oocytes are held arrested at prophase I of meiosis by an inhibitory phosphorylation of cyclin-dependent kinase 1 (CDK1). Release from this meiotic arrest and germinal vesicle breakdown is dependent on dephosphorylation of CDK1 by the protein, cell cycle division 25B (CDC25B). Evidence suggests that phosphorylated CDC25B is bound to YWHA (14-3-3) proteins in the cytoplasm
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A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-10-07 Shweta Varshney,Hua-Xing Wei,Frank Batista,Mohd Nauman,Subha Sundaram,Katherine Siminovitch,Ankit Tanwar,Pamela Stanley
BACKGROUND Mouse NOTCH1 carries a highly conserved O-fucose glycan at Thr466 in epidermal growth factor-like repeat 12 (EGF12) of the extracellular domain. O-Fucose at this site has been shown by X-ray crystallography to be recognized by both DLL4 and JAG1 Notch ligands. We previously showed that a Notch1 Thr466Ala mutant exhibits very little ligand-induced NOTCH1 signaling in a reporter assay, whereas
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Sonic hedgehog promotes chondrogenesis of rabbit bone marrow stem cells in a rotary cell culture system. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-08-12 Liyang Chen,Gejun Liu,Wenjun Li,Xing Wu
BACKGROUND Sonic hedgehog (Shh) is an important signalling protein involved in the induction of early cartilaginous differentiation. Herein, we demonstrate that Shh markedly induces chondrogenesis of rabbit bone marrow stromal cells (BMSCs) under microgravity conditions, and promotes cartilage regeneration. RESULTS In the rotary cell culture system (RCCS), chondrogenic differentiation was revealed
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Role of PRY-1/Axin in heterochronic miRNA-mediated seam cell development. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-07-15 Avijit Mallick,Ayush Ranawade,Bhagwati P Gupta
BACKGROUND Caenorhabditis elegans seam cells serve as a good model to understand how genes and signaling pathways interact to control asymmetric cell fates. The stage-specific pattern of seam cell division is coordinated by a genetic network that includes WNT asymmetry pathway components WRM-1, LIT-1, and POP-1, as well as heterochronic microRNAs (miRNAs) and their downstream targets. Mutations in
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Deletion of Tet proteins results in quantitative disparities during ESC differentiation partially attributable to alterations in gene expression. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-07-08 Michael Reimer,Kirthi Pulakanti,Linzheng Shi,Alex Abel,Mingyu Liang,Subramaniam Malarkannan,Sridhar Rao
BACKGROUND The Tet protein family (Tet1, Tet2, and Tet3) regulate DNA methylation through conversion of 5-methylcytosine to 5-hydroxymethylcytosine which can ultimately result in DNA demethylation and play a critical role during early mammalian development and pluripotency. While multiple groups have generated knockouts combining loss of different Tet proteins in murine embryonic stem cells (ESCs)
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Importance of prostate androgen-regulated mucin-like protein 1 in development of the bovine blastocyst. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-07-05 Adriana M Zolini,Verónica M Negrón-Pérez,Peter J Hansen
BACKGROUND Prostate androgen-regulated mucin-like protein 1 (PARM1) is a pro-proliferative and anti-apoptotic glycoprotein involved in the endoplasmic reticulum (ER) stress response. A single nucleotide polymorphism in the coding region of PARM1 has been associated with competence of bovine embryos to develop to the blastocyst stage. Here we tested the importance of PARM1 for development by evaluating
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Insulin signaling mediates previtellogenic development and enhances juvenile hormone-mediated vitellogenesis in a lepidopteran insect, Maruca vitrata. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-07-05 Md Abdullah Al Baki,Dae-Weon Lee,Jin Kyo Jung,Yonggyun Kim
BACKGROUND Insulin/insulin-like growth peptide signaling (IIS) down-regulates hemolymph sugar level and facilitates larval growth in the soybean pod borer, Maruca vitrata. The objective of this study is to determine whether IIS of M. vitrata can mediate ovarian development of adult females. RESULTS A pair of ovaries consists of 8 ovarioles, each of which is separated into distal germarium and proximal
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A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-07-04 Jesica R Canizo,Amada E Ynsaurralde Rivolta,Camila Vazquez Echegaray,Mariana Suvá,Virgilia Alberio,Juan F Aller,Alejandra S Guberman,Daniel F Salamone,Ricardo H Alberio,Ramiro Alberio
BACKGROUND The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been partially studied, and evidence shows that MEK signalling plays a limited role in the segregation of this lineage. Here we explored the role of different signalling pathways during lineage
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Electrochemical patterns during Drosophila oogenesis: ion-transport mechanisms generate stage-specific gradients of pH and membrane potential in the follicle-cell epithelium. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-06-21 Isabel Weiß,Johannes Bohrmann
BACKGROUND Alterations of bioelectrical properties of cells and tissues are known to function as wide-ranging signals during development, regeneration and wound-healing in several species. The Drosophila follicle-cell epithelium provides an appropriate model system for studying the potential role of electrochemical signals, like intracellular pH (pHi) and membrane potential (Vmem), during development
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Growth and cellular patterning during fetal human inner ear development studied by a correlative imaging approach. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-05-20 Lejo Johnson Chacko,David Wertjanz,Consolato Sergi,Jozsef Dudas,Natalie Fischer,Theresa Eberharter,Romed Hoermann,Rudolf Glueckert,Helga Fritsch,Helge Rask-Andersen,Anneliese Schrott-Fischer,Stephan Handschuh
BACKGROUND Progressive transformation of the otic placode into the functional inner ear during gestational development in humans leads to the acquisition of hearing perception via the cochlea and balance and spatial orientation via the vestibular organ. RESULTS Using a correlative approach involving micro-computerized tomography (micro-CT), transmission electron microscopy and histological techniques
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An annual cycle of gene regulation in the red-legged salamander mental gland: from hypertrophy to expression of rapidly evolving pheromones. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-04-27 Damien B Wilburn,Richard C Feldhoff
BACKGROUND Cell differentiation is mediated by synchronized waves of coordinated expression for hundreds to thousands of genes, and must be regulated to produce complex tissues and phenotypes. For many animal species, sexual selection has driven the development of elaborate male ornaments, requiring sex-specific differentiation pathways. One such male ornament is the pheromone-producing mental gland
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Molecular cloning and characteristics of DnaJa1and DnaJb1 in Coilia nasus: possible function involved in oogenesis during spawning migration. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-04-25 Xiao-Ting Feng,Xue-Jun Yang,Jian-Jian Ruan,Ya-Qi Wang,Yan-Feng Zhou,Dong-Po Xu,Di-An Fang
BACKGROUND Coilia nasus oogenesis/spawning migration is a well-defined synchronous arrangement process. DnaJs are indispensable molecular chaperones for oogenesis process. However, how DnaJs involved the anadromous spawning migration mechanism is outstanding and plausible. RESULTS In this regard, two DnaJs (Cn-DnaJa1 and Cn-DnaJb1) are cloned from the Coilia nasus's ovary. Their structure both contains
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Androgen receptor expression is required to ensure development of adult Leydig cells and to prevent development of steroidogenic cells with adrenal characteristics in the mouse testis. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-04-17 Peter J O'Shaughnessy,Rod T Mitchell,Ana Monteiro,Laura O'Hara,Lyndsey Cruickshanks,Hedi Claahsen-van der Grinten,Pamela Brown,Margaret Abel,Lee B Smith
BACKGROUND The interstitium of the mouse testis contains Leydig cells and a small number of steroidogenic cells with adrenal characteristics which may be derived from the fetal adrenal during development or may be a normal subset of the developing fetal Leydig cells. Currently it is not known what regulates development and/or proliferation of this sub-population of steroidogenic cells in the mouse
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Expression of NK genes that are not part of the NK cluster in the onychophoran Euperipatoides rowelli (Peripatopsidae). BMC Dev. Biol. (IF 2.0) Pub Date : 2019-04-15 Sandra Treffkorn,Georg Mayer
BACKGROUND NK genes are a group of homeobox transcription factors that are involved in various molecular pathways across bilaterians. They are typically divided into two subgroups, the NK cluster (NKC) and NK-linked genes (NKL). While the NKC genes have been studied in various bilaterians, corresponding data of many NKL genes are missing to date. To further investigate the ancestral roles of NK family
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Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-04-05 Karolina Pociute,Jennifer A Schumacher,Saulius Sumanas
BACKGROUND C-lectin family 14 Member A (Clec14a) is a transmembrane protein specifically expressed in vascular endothelial cells during embryogenesis. Previous in vitro and in vivo studies have provided conflicting data regarding Clec14a role in promoting or inhibiting angiogenesis, therefore its functional role in vascular development remains poorly understood. RESULTS Here we have generated a novel
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Differential roles of insulin like growth factor 1 receptor and insulin receptor during embryonic heart development. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-03-25 Kai Wang,Hua Shen,Peiheng Gan,Susana Cavallero,S Ram Kumar,Ching-Ling Lien,Henry M Sucov
BACKGROUND The embryonic day E10-13 period of mouse heart development is characterized by robust cardiomyocyte proliferation that creates the compact zone of thickened ventricular wall myocardium. This process is initiated by the formation of the epicardium on the outer heart surface, which releases insulin-like growth factor 2 (IGF2) as the primary cardiomyocyte mitogen. Two receptors mediate IGF2
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Vitellogenins in the spider Parasteatoda tepidariorum - expression profile and putative hormonal regulation of vitellogenesis. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-03-08 Agata W Bednarek,Marta K Sawadro,Łukasz Nicewicz,Agnieszka I Babczyńska
BACKGROUND Knowledge about vitellogenesis in spiders is rudimentary. Therefore, the aim of study was to check the vitellogenin (Vg) presence in various tissues of the female spider Parasteatoda tepidariorum, determine when and where vitellogenesis starts and takes place, and the putative role of selected hormones in the vitellogenesis. RESULTS Here we show two genes encoding Vg (PtVg4 and PtVg6) in
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Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-03-07 Livia Goto-Silva,Nadia M E Ayad,Iasmin L Herzog,Nilton P Silva,Bernard Lamien,Helcio R B Orlande,Annie da Costa Souza,Sidarta Ribeiro,Michele Martins,Gilberto B Domont,Magno Junqueira,Fernanda Tovar-Moll,Stevens K Rehen
BACKGROUND Organoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems for organoid cultivation have been proposed, but whether they meet similar shear stress parameters as the regularly used spinner flask and its correlation with the successful generation of brain organoids has not been determined
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Interleukin-6 increases inner cell mass numbers in bovine embryos. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-02-01 Lydia K Wooldridge,Alan D Ealy
BACKGROUND Work in other species suggests that interleukin-6 (IL6) promotes early embryo development. It was unclear whether IL6 serves as an embryokine in cultured bovine embryos. This work was undertaken to elucidate the role of IL6 during in vitro bovine embryo production. RESULTS Transcripts for IL6 and its two cognate receptor subunits (IL6R, IL6ST) were confirmed in bovine embryos from the 1-cell
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Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components. BMC Dev. Biol. (IF 2.0) Pub Date : 2019-01-22 Scott J Neal,Darin Dolezal,Nisveta Jusić,Francesca Pignoni
BACKGROUND BMP signaling is involved in myriad metazoan developmental processes, and study of this pathway in Drosophila has contributed greatly to our understanding of its molecular and genetic mechanisms. These studies have benefited not only from Drosophila's advanced genetic tools, but from complimentary in vitro culture systems. However, the commonly-used S2 cell line is not intrinsically sensitive
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Environmental cycles regulate development time via circadian clock mediated gating of adult emergence. BMC Dev. Biol. (IF 2.0) Pub Date : 2018-12-22 Manishi Srivastava,Anjana James,Vishwanath Varma,Vijay Kumar Sharma,Vasu Sheeba
BACKGROUND Previous studies have implicated a role for circadian clocks in regulating pre-adult development of organisms. Among them two approaches are most notable: 1) use of insects whose clocks have different free-running periods and 2) imposition of artificial selection on either rate of development, timing of emergence or circadian period in laboratory populations. Using these two approaches,
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Histone acetyltransferase CBP-related H3K23 acetylation contributes to courtship learning in Drosophila. BMC Dev. Biol. (IF 2.0) Pub Date : 2018-11-20 Kai-Le Li,Lei Zhang,Xiao-Mei Yang,Qiang Fang,Xue-Fang Yin,Hui-Min Wei,Ting Zhou,Ya-Bin Li,Xue-Lin Chen,Fan Tang,Yong-Hao Li,Jian-Feng Chang,Wei Li,Feng Sun
BACKGROUND Histone modifications are critical in regulating neuronal processes. However, the impacts of individual histone modifications on learning and memory are elusive. Here, we investigated the contributions of histone H3 lysine modifications to learning and memory in Drosophila by using histone lysine-to-alanine mutants. RESULTS Behavioural analysis indicated that compared to the H3WT group,
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Fermitin family homolog-2 (FERMT2) is highly expressed in human placental villi and modulates trophoblast invasion. BMC Dev. Biol. (IF 2.0) Pub Date : 2018-11-01 Eiko Kawamura,Gina B Hamilton,Ewa I Miskiewicz,Daniel J MacPhee
BACKGROUND Integrins are transmembrane receptors that mediate cell-extracellular matrix (ECM) and cell-cell adhesion and trophoblast cells undergo changes in integrin expression as they differentiate. However, the mechanism(s) of integrin activation leading to integrin-mediated signaling in trophoblast cell differentiation is unknown. The Fermitin family proteins are integrin activators that help mediate
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Short term optical defocus perturbs normal developmental shifts in retina/RPE protein abundance. BMC Dev. Biol. (IF 2.0) Pub Date : 2018-08-29 Nina Riddell,Pierre Faou,Sheila G Crewther
BACKGROUND Myopia (short-sightedness) affects approximately 1.4 billion people worldwide, and prevalence is increasing. Animal models induced by defocusing lenses show striking similarity with human myopia in terms of morphology and the implicated genetic pathways. Less is known about proteome changes in animals. Thus, the present study aimed to improve understanding of protein pathway responses to
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Caspases and matrix metalloproteases facilitate collective behavior of non-neural ectoderm after hindbrain neuropore closure. BMC Dev. Biol. (IF 2.0) Pub Date : 2018-07-31 Naomi Shinotsuka,Yoshifumi Yamaguchi,Kenichi Nakazato,Yudai Matsumoto,Atsushi Mochizuki,Masayuki Miura
BACKGROUND Mammalian brain is formed through neural tube closure (NTC), wherein both ridges of opposing neural folds are fused in the midline and remodeled in the roof plate of the neural tube and overlying non-neural ectodermal layer. Apoptosis is widely observed from the beginning of NTC at the neural ridges and is crucial for the proper progression of NTC, but its role after the closure remains