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Epigenetic modification of histone acetylation in the sensorimotor cortex after intracerebral hemorrhage Biomed. Res. (IF 1.2) Pub Date : 2024-02-06 Taichi NISHIO, Takahiro INOUE, Yasuyuki TAKAMATSU, Taiga MISHIMA, Hana TAKAMURA, Kiho SOMA, Yuki KONDO, Misato OKAMURA, Ryo IKEGAMI, Hiroshi MAEJIMA
Epigenetic regulation is involved in post-stroke neuroplasticity. We investigated the effects of intracerebral hemorrhage (ICH) on histone acetylation and gene expression related to neuronal plasticity in the bilateral sensorimotor cortices, which may affect post-stroke sensorimotor function. Wistar rats were randomly divided into the SHAM and ICH groups. We performed ICH surgery stereotaxically based
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Rutin promotes M2 phenotype microglia polarization by suppressing the JAK/STAT3 signaling to protect against retinal ischemia-reperfusion injury Biomed. Res. (IF 1.2) Pub Date : 2024-02-06 An-Le SU, Shuai ZHAO, Hong-Na ZHU, Ying QIAO, Ting ZHANG
We aimed to investigate the neuroprotective effect of rutin on retinal ganglion cells (RGCs) under ischemia-reperfusion (I/R) conditions and the underlying mechanisms involving microglia polarization and JAK/STAT3 signaling. RGCs isolated from C57/Bl6 mice were co-cultured with BV2 microglial cells under normal or in vitro oxygen-glucose deprivation and reoxygenation (OGD/R) conditions. Rutin’s effects
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Cell cycle dependence of cell survival following exposure to X-rays in synchronous HeLa cells expressing fluorescent ubiquitination-based cell cycle indicators Biomed. Res. (IF 1.2) Pub Date : 2024-02-06 Ryosuke SEINO, Hiroto UNO, Kevin M PRISE, Hisanori FUKUNAGA
The cell cycle dependence of radiosensitivity has yet to be fully determined, as it is technically difficult to achieve a high degree of cell cycle synchronization in cultured cell systems and accurately detect the cell cycle phase of individual cells simultaneously. We used human cervical carcinoma HeLa cells expressing fluorescent ubiquitination-based cell cycle indicators (FUCCI), and employed the
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Fish oil consumption prevents hepatic lipid accumulation induced by high-cholesterol feeding in obese KK mice Biomed. Res. (IF 1.2) Pub Date : 2024-02-06 Satoshi HIRAKO, HyounJu KIM, Yuzuru IIZUKA, Akiyo MATSUMOTO
Fish oil (FO) is rich in the n-3 polyunsaturated fatty acids. It has been demonstrated that FO intake possesses lipid-lowering properties. Conversely, a high-cholesterol (CH) diet promotes lipid accumulation in the liver and induces fatty liver. This study investigated the effects of FO feeding on hepatic lipid accumulation induced by high-cholesterol feeding in KK mice. All experimental diets had
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Involvement of interaction of Cav3.2 and nociceptive TRPA1 in pathological pain transmission Biomed. Res. (IF 1.2) Pub Date : 2024-02-06 Minami NAKAGAWA, Kenji TAKAHASHI, Yuki NISHIZAWA, Toshio OHTA
T-type Ca2+ channels and TRPA1 expressed in sensory neurons are involved in pain. We previously demonstrated a functional interaction of these channels under physiological conditions. Here we investigated the possible involvement of these channels in inflammatory pain condition. We also evaluated the relationship of these channels endogenously expressed in RIN-14B, a rat pancreatic islet tumor cell
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GP2-expressing cells: a new guardian with divergent functions in the intestine, eyes, and nose Biomed. Res. (IF 1.2) Pub Date : 2023-11-24 Toshihiko IWANAGA, Shunsuke KIMURA
GP (glycoprotein)-2, originally identified as a predominant membranous component of pancreatic acinar cells, has attracted the interest of researchers in mucosal immunology for its role as a functional molecule specific for antigen-sampling cells in the intestinal Peyer’s patches. GP2 is involved in the detection of pathological bacteria and is also histologically useful for the identification of the
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Hypoxia-induced endothelial cell-derived exosome stimulates vascular smooth muscle cell proliferation and migration Biomed. Res. (IF 1.2) Pub Date : 2023-11-24 Qinggen XIONG, Fei LU, Xiaoming XIE, Wei ZHOU
This study mainly used human VSMCs and ECs cultured in vitro to investigate whether exosomes (Exos) are involved in the communication between ECs and VSMCs under hypoxia, and to explore the role and mechanism of ECs-derived exosomes in the abnormal proliferation of VSMCs. VSMCs proliferation and migration were assessed by a series of cell function assays after culturing VSMCs alone or co-culturing
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Fractalkine’s dual role in inflammation and hard tissue formation in cultured human dental pulp cells Biomed. Res. (IF 1.2) Pub Date : 2023-11-24 Natsuko GOMYO-FURUYA, Naoto KAMIO, Takahiro WATANABE, Tomomi HAYAMA, Joji FUKAI, Kosei KURAMOCHI, Kento NAKANISHI, Arata WATANABE, Tatsu OKABE, Kiyoshi MATSUSHIMA
This study aimed to explore the potential roles of fractalkine/CX3CR1, primarily expressed in vascular endothelial cells and has recently been identified in dental pulp cells at sites of pulp tissue inflammation, not only in inflammation but also in pulp hard tissue formation. To this end, cultured human dental pulp cells were grown in 10% FBS-supplemented α-MEM. Fractalkine was introduced to the culture
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Validation of mitotic harvesting method with human cervical carcinoma HeLa cells expressing fluorescent ubiquitination-based cell cycle indicators for radiation research Biomed. Res. (IF 1.2) Pub Date : 2023-09-30 Ryosuke SEINO, Hiroto UNO, Hisanori FUKUNAGA
The cell cycle is a series of events in the process of one cell giving rise to two daughter cells. The mitotic harvesting method, established by Terasima and Tolmach in the 1960s, causes minimal physiological stress on the cells and achieves a high degree of cell cycle synchrony by collecting only mitotic cells from a cultured cell system. The purpose of the present study is to validate the versatility
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Impact of somatic mutations and transcriptomic alterations on cancer aneuploidy Biomed. Res. (IF 1.2) Pub Date : 2023-09-30 Keiichi HATAKEYAMA, Takeshi NAGASHIMA, Keiichi OHSHIMA, Sumiko OHNAMI, Shumpei OHNAMI, Yuji SHIMODA, Akane NARUOKA, Koji MARUYAMA, Akira IIZUKA, Tadashi ASHIZAWA, Hirotsugu KENMOTSU, Tohru MOCHIZUKI, Kenichi URAKAMI, Yasuto AKIYAMA, Ken YAMAGUCHI
Aneuploidy has been recognized as one of hallmark of tumorigenesis since the early 20th century. Recent developments in structural variation analysis in the human genome have revealed the diversity of aneuploidy in cancer. However, the effects of gene mutation and expression in tumors on aneuploidy remain poorly understood. Here, we performed whole exome analysis of over 5,000 Japanese cancer cases
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Protective effects of hachimijiogan (HJG), a Japanese Kampo medicine, on cancer cachectic muscle wasting in mice Biomed. Res. (IF 1.2) Pub Date : 2023-09-30 Satoshi KAMETAKA, Mari ISOBE, Kenshin KOMATA, Makoto MORINAGA, Kazuma NAGAHATA, Sachiko Lee-HOTTA, Yasushi UCHIYAMA, Masahiro SHIBATA, Hideshi SUGIURA
Myogenesis is required to generate skeletal muscle tissue and to maintain skeletal muscle mass. Decreased myogenesis under various pathogenic conditions results in muscular atrophy. Through a small screening of Japanese traditional (Kampo) medicines, hachimijiogan (HJG) was shown to promote the myogenic differentiation of C2C12 myoblasts through the upregulation of myogenin. In tumor-bearing cancer-cachectic
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Acute effect of electrical stimulation on muscle protein synthesis and break-down in the soleus muscle of hindlimb unloaded rats Biomed. Res. (IF 1.2) Pub Date : 2023-09-30 Miho KANAZASHI, Masayuki TANAKA
Electrical stimulation (ES) is effective for disuse-induced muscle atrophy. However, the acute effect of ES on muscle protein synthesis (MPS) and muscle protein breakdown (MPB) remains unclear. We investigated the effect of a single-session ES treatment on mTORC1 signaling, MPS, and MPB in the soleus muscle of 2-week hindlimb unloaded rats. Sprague Dawley rats (n = 12 male) were randomly divided into
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3-Iodothyronamine, a trace amine-associated receptor agonist, regulates intracellular Ca2+ increases via CaMK II through Epac2 in rat cerebral arterioles Biomed. Res. (IF 1.2) Pub Date : 2023-09-30 Wakana SAKANOUE, Takuya YOKOYAMA, Masato HIRAKAWA, Satsuki MAESAWA, Kenichi SATO, Tomoyuki SAINO
Trace amines (TAs) in the nervous system bind to TA-associated receptors (TAARs) and are involved in the regulation of monoaminergic functions. Among TAAR subtypes, TAAR1 has been implicated in the development of neurological disorders, such as schizophrenia. The present study investigated the effects of the TAAR1 agonist, 3-iodothyronamine (T1AM) on cerebral arterioles using fluctuations in the intracellular
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Comparative analysis of tumor content estimation methods based on simu- lated tumor samples identified their impact on somatic variant detection in cancer whole genome sequencing Biomed. Res. (IF 1.2) Pub Date : 2023-08-05 Takeshi NAGASHIMA, Kenichi URAKAMI, Yuji SHIMODA, Keiichi OHSHIMA, Masakuni SERIZAWA, Keiichi HATAKEYAMA, Sumiko OHNAMI, Shumpei OHNAMI, Akane NARUOKA, Yasue HORIUCHI, Akira IIZUKA, Koji MARUYAMA, Yasuto AKIYAMA, Ken YAMAGUCHI
Whole genome sequencing (WGS) in cancer genomics has become widespread with recent technological innovations, and the amount and types of information obtained from WGS are increasing rapidly. Appropriate interpretation of results is becoming increasingly important in clinical applications. This study aimed to evaluate the accuracy of tumor content estimation and its impact on somatic variant detection
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A point of view on human fat olfaction — do fatty derivatives serve as cues for awareness of dietary fats? Biomed. Res. (IF 1.2) Pub Date : 2023-08-05 Satoshi TSUZUKI
Fat (triglycerides) consumption is critical for the survival of animals, including humans. Being able to smell fat can be advantageous in judging food value. However, fat has poor volatility; thus, olfaction of fat seems impossible. What about fatty acids that comprise fat? Humans smell and discriminate medium-chain fatty acids. However, no conclusive evidence has been provided for the olfactory sense
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Neonatal bilateral whisker trimming in male mice age-dependently alters brain neurotransmitter levels and causes adolescent onsets of social behavior abnormalities Biomed. Res. (IF 1.2) Pub Date : 2023-08-05 Hiroyasu MURASAWA, Hitomi SOUMIYA, Hiroyuki KOBAYASHI, Jun IMAI, Takahiko NAGASE, Hidefumi FUKUMITSU
Tactile perception via whiskers is important in rodent behavior. Whisker trimming during the neonatal period affects mouse behaviors related to both whisker-based tactile cognition and social performance. However, the molecular basis of these phenomena is not completely understood. To solve this issue, we investigated developmental changes in transmitters and metabolites in various brain regions of
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Visualization of the localization of phospholipids in developing rat teeth by matrix-assisted laser desorption/ionization imaging mass spectrometry Biomed. Res. (IF 1.2) Pub Date : 2023-08-05 Yasuyuki SASANO, Alu KONNO, Megumi NAKAMURA, Akiko HENMI, Miyuki MAYANAGI, Mu-Chen YANG, Ikuko YAO
Matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS) is used to comprehensively visualize the spatial distribution of numerous biomolecules. The present study was designed to investigate the distribution of phospholipids in developing rat teeth by IMS to identify the characteristic phospholipid molecules for tooth development, and to evaluate the suitability of tissue preparation
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The effect of the formalin-fixed paraffin-embedded process on salivary microbiota profiling Biomed. Res. (IF 1.2) Pub Date : 2023-05-30 Hiroto SANO, Takuichi SATO, Yoriaki KANRI, Junya ONO, Yasuo OKADA
In recent years, bacterial DNA in formalin-fixed paraffin-embedded (FFPE) samples has been recognized as a valuable bioresource for microbiota studies. This study aimed to examine the effect of the FFPE process on microbiota profiling to evaluate whether FFPE samples could serve as an alternative bioresource to fresh samples in oral microbiota studies. Fresh saliva was collected from nine subjects
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Anatomical basis of gastrin- and CCK-secreting cells and their functions. A review Biomed. Res. (IF 1.2) Pub Date : 2023-05-30 Toshihiko IWANAGA
Gastrin and CCK (cholecystokinin), gut hormones first secreted after postprandial stages, share the C-terminal amino acids and some types of receptors to be stimulated. Both types of hormone-secreting cells are typical open-type cells which detect foods and their digested elements in the lumen and regulate the secretion of gastric acid and digestive enzymes, gut motility, and satiety. Gastrin cell
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Red foxtail millet upregulates brain-derived neurotrophic factor levels in vitro and in vivo Biomed. Res. (IF 1.2) Pub Date : 2023-05-30 Kensuke NAKAJIMA, Hayato TOMOHIRO, Shigeru OISO
Upregulation of the brain-derived neurotrophic factor (BDNF) in the brain can help in the prevention and treatment of depression. BDNF is synthesized in various peripheral tissues, as well as in the brain, and can reach the brain via the blood–brain barrier. Therefore, foods that upregulate peripheral BDNF levels may aid in depression management. We previously showed the BDNF-upregulating effect of
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The lncRNA ADAMTS9-AS1/miR-185-5p/KAT7 ceRNA network inhibits cardiomyocyte hypertrophy in hypertrophic obstructive cardiomyopathy Biomed. Res. (IF 1.2) Pub Date : 2023-05-30 Bangrong SONG, Wei LI, Xiaoyu XU, Haiming DANG, Ran DONG
Hypertrophic obstructive cardiomyopathy (HOCM) is a well-recognized inherited cardiac disease. This study was conducted to explore the role of lncRNA ADAMTS9 antisense RNA 1 (ADAMTS9-AS1) in HOCM-induced cardiomyocyte hypertrophy. The serum of HOCM patients was collected. AC16 cells were treated with isoproterenol (ISO) and transfected with oe-ADAMTS9-AS1 vector, miR-185-5p mimic, and lysine acetyltransferase
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Immunohistochemical characterization of the mandibular condyle for type I and II collagen, aggrecan, MMP-9, and MMP-13 in MMP-2-deficient mice Biomed. Res. (IF 1.2) Pub Date : 2023-04-02 Mu-Chen YANG, Megumi NAKAMURA, Miyuki MAYANAGI, Yasuyuki SASANO
Mice devoid of matrix metalloproteinase (MMP)-2 due to gene targeting have been reported to show articular cartilage destruction in the knee joint; however, the phenotype of the mandibular condylar cartilage remains unknown. Thus, in the present study, we investigated the mandibular condyle in Mmp2−/− mice. We obtained and bred Mmp2−/− mice from the same source as the previous study, and performed
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Cannabinoid receptors involved in descending inhibition on spinal seizure-like activity in the phrenic output Biomed. Res. (IF 1.2) Pub Date : 2023-04-02 Shih Tien LIN, Makito IIZUKA, Yoshihiro MIKAMI, Shunya YODA, Hiroshi ONIMARU, Masahiko IZUMIZAKI
Seizure-like burst activities are induced by blockade of GABAA and/or glycine receptors in various spinal ventral roots of brainstem-spinal cord preparation from neonatal rodents. We found that this is not applicable to the phrenic nerve and that a new inhibitory descending pathway may suppress seizure-like activity in the phrenic nerve. Experiments were performed in brainstem-spinal cord preparation
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Role of aquaporin 5 and glandular blood flow in the acetylcholine-induced secretion of saliva in rats Biomed. Res. (IF 1.2) Pub Date : 2023-04-02 MST Tahmina AKTER, Akihiro NEZU, Tetsuya AKAMATSU, Akihiko TANIMURA
To clarify the role of the aquaporin 5 (AQP5) in salivary secretion, we evaluated acetylcholine (ACh)-induced secretion in Sprague-Dawley (SD) rats, rats expressing a low level of AQP5 protein (AQP5/low SD) which developed from SD rats, and Wistar/ST rats. The salivary secretion in AQP5/low SD rats in response to infusions of low-dose ACh (60–120 nmol/min) was 27–42% of that in SD rats. By contrast
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Sasa veitchii extracts protect phenytoin-induced cell proliferation inhibition in human lip mesenchymal cells through modulation of miR-27b-5p Biomed. Res. (IF 1.2) Pub Date : 2023-04-02 Yosuke TSUKIBOSHI, Aya OGATA, Azumi NOGUCHI, Yurie MIKAMI, Satoshi YOKOTA, Kenichi OGATA, Hiroki YOSHIOKA
A cleft lip, with or without a cleft palate, is a common birth defect caused by environmental factors or genetic mutations. Environmental factors, such as pharmaceutical exposure in pregnant women, are known to induce cleft lip, with or without cleft palate in the child. This study aimed to investigate the protective effect of Sasa veitchii extract (SE) on phenytoin-induced inhibition of cell proliferation
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GPRC5B (G protein-coupled receptor class C group 5 member B) suppresses glucose starvation-induced apoptosis in head-and-neck squamous cell carcinoma Biomed. Res. (IF 1.2) Pub Date : 2023-01-23 Keisuke KANAMORI, Shigeyuki OZAWA, Hiroshi IWABUCHI, Takeharu IKOMA, Kenji SUZUKI, Kae TANAKA, Natsuko YOSHIMURA SAWAI, Takahiro ABE, Yasumasa KATO, Ryu-Ichiro HATA, Masaru KOBAYASHI
G protein-coupled receptor class C group 5 member B (GPRC5B) is involved in extracellular glucose sensing, glucose metabolism, and insulin resistance. Many cancers require glucose at high concentrations to survive and grow. We have investigated the association between tumour GPRC5B expression and the prognosis for patients with cancer, including head-and-neck squamous cell carcinoma (HNSCC), using
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IL-33 deficiency suppresses alveolar bone loss in a ligature-induced periodontitis model Biomed. Res. (IF 1.2) Pub Date : 2023-01-23 Natsuko AIDA, Kazuyoshi TAKEDA, Susumu NAKAE, Hirohisa SAITO, Ko OKUMURA, Toshifumi AZUMA, Tatsukuni OHNO
Interleukin-33 (IL-33) is a member of the IL-1 cytokine family that has been studied primarily in the context of type 2 immune responses. Recent reports suggest that IL-33 also enhances the func- tions of various immune cells and contributes to the development of different inflammatory diseas- es. Interestingly, IL-33 and its receptor ST2 axis exerted either inhibitory or promotional effects on alveolar
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Mumefural prevents insulin resistance and amyloid-beta accumulation in the brain by improving lowered interstitial fluid pH in type 2 diabetes mellitus Biomed. Res. (IF 1.2) Pub Date : 2023-01-23 Shigekuni Hosogi, Atsukazu KUWAHARA, Yuko KUWAHARA, Saori TANAKA, Chikao SHIMAMOTO, Noriko TAGAWA, Ikuo KATO, Kanji YOSHIMOTO, Wataru AOI, Kazuyuki TAKATA, Hiroaki MIYAZAKI, Naomi NIISATO, Yasuhiro TSUBO, Katsumi YAGI, Takashi NAKAHARI, Yoshinori MARUNAKA
The present study tried to clarify if mumefural would prevent hyperglycemia, one of the typical symptoms of type 2 diabetes mellitus (T2DM), since mumefural is an extract from Japanese apricots preventing hyperglycemia. To clarify if mumefural would prevent T2DM pathogenesis, we used Otsuka Long-Evans Tokushima fatty (OLETF) rats, T2DM model. Mumefural diminished hyperglycemia, HOMA-IR and plasma triglyceride
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Allergic inflammation disrupts epithelial electrogenic electrolyte transport through cholinergic regulation in the mouse colon Biomed. Res. (IF 1.2) Pub Date : 2023-01-23 Takeshi YAMAMOTO, Yosuke KATSUKI, Yuya KANAUCHI, Shusaku HAYASHI, Makoto KADOWAKI
Intestinal transport of electrolytes is regulated by the enteric nervous system. Acetylcholine (ACh) is considered the most important neurotransmitter for electrolyte transport in the colon. However, electrolyte transport regulated by ACh is not fully understood in the colon. We investigated the regulation of electrogenic electrolyte transport by cholinergic agonists in the mouse colon by measuring
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Anatomical background of the sensory function in the urethra: involvement of endocrine paraneurons and afferent nerves in divergent urogenital functions. A review Biomed. Res. (IF 1.2) Pub Date : 2022-12-14 Toshihiko IWANAGA, Hiromi TAKAHASHI-IWANAGA
The urethra is ontogenetically derived from the cloaca together with distal parts of the large intestine, and serotonin cells are predominant among dispersed endocrine/paracrine cells in the epithelia of both tissues. Analysis of urethral endocrine cells thus helps us to understand the functions of gut endocrine cells and their communication with the nervous system, due to the fact that the urethra
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Consensus molecular subtyping improves the clinical usefulness of canonical tumor markers for colorectal cancer Biomed. Res. (IF 1.2) Pub Date : 2022-12-14 KAGAWA Hiroyuki, HATAKEYAMA Keiichi, SHIOMI Akio, HINO Hitoshi, MANABE Shoichi, YAMAOKA Yusuke, NAGASHIMA Takeshi, OHSHIMA Keiichi, URAKAMI Kenichi, YAMAGUCHI Ken
Transcriptome-based classification, such as consensus molecular subtyping, is expected to be applied to colorectal cancer (CRC). However, the relationship between molecular profiles and classical tumor markers, which are already used in clinical practice, has not been analyzed in a large cohort and remains unclear. We classified more than 1,500 Japanese patients with CRC based on consensus molecular
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Selective estrogen receptor modulators, acting as agonists of estrogen receptor α in osteoblasts, reduce the TGF-β-induced synthesis of macrophage colony-stimulating factor via inhibition of JNK signaling pathway Biomed. Res. (IF 1.2) Pub Date : 2022-12-14 Tomoyuki Hioki, Rie Matsusima-Nishiwaki, Haruhiko Tokuda, Osamu Kozawa
Selective estrogen receptor modulator (SERM) binds to estrogen receptors (ERs) and acts as both an agonist or an antagonist, depending on the target tissue. Raloxifene and bazedoxifene as SERMs are currently used hormone replacement medicines for postmenopausal osteoporosis. Macrophage colony-stimulating factor (M-CSF) secreted from osteoblasts promotes osteoclastogenesis. We have previously demonstrated
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Secondary bile acid lithocholic acid attenuates neurally evoked ion transport in the rat distal colon Biomed. Res. (IF 1.2) Pub Date : 2022-12-14 Kohei TAKAHASHI, Yuko KUWAHARA, Ikuo KATO, Shinji ASANO, Takaharu KOZAKAI, Yoshinori MARUNAKA, Atsukazu KUWAHARA
The inhibitory action of the secondary bile acid lithocholic acid (LCA) on neurally evoked Cl−/HCO3− secretion was investigated using the Ussing-chambered mucosal-submucosal preparation from the rat distal colon. Electrical field stimulation (EFS) evoked cholinergic and noncholinergic secretory responses in the rat distal colon. The responses were almost completely blocked by TTX (10−6 M) but not atropine
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Structure and barrier functions of the perineurium and its relationship with associated sensory corpuscles: A review Biomed. Res. (IF 1.2) Pub Date : 2022-10-14 Toshihiko IWANAGA, Hiromi TAKAHASHI-IWANAGA, Junko NIO-KOBAYASHI, Satomi EBARA
Peripheral nerves are provided with a blood-nerve barrier which prevents the invasion of harmful substances and pathogens, and also regulates metabolic and ionic homeostasis within nerve fascicles. The barrier functions are attributed to both the concentric layer of flattened cells in the perineurium and blood vessels running in the endoneurium. The perineurial cells develop continuous tight junctions
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Pathological examination of Ym1, a chitinase family protein, in Mesocestoides corti-infected mice Biomed. Res. (IF 1.2) Pub Date : 2022-10-14 Junko NIO-KOBAYASHI, Makoto OWHASHI, Toshihiko IWANAGA
Mammals express a set of chitinase family proteins, comprising chitinases, which can hydrolyze chitin, and chitinase-like proteins without the chitinase activity but possessing chitin-binding properties. They act as endogenous lectins, regulating various physiological/pathological events. Ym1, originally identified as an eosinophil chemotactic factor or a macrophage-derived protein in parasite-infected
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Activation of STAT3 (signal transducer and activator of transcription 3) in synovial tissues from the hip joint in the early stage of rapidly destructive coxopathy Biomed. Res. (IF 1.2) Pub Date : 2022-10-14 Tadashi YASUDA, Shigeo HARA, Shinnosuke YAMASHITA, Sadaki MITSUZAWA, Yoshihiro TSUKAMOTO, Hisataka TAKEUCHI, Satoshi OTA, Eijiro ONISHI
Interleukin-6 signaling activates signal transducer and activator of transcription 3 (STAT3), resulting in matrix metalloproteinase-3 (MMP-3) production. The hip joints with rapidly destructive coxopathy (RDC) show rapid chondrolysis, probably by increased MMP-3. This study aimed to elucidate STAT3 activation in the synovial tissues with joint destruction in the early stage of RDC. Synovial tissues
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CD44 as a pathological marker for the early detection of calcineurin inhibitor-induced nephrotoxicity post kidney transplantation Biomed. Res. (IF 1.2) Pub Date : 2022-10-14 Asako HAYASHI, Takayuki OKAMOTO, Junko NIO-KOBAYASHI, Naoya IWAHARA, Ryota SUZUKI, Yasuhiro UEDA, Toshiyuki TAKAHASHI, Yasuyuki SATO, Toshihiko IWANAGA, Kiyohiko HOTTA
Long-term calcineurin inhibitor (CNI) administration causes irreversible nephrotoxicity. Therefore, early CNI-induced nephrotoxicity detection is necessary for patients who will need long-term CNI administration. There is no pathological indicator for early CNI-induced nephrotoxicity. Here, serial protocol kidney biopsy specimens from five kidney-transplant patients with severe CNI-induced nephrotoxicity
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Serum cell-free DNA concentration as a possible prognostic marker in newly diagnosed diffuse large B-cell lymphoma Biomed. Res. (IF 1.2) Pub Date : 2022-08-18 Yuko SHIROUCHI, Yuko MISHIMA, Tomoko TAKAYAMA, Sayuri MINOWA, Yuko ISHIHARA, Mikako TAMBA, Mitsuhito HIRANO, Naoki ONDA, Kengo TAKEUCHI, Dai MARUYAMA
Cell-free DNA (cfDNA) is a fragment of DNA circulating in the blood, and its concentration is often elevated in cancer patients. To investigate the relationships between serum cfDNA concentration and clinical characteristics, including prognosis, we measured serum cfDNA concentration in 114 newly diagnosed lymphoma patients. The cfDNA concentrations in diffuse large B cell lymphoma (DLBCL) (62.5 ng/mL)
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Novel cell-based system to assay cell-cell fusion during myotube formation Biomed. Res. (IF 1.2) Pub Date : 2022-08-18 Mari ISOBE, Yumika SUZUKI, Hideshi SUGIURA, Masahiro SHIBATA, Yuki OHSAKI, Satoshi KAMETAKA
A live assay tool has been established to uncover the precise molecular mechanisms underlying complex cell fusion events in myoblasts. The novel cell-based assay, HiMy (HiBiT-based myoblast fusion), utilizes a recently developed split-luciferase technology. The assay successfully detected cell fusion in differentiating C2C12 myoblast cultures. This allowed us to measure mixing of the cytoplasm, which
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Development of two 410-cancer-gene panel tests for solid tumors and liquid biopsy based on genome data of 5,143 Japanese cancer patients Biomed. Res. (IF 1.2) Pub Date : 2022-08-18 Yuji SHIMODA, Takeshi NAGASHIMA, Kenichi URAKAMI, Fukumi KAMADA, Sou NAKATANI, Maki MIZUGUCHI, Masakuni SERIZAWA, Keiichi HATAKEYAMA, Keiichi OHSHIMA, Tohru MOCHIZUKI, Sumiko OHNAMI, Shumpei OHNAMI, Takeshi KAWAKAMI, Kentaro YAMAZAKI, Haruyasu MURAKAMI, Hirotsugu KENMOTSU, Akio SHIOMI, Yasuto AKIYAMA, Ken YAMAGUCHI
Next-generation sequencing (NGS) is an integral part of precision medicine, and its power for detecting comprehensive genetic alterations may contribute to treatment decisions for patients with advanced, recurrent, or metastatic cancer. An NGS oncology panel developed in the U.S. and Europe, which targets cancer-related genes, has been approved in Japan, and testing is becoming more widespread in clinical
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Effects of ANP and BNP on the generation of respiratory rhythms in brainstem–spinal cord preparation isolated from newborn rats Biomed. Res. (IF 1.2) Pub Date : 2022-08-18 Shino KATSUKI, Shinichiro OTA, Shunya YODA, Hiroshi ONIMARU, Kenji DOHI, Masahiko IZUMIZAKI
Natriuretic peptides (NPs) are a family of peptide hormones produced in cardiac muscle cells and consist mainly of three types: atrial NP (ANP), B-type (or brain) NP (BNP), and C-type NP. We herein report the effects of ANP and BNP on central respiratory activity in brainstem–spinal cord preparation isolated from newborn rats. Bath application of these peptides (100 nM) induced a weak transient depression
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Early reperfusion with hemoglobin vesicles into tracheal subepithelial capillaries in a mouse tracheal transplant model Biomed. Res. (IF 1.2) Pub Date : 2022-08-18 Hiroto ONOZAWA, Mitsutomo KOHNO, Ryo HASHIMOTO, Kana OIWA, Ryota MASUDA, Masatoshi YAMAGUCHI, Tai HATO, Masazumi WATANABE, Hirohisa HORINOUCHI, Hiromi SAKAI, Koichi KOBAYASHI, Masayuki IWAZAKI
Hemoglobin vesicles (HbVs), liposomes containing concentrated hemoglobin extracted from outdated human red blood cells (RBC), are artificial oxygen carriers with a small particle size. To evaluate the reperfusion of capillaries with HbVs in a tracheal transplant model and compare it with that of RBC. Isogenic mice were used as donors and recipients in a parallel trachea transplant model. Both ends
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Disposal of intestinal apoptotic epithelial cells and their fate via divergent routes Biomed. Res. (IF 1.2) Pub Date : 2022-06-20 Toshihiko IWANAGA, Hiromi TAKAHASHI-IWANAGA
Gut epithelial cells are characterized by rapid, constant cell renewal. The disposal of aging epithelial cells around the villus tips of the small intestine occurs so regularly that it has been regarded as a consequence of well-controlled cell death, designated as apoptosis. However, the notion of live cell extrusion in the intestine has been intensively built among researchers, and the disposal processes
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Shotgun proteomic investigation of methyltransferase and methylation profiles in lipopolysaccharide stimulated RAW264.7 murine macrophages Biomed. Res. (IF 1.2) Pub Date : 2022-06-20 Yumi AIZAWA, Masaru MORI, Tsukasa SUZUKI, Akihiro SAITO, Hirofumi INOUE
Arginine methylation is a common post-translational modification which functions as an epigenetic regulator of transcription and plays a key role in various cell signaling pathways. The methylation of arginine residues is catalyzed by protein arginine methyltransferase (PRMT). However, the expression pattern and underlying mechanism of PRMTs and protein methylation profile in lipopolysaccharide (LPS)-induced
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Ca2+-based neural activity recording for rapidly screening behavioral correlates of the claustrum in freely behaving mice Biomed. Res. (IF 1.2) Pub Date : 2022-06-20 Jing QIN, Wu-shuang HUANG, Hao-ran DU, Chun-qing ZHANG, Peng XIE, Han QIN
The claustrum has been hypothesized to participate in high-order brain functions, but experimental studies to demonstrate these functions are currently lacking. Neural activity recording of the claustrum in freely-behaving animals allows for correlating claustral activities with specific behaviors. However, previously utilized methods for studying the claustrum make it difficult to monitor neural activity
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Meis1 plays roles in cortical development through regulation of cellular proliferative capacity in the embryonic cerebrum Biomed. Res. (IF 1.2) Pub Date : 2022-06-20 Eriko ISOGAI, Kazuhiro OKUMURA, Megumi SAITO, Yurika TOKUNAGA, Yuichi WAKABAYASHI
Meis1 (myeloid ecotropic insertion site 1) is known to be related to embryonic development and cancer. In this study, to analyze the function of Meis1 in neural stem cells, we crossed Meis1fl/fl (Meis1 floxed) mice with Nestin-Cre mice. The results showed that Meis1-conditional knockout mice showed cerebral cortex malformation. The mice had a significantly thinner cortex than wildtype mice. At E14
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Silibinin promotes melanogenesis through the PKA and p38 MAPK signaling pathways in melanoma cells Biomed. Res. (IF 1.2) Pub Date : 2022-04-15 Takuhiro UTO, Tomoe OHTA, Koki KATAYAMA, Yukihiro SHOYAMA
Silibinin is a flavonolignan isolated from milk thistle (Silybum marianum). Silibinin has been reported to possess multiple biological activities; however, its effect on melanogenesis remains unclear. This study investigated the effect of silibinin on melanogenesis in melanoma cells and the associated molecular mechanism. Our findings demonstrated that silibinin markedly increased melanin content in
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Oncostatin M reduces the synthesis of macrophage-colony stimulating factor stimulated by TGF-β via suppression of p44/p42 MAP kinase and JNK in osteoblasts Biomed. Res. (IF 1.2) Pub Date : 2022-04-15 Tomoaki DOI, Tomoyuki HIOKI, Junko TACHI, Kyohei UEDA, Rie MATSUSHIMA-NISHIWAKI, Hiroki IIDA, Shinji OGURA, Osamu KOZAWA, Haruhiko TOKUDA
Bone fracture is an important trauma frequently encountered into emergency medicine as well as orthopedics reflecting an aging society. Oncostatin M, an inflammatory cytokine produced by osteal macrophages, has been considered to play a crucial role in fracture healing. Macrophage colony-stimulating factor (M-CSF) secreted from osteoblasts is essential in osteoclastgenesis, and the secretion is stimulated
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Suncus murinus as a novel model animal that is suitable for elucidating the mechanism of daily torpor Biomed. Res. (IF 1.2) Pub Date : 2022-04-15 Yuuki HORII, Kanako OKADERA, Shingo MIYAWAKI, Takahiko SHIINA, Yasutake SHIMIZU
Torpor, a state of lowered body temperature due to active reduction of the metabolic rate, has potential medical benefits. The aim of this study was to establish a novel laboratory animal that enter torpor without imposing complex conditions. When house musk shrews (Suncus murinus) were kept at an ambient temperature of 24°C, most of the animals did not enter daily torpor. However, when the ambient
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Neonatal isoflurane exposure disturbs granule cell migration in the rat dentate gyrus Biomed. Res. (IF 1.2) Pub Date : 2022-02-17 Yosuke UCHIDA, Toshikazu HASHIMOTO, Hitoshi SAITO, Koichi TAKITA, Yuji MORIMOTO
It has been reported that neonatal isoflurane exposure causes behavioral abnormalities following neurodegeneration in animals and gamma-aminobutyric acid type A (GABAA) receptor activation during the synaptogenesis is considered to be one possible trigger. Additionally, the inhibitory effect of excitatory GABAA receptor signaling on the granule cell (GC) migration in the neonatal rat dentate gyrus
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Diet-related changes of basal lamina fenestrations in the villous epithelium of the rat small intestine: Statistical analysis on scanning electron microscopy Biomed. Res. (IF 1.2) Pub Date : 2022-02-17 Masatoshi SATO, Keisuke MORITA, Rie AZUMI, Yusuke MIZUTANI, Manabu HAYATSU, Tatsuo USHIKI, Shuji TERAI
The epithelial basal lamina of the small intestine has numerous fenestrations for intraepithelial migration of leukocytes. We have reported dynamic changes of fenestrations in dietary conditions. To investigate this phenomenon, we performed statistical analyses using scanning electron microscopy images of the epithelial basal lamina of rat intestinal villi after removal of the villous epithelium by
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RIPK1 is a key factor in black carbon-induced cell death Biomed. Res. (IF 1.2) Pub Date : 2022-02-17 Xianyan XU, Zhaojun XU, Shiyong ZENG, Yuhui OUYANG
Air pollution is associated with increased morbidity and mortality and with cell death at a cellular level. However, the exact mechanism of particulate matter-induced cell death remains to be elucidated. The aim of the present in vitro study using human alveolar epithelial cells (A549) was to determine the cell death pathway(s) induced by black carbon (BC) and ozone oxidized-black carbon (O-BC). BC
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Cellular expression of CD26/dipeptidyl peptidase IV Biomed. Res. (IF 1.2) Pub Date : 2021-12-22 Toshihiko IWANAGA, Junko NIO-KOBAYASHI
Dipeptidyl peptidase 4 (DPP4), a serine protease expressed on luminal and apical cell membrane, is identical to the lymphocyte cell surface protein CD26. DPP4 rapidly deactivates hormones and cytokines by cleaving their NH2-terminal dipeptides. Its functions are based on membrane digestion and/or binding of bioactive peptides, signal molecules, and extracellular matrix components. The soluble form
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Long non-coding RNA TUG1 promotes the osteogenic differentiation of bone marrow mesenchymal stem cells by regulating the AMPK/mTOR/autophagy pathway Biomed. Res. (IF 1.2) Pub Date : 2021-12-22 Ding-gui LU, Mei-jiao LU, Shun-han YAO, Jia-jie LIN, Su LUO, Ji-hua WEI, Yu-jin TANG
Promoting the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts is an effective strategy against osteoporosis. Long non-coding RNAs are closely implicated in BMSC osteogenic differentiation. The present study explored the expression pattern and biological role of taurine upregulated gene 1 (TUG1) in osteogenic differentiation. The expressions of TUG1 and osteogenic markers
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SPERT gene silencing inhibits the growth of human colon cancer xenograft tumor in nude mice via p38MAPK/HSP27 signaling pathway Biomed. Res. (IF 1.2) Pub Date : 2021-12-22 Si-zeng CHEN, Long-zhi ZHENG
Colorectal cancer is one of the most common gastrointestinal malignancies and is also a disease of genetic heterogeneity. Our previous studies have shown that SPERT (sprermatid-associated protein) gene may be an underlying oncogene that is associated with the progression of the disease in colorectal cancer patients, and SPERT gene silencing can inhibit the proliferation of colorectal tumor cells and
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Protective effects of chlorogenic acid on capillary regression caused by disuse muscle atrophy Biomed. Res. (IF 1.2) Pub Date : 2021-12-22 Jihao XING, Han PAN, Hao LIN, Ryosuke NAKANISHI, Takumi HIRABAYASHI, Emi NAKAYAMA, Xiaoqi MA, Noriaki MAESHIGE, Hiroyo KONDO, Hidemi FUJINO
Inactivity causes muscle atrophy and capillary regression in skeletal muscle. Chlorogenic acid has an antioxidant capacity and may prevent capillary regression. Therefore, the protective effects of chlorogenic acid on inactivity-induced capillary regression in rat soleus muscle were investigated. Twenty male Wistar rats were randomly divided into four groups: control (CON), chlorogenic acid supplementation
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Histochemical characteristics on minimodeling-based bone formation induced by anabolic drugs for osteoporotic treatment Biomed. Res. (IF 1.2) Pub Date : 2021-09-21 Tomomaya YAMAMOTO, Tomoka HASEGAWA, Paulo Henrique Luiz de FRAITAS, Hiromi HONGO, Shen ZHAO, Tsuneyuki YAMAMOTO, Alireza NASOORI, Miki ABE, Haruhi MARUOKA, Keisuke KUBOTA, Yasuhito MORIMOTO, Mai HARAGUCHI, Tomohiro SHIMIZU, Masahiko TAKAHATA, Norimasa IWASAKI, Minqi LI, Norio AMIZUKA
Modeling, the changes of bone size and shape, often takes place at the developmental stages, whereas bone remodeling—replacing old bone with new bone—predominantly occurs in adults. Unlike bone remodeling, bone formation induced by modeling i.e., minimodeling (microscopic modeling in cancellous bone) is independent of osteoclastic bone resorption. Although recently-developed drugs for osteoporotic
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Nicotinamide mononucleotide augments the cytotoxic activity of natural killer cells in young and elderly mice Biomed. Res. (IF 1.2) Pub Date : 2021-09-21 Kazuyoshi TAKEDA, Ko OKUMURA
Nicotinamide mononucleotide (NMN), a key nicotinamide adenine dinucleotide (NAD+) intermediate, has been shown to ameliorate various pathologies in elderly mouse disease models. Natural killer (NK) cells are important innate immune cells; however, their functions decline with aging. In this study, we examined the effect of NMN treatment on NK cells in mice. Intraperitoneal administration of NMN augmented
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Assessment of direct binding interaction between CD36 and its potential lipid ligands using a peptide mimic of the receptor labeled with a fluorophore Biomed. Res. (IF 1.2) Pub Date : 2021-09-21 Satoshi TSUZUKI, Yusaku KIMOTO, Masayuki YAMASAKI, Tatsuya SUGAWARA, Yuki MANABE, Kazuo INOUE, Tsutomu SASAKI
Cluster of differentiation 36 (CD36) is a cell-surface receptor that recognizes diverse substances. We have presented indirect evidence that a short segment of the receptor comprising amino acids 149–168 contains a site for binding of its lipid ligands (e.g., distinct fatty acids and aldehydes). However, experimental support for their direct interactions is yet to be achieved. For this, we devised
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Spontaneous calcium responses of SF2 rat dental epithelial cells stably expressing the calcium sensor G-GECO Biomed. Res. (IF 1.2) Pub Date : 2021-09-21 Narumi ISHIDA, Kaori MURATA, Takao MORITA, Shingo SEMBA, Akihiro NEZU, Akihiko TANIMURA
Genetically-encoded calcium indicators such as G-GECO are useful for studying Ca2+ responses during long-term processes. In this study, we employed a lentiviral vector and established a rat dental epithelial cell line that stably expressed G-GECO (SF2-G-GECO). Ca2+ imaging analysis under cell culture conditions revealed that SF2-G-GECO cells exhibited spontaneous Ca2+ responses, which could be classified