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Lipid Nanovesicle Platforms for Hepatocellular Carcinoma Precision Medicine Therapeutics: Progress and Perspectives Organogenesis (IF 2.3) Pub Date : 2024-02-15 Brandon M. Lehrich, Evan R. Delgado
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality globally. HCC is highly heterogenous with diverse etiologies leading to different driver mutations potentiati...
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Regulation of Interferon-β-Modified Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes in Proliferation and Apoptosis of Prostate Cancer Cells Organogenesis (IF 2.3) Pub Date : 2023-11-30 Haichao Yuan, Senmao Li, Zhengping Zhao, Yan Wang
Prostate cancer (PCa) poses a serious burden to men. Interferon-β (IFN-β) is implicated in cancer cell growth. This study hence explored the regulation of IFN-β-modified human umbilical cord mesenc...
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Cell Therapy Approaches for Articular Cartilage Regeneration Organogenesis (IF 2.3) Pub Date : 2023-11-14 Meagan J. Makarczyk
Articular cartilage is a common cartilage type found in a multitude of joints throughout the human body. However, cartilage is limited in its regenerative capacity. A range of methods have been emp...
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Organ-On-A-Chip: An Emerging Research Platform Organogenesis (IF 2.3) Pub Date : 2023-11-15 Nithin R, Ayushi Aggarwal, Anne Boyina Sravani, Pooja Mallya, Shaila Lewis
In drug development, conventional preclinical and clinical testing stages rely on cell cultures and animal experiments, but these methods may fall short of fully representing human biology. To over...
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Human Hepatocellular response in Cholestatic Liver Diseases Organogenesis (IF 2.3) Pub Date : 2023-08-20 Kimberly Ortiz, Zeliha Cetin, Yiyue Sun, Zhiping Hu, Takeshi Kurihara, Edgar N. Tafaleng, Rodrigo M. Florentino, Alina Ostrowska, Alejandro Soto-Gutierrez, Lanuza A.P. Faccioli
ABSTRACT Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC), the most common types of cholestatic liver disease (CLD), result in enterohepatic obstruction, bile acid accumulation, and hepatotoxicity. The mechanisms by which hepatocytes respond to and cope with CLD remain largely unexplored. This study includes the characterization of hepatocytes isolated from explanted livers
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CircHLA-C: A significantly upregulated circRNA co-existing in oral leukoplakia and oral lichen planus Organogenesis (IF 2.3) Pub Date : 2023-08-02 Jingwen Yang, Yuhan Song, Siming Xu, Shuyun Ge, Zhou Haiwen
ABSTRACT Background Oral leukoplakia (OLK) and oral lichen planus (OLP) are common precancerous lesions of the oral mucosa. The role of circular RNAs (circRNAs) in OLK and OLP is unclear. The aim of this study was to evaluate the circRNA expression profiles of OLK and OLP, and further explore the potential role of circRNAs in the pathogenesis of these two diseases. Methods High throughput sequencing
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Full-thickness dermal wound regeneration using hypoxia preconditioned blood-derived growth factors: A case series Organogenesis (IF 2.3) Pub Date : 2023-07-13 Hadjipanayi Ektoras, Moog Philipp, Jiang Jun, Dornseifer Ulf, Machens Hans-Günther, Schilling Arndt F
ABSTRACT Hard-to-heal wounds can be detrimental to patients’ quality of life. Currently, there is scarcity of therapeutic alternatives to mainstay surgical treatment, which uses the principles of tissue debridement, temporary wound coverage, and subsequent tissue reconstruction. Here, a new approach is proposed that harnesses the regenerative power of autologous peripheral blood, through a process
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Expression Levels of WNT Signaling Pathway Genes During Early Tooth Development Organogenesis (IF 2.3) Pub Date : 2023-05-17 Yuhan Song, Fujie Song, Xuan Xiao, Zhifeng Song, Shangfeng Liu
ABSTRACT It is known to all that Wnt signaling pathway plays an important role in the early development of tooth. Our previous research found that Wnt signaling pathway played crucial roles in dental development, and mutations in antagonist of Wnt signaling pathway may lead to the formation of supernumerary teeth. However, the expression pattern of Wnt signaling molecules in early development of tooth
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Adipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal Defects Organogenesis (IF 2.3) Pub Date : 2023-05-15 Makoto Hisanaga, Tomoshi Tsuchiya, Hironosuke Watanabe, Koichiro Shimoyama, Mayumi Iwatake, Yukinori Tanoue, Keizaburo Maruyama, Hiroshi Yukawa, Kazuhide Sato, Yoshimi Kato, Keitaro Matsumoto, Takuro Miyazaki, Ryoichiro Doi, Koichi Tomoshige, Takeshi Nagayasu
ABSTRACT Decellularized scaffolds are promising biomaterials for tissue and organ reconstruction; however, strategies to effectively suppress the host immune responses toward these implants, particularly those without chemical crosslinking, remain warranted. Administration of mesenchymal stem cells is effective against immune-mediated inflammatory disorders. Herein, we investigated the effect of isogeneic
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The Role of Gasdermin-D-Mediated Pryoptosis in Organ Injury and Its Therapeutic Implications Organogenesis (IF 2.3) Pub Date : 2023-02-22 Joud Mulla, Rohan Katti, Melanie J. Scott
ABSTRACT Gasdermin-D (GSDMD) belongs to the Gasdermin family (GSDM), which are pore-forming effector proteins that facilitate inflammatory cell death, also known as pyroptosis. This type of programmed cell death is dependent on inflammatory caspase activation, which cleaves gasdermin-D (GSDMD) to form membrane pores and initiates the release of pro-inflammatory cytokines. Pyroptosis plays an important
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Clinical Manufacturing of Regulatory T Cell Products For Adoptive Cell Therapy and Strategies to Improve Therapeutic Efficacy Organogenesis (IF 2.3) Pub Date : 2023-01-22 Kassandra J. Baron, Hēth R. Turnquist
ABSTRACT Based on successes in preclinical animal transplant models, adoptive cell therapy (ACT) with regulatory T cells (Tregs) is a promising modality to induce allograft tolerance or reduce the use of immunosuppressive drugs to prevent rejection. Extensive work has been done in optimizing the best approach to manufacture Treg cell products for testing in transplant recipients. Collectively, clinical
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Applications of Transcriptomics in the Research of Antibody-Mediated Rejection in Kidney Transplantation: Progress and Perspectives Organogenesis (IF 2.3) Pub Date : 2022-10-19 Hsuan Yeh
ABSTRACT Antibody-mediated rejection (ABMR) is the major cause of chronic allograft dysfunction and loss in kidney transplantation. The immunological mechanisms of ABMR that have been featured in the latest studies indicate a highly complex interplay between various immune and nonimmune cell types. Clinical diagnostic standards have long been criticized for being arbitrary and the lack of accuracy
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Focusing on Hippo Pathway in Stem Cells of Oral Origin, Enamel Formation and Periodontium Regeneration Organogenesis (IF 2.3) Pub Date : 2022-07-04 Tianyi Wang, Kehan Li, Hanghang Liu, En Luo
ABSTRACT Hippo pathway is a cellular regulatory pathway composed of core molecules such as MST1/2, LATS1/2, SAV1, MOB1A/B and downstream YAP/TAZ. Fully involved in regulating cell proliferation, differentiation, migration and apoptosis, the Hippo pathway is critical in regulating stem cells of oral origin, for instance, DPSCs and PDLSCs, enamel formation and periodontium regeneration. Here, we summarized
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A Systematic Approach of the Intrauterine Morphogenesis of the Human Palpebral Apparatus Organogenesis (IF 2.3) Pub Date : 2022-05-22 Octavian Munteanu, Florin-Mihail Filipoiu, Monica Mihaela Cirstoiu, Roxana Elena Bohiltea, Tiberiu Augustin Georgescu, Adrian Dumitru, Andra-Ioana Băloiu, Mihai-Alin Publik, Ioan-Andrei Petrescu
ABSTRACT The human eyelid embodies a vast diversity of functions. Acting as a protective shield for the ocular apparatus and as a light regulator in the sight process, eyelids stand a fascinating – yet omitted – role in facial aesthetics, serving as a racial trait by which humankind succeeded to manifest heterogeneity as a species. These assumptions are precisely forecasted right from in-utero life
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Hepatocyte Differentiation from iPSCs or MSCs in Decellularized Liver Scaffold: Cell–ECM Adhesion, Spatial Distribution, and Hepatocyte Maturation Profile Organogenesis (IF 2.3) Pub Date : 2022-04-17 Radiana Dhewayani Antarianto, Adrian Pragiwaksana, Wahyunia Likhayati Septiana, Nuzli Fahdia Mazfufah, Ameer Mahmood
ABSTRACT Mesenchymal stem cells (MSC) and induced pluripotent stem cells (iPSC) have been reported to be able to differentiate to hepatocyte in vitro with varying degree of hepatocyte maturation. A simple method to decellularize liver scaffold has been established by the Department of Histology, Faculty of Medicine, Universitas Indonesia, in SCTE IMERI lab.15 This study aims to evaluate hepatocyte
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Variations in Aspects of Neural Precursor Cell Neurogenesis in a Human Model of HSV-1 Infection. Organogenesis (IF 2.3) Pub Date : 2022-12-31 Wenxiao Zheng,Emily M Benner,David C Bloom,Vaishali Muralidaran,Jill K Caldwell,Anuya Prabhudesai,Paolo A Piazza,Joel Wood,Paul R Kinchington,Vishwajit L Nimgaonkar,Leonardo D'Aiuto
Encephalitis, the most significant of the central nervous system (CNS) diseases caused by Herpes simplex virus 1 (HSV-1), may have long-term sequelae in survivors treated with acyclovir, the cause of which is unclear. HSV-1 exhibits a tropism toward neurogenic niches in CNS enriched with neural precursor cells (NPCs), which play a pivotal role in neurogenesis. NPCs are susceptible to HSV-1. There is
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Upregulation of miR-128 Mediates Heart Injury by Activating Wnt/β-catenin Signaling Pathway in Heart Failure Mice Organogenesis (IF 2.3) Pub Date : 2021-12-29 Jing-Yao Li, Xin-Chang Li, Yu-Long Tang
ABSTRACT Cardiac hypertrophy contributes to heart failure and is pathogenically modulated by a network of signaling cascades including Wnt/β-catenin signaling pathway. miRNAs have been widely demonstrated to regulate gene expression in heart development. miR-128 was routinely found as a brain-enriched gene and has been functionally associated with regulation of cardiac function. However, its role and
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Human Hepatocytes Isolated from Explanted Livers: A Powerful Tool to Understand End-stage Liver Disease and Drug Screening Organogenesis (IF 2.3) Pub Date : 2022-02-03 Lanuza A. P. Faccioli, Zehra N. Kocas-Kilicarslan, Ricardo Diaz-Aragon, Takashi Motomura, Sriram Amirneni, Michelle R. Malizio, Michael C. Coard, Carla Frau, Nils Haep, Rodrigo M. Florentino, Alina Ostrowska
ABSTRACT The use of primary human hepatocytes has been hampered by limited availability of adequate numbers of fresh and viable cells due to the ongoing shortage of liver donors. Thus, there is no surplus of healthy organs from which freshly isolated cells can be prepared when needed. However, primary hepatocytes can be successfully isolated from explanted liver specimens obtained from patients receiving
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Therapeutic Potential of HNF4α in End-stage Liver Disease Organogenesis (IF 2.3) Pub Date : 2022-02-03 Ricardo Diaz-Aragon, Michael C. Coard, Sriram Amirneni, Lanuza Faccioli, Nils Haep, Michelle R. Malizio, Takashi Motomura, Zehra N. Kocas-Kilicarslan, Alina Ostrowska, Rodrigo M. Florentino, Carla Frau
ABSTRACT The prevalence of end-stage liver disease (ESLD) in the US is increasing at an alarming rate. It can be caused by several factors; however, one of the most common routes begins with nonalcoholic fatty liver disease (NAFLD). ESLD is diagnosed by the presence of irreversible damage to the liver. Currently, the only definitive treatment for ESLD is orthotopic liver transplantation (OLT). Nevertheless
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Evaluation of the Growth and Differentiation of Human Fetal Osteoblasts (hFOB) Cells on Demineralized Bone Matrix (DBM) Organogenesis (IF 2.3) Pub Date : 2021-11-30 Flavia Oliveira Pinho, Paulo Pinto Joazeiro, Arnaldo R. Santos Jr
ABSTRACT Cells with osteogenic potential are believed to be an ideal source for bone tissue bioengineering. Large bone defects require temporary substitution of the damaged parts. In this respect, the transplantation of bone cells cultured on osteogenic substrates has been investigated. To use the natural bone matrix, one approach is the so-called demineralized bone matrix (DBM). In this study, we
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The Genes Involved in Dentinogenesis Organogenesis (IF 2.3) Pub Date : 2022-01-13 Shuang Chen, Han Xie, Shouliang Zhao, Shuai Wang, Xiaoling Wei, Shangfeng Liu
ABSTRACT The development and repair of dentin are strictly regulated by hundreds of genes. Abnormal dentin development is directly caused by gene mutations and dysregulation. Understanding and mastering this signal network is of great significance to the study of tooth development, tissue regeneration, aging, and repair and the treatment of dental diseases. It is necessary to understand the formation
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Time-dependently Appeared Microenvironmental Changes and Mechanism after Cartilage or Joint Damage and the Influences on Cartilage Regeneration Organogenesis (IF 2.3) Pub Date : 2021-11-22 Danyang Yue, Lin Du, Bingbing Zhang, Huan Wu, Qiong Yang, Min Wang, Jun Pan
ABSTRACT Cartilage and joint damage easily degenerates cartilage and turns into osteoarthritis (OA), which seriously affects human life and work, and has no cure currently. The temporal and spatial changes of multiple microenvironments upon the damage of cartilage and joint are noticed, including the emergences of inflammation, bone remodeling, blood vessels, and nerves, as well as alterations of extracellular
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Mimicked Periosteum Layer Based on Deposited Particle Silk Fibroin Membrane for Osteogenesis and Guided Bone Regeneration in Alveolar Cleft Surgery: Formation and in Vitro Testing Organogenesis (IF 2.3) Pub Date : 2021-11-01 Yadanar Mya Moe, Thongchai Nuntanaranont, Matthana Khangkhamano, Jirut Meesane
ABSTRACT An alveolar cleft is a critical tissue defect often treated with surgery. In this research, the mimicked periosteum layer based on deposited silk fibroin membrane was fabricated for guided bone regeneration in alveolar cleft surgery. The deposited silk fibroin particle membranes were fabricated by spray-drying with different concentrations of silk fibroin (v/v): 0.5% silk fibroin (0.5% SFM)
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Connection of ES Cell-derived Collecting Ducts and Ureter-like Structures to Host Kidneys in Culture Organogenesis (IF 2.3) Pub Date : 2021-09-27 May Sallam, Jamie Davies
ABSTRACT Work toward renal generation generally aims either to introduce suspensions of stem cells into kidneys in the hope that they will rebuild damaged tissue, or to construct complete new kidneys from stem cells with the aim of transplanting the engineered organs. In principle, there might be a third approach; to engineer renal tissue ‘modules’ in vitro and to use them to replace sections of damaged
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A New View of Bone Loss in Phenylketonuria Organogenesis (IF 2.3) Pub Date : 2021-08-25 Steven F. Dobrowolski, Irina L. Tourkova, Cayla R. Sudano, Quitterie C Larrouture, Harry C. Blair
ABSTRACT Osteopenia is common in phenylalanine hydroxylase deficient phenylketonuria (PKU). PKU is managed by limiting dietary phenylalanine. Osteopenia in PKU might reflect a therapeutic diet, with reduced bone forming materials. However, osteopenia occurs in patients who never received dietary therapy or following short-term therapy. Humans and animal studies find no correlation between bone loss
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Potential of Autologous Progenitor Cells and Decellularized Porcine Artery Matrix in Construction of Tissue-engineered Vascular Grafts Organogenesis (IF 2.3) Pub Date : 2021-08-18 Jieh-Neng Wang, Chung-Dann Kan, Shao-Hsien Lin, Ko-Chi Chang, Stephanie Tsao, Tak-Wah Wong
ABSTRACT To develop a tissue-engineered vascular graft, we used pericardial effusion-derived progenitor cells (PEPCs) collected from drained fluid after open-heart surgery in children with congenital heart diseases to repopulate a decellularized porcine pulmonary artery. The PEPCs were compared with human fibroblasts (HS68) and human umbilical vein endothelial cells (HUVECs) in cell growth and migration
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Osteogenic and Angiogenic Synergy of Human Adipose Stem Cells and Human Umbilical Vein Endothelial Cells Cocultured in a Modified Perfusion Bioreactor Organogenesis (IF 2.3) Pub Date : 2021-07-29 Fatemeh Mokhtari-Jafari, Ghasem Amoabediny, Mohammad Mehdi Dehghan, Sonia Abbasi Ravasjani, Massoumeh Jabbari Fakhr, Yasaman Zamani
ABSTRACT Synergistic promotion of angiogenesis and osteogenesis in bone tissue-engineered constructs remains a crucial clinical challenge, which might be overcome by simultaneous employment of superior techniques including coculture systems, differentiation-stimulated factors, combinatorial scaffolds and bioreactors. Current study investigated the effect of flow perfusion along with coculture of human
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A Comprehensive Study Regarding the Intrauterine Development of Nails Organogenesis (IF 2.3) Pub Date : 2021-03-30 Octavian Munteanu, Florin-Mihail Filipoiu, Monica Mihaela Cirstoiu, Andra-Ioana Băloiu, Ioan-Andrei Petrescu, Roxana Elena Bohiltea
ABSTRACT The nail apparatus serves as a protective layer over the dorsal aspect of each distal phalanx of both hands and feet. Besides protection, other functions include serving as part of defense or attack mechanisms, scratching, and dexterity. Nail development has been a subject of interest since the 19th century, from both the phylogenetic and ontogenetic points of view. Despite the early spark
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Persistent Labial Minora Fusion in Reproductive Age Women: A Retrospective Case Series of Nine Patients and Review of Literature Organogenesis (IF 2.3) Pub Date : 2021-05-20 Ze Liang, Juan Chen, Xin Yu, Lan Zhu
ABSTRACT Labial minora fusion persisting to the reproductive age is a rare type of labial fusion. Only 17 sporadic case-reports have been published to describe this disease. We report a retrospective cohort study of nine patients undergone surgical dissections in our hospital with labial minora fusion of reproductive age. General information, a medical history, gynecological examinations, preoperative
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The Influence Mechanism of Abnormal Immunophilin FKBP52 on the Expression Levels of PR-A and PR-B in Endometriosis Based on Endometrial Stromal Cell Model in Vitro Organogenesis (IF 2.3) Pub Date : 2021-01-19 Liling Liu, Junping Cheng, Fu Wei, Lihong Pang, Zhifu Zhi, Wenmei Yang, Weihong Tan
ABSTRACT As a chaperone protein of progesterone receptor (PR), FK-506 Binding Protein 52 (FKBP52) can enhance the activity of PR, but the mechanism of FKBP52 affecting PR expression levels is difficult to clarify. Here, we report a novel in vitro model of ectopic endometrial stromal cells (ESCM) established through the primary culture method of endometrial stromal cells, which is used to study the
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The Influence Mechanism of Abnormal Immunophilin FKBP52 on the Expression Levels of PR-A and PR-B in Endometriosis Based on Endometrial Stromal Cell Model in Vitro Organogenesis (IF 2.3) Pub Date : 2021-01-19 Liling Liu, Junping Cheng, Fu Wei, Lihong Pang, Zhifu Zhi, Wenmei Yang, Weihong Tan
ABSTRACT As a chaperone protein of progesterone receptor (PR), FK-506 Binding Protein 52 (FKBP52) can enhance the activity of PR, but the mechanism of FKBP52 affecting PR expression levels is difficult to clarify. Here, we report a novel in vitro model of ectopic endometrial stromal cells (ESCM) established through the primary culture method of endometrial stromal cells, which is used to study the
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An Evaluation of Wound Healing Efficacy of a Film Dressing Made from Polymer-integrated Amnion Membrane Organogenesis (IF 2.3) Pub Date : 2020-11-08 Bo Wang, Wuwei Li, Justin Harrison
ABSTRACT A film dressing is an easy and common wound management, which is flexible to cover many types of superficial injuries. In a recent study, we developed a scaffold from poly (1,8-octanediolco-citrate) incorporated decellularized amnion membrane (DAM-POC). The DAM-POC scaffold was biocompatible and could enhance soft and hard tissue regeneration when applied to repair the cleft palate in rat
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Differentiation of Human Parthenogenetic Embryonic Stem Cells into Functional Hepatocyte-like Cells Organogenesis (IF 2.3) Pub Date : 2020-11-25 Rui Liang, Zhiqiang Wang, Xiangyang Kong, Xiaoxiao Xiao, Tianxing Chen, Hui Yang, Ying Li, Xingqi Zhao
ABSTRACT Stem cell and tissue engineering-based therapies for acute liver failure (ALF) have been limited by the lack of an optimal cell source. We aimed to determine the suitability of human parthenogenetic embryonic stem cells (hPESCs) for the development of strategies to treat ALF. We studied the ability of human parthenogenetic embryonic stem cells (hPESCs) with high whole-genome SNP homozygosity
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Airway reconstruction using decellularized aortic xenografts in a dog model. Organogenesis (IF 2.3) Pub Date : 2020-07-16 Shao-Fei Cheng,Song Wu,Qian-Ping Li,Hong-Yang Sang,Zheng-Yang Fan
Tracheal reconstruction after extensive resection remains a challenge in thoracic surgery. Aortic allograft has been proposed to be a potential tracheal substitute. However, clinically, its application is limited for the shortage of autologous aortic segment. Whether xenogeneic aortic biosheets can be used as tracheal substitutes remains unknown. In the present study, we investigated the possibility
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The Effects of Ischemia and Hyperoxygenation on Hair Growth and Cycle. Organogenesis (IF 2.3) Pub Date : 2020-07-30 Harunosuke Kato,Kahori Kinoshita,Natsumi Saito,Koji Kanayama,Masanori Mori,Natsumi Asahi,Ataru Sunaga,Katsutoshi Yoshizato,Satoshi Itami,Kotaro Yoshimura
Alopecia has several causes, but its relationship with ischemia/hypoxia has not yet been investigated in detail. In this study, we studied the changes of hair follicles induced by ischemia and potential effects of normobaric hyperoxygenation (NBO) on the hair cycle and growth. We found that skin ischemia reduced hair growth rate, hair shaft size, and its pigmentation in the anagen phase of mice, which
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Surgical Models to Explore Acellular Liver Scaffold Transplantation: Step-by-Step. Organogenesis (IF 2.3) Pub Date : 2020-08-15 Marlon L Dias,Cíntia M P Batista,Victor J K Secomandi,Alexandre C Silva,Victoria R S Monteiro,Lanuza A Faccioli,Regina C S Goldenberg
Acellular liver scaffolds (ALS) have arisen as potential candidates for transplantation. Until now, all reports involving ALS transplantation failed in surgical method descriptions and do not offer support to scientists to reproduce the procedures used in experimental microsurgery to make the results comparable to literature. To overcome the lack of detail information, we described surgical steps details
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Recent Advances in Biomaterials for the Treatment of Bone Defects Organogenesis (IF 2.3) Pub Date : 2020-08-16 Le-Yi Zhang, Qing Bi, Chen Zhao, Jin-Yang Chen, Mao-Hua Cai, Xiao-Yi Chen
Bone defects or fractures generally heal in the absence of major interventions due to the high regenerative capacity of bone tissue. However, in situations of severe/large bone defects, these orchestrated regeneration mechanisms are impaired. With advances in modern medicine, natural and synthetic bio-scaffolds from bioceramics and polymers that support bone growth have emerged and gained intense research
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Recent Advances in Biomaterials for the Treatment of Bone Defects. Organogenesis (IF 2.3) Pub Date : 2020-08-16 Le-Yi Zhang,Qing Bi,Chen Zhao,Jin-Yang Chen,Mao-Hua Cai,Xiao-Yi Chen
ABSTRACT Bone defects or fractures generally heal in the absence of major interventions due to the high regenerative capacity of bone tissue. However, in situations of severe/large bone defects, these orchestrated regeneration mechanisms are impaired. With advances in modern medicine, natural and synthetic bio-scaffolds from bioceramics and polymers that support bone growth have emerged and gained
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Surgical Models to Explore Acellular Liver Scaffold Transplantation: Step-by-Step Organogenesis (IF 2.3) Pub Date : 2020-08-15 Marlon L. Dias, Cíntia M. P. Batista, Victor J. K. Secomandi, Alexandre C. Silva, Victoria R. S. Monteiro, Lanuza A. Faccioli, Regina C. S. Goldenberg
Acellular liver scaffolds (ALS) have arisen as potential candidates for transplantation. Until now, all reports involving ALS transplantation failed in surgical method descriptions and do not offer support to scientists to reproduce the procedures used in experimental microsurgery to make the results comparable to literature. To overcome the lack of detail information, we described surgical steps details
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Development of Bioengineered Organ Using Biological Acellular Rat Liver Scaffold and Hepatocytes. Organogenesis (IF 2.3) Pub Date : 2020-05-03 Tanya Debnath,Chandra Shekar Mallarpu,Lakshmi Kiran Chelluri
The increasing demand for organs for transplantation necessitates the development of substitutes to meet the structural and physiological functions. Tissue decellularization and recellularization aids in retaining the three-dimensional integrity, biochemical composition, tissue ultra-structure, and mechanical behavior, which makes them functionally suitable for organ transplantation. Herein, we attempted
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Evaluation of Radiosterilized Glyercerolated Amniotic Membranes as a Substrate for Cultured Human Epithelial Cells. Organogenesis (IF 2.3) Pub Date : 2020-02-15 André O Paggiaro,Monica B Mathor,Walcy R Teodoro,Cesár Isaac,Vera L Capelozzi,Rolf Gemperli
Human amniotic membrane (HAM) is a biomaterial with biological properties beneficial to tissue repair, serving as a substrate for cell cultivation. Irradiation is used for tissue sterilization, but can damage the HAM structure. The objective of this paper was to construct a skin substitute, composed of human keratinocytes cultured on glycerolated HAMs, and to evaluate the influence radiation on subsequent
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Influence of Different Cell Types and Sources on Pre-Vascularisation in Fibrin and Agarose–Collagen Gels Organogenesis (IF 2.3) Pub Date : 2019-12-06 Caroline Kniebs, Franziska Kreimendahl, Marius Köpf, Horst Fischer, Stefan Jockenhoevel, Anja Lena Thiebes
ABSTRACT Vascularisation is essential for the development of tailored, tissue-engineered organs and tissues due to diffusion limits of nutrients and the lack of the necessary connection to the cardiovascular system. To pre-vascularize, endothelial cells and supporting cells can be embedded in the scaffold to foster an adequate nutrient and oxygen supply after transplantation. This technique is applied
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Development of Small-diameter Polyester Vascular Grafts Coated with Silk Fibroin Sponge. Organogenesis (IF 2.3) Pub Date : 2019-11-02 Takashi Tanaka,Ryo Tanaka,Yoko Ogawa,Yoshihide Takagi,Tetsuo Asakura
In recent years, the demand for functional small-diameter (< 6 mm) artificial vascular grafts has greatly increased due to an increase in the number of patients with vascular heart disease. However, currently, there are no available commercial small-diameter grafts. The objective of this research was to develop a porous silk fibroin (SF)-coated poly(ethylene terephthalate) (PET) graft with a diameter
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Extracellular Matrix Remodeling During Palate Development. Organogenesis (IF 2.3) Pub Date : 2020-03-31 Xia Wang,Chunman Li,Zeyao Zhu,Li Yuan,Wood Yee Chan,Ou Sha
ABSTRACT The morphogenesis of the mammalian secondary plate is a series of highly dynamic developmental process, including the palate shelves vertical outgrowth, elevation to the horizontal plane and complete fusion in the midline. Extracellular matrix (ECM) proteins not only form the basic infrastructure for palatal mesenchymal cells to adhere via integrins but also interact with cells to regulate
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Characterization and in vivo study of decellularized aortic scaffolds using closed sonication system. Organogenesis (IF 2.3) Pub Date : 2019-09-07 Aqilah Hazwani,Munirah Sha'Ban,Azran Azhim
Extracellular matrix (ECM) based bioscaffolds prepared by decellularization has increasingly emerged in tissue engineering application because it has structural, biochemical, and biomechanical cues that have dramatic effects upon cell behaviors. Therefore, we developed a closed sonication decellularization system to prepare ideal bioscaffolds with minimal adverse effects on the ECM. The decellularization
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Advances of Wnt signalling pathway in dental development and potential clinical application. Organogenesis (IF 2.3) Pub Date : 2019-09-04 Xi Lu,Jun Yang,Shouliang Zhao,Shangfeng Liu
Wnt signalling pathway is widely studied in many processes of biological development, like embryogenesis, tissue homeostasis and wound repair. It is universally known that Wnt signalling pathway plays an important role in tooth development. Here, we summarized the function of Wnt signalling pathway during tooth initiation, crown morphogenesis, root formation, and discussed the therapeutic potential
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Specification of Sprouty2 functions in osteogenesis in in vivo context. Organogenesis (IF 2.3) Pub Date : 2019-09-04 Barbora Vesela,Eva Svandova,Maria Hovorakova,Renata Peterkova,Adela Kratochvilova,Martina Pasovska,Alice Ramesova,Herve Lesot,Eva Matalova
Sprouty proteins are modulators of the MAPK/ERK pathway. Amongst these, Sprouty2 (SPRY2) has been investigated as a possible factor that takes part in the initial phases of osteogenesis. However, the in vivo context has not yet been investigated and the underlying mechanisms taking place in vitro remain unknown. Therefore, in this study, the impact of Spry2 deficiency was examined in the developing
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In vivo construction of lymphoid node by implantation of adipose-derived stromal cells with hydroxypropyl methyl cellulose hydrogel in BALB/c nude mice. Organogenesis (IF 2.3) Pub Date : 2019-08-26 Jing Zhang,Yuqiao Xu,Tao Liu,Jie Min,Yu Ma,Yongli Song,Jianrong Lu,Wenjuan Mi,Yingmei Wang,Hang Li,Wangzhou Li,Daqing Zhao
Adipose-derived stromal cells have multilineage potential to differentiate into several specialized tissue types. Herein, we investigated whether ADSCs could differentiate into lymphoid node in vivo. Human ADSCs from routine liposuction were cultured in differentiation medium and were supplemented with transforming growth factor β1 (TGF)-β1 and basic fibroblast growth factor (bFGF). The induced hADSCs
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Expression and role of HIF-1α and HIF-2α in tissue regeneration: a study of hypoxia in house gecko tail regeneration. Organogenesis (IF 2.3) Pub Date : 2019-08-13 Titta Novianti,Vetnizah Juniantito,Ahmad Aulia Jusuf,Evy Ayu Arida,Sri Widia A Jusman,Mohamad Sadikin
The house gecko (Hemidactylus platyurus) has evolved the ability to autotomize its tail when threatened. The lost part is then regrown via epimorphic regeneration in a process that requires high energy and oxygen levels. Oxygen demand is therefore likely to outstrip supply and this can result in relative hypoxia in the tissues of the regenerating tail. The hypoxic state is stabilized by the Hypoxia
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Regeneration of caudal fin in Poecilia latipinna: Insights into the progressive tissue morphogenesis. Organogenesis (IF 2.3) Pub Date : 2019-07-22 Sonam Patel,Isha Ranadive,Isha Desai,Suresh Balakrishnan
Studies using fish fin as a model to understand the nuance of epimorphosis are gaining interest of lately. This study illustrates for the first time the daily changes in the tissue architecture of regenerating tail fin of Poecilia latipinna. Wound epithelium is formed within 24 hpa that eventually gets stratified into apical epithelial cap by 48 hpa. In the subsequent day, proliferating cells accumulate
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Anatomical structure, and expression of CCL4 and CCL13-like during the development of maxillary barbel in Paramisgurnus dabryanus. Organogenesis (IF 2.3) Pub Date : 2019-07-06 Kianann Tan,Ruijing Geng,Zhiqiang Wang,Han Liu,Weimin Wang
Paramisgurnus dabryanus is one of the most economically important fishes in China. Barbels are an essential sensory organ for the food-seeking ability of teleost fish. However, the anatomical structure of the maxillary barbels of P. dabryanus and the molecular basis of their development are unknown. We investigated the anatomical structure of the barbel, and gene expression patterns of two chemokine
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Ectopic localization of autophagosome in fatty liver is a key factor for liver regeneration. Organogenesis (IF 2.3) Pub Date : 2019-07-06 Yoshihiro Matsumoto,Tomoharu Yoshizumi,Takeo Toshima,Kazuki Takeishi,Takasuke Fukuhara,Shinji Itoh,Toru Ikegami,Yuji Soejima,Masaki Mori
Autophagy has a critical role in liver regeneration. However, no studies have demonstrated autophagic flux in the regenerating fatty liver. The aim of this study was to clarify the dynamics of autophagy in the regeneration of the fatty liver. Following 70% partial hepatectomy (PH) in db/db fatty mice, which is a non-alcoholic fatty liver disease (NAFLD) model, we investigated the survival rate and
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ATP6V1H facilitates osteogenic differentiation in MC3T3-E1 cells via Akt/GSK3β signaling pathway. Organogenesis (IF 2.3) Pub Date : 2019-07-04 Fusong Jiang,Haojie Shan,Chenhao Pan,Zubin Zhou,Keze Cui,Yuanliang Chen,Haibo Zhong,Zhibin Lin,Nan Wang,Liang Yan,Xiaowei Yu
Type 2 diabetes mellitus (T2DM) accounts for approximately 90% of all diabetic patients, and osteoporosis is one of the complications during T2DM process. ATP6V1H (V-type proton ATPase subunit H) displays crucial roles in inhibiting bone loss, but its role in osteogenic differentiation remains unknown. Therefore in this study, we aimed to explore the biological role of ATP6V1H in osteogenic differentiation
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Mesenchymal Wnt/β-catenin signaling induces Wnt and BMP antagonists in dental epithelium. Organogenesis (IF 2.3) Pub Date : 2019-06-26 Xiaoyan Chen,Jing Liu,Nan Li,Yu Wang,Nan Zhou,Lei Zhu,Yiding Shi,Yingzhang Wu,Jing Xiao,Chao Liu
Previous studies indicated that the elevated mesenchymal Wnt/β-catenin signaling deprived dental mesenchyme of odontogenic fate. By utilizing ex vivo or pharmacological approaches, Wnt/β-catenin signaling in the developing dental mesenchyme was suggested to suppress the odontogenic fate by disrupting the balance between Axin2 and Runx2. In our study, the Osr2-creKI; Ctnnb1ex3f mouse was used to explore
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Persistent Wnt/β-catenin signaling in mouse epithelium induces the ectopic Dspp expression in cheek mesenchyme. Organogenesis (IF 2.3) Pub Date : 2018-12-20 Nan Zhou,Nan Li,Jing Liu,Yu Wang,Jun Gao,Yingzhang Wu,Xiaoyan Chen,Chao Liu,Jing Xiao
Tooth development is accomplished by a series of epithelial-mesenchyme interactions. Epithelial Wnt/β-catenin signaling is sufficient to initiate tooth development by activating Shh, Bmps, Fgfs and Wnts in dental epithelium, which in turn, triggered the expression of odontogenic genes in the underlying mesenchyme. Although constitutive activation of Wnt/β-catenin signaling in oral ectoderm resulted
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Normothermic ex vivo liver perfusion: platform for liver graft assessment and therapeutic modification. Organogenesis (IF 2.3) Pub Date : 2018-10-05 Corey Eymard,James Markmann
Liver transplantation as a treatment for end stage liver failure remains limited in the United States by the number and quality of donor allografts. Static cold storage, the current standard of care for organ storage prior to transplantation, offers no method for assessment or therapeutic modification. Cold ischemia and its attendant hypoxia deplete cellular adenosine triphosphate (ATP) stores, promote
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Rebuilding a better home for transplanted islets. Organogenesis (IF 2.3) Pub Date : 2018-09-25 Daniel M Tremmel,Jon S Odorico
Diabetes can be treated with β cell replacement therapy, where a patient is transplanted with cadaveric human islets to restore glycemic control. Despite this being an effective treatment, the process of isolating islets from the pancreas requires collagenase digestion which disrupts the islet extracellular matrix (ECM) and activates anoikis-mediated apoptosis. To improve islet survival in culture
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Xenotransplantation. Organogenesis (IF 2.3) Pub Date : 2018-09-25 Srijan Tandukar,Sundaram Hariharan
The transplantation of living cells, tissues or organs from one species to another is termed xenotransplantation. The history of xenotransplantation is as old as allogeneic transplantation itself. Early attempts were made at a time when the immunologic basis of organ rejection were poorly understood. The advent of potent immunosuppressive medications along with the parallel advances in the field of
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Utility of extracellular matrix powders in tissue engineering. Organogenesis (IF 2.3) Pub Date : 2018-09-05 Lauren Edgar,Afnan Altamimi,Marta García Sánchez,Riccardo Tamburrinia,Amish Asthana,Carlo Gazia,Giuseppe Orlando
Extracellular matrix (ECM) materials have had remarkable success as scaffolds in tissue engineering (TE) and as therapies for tissue injury whereby the ECM microenvironment promotes constructive remodeling and tissue regeneration. ECM powder and solubilized derivatives thereof have novel applications in TE and RM afforded by the capacity of these constructs to be dynamically modulated. The powder form
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Bioinspired liver scaffold design criteria. Organogenesis (IF 2.3) Pub Date : 2018-08-29 Giorgio Mattei,Chiara Magliaro,Andrea Pirone,Arti Ahluwalia
Maintaining hepatic functional characteristics in-vitro is considered one of the main challenges in engineering liver tissue. As hepatocytes cultured ex-vivo are deprived of their native extracellular matrix (ECM) milieu, developing scaffolds that mimic the biomechanical and physicochemical properties of the native ECM is thought to be a promising approach for successful tissue engineering and regenerative