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An Evaluation of Wound Healing Efficacy of a Film Dressing Made from Polymer-integrated Amnion Membrane Organogenesis (IF 2.321) Pub Date : 2020-11-08 Bo Wang; Wuwei Li; Justin Harrison
ABSTRACT A film dressing is an easy and common wound management, which is flexible to cover many types of superficial injuries. In a recent study, we developed a scaffold from poly (1,8-octanediolco-citrate) incorporated decellularized amnion membrane (DAM-POC). The DAM-POC scaffold was biocompatible and could enhance soft and hard tissue regeneration when applied to repair the cleft palate in rat
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Differentiation of Human Parthenogenetic Embryonic Stem Cells into Functional Hepatocyte-like Cells Organogenesis (IF 2.321) Pub Date : 2020-11-25 Rui Liang; Zhiqiang Wang; Xiangyang Kong; Xiaoxiao Xiao; Tianxing Chen; Hui Yang; Ying Li; Xingqi Zhao
ABSTRACT Stem cell and tissue engineering-based therapies for acute liver failure (ALF) have been limited by the lack of an optimal cell source. We aimed to determine the suitability of human parthenogenetic embryonic stem cells (hPESCs) for the development of strategies to treat ALF. We studied the ability of human parthenogenetic embryonic stem cells (hPESCs) with high whole-genome SNP homozygosity
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Airway reconstruction using decellularized aortic xenografts in a dog model. Organogenesis (IF 2.321) Pub Date : 2020-07-16 Shao-Fei Cheng,Song Wu,Qian-Ping Li,Hong-Yang Sang,Zheng-Yang Fan
Tracheal reconstruction after extensive resection remains a challenge in thoracic surgery. Aortic allograft has been proposed to be a potential tracheal substitute. However, clinically, its application is limited for the shortage of autologous aortic segment. Whether xenogeneic aortic biosheets can be used as tracheal substitutes remains unknown. In the present study, we investigated the possibility
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The Effects of Ischemia and Hyperoxygenation on Hair Growth and Cycle. Organogenesis (IF 2.321) Pub Date : 2020-07-30 Harunosuke Kato,Kahori Kinoshita,Natsumi Saito,Koji Kanayama,Masanori Mori,Natsumi Asahi,Ataru Sunaga,Katsutoshi Yoshizato,Satoshi Itami,Kotaro Yoshimura
Alopecia has several causes, but its relationship with ischemia/hypoxia has not yet been investigated in detail. In this study, we studied the changes of hair follicles induced by ischemia and potential effects of normobaric hyperoxygenation (NBO) on the hair cycle and growth. We found that skin ischemia reduced hair growth rate, hair shaft size, and its pigmentation in the anagen phase of mice, which
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Surgical Models to Explore Acellular Liver Scaffold Transplantation: Step-by-Step. Organogenesis (IF 2.321) Pub Date : 2020-08-15 Marlon L Dias,Cíntia M P Batista,Victor J K Secomandi,Alexandre C Silva,Victoria R S Monteiro,Lanuza A Faccioli,Regina C S Goldenberg
Acellular liver scaffolds (ALS) have arisen as potential candidates for transplantation. Until now, all reports involving ALS transplantation failed in surgical method descriptions and do not offer support to scientists to reproduce the procedures used in experimental microsurgery to make the results comparable to literature. To overcome the lack of detail information, we described surgical steps details
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Recent Advances in Biomaterials for the Treatment of Bone Defects Organogenesis (IF 2.321) Pub Date : 2020-08-16 Le-Yi Zhang; Qing Bi; Chen Zhao; Jin-Yang Chen; Mao-Hua Cai; Xiao-Yi Chen
Bone defects or fractures generally heal in the absence of major interventions due to the high regenerative capacity of bone tissue. However, in situations of severe/large bone defects, these orchestrated regeneration mechanisms are impaired. With advances in modern medicine, natural and synthetic bio-scaffolds from bioceramics and polymers that support bone growth have emerged and gained intense research
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Recent Advances in Biomaterials for the Treatment of Bone Defects. Organogenesis (IF 2.321) Pub Date : 2020-08-16 Le-Yi Zhang,Qing Bi,Chen Zhao,Jin-Yang Chen,Mao-Hua Cai,Xiao-Yi Chen
ABSTRACT Bone defects or fractures generally heal in the absence of major interventions due to the high regenerative capacity of bone tissue. However, in situations of severe/large bone defects, these orchestrated regeneration mechanisms are impaired. With advances in modern medicine, natural and synthetic bio-scaffolds from bioceramics and polymers that support bone growth have emerged and gained
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Surgical Models to Explore Acellular Liver Scaffold Transplantation: Step-by-Step Organogenesis (IF 2.321) Pub Date : 2020-08-15 Marlon L. Dias; Cíntia M. P. Batista; Victor J. K. Secomandi; Alexandre C. Silva; Victoria R. S. Monteiro; Lanuza A. Faccioli; Regina C. S. Goldenberg
Acellular liver scaffolds (ALS) have arisen as potential candidates for transplantation. Until now, all reports involving ALS transplantation failed in surgical method descriptions and do not offer support to scientists to reproduce the procedures used in experimental microsurgery to make the results comparable to literature. To overcome the lack of detail information, we described surgical steps details
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Development of Bioengineered Organ Using Biological Acellular Rat Liver Scaffold and Hepatocytes. Organogenesis (IF 2.321) Pub Date : 2020-05-03 Tanya Debnath,Chandra Shekar Mallarpu,Lakshmi Kiran Chelluri
The increasing demand for organs for transplantation necessitates the development of substitutes to meet the structural and physiological functions. Tissue decellularization and recellularization aids in retaining the three-dimensional integrity, biochemical composition, tissue ultra-structure, and mechanical behavior, which makes them functionally suitable for organ transplantation. Herein, we attempted
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Evaluation of Radiosterilized Glyercerolated Amniotic Membranes as a Substrate for Cultured Human Epithelial Cells. Organogenesis (IF 2.321) Pub Date : 2020-02-15 André O Paggiaro,Monica B Mathor,Walcy R Teodoro,Cesár Isaac,Vera L Capelozzi,Rolf Gemperli
Human amniotic membrane (HAM) is a biomaterial with biological properties beneficial to tissue repair, serving as a substrate for cell cultivation. Irradiation is used for tissue sterilization, but can damage the HAM structure. The objective of this paper was to construct a skin substitute, composed of human keratinocytes cultured on glycerolated HAMs, and to evaluate the influence radiation on subsequent
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Influence of Different Cell Types and Sources on Pre-Vascularisation in Fibrin and Agarose–Collagen Gels Organogenesis (IF 2.321) Pub Date : 2019-12-06 Caroline Kniebs; Franziska Kreimendahl; Marius Köpf; Horst Fischer; Stefan Jockenhoevel; Anja Lena Thiebes
ABSTRACT Vascularisation is essential for the development of tailored, tissue-engineered organs and tissues due to diffusion limits of nutrients and the lack of the necessary connection to the cardiovascular system. To pre-vascularize, endothelial cells and supporting cells can be embedded in the scaffold to foster an adequate nutrient and oxygen supply after transplantation. This technique is applied
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Development of Small-diameter Polyester Vascular Grafts Coated with Silk Fibroin Sponge. Organogenesis (IF 2.321) Pub Date : 2019-11-02 Takashi Tanaka,Ryo Tanaka,Yoko Ogawa,Yoshihide Takagi,Tetsuo Asakura
In recent years, the demand for functional small-diameter (< 6 mm) artificial vascular grafts has greatly increased due to an increase in the number of patients with vascular heart disease. However, currently, there are no available commercial small-diameter grafts. The objective of this research was to develop a porous silk fibroin (SF)-coated poly(ethylene terephthalate) (PET) graft with a diameter
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Extracellular Matrix Remodeling During Palate Development. Organogenesis (IF 2.321) Pub Date : 2020-03-31 Xia Wang,Chunman Li,Zeyao Zhu,Li Yuan,Wood Yee Chan,Ou Sha
ABSTRACT The morphogenesis of the mammalian secondary plate is a series of highly dynamic developmental process, including the palate shelves vertical outgrowth, elevation to the horizontal plane and complete fusion in the midline. Extracellular matrix (ECM) proteins not only form the basic infrastructure for palatal mesenchymal cells to adhere via integrins but also interact with cells to regulate
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Characterization and in vivo study of decellularized aortic scaffolds using closed sonication system. Organogenesis (IF 2.321) Pub Date : 2019-09-07 Aqilah Hazwani,Munirah Sha'Ban,Azran Azhim
Extracellular matrix (ECM) based bioscaffolds prepared by decellularization has increasingly emerged in tissue engineering application because it has structural, biochemical, and biomechanical cues that have dramatic effects upon cell behaviors. Therefore, we developed a closed sonication decellularization system to prepare ideal bioscaffolds with minimal adverse effects on the ECM. The decellularization
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Advances of Wnt signalling pathway in dental development and potential clinical application. Organogenesis (IF 2.321) Pub Date : 2019-09-04 Xi Lu,Jun Yang,Shouliang Zhao,Shangfeng Liu
Wnt signalling pathway is widely studied in many processes of biological development, like embryogenesis, tissue homeostasis and wound repair. It is universally known that Wnt signalling pathway plays an important role in tooth development. Here, we summarized the function of Wnt signalling pathway during tooth initiation, crown morphogenesis, root formation, and discussed the therapeutic potential
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Specification of Sprouty2 functions in osteogenesis in in vivo context. Organogenesis (IF 2.321) Pub Date : 2019-09-04 Barbora Vesela,Eva Svandova,Maria Hovorakova,Renata Peterkova,Adela Kratochvilova,Martina Pasovska,Alice Ramesova,Herve Lesot,Eva Matalova
Sprouty proteins are modulators of the MAPK/ERK pathway. Amongst these, Sprouty2 (SPRY2) has been investigated as a possible factor that takes part in the initial phases of osteogenesis. However, the in vivo context has not yet been investigated and the underlying mechanisms taking place in vitro remain unknown. Therefore, in this study, the impact of Spry2 deficiency was examined in the developing
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In vivo construction of lymphoid node by implantation of adipose-derived stromal cells with hydroxypropyl methyl cellulose hydrogel in BALB/c nude mice. Organogenesis (IF 2.321) Pub Date : 2019-08-26 Jing Zhang,Yuqiao Xu,Tao Liu,Jie Min,Yu Ma,Yongli Song,Jianrong Lu,Wenjuan Mi,Yingmei Wang,Hang Li,Wangzhou Li,Daqing Zhao
Adipose-derived stromal cells have multilineage potential to differentiate into several specialized tissue types. Herein, we investigated whether ADSCs could differentiate into lymphoid node in vivo. Human ADSCs from routine liposuction were cultured in differentiation medium and were supplemented with transforming growth factor β1 (TGF)-β1 and basic fibroblast growth factor (bFGF). The induced hADSCs
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Expression and role of HIF-1α and HIF-2α in tissue regeneration: a study of hypoxia in house gecko tail regeneration. Organogenesis (IF 2.321) Pub Date : 2019-08-13 Titta Novianti,Vetnizah Juniantito,Ahmad Aulia Jusuf,Evy Ayu Arida,Sri Widia A Jusman,Mohamad Sadikin
The house gecko (Hemidactylus platyurus) has evolved the ability to autotomize its tail when threatened. The lost part is then regrown via epimorphic regeneration in a process that requires high energy and oxygen levels. Oxygen demand is therefore likely to outstrip supply and this can result in relative hypoxia in the tissues of the regenerating tail. The hypoxic state is stabilized by the Hypoxia
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Regeneration of caudal fin in Poecilia latipinna: Insights into the progressive tissue morphogenesis. Organogenesis (IF 2.321) Pub Date : 2019-07-22 Sonam Patel,Isha Ranadive,Isha Desai,Suresh Balakrishnan
Studies using fish fin as a model to understand the nuance of epimorphosis are gaining interest of lately. This study illustrates for the first time the daily changes in the tissue architecture of regenerating tail fin of Poecilia latipinna. Wound epithelium is formed within 24 hpa that eventually gets stratified into apical epithelial cap by 48 hpa. In the subsequent day, proliferating cells accumulate
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Anatomical structure, and expression of CCL4 and CCL13-like during the development of maxillary barbel in Paramisgurnus dabryanus. Organogenesis (IF 2.321) Pub Date : 2019-07-06 Kianann Tan,Ruijing Geng,Zhiqiang Wang,Han Liu,Weimin Wang
Paramisgurnus dabryanus is one of the most economically important fishes in China. Barbels are an essential sensory organ for the food-seeking ability of teleost fish. However, the anatomical structure of the maxillary barbels of P. dabryanus and the molecular basis of their development are unknown. We investigated the anatomical structure of the barbel, and gene expression patterns of two chemokine
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Ectopic localization of autophagosome in fatty liver is a key factor for liver regeneration. Organogenesis (IF 2.321) Pub Date : 2019-07-06 Yoshihiro Matsumoto,Tomoharu Yoshizumi,Takeo Toshima,Kazuki Takeishi,Takasuke Fukuhara,Shinji Itoh,Toru Ikegami,Yuji Soejima,Masaki Mori
Autophagy has a critical role in liver regeneration. However, no studies have demonstrated autophagic flux in the regenerating fatty liver. The aim of this study was to clarify the dynamics of autophagy in the regeneration of the fatty liver. Following 70% partial hepatectomy (PH) in db/db fatty mice, which is a non-alcoholic fatty liver disease (NAFLD) model, we investigated the survival rate and
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ATP6V1H facilitates osteogenic differentiation in MC3T3-E1 cells via Akt/GSK3β signaling pathway. Organogenesis (IF 2.321) Pub Date : 2019-07-04 Fusong Jiang,Haojie Shan,Chenhao Pan,Zubin Zhou,Keze Cui,Yuanliang Chen,Haibo Zhong,Zhibin Lin,Nan Wang,Liang Yan,Xiaowei Yu
Type 2 diabetes mellitus (T2DM) accounts for approximately 90% of all diabetic patients, and osteoporosis is one of the complications during T2DM process. ATP6V1H (V-type proton ATPase subunit H) displays crucial roles in inhibiting bone loss, but its role in osteogenic differentiation remains unknown. Therefore in this study, we aimed to explore the biological role of ATP6V1H in osteogenic differentiation
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Mesenchymal Wnt/β-catenin signaling induces Wnt and BMP antagonists in dental epithelium. Organogenesis (IF 2.321) Pub Date : 2019-06-26 Xiaoyan Chen,Jing Liu,Nan Li,Yu Wang,Nan Zhou,Lei Zhu,Yiding Shi,Yingzhang Wu,Jing Xiao,Chao Liu
Previous studies indicated that the elevated mesenchymal Wnt/β-catenin signaling deprived dental mesenchyme of odontogenic fate. By utilizing ex vivo or pharmacological approaches, Wnt/β-catenin signaling in the developing dental mesenchyme was suggested to suppress the odontogenic fate by disrupting the balance between Axin2 and Runx2. In our study, the Osr2-creKI; Ctnnb1ex3f mouse was used to explore
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Persistent Wnt/β-catenin signaling in mouse epithelium induces the ectopic Dspp expression in cheek mesenchyme. Organogenesis (IF 2.321) Pub Date : 2018-12-20 Nan Zhou,Nan Li,Jing Liu,Yu Wang,Jun Gao,Yingzhang Wu,Xiaoyan Chen,Chao Liu,Jing Xiao
Tooth development is accomplished by a series of epithelial-mesenchyme interactions. Epithelial Wnt/β-catenin signaling is sufficient to initiate tooth development by activating Shh, Bmps, Fgfs and Wnts in dental epithelium, which in turn, triggered the expression of odontogenic genes in the underlying mesenchyme. Although constitutive activation of Wnt/β-catenin signaling in oral ectoderm resulted
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Normothermic ex vivo liver perfusion: platform for liver graft assessment and therapeutic modification. Organogenesis (IF 2.321) Pub Date : 2018-10-05 Corey Eymard,James Markmann
Liver transplantation as a treatment for end stage liver failure remains limited in the United States by the number and quality of donor allografts. Static cold storage, the current standard of care for organ storage prior to transplantation, offers no method for assessment or therapeutic modification. Cold ischemia and its attendant hypoxia deplete cellular adenosine triphosphate (ATP) stores, promote
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Rebuilding a better home for transplanted islets. Organogenesis (IF 2.321) Pub Date : 2018-09-25 Daniel M Tremmel,Jon S Odorico
Diabetes can be treated with β cell replacement therapy, where a patient is transplanted with cadaveric human islets to restore glycemic control. Despite this being an effective treatment, the process of isolating islets from the pancreas requires collagenase digestion which disrupts the islet extracellular matrix (ECM) and activates anoikis-mediated apoptosis. To improve islet survival in culture
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Xenotransplantation. Organogenesis (IF 2.321) Pub Date : 2018-09-25 Srijan Tandukar,Sundaram Hariharan
The transplantation of living cells, tissues or organs from one species to another is termed xenotransplantation. The history of xenotransplantation is as old as allogeneic transplantation itself. Early attempts were made at a time when the immunologic basis of organ rejection were poorly understood. The advent of potent immunosuppressive medications along with the parallel advances in the field of
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Utility of extracellular matrix powders in tissue engineering. Organogenesis (IF 2.321) Pub Date : 2018-09-05 Lauren Edgar,Afnan Altamimi,Marta García Sánchez,Riccardo Tamburrinia,Amish Asthana,Carlo Gazia,Giuseppe Orlando
Extracellular matrix (ECM) materials have had remarkable success as scaffolds in tissue engineering (TE) and as therapies for tissue injury whereby the ECM microenvironment promotes constructive remodeling and tissue regeneration. ECM powder and solubilized derivatives thereof have novel applications in TE and RM afforded by the capacity of these constructs to be dynamically modulated. The powder form
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Bioinspired liver scaffold design criteria. Organogenesis (IF 2.321) Pub Date : 2018-08-29 Giorgio Mattei,Chiara Magliaro,Andrea Pirone,Arti Ahluwalia
Maintaining hepatic functional characteristics in-vitro is considered one of the main challenges in engineering liver tissue. As hepatocytes cultured ex-vivo are deprived of their native extracellular matrix (ECM) milieu, developing scaffolds that mimic the biomechanical and physicochemical properties of the native ECM is thought to be a promising approach for successful tissue engineering and regenerative
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Application of drug delivery systems for the controlled delivery of growth factors to treat nervous system injury. Organogenesis (IF 2.321) Pub Date : 2018-08-27 Fukai Ma,Fan Wang,Ronggang Li,Jianhong Zhu
Nervous system injury represent the most common injury and was unique clinical challenge. Using of growth factors (GFs) for the treatment of nervous system injury showed effectiveness in halting its process. However, simple application of GFs could not achieve high efficacy because of its rapid diffusion into body fluids and lost from the lesion site. The drug delivery systems (DDSs) construction used
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Taurine enhances mouse cochlear neural stem cells proliferation and differentiation to sprial gangli through activating sonic hedgehog signaling pathway. Organogenesis (IF 2.321) Pub Date : 2018-08-13 Xinghua Huang,Weijing Wu,Peng Hu,Qin Wang
To investigate the molecular mechanism underlying taurine-stimulated proliferation and differentiation of cochlear neural stem cells (NSCs) and potential involvement of Sonic Hedgehog (Shh) pathway. The NSCs were characterized with immunofluorescence stained with nestin antibody. Cell viability was determined by MTT assay. The relative proliferation was measured by BrdU incorporation assay. The morphologic
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Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution. Organogenesis (IF 2.321) Pub Date : 2018-08-06 Gesine Pless-Petig,Sven Knoop,Ursula Rauen
Cryopreservation is the only long-term storage option for the storage of vessels and vascular constructs. However, endothelial barrier function is almost completely lost after cryopreservation in most established cryopreservation solutions. We here aimed to improve endothelial function after cryopreservation using the 2D-model of porcine aortic endothelial cell monolayers. The monolayers were cryopreserved
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Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/β-catenin and MAPK pathway. Organogenesis (IF 2.321) Pub Date : 2018-06-18 Hongpeng Yang,Yue Guo,Dawei Wang,Xiaofei Yang,Chengzhi Ha
Mesenchymal stem cells (MSCs) have the ability to differentiate into osteoblasts and chondrocytes. In vitro osteogenic differentiation is critical but the molecular mechanism has yet to be further clarified. The role of TGF-β activated kinase 1 (TAK1) in MSCs osteogenesis differentiation has not been reported. By adding si-TAK1 and rhTAK1, the osteogenic differentiation of MSCs was measured. Expression
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Celastrol enhances Atoh1 expression in inner ear stem cells and promotes their differentiation into functional auditory neuronal-like cells. Organogenesis (IF 2.321) Pub Date : 2018-06-14 Zhao Han,Yu-Yan Gu,Ning Cong,Rui Ma,Fang-Lu Chi
We aimed to investigate the beneficial effect of Celastrol on inner ear stem cells and potential therapeutic value for hearing loss. The inner ear stem cells were isolated and characterized from utricular sensory epithelium of adult mice. The stemness was evaluated by sphere formation assay. The relative expressions of Atoh1, MAP-2 and Myosin VI were measured by RT-PCR and immunoblotting. The up-regulation
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Sodium hydroxide based non-detergent decellularizing solution for rat lung. Organogenesis (IF 2.321) Pub Date : 2018-06-11 Hideyori Sengyoku,Tomoshi Tsuchiya,Tomohiro Obata,Ryoichiro Doi,Yasumasa Hashimoto,Mitsutoshi Ishii,Hiromi Sakai,Naoto Matsuo,Daisuke Taniguchi,Takashi Suematsu,Murray Lawn,Keitaro Matsumoto,Takuro Miyazaki,Takeshi Nagayasu
Lung transplantation is the last option for the treatment of end stage chronic lung disorders. Because the shortage of donor lung organs represents the main hurdle, lung regeneration has been considered to overcome this hurdle. Recellularization of decellularized organ scaffold is a promising option for organ regeneration. Although detergents are ordinarily used for decellularization, other approaches
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A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection. Organogenesis (IF 2.321) Pub Date : 2018-06-08 Fan Liu,Pawan Kc,Liwei Ni,Ge Zhang,Jiang Zhe
We report a high-sensitivity cell secretome detection method using competitive immuno-aggregation and a micro-Coulter counter. A target cell secretome protein competes with anti-biotin-coated microparticles (MPs) to bind with a biotinylated antibody (Ab), causing decreased aggregation of the functionalized MPs and formation of a mixture of MPs and aggregates. In comparison, without the target cell
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Effects of porcine acellular dermal matrix treatment on wound healing and scar formation: Role of Jag1 expression in epidermal stem cells. Organogenesis (IF 2.321) Pub Date : 2018-04-27 Xiao-Dong Chen,Shu-Bin Ruan,Ze-Peng Lin,Ziheng Zhou,Feng-Gang Zhang,Rong-Hua Yang,Ju-Lin Xie
Skin wound healing involves Notch/Jagged1 signaling. However, little is known how Jag1 expression level in epidermal stem cells (ESCs) contributes to wound healing and scar formation. We applied multiple cellular and molecular techniques to examine how Jag1 expression in ESCs modulates ESCs differentiation to myofibroblasts (MFB) in vitro, interpret how Jag1 expression in ESCs is involved in wound
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Suppression of LRRC19 promotes cutaneous wound healing in pressure ulcers in mice. Organogenesis (IF 2.321) Pub Date : 2018-02-20 Jie Sun,Zhijing Wang,Xirui Wang
The ischemia-reperfusion (I/R) induced skin lesion has been identified as primary cause of pressure ulcer. Better understanding of the mechanism is required for new therapy development. Leucine rich repeat containing protein 19 (LRRC19) is a recently discovered transmembrane protein containing leucine-rich repeats and plays a role in immune response. To investigate the role of LRRC19 in pressure ulcers
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Embryonic skin development and repair. Organogenesis (IF 2.321) Pub Date : 2018-02-15 Michael S Hu,Mimi R Borrelli,Wan Xing Hong,Samir Malhotra,Alexander T M Cheung,Ryan C Ransom,Robert C Rennert,Shane D Morrison,H Peter Lorenz,Michael T Longaker
Fetal cutaneous wounds have the unique ability to completely regenerate wounded skin and heal without scarring. However, adult cutaneous wounds heal via a fibroproliferative response which results in the formation of a scar. Understanding the mechanism(s) of scarless wound healing leads to enormous clinical potential in facilitating an environment conducive to scarless healing in adult cutaneous wounds
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Prolongation of liver-specific function for primary hepatocytes maintenance in 3D printed architectures. Organogenesis (IF 2.321) Pub Date : 2018-02-01 Yohan Kim,Kyojin Kang,Sangtae Yoon,Ji Sook Kim,Su A Park,Wan Doo Kim,Seung Bum Lee,Ki-Young Ryu,Jaemin Jeong,Dongho Choi
Isolated primary hepatocytes from the liver are very similar to in vivo native liver hepatocytes, but they have the disadvantage of a limited lifespan in 2D culture. Although a sandwich culture and 3D organoids with mesenchymal stem cells (MSCs) as an attractive assistant cell source to extend lifespan can be used, it cannot fully reproduce the in vivo architecture. Moreover, long-term 3D culture leads
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Corrigendum. Organogenesis (IF 2.321) Pub Date : 2018-01-30
(2018). Corrigendum. Organogenesis: Vol. 14, No. 1, pp. 65-65.
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Erratum. Organogenesis (IF 2.321) Pub Date : 2017-12-06
(2018). Erratum. Organogenesis: Vol. 14, No. 1, pp. 64-64.
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EphB receptors, mainly EphB3, contribute to the proper development of cortical thymic epithelial cells. Organogenesis (IF 2.321) Pub Date : 2017-11-15 Sara Montero-Herradón,Javier García-Ceca,Agustín G Zapata
EphB and their ligands ephrin-B are an important family of protein tyrosine kinase receptors involved in thymocyte-thymic epithelial cell interactions known to be key for the maturation of both thymic cell components. In the present study, we have analyzed the maturation of cortical thymic epithelium in EphB-deficient thymuses evaluating the relative relevance of EphB2 and EphB3 in the process. Results
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Factors and molecules that could impact cell differentiation in the embryo generated by nuclear transfer. Organogenesis (IF 2.321) Pub Date : 2017-11-02 Renata Simões,Arnaldo Rodrigues Santos
Somatic cell nuclear transfer is a technique to create an embryo using an enucleated oocyte and a donor nucleus. Nucleus of somatic cells must be reprogrammed in order to participate in normal development within an enucleated egg. Reprogramming refers to the erasing and remodeling of cellular epigenetic marks to a lower differentiation state. Somatic nuclei must be reprogrammed by factors in the oocyte
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Corrigendum. Organogenesis (IF 2.321) Pub Date : 2017-10-24
(2017). Corrigendum. Organogenesis: Vol. 13, No. 3, pp. 114-114.
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Upper limb hemimelia in a twin pregnancy which was obtained by an ICSI and PGD in a woman with mosaic Turner's syndrome and the prognosis. Organogenesis (IF 2.321) Pub Date : 2017-10-13 Ahter Tanay Tayyar,Ahmet Tayyar,Ahmet Eser,Çetin Kılıçcı,İlter Yenidede,Selçuk Selçuk
Turner's syndrome (TS) is depicted as a total or partial absence of X chromosome, and occurs in approximately 1/2200 of live born females. Generally, mosaic patients are diagnosed following karyotype analysis due to recurrent pregnancy loss, repeated in vitro fertilization (IVF) failure, and a history of malformed babies. The purpose of this case report is to show that even a selection of normal karyotype
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Regulation of proliferation in developing human tooth germs by MSX homeodomain proteins and cyclin-dependent kinase inhibitor p19INK4d. Organogenesis (IF 2.321) Pub Date : 2017-10-13 Darko Kero,Katarina Vukojevic,Petra Stazic,Danijela Sundov,Snjezana Mardesic Brakus,Mirna Saraga-Babic
Before the secretion of hard dental tissues, tooth germs undergo several distinctive stages of development (dental lamina, bud, cap and bell). Every stage is characterized by specific proliferation patterns, which is regulated by various morphogens, growth factors and homeodomain proteins. The role of MSX homeodomain proteins in odontogenesis is rather complex. Expression domains of genes encoding
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RARα and RARγ reciprocally control K5+ progenitor cell expansion in developing salivary glands. Organogenesis (IF 2.321) Pub Date : 2017-10-13 Kara A DeSantis,Adam R Stabell,Danielle C Spitzer,Kevin J O'Keefe,Deirdre A Nelson,Melinda Larsen
Understanding the mechanisms of controlled expansion and differentiation of basal progenitor cell populations during organogenesis is essential for developing targeted regenerative therapies. Since the cytokeratin 5-positive (K5+) basal epithelial cell population in the salivary gland is regulated by retinoic acid signaling, we interrogated how isoform-specific retinoic acid receptor (RAR) signaling
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The effect of a silk Fibroin/Polyurethane blend patch on rat Vessels. Organogenesis (IF 2.321) Pub Date : 2017-10-13 Kazumi Shimada,Akira Higuchi,Ryota Kubo,Tomoaki Murakami,Yasumoto Nakazawa,Ryou Tanaka
Patch grafts are widely used in various kind of vascular surgeries such as detect repair or dilation of vascular stenosis. Expanded polytetrafluoroethylene (ePTFE) patches are flexible and handle well, but have shown problems with calcification as they are non-bioabsorbable and therefore permanently remain in the body. It is important to develop an alternative biocompatible patch. Silk fibroin (SF)
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Nicotinic acid inhibits angiogenesis likely through cytoskeleton remodeling. Organogenesis (IF 2.321) Pub Date : 2017-10-11 Lemen Pan,Guanfeng Yu,Xiangjian Chen,Xiaoqiang Li
Angiogenesis is a physiological procedure during which the new blood vessels develop from the pre-existing vessels. Uncontrolled angiogenesis is related to various diseases including cancers. Clinical inhibition of undesired angiogenesis is still under investigation. We utilized nicotinic acid, a family member of the B-vitamin niacin (vitamin B3) that has been used in the prevention and treatment of
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The neuronal differentiation microenvironment is essential for spinal cord injury repair. Organogenesis (IF 2.321) Pub Date : 2017-08-24 Yannan Zhao,Zhifeng Xiao,Bing Chen,Jianwu Dai
Spinal cord injury (SCI) often leads to substantial disability due to loss of motor function and sensation below the lesion. Neural stem cells (NSCs) are a promising strategy for SCI repair. However, NSCs rarely differentiate into neurons; they mostly differentiate into astrocytes because of the adverse microenvironment present after SCI. We have shown that myelin-associated inhibitors (MAIs) inhibited
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The epidemiology of supernumerary teeth and the associated molecular mechanism. Organogenesis (IF 2.321) Pub Date : 2017-08-24 Xi Lu,Fang Yu,Junjun Liu,Wenping Cai,Yumei Zhao,Shouliang Zhao,Shangfeng Liu
Supernumerary teeth are common clinical dental anomalies. Although various studies have provided abundant information regarding genes and signaling pathways involved in tooth morphogenesis, which include Wnt, FGF, BMP, and Shh, the molecular mechanism of tooth formation, especially for supernumerary teeth, is still unclear. In the population, some cases of supernumerary teeth are sporadic, while others
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Enamel matrix derivative enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells on the titanium implant surface. Organogenesis (IF 2.321) Pub Date : 2017-08-24 Guang Li,Jing Hu,Hui Chen,Liang Chen,Na Zhang,Lisheng Zhao,Ning Wen,Yongjin Yang
Periodontal ligament stem cells (PDLSCs) have mesenchymal-stem-cells-like qualities, and are considered as one of the candidates of future clinical application in periodontal regeneration therapy. Enamel matrix derivative (EMD) is widely used in promoting periodontal regeneration. However, the effects of EMD on the proliferation and osteogenic differentiation of human PDLSCs grown on the Ti implant
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Endometriosis origin from primordial germ cells. Organogenesis (IF 2.321) Pub Date : 2017-05-30 Zograb Makiyan
Endometriosis is defined by the presence of endometrial ectopia. Multiple hypotheses have been postulated to explain the etiology of endometriosis to understand various clinical evidences. The etiology of endometriosis is still unclear.The primary question to understanding the etiology of endometrial ectopia (endometriosis) is determining the origin of eutopic (normally cited) endometrium.According
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Microenvironment of a tumor-organoid system enhances hepatocellular carcinoma malignancy-related hallmarks. Organogenesis (IF 2.321) Pub Date : 2017-05-26 Yang Wang,Kazuki Takeishi,Zhao Li,Eduardo Cervantes-Alvarez,Alexandra Collin de l'Hortet,Jorge Guzman-Lepe,Xiao Cui,Jiye Zhu
Organ-like microenviroment and 3-dimensional (3D) cell culture conformations have been suggested as promising approaches to mimic in a micro-scale a whole organ cellular functions and interactions present in vivo. We have used this approach to examine biologic features of hepatocellular carcinoma (HCC) cells. In this study, we demonstrate that hepatocellular carcinoma (HCC) cells, fibroblasts, endothelial
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Analysis of spatiotemporal pattern and quantification of gastrointestinal slow waves caused by anticholinergic drugs. Organogenesis (IF 2.321) Pub Date : 2017-03-15 Kelvin K L Wong,Lauren C Y Tang,Jerry Zhou,Vincent Ho
Anticholinergic drugs are well-known to cause adverse effects, such as constipation, but their effects on baseline contractile activity in the gut driven by slow waves is not well established. In a video-based gastrointestinal motility monitoring (GIMM) system, a mouse's small intestine was placed in Krebs solution and recorded using a high definition camera. Untreated controls were recorded for each
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Comparison of early vascular morphological changes between bioresorbable poly-L-lactic acid scaffolds and metallic stents in porcine iliac arteries. Organogenesis (IF 2.321) Pub Date : 2017-02-08 Yasuhito Sekimoto,Hideaki Obara,Kentaro Matsubara,Naoki Fujimura,Hirohisa Harada,Yuko Kitagawa
Bioresorbable scaffolds have the potential to overcome several problems associated with metallic stents. Bioresorbable poly-L-lactic acid (PLLA) scaffold implantation for the treatment of peripheral artery disease has already been reported in animal models and clinical trials; however, no studies comparing PLLA scaffolds and bare metal stents (BMSs) with regard to early vascular morphological changes
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Current strategies to generate mature human induced pluripotent stem cells derived cholangiocytes and future applications. Organogenesis (IF 2.321) Pub Date : 2017-01-25 Eduardo Cervantes-Alvarez,Yang Wang,Alexandra Collin de l'Hortet,Jorge Guzman-Lepe,Jiye Zhu,Kazuki Takeishi
Stem cell research has significantly evolved over the last few years, allowing the differentiation of pluripotent cells into almost any kind of lineage possible. Studies that focus on the liver have considerably taken a leap into this novel technology, and hepatocyte-like cells are being generated that are close to resembling actual hepatocytes both genotypically and phenotypically. The potential of
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Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold. Organogenesis (IF 2.321) Pub Date : 2017-01-19 Wessam Hassanein,Mehmet C Uluer,John Langford,Jhade D Woodall,Arielle Cimeno,Urmil Dhru,Avraham Werdesheim,Joshua Harrison,Carlos Rivera-Pratt,Stephen Klepfer,Ali Khalifeh,Bryan Buckingham,Philip S Brazio,Dawn Parsell,Charlie Klassen,Cinthia Drachenberg,Rolf N Barth,John C LaMattina
Recent years have seen a proliferation of methods leading to successful organ decellularization. In this experiment we examine the feasibility of a decellularized liver construct to support growth of functional multilineage cells. Bio-chamber systems were used to perfuse adult rat livers with 0.1% SDS for 24 hours yielding decellularized liver scaffolds. Initially, we recellularized liver scaffolds
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Par-1b is required for morphogenesis and differentiation of myoepithelial cells during salivary gland development. Organogenesis (IF 2.321) Pub Date : 2016-11-23 Elise M Gervais,Sharon J Sequeira,Weihao Wang,Stanley Abraham,Janice H Kim,Daniel Leonard,Kara A DeSantis,Melinda Larsen
The salivary epithelium initiates as a solid mass of epithelial cells that are organized into a primary bud that undergoes morphogenesis and differentiation to yield bilayered acini consisting of interior secretory acinar cells that are surrounded by contractile myoepithelial cells in mature salivary glands. How the primary bud transitions into acini has not been previously documented. We document
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