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Gene-expression profile and postpartum transition of bovine endometrial side population cells† Biol. Reprod. (IF 3.322) Pub Date : 2021-01-13 Ryoki Tatebayashi; Sho Nakamura; Shiori Minabe; Tadashi Furusawa; Ryoya Abe; Miki Kajisa; Yasuhiro Morita; Satoshi Ohkura; Koji Kimura; Shuichi Matsuyama
The mechanism of bovine endometrial regeneration after parturition remains unclear. Here, we hypothesized that bovine endometrial stem/progenitor cells participate in the postpartum regeneration of the endometrium. Flow cytometry analysis identified the presence of side population (SP) cells among endometrial stromal cells. Endometrial SP cells were shown to differentiate into osteoblasts and adipocytes
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Differences in the proteome of stallion spermatozoa explain stallion-to-stallion variability in sperm quality post thaw† Biol. Reprod. (IF 3.322) Pub Date : 2021-01-12 Gemma Gaitskell-Phillips; Francisco E Martín-Cano; José M Ortiz-Rodríguez; Antonio Silva-Rodríguez; Maria C Gil; Cristina Ortega-Ferrusola; Fernando J Peña
Although cryopreservation is becoming a common practice in modern equine breeding, there are still a number of unresolved issues limiting its use. One of these is the stallion to stallion variability in the post thaw quality of ejaculates. The identification of stallions and or ejaculates that will provide commercially acceptable quality post thaw, before cryopreservation is of great interest, avoiding
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Protein Hormone Fragmentation in Intercellular Signaling: Hormones as Nested Information Systems† Biol. Reprod. (IF 3.322) Pub Date : 2021-01-05 Kenneth L Campbell; Nurit Haspel; Cassandra Gath; Nuzulul Kurniatash; Indira (Nouduri) Akkiraju; Naomi Stuffers; Uma Vadher
This study explores the hypothesis that protein hormones are nested information systems in which initial products of gene transcription, and their subsequent protein fragments, before and after secretion and initial target cell action, play additional physiological regulatory roles. The study produced four tools and key results: 1) a problem approach that proceeds, with examples and suggestions for
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Male-derived copulatory plugs enhance implantation success in female Mus musculus† Biol. Reprod. (IF 3.322) Pub Date : 2020-12-23 Lough-Stevens Michael; Caleb Ghione; Matthew Urness; Adelaide Hobbs; Colleen Sweeney; Matthew D Dean
Among a wide diversity of sexually reproducing species, male ejaculates coagulate to form what has been termed a copulatory plug. A number of functions have been attributed to copulatory plugs, including, but not limited to, the inhibition of female remating and the promotion of ejaculate movement. Here we demonstrate that copulatory plugs also influence the likelihood of implantation, which occurs
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Synergistic action of estradiol and PGE2 on endometrial transcriptome in vivo resembles pregnancy effects better than estradiol alone† Biol. Reprod. (IF 3.322) Pub Date : 2020-12-22 Piotr Kaczynski; Stefan Bauersachs; Ewelina Goryszewska; Monika Baryla; Agnieszka Waclawik
Successful pregnancy establishment in mammals depends on numerous interactions between embryos and the maternal organism. Estradiol-17β (E2) is the primary embryonic signal in the pig, and its importance has been questioned recently. However, E2 is not the only molecule of embryonic origin. In pigs, prostaglandin E2 (PGE2) is abundantly synthesized and secreted by conceptuses and endometrium. The present
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Implantation failure and embryo loss contribute to subfertility in female mice mutant for chromatin remodeler Cecr2† Biol. Reprod. (IF 3.322) Pub Date : 2020-12-22 Kacie A Norton; Farshad Niri; Chelsey B Weatherill; Christine E Williams; Kevin Duong; Heather E McDermid
Defects in the maternal reproductive system that result in early pregnancy loss are important causes of human female infertility. A wide variety of biological processes are involved in implantation and establishment of a successful pregnancy. While chromatin remodelers have been shown to play an important role in many biological processes, our understanding of the role of chromatin remodelers in female
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Comprehensive spatiotemporal DNA methylation analysis of mouse tissue and organ progression through fetal development to adulthood†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-09 Elizabeth Elder,Serge McGraw
The precise and timely expression of genes, starting as soon as initial fertilization and continuously throughout fetal tissue progression, is crucial to ensure the proper development of mammalian embryos [1]. The synergy between epigenetic modifications and the binding of specific transcription factors (TFs) to gene regulatory elements is primarily responsible for coordinating this dynamic spatiotemporal
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CRISPR/CAS9-mediated amino acid substitution reveals phosphorylation residues of RSPH6A are not essential for male fertility in mice†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-09 Haruhiko Miyata,Ferheen Abbasi,Pablo E Visconti,Masahito Ikawa
genome editingradial spokesperm capacitationsperm motility
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Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-01 Aline Bueno,Yuri Karen Sinzato,Gustavo Tadeu Volpato,Franciane Quintanilha Gallego,Felipe Perecin,Tiago Rodrigues,Débora Cristina Damasceno
AbstractPreexisting/pregestational diabetes enhances the risk of birth defects. Several factors have been involved during the implantation process, such as cytokines (granulocyte-macrophage–colony-stimulating factor [GM-CSF]). The objective was to evaluate the effects of two levels of diabetes on the redox status of preimplantation embryos during the implantation process to comprehend how both are
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The impact of transcription inhibition during in vitro maturation on the proteome of bovine oocytes. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-28 Katrin Gegenfurtner,Florian Flenkenthaler,Thomas Fröhlich,Eckhard Wolf,Georg J Arnold
AbstractProper oocyte maturation is a prerequisite for successful reproduction and requires the resumption of meiosis to the metaphase II stage (MII). In bovine oocytes, nuclear maturation has been shown to occur in in vitro maturing cumulus-enclosed oocytes (COCs) in the absence of transcription, but their developmental capacity is reduced compared to transcriptionally competent COCs. To assess the
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Roles of Aryl Hydrocarbon Receptor in Endothelial Angiogenic Responses†. Biol. Reprod. (IF 3.322) Pub Date : 2020-07-27 Yan Li,Chi Zhou,Wei Lei,Kai Wang,Jing Zheng
AbstractAryl hydrocarbon receptor (AhR) is a transcription factor, which can be activated by a plethora of structure-diverse ligands. Historically, AhR is known for its involvements in regulation of metabolism of xenobiotics. However, normal physiological roles of AhR have been defined in other essential biological processes, including vascular growth and function, reproduction, and immunoresponses
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Pig Conceptuses Secrete Interferon Gamma to Recruit T cells to the Endometrium during the Peri-Implantation Period. Biol. Reprod. (IF 3.322) Pub Date : 2020-07-27 Bryan A McLendon,Heewon Seo,Avery C Kramer,Robert C Burghardt,Fuller W Bazer,Greg A Johnson
AbstractThe emerging paradigm in the immunology of pregnancy is that implantation of conceptuses does not progress in an immunologically suppressed environment. Rather, the endometrium undergoes a controlled inflammatory response during implantation as trophectoderm of elongating and implanting pig conceptuses secrete the pro-inflammatory cytokine interferon gamma (IFNG). Results of this study with
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The rapamycin analog Everolimus reversibly impairs male germ cell differentiation and fertility in the mouse1. Biol. Reprod. (IF 3.322) Pub Date : 2020-07-27 Oleksandr Kirsanov,Randall H Renegar,Jonathan T Busada,Nicholas D Serra,Ellen V Harrington,Taylor A Johnson,Christopher B Geyer
AbstractSirolimus, also known as rapamycin, and its closely related rapamycin analog (rapalog) Everolimus inhibit “mammalian target of rapamycin complex 1” (mTORC1), whose activity is required for spermatogenesis. Everolimus is Food and Drug Administration approved for treating human patients to slow growth of aggressive cancers and preventing organ transplant rejection. Here, we test the hypothesis
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Distinct changes of in BTLA, ICOS, PD-1 and TIGIT expression on peripheral blood and decidual CD8+ T cells in women with unexplained recurrent abortion. Biol. Reprod. (IF 3.322) Pub Date : 2020-07-24 Qianqian Liang,Lingxia Tong,Liping Xiang,Sujuan Shen,Chenhuan Pan,Cuiping Liu,Hong Zhang
AbstractThe two-way communication between the mother and the fetus is accomplished by immune cells. CD8+ T cells of normal pregnant (NP) women express progesterone receptor (PR). Binding of PR to progesterone (P) and the production of progesterone-induced blocking factor (PIBF) can aid immune escape, which is an important factor in the maternal immune response. We detected the proportion of CD8+ T
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An interview with Professor Ilpo Huhtaniemi Biol. Reprod. (IF 3.322) Pub Date : 2020-07-06 Kumar T.
Dr. Ilpo Huhtaniemi is Professor Emeritus of Reproductive Endocrinology at the Imperial College London, UK, Professor Emeritus of Physiology at the University of Turku, Finland and Visiting Professor at the University of Lund, Sweden. He obtained his MD and PhD degrees from University of Helsinki, Finland. During 1975–76, he was a postdoctoral fellow in the Department of Obstetrics and Gynecology and
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Transcriptome analysis of porcine endometrium after LPS-induced inflammation: effects of the PPAR-gamma ligands in vitro† Biol. Reprod. (IF 3.322) Pub Date : 2020-10-28 Mierzejewski K, Paukszto Ł, Kurzyńska A, et al.
AbstractFemale fertility depends greatly on the capacity of the uterus to recognize and eliminate microbial infections, a major reason of inflammation in the endometrium in many species. This study aimed to determine the in vitro effect of peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the transcriptome genes expression and alternative splicing in the porcine endometrium in the
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Conditional surrender in one generation: determining the reproductive roles of mouse embryo lethal genes by embryo complementation Biol. Reprod. (IF 3.322) Pub Date : 2020-10-15 John C Schimenti
The laboratory mouse is the most widely used animal model for studying the genetics and biology of mammalian development and reproduction. Embryonic stem cell (ESC) gene targeting technology, and the sophisticated genomic manipulations it allowed, was unique to this organism for a long period of time; this was a major factor in the mouse’s rise to pre-eminence as a model system over the past three
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Maternal obesity alters placental lysophosphatidylcholines, lipid storage, and the expression of genes associated with lipid metabolism‡ Biol. Reprod. (IF 3.322) Pub Date : 2020-10-13 Katie L Bidne; Alana L Rister; Andrea R McCain; Brianna D Hitt; Eric D Dodds; Jennifer R Wood
Dyslipidemia is a characteristic of maternal obesity and previous studies have demonstrated abnormalities in fatty acid oxidation and storage in term placentas. However, there is little information about the effect of pre-pregnancy obesity on placental lipid metabolism during early pregnancy. The objective of this study was to determine the relationship between lipid profiles and markers of metabolism
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Lipopolysaccharide-induced epididymitis modifies the transcriptional profile of Wfdc genes in mice Biol. Reprod. (IF 3.322) Pub Date : 2020-10-09 Alexandre D Andrade; Priscila G C Almeida; Noemia A P Mariani; Geanne A Freitas; Hélio Kushima; André L Filadelpho; Maria Angélica Spadella; Maria Christina W Avellar; Erick J R Silva
Whey-acidic protein four-disulfide core domain (WFDC) genes display putative roles in innate immunity and fertility. In mice, a locus on chromosome 2 contains 5 and 11 Wfdc genes in its centromeric and telomeric subloci, respectively. Although Wfdc genes are highly expressed in the epididymis, their contributions to epididymal function remain elusive. Here, we investigated whether Wfdc genes are regulated
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NEDD4-like ubiquitin ligase 2 protein (NEDL2) in porcine spermatozoa, oocytes, and preimplantation embryos and its role in oocyte fertilization Biol. Reprod. (IF 3.322) Pub Date : 2020-10-08 Jiude Mao; Michal Zigo; Dalen Zuidema; Miriam Sutovsky; Peter Sutovsky
The ubiquitin-proteasome system plays diverse regulatory and homeostatic roles in mammalian reproduction. Ubiquitin ligases are the substrate-specific mediators of ubiquitin-binding to its substrate proteins. The NEDD4-like ubiquitin ligase 2 (aliases NEDL2, HECW2) is a HECT-type ubiquitin ligase that contains one N-terminal HECW ubiquitin ligase domain, one C-terminal HECT ubiquitin ligase domain
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Erratum to 'The postcoital test in the development of new vaginal contraceptives'. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-02 Christine K Mauck,Kathleen L Vincent
In the original version of the article, a correction in Table 2 was inadvertently missed. The text for the ACIDFORM gel previously read as “13.7% [42] (n=1183) (7-cycle cumulative typical-use pregnancy rate or Food and Drug Administration (FDA) reviewed. Amphora was applied 0–60 min before coitus.)”. However, the correct text is “13.7% [42] (n=1183) (7-cycle cumulative typical-use pregnancy rate).
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Corrigendum: Experimentally Induced Endometritis Impairs the Developmental Capacity of Bovine Oocytes†. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-06 Mackenzie J Dickson,Rachel L Piersanti,Rosabel Ramirez-Hernandez,Eduardo Barros de Oliveira,Jeanette V Bishop,Thomas R Hansen,Zhengxin Ma,Kwang Cheol C Jeong,Jose E P Santos,Martin I Sheldon,Jeremy Block,John J Bromfield
The strain of Trueperella pyogenes described in the original manuscript is incorrect and should read T. pyogenes MS249. The MS249 strain was the only T. pyogenes used throughout this study. Intrauterine infusion of E. coli MS499 and T. pyogenes MS249 was used to induce uterine infection in cattle.
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An interview with Dr. Jerome F. Strauss III Biol. Reprod. (IF 3.322) Pub Date : 2020-10-03 Wei Yan
Jerome F. Strauss, III, M.D., Ph.D. completed college at the Brown University and received the MD and PhD degree from University of Pennsylvania. He is currently Professor of Obstetrics and Gynecology at the Virginia Commonwealth University School of Medicine, and emeritus Professor of Obstetrics and Gynecology at the University of Pennsylvania, where he was founding director of the Center for Research
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Lipid metabolism is altered in maternal, placental, and fetal tissues of ewes with small for gestational age fetuses Biol. Reprod. (IF 3.322) Pub Date : 2020-10-01 Chelsie B Steinhauser; Katharine Askelson; Colleen A Lambo; Kenneth C Hobbs; Fuller W Bazer; M Carey Satterfield
Nutrient restriction (NR) has the potential to negatively impact birthweight, an indicator of neonatal survival and lifelong health. Those fetuses are termed as small for gestational age (SGA). Interestingly, there is a spectral phenotype of fetal growth rates in response to NR associated with changes in placental development, nutrient and waste transport, and lipid metabolism. A sheep model with a
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Dynamics of paternal contributions to early embryo development in large animals Biol. Reprod. (IF 3.322) Pub Date : 2020-09-30 Bradford W Daigneault
This review focuses on current knowledge of paternal contributions to preimplantation embryonic development with particular emphasis on large animals. Specifically, the included content aims to summarize genomic and epigenomic contributions of paternally expressed genes, their regulation and chromatin structure that are indispensable for early embryo development. The accumulation of current knowledge
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Altered expression of ADM and ADM2 by hypoxia regulates migration of trophoblast and HLA-G expression† Biol. Reprod. (IF 3.322) Pub Date : 2020-09-30 Changdai Gu; Sohae Park; Jin Seok; Hee Yeon Jang; Yong Ju Bang; G I Jin Kim
Preeclampsia (PE) is a placental disorder caused by endothelial dysfunction via trophoblast inadequate invasion activity. Adrenomedullin (ADM) and ADM2 are multifunctional peptides that can support vascular activity and placental growth. However, correlation between ADMs and trophoblast functions is currently unclear. The objective of this study was to analyze changes in expression of ADMs in placenta
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Pleiotropic role of prokineticin 1 in the porcine endometrium during pregnancy establishment and embryo implantation † Biol. Reprod. (IF 3.322) Pub Date : 2020-09-30 Ewelina Goryszewska; Piotr Kaczynski; Monika Baryla; Agnieszka Waclawik
Acquisition of endometrial receptivity for embryo implantation is one of the crucial processes during pregnancy and is induced mainly by progesterone and enhanced by conceptus signals. Prokineticin 1 (PROK1) is characterized as a secretory protein with diverse functions in various tissues, including the reproductive tract. PROK1, with its receptor PROKR1, are up-regulated in the porcine endometrium
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Dux facilitates post-implantation development, but is not essential for zygotic genome activation Biol. Reprod. (IF 3.322) Pub Date : 2020-09-30 Darko Bosnakovski; Micah D Gearhart; Si Ho Choi; Michael Kyba
Double homeobox genes are unique to eutherian mammals. It has been proposed that the DUXC clade of the double homeobox gene family, which is present in multicopy long tandem arrays, plays an essential role in zygotic genome activation (ZGA). We generated a deletion of the tandem array encoding the DUXC gene of mouse, Double homeobox (Dux), and found it surprisingly to be homozygous viable and fertile
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Development of assisted reproductive technologies for Mus spretus Biol. Reprod. (IF 3.322) Pub Date : 2020-09-29 Ayumi Hasegawa; Keiji Mochida; Shogo Matoba; Kimiko Inoue; Daiki Hama; Masayo Kadota; Noriko Hiraiwa; Atsushi Yoshiki; Atsuo Ogura
The genus Mus consists of many species with high genetic diversity. However, only one species, Mus musculus (the laboratory mouse), is common in biomedical research. The unavailability of assisted reproductive technologies (ARTs) for other Mus species might be a major reason for their limited use in laboratories. Here, we devised ARTs for Mus spretus (the Algerian mouse), a commonly used wild-derived
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Necessity is the mother of invention and the evolutionary force driving the success of in vitro fertilization. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-25 Alison F Bartolucci,John J Peluso
This special issue of Biology of Reproduction, focused on contraception, was galvanized by the annual Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD). Contraceptive Development Meeting that was hosted by Baylor College of Medicine in Houston, Texas. The timing of the meeting (November 3–6, 2019) and the freedom that we had to interact and socialize was fortuitous
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Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-23 Kento Miura,Shogo Matoba,Michiko Hirose,Atsuo Ogura
Conditional knockout (cKO) mice have contributed greatly to understanding the tissue- or stage-specific functions of genes in vivo. However, the current cKO method requires considerable time and effort because of the need to generate two gene-modified mouse strains (Cre transgenic and loxP knockin) for crossing. Here, we examined whether we could analyze the germ cell-related functions of embryonic
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Cytoplasmic maturation in human oocytes: an ultrastructural study † Biol. Reprod. (IF 3.322) Pub Date : 2020-09-21 Z Trebichalská; D Kyjovská; S Kloudová; P Otevřel; A Hampl; Z Holubcová
Female fertility relies on successful egg development. Besides chromosome segregation, complex structural and biochemical changes in the cytoplasmic compartment are necessary to confer the female gamete the capacity to undergo normal fertilization and sustain embryonic development. Despite the profound impact on egg quality, morphological bases of cytoplasmic maturation remain largely unknown. Here
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Hyaluronidase-based swim-up for semen selection in patients with human papillomavirus semen infection Biol. Reprod. (IF 3.322) Pub Date : 2020-09-21 Luca De Toni; Ilaria Cosci; Andrea Carosso; Luisa Barzon; Bruno Engl; Carlo Foresta; Andrea Garolla
Infection by human papillomavirus (HPV) represents one of the most common sexually transmitted diseases in both men and women worldwide. Recently, the detection of HPV virions in the semen of a large percentage of sexually active men has been associated with detrimental effects on both sperm parameters and on assisted reproductive technologies (ART) treatment outcomes. Conventional semen washing procedure
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Actinomycin D causes oocyte maturation failure by inhibiting chromosome separation and spindle assembly Biol. Reprod. (IF 3.322) Pub Date : 2020-09-21 Tianjie Li; Changyu Liu; Xiumei Zhen; Yang Yu; Jie Qiao
Actinomycin D (ActD) has been considered as one of the most effective and safe chemotherapeutic medications for treating a number of cancers. Although ActD has been used in the treatment of gynecological tumors and pediatric tumors for more than 50 years, the toxic effects of ActD on mammalian oocytes remain unknown. In this study, the influence of ActD on mouse and human oocyte maturation and the
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The actions of resveratrol in decidualizing endometrium: acceleration or inhibition?† Biol. Reprod. (IF 3.322) Pub Date : 2020-09-21 Kuroda K, Ochiai A, Brosens J.
AbstractResveratrol, a natural polyphenolic compound, is widely studied for its anti-inflammatory and antisenescent properties. Recently, two studies reported seemingly conflicting findings on the actions of resveratrol on decidualization of human endometrial stromal cells (HESCs). One study by Ochiai et al. demonstrated that resveratrol inhibits decidual transformation of primary cultured HESCs. The
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Bisphenol A and 17α-ethinylestradiol-induced Transgenerational Gene Expression Differences in the Brain-Pituitary-Testis Axis of Medaka, Oryzias latipes. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-17 Albert J Thayil,Xuegeng Wang,Pooja Bhandari,Frederick S Vom Saal,Donald E Tillitt,Ramji K Bhandari
Endocrine disrupting chemicals (EDCs), such as bisphenol A (BPA) and 17α-ethinylestradiol (EE2), can have far reaching health effects, including transgenerational abnormalities in offspring that never directly contacted either chemical. We previously reported reduced fertilization rates and embryo survival at F2 and F3 generations caused by 7-day embryonic exposure (F0) to 100 μg/L BPA or 0.05 μg/L
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Ephrin and Eph Receptor Signaling in Female Reproductive Physiology and Pathology. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-17 Enoch Appiah Adu-Gyamfi,Armin Czika,Tai-Hang Liu,Philip Narteh Gorleku,Linda Ahenkorah Fondjo,Francis Tanam Djankpa,Yu-Bin Ding,Ying-Xiong Wang
Ephrins are ligands of Eph receptors (Ephs); both of which are sorted into two classes, A and B. There are five types of ephrin-As (ephrin-A1–5) and three types of ephrin-Bs (ephrin-B1–3). Also, there are 10 types of EphAs (EphA1–10) and six types of EphBs (EphB1–6). Binding of ephrins to the Eph receptors activates signaling cascades that regulate several biological processes such as cellular proliferation
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Selection of fewer dominant follicles in Trio carriers given GnRH antagonist and LH action replaced by nonpulsatile hCG.†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-17 Victor E Gomez-León,João Paulo Andrade,Brian W Kirkpatrick,Sadrollah Molaei Moghbeli,Alvaro García-Guerra,O J Ginther,Milo C Wiltbank
Studying selection of multiple dominant follicles (DFs) in monovulatory species can advance our understanding of mechanisms regulating selection of single or multiple DFs. Carriers of the bovine high fecundity Trio allele select multiple DFs, whereas half-sib noncarriers select a single DF. This study compared follicle selection during endogenous gonadotropin pulses versus during ablation of pulses
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Production of Donor-Derived Eggs After Ovarian Germ-Cell Transplantation into the Gonads of Adult, Germ Cell-Less, Triploid Hybrid Fish†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-17 Dongdong Xu,Tasuku Yoshino,Marcelo Bello Cioffi,Hiroyuki Yoshikawa,Yasuko Ino,Ryosuke Yazawa,Samyra Maria Santos Nassif Lacerda,Yutaka Takeuchi
In animals, spermatogonial transplantation in sterile adult males is widely developed; however, despite its utility, ovarian germ cell transplantation is not well developed. We previously showed that the interspecific hybrid offspring of sciaenid was a suitable model for germ cell transplantation studies as they have germ cell-less gonads. However, all these gonads have testis-like characteristics
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Progesterone receptor membrane component 1 is required for mammary gland development. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-11 Globinna Kim,Jong Geol Lee,Seung-A Cheong,Jung-Min Yon,Myeong Sup Lee,Eui-Ju Hong,In-Jeoung Baek
The physiological functions of progesterone (P4) in female reproductive organs including the mammary glands are mediated via the progesterone receptor (PR), but not all P4 functions can be explained by PR-mediated signaling. Progesterone receptor membrane component 1 (PGRMC1), a potential mediator of P4 actions, plays an important role in the ovary and uterus in maintaining female fertility and pregnancy
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Transgenic F2 bovine embryos show stable germline transmission and maintenance of transgene expression through two generations. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-11 Gyeong-Min Gim,Dong-Hyeok Kwon,Won-Wu Lee,Dae-Jin Jung,Dae-Hyun Kim,Jun-Koo Yi,Goo Jang
Production of transgenic animal models with a fluorescence (i.e., green fluorescent protein; GFP) gene has been considered an important resource because such models allow real-time tracing of cellular differentiation and function [1]. Thus, several fluorescent models including mice, rats, and rabbits have been successfully developed and used widely in research. Fluorescent models in large animals,
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Maternal high fat diet-induced obesity affects trophoblast differentiation and placental function in mice. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-11 Tobias Kretschmer,Eva-Maria Turnwald,Ruth Janoschek,Peter Zentis,Inga Bae-Gartz,Tim van Beers,Marion Handwerk,Maria Wohlfarth,Mojgan Ghilav,Wilhelm Bloch,Eva Hucklenbruch-Rother,Jörg Dötsch,Sarah Appel
Evidence suggests that maternal obesity (MO) can aggravate placental function causing severe pathologies during the perinatal window. However, molecular changes and mechanisms of placental dysfunction remain largely unknown. This work aimed to decipher structural and molecular alterations of the placental transfer zone associated with MO. To this end, mice were fed a high fat diet (HFD) to induce obesity
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Acetylcholine Ameliorated TNF-α-induced Primary Trophoblast Malfunction via Muscarinic Receptors. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-09 Zheng Wang,Adoulaye Issotina Zibrila,Shuhua Liu,Gongxiao Zhao,Yubei Li,Jingning Xu,Dan Liu,Chunfang Li,Weiyi Feng,Jinjun Liu
Oxidative stress and apoptosis of trophoblasts are involved in preeclampsia (PE). Numerous studies have shown that acetylcholine (ACh), the principal vagal neurotransmitter, plays a crucial role in attenuating oxidative stress, inflammation, and apoptosis in a variety of human diseases. However, the role of ACh in PE management remains unclear. Here, we aimed to determine the effects of ACh on TNF-α-treated
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Role of DNA damage and repair mechanisms in uterine fibroid/leiomyomas: A review. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-09 Sneh M Toprani,Varsha Kelkar Mane
There has been a significant annual increase in the number of cases of uterine leiomyomas or fibroids (UF) among women of all races and ages across the world. A fortune is usually spent by the healthcare sector for fibroid-related treatments and management. Molecular studies have established the higher mutational heterogeneity in UF as compared to normal myometrial cells. The contribution of DNA damage
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Prostaglandin I2 Mediates Weak Vasodilatation in Human Placental Microvessels†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-09 Xueqin Feng,Yingying Zhang,Yumeng Zhang,Xiaojun Yang,Dongmei Man,Likui Lu,Ting Xu,Yanping Liu,Chunli Yang,Huan Li,Linglu Qi,Hongyu Su,Xiuwen Zhou,Zhice Xu
Human placental vessels (HPVs) play important roles in the exchange of metabolites and oxygen in maternal-fetal circulation. Endothelial-derived prostacyclin (prostaglandin I2, PGI2) is a critical endothelial vasodilator in the body. However, the physiological and pharmacological functions of endothelial PGI2 in the human placenta are still unclear. Human, sheep, and rat blood vessels were used in
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Transforming growth factor beta signaling and decidual integrity in mice†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-09 Xin Fang,Nan Ni,Yang Gao,John P Lydon,Ivan Ivanov,Monique Rijnkels,Kayla J Bayless,Qinglei Li
Transforming growth factor beta (TGFβ) signaling regulates multifaceted reproductive processes. It has been shown that the type 1 receptor of TGFβ (TGFBR1) is indispensable for female reproductive tract development, implantation, placental development, and fertility. However, the role of TGFβ signaling in decidual development and function remains poorly defined. Our objective is to determine the impact
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An interview with Dr Bernard Robaire, recipient of the 2020 SSR Jansen Distinguished Leadership and Service Award Biol. Reprod. (IF 3.322) Pub Date : 2020-09-09 Zelieann R Craig
Bernard Robaire, received his BA from University of California, Los Angeles and his PhD from McGill University. After a postdoctoral fellowship at Johns Hopkins University, he returned to McGill to take up a joint appointment in the Departments of Pharmacology and Therapeutics and of Obstetrics and Gynecology where he has remained and is currently a James McGill Professor. Dr Robaire’s research interests
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ORP9 Knockdown Delays the Maturation of Junction Related Endocytic Structures in the Testis and Leads to Impaired Sperm Release†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-09 Arlo Adams,Wayne Vogl
The release of late spermatids from the seminiferous epithelium requires the internalization of intercellular junctions by Sertoli cell specific structures called “tubulobulbar complexes” (TBCs). These large, endocytic devices likely evolved from classic clathrin-mediated-endocytosis (CME) machinery, but have several important morphological differences to CME vesicles. Most notable among these differences
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Lack of effect of short-term DHEA supplementation on the perimenopause ovary†. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-09 Selva L Luna,Donald I Brown,Steven G Kohama,Henryk F Urbanski
Dehydroepiandrosterone (DHEA) hormonal supplementation can improve oocyte quality in women with diminished ovarian function. However, it is unclear whether DHEA supplementation can also enhance ovarian function during the perimenopause (i.e., when the number of follicles in the ovary has undergone a marked reduction). To address this question, we examined the impact of 2.5-months of daily 5-mg oral
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FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture. Biol. Reprod. (IF 3.322) Pub Date : 2020-09-04 Yi-Han Li,Tsung-Ming Chen,Bu-Miin Huang,Shang-Hsun Yang,Chia-Ching Wu,Yung-Ming Lin,Jih-Ing Chuang,Shaw-Jenq Tsai,H Sunny Sun
Fibroblast growth factor 9 (FGF9) is an autocrine/paracrine growth factor that plays critical roles in embryonic and organ developments and is involved in diverse physiological events. Loss of function of FGF9 exhibits male-to-female sex reversal in the transgenic mouse model and gain of FGF9 copy number was found in human 46, XX sex reversal patient with disorders of sex development. These results
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Seminal plasma AnnexinA2 protein is a relevant biomarker for stallions which require removal of seminal plasma for sperm survival upon refrigeration. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-28 Gemma Gaitskell-Phillips,Francisco E Martín-Cano,José M Ortiz-Rodríguez,Antonio Silva-Rodríguez,Heriberto Rodríguez-Martínez,Maria C Gil,Cristina Ortega-Ferrusola,Fernando J Peña
Some stallions yield ejaculates that do not tolerate conservation by refrigeration prior to artificial insemination (AI), showing improvement after removal of most of the seminal plasma (SP) by centrifugation. In this study, the SP-proteome of 10 different stallions was defined through high-performance liquid chromatography with tandem mass spectrometry and bioinformatic analysis in relation to the
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The promise of placental extracellular vesicles: models and challenges for diagnosing placental dysfunction in utero. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-28 Lindsey N Block,Brittany D Bowman,Jenna Kropp Schmidt,Logan T Keding,Aleksander K Stanic,Thaddeus G Golos
Monitoring the health of a pregnancy is of utmost importance to both the fetus and the mother. The diagnosis of pregnancy complications typically occurs after the manifestation of symptoms, and limited preventative measures or effective treatments are available. Traditionally, pregnancy health is evaluated by analyzing maternal serum hormone levels, genetic testing, ultrasonographic imaging, and monitoring
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The endometrial proteomic profile around the time of embryo implantation. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-28 Xi Guo,Tin Chiu Li,Xiaoyan Chen
Embryo implantation is an intricate process which requires competent embryo and receptive endometrium. The failure of endometrium to achieve receptivity is a recognized cause of infertility. However, due to multiplicity of events involved, the molecular mechanisms governing endometrial receptivity are still not fully understood. Traditional one-by-one approaches, including western blotting and histochemistry
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An Interview with Dr Joy Pate. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-25 Romana A Nowak
Dr Joy Pate is a Professor of Animal Sciences, the C. Lee Rumberger and Family Chair in Agricultural Sciences, and the Director of the Center for Reproductive Biology and Health at Penn State University. She received the BS degree in animal science from the University of Delaware and the PhD in animal sciences, with emphasis on reproductive biology, from the University of New Hampshire in the laboratory
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Structure of the reproductive system of the sexual generation of the endemic Arctic species Acyrthosiphon svalbardicum and its temperate counterpart Acyrthosiphon pisum (Hemiptera, Aphididae) Biol. Reprod. (IF 3.322) Pub Date : 2020-08-25 Karina Wieczorek; Dominik Chłond; Łukasz Junkiert; Piotr Świątek
The Arctic aphids live briefly and must breed quickly to survive. Shortened life cycle, with only two generations: the stem mother and sexuales—oviparous females and males is an adaptation for optimal use of the short breeding period, which lasts from late July to the end of August. Using Acyrthosiphon svalbardicum, an endemic High Arctic aphid species, we describe the structure of the reproductive
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Aberrant and constitutive expression of FOXL2 impairs ovarian development and functions in mice. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-25 Barbara Nicol,Karina Rodriguez,Humphrey H-C Yao
Development and functions of the ovary rely on appropriate signaling and communication between various ovarian cell types. FOXL2, a transcription factor that plays a key role at different stages of ovarian development, is associated with primary ovarian insufficiency and ovarian cancer as a result of its loss-of-function or mutations. In this study, we investigated the impact of aberrant, constitutive
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Increased FOXL2 expression alters uterine structures and functions†. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-25 Rong Li,San-Pin Wu,Lecong Zhou,Barbara Nicol,John P Lydon,Humphrey H-C Yao,Francesco J DeMayo
The transcription factor forkhead box L2 (FOXL2) regulates sex differentiation and reproductive function. Elevated levels of this transcription factor have been observed in the diseases of the uterus, such as endometriosis. However, the impact of elevated FOXL2 expression on uterine physiology remains unknown. In order to determine the consequences of altered FOXL2 in the female reproductive axis,
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Removal of peri-ovarian adipose tissue affects follicular development and lipid metabolism†. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-19 Menliang Zhu,Qiyang Shen,Xiaolian Li,Jihong Kang
The development and maturity of follicles are regulated by sex hormones and growth factors. It has been proven that peri-ovarian adipose tissue (POAT) plays an important role in folliculogenesis and fertility in the female ICR and KM mice. The aim of the present study was to further investigate whether the removal of bilateral POAT affected follicular development and lipid metabolism in the female
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Peripheral action of kisspeptin at reproductive tissues-role in ovarian function and embryo implantation and relevance to assisted reproductive technology in livestock: A review. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-10 Michael J D'Occhio,Giuseppe Campanile,Pietro S Baruselli
Kisspeptin (KISS1) is encoded by the KISS1 gene and was initially found to be a repressor of metastasis. Natural mutations in the KISS1 receptor gene (KISS1R) were subsequently shown to be associated with idiopathic hypothalamic hypogonadism and impaired puberty. This led to interest in the role of KISS1 in reproduction. It was established that KISS1 had a fundamental role in the control of gonadotropin
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C-ECi: A cubic-ECi combined clearing method for 3D follicular content analysis in The fish ovary†. Biol. Reprod. (IF 3.322) Pub Date : 2020-08-10 Manon Lesage,Manon Thomas,Jérôme Bugeon,Adèle Branthonne,Stéphanie Gay,Emilie Cardona,Marie Haghebaert,Fabrice Mahé,Julien Bobe,Violette Thermes