-
Development of a 3D spheroid cell culture system from fish cell lines for in vitro infection studies: Evaluation with Saprolegnia parasitica J. Fish Dis. (IF 2.318) Pub Date : 2021-01-12 Marc N. Faber; Jerry M. Sojan; Marcia Saraiva; Pieter van West; Christopher J. Secombes
Understanding the ways in which pathogens infect host cells is essential to improve and develop new treatment strategies. This study aimed to generate a novel in vitro infection model by establishing a reproducible 3D spheroid cell culture system that may lead to a reduced need for animals in fish disease research. 2D models (commonly cell lines) cannot replicate many key conditions of in vivo infections
-
Climate change‐driven disease in sympatric hosts: Temporal dynamics of parasite burden and proliferative kidney disease in wild brown trout and Atlantic salmon J. Fish Dis. (IF 2.318) Pub Date : 2021-01-11 Magnus Lauringson; Ilkka Nousiainen; Siim Kahar; Oksana Burimski; Riho Gross; Tanel Kaart; Anti Vasemägi
Global climate change is altering the abundance and spread of various parasites, which has important consequences not only for host–parasite interactions but also for the relationships between different host species. Here, we focus on the myxozoan endoparasite Tetracapsuloides bryosalmonae that causes temperature‐dependent proliferative kidney disease (PKD) in salmonids. We characterized the temporal
-
Review of inflammation in fish and value of the zebrafish model J. Fish Dis. (IF 2.318) Pub Date : 2020-11-25 Jose Carlos Campos‐Sánchez; María Ángeles Esteban
Inflammation is a crucial step in the development of chronic diseases in humans. Understanding the inflammation environment and its intrinsic mechanisms when it is produced by harmful stimuli may be a key element in the development of human disease diagnosis. In recent decades, zebrafish (Danio rerio) have been widely used in research, due to their exceptional characteristics, as a model of various
-
Case definitions for skin lesion syndromes in chinook salmon farmed in Marlborough Sounds, New Zealand J. Fish Dis. (IF 2.318) Pub Date : 2020-12-04 Hazel Johnston; Jane Symonds; Seumas Walker; Mark Preece; Cesar Lopez; Barbara Nowak
Skin lesions are commonly reported in farmed salmonids. Chinook salmon (Oncorhynchus tshawytscha), introduced from California USA, is the only salmonid species commercially farmed in New Zealand, although trout are cultured for release by Fish and Game New Zealand. There are several farming areas in New Zealand, including Marlborough Sounds, Canterbury and Stewart Island. While the industry has not
-
Immersion challenge of naïve Atlantic salmon with cultured Nolandella sp. and Pseudoparamoeba sp. did not increase the severity of Neoparamoeba perurans‐induced amoebic gill disease (AGD) J. Fish Dis. (IF 2.318) Pub Date : 2020-12-12 Chloe J. English; Natasha A. Botwright; Mark B. Adams; Andrew C. Barnes; James W. Wynne; Paula C. Lima; Mathew T. Cook
Amoebic gill disease (AGD) is one of the main health issues impacting farmed Atlantic salmon. Neoparamoeba perurans causes AGD; however, a diversity of other amoeba species colonizes the gills and there is little understanding of whether they are commensal or potentially involved in different stages of gill disease development. Here, we conduct in vivo challenges of naïve Atlantic salmon with cultured
-
Genetic analysis of resistance in Mekong striped catfish (Pangasianodon hypophthalmus) to bacillary necrosis caused by Edwardsiella ictaluri J. Fish Dis. (IF 2.318) Pub Date : 2020-11-20 Khoi Dinh Pham; Jørgen Ødegård; Sang Van Nguyen; Hans Magnus Gjøen; Gunnar Klemetsdal
The aim of this study was to analyse four cohabitation challenge‐test experiments with Mekong striped catfish (Pangasianodon hypophthalmus) against the bacterium Edwardsiella ictaluri. The data were genetically analysed per experiment by three models: 1) a cross‐sectional linear model; 2) a cross‐sectional threshold model; and 3) a linear survival model, at both 50% mortality (for models 1 and 2) and
-
Impact of feed restriction, chloroquine and deoxynivalenol on viral haemorrhagic septicaemia virus IVb in fathead minnow Pimephales promelas Rafinesque J. Fish Dis. (IF 2.318) Pub Date : 2020-11-09 Juan‐Ting Liu; John S. Lumsden
Autophagy can markedly alter host response to infectious disease, and several studies have demonstrated that a restricted diet or deoxynivalenol modulates autophagy and reduces mortality of fish due to bacterial disease. The picture is less clear for viral diseases of fish. Duplicate tanks of fathead minnow, Pimephales promelas Rafinesque, were fed a replete diet (control), 100 µM chloroquine, 5 µM
-
Transcriptome analysis of immune response against Siniperca chuatsi rhabdovirus infection in mandarin fish Siniperca chuatsi J. Fish Dis. (IF 2.318) Pub Date : 2021-01-10 Ying Huang; Ruixia Wang; Tianheng Gao; Ting Wu; Qiya Zhang; Yangbai Shi; Shuyan Ding; Zhe Zhao
As one of the piscine rhabdoviruses, Siniperca chuatsi rhabdovirus (SCRV) has caused considerable losses to mandarin fish aquaculture industry. RNA‐seq, as efficient transcriptome research method, has been widely used to study the immune response of fish to pathogens. This study reported the effect of SCRV infection at 0, 24 and 60 hr on S. chuatsi at the transcriptome level. A total of 61,527 unigenes
-
Expression and regulation of ccBAX by miR‐124 in the caudal fin cell of C. auratus gibelio upon cyprinid herpesvirus 2 infection J. Fish Dis. (IF 2.318) Pub Date : 2021-01-05 Lu Yu; Qikang Chen; Xin Chu; Yang Luo; Zizhao Feng; Liqun Lu; Ye Zhang; Dan Xu
Bcl2 family proteins play a critical role in cell death or survival. BAX, the death‐promoting protein of bcl2 family, mediated mitochondrial pathway inducing cells’ apoptosis in mammal. MiRNAs have been implicated as negative regulators down‐regulating genes’ expression after post‐transcriptional level. At present, little is known about the regulatory mechanism of miRNA on the Bcl2 family proteins
-
Skeletal deformities and meristic trait variations are common in the intertidal fish Bathygobius cocosensis (Perciformes‐Gobiidae) J. Fish Dis. (IF 2.318) Pub Date : 2021-01-05 Lucie Malard; Cynthia Riginos; Katrina McGuigan
Extreme environmental conditions, such as temperature, can lead to meristic trait variation and skeletal deformities, which may have major impacts on individual fitness. As intertidal ecosystems experience rapid temperature and physicochemical fluctuations, intertidal fish living and reproducing in these environmental conditions may have phenotypes influenced by such variable environments. The impact
-
Case of highly prevalent papilloma‐like skin lesions in whitespotted char (Salvelinus leucomaenis) in Kamchatka (Russia) J. Fish Dis. (IF 2.318) Pub Date : 2021-01-04 Natalia V. Sergeenko; Tatyana V. Gavruseva; Elena A. Ustimenko; Elena V. Bochkova; Tatyana V. Ryazanova
In October 2014, a case of mass skin lesions was reported in whitespotted char (Salvelinus leucomaenis) caught in the Nalycheva River on the Kamchatka peninsula (Russia). The proportion of affected fish was approximately 50% of the catch. Lesions of varying severity were noted from small tubercles to large single and confluent formations and ulcers. Papilloma‐like growths were found in the epidermal
-
Epizootic neoplasia in a managed population of Atlantic bumper fish, Chloroscombrus chrysurus (Osteichthyes: Carangidae), at a public aquarium J. Fish Dis. (IF 2.318) Pub Date : 2020-12-21 Justin M. Stilwell; Shane M. Boylan; Bryan Vorbach; Alvin C. Camus
Advances in fish medicine and husbandry have increased the average lifespans of specimens in managed aquarium populations. As a result, an increased incidence and variety of neoplasia is expected. This work characterizes diverse neoplasms arising within a managed population of Atlantic bumper fish acquired via repeated collections from the Charleston Harbor region. A total of 76 neoplasms were evaluated
-
Microbial pathogens of freshwater crayfish: A critical review and systematization of the existing data with directions for future research J. Fish Dis. (IF 2.318) Pub Date : 2020-12-20 Paula Dragičević; Ana Bielen; Ines Petrić; Sandra Hudina
Despite important ecological role and growing commercial value of freshwater crayfish, their diseases are underresearched and many studies examining potential crayfish pathogens do not thoroughly address their epizootiology, pathology or biology. This study reviews over 100 publications on potentially pathogenic viruses, bacteria, fungi and fungal‐like microorganisms reported in crayfish and systematizes
-
Molecular and functional identification of a β‐defensin homolog in large yellow croaker (Larimichthys crocea) J. Fish Dis. (IF 2.318) Pub Date : 2020-12-19 Kexin Li; Wanru Li; Xiaojuan Chen; Tian Luo; Yinnan Mu; Xinhua Chen
β‐defensin (BD) is a cysteine‐rich cationic antibacterial peptide that is active against a wide range of bacteria. Here, a β‐defensin homolog (LcBD2) was identified in large yellow croaker (Larimichthys crocea). The open reading frame of LcBD2 contains 195 nucleotides, encoding a protein of 64 amino acids that possesses a typical arrangement of six conserved cysteine residues (C31, C37, C41, C53, C59
-
Simultaneous detection of multiple bacterial and viral aquatic pathogens using a fluorogenic loop‐mediated isothermal amplification‐based dual‐sample microfluidic chip J. Fish Dis. (IF 2.318) Pub Date : 2020-12-19 Qian‐Jin Zhou; Jian‐Fei Lu; Xiu‐Rong Su; Jing‐Lei Jin; Shang‐Yang Li; Yan Zhou; Lei Wang; Xin‐Bin Shao; Yao‐Hua Wang; Mao‐Cang Yan; Ming‐Yun Li; Jiong Chen
Rapid and user‐friendly diagnostic tests are necessary for early diagnosis and immediate detection of diseases, particularly for on‐site screening of pathogenic microorganisms in aquaculture. In this study, we developed a dual‐sample microfluidic chip integrated with a real‐time fluorogenic loop‐mediated isothermal amplification assay (dual‐sample on‐chip LAMP) to simultaneously detect 10 pathogenic
-
Phaeohyphomycosis due to Veronaea botryosa in cultured white sturgeon (Acipenser transmontanus Richardson) from California USA during 2006 to 2015 J. Fish Dis. (IF 2.318) Pub Date : 2020-12-17 Joseph M. Groff; Mai Y. Mok; Steven V. Kubiski; Adam O. Michel; Galaxia A. Cortés‐Hinojosa; Barbara A. Byrne; Brian L. Wickes; E. Scott Weber; Lori A Campbell; Thomas B. Waltzek
Infection with Veronaea botryosa can result in rare cutaneous or disseminated, granulomatous to pyogranulomatous phaeohyphomycosis in humans, although disease due to the fungus has also been reported in non‐mammalian vertebrates. This report documents disease due to V. botryosa in captive, juvenile to subadult or young adult white sturgeon (Acipenser transmontanus Richardson) from California USA and
-
Genetic parameters of seven immune factors in turbot (Scophthalmus maximus) infected with Vibrio anguillarum J. Fish Dis. (IF 2.318) Pub Date : 2020-12-17 Xin‐An Wang; Ai‐Jun Ma; Zhi‐Bin Sun
In this study, 1,800 turbot (Scophthalmus maximus) individuals from 30 full‐sib families were experimentally infected with Vibrio anguillarum, and the expression levels of the immune factors lysozyme, hepcidin, heat‐shock protein 70 (HSP70), HSP90, immunoglobulin M (IgM), C‐type lectin and Lily‐type lectin in the liver were measured by real‐time PCR. Heritability values of the seven immune factors
-
Stability of viral haemorrhagic septicaemia virus, infectious hematopoietic necrosis virus and cyprinid herpesvirus 3 in various water samples J. Fish Dis. (IF 2.318) Pub Date : 2020-12-15 Johanna Ullrich; Jürgen Christian; Sven Michael Bergmann; Martin Oberle; Anna Maria Becker
Rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio) are the two most common species in traditional fish farming in Germany. Their aquaculture is threatened upon others by viruses that can cause a high mortality. Therefore, this work focuses on three viruses—viral haemorrhagic septicaemia virus, infectious hematopoietic necrosis virus and cyprinid herpesvirus 3 (CyHV‐3)—that endanger
-
Transcriptome analysis in Takifugu rubripes and Dicentrarchus labrax gills during Cryptocaryon irritans infection J. Fish Dis. (IF 2.318) Pub Date : 2020-12-13 Jian‐xin Cheng; Yu‐qing Xia; Ya‐fang Liu; Peng‐fei Liu; Ying Liu
Takifugu rubripes and Dicentrarchus labrax are important commercial fish in China that are under serious threat from Cryptocaryon irritans. C. irritans is a ciliated obligate parasite that causes marine white spot disease and leads to heavy economic losses. We analysed the transcriptome in the gills of T. rubripes and D. labrax to compare differentially expressed genes (DEGs) and pathways during infection
-
Development of a Piscirickettsia salmonis immersion challenge model to investigate the comparative susceptibility of three salmon species J. Fish Dis. (IF 2.318) Pub Date : 2020-10-16 Amy Long; Aidan Goodall; Simon R.M. Jones
Piscirickettsia salmonis, the aetiological agent of salmonid rickettsial septicaemia (SRS), is a global pathogen of wild and cultured marine salmonids. Here, we describe the development and application of a reproducible, standardized immersion challenge model to induce clinical SRS in juvenile pink (Oncorhynchus gorbuscha), Atlantic (Salmo salar) and sockeye salmon (O. nerka). Following a 1‐hr immersion
-
Comparative genomic analysis of different virulence strains reveals reasons for the increased virulence of Aeromonas veronii J. Fish Dis. (IF 2.318) Pub Date : 2020-11-02 Bintong Yang; Chong Chen; Yufeng Sun; Linan Cao; Dongxing Zhang; Wuwen Sun; Lei Zhang; Guiqin Wang; Xiaofeng Shan; Yuanhuan Kang; Aidong Qian
Aeromonas veronii is an important zoonotic and aquatic agent. More and more cases have shown that it has caused huge economic losses in the aquaculture industry in addition to threatening human health. But the reasons for the increasing virulence of A. veronii are still unclear. In order to further understand the reasons for the increased virulence of A. veronii, we conducted a comparative analysis
-
Variations in mucous cell numbers in gills of Atlantic salmon (Salmo salar) presmolt in commercial freshwater farms in Norway J. Fish Dis. (IF 2.318) Pub Date : 2020-10-18 David Persson; Håvard Bjørgen; Alexander Figenschou; Linn‐Anett Hillestad; Erling Olaf Koppang; Ane Nødtvedt; Marit Stormoen
Fish gills are heavily exposed to the external milieu and may react against irritants with different cellular responses. We describe variations in mucous cell counts in gills from healthy Atlantic salmon (Salmo salar) presmolts in five recirculating aquaculture system (RAS) farms and one flow‐through farm. Based on certain criteria, mucous cells were histologically quantified in a defined lamellar
-
Cytochrome oxidase gene sequencing reveals channel catfish ovary cell line is contaminated with brown bullhead cells J. Fish Dis. (IF 2.318) Pub Date : 2020-10-17 Lorelei Ford; Kuttichantran Subramaniam; Thomas B. Waltzek; Paul R. Bowser; Larry Hanson
The channel catfish (Ictalurus punctatus, Rafinesque) ovary (CCO) cell line is the standard cell line used for channel catfish diagnostics. Next‐gen sequencing studies of a virus cultured in the CCO cells revealed mitochondrial sequences matching those of brown bullhead (Ameiurus nebulosus, Lesueur). Therefore, we systematically performed partial cytochrome oxidase 1 gene sequencing of several sources
-
Infectious salmon anaemia virus detected by RT‐qPCR in Norwegian farmed rainbow trout, Oncorhynchus mykiss (Walbaum, 1792) J. Fish Dis. (IF 2.318) Pub Date : 2020-12-07 Marta Alarcón; Torfinn Moldal; Mona Dverdal Jansen; Maria Aamelfot; Hilde Sindre; Trude M. Lyngstad; Knut Falk
Infectious salmon anaemia (ISA), caused by infectious salmon anaemia virus (ISAV), is a notifiable infection of farmed Atlantic salmon, Salmo salar L. The disease is characterized by severe anaemia, infection of the circulatory system, bleeding and variable cumulative mortality. Natural outbreaks of ISA have only been observed in farmed Atlantic salmon, but ISAV has been detected from wild, apparently
-
A novel Dicistro‐like virus discovered in Procambarus clarkii with “Black May” disease J. Fish Dis. (IF 2.318) Pub Date : 2020-12-04 Pengdan Huang; Guoqing Shen; Jie Gong; Mengru Zhu; Yang Wang; Xiao Zhang; Kassimu Hashimu Ame; Yanan Zang; Huaishun Shen
The peak period of morbidity and death in cultured Procambarus clarkii is around May each year and is called the “Black May” disease. The pathogen causing “Black May” disease is believed to be a white spot syndrome virus (WSSV). In 2018, a significant number of P. clarkii died in the pond culture of Xinglong Township, Xuyi County. Two sampling tests on the affected pond showed that, in addition to
-
Novel atypical Aeromonas salmonicida bath challenge model for juvenile ballan wrasse (Labrus bergylta, Ascanius) J. Fish Dis. (IF 2.318) Pub Date : 2020-12-04 Athina Papadopoulou; Kathryn Garvey; Tom Hill; Jose G. Ramirez‐Paredes; Sean J. Monaghan; Johanna L. Baily; Andrew Davie; Ioanna Katsiadaki; David Verner‐Jeffreys; Timothy Wallis; Herve Migaud; Alexandra Adams
Atypical Aeromonas salmonicida (aAs) is currently one of the most routinely recovered bacterial pathogens isolated during disease outbreaks in farmed cleaner fish, ballan wrasse (Labrus bergylta, Ascanius). Vibrionaceae family bacteria have also been isolated from ballan wrasse in Scotland. This study determined the infectivity, pathogenicity and virulence of aAs and Vibrionaceae isolates in juvenile
-
Encapsulation of Anguillicola crassus reduces the abundance of adult parasite stages in the European eel (Anguilla anguilla) J. Fish Dis. (IF 2.318) Pub Date : 2020-12-03 Seraina E. Bracamonte; Klaus Knopf; Michael T. Monaghan
Encapsulation of the parasitic nematode Anguillicola crassus Kuwahara, Niimi & Hagaki is commonly observed in its native host, the Japanese eel (Anguilla japonica Temminck & Schlegel). Encapsulation has also been described in a novel host, the European eel (A. anguilla L.), and there is evidence that encapsulation frequency has increased since the introduction of A. crassus. We examined whether encapsulation
-
Phenotype, genotype and pathogenicity of Streptococcus agalactiae isolated from cultured tilapia (Oreochromis spp.) in Taiwan J. Fish Dis. (IF 2.318) Pub Date : 2020-12-03 Chiranan Sudpraseart; Pei‐Chi Wang; Shih‐Chu Chen
Tilapia (Oreochromis spp.) is globally used as an aquaculture fish species due to its high growth rate and disease resistance. However, it faces an increased risk of streptococcosis. Streptococcus agalactiae, also known as group B streptococcus (GBS), is the most important tilapia pathogen in Asia. Studies of Str. agalactiae infection in Taiwan are still unclear. Thus, this study aimed to explore the
-
The role of UhpA in regulating the virulence gene expression in Edwardsiella piscicida J. Fish Dis. (IF 2.318) Pub Date : 2020-11-27 Tingqi Ye; Cuimin Mu; Jiakang Chen; Guangchen Pan; Xuepeng Wang
Edwardsiella piscicida (E. piscicida) is an important fish pathogen. However, the mechanism of Glu6P transport regulatory protein UhpA how to affect the virulence gene expression in E. piscicida is still unclear. The results in this study showed that the metabolism‐related gene expression of cysteine synthase (orf 1134) and sulphate transporter (ychM) in the uhpA mutant strain ΔuhpA was 0.76‐fold and
-
Characterization and function study of a glutamyl endopeptidase homolog from Nocardia seriolae J. Fish Dis. (IF 2.318) Pub Date : 2020-11-27 Wenji Wang; Suying Hou; Guoquan Chen; Liqun Xia; Jianlin Chen; Zhiwen Wang; Yishan Lu
Glutamic endopeptidases (Glu), belonging to the class of serine proteases, are a subfamily of chymotrypsin‐like proteolytic enzymes, which are regarded as important virulence factors in bacteria. However, the roles of glutamic endopeptidases of Nocardia seriolae in pathogenic process still remain uncertain. Here, a glutamic endopeptidase homolog from N. seriolae (GluNS) was cloned and its function
-
Molecular cloning, characterization and expression analysis of major histocompatibility complex class I alpha gene of pufferfish (Takifugu obscurus) J. Fish Dis. (IF 2.318) Pub Date : 2020-11-21 Shengli Fu; Haisu Liu; Yu Huang; Junru Wang; Kun Qian; Mingmei Ding; Jianmin Ye
The major histocompatibility complex (MHC) genes play a key role in immune response in vertebrates. In this study, an MHC I alpha homolog gene (PfMHC Ⅰα) from pufferfish (Takifugu obscurus) was identified and its subcellular localization and expression patterns of PfMHC Ⅰα after challenge in vivo and in vitro were analysed. The open reading frame of PfMHC Ⅰα was 1,089 bp in length, encoding 362 aa
-
Amplicon sequencing reveals significantly increased Vibrio abundance and associated gene functions in vibriosis‐infected black tiger shrimp (Penaeus monodon) J. Fish Dis. (IF 2.318) Pub Date : 2020-11-18 Md Javed Foysal; Farhana Momtaz; A.Q.M. Robiul Kawser; Md Hazrat Ali; Topu Raihan; Muhammad A. B. Siddik; Md Mahbubur Rahman; Alfred Tay
Vibriosis caused by luminous Vibrio species is one of the biggest challenges to shrimp industry in Bangladesh. This study aimed to characterize whole microbial communities from Vibrio‐infected black tiger shrimp (Penaeus monodon) using 16S rRNA‐based amplicon sequencing. A total of 36 disease‐free and infected shrimp were collected from six different hatcheries in Bagerhat, Bangladesh. A final pool
-
Recombinant viral proteins delivered orally through inactivated bacterial cells induce protection in Macrobrachium rosenbergii (de Man) against White Tail Disease J. Fish Dis. (IF 2.318) Pub Date : 2020-11-18 Singaiah NaveenKumar; Praveen Rai; Indrani Karunasagar; Iddya Karunasagar
White tail disease (WTD) is a disease of Macrobrachium rosenbergii caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) with the potential to devastate the aquaculture industry. The present study aimed to explore the possible protection of M. rosenbergii against the disease by oral administration of bacterially expressed recombinant capsid proteins of MrNV and XSV. Juvenile
-
Cost‐effectiveness of longitudinal surveillance for Piscirickettsia salmonis using qPCR in Atlantic salmon farms (Salmo salar) in Chile J. Fish Dis. (IF 2.318) Pub Date : 2020-11-12 Marina K. V. C. Delphino; Fernando O. Mardones; Joaquin Neumann Heise; Alicia Gallardo; Daniel Jimenez; Andrea Peña; Marco Rozas‐Serri; Ian A. Gardner
Costs of diagnostic testing including sample collection, sampling frequency and sample size are an important consideration in the evaluation of the economic feasibility of alternative surveillance strategies for detection of infectious diseases in aquatic animals. In Chile, Piscirickettsia salmonis is the primary reason for antibiotic treatments in farmed Atlantic salmon. In 2012, a surveillance and
-
Silencing of prophenoloxidase (proPO) gene in freshwater prawn, Macrobrachium rosenbergii, makes them susceptible to white spot syndrome virus (WSSV) J. Fish Dis. (IF 2.318) Pub Date : 2020-11-10 S. Thamizhvanan; A. Nafeez Ahmed; D. Vinoth Kumar; S. Vimal; S. Abdul Majeed; G. Taju; Chris Hauton; A. S. Sahul Hameed
Prophenoloxidase (proPO) is very important to protect the invertebrates from microbial infections. Our previous studies revealed that proPO was up‐regulated in WSSV‐injected Macrobrachium rosenbergii and is responsible for protecting M. rosenbergii from WSSV. In order to prove this mechanism, an attempt was made in the present study to silence the proPO gene in freshwater prawn by injection of dsRNA‐proPO
-
Zebrafish (Danio rerio) larvae as a model for real‐time studies of propagating VHS virus infection, tissue tropism and neutrophil activity J. Fish Dis. (IF 2.318) Pub Date : 2020-11-10 Moonika Haahr Marana; Jacob Günther Schmidt; Stéphane Biacchesi; Niels Lorenzen; Louise von Gersdorff Jørgensen
Viral haemorrhagic septicaemia virus (VHSV) is a negative‐sense single‐stranded RNA virus that infects more than 140 different fish species. In this study, zebrafish larvae were employed as in vivo model organisms to investigate progression of disease, the correlation between propagation of the infection and irreversibility of disease, cell tropism and in situ neutrophil activity towards the VHSV‐infected
-
Case study development of a challenge test against Edwardsiella ictaluri in Mekong striped catfish (Pangasianodon hypophthalmus), for use in breeding: Estimates of the genetic correlation between susceptibilities in replicated tanks J. Fish Dis. (IF 2.318) Pub Date : 2020-11-09 Khoi Dinh Pham; Sang Van Nguyen; Jørgen Ødegård; Hans Magnus Gjøen; Gunnar Klemetsdal
Bacillary necrosis is a problematic disease in farming of Mekong striped catfish (Pangasianodon hypophthalmus). The pathogenic bacterium is Edwardsiella ictaluri, causing numerous white spots in swelled liver, kidney and spleen. An alternative to antibiotic treatment and vaccine is to select for improved genetic resistance to the disease that requires to establish a proper challenge test. Here, four
-
Method for cryopreservation of Paramoeba perurans J. Fish Dis. (IF 2.318) Pub Date : 2020-11-07 Christiane Tröße; Mats Kindt; Steffen Blindheim; Linda Andersen; Are Nylund
Paramoeba perurans causes amoebic gill disease (AGD), which is a major problem in aquaculture worldwide. The parasite can be cultured in vitro, but to this date, no method for long‐term storage of the clones exists. In this study, we describe a method for cryopreservation of Paramoeba perurans. The method was successfully employed on four out the five clones we tested. The thawing success rate, that
-
Rapid sequence modification in the highly polymorphic region (HPR) of the hemagglutinin gene of the infectious salmon anaemia virus (ISAV) suggests intra-segmental template switching recombination. J. Fish Dis. (IF 2.318) Pub Date : 2020-09-21 Matías Cárdenas,Claudia Galleguillos,Karina Acevedo,Catarina Ananias,Javiera Alarcón,Sofía Michelson,Jorge Toledo,Margarita Montoya,Claudio Meneses,Eduardo Castro-Nallar,Yesseny Vásquez-Martínez,Marcelo Cortez-San Martin
The ISAV has a genome composed of eight segments of (–)ssRNA, segment 6 codes for the hemagglutinin–esterase protein, and has the most variable region of the genome, the highly polymorphic region (HPR), which is unique among orthomyxoviruses. The HPR has been associated with virulence, infectivity and pathogenicity. The full length of the HPR is called HPR0 and the strain with this HPR is avirulent
-
Lethal concentration of Cymbopogon citratus (Poaceae) essential oil for Dolops discoidalis and Argulus sp. (Crustacea: Argulidae). J. Fish Dis. (IF 2.318) Pub Date : 2020-09-13 Elcimara Cardoso Pereira,Elzamara Castro Oliveira,Elen Monique Oliveira Sousa,Hugo Napoleão Pereira Silva,Lincoln Lima Corrêa,Rosa Helena Veras Mourão,Marcos Tavares-Dias,Lenise Vargas Flores Silva
This study investigated the acute toxicity (LC50‐24 hr) effects of the essential oil of Cymbopogon citratus for adult Argulus sp. and Dolops discoidalis, before and during oviposition. In vitro acute toxicity (LC50‐24 hr) was tested using 20, 40, 60, 80, 100, 120, 140 and 160 μg/L of C. citratus essential oil, and two control groups (one with cultivation tank water and one with cultivation tank water + alcohol)
-
Molecular and histopathological characterization of Photobacterium damselae in naturally and experimentally infected Nile tilapia (Oreochromis niloticus) J. Fish Dis. (IF 2.318) Pub Date : 2020-09-27 Mai A. M. El‐Son; Samia Elbahnaswy; Iman Ibrahim
Mass mortality has occurred among cultured Nile tilapia, Oreochromis niloticus, on fish farms in Manzala, Dakahlia province, Egypt, in the summer season, 2019. Moribund fish were reported with deep ulcers, septicaemic lesions and sampled for bacterial isolation. In this study, most isolates were subjected to bacteriological examination, antibiotic sensitivity test, 16S rRNA gene sequencing and histopathological
-
The effect of temperature on ability of Lepeophtheirus salmonis to infect and persist on Atlantic salmon. J. Fish Dis. (IF 2.318) Pub Date : 2020-09-03 Sussie Dalvin,Lars Are Hamre,Rasmus Skern-Mauritzen,Tone Vågseth,Lars Stien,Frode Oppedal,Samantha Bui
The salmon louse (Lepeophtheirus salmonis) is an ecologically and economically important parasite of salmonid fish. Temperature is a strong influencer of biological processes in salmon lice, with development rate increased at higher temperatures. The successful attachment of lice onto a host is also predicted to be influenced by temperature; however, the correlation of temperature with parasite survival
-
A C-type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus. J. Fish Dis. (IF 2.318) Pub Date : 2020-09-13 Xiao-Wen Zhang,Cong-Hui Yang,Hong-Quan Zhang,Xin-Tong Pan,Ze-Yu Jin,Hong-Wei Zhang,Xiao-Hua Xia
C‐type lectins are carbohydrate‐binding proteins that play important roles in immunity by serving as pattern recognition receptors. In the present study, a novel nattectin‐like C‐type lectin was obtained from the weather loach, Misgurnus anguillicaudatus, designated as MaCTL. MaCTL encodes a peptide with 165 amino acids, with a signal peptide and a single C‐type lectin domain (CTLD), containing a galactose‐specific
-
Role of major histocompatibility complex II antigen-presentation pathway genes in orange-spotted grouper infected with Cryptocaryon irritans. J. Fish Dis. (IF 2.318) Pub Date : 2020-09-13 Jun-Jin Deng,Shun Xu,Yan-Wei Li,Dong-Dong Xu,Ze-Quan Mo,Jia-Zhou Li,Xue-Ming Dan,Xiao-Chun Luo
Cryptocaryon irritans, a pathogen model for fish mucosal immunity, causes skin mucosal and systematic humoral immune response. Where and how MHC II antigen presentation occurs in fish infected with C. irritans remain unknown. In this study, the full‐length cDNA of the grouper cysteine protease CTSS was cloned. The expression distributions of six genes (CTSB, CTSL, CTSS, GILT, MHC IIA and MHC IIB) involved
-
Cathelicidins enhance protection of channel catfish, Ictalurus punctatus, and channel catfish ♀ × blue catfish, Ictalurus furcatus ♂ hybrid catfish against Edwarsiella ictaluri infection. J. Fish Dis. (IF 2.318) Pub Date : 2020-09-15 Rhoda Mae C Simora,Shangjia Li,Nermeen Y Abass,Jeffery S Terhune,Rex A Dunham
Cathelicidins are a class of antimicrobial peptides (AMPs) known to possess rapid and direct antimicrobial activities against a variety of microorganisms. Recently identified cathelicidins derived from alligator and sea snake were found to be more effective in inhibiting microbial growth than other AMPs previously characterized. The ability of these two cathelicidins along with the peptides, cecropin
-
Testicular leiomyoma and spermatogenic failure syndrome in a seabass from broodstock J. Fish Dis. (IF 2.318) Pub Date : 2020-09-24 Sabrina Natale; Fabiano Capparucci; Jessica Maria Abbate; Rosanna Panebianco; Roberto Puleio; Carmelo Iaria
Testicular leiomyomas and gonadal dysfunctions are not commonly reported in teleost and particularly in broodstock fish. In the present work, a testicular leiomyoma related to an unusual case of spermatogenic failure syndrome, in a broodstock seabass coming from an Italian aquaculture farm, is reported. At gross pathology, the circumscribed neoplasia showed several white nodular masses, originating
-
Infection of the Carpathian brook lamprey (Eudontomyzon danfordi Regan, 1911) with a dermocystid parasite in the Tisza River Basin, Hungary. J. Fish Dis. (IF 2.318) Pub Date : 2020-09-10 Boglárka Sellyei,Gábor Cech,Ádám Varga,Kálmán Molnár,Csaba Székely,Dóra Somogyi,Krisztián Nyeste,László Antal
The Carpathian brook lamprey (Eudontomyzon danfordi Regan, 1911) is an endemic protected species of Cephalaspidomorphi in the Carpathian Basin. No parasites have become known from these jawless vertebrates to date. Here, the authors describe an infection from a single specimen manifesting in protuberant skin cysts 7–10 mm in diameter, scattered on the body surface. Similar dermal infection was observed
-
Efficacy of a formalin-inactivated Lactococcus garvieae vaccine in farmed grey mullet (Mugil cephalus). J. Fish Dis. (IF 2.318) Pub Date : 2020-09-15 S Rao,O Byadgi,T Pulpipat,P-C Wang,S-C Chen
Lactococcosis [Lactococcus garvieae (LG)] is one of the most prevalent bacterial diseases affecting grey mullet (Mugil cephalus) aquaculture. Therefore, the present research evaluated the efficacy of formalin‐killed LG vaccine with an oil‐based adjuvant in grey mullet under laboratory and field trials. The laboratory evaluation for LG vaccine and its cross‐protection upon challenge in grey mullet found
-
Quantitative trait loci (QTL) associated with resistance of rainbow trout Oncorhynchus mykiss against the parasitic ciliate Ichthyophthirius multifiliis. J. Fish Dis. (IF 2.318) Pub Date : 2020-09-17 R Jaafar,J Ødegård,H Mathiessen,A M Karami,M H Marana,L von Gersdorff Jørgensen,S Zuo,T Nielsen,P W Kania,K Buchmann
The parasitic ciliate Ichthyophthirius multifiliis has a low host specificity eliciting white spot disease (WSD) in a wide range of freshwater fishes worldwide. The parasite multiplies rapidly whereby the infection may reach problematic levels in a host population within a few days. The parasite targets both wild and cultured fish but the huge economic impact of the protozoan is associated with mortality
-
Mucin histochemistry as a tool to assess rostral digestive tract health in a teleost model (Danio rerio). J. Fish Dis. (IF 2.318) Pub Date : 2020-09-03 Sanet H Kotzé,Ann Huysseune
The zebrafish (Danio rerio), a small agastric teleost fish, has become a popular species to model diseases of the gastrointestinal tract. Remarkably, its rostral digestive tract has been largely understudied. To allow for accurate reporting and to improve the translational potential of this research model, the present study first proposes terminology for the different regions of the rostral digestive
-
Development of a real‐time recombinase polymerase amplification assay for rapid detection of Aeromonas hydrophila J. Fish Dis. (IF 2.318) Pub Date : 2020-11-05 Yang Qu; Qing Wang; Yingying Li; Yingying Wang; Jiyuan Yin; Yan Ren; Chun Liu; Xiaofang Liu; Yahui Wang; Weiwei Zeng
Aeromonas hydrophila is ubiquitous in the aquaculture industry and a constant cause of severe disease and economic losses. The early diagnosis of these infections is crucial for disease surveillance and prevention. We developed a real‐time recombinase polymerase amplification (real‐time RPA) assay for detection of A. hydrophila using the haemolysin gene. The assay was performed at 37°C for 20 min and
-
Hydrogen peroxide treatment and its impacts on Lepeophtheirus salmonis originating from the Bay of Fundy, Canada J. Fish Dis. (IF 2.318) Pub Date : 2020-11-04 Carter R. VanIderstine; Jordan D. Poley; Shona K. Whyte; Sara L. Purcell; Mark D. Fast
Hydrogen peroxide (H2O2) is used to treat sea lice infections of farmed salmonids in the Atlantic and Pacific Oceans and issues with resistance to this treatment, and others are a major threat to the sustainability of the industry. The objectives of this study were to determine how H2O2 exposure affects survival and antioxidant‐related gene expression in salmon lice (Lepeophtheirus salmonis) collected
-
Mycoplasma infections in freshwater carnivorous fishes in Hungary J. Fish Dis. (IF 2.318) Pub Date : 2020-10-31 Boglárka Sellyei; Zsuzsanna Varga; Gábor Cech; Ádám Varga; Csaba Székely
The abundance of mycoplasma‐like microorganisms seems to be common in the intestinal tract of fish, but their occurrence in other organs can be considered pathological. Isolation of piscine mycoplasmas is essential to prove their virulence. In this study, a total of 100 specimens of nine freshwater fish species collected from natural waters and fish farms were analysed for the prevalence of mycoplasmas
-
Effects of Greek juniper (Juniperus excelsa) extract on immune responses and disease resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss) J. Fish Dis. (IF 2.318) Pub Date : 2020-10-31 Soner Bilen; Selcuk Ispir; Osman Nezih Kenanoglu; Yiğit Taştan; Kerim Güney; Ertugrul Terzi
This study investigated the effects of Greek juniper extract on immune responses of rainbow trout. In this experiment, 4 doses [0 (Control), 1 (J1), 4 (J4) and 8 (J8) mg/kg] of the extract were administered orally using an oral gavage twice a day for 14 days. Immune responses were measured on 7th and 14th days. On 14th day, Yersinia ruckeri was injected intraperitoneally to all fish of all groups.
-
Effect of handling and crowding on the susceptibility of Atlantic salmon (Salmo salar L.) to Lepeophtheirus salmonis (Krøyer) copepodids J. Fish Dis. (IF 2.318) Pub Date : 2020-10-28 Cyril Delfosse; Patrick Pageat; Céline Lafont‐Lecuelle; Pietro Asproni; Camille Chabaud; Alessandro Cozzi; Cécile Bienboire‐Frosini
Lepeophtheirus salmonis is an ectoparasite causing economic concerns in Atlantic salmon farming. Salmon lice infestation management methods can be stressful and impact fish welfare. This work investigated the stress effect on the attachment of L. salmonis copepodids to Atlantic salmon through two approaches: (a) handling by netting and air exposure (acute stress), and (b) crowding with restricted surface
-
Detection of scale drop disease virus from non‐destructive samples and ectoparasites of Asian sea bass, Lates calcarifer J. Fish Dis. (IF 2.318) Pub Date : 2020-10-28 Onanong Charoenwai; Saengchan Senapin; Ha Thanh Dong; Molruedee Sonthi
Non‐destructive sampling methods offer practical advantages to detection and monitoring of viral pathogens in economically important farmed fish and broodstock. Here, we investigated whether blood, mucus and fin can be used as non‐lethal sample sources for detection of scale drop disease virus (SDDV) in farmed Asian sea bass, Lates calcarifer. Detection of SDDV was performed in parallel from three
-
A multi‐biomarker study on Atlantic salmon (Salmo salar L.) affected by the emerging Red Skin Disease in the Baltic Sea J. Fish Dis. (IF 2.318) Pub Date : 2020-10-26 Fabian G. Weichert; Charlotte Axén; Lars Förlin; Pedro A. Inostroza; Ulrike Kammann; Annikki Welling; Joachim Sturve; Noomi Asker
For half a decade, the Atlantic salmon in the Baltic Sea has been facing severe health issues. Clinical signs like haemorrhage, erosions and ulcerative/necrotic skin conditions in returning adults have been reported from different Swedish rivers. These primary disease signs precede a secondary, terminal fungal infection. As initial investigations of the disease did not provide conclusive answers regarding
-
Genome‐wide analysis of Toll‐like receptors in zebrafish and the effect of rearing temperature on the receptors in response to stimulated pathogen infection J. Fish Dis. (IF 2.318) Pub Date : 2020-10-26 Hong Chen; Yue Liang; Yawen Han; Tengfei Liu; Shulin Chen
Water temperature has a major influence on the host innate immune defence and the infectivity of pathogens in ectothermic teleosts. Toll‐like receptors (TLRs) are the first and well‐characterized innate immune receptors that are conserved in vertebrates. However, little is known about the effect of temperature variation on TLRs in fish species. In this study, we used adult zebrafish as a research model
-
Lipid metabolism disorders contribute to the pathogenesis of Hepatospora eriocheir in the crab Eriocheir sinensis J. Fish Dis. (IF 2.318) Pub Date : 2020-10-26 Zhengfeng Ding