-
Enterolith with a bonefish spine nidus in a Brazilian cownose ray Rhinoptera brasiliensis (Müller & Henle) (Myliobatiformes: Rhinopteridae) J. Fish Dis. (IF 2.5) Pub Date : 2024-03-16 L. O. Camilo, B. L. Maganhe, H. Gallo Neto, E. G. Sanches
An adult female Brazilian cownose ray (Rhinoptera brasiliensis), housed in Ubatuba Aquarium died after loss of appetite period. During necropsy, an enterolith was discovered partially obstructing the intestinal lumen. Examination of the enterolith revealed a bonefish spine nidus. Enterolithiasis has been linked to multiple factors including diet, genetics, alkaline intestinal environments and, as in
-
Shewanella xiamenensis‐associated ulcerative dermatitis in koi carp (Cyprinus rubrofuscus) J. Fish Dis. (IF 2.5) Pub Date : 2024-03-16 Andrew F. Rich, Mark Naguib
Ulcerative dermatitis (UD) is common in ornamental fish collections and is typically associated with a wide range of bacterial aetiologies. Clinical reports describing Shewanella xiamenensis‐associated UD are limited, however, despite growing attention to pathogenic Shewanella species in fish. Two out of 95 koi carp with UD were presented for clinical assessment by a commercial collection (n = 3000
-
Clinical symptoms and histopathological changes in coho salmon affected by the erythrocytic inclusion body syndrome (EIBS) are caused by the infection of piscine orthoreovirus 2 (PRV‐2) J. Fish Dis. (IF 2.5) Pub Date : 2024-03-14 Tomokazu Takano, Satoshi Miwa, Tomomasa Matsuyama, Ikunari Kiryu, Miho Honjo, Takamitsu Sakai, Yuta Matsuura, Masatoshi Yamasaki, Akira Kumagai, Chihaya Nakayasu
The relationship of histopathological changes and the infection of Piscine orthoreovirus 2 (PRV‐2) was investigated in coho salmon that were suffering from the erythrocytic inclusion body syndrome (EIBS). Immunohistochemical observations revealed abundant σ1 protein of PRV‐2 in the spongy layer of the ventricle of the heart, where severe myocarditis was observed. In the spleen, the virus protein was
-
Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine J. Fish Dis. (IF 2.5) Pub Date : 2024-03-14 Nur Shidaa Mohd Ali, Mohamad Syazwan Ngalimat, Mohd Zamri Saad, Mohammad Noor Amal Azmai, Annas Salleh, Zarirah Zulperi, Ina Salwany Md Yasin
Streptococcosis and aeromoniasis are the main obstacles to sustainable tilapia production. Vaccination offered an effective method to control microbial infections. Previously, a feed‐based bivalent vaccine (FBBV) containing killed whole organisms of Streptococcus agalactiae and Aeromonas hydrophila mixed with 10% palm oil was successfully developed, which provided good protection against streptococcosis
-
Novel Aquareovirus isolated from channel catfish (Ictalurus punctatus) used in mussel restoration efforts in Wisconsin J. Fish Dis. (IF 2.5) Pub Date : 2024-03-11 Eric Leis, Ryan Katona, Sara Dziki, Rebekah McCann, Isaac Standish, Eryn Opgenorth, Audrey Dikkeboom, Ailam Lim, Charlotte E. Ford, Tony L. Goldberg
Channel catfish (Ictalurus punctatus) are a food fish extensively reared in aquaculture facilities throughout the world and are also among the most abundant wild catfish species in North America, making them a popular target of anglers. Furthermore, channel catfish are important members of aquatic ecosystems; for example, they serve as a glochidial host for the endangered winged mapleleaf mussel (Quadrula
-
-
Diagnosis, isolation and description of a novel amnoonvirus recovered from diseased fancy guppies, Poecilia reticulata J. Fish Dis. (IF 2.5) Pub Date : 2024-03-05 Esteban Soto, Benjamin R. LaFrentz, Susan Yun, Dorothea Megarani, Eileen Henderson, Chutchai Piewbang, Amber E. Johnston, Somporn Techangamsuwan, Terry Fei Fan Ng, Janet Warg, Win Surachetpong, Kuttichantran Subramaniam
The guppy, Poecilia reticulata, is one of the most common cultured ornamental fish species, and a popular pet fish highly desired by hobbyists worldwide due to its availability of many brilliantly coloured fish of many varieties. The susceptibility of guppies to diseases presents a remarkable concern for both breeders and hobbyists. In this study, we report the emergence of disease in fancy guppies
-
In vitro effects of phytogenic feed additive on Piscirickettsia salmonis growth and biofilm formation J. Fish Dis. (IF 2.5) Pub Date : 2024-02-29 Natacha Santibáñez, Matías Vega, Tatiana Pérez, Ricardo Enriquez, Carla Estefanía Escalona, Cristian Oliver, Alex Romero
Piscirickettsiosis is the main cause of mortality in salmonids of commercial importance in Chile, which is caused by Piscirickettsia salmonis, a Gram‐negative, γ‐proteobacteria that can produce biofilm as one of its virulence factors. The Chilean salmon industry uses large amounts of antibiotics to control piscirickettsiosis outbreaks, which has raised concern about its environmental impact and the
-
First report of Metschnikowia bicuspidata infection in the oriental river prawn (Macrobrachium nipponense, de Haan) in China J. Fish Dis. (IF 2.5) Pub Date : 2024-02-29 Yingyue Yu, Songyue You, Chengcheng Feng, Xiaodong Li, Yuenan Xing, Qingbiao Hu, Jie Bao, Hongbo Jiang
During breeding, some oriental river prawns (Macrobrachium nipponense, de Haan), an important aquaculture species in China, exhibit yellowish‐brown body colouration, reduced appetite, and vitality. Diseased prawns revealed characteristic emulsifying disease signs, including whitened musculature, hepatopancreatic tissues, milky haemolymph, and non‐coagulation. The present study investigated the causative
-
Potential to grow carp oedema virus (genogroup I) in monolayers of carp‐derived primary cells with further implication in cell analysis J. Fish Dis. (IF 2.5) Pub Date : 2024-02-29 Ehdaa Eltayeb Eltigani Abdelsalam, Zuzana Bláhová, Ali Asghar Baloch, Veronika Piačková
Carp oedema virus (CEV) has distinct molecularly identified genogroups of viral mutations, denoted as I, IIa, and IIb. Failure to propagate CEV in vitro limits studies towards understanding its interactions with host cells. Here, virus isolates belonging to genogroup I collected during natural outbreaks in the Czech Republic were employed for routine CEV cultivation in monolayers of carp‐derived primary
-
Serine protease inhibitor 3 (Serpin3) from Penaeus vannamei selectively interacts with Vibrio parahaemolyticusPirAvp J. Fish Dis. (IF 2.5) Pub Date : 2024-02-26 Thanh‐Nguyen Le, Thuan‐Thien Dinh, Thuy‐Dung Mai‐Hoang, Ebrahim Razzazi‐Fazeli, Hieu Tran‐Van
Acute Hepatopancreatic Necrosis Disease (AHPND) represents a significant challenge in the field of shrimp aquaculture. This disease is primarily caused by Vibrio parahaemolyticus strains harbouring the pVA1 plasmid encoding the PirAvp and PirBvp toxins. To combat this epidemic and mitigate its devastating consequences, it is crucial to identify and characterize the receptors responsible for the binding
-
Identification of new amoebae strains in rainbow trout (Oncorhynchus mykiss, Walbaum) farms affected by nodular gill disease (NGD) in Northeastern Italy J. Fish Dis. (IF 2.5) Pub Date : 2024-02-24 Ginevra Brocca, Alessandro Truant, Hana Peckova, Martina Lisnerová, Alberto Perolo, Marialetizia Fioravanti, Ivan Fiala, Gianfranco Gabai, Francesco Quaglio, Andrea Gustinelli
Nodular gill disease (NGD) is an emerging condition associated with amoeba trophozoites in freshwater salmonid farms. However, unambiguous identification of the pathogens still must be achieved. This study aimed to identify the amoeba species involved in periodic NGD outbreaks in two rainbow trout (Oncorhynchus mykiss) farms in Northeastern Italy. During four episodes (February–April 2023), 88 fish
-
Unveiling the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing and virulence gene expression and enhancing immunity J. Fish Dis. (IF 2.5) Pub Date : 2024-02-19 Widanarni Widanarni, Muhamad Gustilatov, Julie Ekasari, Pande Gde Sasmita Julyantoro, Diana Elizabeth Waturangi, Sukenda Sukenda
This study aimed to evaluate and unveil the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing (QS) and virulence gene expression and enhancing shrimp's immunity. The shrimp with an average body weight of 0.50 ± 0.09 g were reared in containers with a volume of 2.5 L, 21 units, and a density of 20 shrimp L−1. The shrimp were cultured
-
Comparative phenotype and transcriptome analysis revealed the role of ferric uptake regulator (Fur) in the virulence of Vibrio harveyi isolated from diseased American eel (Anguilla rostrata) J. Fish Dis. (IF 2.5) Pub Date : 2024-02-19 Qijuan Wan, Shaowei Zhai, Minxia Chen, Ming Xu, Songlin Guo
Vibrio harveyi is commonly found in salt and brackish water and is recognized as a serious bacterial pathogen in aquaculture worldwide. In this study, we cloned the ferric uptake regulator (fur) gene from V. harveyi wild‐type strain HA_1, which was isolated from diseased American eels (Anguilla rostrata) and has a length of 450 bp, encoding 149 amino acids. Then, a mutant strain, HA_1‐Δfur, was constructed
-
Rapid and sensitive detection of large yellow croaker iridovirus by real-time RPA and RPA-LFD J. Fish Dis. (IF 2.5) Pub Date : 2024-02-13 Xiaoru Liu, Yong Cao, Jiayin Wang, Suyuheng Cao, Liqun Lu, Yousheng Jiang
Large yellow croaker (Larimichthys crocea) is a vital marine-cultured species in China. Large yellow croaker iridovirus (LYCIV) can cause a high mortality rate in L. crocea. Rapid and convenient detection of LYCIV is an urgent demand for diagnosis. In this study, rapid and simple recombinase polymerase amplification (RPA), real-time RPA and RPA combined with lateral flow dipstick (RPA-LFD) methods
-
Investigation of extragonadal teratomas in two Poecilia wingei J. Fish Dis. (IF 2.5) Pub Date : 2024-01-30 Denis V. Prazdnikov, Ekaterina A. Kondakova
Teratoma is a rare tumour in fish consisting of tissues from more than one germ layer, that may be located in either the gonads or extragonadal sites. Teratomas in many fish species remain poorly understood. In this work, we performed the first histological examinations of extragonadal teratomas in Poecilia wingei and also examined the influence of a large teratoma on male sexual activity. The studied
-
Establishment of a real-time PCR for the detection of decapod iridescent virus 1 (DIV1) J. Fish Dis. (IF 2.5) Pub Date : 2024-02-01 Xiao-Meng Guo, Jing-Yi Xing, Anqi Li, Liang Qiu, Qing-Li Zhang, Jie Huang
CONFLICT OF INTEREST STATEMENT All authors declare that there is no conflict of interest or financial interest.
-
Phytochemical analysis and antibacterial activity of palm waste extract against Vibrio harveyi and Vibrio parahaemolyticus J. Fish Dis. (IF 2.5) Pub Date : 2024-02-01 Nasmia Nasmia, Novalina Serdiati, Akbar Marzuki Tahya, Muhammad Safir
Vibrio harveyi and Vibrio parahaemolyticus are species of the Vibrio genus that often cause disease and mass mortality in crustaceans. If not handled quickly and appropriately, these diseases can cause considerable losses to farmers. Therefore, it is necessary to find a solution with safe and environmentally friendly disease prevention technology using natural ingredients, among others from plants
-
Development and characterization of brain cell line from Trachinotus blochii and its application in virological and gene expression studies J. Fish Dis. (IF 2.5) Pub Date : 2024-01-29 A. Nafeez Ahmed, S. Mithra, S. Suryakodi, G. Taju, M. J. Abdul Wazith, K. Kanimozhi, V. Rajkumar, A. Badhusha, S. Abdul Majeed, A. S. Sahul Hameed
A permanent cell line, SPB (Snubnose pompano brain) was established from Trachinotus blochii by the explant culture method. It has been sub-cultured more than 75 passages and showed optimal growth at 28°C using L-15 medium supplemented with 15% to 20% FBS. The SPB cells were cryopreserved at different passage levels for various applications. SPB cells were composed of fibroblastic and epithelial-like
-
Pathogenic bacteria prevalence in cultured Nile tilapia in Southwest Mexico: A real-time PCR analysis J. Fish Dis. (IF 2.5) Pub Date : 2024-01-25 Sonia A. Soto-Rodriguez, Francis I. Marrujo Lopez, Karla G. Aguilar-Rendon, Rafael Hernández Guzmán
The present study investigates molecular-based PCR techniques to estimate the prevalence of fish pathogens in southwest Mexico where recurrent mortality in the tilapia cultures has been observed. Sample of internal organs and lesions of Nile tilapia were taken and analysed in 2018, 2019, 2020 and 2022 to detect bacterial pathogens using PCR. No samples were taken in 2021 due to the COVID-19 pandemic
-
Dimethomorph activity and its effect on morphology in different oomycete species of economic and veterinary interest J. Fish Dis. (IF 2.5) Pub Date : 2024-01-24 Irene Maja Nanni, Perla Tedesco, David Baldo, Roberta Galuppi, Marina Collina
CONFLICT OF INTEREST STATEMENT The authors declare no conflict of interest.
-
First report and pathogenicity of Vibrio campbellii (VCAHPND) isolated in South Korea J. Fish Dis. (IF 2.5) Pub Date : 2024-01-24 Hee-Jae Choi, Da-Yeon Choi, Ji-Hoon Lee, Jun-Hwan Kim, Yue Jai Kang
CONFLICT OF INTEREST STATEMENT The authors declare no conflict of interest to declare.
-
Bacterial abscesses in whitespotted bamboo sharks (Chiloscyllium plagiosum) in managed care J. Fish Dis. (IF 2.5) Pub Date : 2024-01-19 Alexandra K. Mason, Lance Adams, Michael M. Garner
Sixteen whitespotted bamboo sharks (Chiloscyllium plagiosum) with histologically similar bacterial abscesses were submitted to Northwest ZooPath from nine zoo and aquarium institutions over a 17-year period. These abscesses were characterized by inflammatory cell infiltrates and necrosis with intralesional small, Gram-positive, acid-fast negative, cocci bacteria. The clinical presentation, histologic
-
Non-lethal detection of Eubothrium crassum (Cestoda) in farmed Atlantic salmon, Salmo salar, using anal swabs and real-time PCR J. Fish Dis. (IF 2.5) Pub Date : 2024-01-18 Haakon Hansen, Bjørn Spilsberg, Sigmund Sevatdal, Trine Sakariassen, Christoph Hahn, Saima Nasrin Mohammad, Egil Karlsbakk
Detection of intestinal parasites in fish typically requires autopsy, resulting in the sacrifice of the fish. Here, we describe a non-lethal method for detecting the tapeworm Eubothrium crassum in fish using anal swabs and real-time PCR detection. Two assays were developed to detect cytochrome oxidase I (COI) mitochondrial DNA and 18S ribosomal DNA sequences of E. crassum, respectively. The assays
-
Tail Fan Necrosis syndrome in decapod crustaceans: A review J. Fish Dis. (IF 2.5) Pub Date : 2024-01-16 John Brian Jones, Cara L. Brosnahan, Anjali Pande
Lobsters and crayfish in Australasia can develop a condition known as Tail Fan Necrosis (TFN syndrome). Many attempts have been made to find a primary pathogen or link the syndrome to commercial activities, but a solution remains elusive. TFN syndrome is a ‘wicked problem’, a problem difficult or impossible to solve because of incomplete and contradictory information forming a matrix of potential outcomes
-
Synergistic interaction of nanoparticles and probiotic delivery: A review J. Fish Dis. (IF 2.5) Pub Date : 2024-01-16 Srirengaraj Vijayaram, Hary Razafindralambo, Yun Zhang Sun, Giuseppe Piccione, Cristiana Roberta Multisanti, Caterina Faggio
Nanotechnology is an expanding and new technology that prompts production with nanoparticle-based (1–100 nm) organic and inorganic materials. Such a tool has an imperative function in different sectors like bioengineering, pharmaceuticals, electronics, energy, nuclear energy, and fuel, and its applications are helpful for human, animal, plant, and environmental health. In exacting, the nanoparticles
-
Establishment of a gill cell line from yellowfin seabream (Acanthopagrus latus) for studying Amyloodinium ocellatum infection of fish J. Fish Dis. (IF 2.5) Pub Date : 2024-01-13 Zhicheng Li, Jingyu Zhuang, Jiaming Chen, Jizhen Cao, Qing Han, Zhi Luo, Baotun Wang, Hebing Wang, Anxing Li
Amyloodinium ocellatum is among the most devastating protozoan parasites, causing huge economic losses in the mariculture industry. However, the pathogenesis of amyloodiniosis remains unknown, hindering the development of targeted anti-parasitic drugs. The A. ocellatum in vitro model is an indispensable tool for investigating the pathogenic mechanism of amyloodiniosis at the cellular and molecular
-
Coinfection of channel catfish (Ictalurus punctatus) with virulent Aeromonas hydrophila and Flavobacterium covae exacerbates mortality J. Fish Dis. (IF 2.5) Pub Date : 2024-01-12 Allison L. Wise, Benjamin R. LaFrentz, Anita M. Kelly, Mark R. Liles, Matt J. Griffin, Benjamin H. Beck, Timothy J. Bruce
Flavobacterium covae and virulent Aeromonas hydrophila are prevalent bacterial pathogens within the US catfish industry that can cause high mortality in production ponds. An assessment of in vivo bacterial coinfection with virulent A. hydrophila (ML09-119) and F. covae (ALG-00-530) was conducted in juvenile channel catfish (Ictalurus punctatus). Catfish were divided into seven treatments: (1) mock
-
Isolation, identification and characterization of Aeromonas jandaei from diseased Chinese soft-shell turtles J. Fish Dis. (IF 2.5) Pub Date : 2024-01-13 Mengmeng Chen, Mingyang Xue, Jingtao Chen, Zidong Xiao, Xiaowei Hu, Chunjie Zhang, Nan Jiang, Yuding Fan, Yan Meng, Yong Zhou
Aeromonas jandaei is a gram-negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft-shell turtle (Pelodiscus sinensis), then named strain JDP-FX. This isolate was identified as A. jandaei after analysis of morphological, physiological and biochemical
-
A novel defensin-like peptide C-13326 possesses protective effect against multidrug-resistant Aeromonas schubertii in hybrid snakehead (Channa maculate ♀ × Channa argus ♂) J. Fish Dis. (IF 2.5) Pub Date : 2024-01-10 Huanrong He, Xinzhi Huang, Caiyi Wen, Chun Liu, Biao Jiang, Yanhua Huang, Youlu Su, Wei Li
The purpose of this study was to investigate whether a defensin-like antimicrobial peptide (C-13326 peptide) identified in Hermetia illucens could possess protective effect against multidrug-resistant Aeromonas schubertii in hybrid snakehead (Channa maculate ♀ × Channa argus ♂). The cDNA of C-13326 peptide comprised 243 nucleotides encoding 80 amino acids, with six conserved cysteine residues and the
-
Mixed Eimeria and Cryptosporidium infection and its effects on pathology and clinical outcomes in juvenile Asian seabass (Lates calcarifer) cultured in Thailand J. Fish Dis. (IF 2.5) Pub Date : 2024-01-07 Watcharapol Suyapoh, Narissara Keawchana, Peerapon Sornying, Sirikachorn Tangkawattana, Pokphon Khirilak, Sasibha Jantrakajorn
Coccidiosis is an important disease in juvenile fish because of severe intestinal injury during infection. We first reported the mixed infection of intestinal coccidia and its association with health status and pathological findings in juvenile Asian seabass (Lates calcarifer) cultured in Thailand. Two groups of Asian seabass, 60-day fish and 90-day fish, were sampled to investigate prevalence and
-
Natural product honokiol exhibits antiviral effects against Micropterus salmoides rhabdovirus (MSRV) both in vitro and in vivo J. Fish Dis. (IF 2.5) Pub Date : 2024-01-08 Fei Yang, Bin Yang, Kaige Song, Yingjie Jin, Gaoxue Wang, Pengfei Li, Qing Yu, Fei Ling
Micropterus salmoides rhabdovirus (MSRV) is a formidable pathogen, presenting a grave menace to juvenile largemouth bass. This viral infection frequently leads to epidemic outbreaks, resulting in substantial economic losses within the aquaculture industry. Unfortunately, at present, there are no commercially available vaccines or pharmaceutical treatments to combat this threat. In order to address
-
Influence of probiotic and prebiotic supplementation on intestinal microbiota and resistance to Edwardsiella ictaluri infection in channel catfish (Ictalurus punctatus) following florfenicol administration J. Fish Dis. (IF 2.5) Pub Date : 2023-12-28 Caitlin E. Older, Matt J. Griffin, Bradley M. Richardson, Geoffrey C. Waldbieser, J. Grant Reifers, Penelope M. Goodman, Cynthia Ware, Delbert M. Gatlin, David J. Wise, Fernando Y. Yamamoto
Enteric septicemia of catfish (ESC), caused by the gram-negative enteric bacteria Edwardsiella ictaluri, is a significant threat to catfish aquaculture in the southeastern United States. Antibiotic intervention can reduce mortality; however, antibiotic use results in an imbalance, or dysbiosis, of the gut microbiota, which may increase susceptibility of otherwise healthy fish to enteric infections
-
Study of the frequencies of erythrocyte abnormalities as in situ biomarkers of genotoxic risk of chemicals in special fish stock in water supply reservoirs J. Fish Dis. (IF 2.5) Pub Date : 2023-12-27 Jiří Řehulka, Jaroslav Bradík
In three water-supply reservoirs in the catchment area of the Odra River (Czech Republic), a special fish stock was monitored for control of health to estimate the mutagenic effect of chemicals. The results contribute to obtaining initial information about the morphology of erythrocyte abnormalities classified in 21 categories in 16 fish species in reservoirs with abundant salmonids (the Morávka Reservoir)
-
Molecular quantification of parasitic sea louse larvae depends on species and life stage J. Fish Dis. (IF 2.5) Pub Date : 2023-12-25 Nathan E. Mertz, Rachel A. Paterson, Bengt Finstad, Hege Brandsegg, Ida Pernille Øystese Andersskog, Frode Fossøy
Sea lice cause substantial economic and environmental harm to Norway's aquaculture industry and wild salmonid populations. Rapid, accurate quantification of lice larval densities in coastal waters remains the greatest bottleneck for providing empirical data on infestation risk within wild salmon habitats and aquaculture production regions. We evaluated the capability of droplet digital PCR (ddPCR)
-
Basibranchial structure affecting cardiac morphology in Atlantic salmon (Salmo salar L.) J. Fish Dis. (IF 2.5) Pub Date : 2023-12-25 Trygve T. Poppe, Anne Katrine Reed, Helene Wisløff
CONFLICT OF INTEREST STATEMENT The authors declare no conflicts of interest.
-
Effect of temperature on the protective efficacy of a live attenuated vaccine against herpesviral haematopoietic necrosis in goldfish J. Fish Dis. (IF 2.5) Pub Date : 2023-12-19 Hiroaki Saito, Shungo Minami, Manami Yuguchi, Aiko Shitara, Hidehiro Kondo, Goshi Kato, Motohiko Sano
The live attenuated vaccine P7-P8 strain against herpesviral haematopoietic necrosis, which is caused by cyprinid herpesvirus 2 (CyHV-2), exhibits high protective efficacy in goldfish at 25°C, the predominant temperature for this disease; however, the effect of water temperature during the vaccination period on efficacy has not been determined. In this study, an in vitro experiment revealed that the
-
Development and application of a recombinase-aided amplification combined with a lateral flow dipstick assay for rapid visual detection of anguillid herpesvirus 1 J. Fish Dis. (IF 2.5) Pub Date : 2023-12-19 Xi Chen, Hua Chen, Jun-Qing Ge
Eel (Anguilla sp.) is an important freshwater-cultured species with high economic value in China. Anguillid herpesvirus 1 (AngHV-1) has been proven to be the pathogen of “mucus sloughing and haemorrhagic septicaemia disease” in eels, resulting in significant mortality and substantial losses to the eel industry. Current diagnostic methods for detecting AngHV-1 are limited to laboratory-based tests,
-
Diversity of fungus-like stramenopilous organisms (Oomycota) in Lithuanian freshwater aquaculture: Morphological and molecular analysis, risk to fish health J. Fish Dis. (IF 2.5) Pub Date : 2023-12-12 Svetlana Markovskaja, Reda Iršėnaitė, Audrius Kačergius, Gintarė Sauliutė, Milda Stankevičiūtė
The present work is the first comprehensive study of fungus-like stramenopilous organisms (Oomycota) diversity in Lithuanian fish farms aimed at proper identification of saprolegniasis pathogens, which is important for water quality control, monitoring infection levels and choosing more effective treatments for this disease in aquaculture. Pathogenic to fish, Saprolegnia and other potentially pathogenic
-
Confirmatory test of active IHHNV infection in shrimp by immunohistochemistry and IHHNV-LongAmp PCR J. Fish Dis. (IF 2.5) Pub Date : 2023-12-10 Supapong Imsonpang, Arnon Pudgerd, Charoonroj Chotwiwatthanakun, Jiraporn Srisala, Piyachat Sanguanrut, Chanadda Kasamechotchung, Kallaya Sritunyalucksana, Suparat Taengchaiyaphum, Rapeepun Vanichviriyakit
The presence of endogenous viral elements (EVE) in the penaeid shrimp genome has been recently reported and suggested to be involved in the host recognition of viral invaders. Our previous report of a search for EVE of infectious hypodermal and haematopoietic necrosis virus (IHHNV-EVE) in the Thai Penaeus monodon whole genome sequence project (GenBank accession no. JABERT000000000) confirmed the presence
-
Establishment of a TaqMan probe-based qPCR assay for detecting microsporidia Enterospora epinepheli in grouper J. Fish Dis. (IF 2.5) Pub Date : 2023-12-07 Quan-lin Liu, Yuan Wang, Jie Chen, Guo-qing Pan, Yan-Feng Yue, Ze-Yang Zhou, Wen-hong Fang
Enterospora epinepheli is an intranuclear microsporidian parasite causing serious emaciative disease in hatchery-bred juvenile groupers (Epinephelus spp.). Rapid and sensitive detection is urgently needed as its chronic infection tends to cause emaciation as well as white faeces syndrome and results in fry mortality. This study established a TaqMan probe-based real-time quantitative PCR assays targeting
-
Identification of a potential antigen stimulating immune response against Vibrio parahaemolyticus infection in hybrid tilapia (Oreochromis aureus♂ × Oreochromis niloticus♀) J. Fish Dis. (IF 2.5) Pub Date : 2023-12-08 Qingsong Zeng, Yunxiao Sun, Peifang Lai, Mingqin Huang, Ximing Peng, Junjie Huang, Qintao Chen, Yanxu Chen, Huaqian Wang
Vibrio parahaemolyticus (V. parahaemolyticus) is a major pathogen that causes substantial losses in the marine fishery. With the emergence of antibiotic resistance, vaccines have become the most effective approach against V. parahaemolyticus infection. Adhesion factors on the cell surface are pivotal in the colonization and pathogenesis of V. parahaemolyticus within the host, highlighting their potential
-
Disruption of a kasB homolog gene (kasB) causes attenuation of cell invasion and virulence of Nocardia seriolae J. Fish Dis. (IF 2.5) Pub Date : 2023-12-06 Su-Ming Zhou, Feng-Ling Shu, Hao Liu, Dong Qian, Jia-Song Xie, Ya-Jun Wang, Fei Yin
Nocardia seriolae is the primary aetiological agent of nocardiosis in fish, which causes mass mortality in freshwater and marine fish. β-ketoacyl-ACP synthase (KAS) is one of the essential enzymes in the synthesis of mycolic acids (MASs) in Mycobacterium spp. and has been chosen as the target for therapeutic intervention in mycobacterial diseases. In the present study, a kasB homologue gene (kasB)
-
Magnetic resonance imaging shows spinal curvature in Chinook salmon (Oncorhynchus tshawytscha) is associated with chronic inflammation of peri-vertebral soft tissues J. Fish Dis. (IF 2.5) Pub Date : 2023-12-06 Bailey A. Lovett, Elwyn C. Firth, Matthew R. Perrott, John S. Munday, Beau P. Pontre, Anna-Maria P. Lydon, Jane E. Symonds, Mark A. Preece, Neill A. Herbert
Chinook salmon (Oncorhynchus tshawytscha) farmed in New Zealand are known to develop abnormal spinal curvature late in seawater production. Its cause is presently unknown, but there is evidence to suggest a neuromuscular pathology. Using magnetic resonance imaging (MRI), we evaluated the relationship between soft tissue pathology and spinal curvature in farmed Chinook salmon. Regions of interest (ROIs)
-
Iron supplementation in the diets of hybrid catfish (Ictalurus punctatus × I. furcatus) juveniles affected haematocrit levels and potentially decreased disease resistance to Edwardsiella ictaluri J. Fish Dis. (IF 2.5) Pub Date : 2023-12-01 Fernando Y. Yamamoto, Matt J. Griffin, Bradley M. Richardson, Justin M. Stilwell, Nicholas Romano, Penelope M. Goodman, J. Grant Reifers, David J. Wise
To prevent catfish idiopathic anaemia, diets fortified with iron have been adopted as a regular practice on commercial catfish farms to promote erythropoiesis. However, the effects of prolonged exposure of excess dietary iron on production performance and disease resistance for hybrid catfish (Ictalurus punctatus × I. furcatus) remains unknown. Four experimental diets were supplemented with ferrous
-
The role of dietary vitamin A in mechanisms of cataract development in the teleost lumpfish (Cyclopterus lumpus L) J. Fish Dis. (IF 2.5) Pub Date : 2023-12-01 Margret J. Deering, Hélène Paradis, Raahyma Ahmad, Adil S. Al-Mehiawi, Robert L. Gendron
Lumpfish (Cyclopterus lumpus L) are highly prone to cataract development in the wild and in culture. There is evidence that cataract in farmed fish is related to nutrition. However, both the nutrients and the mechanisms involved in cataract development in lumpfish are not clear. Here we investigated the mechanisms involved and the role of dietary vitamin A in cataract development in a cultured lumpfish
-
Gonadal neoplasia in Chinese gold barbs (Barbodes semifasciolatus) from a home aquarium J. Fish Dis. (IF 2.5) Pub Date : 2023-12-01 Justin M. Stilwell, Alvin C. Camus, Natalie K. Stilwell
CONFLICT OF INTEREST STATEMENT The authors declare no conflict of interests related to the performance or publication of the work described herein.
-
A case study of epizootic ulcerative syndrome (EUS) caused by Aphanomyces invadans in eastern mosquitofish (Gambusia holbrooki) from the headwaters of Charleston Harbor, South Carolina J. Fish Dis. (IF 2.5) Pub Date : 2023-11-30 Timothy J. Bruce, Carl C. Trettin, Zachary A. Noel, Alex T. Chow, Kylie Warden, Craig Roghair, Troy M. Farmer
1 INTRODUCTION Aphanomyces invadans is an oomycete (fungus-like eukaryotic microorganism), first documented in North America in the 1980s (Dykstra et al., 1986). This pathogen causes epizootic ulcerative syndrome (EUS) in many species of freshwater and brackish water fish. Initially discovered in Japan in 1971 (Egusa & Masuda, 1971), EUS is now widely distributed, with documented cases across 20 countries
-
Phenotypic and genomic characterization of a non-pathogenic Epilithonimonas ginsengisoli isolated from diseased farmed rainbow trout (Oncorhynchus mykiss) in Chile J. Fish Dis. (IF 2.5) Pub Date : 2023-11-29 Claudio D. Miranda, Rute Irgang, Christopher Concha, Rodrigo Rojas, Ruben Avendaño-Herrera
Flavobacterial infection associated with diseased fish is caused by multiple bacterial species within the family Flavobacteriaceae. In the present study, the Chilean isolate FP99, from the gills of a diseased, farmed rainbow trout (Oncorhynchus mykiss), was characterized using phenotypic and genomic analyses. Additionally assessed was pathogenic activity. Phylogenetic analysis based on 16S rRNA gene
-
Nocardiosis and other common diseases of cultured golden pompano (Trachinotus blochii) in Taiwan J. Fish Dis. (IF 2.5) Pub Date : 2023-11-28 Wei-Xiao Yan, Yu-Han Tseng, Wen-Rou Huang, Li-Wu Cheng, Pei-Chi Wang, Shih-Chu Chen
Golden pompano (Trachinotus blochii) is a carnivorous teleost cultured in the Asia-Pacific region. Fish culture in high densities and numbers results in disease outbreaks, causing huge economic losses. Here, we collected cultured golden pompanos from 2021 to 2022 and identified the pathogens isolated from the diseased fish. Out of a total of 64 clinical cases observed in both sea cages and fish ponds
-
Influence of colour background on anti-viral activity against viral haemorrhagic septicaemia virus in zebrafish regulated by circadian rhythm signalling pathway J. Fish Dis. (IF 2.5) Pub Date : 2023-11-28 Se-Hyun Cho, Se-Young Cho, Bipin Vaidya, Joseph Kwon, Duwoon Kim
The influence of colour background on the regulation of behavioural and physiological responses in zebrafish is widely recognized. However, its specific effect on virus infection in zebrafish remains unclear. This study aimed to explore the susceptibility of zebrafish to viral haemorrhagic septicaemia virus (VHSV) infection in relation to background colour, investigate the underlying mechanisms, and
-
Selection and characterization of ssDNA aptamer targeting Macrobrachium rosenbergii nodavirus capsid protein: A potential capture agent in gold-nanoparticle-based aptasensor for viral protein detection J. Fish Dis. (IF 2.5) Pub Date : 2023-11-28 Norazli Ghadin, Nur Afiqah Md Yusof, Baharum Syarul Nataqain, Nurul Hanun Ahmad Raston, Chen Fei Low
The giant freshwater prawn holds a significant position as a valuable crustacean species cultivated in the aquaculture industry, particularly well-known and demanded among the Southeast Asian countries. Aquaculture production of this species has been impacted by Macrobrachium rosenbergii nodavirus (MrNV) infection, which particularly affects the larvae and post-larvae stages of the prawn. The infection
-
Phenotypic and genomic insights into the pathogenicity and antimicrobial resistance of an Enterobacter roggenkampii strain isolated from diseased silver arowana (Osteoglossum bicirrhosum) J. Fish Dis. (IF 2.5) Pub Date : 2023-11-28 Yuerui Li, Zhongshi Sun
Enterobacter roggenkampii is an opportunistic pathogen that causes infections in a wide range of hosts. A bacterial strain named EOBSR_19 was isolated from diseased silver arowana, Osteoglossum bicirrhosum. This bacterium was identified as E. roggenkampii based on the phenotypic characteristics and sequence analysis of the16S rDNA and gyrB genes. Average nucleotide identity and phylogenetic analysis
-
Identifying the function of the PI3K-AKT pathway during the pathogenic infection of Macrobrachium rosenbergii J. Fish Dis. (IF 2.5) Pub Date : 2023-11-23 Fanbin Zhan, Shichun Zhou, Fei Shi, Qingqing Li, Li Lin, Zhendong Qin
The phosphoinositide-3-kinase/protein kinase b (PI3K-Akt) pathway is a signalling pathway based on protein phosphorylation and can be activated by a wide range of factors. To investigate the function of the PI3K-AKT signalling pathway in antibacterial immunity, we analysed the gene expression level of three key factors (PI3K, AKT and FoxO) and innate immune factors in immune tissues at different time
-
Experimental evidence of effective disinfectant to control the transmission of Micropterus salmoides rhabdovirus J. Fish Dis. (IF 2.5) Pub Date : 2023-11-22 Runzhen He, Ningyu Zhu, Xiaoming Chen, Qianrong Liang, Gaohua Yao, Quanquan Tian, Fan Zhou, Xueyan Ding
Micropterus salmoides rhabdovirus (MSRV) is a significant pathogen that causes high morbidity and mortality in largemouth bass, leading to enormous economic losses for largemouth bass aquaculture in China. The aim of this study was to investigate the efficacy of four disinfectants (potassium permanganate, glutaraldehyde, trichloroisocyanuric acid and povidone iodine) on MSRV, to control the infection
-
Immersion challenge of three salmonid species (family Salmonidae) with three multilocus sequence typing variants of Flavobacterium psychrophilum provides evidence of differential host specificity J. Fish Dis. (IF 2.5) Pub Date : 2023-11-16 Christopher Knupp, Thomas P. Loch
Bacterial coldwater disease (BCWD), caused by Flavobacterium psychrophilum, results in significant losses among multiple salmonid (family Salmonidae) species. Molecular epidemiology and serotyping studies have suggested that some variants are host specific; however, these associations have not been evaluated by cross-challenging fish species with putatively host-associated F. psychrophilum isolates
-
New Zealand rickettsia-like organism and Tenacibaculum maritimum vaccine efficacy study J. Fish Dis. (IF 2.5) Pub Date : 2023-11-17 D. Jaramillo, B. P. Busby, M. Bestbier, P. Bennett, Z. Waddington
A cohort of Chinook salmon juveniles was vaccinated, with an autogenous bivalent vaccine against New Zealand RLOs (NZ-RLO1) and Tenacibaculum maritimum. A proportion of the cohort was not vaccinated to act as controls. At smoltification, the fish were challenged with NZ-RLO1, NZ-RLO2, or T. maritimum. We found that challenge with T. maritimum by immersion in (7.5 × 105 cfu/mL of water) did not yield
-
A review of bacterial disease outbreaks in rainbow trout (Oncorhynchus mykiss) reported from 2010 to 2022 J. Fish Dis. (IF 2.5) Pub Date : 2023-11-15 Muhammed Duman, Soner Altun, Izzet Burcin Saticioglu, Jesús L. Romalde
Outbreaks of bacterial infections in aquaculture have emerged as significant threats to the sustainable production of rainbow trout (Oncorhynchus mykiss) worldwide. Understanding the dynamics of these outbreaks and the bacteria involved is crucial for implementing effective management strategies. This comprehensive review presents an update on outbreaks of bacteria isolated from rainbow trout reported
-
Tenacibaculum dicentrarchi produce outer membrane vesicles (OMV) that are associated with the cytotoxic effect in rainbow trout head kidney macrophages J. Fish Dis. (IF 2.5) Pub Date : 2023-11-10 Macarena Echeverría-Bugueño, Ruben Avendaño-Herrera
Tenacibaculum dicentrarchi is the second most important pathogen in Chilean salmon farming. This microorganism causes severe skin lesions on the body surface of farmed fish. The bacterium can also adhere to surfaces and form biofilm, survive in fish skin mucus, and possess different systems for iron acquisition. However, the virulence mechanisms are still not fully elucidated. Outer membrane vesicles
-
Aeromonas salmonicida, causative agent of salmonid furunculosis, isolated from the freshwater parasitic copepod, Salmincola californiensis J. Fish Dis. (IF 2.5) Pub Date : 2023-11-10 Crystal L. Herron, Natassia E. Ruse, Daniel D. Rockey, Justin L. Sanders, James T. Peterson, Carl B. Schreck, Michael L. Kent
Here, we provide evidence that the freshwater parasitic copepod, Salmincola californiensis, acts as a vector for Aeromonas salmonicida. While investigating the effects of S. californiensis on Chinoook salmon (Oncorhynchus tshawytscha), we tangentially observed that fish infected with the copepod developed furunculosis, caused by A. salmonicida. This occurred despite being reared in pathogen-free well