样式: 排序: IF: - GO 导出 标记为已读
-
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-11-22 Jin Ju Park,Ok-Hee Lee,Jie-Eun Park,Jaejin Cho
Multipotent mesenchymal stromal/stem cell (MSC) spheroids generated in three-dimensional culture are of considerable interest as a novel therapeutic tool for regenerative medicine. However, the lack of reliable methods for storing MSC spheroids represents a significant roadblock to their successful use in the clinic. An ideal storage medium for MSC spheroids should function as both a vehicle for delivery
-
Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-11-22 Liting Liu,Zirui Wang,Menghan Wang,Gang Zhao
During cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used
-
A Method to Freeze Skin Samples for Cryobanks: A Test of Some Cryoprotectants for an Endangered Deer. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-11-10 Laura Campos Cassavia Cintra de Oliveira,Gabrielle Queiroz Vacari,José Maurício Barbanti Duarte
The genetic diversity of endangered deer species, such as Mazama jucunda, can be preserved with the help of somatic cell cryopreservation. This procedure allows obtaining several cells from the individual even after its death, which is very important for applications in reproductive biotechnologies. This study's objective was to test cryopreservation protocols of skin fragments of M. jucunda, using
-
Increase in Serum Potassium Levels After Refrigerated Storage: A Component of Blood Clot Contaminates the Serum Layer Over the Separator Gel. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-11-09 Kohsuke Yoshida,Hiromi Tani,Sae Nobuhara,Shinji Yamamoto
Objectives: The aim of this study was to determine the cause of elevated serum potassium levels when blood collection tubes containing separating gel are stored under refrigeration. Methods: Fifty-seven hospitalized patients and 11 healthy volunteers were recruited. Venous blood samples were obtained using Insepac II, Neotube, and Venoject® II, without anticoagulant. After centrifugation under different
-
The Deceased, Public Health, and Research: Proposing Legal Reforms. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-11-09 Isabel Baltzan,Bartha Maria Knoppers,Elisheva Tamar Anne Nemetz,Jordan Lerner-Ellis,Alexander Bernier,Karen Devon
There is little guidance concerning biomedical research using tissues from deceased individuals. Unique ethical and legal challenges gained visibility during the coronavirus disease 2019 (COVID-19) pandemic, when important studies using genome sequencing required access to biological materials from deceased individuals. These studies proposed to determine whether specific genomic profiles were associated
-
Homology Identification and Cross-Contamination Analysis: A Method for Evaluating the Quality of Biological Samples Stored in a Biobank Using the Advanta Sample ID Genotyping Panel. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-10-27 Chao Wang,Zebin Hu,Xiaoyan Zhang,Midie Xu,Weixiang Shen,Lili Du,Menghong Sun,Hengjun Gao
Biological samples are important resources for scientific research. These samples are stored in biobanks over years until needed, and some of them can never be retrieved if they are improperly stored, causing them to be wasted. Thus, they are priceless, and they should be used correctly and effectively. Sample quality substantially affects biomedical research results. However, sample misidentification
-
Biospecimen Qualification in a Clinical Biobank of Urological Diseases. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-10-25 Olga Kofanova,Sangita Paul,Achilleas Pexaras,Camille Bellora,Ala Petersons,Margaux Schmitt,Mohamad Baker Berjaoui,Yazan Qaoud,Miran Kenk,Heidi Wagner,Neil Fleshner,Fay Betsou
Development of novel biomarkers for diagnosis of disease and assessment of treatment efficacy utilizes a wide range of biospecimens for discovery research. The fitness of biospecimens for the purpose of biomarker development depends on the clinical characteristics of the donor and on a number of critical and potentially uncontrolled pre-analytical variables. Pre-analytical factors influence the reliability
-
Relationship Between Amplicon Size and Heat Conditions in Polymerase Chain Reaction Detection of DNA Degraded by Autoclaving. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-10-23 Naoki Harikai,Yuki Takada,Misaki Saito,Kazumasa Zaima,Kazufusa Shinomiya
This study examined the influence of heat exposure on DNA samples during polymerase chain reaction (PCR) detection. In this study, λDNA samples, as model DNA, were exposed to 105°C for 3-90 minutes or to 105°C-115°C for 15 minutes by autoclaving. The exposed samples were subjected to real-time PCR using nine primer sets with amplicon sizes of 45-504 bp. Regarding DNA samples exposed to 105°C by autoclaving
-
Granulocyte-Macrophage Colony-Stimulating Factor Cytokine Addition After the Freeze-Thawing Process Improves Human Sperm Motility and Vitality in Asthenoteratozoospermia Patients. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-10-06 Elham Hosseini,Parivash Afradiasbagharani,Masoud Mohammadian,FatemehSadat Amjadi,Maryam Tabatabaei,Fateme Tanhaye Kalate Sabz,Zahra Zandieh
The cryopreservation-thawing process of spermatozoa cells has negative impacts on their structure, function, and fertility parameters, which are known as cryoinjury. Asthenozoospermia patients are more susceptible to cryoinjury. Granulocyte-macrophage colony-stimulating factor (GM-CSF) increases sperm glucose uptake via the induction of glucose transporters, resulting in increased sperm motility. This
-
Biobanking: A Cornerstone of Biodigital Convergence. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-10-01 Jörg Geiger,Brent Schacter,Francois Coallier
-
Review of Rewarming Methods for Cryopreservation. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-09-26 Jiaji Pan,Qijin Zeng,Ke Peng,Yulin Zhou,Zhiquan Shu
Cryopreservation is the most effective technology for the long-term preservation of biological materials, including cells, tissues, and even organs in the future. The process of cooling and rewarming is essential to the successful preservation of biological materials. One of the critical problems in the development of cryopreservation is the optimization of effective rewarming technologies. This article
-
Collection, Handling, and Preservation of Wild Bird Semen: Current Status, Challenges, and Perspectives. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-09-12 Luana Grasiele Pereira Bezerra,Radan Elvis Matias de Oliveira,Pierre Comizzoli,Alexandre Rodrigues Silva
Semen preservation is a significant biotechnology used to safeguard the genetic material of birds, especially those with declining populations, through biobanking. However, there are limited reports on the successful chilling or cryopreservation of wild bird semen. In general, these techniques are not yet well-established for several species of wild birds and pose several challenges such as the need
-
In Response to "Establishing Biobanking in Medical Curricula-The Education Program 'Precision Medicine International' (eduBRoTHER)" in Biopreservation and Biobanking, Published on November 11, 2022 by Seidler D., et al. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-09-07 Fayek Elkhwsky,Amal Allam,Zisis Kozlakidis,Amal Shaikhah,Asmaa Abd Elhameed
-
Biobank Education for Future Physicians: Training Medical Students Through Student Research Association Networks. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-09-06 Mustafa Karataş,Muhammet Ekin Azbazdar,Melis Camkiranlar,Sanem Tercan-Avci,Neşe Atabey
Research biobanks have become crucial collaborators in a variety of basic and clinical research projects with comprehensive biological sample collection and associated data storage. Medical students, who are the most important stakeholders of biobanks as future physicians, need to be trained in biobanking; however, there is no consensus on how to include it in formal education. This study aimed to
-
The Influences of Cryopreservation Methods on RNA, Protein, Microstructure and Cell Viability of Skeletal Muscle Tissue. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-08-18 Xiang Huang,Jingjing Jiang,Junmin Shen,Ziying Xu,Fangyan Gu,Jinlian Pei,Licheng Zhang,Peifu Tang,Pengbin Yin
Background: Different experiments require different sample storage methods. The commonly used preservation methods in biobank practice cannot fully meet the multifarious requirements of experimental techniques. Programmable controlled slow freezing (PCSF) can maintain the viability of tissue. In this study, we hypothesized that PCSF-preserved samples have potential advantages in matching subsequent
-
Egg Yolk-Supplemented Tris-Citric Acid Extender Improves the Prefreezing and Post-Thaw Sperm Quality Indices of Guinea Pig (Cavia porcellus) Epididymal Spermatozoa. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-08-17 Diego A Galarza,Wilson León-Machuca,Jorge X Samaniego,Rubén Carrera,Fernando P Perea,Mauricio Duma,Julián Santiago-Moreno
This study aimed to assess the suitability of egg yolk (EY) supplementation to a tris-citric acid-based extender on cryosurvival of guinea pig (Cavia porcellus) epididymal spermatozoa. Two synthetic-based extenders, tris-citric acid-glucose plus 20% EY (TCG-EY) and tris-citric acid-fructose (TCF) both with 5% glycerol, were compared. Thirty-two epididymides were recovered from 16 adult guinea pig males
-
Variable Control and Its Influence Before Urine Sample Analysis in a Field Environment. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-08-17 Jingjing Jiang,Hanxuan Liu,Wenfeng Ni,Manli Zhang,Fangyan Gu,Jinlian Pei,Yan Wang,Yaping Tian
Background and Objectives: The aim of the study was to store urine samples at different temperatures and humidity levels and analyze common biochemical test results and point-of-care testing (POCT) indicators according to different storage times and evaluate whether the samples should be centrifuged to study the best storage conditions for urine samples. Methods: Random midstream urine samples (100 mL)
-
Toward Professionalism of Biobanking in China: A Survey on Working Status, Career Development, Challenges, and Prospects of Biobankers. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-08-15 Xuexun Zhou,Qian Li,Qian Zhao,Xiaoyan Zhang,Yanping Xu,Puyi Qian,Frank Liu Gao,Io Hong Cheong,Dan Guo,Shihui Ma,Huan Chen,Sijian Liu,Weiye Wang,Xiaonan Kang,Menghong Sun,Yajun Yang,Qubo Chen,Hengjun Gao
Biobanking has become an increasingly important activity to provide resources for medical research support. In China, establishing and maintaining a biobank have been the latest trend in a research hospital. However, biobanking is still an emerging young field in terms of professionalization and professionalism. The development of professionalization in biobanking faces many challenges involving the
-
Recovering Spermatogenesis By Protected Cryopreservation Using Metformin and Transplanting Spermatogonial Stem Cells Into Testis in an Azoospermia Mouse Model. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-08-15 Alieh Bashghareh,Tayebeh Rastegar,Peyman Modarresi,Shokoofeh Kazemzadeh,Maryam Salem,Azim Hedayatpour
Cryopreservation and transplantation of spermatogonial stem cells (SSCs) may serve as a new method to restore male fertility in patients undergoing chemotherapy or radiotherapy. However, SSCs may be damaged during cryopreservation due to the production of reactive oxygen species (ROS). Therefore, different antioxidants have been used as protective agents. Studies have shown that metformin (MET) has
-
An Efficient Extraction Method Allowing the Genetic Evaluation of Host DNA from Samples Collected for Virus Infection Diagnosis in Viral Transport Medium. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-08-09 Renan C Sbruzzi,Mariléa F Feira,Nathan A Cadore,Giovanna C Giudicelli,Thayne W Kowalski,Tatiana S Gregianini,José A B Chies,Fernanda S L Vianna
Introduction: During the COVID-19 pandemic, an extraordinary number of nasopharyngeal secretion samples inoculated in viral transport medium (VTM) were collected and analyzed to detect SARS-CoV-2 infection. In addition to viral detection, those samples can also be a source of host genomic material, providing excellent opportunities for biobanking and research. Objective: To describe a simple, in-house-developed
-
The Effect of Blood Clots on the Quality of RNA Extracted from PAXgene Blood RNA Tubes. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-08-03 Rong Tang,Ling Zhu,Ping Zhu,Ru Yin,Chunxia Zheng
Background: PAXgene® Blood RNA tubes are routinely used in clinical research and molecular biology applications to preserve the stability of RNA in whole blood. However, in practice, blood clots are occasionally observed after blood collection and are often ignored. Currently, there are few studies on whether blood clots affect the quality of RNA extracted from these tubes. Materials and Methods: Fifteen
-
Biobanking in the Asia-Pacific Region: The Challenges of International Biospecimen Sharing. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-08-01 Annette Schmid,Zisis Kozlakidis,Marianna Bledsoe
-
Heterologous Seminal Plasma Reduces the Intracellular Calcium and Sperm Viability of Cryopreserved Stallion Spermatozoa. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-07-19 Thirumala Rao Talluri,Arumugam Kumaresan,Nilendu Paul,Kamaraj Elango,Kathan Raval,Pradeep Nag,Ram Avtar Legha,Yash Pal
Despite the vital role of seminal plasma (SP) in maintaining sperm function and aiding gamete interaction in many species, SP is usually removed before cryopreservation of stallion sperm to improve cryosurvival of sperm. The present study assessed if the vital sperm functional parameters of genetically superior stallions producing poor quality semen can be enhanced by the supplementation of heterologous
-
Re: "Protective Effect of Cerium Oxide Nanoparticles on Human Sperm Function During Cryopreservation," by Hosseinmardi et al. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-07-18 Zahra Asadi,Asad Vaisi-Raygani,Faranak Aghaz
-
Insight into Increased Recovery and Simplification of Genomic DNA Extraction Methods from Dried Blood Spots. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-07-07 Kiara Lee,Anubhav Tripathi
There is no consensus on how to perform the manual extraction of nucleic acids from dried blood spots (DBSs). Current methods typically involve agitation of the DBSs in a solution for varying amounts of time with or without heat, and then purification of the eluted nucleic acids with a purification protocol. We explored several characteristics of genomic DNA (gDNA) DBS extraction such as extraction
-
Metrics for the Evaluation of Bioresources: Tell Us the Whole Story. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-06-01 Marianna J Bledsoe,Peter H Watson,William E Grizzle
-
The Effect of Adding Different Levels of Reduced Glutathione to Extender on the Quality of Cooled Ring-Necked Pheasant Semen. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-05-23 Sumiyyah Zuha,Bushra Allah Rakha,Shamim Akhter,Muhammad S Ansari,Kainat Waseem
Aim: Artificial propagation of ring-necked pheasant through semen preservation is of significance, as this species is facing enormous threats in its natural habitat. Semen preservation inevitably induces oxidative stress, and exogenous antioxidants need to be investigated for the preservation of ring-necked pheasant semen. Therefore, the current study was conducted to investigate the role of glutathione
-
Developing Policy for the Healthy Life Trajectories Initiative: Going from National to International. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-05-16 Dimitri Patrinos,Erika Kleiderman,William Fraser,Ma'n H Zawati,
Background: Scientific research is becoming an increasingly collaborative and global venture. The Healthy Life Trajectories Initiative (HeLTI), for instance, is an international Developmental Origins of Health and Disease research collaboration developed to address the increasing burden of noncommunicable diseases around the world. It comprises four separate but harmonized cohort trials in Canada,
-
A New Medium (HistoCold) for Surgical Specimens Preserving to Improve the Preanalytic Issues in Histopathological Samples Handling: Morphologic and Antigenic Analysis. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-05-16 Martina Mandarano,Cristina Pelliccia,Laura Tomasello,Emanuele Caselli,Claudia Floridi,Elisabetta Loreti,Francesco Barberini,Antonio Rulli,Alessio Gili,Rossella Potenza,Francesco Puma,Emanuele Rosati,Annibale Donini,Adolfo Petrina,Paolo Baccari,Rachele Del Sordo,Renato Colella,Guido Bellezza,Angelo Sidoni
Introduction: The onset of precision medicine has led to the integration of traditional morphologic tissues evaluation with biochemical and molecular data for a more appropriate pathological diagnosis. The preanalytic phase and, particularly, timing of cold ischemia are crucial to guarantee high-quality biorepositories of formalin-fixed paraffin-embedded (FFPE) tissues for patients' needs and scientific
-
Use of Antifreeze Protein from Tenebrio molitor (TmAFP) in Vitrification of In Vitro-Produced Bovine Embryos: An Ultrastructural Study. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-05-16 Rafael Artur da Silva Júnior,Raquel Desenzi,Millena Mary da Silva Ramires,Andreia Fernandes de Souza,Mariana Aragão Matos Donato,Christina Alves Peixoto,Cláudio Coutinho Bartolomeu,André Mariano Batista
The objective of this study was to evaluate the effects of different concentrations of antifreeze protein (AFP) extracted from the larva of the beetle, Tenebrio molitor (TmAFP), on vitrification of in vitro-produced bovine embryos. In vitro-produced blastocysts were divided into three experimental groups and vitrified using a cryotop. TmAFP was added to the equilibrium solution (ES) and vitrification
-
Achieving Procedural Parity in Managing Access to Genomic and Related Health Data: A Global Survey of Data Access Committee Members. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-05-16 Jonathan Lawson,Vasiliki Rahimzadeh,Jinyoung Baek,Edward S Dove
Data access committees (DACs) are critical players in the data sharing ecosystem. DACs review requests for access to data held in one or more repositories and where specific constraints determine how the data may be used and by whom. Our team surveyed DAC members affiliated with genomic data repositories worldwide to understand standard processes and procedures, operational metrics, bottlenecks, and
-
Fortification of Extender with Basella rubra Fruit Extract Enhances the Cryosurvival of Ram Semen. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-05-08 Mohsin Ali,Sadia Suleman,Iram Inayat,Syeda Nadia Ahmad,Muhammad Ali Kanwal,Khawaja Raees Ahmad,Saira Siddique,Rabiyah Ali,Saima Matloob,Hafiz Abdul Sattar,Muhammad Atif Kamran
This study aimed to evaluate the impact of Basella rubra fruit extract (BR-FE) on cryopreserved ram sperm's motility, velocity, and membrane integrity. Thirty ejaculates collected from 3 fertile rams (10 from each) were diluted with semen dilution extender (SDE) in a ratio (1:2) and centrifuged to remove 50% supernatant. The remaining sample was mixed with semen cryopreservation extender (SCE) in 1:4
-
Effects of Repeated Freeze and Thaw Cycles on the Genome-Wide DNA Methylation Profile of Isolated Genomic DNA. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-04-18 Verena Kopfnagel,Norman Klopp,Inga Bernemann,Nataliia Nizhegorodtseva,Rory Wilson,Raphael Gronauer,Martin Seifert,Thomas Illig
The characterization of DNA methylation patterns to identify epigenetic markers for complex human diseases is an important and rapidly evolving part in biomedical research. DNA samples collected and stored in clinical biobanks over the past years are an important source for future epigenetic studies. Isolated gDNA is considered stable when stored at low temperatures for several years. However, the
-
Impact of Sample Processing and Storage Conditions on RNA Quality of Fresh-Frozen Cancer Tissues. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-04-11 Yuan-Yuan Chen,Qiu-Yue Han,Qiong-Yuan Chen,Wen-Jun Zhou,Jian-Gang Zhang,Xia Zhang,Aifen Lin
Background: A biobank is a central resource that supports basic and clinical research. RNA quality of fresh-frozen tissue specimens in the biobank is highly associated with the success of downstream applications. Therefore, it is very important to evaluate the impact of tissue processing and storage conditions on RNA quality. Methods: A total of 238 surgically removed tissue specimens, including esophagus
-
Readiness for Artificial Intelligence in Biobanking. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-04-01 Gregory H Grossman,Marianne K Henderson
-
It's a Small World After All…or Is It? Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-04-01 Clare M Allocca,Elne Conradie,Koh Furuta,Diane McGarvey,Alison Parry-Jones
-
Researchers' Perspectives Regarding Ethical Issues of Biobank Research in the Arab Region. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-03-23 Maha E Ibrahim,Latifa Adarmouch,Alya Elgamri,Samar Abd ElHafeez,Zeinab Mohammed,Fatma Abdelgawad,Eman H Elsebaie,Ahmed Samir Abdelhafiz,Ehsan Gamel,Karima El Rhazi,Asmaa Abdelnaby,Mamoun Ahram,Henry Silverman
Background: The recent expansion of genomic biobank research in the Arab region in the Middle East North Africa has raised complex ethical and regulatory issues. However, there is a lack of studies regarding the views of Arab researchers involved in such research. We aimed to assess the perceptions and attitudes of Arab researchers regarding these issues in biobank research. Methods: We developed a
-
-
The Combination of Estradiol and N-Acetylcysteine Reduces Ischemia-Reperfusion Injuries of Mice Autografted Ovarian Tissue. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-02-24 Fatemeh Ebrahimi,Saeed Zavareh,Meysam Nasiri
Ischemia-reperfusion injuries are important issues after ovarian tissue transplantation (OTT). Our study examined the effects of N-acetylcysteine (NAC) and estradiol (E2) on mouse ovarian autografts. Mice (6-8 weeks) were divided into ovarian autograft as follows: Control: fresh OTT; Sham: cryopreserved/warmed OTT; NAC: cryopreserved/warmed OTT with NAC treatment; E2: cryopreserved/warmed OTT with
-
Dimethyl Sulfoxide-Free Cryopreservation of Differentiated Human Neuronal Cells. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-02-23 Kenji Yamatoya,Yuya Nagai,Naozumi Teramoto,Woojin Kang,Kenji Miyado,Kazuya Nakata,Tohru Yagi,Yoshitaka Miyamoto
In recent years, cells provided by cell banks and medical facilities have been used for cell therapy, regenerative therapy, and fundamental research. Cryopreservation is an effective means of maintaining stable cell quality over a long period of time. The slow freezing method is most suitable for processing many human cells isolated simultaneously from organs and tissues, but it is necessary to develop
-
Genetically Engineered Exosomes as a Potential Regulator of Th1 Cells Response in Rheumatoid Arthritis. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-03-13 Zheng Ren,Xiuxin Liu,Elham Abdollahi,Fataneh Tavasolian
Background: Rheumatoid arthritis is a long-lasting inflammatory disease that usually involves joints, but it can also affect other organs, including the skin and lungs. In this case, it is important to maintain a balance between beneficial pro-inflammatory activity and harmful overactivation of the T helper cells (Th). We strive to investigate in this study the possibilities for the effect of mesenchymal
-
A Comparative Study of Three Nucleic Acid Integrity Assay Systems Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-02-01 Xinyi Lu, Yulian Wei, Jing Sun, Bo Xiao, Xiaoyan Zhang, Wanhua Li, Ying Chen, Fengye Lin, Li Zhang, Yan Wang, Jing Zheng, Lingyan Zheng, Xingzhi Zhou, Qubo Chen
Background: The measurement of nucleic acid quality, especially the analysis of integrity, is a key step for many downstream experiments in biomedical research and quality control of biomaterials. General gel electrophoresis is a traditional method for nucleic acid integrity analysis. Currently, more electrophoresis techniques are becoming standardized and automated operations with higher precision
-
Establishment of a Standard Tongue Coating Collection Method for Microbiome Studies Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-02-02 Xuan Zeng, Huihui Jin, Chuyang Wang, Man Li, Ruohan Wang, Wanhua Li, Fengye Lin, Ying Chen, Weicheng Chen, Xiaoting Huang, Jun Liu, Mingzhu Zheng, Xuanting Jiang, Qubo Chen
Objective: Recently, researchers have been focusing on characterizing the tongue coating microbiome from patients with digestive tract disease. However, to the best of our knowledge, the tongue coating collection methods have not been standardized until now. This article focuses on bridging this gap by exploring and validating the conditions suitable for the collection of tongue coating samples.
-
Priorities and Future of the ISBER Standards Community of Practice and Pediatric Special Interest Group. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-02-01 Clare Allocca,Mieke De Wilde,Rania Labib
-
Biopreservation and Biobanking 2022: The Year in Review. Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-02-01 Marianna J Bledsoe,Dayong Gao
-
Influence of Bacterial Contamination and Antibiotic Sensitivity on Cryopreserved Sperm Quality of Indian Red Jungle Fowl Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-01-18 Bushra A. Rakha, Zartasha Zafar, Muhammad S. Ansari, Shamim Akhter, Saima Qadeer, Ali Akhter, Kainat Waseem, Julian Santiago-Moreno
Aims: Bacterial contamination may occur in feces during collection and processing of semen. Bacteria not only compete for nutrients with spermatozoa but also produce toxic metabolites and endotoxins and affect sperm quality. The aim of the present study was to investigate the effect of antibiotic supplementation on the sperm quality of Indian red jungle fowl, estimation and isolation of bacterial species
-
The Effects of Glycerophospholipid Nanomicelles on the Cryotolerance in the Frozen–Thawed Rooster Sperm Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-01-13 Zahra Farrokhi, Mohsen Sharafi, Maryam Hezavehei, Ali Torabi, Maryam Shahverdi, Shaban Rahimi
Semen banking is an efficient method of artificial insemination for commercial breeders. However, the cryopreservation process induces severe damages to plasma membranes, which lead to reduced fertility potential of thawed sperm. The replacement of membrane lipids with oxidized membrane lipids repairs the cell membrane and improves its stability. The aim of this study was to investigate the effects
-
The Influence of Preanalytical Biospecimen Handling on the Measurement of B Vitamers, Amino Acids, and Other Metabolites in Blood Biopreserv. Biobank. (IF 1.6) Pub Date : 2023-01-09 Kara A. Michels, Stephanie J. Weinstein, Paul S. Albert, Amanda Black, Michelle Brotzman, Norma A. Diaz-Mayoral, Nicole Gerlanc, Wen-Yi Huang, Joshua N. Sampson, Alaina Shreves, Per Magne Ueland, Kathleen Wyatt, Nicolas Wentzensen, Christian C. Abnet
Introduction: Sample handling can influence biomarker measurement and introduce variability when combining data from multiple studies or study sites. To inform the development of blood collection protocols within a multisite cohort study, we directly quantified concentrations of 54 biomarkers in blood samples subjected to different handling conditions.
-
Assessment of 2-Year Storage Conditions on Protein, RNA, and DNA in Unstained Human Tissue Sections, Including a Novel Multiplex Digital Gene Expression Profiling Method with Implications for Biobanking Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Colleen Ramsower, Lee Wisner, Katie Zellner, Betty Glinsmann-Gibson, Brandon Larsen, Michael McGrath, Alanna Maguire, Lisa Rimsza
Background: Formalin-fixed, paraffin-embedded (FFPE) tissues are a valuable resource for clinical and basic science research. Paraffin blocks and the resulting unstained sections (USS) are often stored for years before being used. Previous studies have evaluated the effects of time, temperature, humidity, and inert gases on preservation of USS; however, no study has examined all four variables together
-
The Role of TNF-α and Its Target HSP-70 in Triggering Apoptosis in Normozoospermic and Non-Normozoospermic Samples Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Seda Karabulut, Duygu Gürsoy Gürgen, Pelin Kutlu, İlknur Keskin
Objective: Semen analysis is performed as one of the screening tests for infertility, including motility, morphology, and concentration observation. We aimed to investigate the expression rates of tumor necrosis factor-α (TNF-α) and heat shock protein (HSP)-70 as two opposite affectors of apoptosis in men with normal semen parameters and abnormal parameters to find the possible effect of this pathway
-
Nonsurgical Embryo Recovery as a Feasible Tool for Supporting Embryo Biobanks of Locally Adapted Brazilian Sheep and Goats Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Jeferson F. Fonseca, Gabriel B. Vergani, Monalisa S.D. Lima, Kleibe M. Silva, Alexandre W.U. Monteiro, Alexandre F. Ramos, Bruna R.C. Alves, Joanna M.G. Souza-Fabjan, Maria E.F. Oliveira, Ribrio I.T.P. Batista
This study assessed the outcomes of nonsurgical embryo recovery (NSER) after superovulation (SOV) in five locally adapted Brazilian breeds of sheep and goats. The objective was to evaluate the feasibility and efficiency of using SOV combined with a less-invasive embryo collection technique for supplying the Brazilian animal gene bank with germplasm from specific genotypes of interest. Morada Nova (n = 20)
-
Dimethylformamide Preserves the Integrity of Cryopreserved Goat Semen in a Soybean Lecithin-Based Extender Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Robespierre Augusto Joaquim Araújo Silva, Millena M. Monteiro, Joana A.S. Costa, Flávio C.C. Mergulhão, Pedro L.J. Monteiro, Diogo M.F. Silva, Antônio S. Santos Filho, Andréia F. Souza, André M. Batista, Maria Madalena P. Guerra
This study investigated the cryoprotectant effects of dimethylformamide (DMF), ethylene glycol (EG), and dimethyl sulfoxide (DMSO) as substitutes for glycerol (GLY) in a soybean lecithin (SL)-based extender in the cryopreservation of buck sperm. In this study, the semen of three Saanen bucks was individually extended in SL supplemented with 5% GLY (control), DMF, EG, or DMSO. After this, the extended
-
Effect of Milk Type Subjected to Different Heat Treatments on Cryo-Survivability and In Vivo Fertility of Buffalo (Bubalus bubalis) Spermatozoa in a Milk-Based Extender Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Haseeb Ali Tariq, Arslan Tariq, Nasim Ahmad, Muhammad Nadeem, Amjad Riaz
Oxidative stress is a major contributory factor to cellular damage during semen cryopreservation and results in a decreased fertilizing capacity of cryopreserved bull sperm. The inclusion of exogenous antioxidants sometimes exerts deleterious effects on sperm quality. Thus, enhancing the endogenous production of antioxidants is a requirement. This study aimed to investigate the effect of milk type
-
Combined Effect of Flaxseed Oil and Vitamin E Supplementation During Bull Semen Cryopreservation on Sperm Characteristics Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Chongshan Yuan, Hongtao Wang, Xichun Li, Hongyu Liu, Jing Zhao, Wenfa Lu, Jun Wang
The present study was performed to investigate the effects of supplementing flaxseed oil (FO) or vitamin E (VE) or their combination to an extender for Simmental bull semen cryopreservation. In experiment 1, different concentrations of FO (0, 10, 100, and 1000 ng/mL) and VE (0.05, 0.1, and 0.2 mg/mL) were added to the extenders. In experiment 2, FO, VE, and FO + VE were added and a control group was
-
Optimization of Follicle Isolation for Bioengineering of Human Artificial Ovary Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Jing Chen, Evgenia Isachenko, Wanxue Wang, Xinxin Du, Mengying Wang, Gohar Rahimi, Peter Mallmann, Vladimir Isachenko
Background: A functional artificial ovary is a promising strategy to recover fertility and restore endocrine function in cancer patients. The aim of this study is to optimize the follicle isolation protocol for cryopreserved human ovarian tissues.
-
Protective Effects of Trehalose and Pentoxifylline on Goat Sperm Exposed to Chilling-Freezing Process Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Parisa Nazari, Abbas Farshad, Yasser Hosseini
Freezing of sperm is known as an important part of assisted reproduction. However, many studies have illustrated that cryopreservation negatively affects the quality and fertility rate of sperm. This study aimed to evaluate the effects of trehalose and pentoxifylline (PTX) in diluents on cooled and frozen-thawed Markhoz goat sperm. Preassessed samples were pooled and diluted with a basic diluent using
-
Effect of Ascorbic Acid and Proline Amino Acid Supplementations on Cryosurvival and Fertility Rates of Cryopreserved Honey Bee (Apis mellifera) Semen Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Aziz Gül, Cengiz Yildiz, Nurdan Coşkun Çetin, Oguz Kaan Yalçin
This study investigated the effect of ascorbic acid (vitamin C) and proline amino acid alone or together on the quality and fertility of frozen/thawed honey bee spermatozoa. The experiments were designed to compare a single ascorbic acid, a single proline amino acid, and different combinations of ascorbic acid with proline amino acid on the cryopreservation of honey bee semen based on sperm motility
-
Morphological, Ultrastructural, and Immunocytochemical Characterization and Assessment of Puma (Puma concolor Linnaeus, 1771) Cell Lines After Extended Culture and Cryopreservation Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Gabriela P.O. Lira, Alana A. Borges, Matheus B. Nascimento, Leonardo V.C. Aquino, Luiz F.M.P. Moura, Herlon V.R. Silva, Leandro R. Ribeiro, Alexandre R. Silva, Alexsandra F. Pereira
Cell lines are valuable tools to safeguard genetic material from species threatened with extinction that is mainly due to human action. In this scenario, the puma constitutes a species whose population is being rapidly reduced in the ecosystems it inhabits. For the first time, we characterized puma skin-derived cell lines and assessed these cells after extended culture (experiment 1) and cryopreservation
-
Establishment of a New Cryopreservation Solution for Chimeric Antigen Receptor T Cells Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-16 Qulai Tang, Lixing Gu, Binquan Zhou, Jiangzhou Shi, Han Wu, Haichuan Zhu, Yao Xu, Tongcun Zhang
Preservation and transportation are essential for the clinical application of chimeric antigen receptor T (CAR-T) cells. This study aimed to optimize a cryopreservation solution for CAR-T cells and evaluate the antitumor efficiency of CAR-T cells using this optimized solution in vitro and in vivo. First, the stability of the cryopreservation solution for CAR-T infusion was detected by the L27 (37)
-
Attitudes of the Public Toward Consent for Biobank Research in Japan Biopreserv. Biobank. (IF 1.6) Pub Date : 2022-12-19 Masanori Oikawa, Yoshiyuki Takimoto, Akira Akabayashi
Background: Parallel to the rapid advancement of biological and information technologies, the role and forms of biobank research have been constantly changing. The ethical, legal, and social implications of consent in biobank research are in a state of flux. This study aimed to clarify current Japanese public preferences regarding the consent model and explore how public attitudes are determined.