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Engineering models of head and neck and oral cancers on-a-chip Biomicrofluidics (IF 3.2) Pub Date : 2024-03-06 Mauricio Goncalves da Costa Sousa, Sofia M. Vignolo, Cristiane Miranda Franca, Jared Mereness, May Anny Alves Fraga, Alice Corrêa Silva-Sousa, Danielle S. W. Benoit, Luiz Eduardo Bertassoni
Head and neck cancers (HNCs) rank as the sixth most common cancer globally and result in over 450 000 deaths annually. Despite considerable advancements in diagnostics and treatment, the 5-year survival rate for most types of HNCs remains below 50%. Poor prognoses are often attributed to tumor heterogeneity, drug resistance, and immunosuppression. These characteristics are difficult to replicate using
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Digital microfluidics methods for nucleic acid detection: A mini review Biomicrofluidics (IF 3.2) Pub Date : 2024-03-05 Youqiang Xing, Yan Wang, Xiang Li, Shangran Pang
Many serious infectious diseases have occurred throughout human history. Rapid and accurate detection as well as the isolation of infected individuals, through nucleic acid testing, are effective means of containing the spread of these viruses. However, traditional nucleic acid testing methods rely on complex machines and specialized personnel, making it difficult to achieve large-scale, high-throughput
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A miniaturized culture platform for control of the metabolic environment Biomicrofluidics (IF 3.2) Pub Date : 2024-03-01 Marta K. Orlowska, James R. Krycer, Janice D. Reid, Richard J. Mills, Michael R. Doran, James E. Hudson
The heart is a metabolic “omnivore” and adjusts its energy source depending on the circulating metabolites. Human cardiac organoids, a three-dimensional in vitro model of the heart wall, are a useful tool to study cardiac physiology and pathology. However, cardiac tissue naturally experiences shear stress and nutrient fluctuations via blood flow in vivo, whilst in vitro models are conventionally cultivated
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Photo-addressable microwell devices for rapid functional screening and isolation of pathogen inhibitors from bacterial strain libraries Biomicrofluidics (IF 3.2) Pub Date : 2024-02-29 Niloy Barua, Ashlee M. Herken, Natalie Melendez-Velador, Thomas G. Platt, Ryan R. Hansen
Discovery of new strains of bacteria that inhibit pathogen growth can facilitate improvements in biocontrol and probiotic strategies. Traditional, plate-based co-culture approaches that probe microbial interactions can impede this discovery as these methods are inherently low-throughput, labor-intensive, and qualitative. We report a second-generation, photo-addressable microwell device, developed to
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Harnessing the power of Microscale AcoustoFluidics: A perspective based on BAW cancer diagnostics Biomicrofluidics (IF 3.2) Pub Date : 2024-02-29 C. L. Harshbarger
Cancer directly affects one in every three people, and mortality rates strongly correlate with the stage at which diagnosis occurs. Each of the multitude of methods used in cancer diagnostics has its own set of advantages and disadvantages. Two common drawbacks are a limited information value of image based diagnostic methods and high invasiveness when opting for methods that provide greater insight
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Manually pressurized droplet digital PCR chip for rapid SARS-CoV-2 diagnostics Biomicrofluidics (IF 3.2) Pub Date : 2024-02-27 Pinja Elomaa, Tuomas Ojalehto, Darshan Kumar, Ville Jokinen, Päivi Saavalainen
Droplet digital PCR (ddPCR) is a technique in which PCR reaction is divided into thousands of nanoliter-sized droplets and has proven to be a great tool in virus diagnostics. Compared to the gold standard system quantitative real-time PCR (RT-qPCR), ddPCR functions particularly well when dealing with samples with low template counts, such as viral concentration. This feature makes the technique suitable
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Review on bile dynamics and microfluidic-based component detection: Advancing the understanding of bilestone pathogenesis in the biliary tract Biomicrofluidics (IF 3.2) Pub Date : 2024-02-16 Tao Peng, Chenxiao Zhou, Zhexin Zhang, Yingying Liu, Xiaodong Lin, Yongqing Ye, Yunlong Zhong, Ping Wang, Yanwei Jia
Bilestones are solid masses found in the gallbladder or biliary tract, which block the normal bile flow and eventually result in severe life-threatening complications. Studies have shown that bilestone formation may be related to bile flow dynamics and the concentration level of bile components. The bile flow dynamics in the biliary tract play a critical role in disclosing the mechanism of bile stasis
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Microfluidic devices integrated with plasmonic nanostructures for sensitive fluorescent immunoassays Biomicrofluidics (IF 3.2) Pub Date : 2024-02-16 Xuefeng Xu, Guangyang Li, Lingyue Xue, Shurong Dong, Jikui Luo, Zhen Cao
The robust identification and quantification of various biomarkers is of utmost significance in clinical diagnostics and precision medicine. Fluorescent immunoassays are widely used and considered as a gold standard for biomarker detection due to their high specificity and accuracy. However, current commercial immunoassay tests suffer from limited detection sensitivity and complicated, labor-intensive
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Surface-dominant micro/nanofluidics for efficient green energy conversion Biomicrofluidics (IF 3.2) Pub Date : 2024-02-16 Cong Wang, Eunseok Seo, Jungyul Park
Green energy conversion in aqueous systems has attracted considerable interest owing to the sustainable clean energy demand resulting from population and economic growth and urbanization, as well as the significant potential energy from water resources and other regenerative sources coupled with fluids. In particular, molecular motion based on intrinsic micro/nanofluidic phenomena at the liquid–solid
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Dean vortex-enhanced blood plasma separation in self-driven spiral microchannel flow with cross-flow microfilters Biomicrofluidics (IF 3.2) Pub Date : 2024-02-11 Yudong Wang, Niladri Talukder, Bharath Babu Nunna, Eon Soo Lee
Point-of-care (POC) diagnostic devices have been developing rapidly in recent years, but they are mainly using saliva instead of blood as a test sample. A highly efficient self-separation during the self-driven flow without power systems is desired for expanding the point-of-care diagnostic devices. Microfiltration stands out as a promising technique for blood plasma separation but faces limitations
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Strain-induced self-rolled-up microtubes for multifunctional on-chip microfluidic applications Biomicrofluidics (IF 3.2) Pub Date : 2024-01-30 Apratim Khandelwal, Xiuling Li
On-chip microfluidics are characterized as miniaturized devices that can be either integrated with other components on-chip or can individually serve as a standalone lab-on-a-chip system for a variety of applications ranging from biochemical sensing to macromolecular manipulation. Heterogenous integration with various materials and form factors is, therefore, key to enhancing the performance of such
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Engineering advancements in microfluidic systems for enhanced mixing at low Reynolds numbers Biomicrofluidics (IF 3.2) Pub Date : 2024-01-29 Vamsi Vikram Gande, Prem K. R. Podupu, Bianca Berry, Nandkishor K. Nere, S. Pushpavanam, Meenesh R. Singh
Mixing within micro- and millichannels is a pivotal element across various applications, ranging from chemical synthesis to biomedical diagnostics and environmental monitoring. The inherent low Reynolds number flow in these channels often results in a parabolic velocity profile, leading to a broad residence time distribution. Achieving efficient mixing at such small scales presents unique challenges
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Enabling biomedical technologies for chronic myelogenous leukemia (CML) biomarkers detection Biomicrofluidics (IF 3.2) Pub Date : 2024-01-25 Meenakshi Pinnenti, Muhammad Ahsan Sami, Umer Hassan
Chronic myelogenous/myeloid leukemia (CML) is a type of cancer of bone marrow that arises from hematopoietic stem cells and affects millions of people worldwide. Eighty-five percent of the CML cases are diagnosed during chronic phase, most of which are detected through routine tests. Leukocytes, micro-Ribonucleic Acids, and myeloid markers are the primary biomarkers for CML diagnosis and are mainly
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Machine learning classification of cellular states based on the impedance features derived from microfluidic single-cell impedance flow cytometry Biomicrofluidics (IF 3.2) Pub Date : 2024-01-23 Jian Wei, Wenbing Gao, Xinlong Yang, Zhuotong Yu, Fei Su, Chengwu Han, Xiaoxing Xing
Mitosis is a crucial biological process where a parental cell undergoes precisely controlled functional phases and divides into two daughter cells. Some drugs can inhibit cell mitosis, for instance, the anti-cancer drugs interacting with the tumor cell proliferation and leading to mitosis arrest at a specific phase or cell death eventually. Combining machine learning with microfluidic impedance flow
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Microfluidics enabled multi-omics triple-shot mass spectrometry for cell-based therapies Biomicrofluidics (IF 3.2) Pub Date : 2024-01-23 Gianna A. Slusher, Peter A. Kottke, Austin L. Culberson, Mason A. Chilmonczyk, Andrei G. Fedorov
In recent years, cell-based therapies have transformed medical treatment. These therapies present a multitude of challenges associated with identifying the mechanism of action, developing accurate safety and potency assays, and achieving low-cost product manufacturing at scale. The complexity of the problem can be attributed to the intricate composition of the therapeutic products: living cells with
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A microfluidic cover converts a standard 96-well plate into a mass-transport-controlled immunoassay system Biomicrofluidics (IF 3.2) Pub Date : 2024-01-18 Sheng Wang, You Zhou, Zhenyu Li
96-well microtiter plates, widely used in immunoassays, face challenges such as prolonged assay time and limited sensitivity due to the lack of analyte transport control. Orbital shakers, commonly employed to facilitate mass transport, offer limited improvements and can introduce assay inconsistencies. While microfluidic devices offer performance enhancements, their complexity and incompatibility with
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Microfluidics-based patient-derived disease detection tool for deep learning-assisted precision medicine Biomicrofluidics (IF 3.2) Pub Date : 2024-01-12 Haojun Hua, Yunlan Zhou, Wei Li, Jing Zhang, Yanlin Deng, Bee Luan Khoo
Cancer spatial and temporal heterogeneity fuels resistance to therapies. To realize the routine assessment of cancer prognosis and treatment, we demonstrate the development of an Intelligent Disease Detection Tool (IDDT), a microfluidic-based tumor model integrated with deep learning-assisted algorithmic analysis. IDDT was clinically validated with liquid blood biopsy samples (n = 71) from patients
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A perspective on computer vision in biosensing Biomicrofluidics (IF 3.2) Pub Date : 2024-01-12 Li Liu, Ke Du
Computer vision has become a powerful tool in the field of biosensing, aiding in the development of innovative and precise systems for the analysis and interpretation of biological data. This interdisciplinary approach harnesses the capabilities of computer vision algorithms and techniques to extract valuable information from various biosensing applications, including medical diagnostics, environmental
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Microphysiological systems as reliable drug discovery and evaluation tools: Evolution from innovation to maturity Biomicrofluidics (IF 3.2) Pub Date : 2023-12-28 Hye-Ran Moon, Nishanth Surianarayanan, Tarun Singh, Bumsoo Han
Microphysiological systems (MPSs), also known as organ-on-chip or disease-on-chip, have recently emerged to reconstitute the in vivo cellular microenvironment of various organs and diseases on in vitro platforms. These microfluidics-based platforms are developed to provide reliable drug discovery and regulatory evaluation testbeds. Despite recent emergences and advances of various MPS platforms, their
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Vascularized liver-on-a-chip model to investigate nicotine-induced dysfunction Biomicrofluidics (IF 3.2) Pub Date : 2023-12-27 Eric Wang, Melisa J. Andrade, Quinton Smith
The development of physiologically relevant in vitro systems for simulating disease onset and progression and predicting drug metabolism holds tremendous value in reducing drug discovery time and cost. However, many of these platforms lack accuracy in replicating the tissue architecture and multicellular interactions. By leveraging three-dimensional cell culture, biomimetic soft hydrogels, and engineered
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Acoustophoresis of monodisperse oil droplets in water: Effect of symmetry breaking and non-resonance operation on oil trapping behavior Biomicrofluidics (IF 3.2) Pub Date : 2023-12-27 H. Bazyar, M. H. Kandemir, J. Peper, M. A. B. Andrade, A. L. Bernassau, K. Schroën, R. G. H. Lammertink
Acoustic manipulation of particles in microchannels has recently gained much attention. Ultrasonic standing wave (USW) separation of oil droplets or particles is an established technology for microscale applications. Acoustofluidic devices are normally operated at optimized conditions, namely, resonant frequency, to minimize power consumption. It has been recently shown that symmetry breaking is needed
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Multiplex fluorescence detection of single-cell droplet microfluidics and its application in quantifying protein expression levels Biomicrofluidics (IF 3.2) Pub Date : 2023-12-27 Guang Yang, Chiyuan Gao, Deyong Chen, Junbo Wang, Xiaoye Huo, Jian Chen
This study presented a platform of multiplex fluorescence detection of single-cell droplet microfluidics with demonstrative applications in quantifying protein expression levels. The platform of multiplex fluorescence detection mainly included optical paths adopted from conventional microscopy enabling the generation of three optical spots from three laser sources for multiple fluorescence excitation
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Synchronous oscillatory electro-inertial focusing of microparticles Biomicrofluidics (IF 3.2) Pub Date : 2023-12-12 Giridar Vishwanathan, Gabriel Juarez
Here, results are presented on the focusing of 1μm polystyrene particle suspensions using a synchronous oscillatory pressure-driven flow and oscillatory electric field in a microfluidic device. The effect of the phase difference between the oscillatory fields on the focusing position and focusing efficiency was investigated. The focusing position of negatively charged polystyrene particles could be
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Microfluidics-enabled intelligent manufacturing of metal halide perovskite nanocrystals Biomicrofluidics (IF 3.2) Pub Date : 2023-12-12 Xiaobing Tang, Fuqian Yang
Large-scale and controllable fabrication is an indispensable step for the industrialization and commercialization of halide perovskite nanocrystals, which are new-generation semiconductor materials for optoelectronic applications. Microfluidics, which provides continuous and precise synthesis, has been considered as a promising technique to fulfill this aspect. The research studies over the past decades
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Micromixing within microfluidic devices: Fundamentals, design, and fabrication Biomicrofluidics (IF 3.2) Pub Date : 2023-12-12 Shuxiang Cai, Yawen Jin, Yun Lin, Yingzheng He, Peifan Zhang, Zhixing Ge, Wenguang Yang
As one of the hot spots in the field of microfluidic chip research, micromixers have been widely used in chemistry, biology, and medicine due to their small size, fast response time, and low reagent consumption. However, at low Reynolds numbers, the fluid motion relies mainly on the diffusive motion of molecules under laminar flow conditions. The detrimental effect of laminar flow leads to difficulties
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Toward versatile digital bioanalysis Biomicrofluidics (IF 3.2) Pub Date : 2023-12-07 Jun Ando, Rikiya Watanabe
Digital bioanalysis places great emphasis on the highly sensitive and rapid detection of biomolecules at the single-molecule level. Rooted in single-molecule biophysics, this innovative approach offers numerous insights into biomolecular mechanisms with an unprecedented level of sensitivity and precision. Moreover, this method has significant potential to contribute to disease diagnostics, enabling
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Vascularized microfluidic models of major organ structures and cancerous tissues Biomicrofluidics (IF 3.2) Pub Date : 2023-12-06 Anagha Rama Varma, Parinaz Fathi
Organ-on-a-chip devices are powerful modeling systems that allow researchers to recapitulate the in vivo structures of organs as well as the physiological conditions those tissues are subject to. These devices are useful tools in modeling not only the behavior of a healthy organ but also in modeling disease pathology or the effects of specific drugs. The incorporation of fluidic flow is of great significance
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Effect of the shear rate and residence time on the lysis of AC16 human cardiomyocyte cells via surface acoustic waves Biomicrofluidics (IF 3.2) Pub Date : 2023-12-06 G. Almanza, R. M. Trujillo, D. Sanchez-Saldaña, Ø. Rosand, M. Høydal, M. Fernandino, C. A. Dorao
The efficient breakage of one cell or a concentration of cells for releasing intracellular material such as DNA, without damaging it, is the first step for several diagnostics or treatment processes. As the cell membrane is easy to bend but resistant to stretching, the exposure of the cell to a shear rate during a short period of time can be sufficient to damage the membrane and facilitate the extraction
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On the synergy of biomicrofluidic technologies and real-time 3D tracking: A perspective Biomicrofluidics (IF 3.2) Pub Date : 2023-12-04 Liu Hong, Leonardo P. Chamorro
Particle image velocimetry and particle tracking velocimetry have played pivotal roles in flow and particle characterization, owing to their non-invasive and accurate data collection methods. However, their broader application in the biomicrofluidics field is constrained by challenges, such as intensive calibration, high post-processing costs, and optical compatibility issues, especially in settings
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A method to generate perfusable physiologic-like vascular channels within a liver-on-chip model Biomicrofluidics (IF 3.2) Pub Date : 2023-12-04 E. Ferrari, E. Monti, C. Cerutti, R. Visone, P. Occhetta, L. G. Griffith, M. Rasponi
The human vasculature is essential in organs and tissues for the transport of nutrients, metabolic waste products, and the maintenance of homeostasis. The integration of vessels in in vitro organs-on-chip may, therefore, improve the similarity to the native organ microenvironment, ensuring proper physiological functions and reducing the gap between experimental research and clinical outcomes. This
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Gas transport mechanisms through gas-permeable membranes in microfluidics: A perspective Biomicrofluidics (IF 3.2) Pub Date : 2023-11-16 Sangjin Seo, Taesung Kim
Gas-permeable membranes (GPMs) and membrane-like micro-/nanostructures offer precise control over the transport of liquids, gases, and small molecules on microchips, which has led to the possibility of diverse applications, such as gas sensors, solution concentrators, and mixture separators. With the escalating demand for GPMs in microfluidics, this Perspective article aims to comprehensively categorize
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Recent advances in droplet sequential monitoring methods for droplet sorting Biomicrofluidics (IF 3.2) Pub Date : 2023-11-13 Yukun He, Yi Qiao, Lu Ding, Tianguang Cheng, Jing Tu
Droplet microfluidics is an attractive technology to run parallel experiments with high throughput and scalability while maintaining the heterogeneous features of individual samples or reactions. Droplet sorting is utilized to collect the desired droplets based on droplet characterization and in-droplet content evaluation. A proper monitoring method is critical in this process, which governs the accuracy
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Nano/microfluidic device for high-throughput passive trapping of nanoparticles Biomicrofluidics (IF 3.2) Pub Date : 2023-11-01 Tanner Wells, Holger Schmidt, Aaron Hawkins
We present a design and a fabrication method for devices designed for rapid collection of nanoparticles in a fluid. The design uses nanofluidic channels as a passive size-based barrier trap to isolate particles near a central point in the channel, which is also covered by a thin membrane. Particles that enter the collection region are trapped with 100% efficiency within a 6–12 μm radius from a central
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Automated design of a 3D passive microfluidic particle sorter Biomicrofluidics (IF 3.2) Pub Date : 2023-11-01 Kuan-Ming Lai, Zhenya Liu, Yidan Zhang, Junchao Wang, Tsung-Yi Ho
Microfluidic chips that can sort mixtures of cells and other particles have important applications in research and healthcare. However, designing a sorter chip for a given application is a slow and difficult process, especially when we extend the design space from 2D into a 3D scenario. Compared to the 2D scenario, we need to explore more geometries to derive the appropriate design due to the extra
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Organoid-on-a-chip: Current challenges, trends, and future scope toward medicine Biomicrofluidics (IF 3.2) Pub Date : 2023-10-27 Zhangjie Li, Qinyu Li, Chenyang Zhou, Kangyi Lu, Yijun Liu, Lian Xuan, Xiaolin Wang
In vitro organoid models, typically defined as 3D multicellular aggregates, have been extensively used as a promising tool in drug screening, disease progression research, and precision medicine. Combined with advanced microfluidics technique, organoid-on-a-chip can flexibly replicate in vivo organs within the biomimetic physiological microenvironment by accurately regulating different parameters,
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Machine learning implementation strategy in imaging and impedance flow cytometry Biomicrofluidics (IF 3.2) Pub Date : 2023-10-27 Trisna Julian, Tao Tang, Yoichiroh Hosokawa, Yaxiaer Yalikun
Imaging and impedance flow cytometry is a label-free technique that has shown promise as a potential replacement for standard flow cytometry. This is due to its ability to provide rich information and archive high-throughput analysis. Recently, significant efforts have been made to leverage machine learning for processing the abundant data generated by those techniques, enabling rapid and accurate
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A review on inertial microfluidic fabrication methods Biomicrofluidics (IF 3.2) Pub Date : 2023-10-19 Zohreh Akbari, Mohammad Amin Raoufi, Sheyda Mirjalali, Behrouz Aghajanloo
In recent decades, there has been significant interest in inertial microfluidics due to its high throughput, ease of fabrication, and no need for external forces. The focusing efficiency of inertial microfluidic systems relies entirely on the geometrical features of microchannels because hydrodynamic forces (inertial lift forces and Dean drag forces) are the main driving forces in inertial microfluidic
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Diamond quantum sensors in microfluidics technology Biomicrofluidics (IF 3.2) Pub Date : 2023-10-16 Masazumi Fujiwara
Diamond quantum sensing is an emerging technology for probing multiple physico-chemical parameters in the nano- to micro-scale dimensions within diverse chemical and biological contexts. Integrating these sensors into microfluidic devices enables the precise quantification and analysis of small sample volumes in microscale channels. In this Perspective, we present recent advancements in the integration
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Blood component separation in straight microfluidic channels Biomicrofluidics (IF 3.2) Pub Date : 2023-10-16 Lap Man Lee, Ketan H. Bhatt, Dustin W. Haithcock, Balabhaskar Prabhakarpandian
Separation of blood components is required in many diagnostic applications and blood processes. In laboratories, blood is usually fractionated by manual operation involving a bulk centrifugation equipment, which significantly increases logistic burden. Blood sample processing in the field and resource-limited settings cannot be readily implemented without the use of microfluidic technology. In this
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All polymer microfluidic chips—A fixed target sample delivery workhorse for serial crystallography Biomicrofluidics (IF 3.2) Pub Date : 2023-10-13 Kevin K. Gu, Zhongrui Liu, Sankar Raju Narayanasamy, Megan L. Shelby, Nicholas Chan, Matthew A. Coleman, Matthias Frank, Tonya L. Kuhl
The development of x-ray free electron laser (XFEL) light sources and serial crystallography methodologies has led to a revolution in protein crystallography, enabling the determination of previously unobtainable protein structures and near-atomic resolution of otherwise poorly diffracting protein crystals. However, to utilize XFEL sources efficiently demands the continuous, rapid delivery of a large
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Efficient single-cell oxygen consumption rate characterization based on frequency domain fluorescence lifetime imaging microscopy measurement and microfluidic platform Biomicrofluidics (IF 3.2) Pub Date : 2023-10-13 Santhosh Kannan, Ping-Liang Ko, Hsiao-Mei Wu, Yi-Chung Tung
Cell metabolism is critical in regulating normal cell functions to maintain energy homeostasis. In order to monitor cell metabolism, the oxygen consumption rate (OCR) of cells has been characterized as an important factor. In conventional cell analysis, the cells are characterized in bulk due to technical limitations. However, the heterogeneity between the cells cannot be identified. Therefore, single-cell
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Development of a dual-flow tissue perfusion device for modeling the gastrointestinal tract–brain axis Biomicrofluidics (IF 3.2) Pub Date : 2023-10-11 Lydia Baldwin, Emily J. Jones, Alexander Iles, Simon R. Carding, Nicole Pamme, Charlotte E. Dyer, John Greenman
Despite the large number of microfluidic devices that have been described over the past decade for the study of tissues and organs, few have become widely adopted. There are many reasons for this lack of adoption, primarily that devices are constructed for a single purpose or because they are highly complex and require relatively expensive investment in facilities and training. Here, we describe a
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Enhancing single-cell biology through advanced AI-powered microfluidics Biomicrofluidics (IF 3.2) Pub Date : 2023-10-03 Zhaolong Gao, Yiwei Li
Microfluidic technology has largely benefited both fundamental biological research and translational clinical diagnosis with its advantages in high-throughput, single-cell resolution, high integrity, and wide-accessibility. Despite the merits we obtained from microfluidics in the last two decades, the current requirement of intelligence in biomedicine urges the microfluidic technology to process biological
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Bio-inspired microfluidics: A review Biomicrofluidics (IF 3.2) Pub Date : 2023-09-27 Kiran Raj M, Jyotsana Priyadarshani, Pratyaksh Karan, Saumyadwip Bandyopadhyay, Soumya Bhattacharya, Suman Chakraborty
Biomicrofluidics, a subdomain of microfluidics, has been inspired by several ideas from nature. However, while the basic inspiration for the same may be drawn from the living world, the translation of all relevant essential functionalities to an artificially engineered framework does not remain trivial. Here, we review the recent progress in bio-inspired microfluidic systems via harnessing the integration
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A facile fluid pressure system reveals differential cellular response to interstitial pressure gradients and flow Biomicrofluidics (IF 3.2) Pub Date : 2023-09-27 Hao Wang, Jingming Lu, Mitesh Rathod, Wen Yih Aw, Stephanie A. Huang, William J. Polacheck
Interstitial fluid pressure gradients and interstitial flow have been shown to drive morphogenic processes that shape tissues and influence progression of diseases including cancer. The advent of porous media microfluidic approaches has enabled investigation of the cellular response to interstitial flow, but questions remain as to the critical biophysical and biochemical signals imparted by interstitial
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Label-free microfluidic isolation of functional and viable lymphocytes from peripheral blood mononuclear cells Biomicrofluidics (IF 3.2) Pub Date : 2023-09-19 Abhishek Raj, Katily Ramirez, Katherine M. Young, Nicholas Stone, Peter Shankles, Mehdia Nadeem Rajab Ali, Anthony Malik Compton, Wilbur Lam, Alexander Alexeev, Todd Sulchek
The separation of peripheral blood mononuclear cells (PBMCs) into constituent blood cell types is a vital step to obtain immune cells for autologous cell therapies. The ability to separate PBMCs using label-free microfluidic techniques, based on differences in biomechanical properties, can have a number of benefits over other conventional techniques, including lower cost, ease of use, and avoidance
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Microfluidic pumps for cell sorting Biomicrofluidics (IF 3.2) Pub Date : 2023-09-19 Leyla Akh, Diane Jung, William Frantz, Corrin Bowman, Anika C. Neu, Xiaoyun Ding
Microfluidic cell sorting has shown promising advantages over traditional bulky cell sorting equipment and has demonstrated wide-reaching applications in biological research and medical diagnostics. The most important characteristics of a microfluidic cell sorter are its throughput, ease of use, and integration of peripheral equipment onto the chip itself. In this review, we discuss the six most common
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Protein array processing software for automated semiquantitative analysis of serum antibody repertoires Biomicrofluidics (IF 3.2) Pub Date : 2023-09-15 Ajeet Singh Yadav, Chin Hong Ooi, Hongjie An, Nam-Trung Nguyen, Gregor S. Kijanka
Effective immunotherapies activate natural antitumor immune responses in patients undergoing treatment. The ability to monitor immune activation in response to immunotherapy is critical in measuring treatment efficacy over time and across patient cohorts. Protein arrays are systematically arranged, large collections of annotated proteins on planar surfaces, which can be used for the characterization
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Erratum: "Microfluidic-to-macrofluidic: A simple in vitro model of atherosclerosis induced by fluidic stimulation" [Biomicrofluidics 17, 044106 (2023)]. Biomicrofluidics (IF 3.2) Pub Date : 2023-09-13 Jingang Cui,Wei Jiang,Jilei Su,Jiazhen Zhang,Yongliang Yu,Yongsheng Ding
[This corrects the article DOI: 10.1063/5.0155267.].
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Microfluidic-to-macrofluidic: A simple in vitro model of atherosclerosis induced by fluidic stimulation Biomicrofluidics (IF 3.2) Pub Date : 2023-08-29 Jingang Cui, Wei Jiang, Jilei Su, Jiazhen Zhang, Yongliang Yu, Yongsheng Ding
Atherosclerosis is the narrowing of the arteries due to the formation of fatty plaques, which is the main cause of myocardial infarction and stroke. It is important to develop an in vitro model that can combine multiple-type cell co-culture, vessel wall-like structure, and fluid condition to simulate the processes of atherosclerosis. Herein, we used a simple microfluidic chip made of three polydimethylsiloxane
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A high throughput blood–brain barrier model incorporating shear stress with improved predictive power for drug discovery Biomicrofluidics (IF 3.2) Pub Date : 2023-08-21 Daniel Chavarria, Ali Abbaspour, Natalie Celestino, Nehali Shah, Sharanya Sankar, Aaron B. Baker
The blood–brain barrier is a key structure regulating the health of the brain and access of drugs and pathogens to neural tissue. Shear stress is a key regulator of the blood–brain barrier; however, the commonly used multi-well vitro models of the blood–brain barrier do not incorporate shear stress. In this work, we designed and validated a high-throughput system for simulating the blood–brain barrier
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Artificial intelligence-driven electrochemical immunosensing biochips in multi-component detection Biomicrofluidics (IF 3.2) Pub Date : 2023-08-21 Yuliang Zhao, Xiaoai Wang, Tingting Sun, Peng Shan, Zhikun Zhan, Zhongpeng Zhao, Yongqiang Jiang, Mingyue Qu, Qingyu Lv, Ying Wang, Peng Liu, Shaolong Chen
Electrochemical Immunosensing (EI) combines electrochemical analysis and immunology principles and is characterized by its simplicity, rapid detection, high sensitivity, and specificity. EI has become an important approach in various fields, such as clinical diagnosis, disease prevention and treatment, environmental monitoring, and food safety. However, EI multi-component detection still faces two
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Continuous separation of bacterial cells from large debris using a spiral microfluidic device Biomicrofluidics (IF 3.2) Pub Date : 2023-08-09 Ayomikun Esan, Frédérique Vanholsbeeck, Simon Swift, Cushla M. McGoverin
With the global increase in food exchange, rapid identification and enumeration of bacteria has become crucial for protecting consumers from bacterial contamination. Efficient analysis requires the separation of target particles (e.g., bacterial cells) from food and/or sampling matrices to prevent matrix interference with the detection and analysis of target cells. However, studies on the separation
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Solvent-pumped evaporation concentration on paper in linear and radial geometries Biomicrofluidics (IF 3.2) Pub Date : 2023-08-09 Richard R. A. Syms, Steven Wright
Solvent-pumped evaporation-driven concentration of an initial distribution of solutes on a porous substrate is considered in one and two dimensions. Approximate analytic solutions to the isotropic advection–dispersion equations are first found for a Gaussian kernel and an infinite domain, following the smoothed particle approximation. Analytic solutions for more general initial distributions are then
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Photonic crystal enhanced immunofluorescence biosensor integrated with a lateral flow microchip: Toward rapid tear-based diabetic retinopathy screening Biomicrofluidics (IF 3.2) Pub Date : 2023-07-20 Li-Ying Chen, Sheng-Min Hsu, Jhih-Cheng Wang, Tai-Hua Yang, Han-Sheng Chuang
Diabetic retinopathy (DR) has accounted for major loss of vision in chronic diabetes. Although clinical statistics have shown that early screening can procrastinate or improve the deterioration of the disease, the screening rate remains low worldwide because of the great inconvenience of conventional ophthalmoscopic examination. Instead, tear fluid that contains rich proteins caused by direct contact
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Machine learning-augmented fluid dynamics simulations for micromixer educational module Biomicrofluidics (IF 3.2) Pub Date : 2023-07-06 Mehmet Tugrul Birtek, M. Munzer Alseed, Misagh Rezapour Sarabi, Abdollah Ahmadpour, Ali K. Yetisen, Savas Tasoglu
Micromixers play an imperative role in chemical and biomedical systems. Designing compact micromixers for laminar flows owning a low Reynolds number is more challenging than flows with higher turbulence. Machine learning models can enable the optimization of the designs and capabilities of microfluidic systems by receiving input from a training library and producing algorithms that can predict the
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Microfluidics based bioimaging with cost-efficient fabrication of multi-level micrometer-sized trenches Biomicrofluidics (IF 3.2) Pub Date : 2023-06-15 Anand Anilkumar, Abhilasha Batra, Santanu Talukder, Rati Sharma
Microfluidic devices, through their vast applicability as tools for miniaturized experimental setups, have become indispensable for cutting edge research and diagnostics. However, the high operational cost and the requirement of sophisticated equipment and clean room facility for the fabrication of these devices make their use unfeasible for many research laboratories in resource limited settings.
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Lateral force separation of biopolymers using an atomic force microscope Biomicrofluidics (IF 3.2) Pub Date : 2023-05-25 Mark S. Anderson
The lateral force separation of long chain biomolecules is demonstrated using an atomic force microscope (AFM). This is achieved by using an AFM tip to pull molecules away from the edge of a nanofluidic solution. By monitoring the torsion on the AFM cantilever, a characteristic force–distance signal is produced when long chain molecules separate and detach from the solvent edge. This lateral force
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Wall shear gradient dependent thrombosis studied in blood-on-a-chip with stenotic, branched, and valvular constructions Biomicrofluidics (IF 3.2) Pub Date : 2023-05-10 Yan Li, Yongjian Li, Jiang Li, Haosheng Chen
Thrombosis is the leading cause of death, while the effect of the shear flow on the formation of thrombus in vascular constructions has not been thoroughly understood, and one of the challenges is to observe the origination of thrombus with a controlled flow field. In this work, we use blood-on-a-chip technology to mimic the flow conditions in coronary artery stenosis, neonatal aortic arch, and deep
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Directed colloidal assembly and banding via DC electrokinetics Biomicrofluidics (IF 3.2) Pub Date : 2023-05-10 Sangwoo Shin
Manipulating the transport and assembly of colloidal particles to form segregated bands or ordered supracolloidal structures plays an important role in many aspects of science and technology, from understanding the origin of life to synthesizing new materials for next-generation manufacturing, electronics, and therapeutics. One commonly used method to direct colloidal transport and assembly is the