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  • Alterations in egg white-related genes expression in response to hormonal stimulation.
    Reproduction (IF 3.206) Pub Date : 2020-08-28
    Minkyeong Lee,Changwon Yang,Gwonhwa Song,Whasun Lim

    The reproductive tract in avian females is sensitive to hormonal regulation. Exogenous estrogen induces immature oviduct development to improve egg production after molting. In this process, regressed female reproductive tract is regenerated in response to the secretion of estrogen. However, there is limited knowledge on the physiological mechanisms underlying the regulation of the avian female reproductive

    更新日期:2020-08-29
  • ANEUPLOIDY DURING THE ONSET OF MOUSE EMBRYO DEVELOPMENT
    Reproduction (IF 3.206) Pub Date : 2020-08-28
    Tereza Pauerova; Lenka Radonova; Kristina Kovacovicova; Lucia Novakova; Michal Skultety; Martin Anger

    ABSTRACT: Aneuploidy is the most frequent single cause leading into termination of early development in human and animal reproduction. Although mouse is frequently used as model organism for studying the aneuploidy, we have only incomplete information about the frequency of numerical chromosomal aberrations throughout development, usually limited to a particular stage or assumed from the occurrence

    更新日期:2020-08-29
  • Antisperm antibodies and sperm function in bulls undergoing scrotal insulation
    Reproduction (IF 3.206) Pub Date : 2020-08-28
    Maria S. Ferrer; Roberto Palomares; David Hurley; Anna-Claire Bullington; Alejandro Hoyos-Jaramillo; João H. Bittar

    Bovine antisperm antibodies (ASAs) have been associated with teratospermia and asthenospermia. It was hypothesized here that scrotal insulation induces formation of ASAs and deterioration of sperm function. Scrotal insulation bags were placed in 10 bulls for 8 days. Semen was collected on days -29, -22 and -2, twice weekly from days 5 to 54, and thereafter weekly until day 96 (day 0=first day of scrotal

    更新日期:2020-08-29
  • Derivation of sheep embryonic stem cells under optimized conditions
    Reproduction (IF 3.206) Pub Date : 2020-08-28
    Marcela Vilarino; Delia Alba Soto; Yanina Solsdad Bogliotti; Leqian Yu; Yanli Zhang; Chunsheng Wang; Erika Paulson; Cuiqing Zhong; Miaohan Jin; Juan Carlos Izpisua Belmonte; Jun Wu; Pablo Juan Ross

    Until recently, it has been difficult to derive and maintain stable embryonic stem cells lines from livestock species. Sheep ESCs with characteristics similar to those described for rodents and primates have not been produced. We report the derivation of sheep ESCs under a chemically defined culture system containing fibroblast growth factor 2 (FGF2) and a tankyrase/Wnt inhibitor (IWR1). We also show

    更新日期:2020-08-29
  • Genetics of premature ovarian insufficiency and the association with X-autosome translocations.
    Reproduction (IF 3.206) Pub Date : 2020-08-25
    Adriana Di-Battista,Mariana Moysés-Oliveira,Maria Isabel Melaragno

    Premature ovarian insufficiency (POI) is the cessation of menstruation before the age of 40 and can result from different etiologies, including genetic, autoimmune, and iatrogenic. Of the genetic causes, single-gene mutations and cytogenetic alterations, such as X-chromosome aneuploidies and chromosome rearrangements, can be associated with POI. In this review, we summarize the genetic factors linked

    更新日期:2020-08-25
  • Inflammation, but not infection, induces EMT in human amnion epithelial cells.
    Reproduction (IF 3.206) Pub Date : 2020-08-25
    Mariana de Castro Silva,Lauren S Richardson,Talar Kechichian,Rheanna Urrabaz-Garza,Márcia Guimarães da Silva,Ramkumar Menon

    A non-reversible state of epithelial to mesenchymal transition (EMT) at term accumulates proinflammatory mesenchymal cells and predisposes fetal membrane to weakening prior to delivery at term. We investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and infection associated with spontaneous preterm birth (SPTB). For this, membranes from SPTB were screened for

    更新日期:2020-08-25
  • Canine oviductal exosomes improve oocyte development via EGFR/MAPK signaling pathway.
    Reproduction (IF 3.206) Pub Date : 2020-08-24
    Seok Hee Lee,Hyun Ju Oh,Min Jung Kim,Byeong Chun Lee

    Oviduct cells produce a favorable environment for the development of gametes by generating multiple growth factors. Particularly, in canine species, immature oocytes undergo serial maturation processes in the oviduct, while the other mammals already possess matured oocytes in ovulatory follicles. However, little is known about the potential effect exhibited by the components released from canine oviduct

    更新日期:2020-08-24
  • 30 years of IVF and Preimplantation Genetic Testing
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Alan H. Handyside

    ?Not applicable

    更新日期:2020-08-20
  • CHRONODISRUPTION: EFFECTS ON REPRODUCTION, TRANSGENERATIONAL HEALTH OF OFFSPRING AND EPIGENOME
    Reproduction (IF 3.206) Pub Date : 2020-08-13
    Leyla Sati

    The circadian system regulates the daily temporal organization in behavior and physiology, including neuroendocrine rhythms and reproduction. Modern life, however, increasingly impacts this complex biological system. Due to limitations of working with human subjects exposed to shiftwork schedules, most chronoregulation research has used rodent models. Recent publications in these model systems have

    更新日期:2020-08-20
  • Effect of aflatoxin B1 on bovine spermatozoa's proteome and embryo's transcriptome
    Reproduction (IF 3.206) Pub Date : 2020-08-07
    Alisa Komsky-Elbaz; Dorit Kalo; Zvi Roth

    This study aims to evaluate the deleterious effect of the mycotoxin aflatoxin B1 (AFB1) on bull spermatozoa and the carry-over effect on the developing embryo. Proteomic analysis of AFB1-treated spermatozoa revealed differential expression of proteins associated with biological processes and cellular pathways that involved in spermatozoon function, fertilization competence and embryonic development

    更新日期:2020-08-20
  • Equine endometritis: A review of challenges and new approaches.
    Reproduction (IF 3.206) Pub Date : 2020-08-14
    Lee Ha Morris,Patrick M McCue,Christine Aurich

    Endometritis in the mare begins as a normal physiological inflammatory response to breeding that involves both a mechanical and immunological response pathway activated to rid the uterus of semen and bacteria. With successful resolution of this inflammation, the mare’s uterus will provide a hospitable environment for the development of the semi-allogenic conceptus. If the mare fails to resolve this

    更新日期:2020-08-20
  • Equine pregnancy-specific glycoprotein CEACAM49 secreted by endometrial cup cells activates TGFB
    Reproduction (IF 3.206) Pub Date : 2020-08-07
    Robert Kammerer; Angela Ballesteros; Daniel Bonsor; James Warren; John M Williams; Tom Moore; Gabriela Dveksler

    In early equine pregnancy a highly invasive trophoblast cell subpopulation, the chorionic girdle cells, invade the endometrium and form endometrial cups (EC). These cells express classical MHC molecules, thereby stimulating a humoral and cellular immune response, resulting in a massive accumulation of maternal CD4+ and CD8+ T cells around the EC. Nevertheless, no immediate destruction of endometrial

    更新日期:2020-08-20
  • Human endometrial stromal cells inhibit the pro-angiogenic stimulus of hCG in vitro
    Reproduction (IF 3.206) Pub Date : 2020-08-07
    Marcia Riboldi; Ivonne Nazir; Belen Jara; Felipe Argandoña; Cecilia Valencia; Paulo C. Serafini; Eduardo Leme Alves Motta; Dennise Mena-Silva; Reinaldo González; M Cecilia Johnson; Ariel Fuentes; Karina Sequeira; Alejandro Tapia-Pizarro

    During embryo implantation, endometrial angiogenesis is regulated by signals originating from the endometrium itself and the developing embryo. It has been suggested that hCG may play a pro-angiogenic role; therefore, we sought to understand its regulatory role in blood vessel formation in human endometrium using in vivo and in vitro models. In the in vivo model, we screened 16 angiogenesis-related

    更新日期:2020-08-20
  • Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa.
    Reproduction (IF 3.206) Pub Date : 2020-08-17
    Roisin Ann Griffin,Aleona Swegen,Mark Baker,Robert John Aitken,David A Skerrett-Byrne,Antonio Silva Rodriguez,Francisco E Martín-Cano,Brett Nixon,Fernando J Pena,Maryse Delehedde,Nicolas Sergeant,Zamira Gibb

    The horse breeding industry relies upon optimal stallion fertility. Conventional sperm assessments provide limited information regarding ejaculate quality and are not individually predictive of fertilising potential. The aim of this study was to harness mass spectrometry to compare the proteomic profiles of high- and low-quality stallion spermatozoa, with the ultimate goal of identifying fertility

    更新日期:2020-08-20
  • Maternal Iron Homeostasis: Effect on Placental Development and Function
    Reproduction (IF 3.206) Pub Date : 2020-08-07
    Hannah Roberts; Stephane L. Bourque; Stephen J Renaud

    Iron is an essential mineral that participates in oxygen transport, DNA synthesis and repair, and as a cofactor for various cellular processes. Iron deficiency is the most common nutritional deficiency worldwide. Due to blood volume expansion and demands from the fetal-placental unit, pregnant women are one of the populations most at risk of developing iron deficiency. Iron deficiency during pregnancy

    更新日期:2020-08-20
  • Metformin Attenuates Steroidogenesis in Ovarian Follicles of the Broiler Breeder Hen
    Reproduction (IF 3.206) Pub Date : 2020-08-03
    Evelyn A Weaver; Ramesh Ramachandran

    The follicular hierarchy in broiler breeder chicken ovary is often deranged due to excessive ovarian follicular recruitment, resulting in a condition that resembles polycystic ovary syndrome (PCOS) in women. Metformin is widely prescribed to correct PCOS and has been shown to affect granulosa cell functions in humans and rodent models. The objectives of this study are to determine the effects of metformin

    更新日期:2020-08-20
  • Obesity alters ovarian DNA damage response and apoptotic proteins
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Kendra L. Clark; Crystal M. Roach; Aileen F. Keating

    In the overweight or obese female, reproductive complications include poor oocyte quality, decreased fecundity, gestational diabetes, and higher risk of reproductive cancers. Using lean and hyperphagia-induced obese female mice aged 10 weeks, we determined that the ovary from obese female mice had elevated (P < 0.10) levels of ataxia telangiectasia mutated (ATM) protein in oocytes of both small and

    更新日期:2020-08-20
  • Physical activity throughout pregnancy is key to preventing chronic disease.
    Reproduction (IF 3.206) Pub Date : 2020-08-17
    Taniya S Nagpal,Michelle F Mottola

    According to The Developmental Origins of Health and Disease theory, the intra-uterine environment of the developing fetus may impact later life physiology, including susceptibility to chronic disease conditions. Maternal exposures during pregnancy can affect the intra-uterine environment and result in fetal programming for chronic diseases through changes in the structure or function of specific organs

    更新日期:2020-08-20
  • The presence and role of hypoxia in the endometrium
    Reproduction (IF 3.206) Pub Date : 2020-08-03
    Rocío Martínez-Aguilar; Lucy E Kershaw; Jane J Reavey; Hilary O D Critchley; Jacqueline A Maybin

    The endometrium is a multicellular tissue that is exquisitely responsive to the ovarian hormones. The local mechanisms of endometrial regulation to ensure optimal function are less well characterised. Transient physiological hypoxia has been proposed as a critical regulator of endometrial function. Herein, we review the literature on hypoxia in the non-pregnant endometrium. We discuss the pros and

    更新日期:2020-08-20
  • Pro-angiogenic effects of pregnancy-specific glycoproteins in endothelial and extravillous trophoblast cells
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Shemona Rattila; Florian Kleefeldt; Angela Ballesteros; Jimena S. Beltrem; María L. Ribeiro; Suleyman Ergun; Gabriela Dveksler

    We previously reported that binding to heparan sulfate (HS) is required for the ability of the placentally-secreted pregnancy-specific glycoprotein 1 (PSG1) to induce endothelial tubulogenesis. PSG1 is composed of four immunoglobulin-like domains but which domains of the protein bind to HS remains unknown. To analyze the interaction of PSG1 with HS, we generated several recombinant proteins, including

    更新日期:2020-08-20
  • PROTEOMICS AND METABOLOMICS IN COW FERTILITY: A SYSTEMATIC REVIEW
    Reproduction (IF 3.206) Pub Date : 2020-08-04
    Nicolas Aranciaga; James D Morton; Debra K Berg; Jessica L Gathercole

    Cow subfertility is a multi-factorial problem in many countries which is only starting to be unravelled. Molecular biology can provide a substantial source of insight into its causes and potential solutions, particularly through large scale, untargeted omics approaches. In this systematic review we set out to compile, assess and integrate the latest proteomic and metabolomic research on cow reproduction

    更新日期:2020-08-20
  • Single-cell technologies at the forefront of PGT and embryo research
    Reproduction (IF 3.206) Pub Date : 2020-08-13
    Olga Tsuiko; Elia Fernandez Gallardo; Thierry Voet; Joris Robert Vermeesch

    While chromosomal mosaicism in the embryo was observed already in the ‘90s using both karyotyping and FISH technologies, the full extent of this phenomenon and the overall awareness of the consequences of chromosomal instability on embryo development has only come with the advent of sophisticated single-cell technologies. High-throughput techniques, such as DNA microarrays and massive parallel sequencing

    更新日期:2020-08-20
  • Sperm binding to ZP2-coated beads improve efficiency of porcine in vitro fertilization
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Julieta Gabriela Hamze; María Jiménez-Movilla; Raquel Romar

    The role of specific zona pellucida (ZP) glycoproteins in gamete interaction has not yet been elucidated in many species. A recently developed 3D model based on magnetic sepharose beads (B) conjugated to recombinant ZP glycoproteins (BZP) and cumulus cells (CBZP) allows the study of isolated ZP proteins in gamete recognition studies. The objective of this work was to study the role of porcine ZP2,

    更新日期:2020-08-20
  • Nucleolar stress regulates stromal-epithelial transition via NPM1 during decidualization.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Yu-Xiang Liang,Wei Hu,Zhi-Yong Jin,Hong-Lu Diao,Li Liu,Yan Yang,Tao Fu,Zeng-Ming Yang

    Embryo implantation and decidualization are crucial steps during early pregnancy. We recently showed that nucleolar stress is involved in embryo implantation. This study was to explore whether nucleolar stress participates in mouse and human decidualization. Our data demonstrated that a low dose of actinomycin D (ActD) could induce nucleolar stress in stroma cells. Nucleolar stress promotes the stromal-epithelial

    更新日期:2020-08-20
  • Milk exosomes and miRNA cross the placenta and promote embryo survival in mice.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Mahrou Sadri,Jiang Shu,Stephen D Kachman,Juan Cui,Janos Zempleni

    Exosomes facilitate cell-to-cell communication by transferring regulatory molecules such as miRNA from donor to recipient cells, for example, miR-21-5p and miR-30d promote placentation. Exosomes and their miRNA cargos are not exclusively obtained from endogenous synthesis but may also be absorbed from dietary sources, such as milk. This study assessed the effects of milk exosomes and miRNA cargos on

    更新日期:2020-08-20
  • Estrogen-decreased hsa_circ_0001649 promotes stromal cell invasion in endometriosis.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Qi Li,Na Li,Hengwei Liu,Yu Du,Haitang He,Ling Zhang,Yi Liu

    Endometriosis (EMs) is an estrogen (E2)-dependent inflammatory disorder. Although EMs is considered a benign disease, it presents with malignant characteristics, such as migration and invasion. An increasing number of studies have shown that aberrantly expressed circular RNAs (circRNAs) play an essential role in disease development and progression. However, the mechanisms by which circRNAs exert their

    更新日期:2020-08-20
  • Integrins and OPN localize to adhesion complexes during placentation in sheep.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Heewon Seo,James W Frank,Robert C Burghardt,Fuller W Bazer,Greg A Johnson

    Integrins and OPN are potential mediators of blastocyst attachment to the endometrium to initiate implantation. The goals were to examine the temporal/spatial pattern of expression of integrins at the endometrial–placental interface of sheep encompassing Days 9 through 80 of gestation and determine if OPN co-localizes with integrins. Results show the following: (1) αv, α4, β1, β3 and β5 integrins at

    更新日期:2020-08-20
  • Membrane trafficking directed by VAMP2 and syntaxin 3 in uterine epithelial cells.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Sadaf N Kalam,Louise Cole,Laura Lindsay,Christopher R Murphy

    Luminal uterine epithelial cells (UEC) have a surge in vesicular activity during early uterine receptivity. It has been predicted these vesicles exit the UEC via exocytosis resulting in secretion and membrane trafficking. The present study investigated the changes in SNARE proteins VAMP2 (v-SNARE) and syntaxin 3 (t-SNARE) localisation and abundance in UECs during early pregnancy in the rat. We found

    更新日期:2020-08-20
  • Pluripotent stem cells are insensitive to the cytotoxicity of TNFα and IFNγ.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Bohan Chen,Chandan Gurung,Jason Guo,Chulan Kwon,Yan-Lin Guo

    Recent studies have demonstrated that embryonic stem cells (ESCs) have an underdeveloped innate immune system, but the biological implications of this finding are poorly understood. In this study, we compared the responses of mouse ESCs (mESCs) and mESC differentiated fibroblasts (mESC-FBs) to tumor necrosis factor α (TNFα) and interferons (IFNs). Our data revealed that TNFα, IFNα, IFNβ, or IFNγ alone

    更新日期:2020-08-20
  • Anti-inflammatory effects of gallic acid in human gestational tissues in vitro.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Caitlyn Nguyen-Ngo,Carlos Salomon,Andrew Lai,Jane C Willcox,Martha Lappas

    Spontaneous preterm birth is the leading cause of neonatal mortality and morbidity globally. Activation of the maternal immune system leads to a downstream cascade of proinflammatory events that culminate in the activation of spontaneous uterine contractions and the rupture of the foetal membranes. Anti-inflammatory agents may be a novel therapeutic approach to prevent inflammation-induced myometrial

    更新日期:2020-08-20
  • Embryonic disc formation following post-hatching bovine embryo development in vitro.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Priscila Ramos-Ibeas,Ismael Lamas-Toranzo,Álvaro Martínez-Moro,Celia de Frutos,Alejandra C Quiroga,Esther Zurita,Pablo Bermejo-Álvarez

    Failures during conceptus elongation are a major cause of pregnancy losses in ungulates, exerting a relevant economic impact on farming. The developmental events occurring during this period are poorly understood, mainly because this process cannot be recapitulated in vitro. Previous studies have established an in vitro post-hatching development (PHD) system that supports bovine embryo development

    更新日期:2020-08-20
  • Crosstalk between estradiol and NFκB signaling pathways on placental leptin expression.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Malena Schanton,Julieta Maymó,María Fernanda Camisay,Antonio Pérez-Pérez,Roberto Casale,Víctor Sánchez-Margalet,Alejandra Erlejman,Cecilia Varone

    Pregnancy success requires a proper fetal maternal interaction at the establishment of implantation. Leptin has been described as a multitasking cytokine in pregnancy, particularly in the placenta, where it acts as an autocrine hormone. The expression of leptin in normal trophoblastic cells is regulated by different endogenous signals. We have previously reported that 17β-estradiol upregulates placental

    更新日期:2020-08-20
  • Pentraxin-3 mediates prosurvival actions of interferon tau in bovine luteinized granulosa cells.
    Reproduction (IF 3.206) Pub Date : 2020-08-20
    Raghavendra Basavaraja,Senasige Thilina Madusanka,Ketan Shrestha,Emilia Przygrodzka,Monika Marzena Kaczmarek,Rina Meidan

    Pentraxin 3 (PTX3), a multimeric glycoprotein, is implicated in various biological functions. PTX3 was shown to be elevated in the corpus luteum (CL) of early pregnant ewes; however, its role in sheep or other ruminants’ CL during this reproductive stage or how it is regulated remain unknown. Here we explored the role of PTX3 and its relationship with interferon-tau (IFNT; the pregnancy recognition

    更新日期:2020-08-20
  • Association of placental nutrient sensing pathways with birth weight.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Maria-Luisa Lazo-de-la-Vega-Monroy,Karen-Alejandra Mata-Tapia,Juan-Antonio Garcia-Santillan,Maria-Angelica Corona-Figueroa,Martha-Isabel Gonzalez-Dominguez,Hector-Manuel Gomez-Zapata,Juan-Manuel Malacara,Leonel Daza-Benitez,Gloria Barbosa-Sabanero

    Birth weight (BW) is an important indicator for newborn health. Both high and low BW is associated with increased risks for adult metabolic diseases. AMPK (AMP-activated protein kinase), mTOR (mechanistic target of rapamycin), and insulin/IGF1 (insulin-like growth factor 1) pathways may function as placental sensors of maternal hormonal and nutritional status. However, the physiological role of these

    更新日期:2020-08-14
  • Nesfatin-1 suppresses fish reproductive axis and gonadal steroidogenesis.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Jithine Jayakumar Rajeswari,Suraj Unniappan

    Nesfatin-1 is a naturally occurring orphan ligand in fish and mammals. Research in our lab resulted in the identification of an inhibitory role for nesfatin-1 on pituitary hormones (goldfish) and oocyte maturation (zebrafish). The present study is an extension of these original findings and aimed to determine whether nesfatin-1 has any additional effects on HPG genes in male and female goldfish. We

    更新日期:2020-08-14
  • Cyclophilin A plays an important role in embryo implantation through activating Stat3.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Tao Yu,Shuai Lin,Rui Xu,Tian-Xi Du,Yang Li,Hui Gao,Hong-Lu Diao,Xiu-Hong Zhang

    Embryo implantation is a crucial step for the successful establishment of mammalian pregnancy. Cyclophilin A (CYPA) is a ubiquitously expressed intracellular protein and is secreted in response to inflammatory stimuli to regulate diverse cellular functions. However, there are currently no reports about the role of CYPA in embryo implantation. Here, we examine the expression pattern of CYPA during mouse

    更新日期:2020-08-14
  • Validity of the association between periodontitis and female infertility conditions: a concise review.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Vanessa Machado,Joana Lopes,Mariana Patrão,João Botelho,Luís Proença,José João Mendes

    Hormones and inflammatory mechanisms are implicated with female reproductive function, including follicle maturation, ovulation, embryo implantation, and pregnancy. Periodontitis is a chronic inflammatory disease due to a polymicrobial disruption of the homeostasis and may be considered as a potential risk factor that affect female fertility. The role of periodontitis is becoming meaningful, with significant

    更新日期:2020-08-14
  • The ovarian stroma as a new frontier.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Hadrian M Kinnear,Claire E Tomaszewski,Faith L Chang,Molly B Moravek,Min Xu,Vasantha Padmanabhan,Ariella Shikanov

    Historically, research in ovarian biology has focused on folliculogenesis, but recently the ovarian stroma has become an exciting new frontier for research, holding critical keys to understanding complex ovarian dynamics. Ovarian follicles, which are the functional units of the ovary, comprise the ovarian parenchyma, while the ovarian stroma thus refers to the inverse or the components of the ovary

    更新日期:2020-08-14
  • PDIA3 regulates trophoblast apoptosis and proliferation in preeclampsia via the MDM2/p53 pathway.
    Reproduction (IF 3.206) Pub Date : 2020-08-01
    Hui-Qin Mo,Fu-Ju Tian,Xiao-Ling Ma,Yu-Chen Zhang,Cheng-Xi Zhang,Wei-Hong Zeng,Yan Zhang,Yi Lin

    Protein disulfide isomerase 3 (PDIA3) is a chaperone protein that modulates the folding of newly synthesized glycoproteins, has isomerase and redox activity, and has been implicated in the pathogenesis of many diseases. However, the role of PDIA3 in pregnancy-associated diseases remains largely unknown. Our present study reveals a key role for PDIA3 in the biology of placental trophoblasts from women

    更新日期:2020-08-01
  • Birth of offspring from spermatid or somatic cell by co-injection of PLCζ-cRNA.
    Reproduction (IF 3.206) Pub Date : 2020-08-01
    Naoki Hirose,Sayaka Wakayama,Rei Inoue,Junya Ito,Masatoshi Ooga,Teruhiko Wakayama

    Artificial oocyte activation is important for assisted reproductive technologies, such as fertilization with round spermatids (ROSI) or the production of cloned offspring by somatic cell nuclear transfer (SCNT). Recently, phospholipase Cζ (PLCζ)-cRNA was used to mimic the natural process of fertilization, but this method required the serial injection of PLCζ-cRNA and was found to cause damage to the

    更新日期:2020-08-01
  • YAP and TAZ are required for the postnatal development and the maintenance of the structural integrity of the oviduct.
    Reproduction (IF 3.206) Pub Date : 2020-08-01
    Philippe Godin,Mayra Tsoi,Marilène Paquet,Derek Boerboom

    The development of the Müllerian ducts into the female reproductive tract requires the coordination of multiple signaling pathways that regulate proliferation, apoptosis and differentiation. The Hippo pathway has been reported to interact with several pathways with established roles in Müllerian duct development; yet, its potential roles in reproductive tract development and function remain mostly

    更新日期:2020-06-11
  • Hormonally controlled ILC antigen presentation potential is reduced during pregnancy.
    Reproduction (IF 3.206) Pub Date : 2020-07-01
    Rebekka Einenkel,Jens Ehrhardt,Kristin Hartmann,Diana Krüger,Damián Oscar Muzzio,Marek Zygmunt

    Strategically located in mucosal barriers, innate lymphoid cells (ILCs) are relevant in local containment and tolerance of commensal microflora. ILCs have been recently described at the fetomaternal interface, where the development of a semi-allogeneic fetus can only succeed in a well-controlled immune environment. We postulate that ILCs adapt their antigen presentation capacity to protect pregnancy

    更新日期:2020-06-09
  • Mating with immunised male mice affects the phenotype of adult progeny.
    Reproduction (IF 3.206) Pub Date : 2020-07-01
    Ludmila Gerlinskaya,Margarita Anisimova,Galina Kontsevaya,Svetlana Maslennikova,Alexander Romashchenko,Yanli Gong,Yuri Moshkin,Mikhail Moshkin

    The life-history theory suggests that parental experience of the environment is passed to offspring, which allows them to adapt to prevailing conditions. This idea is supported from the mother's side, but to a much less extent from the father's side. Here, we investigated the effect of immunising fathers on pre- and neonatal development and on immune and neuroendocrine phenotypes of their offspring

    更新日期:2020-06-09
  • Glucocorticoids impair oocyte competence and trigger apoptosis of ovarian cells via activating the TNF-α system.
    Reproduction (IF 3.206) Pub Date : 2020-07-01
    Hong-Jie Yuan,Zhi-Bin Li,Xin-Yue Zhao,Guang-Yi Sun,Guo-Liang Wang,Ying-Qi Zhao,Min Zhang,Jing-He Tan

    Mechanisms by which female stress and particularly glucocorticoids impair oocyte competence are largely unclear. Although one study demonstrated that glucocorticoids triggered apoptosis in ovarian cells and oocytes by activating the FasL/Fas system, other studies suggested that they might induce apoptosis through activating other signaling pathways as well. In this study, both in vivo and in vitro

    更新日期:2020-06-09
  • Modeling human trophoblast, the placental epithelium at the maternal fetal interface.
    Reproduction (IF 3.206) Pub Date : 2020-07-01
    Mariko Horii,Ojeni Touma,Tony Bui,Mana M Parast

    Appropriate human trophoblast lineage specification and differentiation is crucial for the establishment of normal placentation and maintenance of pregnancy. However, due to the lack of proper modeling systems, the molecular mechanisms of these processes are still largely unknown. Much of the early studies in this area have been based on animal models and tumor-derived trophoblast cell lines, both

    更新日期:2020-06-09
  • The soluble sperm factor that activates the egg: PLCzeta and beyond.
    Reproduction (IF 3.206) Pub Date : 2020-07-01
    Karl Swann

    PLCzeta(ζ) initiates Ca2+ oscillations and egg activation at fertilization in mammals, but studies in mouse eggs fertilized by PLCζ knockout (KO) sperm imply that there is another slow acting factor causing Ca2+ release. Here, I propose a hypothesis for how this second sperm factor might cause Ca2+ oscillations in mouse eggs.

    更新日期:2020-06-09
  • Evidence that activin A directly modulates early human male germline differentiation status.
    Reproduction (IF 3.206) Pub Date : 2020-07-01
    Sarah C Moody,Shoichi Wakitani,Julia C Young,Patrick S Western,Kate L Loveland

    Disrupted fetal germline development underpins testicular germ cell neoplasia, which is increasing worldwide. The complex signaling milieu during normal testis development includes TGFβ superfamily ligands; this study tests the hypothesis that, activin A, a TGFβ superfamily member, can influence gonocyte development. The human seminoma-derived cell line, TCam-2, a model of fetal gonocytes, was cultured

    更新日期:2020-06-09
  • The impact of embryo binucleation depends upon its origin.
    Reproduction (IF 3.206) Pub Date : 2020-07-01
    Lia Mara Gomes Paim,Greg FitzHarris

    Preimplantation embryos frequently contain binucleated cells, but reports differ as to whether binucleation affects development and whether such embryos should be used clinically. In this Point Of View article, we propose a possible explanation for this disparity: binucleation can arise by distinct routes, one that produces healthy blastomeres and one that directly threatens embryo viability.

    更新日期:2020-06-09
  • Culture conditions antagonize lineage-promoting signaling in the mouse blastocyst.
    Reproduction (IF 3.206) Pub Date : 2020-07-01
    Tristan Frum,Amy Ralston

    The mouse preimplantation embryo is a paradigm for discovery of the molecular principles governing formation of specific cell types during development. In this Point of View Article, we show that conditions commonly used for ex vivo culture of preimplantation development are themselves antagonistic to a pathway that is critical for blastocyst lineage commitment.

    更新日期:2020-06-09
  • Effect of nerve growth factor on the proliferation in newborn bovine testicular Sertoli cells.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Qiaoge Niu,Maosheng Cao,Chenfeng Yuan,Yuwen Huang,Zijiao Zhao,Boqi Zhang,Xin Wang,Yameng Wei,Wenjing Fan,Xu Zhou,Chunjin Li

    Nerve growth factor (NGF) has been proved to play important roles in male reproductive physiology, but the molecular mechanisms of NGF action remain unclear. In this study, the effects of NGF on the growth of newborn bovine testicular Sertoli (NBS) cells and the related signaling pathways were investigated. The NBS cells were treated in vitro with NGF (100 ng/mL) for 18 h. The expression levels of

    更新日期:2020-06-01
  • Patterns of MTT reduction in mammalian spermatozoa.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Robert John Aitken,Diatsendoula Gregoratos,Leslie Kutzera,Emma Towney,Minjie Lin,Alexandra Wilkins,Zamira Gibb

    MTT is widely used in biology as a probe for cell viability by virtue of its ability to generate deposits of insoluble formazan at sites of intense oxidoreductase activity. This response is generally held to reflect mitochondrial redox activity; however, extra-mitochondrial MTT reduction has also been recorded in certain cell types. Given this background, we set out to determine the major sites of

    更新日期:2020-06-01
  • CDC42 drives RHOA activity and actin polymerization during capacitation.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Tania Reyes-Miguel,Ana L Roa-Espitia,Rafael Baltiérrez-Hoyos,Enrique O Hernández-González

    Mammalian sperm cells acquire fertilizing capacity as a result of a process termed capacitation. Actin polymerization is important for capacitation; inhibiting actin polymerization prevents the adhesion and fusion of the sperm with the ovule. The main function of RHO proteins CDC42 and RHOA is to direct actin polymerization. Although these two RHO proteins are present in mammalian sperm, little is

    更新日期:2020-06-01
  • Adipokines as biomarkers of postpartum subclinical endometritis in dairy cows.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Gonçalo Pereira,Ricardo Bexiga,João Chagas E Silva,Elisabete Silva,Christelle Ramé,Joëlle Dupont,Yongzhi Guo,Patrice Humblot,Luís Lopes-da-Costa

    Adipokines emerged as regulators of metabolism and inflammation in several scenarios. This study evaluated the relationship between adipokines (adiponectin, chemerin and visfatin) and cytological (subclinical) endometritis, by comparing healthy (without), transient (recovered by 45 days postpartum (DPP)) and persistent (until 45 DPP) endometritis cows (n = 49). Cows with persistent endometritis had

    更新日期:2020-06-01
  • TET3 overexpression facilitates DNA reprogramming and early development of bovine SCNT embryos.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Jian Zhang,Linlin Hao,Qian Wei,Sheng Zhang,Hui Cheng,Yanhui Zhai,Yu Jiang,Xinglan An,Ziyi Li,Xueming Zhang,Bo Tang

    Somatic cell nuclear transfer (SCNT) has been successfully used for cloning in a variety of mammalian species. However, SCNT reprogramming efficiency is relatively low, in part, due to incomplete DNA methylation reprogramming of donor cell nuclei. We previously showed that ten-eleven translocation 3 (TET3) is responsible for active DNA demethylation during preimplantation embryonic development in bovines

    更新日期:2020-06-01
  • YAP1 inhibits ovarian endometriosis stromal cell invasion through ESR2.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Cheng Zeng,Pei-Li Wu,Zhao-Tong Dong,Xin Li,Ying-Fang Zhou,Qing Xue

    Endometriosis is an estrogen-dependent disease, and estrogen receptor 2 (ESR2) plays a critical role in the pathogenesis of ovarian endometriosis by promoting cell invasion. Yes-associated protein 1 (YAP1) plays suppressive roles in several types of tumors. However, the relationship between YAP1 and ESR2 is not fully understood. The aim of this study was to investigate the regulatory mechanism of YAP1

    更新日期:2020-06-01
  • Reduced retinoic acid synthesis accelerates prophase I and follicle activation.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Roseanne Rosario,Hazel L Stewart,Emily Walshe,Richard A Anderson

    In female mammals, reproductive potential is determined during fetal life by the formation of a non-renewable pool of primordial follicles. Initiation of meiosis is one of the defining features of germ cell differentiation and is well established to commence in response to retinoic acid. WIN 18,446 inhibits the conversion of retinol to retinoic acid, and therefore it was used to explore the impact

    更新日期:2020-06-01
  • Apomorphine induces mitochondrial-dysfunction-dependent apoptosis in choriocarcinoma.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Jin-Young Lee,Jiyeon Ham,Whasun Lim,Gwonhwa Song

    Apomorphine is a derivative of morphine that is used for the treatment of Parkinson's disease because of its effects on the hypothalamus. Therapeutic effects of apomorphine have also been reported for various neurological diseases and cancers. However, the molecular mechanisms of the antitumor effects of apomorphine are not clear, especially with respect to choriocarcinoma. This is the first study

    更新日期:2020-06-01
  • Rat postnatal prostate development is impaired by in vitro high-glucose environment.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Isabella Silva Cassimiro,Amanda Rodrigues Cruz,Beatriz Pelegrini Bosque,Laura Calazans de Melo Gomes,Renata Graciele Zanon,Jéssica Regina da Costa Silva,Patrícia Tieme Fujimura,Carlos Ueira-Vieira,Daniele Lisboa Ribeiro

    The prostate development has an important postnatal period where cell proliferation begins at the first days after birth and is related to gland growth and ramification. Any metabolic and/or hormonal changes occurring during the postnatal period can interfere with prostate branching. Hyperglycemia is a common condition in low-weight preterm babies at neonatal period and also a disorder found in the

    更新日期:2020-06-01
  • Mutation of rat Zp2 causes ROS-mediated oocyte apoptosis.
    Reproduction (IF 3.206) Pub Date : 2020-09-01
    Yan Wang,Hualin Huang,Minghua Zeng,Ru-Ping Quan,Jun-Ting Yang,Dan Guo,Ying Sun,Hongwen Deng,Hongmei Xiao

    In this study, we investigated a gene-edited (Zp2MT/MT) rat model of infertility caused by the failure to express the zona pellucida glycoprotein 2 (ZP2) due to the significant reduction of mRNA amount. We examined the defects in the zona pellucida (ZP) caused by ZP2 nullification and the influence of these defects on aspects of oocyte development, including apoptosis and fertilization ability. To

    更新日期:2020-06-01
  • The establishment of the fertile fish lineages derived from distant hybridization by overcoming the reproductive barriers.
    Reproduction (IF 3.206) Pub Date : 2020-06-01
    Qingfeng Liu,Junmei Liu,Liujiao Yuan,Lu Li,Min Tao,Chun Zhang,Qinbo Qin,Bo Chen,Ming Ma,Chenchen Tang,Shaojun Liu

    Distant hybridization refers to the cross between two different species or higher-ranking taxa. It is very significant if the new lineages with genetic variation, fertile ability, and improved characteristics can be established through distant hybridization. However, reproductive barriers are key limitations that must be overcome to establish fertile lineages derived from distant hybridization. In

    更新日期:2020-06-01
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