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The N-terminal modification of HORMAD2 causes its ectopic persistence on synapsed chromosomes without meiotic blockade Reproduction (IF 3.8) Pub Date : 2024-04-01 Isabella G Cossu, N Adrian Leu, Yongjuan Guan, P Jeremy Wang
In brief The dissociation of HORMA domain protein 2 (HORMAD2) from the synaptonemal complex is tightly regulated. This study reveals that the N-terminal region of HORMAD2 is critical for its dissociation from synapsed meiotic chromosomes. Abstract During meiosis, homologous chromosomes undergo synapsis and recombination. HORMA domain proteins regulate key processes in meiosis. Mammalian HORMAD1 and
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Paternal protein provisioning to embryos during male seahorse pregnancy Reproduction (IF 3.8) Pub Date : 2024-04-01 Zoe M G Skalkos, James U Van Dyke, Samson N Dowland, Camilla M Whittington
In brief Seahorses exhibit male pregnancy and are thus valuable comparative models for the study of the physiology and evolution of pregnancy. This study shows that protein is transported from fathers to developing embryos during gestation, and provides new knowledge about paternal contributions to embryonic development. Abstract Syngnathid embryos (seahorses, pipefishes and seadragons) develop on
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Naked mole-rat ovaries allow investigation of ovarian reserve in vitro germ cell expansion, and oocyte IVM within a single sample Reproduction (IF 3.8) Pub Date : 2024-03-01 Gretchen M Rosado, Ana Martinez-Marchal, Mariela Faykoo-Martinez, Melissa M. Holmes, Miguel Angel Angel Brieño-Enriquez
Recently, we described that in the naked mole-rat ovary it is possible to study the ovarian reserve and the mitotic expansion of the germ cell postnatally. Herein, we show oocyte in vitro maturation and in vitro germ cell expansion using the same ovary.
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The central role of pyruvate metabolism on the epigenetic maturation and transcriptional profile of bovine oocytes Reproduction (IF 3.8) Pub Date : 2024-04-01 João Vitor Alcantara da Silva, Jessica Ispada, Ricardo Perecin Nociti, Aldcejam Martins da Fonseca Junior, Camila Bruna de Lima, Erika Cristina dos Santos, Marcos Roberto Chiaratti, Marcella Pecora Milazzotto
In brief Pyruvate metabolism is one of the main metabolic pathways during oocyte maturation. This study demonstrates that pyruvate metabolism also regulates the epigenetic and molecular maturation in bovine oocytes. Abstract Pyruvate, the final product of glycolysis, undergoes conversion into acetyl-CoA within the mitochondria of oocytes, serving as a primary fuel source for the tricarboxylic acid
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Current Insights and a Potential Role of NAD in the Reproductive Health of Aging Fathers and Their Children Reproduction (IF 3.8) Pub Date : 2024-03-01 Morgan B Feuz, David Colton Nelson, Laura B Miller, Alexie E Zwerdling, R.g. Meyer, Mirella L Meyer-Ficca
In brief: In light of the increasing age of first-time fathers, this article summarizes the current scientific knowledge base on reproductive aging in the male, including sperm quality and health impacts for the offspring. The emerging role of NAD decline in reproductive aging is highlighted. Abstract: Over the past decades, the age of first-time fathers has been steadily increasing due to socio-economic
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Inhibition of HSP90AA1 induces abnormalities in bovine oocyte maturation and embryonic development Reproduction (IF 3.8) Pub Date : 2024-03-01 Baobao Zhao, Heqiang Li, Han Zhang, Xin Rui Lan, Xingchen Ren, Liangyi Zhang, Huiming Ma, Yong Zhang, Yongsheng Wang
In brief: HSP90AA1 is a ubiquitous molecular chaperone that can resist cellular stress, such as oxidative stress and apoptosis, and mediate the efficacy and protein folding of normal cells during heat stress, as well as many other functions. This study further reveals the role of HSP90AA1 in bovine oocytes maturation and early embryonic development. Abstract: HSP90AA1, a highly abundant and ubiquitous
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Congenital Zika Virus Infection Impacts on Male Mice Offspring's Reproductive Biology Reproduction (IF 3.8) Pub Date : 2024-03-01 Natália Teixeira Wnuk, André Felipe Almeida Figueiredo, Talita De Oliveira Farias, Marcos Rocha Gouvêa Brener, Samyra Maria dos Santos Nassif Lacerda, Vidyleison Neves Camargos, Paulo Henrique Amaral, Lidia Maria Andrade, Maria Ivonete Nogueira Silva, Roberta Araujo Lopes, Raphael Escorsim Szawka, Juan Carlos González, Mauro Martins Teixeira, Danielle da Glória Souza, Vivian Vasconcelos Costa, Guilherme
Infection with ZIKV during pregnancy is associated with fetal developmental problems. Although neurological issues are being explored more in experimental studies, limited research has focused on the reproductive health consequences for offspring born to infected mothers. In this context, this study aimed to assess the impact of ZIKV infection during pregnancy on the testes and sperm of adult male
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Impaired Fertility in Adenomyosis: a Murine Model Reveals Endometrial Receptivity and Progesterone Resistance Imbalances Reproduction (IF 3.8) Pub Date : 2024-03-01 Marlyne Squatrito, Julie Vervier, Jules Bindels, Laëtitia Bernet, Silvia Blacher, Michelle Nisolle, Carine Munaut
This study investigates the intricate relationship between adenomyosis and reproductive health using a murine model, offering novel insights into this prevalent gynecological disorder. Adenomyosis, characterized by the invasive growth of endometrial tissue into the myometrium, is believed to negatively impact fertility. However, the challenge lies in disentangling this influence, as adenomyosis often
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Oviduct and endometrial epithelium improve in vitro produced bovine embryo developmental kinetics. Reproduction (IF 3.8) Pub Date : 2024-03-01 L Kirsten Senn, Katheryn D Peterson, J Lannett Edwards, Rebecca R Payton, Daniel J Mathew
In vitro embryo production in cattle greatly impacts blastomere biochemistry, embryo rate of development and pre- and post-transfer survival. In vivo, the bovine embryo migrates through the oviduct isthmus before entering the uterus on approximately Day 4 of development where it remains unattached within the uterine lumen until Day 20 of gestation. During this time, the embryo is sequentially exposed
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Peroxisome proliferator-activated receptors and placental function in women with polycystic ovary syndrome Reproduction (IF 3.8) Pub Date : 2024-03-01 Aimé Silva, Alicia Motta
Polycystic ovary syndrome (PCOS) is one of the major endocrine disorders affecting women during their reproductive ages. Given its association with other pathologies, such as insulin resistance, metabolic syndrome, type 2 diabetes and obesity, women with PCOS could present high-risk pregnancies, including a high abortion rate, implantation failure, an increased risk of gestational diabetes, preeclampsia
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Does TFAP2C govern conflicting cell fates in mouse preimplantation embryos? Reproduction (IF 3.8) Pub Date : 2024-04-01 Chad S Driscoll, Jaehwan Kim, Mohamed Ashry, Jason G Knott
Transcription factor AP2 gamma (TFAP2C) is a well-established regulator of the trophoblast lineage in mice and humans, but a handful of studies indicate that TFAP2C may play an important role in pluripotency. Here, we hypothesize and provide new evidence that TFAP2C functions as an activator of trophoblast and pluripotency genes during preimplantation embryo development.
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Development of polarity-reversed endometrial epithelial organoids Reproduction (IF 3.8) Pub Date : 2024-03-01 Vakil Ahmad, Sai Goutham Reddy Yeddula, Bhanu P Telugu, Thomas E Spencer, Andrew M Kelleher
In brief Polarity-reversed endometrial epithelial organoids exhibit histological and physiological characteristics resembling uterine epithelium in vivo, respond to hormones, and undergo secretory cell transformation. The ability to modify the polarity without impairing functionality, coupled with successful coculture with microbial and embryonic entities, paves the way for investigating complex interactions
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DOHaD: A Menagerie of Adaptations and Perspectives: Long-lasting effects of the presence of male siblings in utero on subsequent reproductive performance Reproduction (IF 3.8) Pub Date : 2024-03-01 Mathieu Douhard, Victor Ronget, Frédéric Douhard
In brief In litter-bearing species, developing offspring can be exposed to different concentrations of androgens and oestrogens according to the sex of neighbouring fetuses. However, the relationships between litter sex composition and subsequent reproductive performance are discordant and complex. Abstract Laboratory studies with rodents indicate that in utero proximity of a female to male fetus can
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The press-assisted fusion scheme significantly mitigates the quantity of HVJ-E required in mitochondrial replacement techniques Reproduction (IF 3.8) Pub Date : 2024-03-01 Meng Ma, Shutian Jiang, Wei Jin, Wenzhi Li, Chen Chen, Kaibo Lin, Xiaoyu Liao, Danjun Li, Yanping Kuang, Qifeng Lyu
In brief The impact of HVJ-E employed in mitochondrial replacement techniques (MRTs) on embryonic development remains uncertain. This study has exhibited the influence of HVJ-E utilized in MRTs on embryonic development and has devised a novel HVJ-E-induced fusion approach to curtail the amount of HVJ-E employed in MRTs. Abstract Mitochondrial replacement techniques (MRTs) provide a viable option for
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Decreased NSD2 impairs stromal cell proliferation in human endometrium via reprogramming H3K36me2 Reproduction (IF 3.8) Pub Date : 2024-03-01 Chuan-Mei Qin, Xiao-Wei Wei, Jia-Yi Wu, Xue-Qing Liu, Yi Lin
In brief The proliferation of the endometrium is regulated by histone methylation. This study shows that decreased NSD2 impairs proliferative-phase endometrial stromal cell proliferation in patients with recurrent implantation failure via epigenetic reprogramming of H3K36me2 methylation on the promoter region of MCM7. Abstract Recurrent implantation failure (RIF) is a formidable challenge in assisted
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Oocyte activation is a cytoplasm-confined event so far: what about the nucleus? Reproduction (IF 3.8) Pub Date : 2024-03-01 Luisa Gioia, Luca Palazzese, Marta Czernik, Domenico Iuso, Helena Fulka, Josef Fulka Jr, Pasqualino Loi
The fertilizing spermatozoa induce a Ca2+ oscillatory pattern, the universal hallmark of oocyte activation, in all sexually reproducing animals. Assisted reproductive technologies (ARTs) like intracytoplasmic sperm injection (ICSI) bypass the physiological pathway; however, while a normal Ca2+ release pattern occurs in some species, particularly humans, artificial activation is compulsory for ICSI-fertilized
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Investigating ticagrelor in a preclinical pipeline as a novel therapeutic to prevent preterm birth Reproduction (IF 3.8) Pub Date : 2024-03-01 Bridget M Arman, Natalie K Binder, Natasha de Alwis, Sally Beard, Anjali Garg, Tu’uhevaha J Kaitu’u-Lino, Natalie J Hannan
In brief Preterm birth is the leading cause of perinatal morbidity and mortality, and new therapies that delay preterm birth and improve neonatal outcomes are urgently needed. This study investigates whether ticagrelor inhibits uterine contractility and inflammation in preclinical in vitro, ex vivo (human) and in vivo (mouse) studies, to explore the potential of repurposing ticagrelor for the prevention
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TEAD4 regulates KRT8 and YAP in preimplantation embryos in mice but not in cattle Reproduction (IF 3.8) Pub Date : 2024-03-01 Xiaotong Wu, Yan Shi, Bingjie Hu, Panpan Zhao, Shuang Li, Lieying Xiao, Shaohua Wang, Kun Zhang
In brief Lineage specification plays a vital role in preimplantation development. TEAD4 is an essential transcription factor for trophectoderm lineage specification in mice but not in cattle. Abstract Tead4, a critical transcription factor expressed during preimplantation development, is essential for the expression of trophectoderm-specific genes in mice. However, the functional mechanism of TEAD4
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Isolation of serum-derived placental/amniochorionic extracellular vesicles across pregnancy by immunoaffinity using PLAP and HLA-G Reproduction (IF 3.8) Pub Date : 2024-02-01 Uma Shinde, Aishwarya Rao, Vandana Bansal, Dhanjit Kumar Das, Nafisa Huseni Balasinor, Taruna Madan
Extracellular vesicles (EVs) are membrane-bound nanovesicles secreted from the cells into extracellular space and body fluids. They are considered “fingerprints of parent cells” which can reflect their physiological and functional states. During pregnancy, extracellular vesicles (EVs) are produced by the syncytiotrophoblasts and extravillous trophoblasts and are released into the maternal bloodstream
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Spindle shape and volume differ in high- and low-quality metaphase II oocytes Reproduction (IF 3.8) Pub Date : 2024-02-01 Monika Fluks, Robert Milewski, Szymon Tamborski, Maciej Szkulmowski, Anna Ajduk
The proper development of embryos strongly depends on the quality of oocytes, so the evaluation of oocytes may be a useful initial step in in vitro fertilization procedures. Additionally, it enables embryologists to make more informed decisions regarding the treatments chosen for the patients and better manage patients’ expectations. Optical coherence microscopy (OCM) allows for non-invasive 3D visualization
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PPAR-gamma influences developmental competence and trophectoderm lineage specification in bovine embryos Reproduction (IF 3.8) Pub Date : 2024-02-01 Maura S McGraw, Sandeep K Rajput, Bradford W Daigneault
In brief Peroxisome proliferator-activated receptor gamma (PPARG) is a critical regulator of placental function, but earlier roles in preimplantation embryo development and embryonic origins of placental formation have not been established. Results herein demonstrate that PPARG responds to pharmacologic stimulation in the bovine preimplantation embryo and influences blastocyst development, cell lineage
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Spermatogonial depletion and a spermatogenesis defect in the Dp(16)1Yey mouse model of Down syndrome Reproduction (IF 3.8) Pub Date : 2024-02-01 Rodolphe Dard, Anastasia Tutunaru, Malek Bouassida, Aissatu Balde Camara, Estelle Parizot, Nadim Kassis, Joanne Fortemps, Céline Cierniewski, Chrystele Racine, Nathalie Di-Clemente, François Vialard, Nathalie Janel
In brief Little is known about the mechanism responsible for male infertility in Down syndrome. Duplication of Mmu16 chromosome (an orthologue of Hsa21, bearing Dyrk1A) in Dp(16)1Yey mice is responsible for impaired spermatogenesis through spermatogonial depletion and hypogonadotropic hypogonadism. Abstract Down syndrome (DS), or trisomy 21, is the most common chromosomal disorder in humans. Men with
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Ca2 + concentrations in mouse sperm mitochondria fluctuate according to the cytosol Reproduction (IF 3.8) Pub Date : 2024-02-01 Yoloxochitl Sánchez-Guevara, Enrique I Oliver, Takuya Nishigaki
In brief Free Ca2+ concentrations within mitochondria ([Ca2+]mt) in mouse spermatozoa were reported in a micromolar range based on the result obtained with Calcium Green 5N. The results obtained with Rhod-2, Fluo-3, and Fluo-5N suggest that the [Ca2+]mt are comparable to the cytosolic levels at the resting condition and under the condition stimulated by ATP. Abstract Mitochondria are important organelles
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Immunohistochemical examination of the uteroplacental interface of cows on days 21, 31, 40, and 67 of gestation Reproduction (IF 3.8) Pub Date : 2024-02-01 Heewon Seo, Gabriela D Melo, Ramiro V Oliveira, Gessica A Franco-Johannsen, Fuller W Bazer, Ky G Pohler, Gregory A Johnson
In brief Cattle are classified as having synepitheliochorial placentation in which the majority of the uterine luminal epithelial cells remain intact with some luminal epithelial cells fusing with binucleate trophoblast cells to form syncytial trinucleate cells. This study suggests the possibility that, for a limited and as yet undefined period of gestation, the majority of luminal epithelial cells
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Morphological analysis of the human sperm tail during the early post-fertilization stage Reproduction (IF 3.8) Pub Date : 2024-02-01 Yoshiteru Kai, Hachiro Iseki, Takeshi Matsui, Naoki Yamashita
After fertilization, the human sperm tail lost its fibrous sheath at the pronuclear stage to become coiled and then attached to one of the first mitotic spindle poles. The tip of the sperm tail was branched and its degree did not change from the pronuclear stage to the second mitosis.
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MicroRNA-146b negatively affects bovine embryo development and quality Reproduction (IF 3.8) Pub Date : 2024-02-01 Krishna Chaitanya Pavani, Guan XueFeng, Jayendra Chunduru, Tim Meese, Luc J Peelman, Filip Van Nieuwerburgh, Dieter Deforce, An Hendrix, Kelly Tilleman, Ann Van Soom, Katrien Smits
In brief MicroRNAs (miRNAs) carried in extracellular vesicles are one of the essential factors for embryo development. This study demonstrates that miRNA-146 b has negative impacts on the quality and development of bovine embryos and also shows its higher expression in non-blastocyst embryos and related EVs. Abstract MicroRNAs (miRNAs), which can be carried inside extracellular vesicles (EVs), play
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BMAL1 positively correlates with genes regulating steroidogenesis in human luteinized granulosa cells Reproduction (IF 3.8) Pub Date : 2024-02-01 Tomomi Kawamura, Yidan Dai, Masanori Ono, Takayuki Kikuchi, Akina Yamanaka, Keiko Ueno, Junya Kojima, Tomoko Fujiwara, Takiko Daikoku, Yoshiko Maida, Hitoshi Ando, Hiroshi Fujiwara, Naoaki Kuji, Hirotaka Nishi
In brief In this study, we examined the relationship between BMAL1 expression and the genes regulating steroid biosynthesis in human luteinized granulosa cells. BMAL1 function is crucial for steroid production and proper ovarian function, highlighting the importance of circadian clock regulation in female reproductive health. Abstract Human luteinized granulosa cells were collected to analyze circadian
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Mitochondrial uncoupling proteins regulate the metabolic function of human Sertoli cells Reproduction (IF 3.8) Pub Date : 2024-02-01 David F Carrageta, Laís Freire-Brito, Bárbara Guerra-Carvalho, Raquel L Bernardino, Bruno S Monteiro, Alberto Barros, Pedro F Oliveira, Mariana P Monteiro, Marco G Alves
In brief Mitochondrial uncoupling proteins (UCPs) regulate mitochondrial activity and reactive oxygen species production through the transport of protons and metabolites. This study identified the expression of UCPs in human Sertoli cells, which proved to be modulators of their mitochondrial activity. Abstract Mitochondrial uncoupling proteins (UCPs) are mitochondrial channels responsible for the transport
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In vitro reduction of bovine oocyte ATP production with oligomycin affects embryo epigenome Reproduction (IF 3.8) Pub Date : 2024-02-01 Ben Meulders, Jo L M R Leroy, Inne Xhonneux, Peter E J Bols, Waleed F A Marei
In brief Epigenetic programming is a crucial process during early embryo development that can have a significant impact on the results of assisted reproductive technology (ART) and offspring health. Here we show evidence using a bovine in vitro experiment that embryo epigenetic programing is dependent on oocyte mitochondrial bioenergetic activity during maturation. Abstract This study investigated
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Generation of porcine endometrial organoids and their use as a model for enhancing embryonic attachment and elongation Reproduction (IF 3.8) Pub Date : 2024-02-01 Islam M Saadeldin, Ayeong Han, Seonggyu Bang, Heejae Kang, Heyyoung Kim, Mariam M Abady, Ji-Seon Jeong, Ha-Jeong Kwon, Sanghoon Lee, Jongki Cho
In brief Porcine endometrial organoids (EOs) were isolated and characterized, revealing distinctive features such as unique extracellular matrix formation, fusion into uterine bud-like structures, and facilitation of embryo elongation. The yield of EOs was significantly enhanced by cryopreservation medium supplemented with the rock inhibitor (Y-27632), resulting in reduced expression of apoptotic mRNAs
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Ultrastructural cilia defects in multi-ciliated uterine glandular epithelial cells from women with reproductive failure Reproduction (IF 3.8) Pub Date : 2024-01-01 Jennifer E Pearson-Farr, Regan Doherty, David S Chatelet, Patricia Goggin, Ka Ying Bonnie Ng, Jane S Lucas, Jane K Cleal, Ying C Cheong, Rohan M Lewis
In brief The causes of subfertility and recurrent pregnancy loss are often unclear. This study shows that endometrial gland cilia from women with subfertility have ultrastructural defects. Abstract Endometrial glands secrete products into the endometrium and are necessary for embryo implantation and successful pregnancy. However, structural and functional abnormalities in endometrial gland cilia from
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The concentration of glucose in the media influences the susceptibility of stallion spermatozoa to ferroptosis Reproduction (IF 3.8) Pub Date : 2024-01-01 Francisco E Martín-Cano, Gemma Gaitskell-Phillips, Eva da Silva-Álvarez, Antonio Silva-Rodríguez, Alba Castillejo-Rufo, José Antonio Tapia, María Cruz Gil, Cristina Ortega-Ferrusola, Fernando J Peña
In brief Although common in many commercial extenders, supraphysiological concentrations of glucose in the media may be detrimental to stallion spermatozoa. In this study, we present evidence that these elevated glucose levels may predispose spermatozoa to ferroptosis. Abstract Stallion spermatozoa depend on oxidative phosphorylation as their major source of ATP; however, the metabolism of these cells
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Endocannabinoid regulation in the cervix during pregnancy: insights into molecular mechanisms of premature labor Reproduction (IF 3.8) Pub Date : 2024-01-01 Carolina Marvaldi, Felisa María Herrero Lo Giudice, Clare Johnson, Julieta Schander, Fernando Correa, Maximiliano Cella, Julieta Aisemberg, Ana María Franchi, Heather Bradshaw, Manuel Luis Wolfson
During pregnancy, the main role of the cervix is to isolate the fetus from outside pathogens and maintain the relatively closed system of uterine gestation. Conversely, towards the end of pregnancy, the cervix must be remodeled to increase flexibility and allow the delivery. This process is called cervical remodeling and dysregulation of the process plays a role in premature delivery. The endocannabinoidome
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Effect of vitrification on protein O-GlcNAcylation in mouse metaphase II oocytes Reproduction (IF 3.8) Pub Date : 2024-01-01 Kang Zhang, Tianyang Lan, Feifei Lin, Ruichuan Liu, Qifu He, Feng Gao, Shenghui Wu, Jian Kang, Hongmin Li, Fusheng Quan
In brief Oocyte vitrification leads to DNA hypomethylation, which results in defect in early embryo development. This study reveals that oocyte vitrification impairs the DNA methylation pattern by influencing protein O-GlcNAcylation. Abstract Oocyte vitrification leads to decreased DNA methylation levels, which impairs the quality and the developmental potential of oocytes. However, the underlying
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Transcriptional profiling of Kiss1 neurons from arcuate and rostral periventricular hypothalamic regions in female mice Reproduction (IF 3.8) Pub Date : 2024-01-01 Stephen Manchishi, Malwina Prater, William Henry Colledge
In brief The transcriptional profiles of Kiss1 neurons from the arcuate and the rostral periventricular region of the third ventricle of the hypothalamus have been directly compared in diestrous female mice. Differentially expressed genes provide molecular signatures for these two populations of Kiss1 neurons and insights into their physiology. Abstract The neuropeptide kisspeptin is produced by Kiss1
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Downregulation of CRHBP is associated with trophoblast invasion inhibition in recurrent pregnancy loss Reproduction (IF 3.8) Pub Date : 2024-01-01 Fengrun Sun, Liyuan Cui, Jinfeng Qian, Meirong Du, Songcun Wang
In brief Corticotropin-releasing hormone binding protein (CRHBP) is fundamental to the stress response and plays an important role in parturition during pregnancy. This study shows that abnormal CRHBP expression could be an early warning sign of recurrent pregnancy loss and that CRHBP knockdown could suppress HTR8/SVneo cell invasion by the PKC signaling pathway via interacting with CRH receptor 2
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Adipolin (C1QTNF12) is a new adipokine in female reproduction: expression and function in porcine granulosa cells Reproduction (IF 3.8) Pub Date : 2024-01-01 Alix Barbe, Patrycja Kurowska, Christelle Rame, Pascal Froment, Agnieszka Rak, Joëlle Dupont
In brief Adipolin (C1QTNF12) has been described as a regulator of metabolism and is linked with the pathophysiology of PCOS. In this study, for the first time, we show the expression of C1QTNF12 in granulosa cells and its positive effect on porcine granulosa cell proliferation and steroid synthesis. Abstract Adipolin (C1QTNF12) is a recently discovered adipokine that plays an important role in glucose
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The dogma of the sterile uterus revisited: does microbial seeding occur during fetal life in humans and animals? Reproduction (IF 3.8) Pub Date : 2024-01-01 Penelope Banchi, Barbara Colitti, Geert Opsomer, Ada Rota, Ann Van Soom
In brief Opposing conclusions have been drawn regarding the presence of viable bacteria in the healthy pregnant uterus. Current evidence in humans and animals suggests that fetomaternal tissues present only traces of bacteria whose viability is still to be proven. Abstract The debate about the pioneer colonization of the fetus is still open, being the ‘in utero colonization’ hypothesis versus the ‘sterile
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Regional abundances of binder of sperm (BSP) proteins are negatively associated with the quality of frozen-thawed bovine spermatozoa Reproduction (IF 3.8) Pub Date : 2024-01-01 Edgar A Diaz-Miranda, Lauren E Hamilton, Michal Zigo, Lindsey Fallon, M Sofia Ortega, Mayra E Ortiz D’Avila Assumpção, José D Guimarães, Peter Sutovsky
In brief The localization and abundance of the sperm BSP proteins correlate with in vitro fertility in domestic bulls used in artificial insemination service. Abstract Binder of sperm (BSP) proteins, secreted mainly by the accessory sex glands, are the major protein family present in bovine seminal plasma and on the sperm surface after ejaculation. In vivo, BSP proteins facilitate sperm capacitation
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The other side of capacitation: role of mouse male molecules in the regulation of time and place of capacitation Reproduction (IF 3.8) Pub Date : 2023-12-01 Anabella R Nicolli, Andreina Cesari
In brief Capacitation is regulated by decapacitation factors secreted by male ducts and accessory sex glands. This revision is focused on targets and events regulated by decapacitation factors in Mus musculus and their potential use for fertility control. Abstract Sperm capacitation is a necessary process for mammalian spermatozoa to acquire fertilization capability. This process occurs when the sperm
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Post-ovulatory aging affects mitochondria, spindle and protein metabolism in mouse oocytes Reproduction (IF 3.8) Pub Date : 2023-12-01 Chuanxin Zhang, Xueqi Dong, Xinyi Yuan, Jinzhu Song, Jiawei Wang, Xiaoyu Yin, Zhenzhen Hou, Cheng Li, Shuiying Ma, Zi-Jiang Chen, Keliang Wu
In brief Post-ovulatory aging (POA) results in a decline in oocyte quality and embryonic developmental capacity although the underlying mechanisms remain elusive. This study provides comprehensive mRNA expression profiles of fresh and aging oocytes in mice for the first time. Abstract POA impairs the quality of mammalian oocytes with harmful effects on the developmental potential of the embryo. This
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Genistein ameliorates starvation-induced porcine follicular granulosa cell apoptosis Reproduction (IF 3.8) Pub Date : 2023-12-01 Guoyun Wu, Dan Song, Huadong Wu, Fang Zhao, Wei Ding, Zhe Wang, Fangxiong Shi, Quanwei Wei
In brief Genistein contributes to granulosa cell (GC) survival by two routes: one is that genistein induced p-AMPK and inhibited p-mTOR, which induces LC3 activation and autophagy; the other is that genistein inhibited caspase-3 and its cleavage, which induces PARP1 activation and PARylation. Abstract Genistein is an isoflavone which is beneficial for health, but little is known regarding its function
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Unilateral lesion of the suprachiasmatic nucleus impairs estradiol feedback, follicular development, estrous cycle and ovulation Reproduction (IF 3.8) Pub Date : 2023-12-01 Carlos-Camilo Silva, Diana Pamela Benítez, Angélica Flores, Georgina Daniela Cortés, Elizabeth Vieyra, Cinthia Rocío Juárez-Tapia, Jessica Romero-Reyes, Roberto Domínguez
In brief The SCN regulates ovulation by stimulating the preovulatory surge of gonadotropins. This study revealed an additional role in the sensitization of the hypothalamus to estradiol that changes along the estrous cycle and the side of the nucleus. Abstract Ovulation is timed by neural signals originating at the suprachiasmatic nucleus (SCN) that trigger ovulation when converge with high estradiol
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IFNT stimulates lysosomal function via type I IFN signaling pathway in pregnant bovine leukocytes Reproduction (IF 3.8) Pub Date : 2023-12-01 Md Abdus Shabur Talukder, Ahmed Zaky Balboula, Hanako Bai, Manabu Kawahara, Koji Kimura, Masashi Takahashi
In brief Interferon tau (IFNT) stimulates lysosomal activation via the Janus-activated kinase in peripheral blood leukocytes during pregnancy recognition. IFNT-mediated lysosomal activation could serve as a novel marker for early pregnancy in cattle. Abstract IFNT is important in establishing pregnancy in ruminants. Secreted IFNT in the uterus induces the expression of an interferon-stimulated gene
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CD74 deficiency reduces trophoblast invasion and proliferation mediated by SIRT1 in preeclampsia Reproduction (IF 3.8) Pub Date : 2023-12-01 Zhenzhen Liu, Jiangnan Pei, Xiaoyue Zhang, Chengjie Wang, Yao Tang, Haiyan Liu, Yi Yu, Shouling Luo, Weirong Gu
In brief Preeclampsia (PE) is a severe complication that leads to major maternal and fetal mortality and morbidity, and one of its causes is extravillous trophoblast (EVT) dysfunction. This study revealed the role of CD74 in the invasion and proliferation of EVTs. Abstract PE is a severe hypertensive disorder during pregnancy, and one of its causes is the dysfunction of EVTs. In this study, we analyzed
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New allele of mouse DNA/RNA helicase senataxin causes meiotic arrest and infertility Reproduction (IF 3.8) Pub Date : 2023-12-01 Yasuhiro Fujiwara, Kouhei Saito, Fengyun Sun, Sabrina Petri, Erina Inoue, John Schimenti, Yuki Okada, Mary Ann Handel
In brief A new allele of the senataxin gene Setxspcar3 causes meiotic arrest of spermatocytes with aberrant DNA damage and accumulation of R-loops. Abstract An unbiased screen for discovering novel mouse genes for fertility identified the spcar3, spermatocyte arrest 3, mutant phenotype. The spcar3 mutation identified a new allele of the Setx gene, encoding senataxin, a DNA/RNA helicase that regulates
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DCAF13 and RNF114 participate in the regulation of early porcine embryo development Reproduction (IF 3.8) Pub Date : 2023-12-01 Zigomar da Silva, Werner Giehl Glanzner, Karina Gutierrez, Mariana Priotto de Macedo, Vanessa Guay, Luke Currin, Maria Elena Herrera Carrillo, Paulo Bayard Dias Gonçalves, Vilceu Bordignon
In brief Ubiquitination plays a pivotal role in a multitude of cellular functions; however, the precise contributions of various ubiquitin ligases in governing early developmental processes remain largely unexplored. This study revealed that the E3 ubiquitin ligases DCAF13 and RNF114 are both necessary for the normal regulation of early porcine embryo development. Abstract Ubiquitylation is required
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In vitro fertilization program in white rhinoceros Reproduction (IF 3.8) Pub Date : 2023-12-01 Thomas Bernd Hildebrandt, Susanne Holtze, Silvia Colleoni, Robert Hermes, Jan Stejskal, Isaac Lekolool, David Ndeereh, Patrick Omondi, Linus Kariuki, Domnic Mijele, Samuel Mutisya, Stephen Ngulu, Sebastian Diecke, Katsuhiko Hayashi, Giovanna Lazzari, Barbara de Mori, Pierfrancesco Biasetti, Alessandra Quaggio, Cesare Galli, Frank Goeritz
In brief To save endangered rhinoceros species, assisted reproductive technologies are warranted. We here report in vitro blastocyst generation of the Near-Threatened Southern white rhinoceros and, for the first time, also of the technically Extinct Northern white rhinoceros. Abstract The Anthropocene is marked by a dramatic biodiversity decline, particularly affecting the family Rhinocerotidae. Three
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Mouse oocytes restore antral stage partial mechanical denudation in vitro Reproduction (IF 3.8) Pub Date : 2023-12-01 Anamaria-Cristina Herta, Nazli Akin, Ingrid Segers, Katy Billooye, Johan Smitz, Ellen Anckaert
In brief Partially denuded mouse cumulus–oocyte complexes restore likely functional transzonal projections in culture, under meiotic inhibition, with no detectable impact on oocyte competence. This proof-of-concept study constitutes positive premises for improving the developmental competence of human capacitation (CAPA)–in vitro maturation (IVM) oocytes with inadequate somatic cell connections. Abstract
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Sire modulates developmental kinetics and transcriptome of the bovine embryo Reproduction (IF 3.8) Pub Date : 2023-11-01 Kelsey Nicole Lockhart, Jessica N Drum, Ahmed Z Balboula, Christine M Spinka, Thomas E Spencer, M Sofia Ortega
In brief The first week of gestation is a period of major pregnancy loss in cattle, this study reveals that the male plays a key role in regulating embryonic development during this time. Abstract The impact of sire on preimplantation embryonic development in cattle remains poorly understood. This study evaluated differences in embryos produced in vitro from sires with varying capacities to produce
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Specc1l deficiency leads to abnormal oocyte meiosis and reduced blastocyst development in mouse Reproduction (IF 3.8) Pub Date : 2023-11-01 Seulah Lee, Inchul Choi
In brief Proper oocyte maturation is important in early embryo development. This study provides evidence that abnormal meiotic maturation can impact the developmental competency of preimplantation embryos. Abstract This study aimed to investigate the potential role of the mouse SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1 like), a microtubule and actin cytoskeleton-associated
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An abnormal LPA/LPAR1–NHE1 axis leads to the autophagy deficiency of trophoblast cells in recurrent spontaneous abortion Reproduction (IF 3.8) Pub Date : 2023-11-01 Min Chen, Jia-Lu Shi, Zi-Meng Zheng, Zhi Lin, Ming-Qing Li, Jun Shao
In brief Autophagy is important for trophoblast cells at the maternal–fetal interface during early pregnancy. This study suggests that trophoblast cells can promote the autophagy under a regulation of the LPA/LPAR 1–NHE1 axis. Abstract The autophagy of trophoblasts is necessary for developing and maintaining a healthy pregnancy. Autophagy dysfunction in trophoblast cells is linked to recurrent spontaneous
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Culture of vitrified bovine ovarian tissue on agarose gel inserts maintains follicle integrity Reproduction (IF 3.8) Pub Date : 2023-11-01 Isa Mohammed Alkali, Martina Colombo, Alberto Maria Luciano, Wojciech Nizanski, Hiba Ali Hassan, Piotr Dziegiel, Gaia Cecilia Luvoni
In brief Ovarian tissue cryopreservation and culture provide an option for fertility preservation without tissue grafting, but need optimization. This study reveals that vitrified bovine ovarian tissue can be cultured on agarose gel and maintain follicle morphology, low activation, and low apoptosis. Abstract Ovarian tissue preservation is hitherto a promising fertility insurance option for precious
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Maternal restricted- and over- feeding during gestation perturb offspring sperm epigenome in sheep Reproduction (IF 3.8) Pub Date : 2023-11-01 Linkai Zhu, Nicole Tillquist, Giovanna Scatolin, Rachael Gately, Mia Kawaida, Amanda Reiter, Sarah Reed, Steven Zinn, Kristen Govoni, Zongliang Jiang
In brief Inadequate maternal nutrition during gestation can have immediate and lifelong effects on offspring. This study shows that maternal restricted - and over- nutrition during gestation do not affect semen characteristics in F1 male offspring but alters offspring sperm sncRNA profiles and DNA methylome in sheep. Abstract There is a growing body of evidence that inadequate maternal nutrition during
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Emodin improves glucose metabolism and ovarian function in PCOS mice via the HMGB1/TLR4/NF-κB molecular pathway Reproduction (IF 3.8) Pub Date : 2023-11-01 Antonia Otoo, Armin Czika, Jones Lamptey, Jun-Pu Yang, Qian Feng, Mei-Jiao Wang, Ying-Xiong Wang, Yu-Bin Ding
In brief Obese PCOS mice display metabolic and endocrine disorders that manifest as abnormal metabolism of glucose and dysfunctions in the reproductive system. This study demonstrates that emodin alleviates most of these conditions possibly via the HMGB1/TLR4/NF-kB pathway. Abstract PCOS is a reproductive disorder with an unclear etiology. It affects 5–10% of women worldwide and is largely associated
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A long non-coding RNA essential for early embryonic development improves somatic cell nuclear transfer somatic cell nuclear transfer efficiency in goats Reproduction (IF 3.8) Pub Date : 2023-10-01 Miaomiao Jin, Lu Zhao, Hanwen Yang, Jianglin Zhao, Hongwei Ma, Yanzhi Chen, Jingcheng Zhang, Yan Luo, Yong Zhang, Jun Liu
In brief Early embryonic development in goats is a complex and an important process. This study identified a novel long non-coding RNA (lncRNA), lncRNA3720, that appears to affect early embryonic development in goats through histone variants. Abstract Although abundant lncRNAs have been found to be highly expressed in early embryos, the functions and mechanisms of most lncRNAs in regulating embryonic
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Murine models of advanced maternal age: a systematic review and meta-analysis Reproduction (IF 3.8) Pub Date : 2023-10-01 Jessica Dalton-O’Reilly, Alexander E P Heazell, Michelle Desforges, Susan Greenwood, Mark Dilworth
In brief Animal models have been developed to aid understanding of the increased incidence of adverse pregnancy complications observed in women of advanced maternal age (AMA). This systematic review of murine models of AMA demonstrates consistent effects of decreased litter size and fetal weight; this supports the future use of these models to determine pathophysiological mechanisms and test therapeutic
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Male minipuberty in human and non-human primates: planting the seeds of future fertility Reproduction (IF 3.8) Pub Date : 2023-10-01 Alexander S Busch, Juan Manuel Paturlanne, Nina Neuhaus, Joachim Wistuba, Stefan Schlatt, Anders Juul, Heymut Omran
In brief Minipuberty is a transient activity period of the hypothalamic–pituitary–gonadal axis in the postnatal and infant period including surging serum concentrations of reproductive hormones. Increasing evidence points to an important role of this period for maturation of the testes and thereby for male reproductive function. Abstract Minipuberty is a transient activity period of the hypothalam
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Molecular characterization of the follicular development of BMP15-edited pigs Reproduction (IF 3.8) Pub Date : 2023-10-01 Yafei Jiao, Tiantuan Jiang, Qiyuan Lin, Jinming Guo, Chang Bei, Peiqing Cong, Delin Mo, Xiaohong Liu, Yaosheng Chen, Zuyong He
In brief The regulatory role of BMP15 on porcine ovarian follicular development still remains unclear. This study reveals that biallelic editing of BMP15 impairs SMAD signaling and inhibits granulosa cell proliferation, resulting in porcine follicular development arrest and ovarian hypoplasia. Abstract Bone morphogenetic protein 15 (BMP15) is a member of the transforming growth factor beta (TGF-β)
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Porcine-specific expression of the three functional CYP19 paralogs in early conceptus, placenta, and gonads Reproduction (IF 3.8) Pub Date : 2023-10-01 Caroline Gomes Lucas, Riley M Sullivan, Caroline A Pfeiffer, Paula R Chen, JaeWoo Kim, Mariana Sponchiado, Alan J Conley, Randall S Prather, Kevin D Wells, Rodney D Geisert
In brief Aromatase catalyzes the synthesis of estrogens and has been shown to have an important role during the establishment of pregnancy in the pig. This study confirmed the differential expression of the three aromatase isoforms. Abstract Although three porcine aromatase isoforms have been identified, their gene expression profiles in reproduction are still poorly understood. Here, we identified