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Biochemical characterization and stability of Bacillus subtilis polygalacturonase produced using hazelnut shells by submerged fermentation Biocatal. Biotransform. (IF 1.863) Pub Date : 2021-01-13 Sibel Uzuner; Deniz Cekmecelioglu
Abstract Biochemical and bio-catalytic properties of an enzyme are essential to evaluate its potential industrial applications in food, pharmaceutical, textile, paper and pulp. More specifically, identifying optimal pH and temperature, and their effects on the residual enzyme activity are of high priority in biochemical characterisation. Therefore, in this study, polygalacturonase (PGase) produced
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Investigation of lentil flour utilization for the production of protease by Bacillus subtilis ZBP4 Biocatal. Biotransform. (IF 1.863) Pub Date : 2021-01-07 Ayşe Avcı; Merve Değirmen; Fikriye Alev Akçay
Abstract In the current study, use of cost effective nitrogen source in the production of protease was aimed by using Bacillus subtilis ZBP4 which is an indigenous strain capable of producing various enzymes and industrially important metabolites. Lentil flour (LF) was used for this purpose, and enzyme production from LF was compared with some other nitrogen sources including casein, yeast extract
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Response surface methodology for optimization of Rhizopus stolonifer 1aNRC11 mutant F whole-cell lipase production as a biocatalyst for methanolysis of waste frying oil Biocatal. Biotransform. (IF 1.863) Pub Date : 2021-01-07 Sayeda S. Mohamed; Hanan M. Ahmed; Magda A. El-Bendary; Maysa E. Moharam; Hala A. Amin
Abstract Biodiesel, fatty acid methyl ester (FAME), is an environmentally friendly alternative fuel produced by transesterification of renewable lipid feedstocks for diesel engines. Methanolysis of waste frying oil (WFO) was catalyzed by Rhizopus stolonifer 1aNRC11 mutant F whole-cell lipase that produced under solid state fermentation (SSF) using wheat bran (WB) and black cumin meal as the main substrate
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Stabilization of multimeric nitrilase via different immobilization techniques for hydrolysis of acrylonitrile to acrylic acid Biocatal. Biotransform. (IF 1.863) Pub Date : 2021-01-07 Ali Toprak; S. Seyhan Tükel; Deniz Yildirim
(2021). Stabilization of multimeric nitrilase via different immobilization techniques for hydrolysis of acrylonitrile to acrylic acid. Biocatalysis and Biotransformation. Ahead of Print.
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Inulinase immobilisation in PAA/PEG composite for efficient fructooligosaccharides production Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-12-26 Mishela Temkov; Darko Dimitrovski; Elena Velickova; Albert Krastanov
Abstract Inulinase was immobilised by entrapment method in polyacrylamide/polyethylene glycol composite and evaluated for its efficiency for short-chain fructooligosaccharides (3–6 degrees of polymerisation) production in batch hydrolysis system. Aqueous two-phase polymerisation technique was used to synthesise the composite, where aqueous polyethylene glycol 1000 containing the enzyme was used as
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Development of a process for the enhanced enzymatic digestibility of solid waste from tofu to yield fermentable biosugars Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-12-26 Qian Xu; Jiawei Wu; Lihua Zou; Jia Ouyang; Zhaojuan Zheng
Abstract Tofu solid waste (TSW), an abundant agricultural waste product from the soy industry, can be used as an excellent source of renewable biomass by the action of microbes, indicating a better utilization prospect as opposed to its disposal in landfills or being incinerated. In this study, the composition of TSW was investigated. The waste material was used to produce lactic acid by enzymatic
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Nadp+-dependent formate dehydrogenase: a review Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-12-23 Saadet Alpdagtas; Barış Binay
Abstract NADPH-dependent oxidoreductases are crucial biocatalysts for the industrial production of chiral compounds. For in situ recycling of required expensive cofactors in this biosynthetic process, NAD(P)+-dependent formate dehydrogenases (FDHs) are wanted to be employed as redox biocatalysts due to their greener and process friendly nature. However, their utilization is limited by their undesired
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Investigation new positions for catalytic activity of Chaetomium thermophilum and Ceriporiopsis subvermispora formate dehydrogenases Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-12-23 Kübra Koçdemir; Fatma Şen; Yasin Adem Wedajo; Muazzez Çağla Bilgici; Mustafa Bayram; İlke Selçuk; Berin Yılmazer; Mehmet Mervan Çakar; Elif Sibel Aslan; Barış Binay
Abstract NAD+-dependent formate dehydrogenases (FDHs, E.C 1.2.1.2) catalyse the reversible reaction of CO2 to formate ion (HCOO−) and reduces NAD+ molecule to NADH. Previously described FDHs from Chaetomium thermophilum (CtFDH) and Ceriporiopsis subvermispora (CsFDH) are active against formate and HCO3 –. In this study, we examined the functional effects of active site mutations in Ct and Cs NAD+-dependent
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Dynamic action of keratinase on wool fibre tracked by FITC-labelled enzyme Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-12-18 Zhang-Jun Cao; Xiao-Xiao Song; Qian Zhu; Juan Wang; Run Gong; Yun-Long Zhang; Gang Wang
Abstract Due to pollution in the process of chemical treatments, wool textile industry has been remarkably changed to use eco-friendly reagents in preparatory and finishing operations. We previously isolated a wool-degrading bacterial strain (Pseudomonas sp.3096-4) from decomposed wool fabrics, which produces a new type of keratinase capable of degrading cuticle layers. In this study, using fluorescein
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Laccase treatment of phenolic compounds for bioethanol production and the impact of these compounds on yeast physiology Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-12-07 Karla V. Teymennet-Ramírez; Fernando Martínez-Morales; Carlos Muñoz-Garay; Brandt Bertrand; Daniel Morales-Guzmán; María R. Trejo-Hernández
Abstract Laccase treatment of phenolic compounds found in lignocellulosic hydrolysates is an alternative to reduce the growth inhibitory effect of these compounds on fermenting microorganisms. In order to determine the main factors that affect the efficiency of this biocatalytic approach, laccase oxidation of individual and mixtures of phenolic compounds was evaluated. Additionally, the effect of phenolic
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Purification and characterization of novel α-amylase from Anoxybacillus ayderensis FMB1 Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-12-04 Fatma Matpan Bekler; Kemal Güven; Reyhan Gül Güven
Abstract The thermophilic amylase producing Anoxybacillus ayderensis FMB1 was isolated from Sorgun Hot Spring in Turkey. In order to obtain improved production of the enzyme for cost-effective process, optimization of the process parameters is required. Optimum conditions for amylase production were determined using the traditional Submerged Fermentation (SmF) method. The maximum amylase activity was
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Reaction kinetic studies on the immobilized-lipase catalyzed enzymatic resolution of 1-phenylethanol transesterification with ethyl butyrate Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-12-02 Weican Wang; Ling Li; Xiaoda Wang; Ting Qiu; Jianhao Yang; Changshen Ye
Abstract The enzyme-catalyzed transesterification reaction is an important route to realize the resolution of racemic optically active secondary alcohols. However, this route suffers from the high environment and engineering risks due to the usage of vinyl ester as acyl donor. In this work, an alternative acyl donor, ethyl butyrate, was used because it is more environment-friendly and catalyst-friendly
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Bioactive peptides and proteases: characteristics, applications and the simultaneous production in solid-state fermentation Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-11-19 Paola Gouvêa Manfredini; Vítor Augusto Farina Cavanhi; Jorge Alberto Vieira Costa; Luciane Maria Colla
Abstract Bioactive peptides are compounds hydrolysed from an inactive parental protein that has beneficial effects on health, such as reduction of blood pressure, antioxidant activity, reduction of problems caused by diabetes, anticancer action, among others. These compounds can be obtained by the hydrolysis of proteins by enzymes, as the proteases, which are produced by microorganisms through solid-state
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Microbial transformation of dehydroepiandrosterone (DHEA) by some fungi Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-11-05 Kudret Yildirim; Ali Kuru; Semra Yılmazer Keskin; Sinan Ergin
Abstract In this work, biotransformations of dehydroepiandrosterone (DHEA) 1 by Ulocladium chartarum MRC 72584, Cladosporium sphaerospermum MRC 70266 and Cladosporium cladosporioides MRC 70282 have been reported. U. chartarum MRC 72584 mainly hydroxylated 1 at C-7α and C-7β, accompanied by a minor hydroxylation at C-4β, a minor epoxidation from the β-face and a minor oxidation at C-7 subsequent to
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Comparative detoxification of Remazol Rrilliant Blue R by free and immobilized laccase of Oudemansiella canarii Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-10-26 Thaís Marques Uber; Ana Júlia dos Reis Buzzo; Gidiane Scaratti; Suélen Maria Amorim; Cristiane Vieira Helm; Giselle Maria Maciel; Rosely Aparecida Peralta; Regina de Fátima Peralta Muniz Moreira; Adelar Bracht; Rosane Marina Peralta
Abstract A laccase from Oudemansiella canarii was immobilized using the crosslinked enzyme aggregate (CLEA) methodology and applied in the degradation of the anthraquinonic dye Remazol Brilliant Blue R (RBBR). The immobilized laccase was superior to the free laccase in both thermal and storage stabilities. Both immobilized and free laccase decolourized 100 mg/L RBBR within 24 h at 30 °C and pH 5.0
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Effects of cellulase enzyme in deinking of Solvent-Based inks from mixed office wastes Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-10-23 Mustafa Cicekler; Ahmet Tutus
Abstract The effects of cellulase enzymes (Trichoderma reesei) in flotation deinking of mixed office wastes (MOW) on the deinking efficiency, physical and optical properties of the papers were investigated. In order to determine the effects of cellulase, conventional chemical deinking was also performed and compared with enzymatic deinking. After deinking processes, the handsheets were produced to
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Production of enantiomerically enriched chiral carbinols using whole-cell biocatalyst Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-10-21 Yasemin Baydaş; Erbay Kalay; Engin Şahin
Abstract Biocatalytic asymmetric reduction of ketone is an efficient method for the production of chiral carbinols. The study indicates selective bioreduction of different ketones (1–8) to their respective (R)-alcohols (1a–8a) in low to high selectivity (0- >99%) with good yields (11–96%). In this work, whole-cell of Lactobacillus kefiri P2 catalysed enantioselective reduction of various prochiral
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Current production of bioherbicides: mechanisms of action and technical and scientific challenges to improve food and environmental security Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-10-14 Eduarda Roberta Bordin; Aline Frumi Camargo; Fábio Sptiza Stefanski; Thamarys Scapini; Charline Bonatto; Jessica Zanivan; Karina Preczeski; Tatiani Andressa Modkovski; Francisco Reichert Júnior; Altemir José Mossi; Gislaine Fongaro; Wanessa Algarte Ramsdorf; Helen Treichel
Abstract Modern agriculture is threatened by the possibility of nutrient losses, largely attributable the presence of competing weeds in these spaces. The challenge is to manage these weeds efficiently without harming the environment and the population. The inadequate use of a series of management techniques ends up causing resistance in weeds; therefore, we seek new forms of control. Among these are
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Chitosan-glutaraldehyde activated carrageenan-alginate beads for β-D-galactosidase covalent immobilisation Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-10-14 Marwa I. Wahba; Mohamed E. Hassan; Korany A. Ali
Abstract Chitosan (CS) and glutaraldehyde (GA) were exploited to activate carrageenan-alginate (Car-Alg) beads and enable their utilisation as covalent immobilisation matrices. The GA/CS activated Car-Alg beads were utilised to immobilise the Aspergillus oryzae β-D-galactosidase (β-gal). Initially, the beads gelling solution was optimised, and it was shown that the upmost amount of immobilised β-gal
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Synthesis of flavor compound ethyl hydrocinnamate by Yarrowia lipolytica lipases Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-10-05 Bartłomiej Zieniuk; Agata Fabiszewska; Małgorzata Wołoszynowska; Ewa Białecka-Florjańczyk
Abstract Two biocatalysts with high lipolytic activity were obtained – freeze-dried supernatant and freeze-dried biomass of Yarrowia lipolytica KKP 379. The biocatalysts were compared with Candida antarctica lipase B in the synthesis of ethyl hydrocinnamate – a flavour compound with a floral-honey aroma. Ester synthesis was completed after 2 h when 3 grams of freeze-dried biomass was used. Using smaller
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Optimization of lactose derivative hetero-oligosaccharides production using whey as the acceptor molecule by an active glucansucrase Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-09-29 Mohammad Kabli; Hümeyra İspirli; Mohammed Balubaid; Osman Taylan; Mustafa Tahsin Yılmaz; Enes Dertli
Abstract Glucansucrases are capable of synthesis of distinct oligosaccharides using different acceptor molecules and in this study lactose derivative hetero-oligosaccharides were shown to be produced by an active glucansucrase in its acceptor reaction with sucrose and whey as the donor-acceptor molecules, respectively. Following the monitoring of the reaction products by TLC analysis, the effects of
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Designing synthetic microbial communities for effectual bioremediation: A review Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-09-02 Babita Sharma; Pratyoosh Shukla
Bioremediation is a better alternative and widely accepted approach used for efficient degradation of environmental pollutants released from industries, urban and agricultural activities due to its eco-friendly nature. Systems biology helps in the identification of new genes, proteins, metabolites, and metabolic pathways involved in bioremediation. Such information can be used for designing synthetic
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Glycoside hydrolase family 68 gene of halophilic archaeon Halalkalicoccus jeotgali B3T codes for an inulosucrase enzyme Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-09-08 Komal Ghauri; Hazrat Ali; Nayla Munawar; Muhammad Afzal Ghauri; Munir Ahmad Anwar
Fructose polysaccharides and oligosaccharides are gaining strong importance due to their beneficial effects on human health and their applications in the food, feed and pharmaceutical industries. Fructan synthesis has been characterised in a wide variety of bacteria and plants. Metagenomic DNA sequence data suggests that several archaeal strains also contain fructosyltransferase (ftf) genes in their
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A study on enhanced production of 3-demethylated colchicine by a novel strain of Bacillus endophyticus isolated from rhizospheric soils of Gloriosa superba Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-08-18 Reena Das; Vasantharaja A; Prithika Udayakumar; Rama Vaidyanathan
A native strain of Bacillus endophyticus, PC was found to be effective in demethylation of colchicine. The strain was isolated from rhizospheric soils of Gloriosa superba from Erode, Tamilnadu, and was grown in optimised conditions of carbon and nitrogen. To intensify the demethylation, the strain was enriched and grown for 15 generations starting from low (0.5 mg/ml) to very high (10 mg/ml) concentrations
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Characterization of a recombinant bile salt hydrolase (BSH) from Bifidobacterium bifidum for its glycine-conjugated bile salts specificity Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-08-11 Chou Li; Qingzhi Ji; Tongyao He; Yingying Liu; Yunqing Ma
Deconjugation of bile salts in vivo not only promotes the cholesterol metabolism of the host, but also provides cellular carbon, nitrogen and sulphur for some probiotics. In this study, the gene of BSH from Bifidobacterium bifidum (ATCC 29521) was amplified and expressed using glutathione S-transferase (GST) Gene Fusion System. The enzyme was purified in a mild conditions without denaturation and renaturation
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Regio- and stereoselective hydroxylation of testosterone by cytochrome P450 from Streptomyces griseus ATCC 13273 Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-08-03 Jinqian Zhu; Chen Shen; Wanli Zhao; Xiufeng Liu; Jihua Liu; Boyang Yu
16α-Hydroxylated steroids are important precursors in the synthesis of high value steroidal drugs such as glucocorticoids in the pharmaceutical industry. In this study, an innovative biotechnological method based on enzymatic catalysis was presented for the high-efficient production of 16α-hydroxyl-testosterone. CYP154C, a cytochrome P450 from Streptomyces griseus ATCC 13273, was identified to carry
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Development of a biocatalytic cascade for synthesis of 2-oxo-4-(hydroxymethylphosphinyl) butyric acid in one pot Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-07-25 Jianmiao Xu; Kai Zhang; Huiting Cao; Heng Li; Feng Cheng; Chenghao Cao; Ya-Ping Xue; Yu-Guo Zheng
2-Oxo-4-(hydroxymethylphosphinyl) butyric acid (PPO) is an important precursor compound for the broad-spectrum herbicide l-glufosinate (L-PPT). In this study, the gene of d-amino acid oxidase (DAAO) was cloned and expressed in Escherichia coli. By coupling exogenous catalase (CAT), a biocatalytic cascade was constructed for synthesis of PPO in one pot. The bioprocess was optimized on a 300 mL scale
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Exploiting benign ionic liquids to effectively synthesize chiral intermediate of NK-1 receptor antagonists catalysed by Trichoderma asperellum cells Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-07-25 Li Jun; Qian Feng; Pu Wang
This study aims to exploit an ionic liquid (IL)-containing medium for efficient production of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol ((R)-BTPE) from 3,5-bis(trifluoromethyl) acetophenone (3,5-BTAP) catalysed by whole cells of Trichoderma asperellum ZJPH0810. A new type of quaternary ammonium-based ILs (QAILs) was investigated and compared with more conventional pyridinium or imidazolium-based
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Preparation and characterization of amino and carboxyl functionalized core-shell Fe3O4/SiO2 for L-asparaginase immobilization: A comparison study Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-05-19 Samir Abbas Ali Noma; Ahmet Ulu; Suleyman Koytepe; Burhan Ateş
Magnetic nanoparticles are well known as facile and effective support for enzyme immobilization since they have a high surface area, large surface-to-volume ratio, easy separation, a fast and high enzyme loading. This study aims to provide insights on whether acidic or basic modified particles are more effective for L-asparaginase (ASNase) immobilization. Therefore, amino (Fe3O4/SiO2/NH2) and carboxyl-functionalized
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Characterisation of free and immobilised laccases from Ganoderma lucidum: application on bisphenol a degradation Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-07-13 Tatiane Brugnari; Alex Graça Contato; Marita Gimenez Pereira; Emanuelle Neiverth de Freitas; Gisele Adriana Bubna; Guilherme Mauro Aranha; Adelar Bracht; Maria de Lourdes Teixeira de Moraes Polizeli; Rosane Marina Peralta
Laccases (EC. 1.10.3.2) have a broad range of industrial applications. In order to overcome problems associated with loss of activity, storage and reusability of the free enzyme, a laccase from Ganoderma lucidum was immobilised successfully on monoaminoethyl-N-aminoethyl (MANAE)-agarose with high immobilisation yield and high activity recovery. The immobilisation improved significantly the thermal
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Biotransformation of longifolene by Penicillium europium Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-07-10 Aejaz A. Khan; S. M. Shakeel Iqubal; Ibrahim Ahmed Shaikh; Francois Niyongabo Niyonzima; Veena S. More; Uday M. Muddapur; R. S. Bennur; Sunil S. More
A fungal species was screened from a forest soil by enrichment agar plating method with longifolene as only carbon source. It was identified as Penicillium europium. The isolated fungus transformed the longifolene into various metabolites, of which 12 were isolated in pure form. The characterisation of these transformed compounds was carried out by physicochemical methods involving spectral studies
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Optimisation of the bioreduction process of carbonyl compounds promoted by seeds of glossy privet (Ligustrum lucidum - Oleaceae) and its application to the synthesis of key intermediates Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-07-09 Daniela L. Bordón; Ana M. Vázquez; María F. Decarlini; Gabriela I. Demmel; Laura I. Rossi; Mario L. Aimar
The fruits of Ligustrum lucidum were used as an efficient biological reducer of carbonyl compounds, and trials were carried out to optimise the reductive process using acetophenone as the model substrate. In order to try to eliminate the dependence on the seasonal production of the fruits, a study was carried out to find ways to conserve them. The results reported here show that it is possible to store
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Optimisation, scope and advantages of the synthesis of chiral phenylethanols using whole seeds of Bauhinia variegata L. (Fabaceae) as a new and stereoselective bio-reducer of carbonyl compounds Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-07-07 Gabriela I. Demmel; Daniela L. Bordón; Ana M. Vázquez; María F. Decarlini; Gustavo M. Ruiz; Juan J. Cantero; Laura I. Rossi; Mario L. Aimar
With the aim of finding new methods for environmentally friendly synthesis of chiral phenylethanols, a screening was carried out to identify seeds that could be used as a biocatalyst capable of reducing stereoselectively prochiral ketones. As a result, seeds of Bauhinia variegata L. (Fabaceae) were identified as being an efficient and stereoselective biological reducer of acetophenone to produce (S)-1-phenylethanol
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Polyhydroxyalkanoate biosynthesis and characterization from optimized medium utilizing distillery effluent using Bacillus endophyticus MTCC 9021: a statistical approach Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-07-07 Kumaresan Priyanka; Mridul Umesh; Basheer Thazeem; Kathirvel Preethi
Use of expensive raw material and inefficient extraction method has limited the production of biopolymers at pilot scale compared to commercial plastics. Hence, this research work spotlights on production of polyhydroxyalkanoate (PHA) using Bacillus endophyticus MTCC 9021 in different dilutions of untreated distillery effluent (10–25%) among which 10% dilutions exhibited maximum PHA production. Statistical
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Improving the antioxidant and antidiabetic properties of common bean proteins by enzymatic hydrolysis using a blend of proteases Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-07-06 André Ohara; Victor Granato Cason; Tânia Goia Nishide; Francielle Miranda de Matos; Ruann Janser Soares de Castro
Bioactive peptides are fragments of polypeptide chains with specific sequence of amino acids that can promote positive effects on human health. A potential substrate to obtaining these compounds is the common bean (Phaseolus vulgaris L. cv Carioca) due to its high protein content. In this sense, the objective of this study was to evaluate the antioxidant and antidiabetic properties of common bean protein
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Biotransformation of bovine tannery fleshing into utilizable product with multifunctionalities Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-07-01 Basheer Thazeem; Mridul Umesh; Vellingiri Manon Mani; Goldy Primo Beryl; Kathirvel Preethi
Leather industries aim at the transformation of putrescible animal raw hides and skins into a stable non-putrescible material (leather), ultimately resulting in solid wastes generation. Storage of limed bovine fleshing, a predominant proteinaceous solid waste material from pre-tanning processes is highly discouraged due to their putrescible nature. Diversely, bio-conversion of solid waste into a saleable
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Glucansucrases from lactic acid bacteria as biocatalysts for multi-ring catechol glucosylation Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-06-26 Johannes Nolte; Alexander Kempa; Matthias Hochgürtel; Ulrich Schörken
Catechins are the major group of bioactive flavanols in green tea and cacao. 17 glucansucrase-active strains were identified from a set of 41 lactic acid bacteria, which were able to glucosylate (+)-catechin in a non-natural acceptor reaction. In total cell free extracts of 12 Leuconostoc and 5 Weissella strains were active on catechin and also 8 cell fractions exhibited catechin glucosylation activity
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Characterisation of a thermostable and proteolysis resistant phytase from Penicillium polonicum MF82 associated with the marine sponge Phorbas sp. Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-06-26 Saban Orcun Kalkan; Elif Bozcal; Elif Esin Hames Tuna; Atac Uzel
Phytases are widely used in human and animal nutrition, aquaculture, soil amendment, and in the production of lower myo-inositol phosphates for clinical purposes. Some of these applications, especially feed industry require robust enzymes. Since the marine environments are less studied compared to terrestrial environments, we evaluated the extracellular phytase activity of 110 marine derived filamentous
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Supplementation of watermelon peels as an enhancer of lipase and esterase production by Yarrowia lipolytica in solid-state fermentation and their potential use as biocatalysts in poly(ethylene terephthalate) (PET) depolymerization reactions Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-06-26 Julio Cesar S. Sales; Aline M. de Castro; Bernardo D. Ribeiro; Maria Alice Z. Coelho
Yarrowia lipolytica is a yeast that presents high biotechnological potential due to its ability to produce many metabolites, among them lipases and esterases, which are important industrial biocatalysts. Since Brazil is an agroindustrial country, it generates an enormous diversity of residues or byproducts that can be used as a platform for biomolecules production. This work aims to evaluate lipase
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Biotransformation of acrylamide to acrylic acid carried through acrylamidase enzyme synthesized from whole cells of Bacillus tequilensis (BITNR004) Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-06-22 Riddhi Prabha; Vinod Kumar Nigam
In this study, an amidase-synthesizing and inducible thermotolerant bacterium, Bacillus tequilensis (BITNR004), was investigated for the conversion of acrylamide to acrylic acid, which was subsequently confirmed using various analytical tools. The enzyme, acrylamidase, was found to be intracellular in nature with optimum activity at 50 °C. Further, the whole-cell biocatalyst had a biotransformation
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Bio-catalytic asymmetric synthesis of β-adrenergic receptor blocker precursor: (R)-2-bromo-1-(naphthalen-2-yl)ethanol Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-05-22 Volkan Taşdemir; Erbay Kalay; Enes Dertli; Engin Şahin
Aromatic α-halohydrins, particularly 2-haloethanols as significant precursor of drugs, can easily be converted to chiral β-adrenergic receptor blockers. Eight strains of Lactobacillus curvatus were tested as biocatalysts for asymmetric reduction of 2-bromo-1-(naphthalen-2-yl)ethanone 1 to 2-bromo-1- (naphthalen-2-yl) ethanol 2. The parameters of the bioreduction were optimized using L. curvatus N4
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CALB-catalyzed kinetic resolution of (RS)-3-benzoylthio-2-methylpropyl azolides: kinetic and thermodynamic analysis Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-04-27 Chen-Hao Kuo; Bo-Son Kou; Shau-Wei Tsai
Zofenopril as an ACE inhibitor expired recently was found to have a favourable safety profile in comparison with other ACE inhibitors in treating high blood pressure, congestive heart failure, and acute myocardial infarction. It can be synthesised from the key building blocks of (S)-3-benzoylthio-2-methylpropanoic acid and (4S)-phenylthio-L-proline. In this report, an efficient hydrolytic resolution
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Enzymatic synthesis of a catecholic polyphenol product with excellent antioxidant activity Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-04-25 Yi-Xiong Wei; Xiao-Feng Lü; Hui Cheng; Jia-Yi Luo; Yong Zou; Zhen Yang
Polyphenols, especially catecholic stilbene derivatives, have attracted much attention due to the huge pharmacological effects and promising health benefits. However, their chemical synthesis via regioselective ortho-hydroxylation on aromatic rings is highly challenging. In this study, 3′-hydroxypterostilbene (HPS) is taken as a model product due to its strong potential as an antitumor agent. One-step
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Non-isothermal kinetic modelling of potassium indigo-trisulfonate dye discolouration by Horseradish peroxidase Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-04-22 Flávia Nunes Costa; Diego Alex Mayer; Alexsandra Valério; Janaina de Souza Lima; Débora de Oliveira; Antônio Augusto Ulson de Souza
Textile industries account for two-thirds of the total dyestuff market been responsible for the production of a large volume of effluent with unfixed dye. Aromatic dyes as potassium indigo-trisulfonate dye (PIT) are characterized as a chemically stable and complex molecule with high heat and light stability and with high toxicity even at low concentration. In order to evaluate an environmentally friendly
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Atrazine, desethylatrazine (DEA) and desisopropylatrazine (DIA) degradation by Pleurotus ostreatus INCQS 40310 Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-04-22 Raquel de Oliveira Lopes; Patrícia Maia Pereira; Aline Ramalho Brandão Pereira; Keysson Vieira Fernandes; Julia Finamor Carvalho; Alexandre da Silva de França; Richard Hemmi Valente; Manuela da Silva; Viridiana S. Ferreira-Leitão
Atrazine is the most common herbicide applied in crops of economic relevance, such as sugar cane, soybean, and corn. Atrazine and its derivatives desethylatrazine (DEA) and desisopropylatrazine (DIA) are toxic to the environment, affecting animal and human health. Thus, this study aimed to evaluate the degradation of atrazine and its derivatives by the fungus Pleurotus ostreatus INCQS 40310, as well
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Plant proteases from Carica papaya and Vasconcellea quercifolia with potential application for a cleaner processing in tanneries Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-04-17 María Eugenia Errasti; María José Torres; Julio Ricardo Mercerat; Néstor Oscar Caffini; Laura María Isabel López
In the leather industry, proteases can be used as tools to achieve cleaner technology. In the present study, proteolytic preparations obtained from the latex of Carica papaya (Cp) and Vasconcellea quercifolia (Vq) were assayed as dehairing agents for leather processing. Both Cp and Vq showed activity against substrates representative of collagen, keratin, elastin, and epidermis in a range of moderate
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Secretome analysis of thermophilic mould Myceliophthora thermophila cultivated on rice straw and hydrolysis of lignocellulosic biomass for bioethanol production Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-04-16 Anju Bala; Anu; Alokika; Anil Kumar; Sanjeev Kumar; Davender Singh; Bijender Singh
Myceliophthora thermophila encodes for large number of carbohydrate-active enzymes (CAZymes) involved in lignocellulosic biomass degradation. The mould was grown on rice straw in solid state fermentation at pH 5.0 and 45 °C that produced high levels of cellulolytic and xylanolytic enzymes i.e. 2218.12, 515.23, 478.23, 13.34 U/g DMR for xylanase, CMCase, FPase and β-glucosidase, respectively. The secretome
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Screening, partial purification and characterization of the hyper-thermophilic lipase produced by a new isolate of Bacillus subtilis LP2 Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-04-11 Gulhan Yasar; Unzile Guven Gulhan; Elif Guduk; Fatih Aktas
(2020). Screening, partial purification and characterization of the hyper-thermophilic lipase produced by a new isolate of Bacillus subtilis LP2. Biocatalysis and Biotransformation: Vol. 38, No. 5, pp. 367-375.
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Estimation of initial rate from discontinuous progress data Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-04-02 Peter J. Halling
(2020). Estimation of initial rate from discontinuous progress data. Biocatalysis and Biotransformation: Vol. 38, No. 5, pp. 325-342.
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Adding value to a recalcitrant and problematic waste: the use of dog hair for fungal keratinolytic protease production Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-04-02 Thaiane Prolo; Simone Cristine Izidoro; Vanderlei Aparecido de Lima; Guilherme Arielo Rodrigues Maia; Adriana Knob
The demand for keratinolytic proteases has increased in recent years, in view of their applications in the feed, detergent, fertilizer, leather and textile industries. Recently, studies have focussed on exploration of new and inexpensive carbon sources for their production. Among keratin wastes, dog hair presents no utility and is an environmental concern. In this study, we evaluate the feasibility
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The headspace-GC/MS: Alternative methodology employed in the bioreduction of (4S)-(+)-carvone mediated by human skin fungus Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-03-31 Rogério Aparecido Minini dos Santos; Adriano Valim Reis; Eduardo Jorge Pilau; Carla Porto; José Eduardo Gonçalves; Arildo José Braz de Oliveira; Regina Aparecida Correia Gonçalves
The development of more efficient and environmentally friendly analytical methods represents a current focus for the fine chemical industry. In particular, microscale methodologies that are free of solvents/reagents. The headspace-GC/MS (HS-GC) methodology was employed in this study as a tool for monitoring a biocatalysed reaction of (4S)-(+)-carvone using Phoma sp., a filamentous fungus from human
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Effect of hydrophobicity degree of polymer particles on lipase immobilization and on biocatalyst performance Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-03-15 Martina C. C. Pinto; Stefanni S. Everton; Leilane C. M. Cirilo; Thalita N. de Melo; Eliane P. Cipolatti; Evelin A. Manoel; José Carlos Pinto; Denise M. G. Freire
Many studies describe the advantages of using hydrophobic particles on lipase immobilisation. However, many of these works neglect the effect of other variables of the supports, such as specific area and porosity, on the biocatalyst performance, and do not evaluate the influence of the hydrophobicity level of the particles on the biocatalysts’ activity as a single variable. Thus, the focus of the present
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A practical approach to obtain high yield lipase-mediated synthesis of octyl caprylate with Novozym 435 Biocatal. Biotransform. (IF 1.863) Pub Date : 2020-03-11 Ronaldo Rodrigues de Sousa; Leonardo Vitor Belo Pazutti; Gabriel Zamith Leal Dalmaso; Diane Frauches Siqueira; Ayla Sant’Ana da Silva; Viridiana Santana Ferreira-Leitão
This study evaluated coupled effects of molar ratio of substrates and enzyme loading in a solvent-free system using a simple mathematical approach to obtain high conversions on octyl caprylate synthesis with Novozym 435. When molar ratios of caprylic acid to n-octanol (1:1 and 1:3) were evaluated with enzyme loadings of 1% to 4% (wt/wt acid), an interdependence between the masses of reagents and the
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Immobilization of Rhizomucor miehei lipase onto montmorillonite K-10 and polyvinyl alcohol gel Biocatal. Biotransform. (IF 1.863) Pub Date : 2019-12-16 Ece Ozdemir Babavatan; Deniz Yildirim; Aysegul Peksel; Baris Binay
Immobilization of enzymes from different sources on various supports in designed systems increases enzymes’ stability by protecting the active site of it from undesired effect of reaction environment. Also, immobilization decreases the cost of separation and facilities the reuse of the enzymes. Therefore, the design of new immobilization enzyme preparations has been an inevitable area of modern biotechnology
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Some biochemical, catalytic, thermodynamic and kinetic properties of purified fructosyltransferase from wild and improved mutant-type Aureobasidium pullulans NAC8 Biocatal. Biotransform. (IF 1.863) Pub Date : 2019-10-01 Adedeji N. Ademakinwa; Femi K. Agboola
A low molecular weight intracellular and extracellular fructosyltransferase was purified from the wild (Wt) and improved mutant-type (Mt) Aureobasidium pullulans and its characteristics and thermodynamic properties determined. The Wt had been previously genetically modified by chemical mutagenesis. The purified fructosyltransferases from the Wt and Mt had subunit molecular weights between 13.4 and
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Aggregation properties of cold-active lipase produced by a psychrotolerant strain of Pseudomonas palleroniana (GBPI_508) Biocatal. Biotransform. (IF 1.863) Pub Date : 2019-09-24 Rahul Jain; Neha Pandey; Anita Pandey
The present study aims to exploit microbial potential from colder region to produce lipase enzyme stable at low temperatures. A newly isolated bacterium GBPI_508 from Himalayan environment, was investigated for the production of cold-active lipase emphasizing on its aggregation properties. Plate based assays followed by quantitative production of enzyme was estimated under different culture conditions
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Kinetically controlled acylation of 6-APA catalyzed by penicillin acylase from Streptomyces lavendulae: effect of reaction conditions in the enzymatic synthesis of penicillin V Biocatal. Biotransform. (IF 1.863) Pub Date : 2019-08-14 Daniel Hormigo; María Teresa López-Conejo; Lara Serrano-Aguirre; Alberto García-Martín; Ana Saborido; Isabel de la Mata; Miguel Arroyo
Enzymatic synthesis of penicillin V (penV) by acylation of 6-aminopenicillanic acid (6-APA) was carried out using methyl phenoxyacetate (MPOA) as activated acyl donor and soluble penicillin acylase from Streptomyces lavendulae (SlPVA) as biocatalyst. The effect of different reaction conditions on penV synthesis was investigated, such as enzyme concentration, pH, molar ratio of 6-APA to MPOA, as well
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Cyanide-free synthesis of an aromatic nitrile from a biorenewable-based aldoxime: Development and application of a recombinant aldoxime dehydratase as a biocatalyst Biocatal. Biotransform. (IF 1.863) Pub Date : 2019-05-23 Ji Eun Choi, Suguru Shinoda, Risa Inoue, Daijun Zheng, Harald Gröger, Yasuhisa Asano
An Escherichia coli host microorganism was used for the production of the aldoxime dehydratase from Rhodococcus sp. YH3-3 (OxdYH3-3), which showed good activity toward aromatic aldoximes. Biotransformation of aromatic aldoximes to nitriles was studied using this recombinant biocatalyst OxdYH3-3, exemplified in particular for the synthesis of biorenewable-based 2-furonitrile. The gene encoding for the
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Enantioselective resolution of (±)-1-phenylethyl acetate by extracellular proteases from deep-sea bacterium Bacillus sp. DL-2 Biocatal. Biotransform. (IF 1.863) Pub Date : 2019-05-23 Lu Dong, Yongkai Xu, Yun Zhang, Aijun Sun, Yunfeng Hu
Chiral 1-phenylethanol and its ester derivative are important chiral chemicals in diverse industries and the preparation of those optically pure enantiomers is of great importance. One bacterium, Bacillus sp. DL-2, whose extracellular proteases could efficiently asymmetrically hydrolyse (±)-1-phenylethyl acetate, was isolated from the deep sea of the Western Pacific. After the immobilization of extracellular
更新日期:2019-05-23