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CircPlekha7 plays an anti-fibrotic role in intrauterine adhesions by modulating endometrial stromal cell proliferation and apoptosis. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Wei Xie,Min He,Yuhuan Liu,Xiaowu Huang,Dongmei Song,Yu Xiao
Circular RNA (circRNA) plays a key role in the development and progression of several diseases; however, its role in intrauterine adhesions (IUAs) is not well understood. This study aims to investigate the expression profiles and potential role of circRNA in IUA. RNA-sequencing was performed to screen for abnormally expressed circRNAs in TGF-β1-induced IUA endometrial stromal cell (ESC) model (IUA
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Locus-specific analysis of DNA methylation patterns in cloned and in vitro fertilized porcine embryos. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Weihua Xu,Hongyi Li,Mao Zhang,Junsong Shi,Zhengchao Wang
Porcine somatic cell nuclear transfer (SCNT) is currently inefficient, as 1–3.95% of reconstructed embryos survive to term; inadequate or erroneous epigenetic reprogramming of the specialized donor somatic nucleus could be a primary reason. Therefore, a locus-specific analysis of DNA methylation dynamics in embryogenesis and the DNA methylation status of gametes and donor cells used for SCNT were conducted
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Expression and functions of cluster of differentiation 9 and 81 in rat mammary epithelial cells. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Kotaro Horiguchi,Saishu Yoshida,Takehiro Tsukada,Takashi Nakakura,Ken Fujiwara,Rumi Hasegawa,Shu Takigami,Shunji Ohsako
Cluster of differentiation (CD) 9 and CD81 are closely-related members of the tetraspanin family that consist of four-transmembrane domain proteins. Cd9 and Cd81 are highly expressed in breast cancer cells; however, their expression in healthy mammary glands is unclear. In this study, we performed quantitative real-time PCR to analyze the expression levels of Cd9 and Cd81. Histological techniques were
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Effects of human chorionic gonadotropin and intravaginal progesterone device treatment after artificial inseminations on the reproductive performance of normal and repeat breeder lactating dairy cows J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Taiki IZUMI; Ryotaro MIURA; Natsumi SOBU; Akiya HIRASE; Osamu YONEYAMA; Yoh-ichi MIYAKE; Shingo HANEDA; Motozumi MATSUI
We examined the effects of human chorionic gonadotropin (hCG) treatment on Day 5 (Day 0 = day of artificial insemination: AI) and intravaginal progesterone device (IVPD) treatment from Day 5 to 19 on the conception and detection rates of return to estrus (re-estrus) in lactating dairy cows. A total of 306 cows from a commercial dairy farm were divided into the following three groups on Day 5: non-treatment
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Unique morphological characteristics in the ovary of cotton rat (Sigmodon hispidus) J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Md. Rashedul ISLAM; Osamu ICHII; Teppei NAKAMURA; Takao IRIE; Md. Abdul MASUM; Marina HOSOTANI; Yuki OTANI; Yaser Hosny Ali ELEWA; Yasuhiro KON
Cotton rats (Sigmodon hispidus, CRs) are commonly used as animal models in biomedical research. However, the reproductive characteristics and ovarian development in the CRs has not been widely investigated. We have previously shown that female CRs, in particular, show several unique phenotypes associated with the urogenital system, such as chronic kidney disease and pyometra. Our investigation revealed
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Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Mitsuru Kobayashi,Jun Ito,Koumei Shirasuna,Takehito Kuwayama,Hisataka Iwata
We examined the effect of ploidy on mitochondrial DNA (mtDNA) copy number in embryos and the amount of cell-free mitochondrial and nucleic DNA content (cf-mtDNA and cf-nDNA) in spent culture medium (SCM). Oocytes collected from the ovaries were matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to produce haploid or diploid embryos (H-group
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Effect of aging on mitochondria and metabolism of bovine granulosa cells. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Shuta Nagata,Kaoru Tatematsu,Kazuki Kansaku,Yuki Inoue,Mitsuru Kobayashi,Koumei Shirasuna,Hisataka Iwata
This study investigated the effect of aging on mitochondria in granulosa cells (GCs) collected from the antral follicles of young and aged cows (25–50 months and over 140 months in age, respectively). When GCs were cultured under 20% O2 for 4 days, mitochondrial DNA copy number (Mt-number), determined by real-time PCR, increased throughout the culture period, and the extent of increase was greater
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Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Yan-xia PENG; Cheng-Zhen CHEN; Dan LUO; Wen-jie YU; Sheng-peng LI; Yue XIAO; Bao YUAN; Shuang LIANG; Xue-rui YAO; Nam-Hyung KIM; Hao JIANG; Jia-Bao ZHANG
Carnosic acid (CA), a natural catechol rosin diterpene, is used as an additive in animal feeds and human foods. However, the effects of CA on mammalian reproductive processes, especially early embryonic development, are unclear. In this study, we added CA to parthenogenetically activated porcine embryos in an in vitro culture medium to explore the influence of CA on apoptosis, proliferation, blastocyst
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Monitoring follicular dynamics to determine estrus type and timing of ovulation induction in captive brown bears (Ursus arctos) J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Yoshiko TORII; Naoya MATSUMOTO; Hideyuki SAKAMOTO; Masashi NAGANO; Seiji KATAGIRI; Yojiro YANAGAWA
It is important to understand ovarian physiology when developing an artificial insemination (AI) protocol. Brown bears (Ursus arctos) have a breeding season from May to July, although the type of estrus (polyestrus or monoestrus) is still contested. The present study aimed to define the ovarian dynamics, including follicular waves and ovulatory follicle size, and estrus type in brown bears. Six brown
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Estrogen induces phosphorylation of prolactin through p21-activated kinase 2 activation in the mouse pituitary gland J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Kazunori MOROHOSHI; Yuichiro KOMATANI; Toshio HARIGAYA
A phosphorylated prolactin is a kind of modified prolactin, which is produced through phosphorylation of native prolactin (PRL) by p21-activated kinase 2 (PAK2) in secretory granules in lactotrophs. Phosphorylated prolactin is involved in the regulation of the estrous cycle and in apoptosis in cancer cells, which seem to have important physiological and pathological roles, respectively. In previous
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Mating-induced increase in Kiss1 mRNA expression in the anteroventral periventricular nucleus prior to an increase in LH and testosterone release in male rats J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Youki WATANABE; Kana IKEGAMI; Sho NAKAMURA; Yoshihisa UENOYAMA; Hitoshi OZAWA; Kei-ichiro MAEDA; Hiroko TSUKAMURA; Naoko INOUE
Kisspeptin has an indispensable role in gonadotropin-releasing hormone/gonadotropin secretion in mammals. In rodents, kisspeptin neurons are located in distinct brain regions, namely the anteroventral periventricular nucleus-periventricular nucleus continuum (AVPV/PeN), arcuate nucleus (ARC), and medial amygdala (MeA). Among them, the physiological role of AVPV/PeN kisspeptin neurons in males has not
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Inhibitory effects of long-term repeated treatments of a sustainable GnRH antagonist, degarelix acetate, on caprine testicular functions. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Noritoshi Kawate,Ryota Kanuki,M A Hannan,W W P N Weerakoon
We investigated the effects of long-term repeated treatments with a sustainable gonadotropin-releasing hormone (GnRH) antagonist, degarelix acetate, on testicular hormonal secretion, size, ultrasound images, histology and spermatogenesis in goats to assess its efficacy as a chemical castration method. Male Shiba goats (3–6 months of age) were treated subcutaneously with degarelix acetate every 4 weeks
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Efficacy of a single blood anti-Müllerian hormone (AMH) concentration measurement for the selection of Japanese Black heifer embryo donors in herd breeding programs J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Yasuo FUSHIMI; Hiroaki OKAWA; Danielle MONNIAUX; Mitsuhiro TAKAGI
We evaluated the relationship between plasma anti-Müllerian hormone (AMH) concentrations in Japanese Black (JB) heifers at 7−10 months of age and the number of embryos recovered after superovulation treatment in selected ovum pick-up donors, concomitantly with changes in their AMH concentrations before and after parturition. Plasma AMH concentrations in heifers were positively correlated with the total
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Successful activation of rat T lymphocytes by sperm specific antigens in vitro J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-22 Junko NOGUCHI; Mitsumi IKEDA; Kazuhiro KIKUCHI; Thanh Quang DANG-NGUYEN; Megumi KASASHIMA; Ken-ichiro TATEMATSU; Hideki SEZUTSU; Tadashi FURUSAWA
Autoimmune orchitis is a condition related to cellular immunity. A disease model involving transfer of T lymphocytes activated by known antigens would be useful for defining pathogenical molecules. Since no method for activating rat T cells using specific antigens is available, we started the study to develop the method. T cells were collected from draining lymph nodes of immunized rats, then co-cultured
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Expression and localization of alpha-tubulin N-acetyltransferase 1 in the reproductive system of male mice J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-26 Rin YANAI; Yudai YAMASHITA; Kohei UMEZU; Yuuki HIRADATE; Kenshiro HARA; Kentaro TANEMURA
The structure of microtubules is essential for the fertilizing ability of spermatozoa. Acetylation of α-tubulin plays an important role in flagellar elongation and spermatozoa motility. Previous reports have suggested that alpha-tubulin N-acetyltransferase 1 (ATAT1) is the main acetyltransferase involved in the acetylation of α-tubulin. Although ATAT1 is reported to express in the testis, no information
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Corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the TNF-α system J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-12 Ying-Qi ZHAO; Ren-Ren CHEN; Qiao-Qiao KONG; Jin-Song AN; Xin-Yue ZHAO; Shuai GONG; Hong-Jie YUAN; Jing-He TAN
It has been reported in recent studies that restraint stress on pregnant mice during the preimplantation stage elevated corticotrophin-releasing hormone (CRH) and glucocorticoid levels in the serum and oviducts; furthermore, CRH and corticosterone (CORT) impacted preimplantation embryos indirectly by triggering the apoptosis of oviductal epithelial cells (OECs) through activation of the Fas system
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Predicting the start of calving in Japanese Black cattle using camera image analysis J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-08 Hisashi NABENISHI; Natsuko NEGISHI; Atusi YAMAZAKI
This study assessed the feasibility of using camera image analysis to detect behavioral changes as an indicator of the onset of calving in Japanese Black cattle. Thirty-five pregnant cattle individually housed in pens were used and were continuously monitored using a digital camera system. For the automatic determination of the x and y coordinates of a cow, trajectory analysis was conducted using thermal
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Functional characterization of testis-brain RNA-binding protein, TB-RBP/Translin, in translational regulation J. Reprod. Dev. (IF 1.735) Pub Date : 2020-12-02 Kanako OYAMA; Tadashi BABA; Shin-ichi KASHIWABARA
Testis-brain RNA-binding protein (TB-RBP/Translin) is known to contribute to the translational repression of a subset of haploid cell-specific mRNAs, including protamine 2 (Prm2) mRNA. Mutant mice lacking TB-RBP display abnormal spermatogenesis, despite normal male fertility. In this study, we carried out functional analysis of TB-RBP in mammalian cultured cells to understand the mechanism of translational
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Different response of embryos originating from control and obese mice to insulin in vitro J. Reprod. Dev. (IF 1.735) Pub Date : 2020-11-27 Martina KŠIŇANOVÁ; Veronika KOVAŘÍKOVÁ; Zuzana ŠEFČÍKOVÁ; Alexandra ŠPIRKOVÁ; Štefan ČIKOŠ; Jozef PISKO; Dušan FABIAN
The aim of the present work was to investigate the impact of maternal obesity on DNA methylation in ovulated oocytes, and to compare the response of in vitro-developing preimplantation embryos originating from control and obese mice to insulin. An intergenerational, diet-induced obesity model was used to produce outbred mice with an increased body weight and body fat. Two-cell and eight-cell embryos
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Clinical prospects proposing an increase in the luteolytic dose of prostaglandin F2α in dairy cattle J. Reprod. Dev. (IF 1.735) Pub Date : 2020-11-09 Fernando LÓPEZ-GATIUS
Over the past few decades, the luteolytic dose of prostaglandin F2α (PGF2α) and its analogs, used to synchronize estrus for fixed-time insemination in dairy cattle, have remained unchanged. Given the beneficial effects of PGF2α on a young corpus luteum and on multiple ovulations in a fixed-time insemination protocol, and its therapeutic abortive effects on multiple ovulations in pregnant cows, we propose
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In vitro growth of bovine oocytes in oocyte-cumulus cell complexes and the effect of follicle stimulating hormone on the growth of oocytes J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-30 Mihoko FUSHII; Rie YAMADA; Takashi MIYANO
Several successful in vitro culture experiments have used oocyte-cumulus cell-mural granulosa cell complexes (OCGCs) from early antral follicles (0.5–0.7 mm) for the growth of bovine oocytes. However, in studies related to in vitro oocyte maturation and in vitro embryo production, oocyte-cumulus cell complexes (OCCs) that have no mural granulosa cells have been widely used instead of OCGCs. The purpose
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Expression and possible roles of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cell development. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Maria Sorrenti,Francesca Gioia Klinger,Sara Iona,Valerio Rossi,Serena Marcozzi,Massimo DE Felici
In the present work, we described the expression and activity of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cells (PGCs) from 8.5–14.5 days post coitum (dpc) and investigated whether these kinases play a role in regulating the various processes of PGC development. Using immunofluorescence and immunoblotting to detect the active phosphorylated form of ERK1-2 (p-ERK1-2)
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H4K20 monomethylation inhibition causes loss of genomic integrity in mouse preimplantation embryos. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Daiki Shikata,Takuto Yamamoto,Shinnosuke Honda,Shuntaro Ikeda,Naojiro Minami
Maintaining genomic integrity in mammalian early embryos, which are deficient in DNA damage repair, is critical for normal preimplantation and subsequent development. Abnormalities in DNA damage repair in preimplantation embryos can cause not only developmental arrest, but also diseases such as congenital disorders and cancers. Histone H4 lysine 20 monomethylation (H4K20me1) is involved in DNA damage
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Effect of neurotensin on cultured mouse preimplantation embryos. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Yuki Hiradate,Kenshiro Hara,Kentaro Tanemura
Previously, we revealed that neurotensin (NTS) derived from the oviduct and uterus can function during fertilization. However, little is known about NTS actions on the pre-implantation embryo after fertilization. Here, we found that pro-Nts mRNA is expressed in the oviduct and uterus during when preimplantation embryos develop and an increase in mRNA level in the uterus is induced by human chorionic
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Improvement of a twice collection method of mouse oocytes by surgical operation. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Rei Inoue,Kana Harada,Sayaka Wakayama,Masatoshi Ooga,Teruhiko Wakayama
Mouse oocytes are generally collected after euthanasia. However, if oocytes were collected without euthanasia, then mice could be used to collect oocytes again after recovery. This condition is especially useful for mice that are genotypically rare. In this study, we examined the reusability of mice after collecting oocytes via a surgical operation. When oocytes were collected using medetomidine/m
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Transcriptional activation of the mouse Scd2 gene by interdependent enhancers and long noncoding RNAs in ovarian granulosa cells. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Shota Mayama,Nobuhiko Hamazaki,Yuki Maruyama,Shin Matsubara,Atsushi P Kimura
Specific gene expression in granulosa cells is key for the function of ovary, but the molecular mechanism of transcriptional activation is not well studied. Here we investigated the regulatory mechanism of the mouse stearoyl-CoA desaturase 2 (Scd2) gene encoding an enzyme for lipid metabolism. Northern blot and in situ hybridization indicated that the mouse Scd2 mRNA was highly expressed in ovarian
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Relationship between sire predicted transmitting ability for daughter pregnancy rate and daughter's reproductive performance and milk production in Japanese dairy herds. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Keisuke Koyama,Takayoshi Takahashi
Modern genetic improvement in dairy cattle is directed towards improvement of fertility; however, reproduction traits generally exhibit a genetic antagonism with milk yield. Herein, we aimed to clarify the effects of sire predicted transmitting ability (PTA) for daughter pregnancy rate (DPR) on the reproductive performance and milk yield of daughters in Japanese dairy herds. We conducted a retrospective
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Artificial lactation by exogenous hormone treatment in non-pregnant sows. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Michiko Noguchi,Takehito Suzuki,Reiichiro Sato,Yosuke Sasaki,Kazuyuki Kaneko
This study aimed to determine if lactation can be induced by exogenous hormonal treatment in non-pregnant sows. In experiment 1, pseudopregnant animals were divided into four groups and given: 1) 5 mg of estradiol dipropionate (EDP) 5 days before (n = 4), 2) 5 mg of EDP 10 days before (n = 3), 3) 10 mg of EDP 5 days before (n = 3) or 4) 10 mg of EDP 10 days (n = 3) before PGF2α treatment. Artificial
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RNA-binding protein Ptbp1 regulates alternative splicing and transcriptome in spermatogonia and maintains spermatogenesis in concert with Nanos3. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Manami Senoo,Hiroshi Hozoji,Yu Ishikawa-Yamauchi,Takashi Takijiri,Sho Ohta,Tomoyo Ukai,Mio Kabata,Takuya Yamamoto,Yasuhiro Yamada,Masahito Ikawa,Manabu Ozawa
PTBP1, a well-conserved RNA-binding protein, regulates cellular development by tuning posttranscriptional mRNA modification such as alternative splicing (AS) or mRNA stabilization. We previously revealed that the loss of Ptbp1 in spermatogonia causes the dysregulation of spermatogenesis, but the molecular mechanisms by which PTBP1 regulates spermatogonium homeostasis are unclear. In this study, changes
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Production of genome-edited mice by visualization of nucleases introduced into the embryos using electroporation. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Yui Wake,Takehito Kaneko
Genome editing technology contributes to the quick and highly efficient production of genetically engineered animals. These animals are helpful in clarifying the mechanism of human disease. Recently, a new electroporation technique (TAKE: Technique for animal knockout system by electroporation) was developed to produce genome-edited animals by introducing nucleases into intact embryos using electroporation
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Iron deficiency induces female infertile in order to failure of follicular development in mice. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Shingo Tonai,Akane Kawabata,Tomoya Nakanishi,Joo Yeon Lee,Asako Okamoto,Masayuki Shimada,Yasuhisa Yamashita
Iron is important for many cellular functions, including ATP synthesis and cell proliferation. Insufficient of iron in the diet causes iron deficiency anemia (IDA), which often occurs in people living in the world. Since 50% of women with IDA show amenorrhea, the relationship of between iron deficiency and reproductive function was assessed using mice fed a low Fe diet (LFD). The estrous cycle in the
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Effects of twin pregnancy prevention strategies such as GnRH dose and drainage of the smaller follicle on ovulation in dairy cows with two follicles of pre-ovulatory size in the same ovary. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Irina Garcia-Ispierto,Fernando López-Gatius
We examined the effects of a single or 2.5-fold dose of dephereline [a gonadotropin-releasing hormone (GnRH) analogue] as well as the drainage of the smaller follicle at the time of insemination on ovulation in dairy cows with two follicles of pre-ovulatory size in the same ovary. The three study groups included 220 monovular cows (control), 110 non-drained cows with two follicles, and 110 cows with
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Intravaginal administration of progesterone using a new technique for sustained drug release in goats. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-13 Natsumi Endo,Larasati Puji Rahayu,Takashi Yamamura,Hitoshi Tanaka,Tomomi Tanaka
The objective of the present study was to develop and evaluate a sustained release vaginal progesterone (P4) capsule containing a mixture of mucoadhesive polymer and silicone fluid. Goats were administered a gelatin capsule containing 0.4 g of P4 mixed in silicone fluid and either a hydroxypropylmethylcellulose (HM) or polyaclil starch (PA) base. The mean plasma P4 concentrations at 2 and 12 h after
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Estrogen increases KISS1 expression in newly generated immortalized KISS1-expressing cell line derived from goat preoptic area J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-25 Yukina OSHIMO; Arisa MUNETOMO; Fumie MAGATA; Yuta SUETOMI; Shuhei SONODA; Yukari TAKEUCHI; Hiroko TSUKAMURA; Satoshi OHKURA; Fuko MATSUDA
Kisspeptin neurons located in the hypothalamic preoptic area (POA) are suggested to be responsible for the induction of the gonadotropin-releasing hormone (GnRH) surge and the following luteinizing hormone (LH) surge to regulate female mammals’ ovulation. Accumulating evidence demonstrates that the preovulatory level of estrogen activates the POA kisspeptin neurons (estrogen positive feedback), which
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Impact of short-term high-fat feeding on lipid droplet content in mouse oocytes J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-17 Ryutaro AIZAWA; Megumi IBAYASHI; Tomomi HATAKEYAMA; Takayuki TATSUMI; Satoshi TSUKAMOTO
Mature mammalian oocytes contain lipid droplets (LDs), which are neutral lipid storage organelles critically important for energy metabolism. In mice, maternal obesity, induced by long-term (> 3 months) high-fat feeding, contributes to the accumulation of LDs in mature oocytes. However, few studies have investigated the influence of short-term high-fat feeding on LD content. In this study, we demonstrated
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An attempt at estrus detection in cattle by continuous measurements of ventral tail base surface temperature with supervised machine learning J. Reprod. Dev. (IF 1.735) Pub Date : 2020-10-09 Shogo HIGAKI; Hongyu DARHAN; Chie SUZUKI; Tomoko SUDA; Reina SAKURAI; Koji YOSHIOKA
We aimed to determine the effectiveness of estrus detection based on continuous measurements of the ventral tail base surface temperature (ST) with supervised machine learning in cattle. ST data were obtained through 51 estrus cycles on 11 female cattle (six Holsteins and five Japanese Blacks) using the tail-attached sensor. Three estrus detection models were constructed with the training data (n =
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Transfer of a single embryo versus drainage of subordinate follicles to prevent twin pregnancies in dairy cows. Why not both? J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Fernando LÓpez-Gatius,Irina Garcia-Ispierto
In this study, we present two proposed approaches to prevent twin pregnancies in dairy cattle: 1) single, in vitro-produced embryonic transfer into a recipient cow or 2) subordinate follicle drainage at the time of insemination. Both procedures lead to improved embryonic survival. As the use of sexed semen generates herd replacements and additional heifers, we propose the transfer of a single female
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Discovery of new receptors regulating luteinizing hormone and follicle-stimulating hormone secretion by bovine gonadotrophs to explore a new paradigm for mechanisms regulating reproduction. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Hiroya Kadokawa
Previous studies in the 1960s and 1970s have reported that both gonadotropin-releasing hormone (GnRH) and estradiol-activated nuclear estrogen receptors regulate gonadotropin secretion in women. However, I had previously reported that gonadotroph function is regulated by complex crosstalk between several membrane receptors. RNA-seq had previously revealed 259 different receptor genes expressed in the
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Development of assisted reproductive technologies in small animal species for their efficient preservation and production. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Keiji Mochida
Assisted reproductive technologies (ARTs) are widely used in the animal industry, human clinics, and for basic research. In small laboratory animal species such as mice, ARTs are essential for the production of animals for experiments, the preservation of genetic resources, and for the generation of new strains of genetically modified animals. The RIKEN BioResource Research Center (BRC) is one of the
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Multiple roles of hypoxia in bovine corpus luteum. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Ryo Nishimura,Kiyoshi Okuda
There has been increasing interest in the role of hypoxia in the microenvironment of organs, because of the discovery of hypoxia-inducible factor-1 (HIF1), which acts as a transcription factor for many genes activated specifically under hypoxic conditions. The ovary changes day by day during the estrous cycle as it goes through phases of follicular growth, ovulation, and formation and regression of
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Lipopolysaccharide-induced mechanisms of ovarian dysfunction in cows with uterine inflammatory diseases. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Fumie Magata
Uterine inflammatory diseases commonly occur in postpartum dairy cows, resulting in reduced reproductive performance due to aberrant uterine and ovarian activity. Infection of the uterus with gram-negative bacteria results in the detection of lipopolysaccharide (LPS) in the plasma and follicular fluid of cows along with uterine inflammation. LPS acts on follicular components such as theca cells, granulosa
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Canine decidualization in vitro: extracellular matrix modification, progesterone mediated effects and selective blocking of prostaglandin E2 receptors. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Felix R Graubner,Miguel Tavares Pereira,Alois Boos,Mariusz P Kowalewski
Recently, we established an in vitro model with immortalized dog uterine stromal (DUS) cells for investigations into canine-specific decidualization. Their capability to decidualize was assessed with cAMP and prostaglandin (PG) E2. Here, we show that the effects of PGE2 are mediated through both of the cAMP-mediating PGE2 receptors (PTGER2/4). Their functional inhibition suppressed gene expression
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Transcriptome analysis to unravel the gene expression profile of ovarian follicular development in Magang goose. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Mingming Lei,Rong Chen,Qingming Qin,Huanxi Zhu,Zhendan Shi
Magang geese exhibit a unique characteristic of follicular development, with eight largest orderly arranged pre-ovulatory follicles in the abdominal cavity. However, little is known about the mechanisms underlying this follicular development. This study aimed to compare gene expression profiles of granulosa cells (GCs) at different stages of follicular development and provide comprehensive insights
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CD2 is a surface marker for mouse and rat spermatogonial stem cells. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Mito Kanatsu-Shinohara,Guiying Chen,Hiroko Morimoto,Takashi Shinohara
The spermatogonial stem cell (SSC) population in testis is small, and the lack of SSC markers has severely handicapped research on these cells. During our attempt to identify genes involved in SSC aging, we found that CD2 is expressed in cultured SSCs. Flow cytometric analysis and spermatogonial transplantation experiments showed that CD2 is expressed in SSCs from mature adult mouse testes. Cultured
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Peripheral administration of SB223412, a selective neurokinin-3 receptor antagonist, suppresses pulsatile luteinizing hormone secretion by acting on the gonadotropin-releasing hormone pulse generator in estrogen-treated ovariectomized female goats. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Takuya Sasaki,Tomoya Sonoda,Ryoki Tatebayashi,Yuri Kitagawa,Shinya Oishi,Koki Yamamoto,Nobutaka Fujii,Naoko Inoue,Yoshihisa Uenoyama,Hiroko Tsukamura,Kei-Ichiro Maeda,Fuko Matsuda,Yasuhiro Morita,Shuichi Matsuyama,Satoshi Ohkura
Accumulating evidence suggests that KNDy neurons located in the hypothalamic arcuate nucleus (ARC), which are reported to express kisspeptin, neurokinin B, and dynorphin A, are indispensable for the gonadotropin-releasing hormone (GnRH) pulse generation that results in rhythmic GnRH secretion. The aims of the present study were to investigate the effects of peripheral administration of the neurokinin
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Conditional kisspeptin neuron-specific Kiss1 knockout with newly generated Kiss1-floxed and Kiss1-Cre mice replicates a hypogonadal phenotype of global Kiss1 knockout mice. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Kana Ikegami,Teppei Goto,Sho Nakamura,Youki Watanabe,Arisa Sugimoto,Sutisa Majarune,Kei Horihata,Mayuko Nagae,Junko Tomikawa,Takuya Imamura,Makoto Sanbo,Masumi Hirabayashi,Naoko Inoue,Kei-Ichiro Maeda,Hiroko Tsukamura,Yoshihisa Uenoyama
The present study aimed to evaluate whether novel conditional kisspeptin neuron-specific Kiss1 knockout (KO) mice utilizing the Cre-loxP system could recapitulate the infertility of global Kiss1 KO models, thereby providing further evidence for the fundamental role of hypothalamic kisspeptin neurons in regulating mammalian reproduction. We generated Kiss1-floxed mice and hypothalamic kisspeptin neuron-specific
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Inducible Kiss1 knockdown in the hypothalamic arcuate nucleus suppressed pulsatile secretion of luteinizing hormone in male mice. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Shiori Minabe,Sho Nakamura,Eri Fukushima,Marimo Sato,Kana Ikegami,Teppei Goto,Makoto Sanbo,Masumi Hirabayashi,Junko Tomikawa,Takuya Imamura,Naoko Inoue,Yoshihisa Uenoyama,Hiroko Tsukamura,Kei-Ichiro Maeda,Fuko Matsuda
Accumulating evidence suggests that kisspeptin-GPR54 signaling is indispensable for gonadotropin-releasing hormone (GnRH)/gonadotropin secretion and consequent reproductive functions in mammals. Conventional Kiss1 knockout (KO) mice and rats are reported to be infertile. To date, however, no study has investigated the effect of inducible central Kiss1 KO/knockdown on pulsatile gonadotropin release
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Ruthenium red improves blastocyst developmental competence by regulating mitochondrial Ca2+ and mitochondrial functions in fertilized porcine oocytes in vitro. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Ho-Geun Jegal,Hyo-Jin Park,Jin-Woo Kim,Seul-Gi Yang,Min-Ji Kim,Deog-Bon Koo
Ruthenium red (RR) inhibits calcium (Ca2+) entry from the cytoplasm to the mitochondria, and is involved in maintenance of Ca2+ homeostasis in mammalian cells. Ca2+ homeostasis is very important for further embryonic development of fertilized oocytes. However, the effect of RR on mitochondria-Ca2+ (mito-Ca2+) levels during in vitro fertilization (IVF) on subsequent blastocyst developmental capacity
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Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-08-20 Sevastiani Antonouli,Maria Grazia Palmerini,Serena Bianchi,Gianna Rossi,Sandra Cecconi,Manuel Belli,Sara Bernardi,Mohammad Ali Khalili,Giuseppe Familiari,Stefania Annarita Nottola,Guido Macchiarelli
Controlled ovarian hyperstimulation (COH) is routinary used in assisted reproductive technologies (ARTs) to increase the yields of mature oocytes. The possibility that patients with a history of failures or poor-responders may develop side-effects following these treatments is still debated. Epidemiological studies reported controversial results about pregnancy outcome and the risk of developing gynecological
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Influence of ipsilateral coexistence of the first wave dominant follicle and corpus luteum on ovarian dynamics and plasma sex steroid hormone concentrations in lactating dairy cows treated with human chorionic gonadotropin. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-04-07 Ryotaro Miura,Nobu Matsumoto,Shingo Haneda,Motozumi Matsui
We examined the effect of human chorionic gonadotropin (hCG) treatment 5 days after estrus on ovarian dynamics and plasma progesterone (P4) and estradiol (E2) concentrations when the first-wave dominant follicle (DF) was ipsilateral or contralateral to the corpus luteum (CL) in lactating dairy cows. Seventy cows were divided into two groups: (1) ipsilateral group (IG; n = 37), in which the first-wave
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Developmental competence of interspecies cloned embryos produced using cells from large Japanese field mice (Apodemus speciosus) and oocytes from laboratory mice (Mus musculus domesticus). J. Reprod. Dev. (IF 1.735) Pub Date : 2020-03-26 Rika Azuma,Yuki Hatanaka,Seung-Wook Shin,Hitoshi Murai,Minoru Miyashita,Masayuki Anzai,Kazuya Matsumoto
The large Japanese field mouse (Apodemus speciosus) is endemic to Japan and may be used as an animal model for studies related to environmental pollution, medical science, and basic biology. However, the large Japanese field mouse has low reproductive ability due to the small number of oocytes ovulated per female. To produce experimental models, we investigated the in vitro developmental potential
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Necessity of exact examination of sperm characteristics to assess artificial insemination-subfertile bulls. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-03-19 Mitsuhiro Sakase,Moriyuki Fukushima
Conception rates of artificial insemination (AI) have gradually been decreasing in the cattle. In order to overcome this problem, AI centers need supply high-quality frozen semen whose insemination makes cow pregnant efficiently. Semen quality is conventionally assessed under the light microscope with cell biological methods, and only high-quality frozen semen straws are used for AI. However, lower
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An investigation of the time period within which frozen-thawed semen delivers a high conception rate in lactating dairy cows. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-03-16 Toshiaki Sumiyoshi,Tomomi Tanaka,Hideo Kamomae
We determined the length of time within which frozen-thawed semen delivered a high conception rate in present-day lactating dairy cows. The cows utilized were a total 100 milking Holstein-Friesian cows kept in tie-stall style farms. We carried out artificial insemination (AI) during the periovulatory period at a predicted time based on ovulation, and checked ovulation at 6-h intervals after AI. The
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Combined refinements to somatic cell nuclear transfer methods improve porcine embryo development. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-03-14 Thanh Quang Dang-Nguyen,David Wells,Seiki Haraguchi,Nguyen Thi Men,Hiep Thi Nguyen,Junko Noguchi,Hiroyuki Kaneko,Kazuhiro Kikuchi
The discovery of how to utilize CRISPR (clustered, regularly interspaced, short, palindromic repeats)-Cas (CRISPR-associated) systems for genome modification has accelerated development of the field of genome editing, especially in large animals such as pigs. The low efficiency of somatic cell nuclear transfer (SCNT) is now becoming a major obstacle in the production of genome-edited animals via cell-mediated
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Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-02-28 Chikaya Deura,Yusuke Kimura,Takumi Nonoyama,Ryutaro Moriyama
GPR120 is a long-chain fatty acid (LCFA) receptor that is specifically expressed in gonadotropes in the anterior pituitary gland in mice. The aim of this study was to investigate whether GPR120 is activated by free fatty acids in the pituitary of mice and mouse immortalized gonadotrope LβT2 cells. First, the effects of palmitate on GPR120, gonadotropic hormone b-subunits, and GnRH-receptor expression
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Palmitic acid activates NLRP3 inflammasome and induces placental inflammation during pregnancy in mice. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-02-27 Michiya Sano,Sayaka Shimazaki,Yasuaki Kaneko,Tadayoshi Karasawa,Masafumi Takahashi,Akihide Ohkuchi,Hironori Takahashi,Akira Kurosawa,Yasushi Torii,Hisataka Iwata,Takehito Kuwayama,Koumei Shirasuna
Maternal obesity is one of the major risk factors for pregnancy complications and is associated with low-grade chronic systemic inflammation due to higher levels of pro-inflammatory cytokines such as interleukin (IL)-1β. Pregnant women with obesity have abnormal lipid profiles, characterized by higher levels of free fatty acids, especially palmitic acid (PA). Previously, we reported that PA stimulated
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Establishment of long-term chronic recording technique of in vivo ovarian parenchymal temperature in Japanese Black cows. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-02-17 Yasuhiro Morita,Riho Ozaki,Akihisa Mukaiyama,Takuya Sasaki,Ryoki Tatebayashi,Ai Morishima,Yuri Kitagawa,Reika Suzumura,Ryoya Abe,Hiroko Tsukamura,Shuichi Matsuyama,Satoshi Ohkura
The reproductive performance of cattle can be suppressed by heat stress. Reproductive organ temperature, especially ovarian temperature, may affect follicle development and ovulation. The establishment of a technique for long-term measurement of ovarian temperature could prove useful in understanding the mechanisms underlying the temperature-dependent changes in follicular development and subsequent
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miR-431 regulates granulosa cell function through the IRS2/PI3K/AKT signaling pathway. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-02-13 Lei Yang,Qizhuang Lv,Jianyun Liu,Shikai Qi,Denggang Fu
MicroRNAs (miRNAs) regulate the functions of granulosa cells by interacting with their target mRNAs. Insulin receptor substrate 2 (IRS2) is one of the targets of miR-431 and can be regulated by ovarian hormones. However, the role of miR-431 and the associated signal transduction pathway in ovarian development has not been studied previously. In this study, we first analyzed the expression of miR-431
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Season does not have a deleterious effect on proportions of stallion seminal plasma proteins. J. Reprod. Dev. (IF 1.735) Pub Date : 2020-02-13 Anders Johannisson,Essraa M Al-Essawe,Anas Kh Al-Saffar,Saeid Karkehabadi,Isabel Lima-Verde,Manuela Wulf,Christine Aurich,Jane M Morrell
The mechanism by which the content of the major groups of seminal plasma proteins in stallion semen changes between the breeding and non-breeding seasons remains unknown. Here, we investigated the proportions of non-heparin-binding, phosphorylcholine-binding, and heparin-binding proteins in seminal plasma with the aim of relating them to sperm quality and testosterone levels in good and bad freezer
更新日期:2020-02-13