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Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves Toxicol. In Vitro (IF 3.2) Pub Date : 2024-03-12 Catherine Martin, Bertrand Evrard, Frederic Percevault, Kate Ryder, Thomas Darde, Aurélie Lardenois, Maxim Zhadobov, Ronan Sauleau, Frédéric Chalmel, Yves Le Dréan, Denis Habauzit
The use of millimeter waves (MMW) will exponentially grow in the coming years due to their future utilization in 5G/6G networks. The question of possible biological effects at these frequencies has been raised. In this present study, we aimed to investigate gene expression changes under exposure to MMW using the Bulk RNA Barcoding and sequencing (BRB-seq) technology. To address this issue, three exposure
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Metformin and buparlisib synergistically induce apoptosis of non-small lung cancer (NSCLC) cells via Akt/FoxO3a/Puma axis Toxicol. In Vitro (IF 3.2) Pub Date : 2024-03-12 Wang Shanshan, Ma Hongying, Fang Jingjing, Yu Rui
Non-small cell lung cancer (NSCLC) is a global health issue lacking effective treatments. Buparlisib is a pan-PI3K inhibitor that shows promising clinical results in treating NSCLC. However, chemoresistance is inevitable and hampers the application of buparlisib. Studies show that a combination of phytochemicals and chemotherapeutics enhances its effectiveness. Here, we evaluated the role of metformin
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In vitro assessment of cytotoxicity of spent fluid catalytic cracking refinery catalysts on cell lines and identification of critical toxic metals Toxicol. In Vitro (IF 3.2) Pub Date : 2024-03-06 Yue-jie Wang, Zhi-guo Sheng, Jun Li, Zhen-feng Zhao, Ling-ling Li, Chen Shen
The Purpose of the present study was to quantify the responses of ten cell lines (HeLa, HepG2, HEK293, MDA-MB-231, A498, A549, A357, 3 T3, BALB-C3 T3, and NIH-3 T3) to spent fluid catalytic cracking catalysts (SFCCCs) from different petroleum refineries, and relate these responses to metal concentrations of SFCCC leachates (SFCCCLs). Cytotoxicity of SFCCCs were significantly different depending on
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Simulated gastric leachate of 3D printer metal-fill filaments induces cytotoxic effects in rat and human intestinal models Toxicol. In Vitro (IF 3.2) Pub Date : 2024-03-06 Michael F. Hughes, Haley M. Clapper, Getachew Tedla, Tyler D. Sowers, Kim R. Rogers
Metals are used in 3-dimensional (3D) printer filaments in the manufacture of 3D printed objects. Exposure to the filaments, printed objects and emissions from printing may pose health risks from release of toxic metals. This study investigated the cytotoxicity of extruded 3D printer filament leachates in rat and human intestinal cells. Copper-, bronze-, and steel-fill extruded filaments were incubated
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Transcriptomic profile of human iPSC-derived podocyte-like cells exposed to a panel of xenobiotics Toxicol. In Vitro (IF 3.2) Pub Date : 2024-03-04 Cormac Murphy, Paul Jennings, Anja Wilmes
Podocytes play a critical role in the formation and maintenance of the glomerular filtration barrier and injury to these cells can lead to a breakdown of the glomerular barrier causing permanent damage leading to progressive chronic kidney disease. Matured podocytes have little proliferative potential, which makes them critical cells from a health perspective, but also challenging cells to maintain
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Lovastatin impairs cellular proliferation and enhances hyaluronic acid production in fibroblast-like synoviocytes Toxicol. In Vitro (IF 3.2) Pub Date : 2024-03-01 Wen-Tien Wu, Chen-Chie Wang, Dai-Hua Lu, Kuan-Jung Lu, Yu-Chia Chang, Kai-Chiang Yang
Statins have demonstrated chondroprotective effects by reducing inflammation and mitigating extracellular matrix degradation. However, statins are also reported to be cytotoxic to several types of cells. Early-onset osteoarthritis (OA) is characterized by synovial inflammation, which adversely affects hyaluronan (HA) production in fibroblast-like synoviocytes (FLSs). Nevertheless, the precise effects
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Using cell culture systems from the Persian Gulf Arabian yellowfin sea bream, Acanthopagrus arabicus, to assess the effects of dexamethasone on gonad and brain aromatase activity and steroid production Toxicol. In Vitro (IF 3.2) Pub Date : 2024-03-01 Zahra Balasem, Negin Salamat, Hoda Mojiri-Forushani
Dexamethasone (DEX) is a synthetic glucocorticoid widely used as pharmaceutical and usually exists in effluents with varying degrees of concentrations. In this study, cultivated Brain, ovary and testis cells from Arabian Sea bream, were treated by DEX at concentrations of 0, 0.3, 3.0, 30.0 and 300.0 μg/ml for 48 h. The aromatase activity and steroid (17-β-estradiol (E2), progesterone (P) and testosterone
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Characterization of palmitic acid toxicity induced insulin resistance in HepG2 cells. Toxicol. In Vitro (IF 3.2) Pub Date : 2024-02-29 Sajad Malik, Shrirang Inamdar, Jhankar Acharya, Pranay Goel, Saroj Ghaskadbi
An etiology of palmitic acid (PA) induced insulin resistance (IR) is complex for which two mechanisms are proposed namely ROS induced JNK activation and lipid induced protein kinase-C (PKCε) activation. However, whether these mechanisms act alone or in consortium is not clear. In this study, we have characterized PA induced IR in liver cells. These cells were treated with different concentrations of
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Hsa_circ_0003356 suppresses gastric cancer progression via miR-556-5p/FKBP5 axis Toxicol. In Vitro (IF 3.2) Pub Date : 2024-02-22 Chuanhui Lu, Jing Wu, Xiaoguang Li, Wei Huang, Yongmu Fang, Ying Huang
CircRNAs are implicated in the tumorigenesis of various human cancers. This study aims to explore how circ_0003356 contributes to the development of gastric cancer (GC). Circ_0003356 expression was analyzed in GSE184882 dataset and validated in our cohort of GC patients and human GC cell lines. The correlations between circ_0003356 levels and prognostic parameters were analyzed. The contribution of
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In vitro test methods for evaluating high molecular weight polyethylene oxide polymer induced hemolytic and thrombotic potential Toxicol. In Vitro (IF 3.2) Pub Date : 2024-02-22 Dongjune Kim, Rucha Natu, Richard Malinauskas, Jin Hyen Baek, Paul W Buehler, Xin Feng, Haiou Qu, Julia Pinto, Xiaoming Xu, Luke Herbertson
To combat opioid abuse, the U.S. Food and Drug Administration (FDA) released a comprehensive action plan to address opioid addiction, abuse, and overdose that included increasing the prevalence of abuse-deterrent formulations (ADFs) in opioid tablets. Polyethylene oxide (PEO) has been widely used as an excipient to deter abuse nasal insufflation. However, changes in abuse patterns have led to unexpected
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A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards Toxicol. In Vitro (IF 3.2) Pub Date : 2024-02-15 Polly McLean, Jessica Marshall, Amaia García, David Beal, Alberto Katsumiti, Marie Carrière, Matthew Boyles
The objective of Safe-by-Design (SbD) is to support the development of safer products and production processes, and enable safe use throughout a materials' life cycle; an intervention at an early stage of innovation can greatly benefit industry by reducing costs associated with the development of products later found to elicit harmful effects. Early hazard screening can support this process, and is
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Gill epithelial cell line ASG-10 from Atlantic salmon as a new research tool for solving water quality challenges in aquaculture Toxicol. In Vitro (IF 3.2) Pub Date : 2024-02-13 Anita Solhaug, Pål A. Olsvik, Prabhugouda Siriyappagouder, Randi Faller, Torstein Kristensen
Here we evaluated the gill epithelial cell line ASG-10 from Atlantic salmon, as an model for research on known water quality challenges in aquaculture. Ammonia/ammonium (NH/NH), a recognized challenge in water-intensive recirculating aquaculture systems (RAS), induced lysosomal vacuolization, reduced protein degradation and cell migration of the ASG-10 cells. Aluminium (Al), another challenge in freshwater
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Development of natural cosmetic emulsion using the by-product of Lecythis pisonis seed Toxicol. In Vitro (IF 3.2) Pub Date : 2024-02-13 Danielle Braga Portes, Lucas Wetler Abeldt, Cristiane dos Santos Giuberti, Denise Coutinho Endringer, Elisângela Flávia Pimentel
Natural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of was applied to develop a natural cosmetic emulsion. To ensure safety for its topical
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Interaction of mycotoxins zearalenone, α-zearalenol, and β-zearalenol with cytochrome P450 (CYP1A2, 2C9, 2C19, 2D6, and 3A4) enzymes and organic anion transporting polypeptides (OATP1A2, OATP1B1, OATP1B3, and OATP2B1) Toxicol. In Vitro (IF 3.2) Pub Date : 2024-02-08 Hana Kaci, Ágnes Dombi, Patrik Gömbös, András Szabó, Éva Bakos, Csilla Özvegy-Laczka, Miklós Poór
Zearalenone (ZEN) is a mycoestrogen produced by fungi. ZEN is a frequent contaminant in cereal-based products, representing significant health threat. The major reduced metabolites of ZEN are α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL). Since the toxicokinetic interactions of ZEN/ZELs with cytochrome P450 enzymes (CYPs) and organic anion transporting polypeptides (OATPs) have been barely characterized
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Purinergic ligands induce extracellular acidification and increased ATP turnover in HepG2 cells Toxicol. In Vitro (IF 3.2) Pub Date : 2024-02-04 Haotong Chen, Yong Han, Abby Hearne, Anna Monarchino, Jeffrey S. Wiseman
Nucleosides and nucleotides at μM concentrations stimulated a 300% increase in acid secretion in HepG2 cells, which was quantitatively accounted for as increased export of lactate generated by glycogenolysis. Agonist selectivity encompassed nucleosides and nucleotides for all 5 natural nucleobases and, along with antagonist profiles, was inconsistent with a role for purinergic receptors in mediating
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Anti-migratory and cytotoxic effect of indole derivative in C6 glioma cells Toxicol. In Vitro (IF 3.2) Pub Date : 2024-02-01 Ana Karolina de Souza Andrade, Mariana Nobre Farias de Franca, Jileno Ferreira Santos, Nathália Araújo Macêdo, Waldecy de Lucca Junior, Ricardo Scher, Socrates Cabral de Holanda Cavalcanti, Cristiane Bani Corrêa
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Arsenic trioxide inhibits the response of primary human B cells to influenza virus A in vitro Toxicol. In Vitro (IF 3.2) Pub Date : 2024-01-24 Luca M. Kaiser, Robert A. Freeborn, Allison P. Boss, Yining Jin, Cheryl E. Rockwell
Arsenic compounds are common environmental toxicants worldwide and particularly enriched in the Northeast and the Southwestern United States, the Alps, and Bangladesh. Exposure to arsenic is linked with various detrimental health outcomes, including cancer, cognitive decline, and kidney damage. Our group has previously shown that arsenic trioxides alter T cell cytokine production. In the current study
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Biochanin A – A G6PD inhibitor: In silico and in vitro studies in non-small cell lung cancer cells (A549) Toxicol. In Vitro (IF 3.2) Pub Date : 2024-01-23 Anjali B. Thakkar, Ramalingam B. Subramanian, Sampark S. Thakkar, Vasudev R. Thakkar, Parth Thakor
Secondary metabolites from medicinal plants have a well-established therapeutic potential, with many of these chemicals having specialized medical uses. Isoflavonoids, a type of secondary metabolite, have little cytotoxicity against healthy human cells, making them interesting candidates for cancer treatment. Extensive research has been conducted to investigate the chemo-preventive benefits of flavonoids
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Ursodeoxycholic acid and 18β-glycyrrhetinic acid alleviate ethinylestradiol-induced cholestasis via downregulating RORγt and CXCR3 signaling pathway in iNKT cells Toxicol. In Vitro (IF 3.2) Pub Date : 2024-01-18 Xinyu Li, Xiaojing Liang, Xiaoxia Gu, Mengzhi Zou, Weiping Cao, Chunhui Liu, Xinzhi Wang
Estrogen-induced intrahepatic cholestasis (IHC) is a mild but potentially serious risk and urges for new therapeutic targets and effective treatment. Our previous study demonstrated that RORγt and CXCR3 signaling pathway of invariant natural killer T (iNKT) 17 cells play pathogenic roles in 17α-ethinylestradiol (EE)-induced IHC. Ursodeoxycholic acid (UDCA) and 18β-glycyrrhetinic acid (GA) present a
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ESTIV 2022: Key enabling non-animal technologies for research, education and testing Toxicol. In Vitro (IF 3.2) Pub Date : 2024-01-12 Helena Kandarova, Clive Roper, Marketa Dvořáková
Abstract not available
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PBA alleviates cadmium-induced mouse spermatogonia apoptosis by suppressing endoplasmic reticulum stress Toxicol. In Vitro (IF 3.2) Pub Date : 2024-01-17 Rong Wang, Mengyuan Li, Zhen Wu, Wenjing Gong, Mingming Zhang, Yehao Liu, Yuyou Yao, Yanli Ji
Objective Endoplasmic reticulum (ER) stress mediates Cd-caused germ cell apoptosis in testis. The effects of 4-phenylbutyric acid (PBA), a classical chaperone, were investigated on Cd-induced apoptosis in mouse GC-1 spermatogonia cells. Methods The cells were pretreated with PBA before Cd exposure. TUNEL and flow cytometry assays were applied to determine apoptosis. Some key biomarkers of ER stress
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Particulate iron oxide food colorants (E 172) during artificial digestion and their uptake and impact on intestinal cells Toxicol. In Vitro (IF 3.2) Pub Date : 2024-01-08 Holger Sieg, Caroline Schaar, Nicole Fouquet, Linda Böhmert, Andreas F. Thünemann, Albert Braeuning
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Protective role of fetal bovine serum on PLHC-1 spheroids exposed to a mixture of plastic additives: A lipidomic perspective Toxicol. In Vitro (IF 3.2) Pub Date : 2024-01-03 Tiantian Wang, Judith Desmet, Cinta Porte
The use of fetal bovine serum (FBS) in cell culture is being questioned for scientific and ethical reasons, prompting the exploration of alternative approaches. Nevertheless, the influence of FBS on cell functioning, especially in fish cells, has not been comprehensively examined. This study aims to evaluate the impact of FBS on the lipidome of PLHC-1 spheroids and investigate cellular and molecular
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Screening for endocrine disrupting chemicals inhibiting monocarboxylate 8 (MCT8) transporter facilitated thyroid hormone transport using a modified nonradioactive assay Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-25 Fabian Wagenaars, Peter Cenijn, Martin Scholze, Caroline Frädrich, Kostja Renko, Josef Köhrle, Timo Hamers
Early neurodevelopmental processes are strictly dependent on spatial and temporally modulated of thyroid hormone (TH) availability and action. Thyroid hormone transmembrane transporters (THTMT) are critical for regulating the local concentrations of TH, namely thyroxine (T4) and 3,5,3′-tri-iodothyronine (T3), in the brain. Monocarboxylate transporter 8 (MCT8) is one of the most prominent THTMT. Genetically
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Fragrances as a trigger of immune responses in different environments Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-22 M. Macchione, K. Yoshizaki, D.P. Frias, K. Maier, J. Smelan, C.M. Prado, T. Mauad
Fragrances can cause allergic skin reactions, expressed as allergic contact dermatitis and reactions in the respiratory tract that range from acute temporary upper airway irritation to obstructive lung disease. These adverse health effects may result from the stimulation of a specific (adaptive) immune response. Th1 cells, which essentially produce interleukin-2 (IL-2) and interferon-γ (IFN-γ), play
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Comparison of in vitro thyroxine (T4) metabolism between Wistar rat and human hepatocyte cultures Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-22 Audrey Baze, Lucille Wiss, Liliia Horbal, Klaus Biemel, Laure Asselin, Lysiane Richert
In vitro assays remain relatively new in exploring human relevance of liver, in particular nuclear receptor-mediated perturbations of the hypothalamus-pituitary-thyroid axis seen in rodents, mainly in the rat. Consistent with in vivo data, we confirm that thyroid hormone thyroxine metabolism was 9 times higher in primary rat hepatocytes (PRH) than in primary human hepatocytes (PHH) cultured in a 2D
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Dinaciclib inhibits the growth of acute myeloid leukemia cells through either cell cycle-related or ERK1/STAT3/MYC pathways Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-21 Chieh-Lin Jerry Teng, Pang-Ting Cheng, Yu-Chiao Cheng, Jia-Rung Tsai, Mei-Chih Chen, Ho Lin
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Delayed clearance of the pro-carcinogen benzo[a]pyrene in PLHC-1 cells when co-exposed to the antifungal drug clotrimazole and effects on the CYP1A biomarker Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-19 Maja Edenius, Anne Farbrot, Anders Blom, Malin C. Celander
Cytochrome P450 1 A (CYP1A) is a key enzyme in the metabolism of the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BaP) in animals, and a biomarker for environmental PAH exposure. The common antimycotic imidazole drug clotrimazole (CLO) has been detected in the aquatic environment and likely co-exists with BaP. Like BaP, CLO can bind to CYP1A enzymes and can act as a CYP1A inhibitor. Co-exposure
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Organochlorine pesticides disrupt T helper cell regulation and reduce IL-2 and IFN-γ favoring infection and production of autoantibodies among pemphigus patients Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-13 Sajad Ahmad Dar, Shukla Das, Mohammed Ageeli Hakami, Naseem Akhter, Mohammad Mustafa, Arshad Jawed, Sambit Nath Bhattacharya, Basu Dev Banerjee, Abrar Ahmad
The list of environmental factors that trigger autoimmune diseases in genetically susceptible individuals has grown in the recent years and is far from complete. The possible intervention of the environment in triggering these diseases is ever more perceived by the clinicians. This study investigated the effect of environmental factors like organochlorine pesticides (OCPs) on proportions of different
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Reduced toxic effects of nano‑copper sulfate in comparison of bulk CuSO4 on biochemical parameters in the Rohu (Labeo rohita) Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-15 Azka Anwar, Fahim Ullah Khan, Waqar Younas, Muhib Zaman, Muhammad Noorullah, Li'ang Li, Amina Zuberi, Youji Wang
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Synthesis of homogeneous spherical selenium nanoparticles through a chemical method for cancer therapy applications Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-15 E.I. Adam-Dima, M. Balas, M. Anastasescu, C. Purdel, D. Margină
Oxidative stress is associated with pathologies affecting various organs or metabolic pathways. Thus, targeting oxidative stress might represent a valid therapeutic option. Selenium nanoparticles (SeNPs) are reported to exert antioxidant effects by many mechanisms. Our purpose was to assess in vitro on normal (MRC-5) and cancer (PANC-1) cell lines the potential of SeNPs for inducing cytotoxicity and
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In vitro screening of per- and polyfluorinated substances (PFAS) for interference with seven thyroid hormone system targets across nine assays Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-10 Sigmund J. Degitz, Jennifer H. Olker, Jeffery S. Denny, Philip P. Degoey, Phillip C. Hartig, Mary C. Cardon, Stephanie A. Eytcheson, Jonathan T. Haselman, Sally A. Mayasich, Michael W. Hornung
The US Environmental Protection Agency is evaluating the ecological and toxicological effects of per- and polyfluorinated chemicals. A number of perfluorinated chemicals have been shown to impact the thyroid axis in vivo suggesting that the thyroid hormone system is a target of these chemicals. The objective of this study was to evaluate the activity of 136 perfluorinated chemicals at seven key molecular
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Investigating the dependency of in vitro benchmark concentrations on exposure time in transcriptomics experiments Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-09 Donatella Carpi, Roman Liska, Julia M. Malinowska, Taina Palosaari, Mounir Bouhifd, Maurice Whelan
There is increasing interest to employ in vitro transcriptomics experiments in toxicological testing, for example to determine a point-of-departure (PoD) for chemical safety assessment. However current practices to derive PoD tend to utilise a single exposure time despite the importance of exposure time on the manifestation of toxicity caused by a chemical. Therefore it is important to investigate
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Comparative studies on the cytotoxic effects induced by iron oxide nanoparticles in cancerous and noncancerous human lung cells subjected to an alternating magnetic field Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-07 Monika Ruzycka-Ayoush, Kamil Sobczak, Ireneusz P. Grudzinski
The cytotoxic effects of water-based ferrofluids composed of iron oxide nanoparticles, including magnetite (Fe3O4) and maghemite (γ-Fe2O3), ranging from 15 to 100 nm, were examined on various lung cancer cells including adenocarcinomic human alveolar basal epithelial cells (A549), nonsmall lung squamous cell carcinoma (H1703), small cell lung cancer cells (DMS 114), and normal bronchial epithelial
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Changes in attachment and metabolic activity of rat neonatal cardiomyocytes and nonmyocytes caused by Macrovipera lebetina obtusa venom Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-06 Hovhannes Arestakesyan, Narine LeFevre, Nikki Posnack, Arni Sarian, Vahan Grigoryan, Naira Ayvazyan, Armen Voskanyan, Narine Sarvazyan, Zaruhi Karabekian
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Betulinic acid inhibits the proliferation of human laryngeal carcinoma cells through reactive oxygen species-mediate mitochondrial apoptotic pathway Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-05 Huamin Zhang, Mengru Zhou, Chongtao Ye, Jiahao Qin, Xuan Lu, Chunyan Wang, Xude Wang, Xuejun Jin
Betulinic acid (BA), a natural pentacyclic triterpene, was extracted from the white birch tree, Triphyophyllum peltatum and the jujube tree. In a variety of human cancer cell lines, this substance displays anticancer properties. In this study, we examined how BA works to inhibit human laryngeal cancer growth. We discovered that BA minimally exhibited cytotoxicity in normal cells (human normal cell
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The mechanism of anticancer effects of some pyrrolopyrimidine derivatives on HT-29 human colon cancer cells Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-05 Mustafa Ergul, Zuhal Kilic-Kurt, Yeliz Aka, Ozgur Kutuk, Zeynep Deniz Sahin-Inan
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Nilotinib exhibits less toxicity than imatinib and influences the immune state by modulating iNOS, p-p38 and p-JNK in LPS/IFN gamma-activated macrophages Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-05 Arzu Zeynep Karabay, Tulin Ozkan, Asli Koc, Yalda Hekmatshoar, A. Selen Gurkan-Alp, Asuman Sunguroglu
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Newly synthesized derivatives with a thiosemicarbazide group reduce the viability of cancer cell lines. Acute toxicity assessment in Zebrafish (Danio rerio) early life stages Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-27 Łucja Justyna Walczak-Nowicka, Aleksandra Szopa, Monika Pitucha, Anna Serefko, Anna Pachuta-Stec, Kamil Pawłowski, Monika Gawrońska-Grzywacz, Joanna Lachowicz, Mariola Herbet
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METTL3 regulates FAM83D m6A modification to accelerate tumorigenesis of triple-negative breast cancer via the Wnt/β-catenin pathway Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-01 Xiaodong Yu, Yaxun Li, Fanshuang Kong, Qun Xu
N6-methyladenosine (m6A) modification, the most abundant methylation modification on eukaryotic mRNAs, was implicated in the tumourigenesis. This study aimed to explore the role of methyltransferase like 3 (METTL3) in triple-negative breast cancer progression and its underlying mechanisms. FAM83D was markedly elevated in triple-negative breast cancer tissues and cells, and high expression of FAM83D
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A multiparametric and orthogonal approach indicates low toxicity for zein nanoparticles in a repellent formulation Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-01 Tuanny Leite Almeida, Aline Francisca Moreira, Jhones Luiz de Oliveira, Carolina Barbara Rogerio, Samara Flamini Kiihl, Leonardo Fernandes Fraceto, Marcelo Bispo de Jesus
The incidence of viruses such as Zika, Dengue, and Chikungunya affects human health worldwide, and insect repellents are recommended for individual protection. Formulations incorporating nanotechnology should be carefully assessed for toxicity, particularly regarding the security levels established for human health and the environment. This study evaluates the cytotoxicity of a repellent formulation
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Inhibitory effects of the main metabolites of Apatinib on CYP450 isozymes in human and rat liver microsomes Toxicol. In Vitro (IF 3.2) Pub Date : 2023-12-01 Ni-hong Pang, Ren-ai Xu, Lian-guo Chen, Zhe Chen, Guo-xin Hu, Bo-wen Zhang
Purpose The inhibitory effect of Apatinib on cytochrome P450 (CYP450) enzymes has been studied. However, it is unknown whether the inhibition is related to the major metabolites, M1–1, M1–2 and M1–6. Methods A 5-in-1 cocktail system composed of CYP2B6/Cyp2b1, CYP2C9/Cyp2c11, CYP2E1/Cyp2e1, CYP2D6/Cyp2d1 and CYP3A/Cyp3a2 was used in this study. Firstly, the effects of APA and its main metabolites on
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Electromagnetic field (50 Hz) enhance metabolic potential and induce adaptive/reprogramming response mediated by the increase of N6-methyladenosine RNA methylation in adipose-derived mesenchymal stem cells in vitro Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-29 Anna Sendera, Jagoda Adamczyk-Grochala, Barbara Pikuła, Marian Cholewa, Agnieszka Banaś-Ząbczyk
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Chemotherapeutic effect of baicalein/epirubicin combination against liver cell carcinoma in-vitro: Inducing apoptosis and autophagy Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-29 Ghada Mohammad Al-Ashmawy, Suzy Abd El-Hakeem El-Sherbeni, Dina Adam Ali, Mariam Ali Abo-Saif
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Phototoxic action of meloxicam contributes to dysregulation of redox homeostasis in normal human skin cells – Molecular and biochemical analysis of antioxidant enzymes in melanocytes and fibroblasts Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-29 Marta Karkoszka, Jakub Rok, Justyna Kowalska, Zuzanna Rzepka, Klaudia Banach, Dorota Wrześniok
The phototoxic effect of meloxicam (MLX) raises the question of the effect of the drug on the redox homeostasis of normal human skin cells. The main objective of the study was to analyze the effect of MLX and/or UVA radiation (UVAR) on the redox homeostasis of human normal skin cells – melanocytes and fibroblasts. MLX was found to affect the activity and expression of enzymes of the antioxidant system
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Pyruvate dehydrogenase is a potential mitochondrial off-target for gentamicin based on in silico predictions and in vitro inhibition studies Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-28 Charlotte A. Hoogstraten, Jan B. Koenderink, Carolijn E. van Straaten, Tom Scheer-Weijers, Jan A.M. Smeitink, Tom J.J. Schirris, Frans G.M. Russel
During the drug development process, organ toxicity leads to an estimated failure of one-third of novel chemical entities. Drug-induced toxicity is increasingly associated with mitochondrial dysfunction, but identifying the underlying molecular mechanisms remains a challenge. Computational modeling techniques have proven to be a good tool in searching for drug off-targets. Here, we aimed to identify
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DEHP and DINP accelerate aging effects in male and female of Drosophila melanogaster depend on AKT/FOXO pathway Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-26 Xudong Liu, Lulu Gao, Xian Li, Yang Liu, Xiaofan Lou, Mingsheng Yang, Weidong Wu, Xiaomeng Liu
Phthalates are commonly used as plasticizers. Numerous studies have focused on endocrine, reproductive, and developmental toxicity of phthalates exposure to male organisms. In recent years, some studies looking into the aging effects of phthalates exposure in D. melanogaster showed discrepant results. In this study, we compared the different concentrations of Di(2-ethylhexyl) phthalate (DEHP) and di-isononyl
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Predictive toxicological effects of Artemisia absinthium essential oil on hepatic stellate cells Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-22 I.I. Barreto, L.R. Gonçalves, A.F. Corrêa, M.A. Marin-Morales, K.C.M. Moraes
Medicinal plants are important worldwide, considering their properties for treating diseases; however, few studies have evaluated their toxicological potential. Among them, Artemisia absinthium is frequently used to treat liver diseases, because its essential oil has several popular therapeutic properties. Based on this information, in the present study, we investigated molecular connectors of physiological
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Evaluation of the acute toxicity of ellagic acid and gallic acid incorporated in Poloxamer407® gel, in Zophobas morio larvae Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-20 Jamilly Erica Sousa Campelo, Matheus Oliveira do Nascimento, André Luis Menezes Carvalho, Hayana Sara Pereira Santos, José Otavio Carvalho Sena de Almeida, Michel Mualem de Moraes Alves, Daniel Dias Rufino Arcanjo, José Moreira Tavares Neto, Maria Christina Sanches Muratori, Amilton Paulo Raposo Costa
Gallic acid (GA) has antioxidant, anti-inflammatory and antimicrobial properties, while ellagic acid (EA) demonstrates anticancer, antiviral and photoprotective activity. In this study, the combination of these substances incorporated into a poloxamer gel was tested to verify the individual effect of the substances, in addition to taking advantage of a probable complementary effect, aiming to provide
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Applications of Premature Chromosome Condensation technique for genetic analysis Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-19 C.H. Anjali, Ravi Maddaly
Cytogenetic techniques are used to detect aberrations in the genetic material and such techniques have a wide range of applications including for disease diagnosis, drug discovery and for the detection and quantification of mutagenic exposures. Although different types of cytogenetic techniques are in use, the Premature Chromosome Condensation (PCC) is one which is unique by virtue of it not requiring
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Life cell imaging of amiodarone sequestration into lamellar bodies of alveolar type II cells Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-18 Thomas Haller, Alexander Jesacher, Alberto Hidalgo, Christina Schmidt
Amiodarone is widely used to treat cardiac arrhythmias and is very effective in preventing these disorders. However, its use is limited by a wide range of adverse effects, mainly affecting the lungs, and ranging from mild shortness of breath to pulmonary fibrosis. Amiodarone has been shown to accumulate strongly in lung tissue, exceeding its plasma concentration by a hundredfold. However, the site
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Skin barrier function for regulatory skin absorption tests and effects on testosterone and sucrose absorption Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-17 Thomas Brackin, Heather Gayes, Ian Johnson, David Fox, Clive Roper
In vitro absorption through human skin is a critical component in the safety assessment of chemicals, crop protection products, consumer healthcare products and cosmetics. A barrier integrity assay is used to identify skin samples which are potentially damaged. A retrospective analysis of 9978 electrical resistance (ER) measurements generated in a single laboratory (DTL) over a 15-year period was performed
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Knockdown of JMJD3 ameliorates cigarette smoke extract-triggered bronchial epithelial cell injury via ACSL4-dependent ferroptosis Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-13 Ruijun Qin, Ping Wang, Lingzhi Li
Chronic obstructive pulmonary disease (COPD), a heterogeneity of chronic respiratory disease pattern, presents considerable prevalence and mortality. We aimed to explore the role and mechanisms of Jumonji domain-containing protein-3 (JMJD3) in COPD. The viability and JMJD3 expression in human bronchial epithelial cell line BEAS-2B were respectively assayed by CCK-8 assay and Western blot following
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Impacts of high-dose riboflavin on cytotoxicity, antioxidant, growth, reproductive gene expressions, and genotoxicity in the rainbow trout gonadal cells Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-07 Sevda Işık, Semra Çiçek
Riboflavin (vitamin B2 found in food) is a precursor of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which study as coenzymes for a variety of cellular processes including biosynthesis, homocysteine metabolism, detoxification, and various oxidation and reduction reactions. Although studies on the symptoms resulting from riboflavin deficiency are intense, studies on the effects
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3D multi-cell-type liver organoids: A new model of non-alcoholic fatty liver disease for drug safety assessments Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-10 J. Bronsard, C. Savary, J. Massart, R. Viel, L. Moutaux, D. Catheline, V. Rioux, B. Clement, A. Corlu, B. Fromenty, P.J. Ferron
The development of in vitro models that recapitulate critical liver functions is essential for accurate assessments of drug toxicity. Although liver organoids can be used for drug discovery and toxicology, they are limited by (i) the lack of expression and activity of xenobiotic-metabolizing enzymes, and (ii) the difficulty of mimicking non-alcoholic fatty liver disease (NAFLD, which influences the
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Cucurbitacin B and erastin co-treatment synergistically induced ferroptosis in breast cancer cells via altered iron-regulating proteins and lipid peroxidation Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-11 Filiz Bakar-Ates, Erva Ozkan
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Overdose with the anesthetic propofol causes hematological cytotoxicity and immune cell alteration in an experimental ex vivo whole blood culture model Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-05 Chung-Hsi Hsing, Yu-Ping Hung, Ming-Chung Lin, Chia-Ling Chen, Yung-Ting Wang, Po-Chun Tseng, Rahmat Dani Satria, Chiou-Feng Lin
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Assessment of the utility of the novel Phenion® full thickness human skin model for detecting the skin irritation potential of antimicrobial cleaning products Toxicol. In Vitro (IF 3.2) Pub Date : 2023-11-04 Kathryn Page, Walter Westerink, Kristie Sullivan, Thomas McDonald, Clive Roper
The skin is a potential route of exposure to antimicrobial cleaning products (ACP). Skin irritation, reversible damage to the skin, is an endpoint for protecting consumers and operators accidently exposed to these complex mixtures. To assess skin irritation of 24 ACP formulations, a new protocol was developed and adapted from OECD Test Guideline No. 439 with EpiDerm™ (epidermis model) replaced by Phenion®
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MLtox, online phototoxicity prediction webpage Toxicol. In Vitro (IF 3.2) Pub Date : 2023-10-24 Matej Halinkovič, Kateřina Mušková, Tibor Sloboda, Marek Lepáček, Helena Kanďárová, Michal Ries, Marta Šoltésová Prnová
Phototoxicity, sometimes in the literature referred to as photo-irritation, is a chemically induced reaction requiring light. While it is generally accepted that phototoxicity testing can be performed in the majority of cases in vitro (i.e. without the use of experimental animals), these tests may sometimes provide contradictory predictions. Understanding the mechanisms of initiating events based on