-
Perspectives on label-free microscopy of heterogeneous and dynamic biological systems J. Biomed. Opt. (IF 3.5) Pub Date : 2024-02-01 Dan L. Pham, Amani A. Gillette, Jeremiah Riendeau, Kasia Wiech, Emmanuel Contreras Guzman, Rupsa Datta, Melissa C. Skala
SignificanceAdvancements in label-free microscopy could provide real-time, non-invasive imaging with unique sources of contrast and automated standardized analysis to characterize heterogeneous and dynamic biological processes. These tools would overcome challenges with widely used methods that are destructive (e.g., histology, flow cytometry) or lack cellular resolution (e.g., plate-based assays,
-
Study on anisotropy orientation due to well-ordered fibrous biological microstructures J. Biomed. Opt. (IF 3.5) Pub Date : 2024-02-01 Zhidi Liu, Jiawei Song, Qiqi Fu, Nan Zeng, Hui Ma
SignificanceMost biological fibrous tissues have anisotropic optical characteristics, which originate from scattering by their fibrous microstructures and birefringence of biological macromolecules. The orientation-related anisotropic interpretation is of great value in biological tissue characterization and pathological diagnosis.AimWe focus on intrinsic birefringence and form birefringence in biological
-
Mueller matrix-based characterization of cervical tissue sections: a quantitative comparison of polar and differential decomposition methods J. Biomed. Opt. (IF 3.5) Pub Date : 2024-02-01 Nishkarsh Kumar, Jeeban Kumar Nayak, Asima Pradhan, Nirmalya Ghosh
SignificanceQuantitative optical polarimetry has received considerable recent attention owing to its potential for being an efficient diagnosis and characterizing tool with potential applications in biomedical research and various other disciplines. In this regard, it is crucial to validate various Mueller matrix (MM) decomposition methods, which are utilized to extract and quantify the intrinsic individual
-
Speculum-free portable preterm imaging system J. Biomed. Opt. (IF 3.5) Pub Date : 2024-01-01 Tananant Boonya-ananta, Mariacarla Gonzalez, Ajmal Ajmal, Vinh Nguyen Du Le, Edward DeHoog, Michael J. Paidas, Arumugam Jayakumar, Jessica C. Ramella-Roman
SignificancePreterm birth is defined as a birth before 37 weeks of gestation and is one of the leading contributors to infant mortality rates globally. Premature birth can lead to life-long developmental impairment for the child. Unfortunately, there is a significant lack of tools to diagnose preterm birth risk, which limits patient care and the development of new therapies.AimTo develop a speculum-free
-
Analysis of polarization features of human breast cancer tissue by Mueller matrix visualization J. Biomed. Opt. (IF 3.5) Pub Date : 2024-01-01 Thi-Thu-Hien Pham, Thao-Ngan Ngoc Quach, Quoc-Hoang-Quyen Vo
SignificanceBreast cancer ranks second in the world in terms of the number of women diagnosed. Effective methods for its early-stage detection are critical for facilitating timely intervention and lowering the mortality rate.AimPolarimetry provides much useful information on the structural properties of breast cancer tissue samples and is a valuable diagnostic tool. The present study classifies human
-
Evaluating backscattering polarized light imaging microstructural mapping capabilities through neural tissue and analogous phantom imaging J. Biomed. Opt. (IF 3.5) Pub Date : 2023-12-01 Justina Bonaventura, Kellys Morara, Rhea Carlson, Courtney Comrie, AnneLeigh Twer, Elizabeth Hutchinson, Travis W. Sawyer
SignificanceKnowledge of fiber microstructure and orientation in the brain is critical for many applications. Polarized light imaging (PLI) has been shown to have potential for better understanding neural fiber microstructure and directionality due to the anisotropy in myelin sheaths surrounding nerve fibers of the brain. Continuing to advance backscattering based PLI systems could provide a valuable
-
Quantitative classification of melasma with photoacoustic microscopy: a pilot study J. Biomed. Opt. (IF 3.5) Pub Date : 2023-11-01 Zhiyang Wang, Yuying Chen, Shu Pan, Wuyu Zhang, Ziwei Guo, Yuzhi Wang, Sihua Yang
SignificanceThe classification of melasma is critical for correct clinical diagnosis, treatment selection, and postoperative measures. However, preoperative quantitative determination of melasma type remains challenging using conventional Wood’s lamp and optical dermoscopy techniques.AimUsing photoacoustic microscopy (PAM) to simultaneously obtain the two diagnostic indicators of melanin and blood
-
Machine learning based local recurrence prediction in colorectal cancer using polarized light imaging J. Biomed. Opt. (IF 3.5) Pub Date : 2023-11-01 Anamitra Majumdar, Jigar Lad, Kseniia Tumanova, Stefano Serra, Fayez Quereshy, Mohammadali Khorasani, Alex Vitkin
SignificanceCurrent treatment for stage III colorectal cancer (CRC) patients involves surgery that may not be sufficient in many cases, requiring additional adjuvant systemic therapy. Identification of this latter cohort that is likely to recur following surgery is key to better personalized therapy selection, but there is a lack of proper quantitative assessment tools for potential clinical adoption
-
Exploring the evolution of circular polarized light backscattered from turbid tissue-like disperse medium utilizing generalized Monte Carlo modeling approach with a combined use of Jones and Stokes–Mueller formalisms J. Biomed. Opt. (IF 3.5) Pub Date : 2023-11-01 Ivan Lopushenko, Oleksii Sieryi, Alexander Bykov, Igor Meglinski
SignificancePhase retardation of circularly polarized light (CPL), backscattered by biological tissue, is used extensively for quantitative evaluation of cervical intraepithelial neoplasia, presence of senile Alzheimer’s plaques, and characterization of biotissues with optical anisotropy. The Stokes polarimetry and Mueller matrix approaches demonstrate high potential in definitive non-invasive cancer
-
Incremental residual polarization caused by aging in human skin J. Biomed. Opt. (IF 3.5) Pub Date : 2023-11-01 Viktor Dremin, Elena Zharkikh, Ivan Lopushenko, Zbignevs Marcinkevics, Alexander Bykov, Igor Meglinski
SignificanceThe study of the effect of aging on the optical properties of biological tissues, in particular polarization, is important in the development of new diagnostic approaches.AimThis work aims to provide a comprehensive analysis of the factors and mechanisms that contribute to the alteration of skin polarization properties caused by aging, using polarization-sensitive hyperspectral imaging
-
Deep learning in vivo catheter tip locations for photoacoustic-guided cardiac interventions J. Biomed. Opt. (IF 3.5) Pub Date : 2023-11-01 Mardava R. Gubbi, Fabrizio Assis, Jonathan Chrispin, Muyinatu A. Lediju Bell
SignificanceInterventional cardiac procedures often require ionizing radiation to guide cardiac catheters to the heart. To reduce the associated risks of ionizing radiation, photoacoustic imaging can potentially be combined with robotic visual servoing, with initial demonstrations requiring segmentation of catheter tips. However, typical segmentation algorithms applied to conventional image formation
-
Measuring and imaging of transcutaneous bilirubin, hemoglobin, and melanin based on diffuse reflectance spectroscopy. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-31 Masafumi Minakawa,Md Abdul Wares,Kazuya Nakano,Hideaki Haneishi,Yoshihisa Aizu,Yoshio Hayasaki,Tetsuo Ikeda,Hajime Nagahara,Izumi Nishidate
Significance Evaluation of biological chromophore levels is useful for detection of various skin diseases, including cancer, monitoring of health status and tissue metabolism, and assessment of clinical and physiological vascular functions. Clinically, it is useful to assess multiple different chromophores in vivo with a single technique or instrument. Aim To investigate the possibility of estimating
-
Comparison of minimal detectable protoporphyrin IX concentrations with a loupe device and conventional 5-ALA fluorescence microscopy: an experimental study. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-31 Mario Mischkulnig,Denise Traxler,Lisa I Wadiura,Alexandra Lang,Matthias Millesi,Barbara Kiesel,Georg Widhalm
Significance The 5-aminolevulinic acid (5-ALA) fluorescence technique is now widely applied for intraoperative visualization of specific central nervous system (CNS) tumors. Previous technical implementations of this technique have relied on specifically modified surgical microscopes to visualize intratumoral fluorescent protoporphyrin (PpIX). While this approach evidently allows for reliable intraoperative
-
Observation and density estimation of a large number of skin capillaries using wide-field portable video capillaroscopy and semantic segmentation. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-24 Baku Takimoto,Kotatsu Bito,Sayaka Hari,Hiroyuki Taguchi,Hideaki Haneishi
Significance Skin capillaries are non-invasively observable; their structure and blood flow can reflect tissue and systemic conditions. Quantitative analysis of video-capillaroscopy images yields novel diagnostic methods. Because the capillary structure is heterogeneous, analyzing more capillaries can increase the evaluation reliability. Aim We developed a system that can observe and quantify numerous
-
Deep-learning-based cross-modality translation from Stokes image to bright-field contrast. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-20 Shilong Wei,Lu Si,Tongyu Huang,Shan Du,Yue Yao,Yang Dong,Hui Ma
Significance Mueller matrix (MM) microscopy has proven to be a powerful tool for probing microstructural characteristics of biological samples down to subwavelength scale. However, in clinical practice, doctors usually rely on bright-field microscopy images of stained tissue slides to identify characteristic features of specific diseases and make accurate diagnosis. Cross-modality translation based
-
ArthroNavi framework: stereo endoscope-guided instrument localization for arthroscopic minimally invasive surgeries. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-14 Zhongjie Long,Yongting Chi,Xiaotong Yu,Zhouxiang Jiang,Dejin Yang
Significance As an example of a minimally invasive arthroscopic surgical procedure, arthroscopic osteochondral autograft transplantation (OAT) is a common option for repairing focal cartilage defects in the knee joints. Arthroscopic OAT offers considerable benefits to patients, such as less post-operative pain and shorter hospital stays. However, performing OAT arthroscopically is an extremely demanding
-
Quantification of microvascular change of retinal degeneration in Royal College of Surgeons rats using high-resolution spectral domain optical coherence tomography angiography. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-13 Zhen-Jie Zhang,You-Ren Wu,Yueh Chien,Yang Chen,Shih-Hwa Chiou,Shih-Jen Chen,Jia-Pu Syu,Wen-Chuan Kuo
Significance For research on retinitis pigmentosa in humans, the Royal College of Surgeons (RCS) rat is commonly used as the primary animal model since the disease process is similar. Therefore, it is necessary to understand how the disease develops and determine whether the treatment is effective. Aim In this study, structural and microvascular change of retinal degeneration in RCS rats was assessed
-
scatterBrains: an open database of human head models and companion optode locations for realistic Monte Carlo photon simulations. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-05 Melissa M Wu,Roarke Horstmeyer,Stefan A Carp
Significance Monte Carlo (MC) simulations are currently the gold standard in the near-infrared and diffuse correlation spectroscopy (NIRS/DCS) communities for generating light transport paths through tissue. However, realistic and diverse models that capture complex tissue layers are not easily available to all; moreover, manually placing optodes on such models can be tedious and time consuming. Such
-
Cascaded domain multiphoton spatial frequency modulation imaging. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-04 Daniel Scarbrough,Anna Thomas,Jeff Field,Randy Bartels,Jeff Squier
Significance Multiphoton microscopy is a powerful imaging tool for biomedical applications. A variety of techniques and respective benefits exist for multiphoton microscopy, but an enhanced resolution is especially desired. Additionally multiphoton microscopy requires ultrafast pulses for excitation, so optimization of the pulse duration at the sample is critical for strong signals. Aim We aim to perform
-
Noise insensitive volumetric fusion method for enhanced photoacoustic microscopy. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-04 Sihang Li,Hao Wu,Hongyu Zhang,Zhipeng Zhang,Qiugen Liu,Xianlin Song
Significance Photoacoustic imaging is an emerging imaging modality that combines the high contrast of optical imaging and the high penetration of acoustic imaging. However, the strong focusing of the laser beam in optical-resolution photoacoustic microscopy (OR-PAM) leads to a limited depth-of-field (DoF). Aim Here, a volumetric photoacoustic information fusion method was proposed to achieve large
-
Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-01 Wei-Hong Yeo, Cheng Sun, Hao F. Zhang
SignificanceThe dual-wedge prism (DWP)-based spectroscopic single-molecule localization microscopy (sSMLM) system offers improved localization precision and adjustable spectral or localization performance, but its nonlinear spectral dispersion presents a challenge. A systematic method can help understand the challenges and thereafter optimize the DWP system’s performance by customizing the system parameters
-
Review of low-cost light sources and miniaturized designs in photoacoustic microscopy J. Biomed. Opt. (IF 3.5) Pub Date : 2023-10-01 Bingxin Huang, Terence T. W. Wong
SignificancePhotoacoustic microscopy (PAM) is a promising imaging technique to provide structural, functional, and molecular information for preclinical and clinical studies. However, expensive and bulky lasers and motorized stages have limited the broad applications of conventional PAM systems. A recent trend is to use low-cost light sources and miniaturized designs to develop a compact PAM system
-
Influence of hematoxylin and eosin staining on linear birefringence measurement of fibrous tissue structures in polarization microscopy. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-30 Liangyu Deng,Chunyan Chen,Wenxi Yu,Conghui Shao,Zan Shen,Yonggang Wang,Chao He,Hongtao Li,Zhiyan Liu,Honghui He,Hui Ma
Significance For microscopic polarization imaging of tissue slices, two types of samples are often prepared: one unstained tissue section for polarization imaging to avoid possible influence from staining dyes quantitatively and one hematoxylin-eosin (H&E) stained adjacent tissue section for histological diagnosis and structural feature identification. However, this sample preparation strategy requires
-
Determination of tissue oxygen saturation by diffuse reflectance spectroscopy. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-28 Laura Lucía Sánchez-Ramos,Beatriz Morales-Cruzado,Francisco Gerardo Pérez-Gutiérrez
Significance Tissue oxygenation is a parameter that allows for determining the health status of human beings. In diabetic patients, it is particularly important to evaluate this parameter as an indicator of microcirculatory problems in the extremities. Aim We aim to obtain tissue oxygen saturation from diffuse reflectance measurements. Approach A computational algorithm to automate the methodology
-
Assessment of open-field fluorescence guided surgery systems: implementing a standardized method for characterization and comparison. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-21 Marien I Ochoa,Alberto Ruiz,Ethan LaRochelle,Matthew Reed,Eren Berber,George Poultsides,Brian W Pogue
Significance Fluorescence guided surgery (FGS) has demonstrated improvements in decision making and patient outcomes for a wide range of surgical procedures. Not only can FGS systems provide a higher level of structural perfusion accuracy in tissue reconstruction cases but they can also serve for real-time functional characterization. Multiple FGS devices have been Food and Drug administration (FDA)
-
Fluorescence-guided surgical system using holographic display: from phantom studies to canine patients. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-20 Mebin B George,Benjamin Lew,Zuodong Liang,Steven Blair,Zhongmin Zhu,Nan Cui,Jamie Ludwig,Mohamed Zayed,Laura Selmic,Viktor Gruev
Significance Holographic display technology is a promising area of research that can lead to significant advancements in cancer surgery. We present the benefits of combining bioinspired multispectral imaging technology with holographic goggles for fluorescence-guided cancer surgery. Through a series of experiments with 43D-printed phantoms, small animal models of cancer, and surgeries on canine patients
-
Multiexposure laser speckle contrast analysis system calibration limited by perfusion-dependent scattering on the skin. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-15 Tamás Smausz,Bence Kondász
Significance Application of multiexposure speckle contrast imaging (MESI) methods for perfusion measurements can correct for the contribution of static scattering of the skin, at the expense of reduced temporal resolution as compared to classical single-exposure methods. Persistence of tissue scattering properties during the measurements could allow for an initial calibration and enhancement of the
-
Integration of cellular-resolution optical coherence tomography and Raman spectroscopy for discrimination of skin cancer cells with machine learning. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-14 Cian You,Jui-Yun Yi,Ting-Wei Hsu,Sheng-Lung Huang
Significance An integrated cellular-resolution optical coherence tomography (OCT) module with near-infrared Raman spectroscopy was developed on the discrimination of various skin cancer cells and normal cells. Micron-level three-dimensional (3D) spatial resolution and the spectroscopic capability on chemical component determination can be obtained simultaneously. Aim We experimentally verified the
-
Wide field-of-view fluorescence imaging for organ-level lineage tracing of rare intestinal stem cell populations. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-13 Noelle Daigle,Suzann Duan,Heyu Song,Natzem Lima,Ricky Sontz,Juanita L Merchant,Travis W Sawyer
Significance Lineage tracing using fluorescent reporters is a common tool for monitoring the expression of genes and transcription factors in stem cell populations and their progeny. The zinc-binding protein 89 (ZBP-89/Zfp148 mouse gene) is a transcription factor that plays a role in gastrointestinal (GI) stem cell maintenance and cellular differentiation and has been linked to the progression of colon
-
Impact of corpus callosum fiber tract crossing on polarimetric images of human brain histological sections: ex vivo studies in transmission configuration. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-12 Deyan Ivanov,Lu Si,Leonard Felger,Theoni Maragkou,Philippe Schucht,Marie-Claire Schanne-Klein,Hui Ma,Razvigor Ossikovski,Tatiana Novikova
Significance Imaging Mueller polarimetry is capable to trace in-plane orientation of brain fiber tracts by detecting the optical anisotropy of white matter of healthy brain. Brain tumor cells grow chaotically and destroy this anisotropy. Hence, the drop in scalar retardance values and randomization of the azimuth of the optical axis could serve as the optical marker for brain tumor zone delineation
-
Multifocal acoustic radiation force-based reverberant optical coherence elastography for evaluation of ocular globe biomechanical properties. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-11 Taye Mekonnen,Christian Zevallos-Delgado,Manmohan Singh,Salavat R Aglyamov,Kirill V Larin
Significance Quantifying the biomechanical properties of the whole eye globe can provide a comprehensive understanding of the interactions among interconnected ocular components during dynamic physiological processes. By doing so, clinicians and researchers can gain valuable insights into the mechanisms underlying ocular diseases, such as glaucoma, and design interventions tailored to each patient's
-
Compressive hyperspectral microscopy for cancer detection. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-09 Yaniv Oiknine,Marwan Abuleil,Eugene Brozgol,Isaac Y August,Iris Barshack,Ibrahim Abdulhalim,Yuval Garini,Adrian Stern
Significance Hyperspectral microscopy grants the ability to characterize unique properties of tissues based on their spectral fingerprint. The ability to label and measure multiple molecular probes simultaneously provides pathologists and oncologists with a powerful tool to enhance accurate diagnostic and prognostic decisions. As the pathological workload grows, having an objective tool that provides
-
Subsecond lung cancer detection within a heterogeneous background of normal and benign tissue using single-point Raman spectroscopy. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-09 Frédéric Leblond,Frédérick Dallaire,Trang Tran,Rajeev Yadav,Kelly Aubertin,Eric Goudie,Philippe Romeo,Christopher Kent,Charles Leduc,Moishe Liberman
Significance Lung cancer is the most frequently diagnosed cancer overall and the deadliest cancer in North America. Early diagnosis through current bronchoscopy techniques is limited by poor diagnostic yield and low specificity, especially for lesions located in peripheral pulmonary locations. Even with the emergence of robotic-assisted platforms, bronchoscopy diagnostic yields remain below 80%. Aim
-
Spectroscopic optical coherence tomography at 1200 nm for lipid detection. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-09 Vivek Kuttippurath,Nuria Slijkhuis,Shengnan Liu,Gijs van Soest
Significance Spectroscopic analysis of optical coherence tomography (OCT) data can yield added information about the sample's chemical composition, along with high-resolution images. Typical commercial OCT systems operate at wavelengths that may not be optimal for identifying lipid-containing samples based on absorption features. Aim The main aim of this study was to develop a 1200 nm spectroscopic
-
Special Section Guest Editorial: Short Wave Infrared Techniques and Applications in Biomedical Optics. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-09 Daniel Fried,Mark Pierce,Darren Roblyer
The editorial introduces the JBO Special Section on Short Wave Infrared Techniques and Applications in Biomedical Optics.
-
Analysis of structural effects of sickle cell disease on brain vasculature of mice using three-dimensional quantitative phase imaging. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-09 Caroline Filan,Hannah Song,Manu O Platt,Francisco E Robles
Significance Although the molecular origins of sickle cell disease (SCD) have been extensively studied, the effects of SCD on the vasculature-which can influence blood clotting mechanisms, pain crises, and strokes-are not well understood. Improving this understanding can yield insight into the mechanisms and wide-ranging effects of this devastating disease. Aim We aim to demonstrate the ability of
-
Spatial analysis of polarimetric images to enhance near-surface sampling sensitivity: feasibility in demineralized teeth and other tissue-like media. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-09 Michael D Singh,Lothar Lilge,Alex Vitkin
Significance Early tooth demineralization may be detectable through spatial analysis of polarized light images as demonstrated in this study. This may also prove useful in the early detection of epithelial tumors that comprise the majority of the cancer burden worldwide. Aim The spatial properties of polarized light images have not been greatly exploited in biomedicine to improve sensitivity to superficial
-
Frameworks of wavelength selection in diffuse reflectance spectroscopy for tissue differentiation in orthopedic surgery J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-01 Celina L. Li, Carl J. Fisher, Katarzyna Komolibus, Konstantin Grygoryev, Huihui Lu, Ray Burke, Andrea Visentin, Stefan Andersson-Engels
SignificanceWavelength selection from a large diffuse reflectance spectroscopy (DRS) dataset enables removal of spectral multicollinearity and thus leads to improved understanding of the feature domain. Feature selection (FS) frameworks are essential to discover the optimal wavelengths for tissue differentiation in DRS-based measurements, which can facilitate the development of compact multispectral
-
Projection mapping system for laser speckle contrast image: feasibility study for clinical application. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-04 Subin Park,Insun Yeum,Donghwan Ko,Byungjo Jung
Significance Laser speckle contrast images (LSCIs) have been utilized to monitor blood flow perfusion. However, they have conventionally been observed on monitor screens, resulting in potential spatial mismatching between the imaging region of interest (IROI) and monitor screen. Aim This study proposes a projection mapping (PM) system for LSCIs (PMS_LSCI) that projects LSCIs to directly observe the
-
Optical absorption spectra and corresponding in vivo photoacoustic visualization of exposed peripheral nerves. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-04 Michelle T Graham,Arunima Sharma,William M Padovano,Visakha Suresh,Arlene Chiu,Susanna M Thon,Sami Tuffaha,Muyinatu A Lediju Bell
Significance Multispectral photoacoustic imaging has the potential to identify lipid-rich, myelinated nerve tissue in an interventional or surgical setting (e.g., to guide intraoperative decisions when exposing a nerve during reconstructive surgery by limiting operations to nerves needing repair, with no impact to healthy or regenerating nerves). Lipids have two optical absorption peaks within the
-
Perspective on the optics of medical imaging J. Biomed. Opt. (IF 3.5) Pub Date : 2023-09-01 Brian W. Pogue
SignificanceMedical imaging is very commonly described as synonymous with radiological imaging, yet optical imaging devices are widely distributed throughout many medical specialties. This delocalized nature of the technology reduces visibility and dominance as a cohesive medical technology sector.AimIndicators of impact of medical optical systems were examined and compared to the radiology technology
-
Special Section Guest Editorial: Seeing Inside Tissue with Optical Molecular Probes. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-30 Nada N Boustany,Mark Niedre,Manojit Pramanik
The editorial introduces the Special Section on Seeing Inside Tissue with Optical Molecular Probes.
-
Customizable optical tissue phantom platform for characterization of fluorescence imaging device sensitivity. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-30 Christopher Gibson,Arcturus Phoon,Ralph S DaCosta
Significance Optical tissue phantoms serve as inanimate and stable reference materials used to calibrate, characterize, standardize, and test biomedical imaging instruments. Although various types of solid tissue phantoms have been described in the literature, current phantom models are limited in that they do not have a depth feature that can be adjusted in real-time, they cannot be adapted to other
-
Spatial averaging method based on adaptive weight for imaging photoplethysmography. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-30 JongSong Ryu,HyonSam Ryu,Shili Liang,SunChol Hong,Yueqi Lian,Zong Zheng
Significance Imaging photoplethysmography (iPPG) is a non-contact measuring technology for several physiological parameters reflecting personal health status without a special sensor. However, the pulse signal obtained using the iPPG usually is contaminated by various noises, and the intensity of the interesting pulse signal is relatively weak compared to the noises, emphasizing the necessity of obtaining
-
Smartphone-based dual radiometric fluorescence and white-light imager for quantification of protoporphyrin IX in skin. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-26 Alberto J Ruiz,Richard Allen,Mia K Giallorenzi,Kimberley S Samkoe,M Shane Chapman,Brian W Pogue
Significance The quantification of protoporphyrin IX (PpIX) in skin can be used to study photodynamic therapy (PDT) treatments, understand porphyrin mechanisms, and enhance preoperative mapping of non-melanoma skin cancers. Aim We aim to develop a smartphone-based imager for performing simultaneous radiometric fluorescence (FL) and white light (WL) imaging to study the baseline levels, accumulation
-
Two-stage classification strategy for breast cancer diagnosis using ultrasound-guided diffuse optical tomography and deep learning. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-26 Menghao Zhang,Shuying Li,Minghao Xue,Quing Zhu
Significance Ultrasound (US)-guided diffuse optical tomography (DOT) has demonstrated great potential for breast cancer diagnosis in which real-time or near real-time diagnosis with high accuracy is desired. Aim We aim to use US-guided DOT to achieve an automated, fast, and accurate classification of breast lesions. Approach We propose a two-stage classification strategy with deep learning. In the
-
Depth-dependent attenuation and backscattering characterization of optical coherence tomography by stationary iterative method. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-24 Yaning Wang,Shuwen Wei,Jin U Kang
Significance Extracting optical properties of tissue [e.g., the attenuation coefficient (μ) and the backscattering fraction] from the optical coherence tomography (OCT) images is a valuable tool for parametric imaging and related diagnostic applications. Previous attenuation estimation models depend on the assumption of the uniformity of the backscattering fraction (R) within layers or whole samples
-
Multivariate analysis of breast tissue using optical parameters extracted from a combined time-resolved fluorescence and diffuse reflectance system for tumor margin detection. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-23 Erica Dao,Gabriella Gohla,Phillip Williams,Peter Lovrics,Fares Badr,Qiyin Fang,Thomas J Farrell,Michael J Farquharson
Significance Breast conservation therapy is the preferred technique for treating primary breast cancers. However, breast tumor margins are hard to determine as tumor borders are often ill-defined. As such, there exists a need for a clinically compatible tumor margin detection system. Aim A combined time-resolved fluorescence and diffuse reflectance (TRF-DR) system has been developed to determine the
-
Evaluating the identification of the extent of gastric cancer by over-1000 nm near-infrared hyperspectral imaging using surgical specimens. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-22 Tomohiro Mitsui,Akino Mori,Toshihiro Takamatsu,Tomohiro Kadota,Konosuke Sato,Ryodai Fukushima,Kyohei Okubo,Masakazu Umezawa,Hiroshi Takemura,Hideo Yokota,Takeshi Kuwata,Takahiro Kinoshita,Hiroaki Ikematsu,Tomonori Yano,Shin Maeda,Kohei Soga
Significance Determining the extent of gastric cancer (GC) is necessary for evaluating the gastrectomy margin for GC. Additionally, determining the extent of the GC that is not exposed to the mucosal surface remains difficult. However, near-infrared (NIR) can penetrate mucosal tissues highly efficiently. Aim We investigated the ability of near-infrared hyperspectral imaging (NIR-HSI) to identify GC
-
Multiparameter interferometric polarization-enhanced imaging differentiates carcinoma in situ from inflammation of the bladder: an ex vivo study. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-10 Shuang Chang,Giovanna A Giannico,Ezekiel Haugen,Ali Jardaneh,Justin Baba,Anita Mahadevan-Jansen,Sam S Chang,Audrey K Bowden
Significance Successful differentiation of carcinoma in situ (CIS) from inflammation in the bladder is key to preventing unnecessary biopsies and enabling accurate therapeutic decisions. Current standard-of-care diagnostic imaging techniques lack the specificity needed to differentiate these states, leading to false positives. Aim We introduce multiparameter interferometric polarization-enhanced (MultiPIPE)
-
Optical imaging for early detection of cervical cancer: state of the art and perspectives. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-09 Alisha Rahaman,Arpitha Anantharaju,Karthika Jeyachandran,Repala Manideep,Uttam M Pal
Significance Cervical cancer is one of the major causes of death in females worldwide. HPV infection is the key cause of uncontrolled cell growth leading to cervical cancer. About 90% of cervical cancer is preventable because of the slow progression of the disease, giving a window of about 10 years for the precancerous lesion to be recognized and treated. Aim The present challenges for cervical cancer
-
Three-dimensional angular scattering simulations inform analysis of scattering from single cells. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-09 Kaitlin J Dunn,Andrew J Berger
Significance Organelle sizes, which are indicative of cellular status, have implications for drug development and immunology research. At the single cell level, such information could be used to study the heterogeneity of cell response to drugs or pathogens. Aim Angularly resolved elastic light scattering is known to be sensitive to changes in organelle size distribution. We developed a Mie theory-based
-
Porcine vocal fold elasticity evaluation using Brillouin spectroscopy. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-08 Vsevolod Cheburkanov,Ethan Keene,Jason Pipal,Michael Johns,Brian E Applegate,Vladislav V Yakovlev
Significance The vocal folds are critically important structures within the larynx which serve the essential functions of supporting the airway, preventing aspiration, and phonation. The vocal fold mucosa has a unique multilayered architecture whose layers have discrete viscoelastic properties facilitating sound production. Perturbations in these properties lead to voice loss. Currently, vocal fold
-
Review of optical reporters of radiation effects in vivo: tools to quantify improvements in radiation delivery technique. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-08 Jacob Sunnerberg,William S Thomas,Arthur Petusseau,Matthew S Reed,P Jack Hoopes,Brian W Pogue
Significance Radiation damage studies are used to optimize radiotherapy treatment techniques. Although biological indicators of damage are the best assays of effect, they are highly variable due to biological heterogeneity. The free radical radiochemistry can be assayed with optical reporters, allowing for high precision titration of techniques. Aim We examine the optical reporters of radiochemistry
-
Analyzing the influence of oblique incidence on quantitative backscattering tissue polarimetry: a pilot ex vivo study. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-07 Zheng Zhang,Conghui Shao,Honghui He,Chao He,Shaoxiong Liu,Hui Ma
Significance Among the available polarimetric techniques, backscattering Mueller matrix (MM) polarimetry provides a promising non-contact and quantitative tool for in vivo tissue detection and clinical diagnosis. To eliminate the surface reflection from the sample cost-effectively, the non-collinear backscattering MM imaging setup always has an oblique incidence. Meanwhile, for practical organ cavities
-
Noninvasive blood glucose sensing by secondary speckle pattern artificial intelligence analyses. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-01 Deep Pal,Amitesh Kumar,Nave Avraham,Yoram Eisenbach,Yevgeny Beiderman,Sergey Agdarov,Yafim Beiderman,Zeev Zalevsky
Significance Diabetes is a prevalent disease worldwide that can cause severe health problems. Accurate blood glucose detection is crucial for diabetes management, and noninvasive methods can be more convenient and less painful than traditional finger-prick methods. Aim We aim to report a noncontact speckle-based blood glucose measurement system that utilizes artificial intelligence (AI) data processing
-
Iterative prototyping based on lessons learned from the falloposcope in vivo pilot study experience J. Biomed. Opt. (IF 3.5) Pub Date : 2023-08-01 Andrew D. Rocha, William K. Drake, Photini F. Rice, Dilara J. Long, Hasina Shir, Ryan H. M. Walton, Mary N. Reed, Dominique Galvez, Taliah Gorman, John M. Heusinkveld, Jennifer K. Barton
SignificanceHigh grade serous ovarian cancer is the most deadly gynecological cancer, and it is now believed that most cases originate in the fallopian tubes (FTs). Early detection of ovarian cancer could double the 5-year survival rate compared with late-stage diagnosis. Autofluorescence imaging can detect serous-origin precancerous and cancerous lesions in ex vivo FT and ovaries with good sensitivity
-
Spectral correction of light emitting diodes enables accurate hydration ratio calculation using narrowband diffuse reflectance spectroscopy. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-07-31 Jesse H Lam,Jeonghun Kim,Kelsey J Tu,Dongbin Kim,Sehwan Kim
Significance Light emitting diodes (LEDs) are commonly utilized for tissue spectroscopy due to their small size, low cost, and simplicity. However, LEDs are often approximated as single-wavelength devices despite having relatively broad spectral bandwidths. When paired with photodiodes, the wavelength information of detected light cannot be resolved. This can result in errors during chromophore concentration
-
Cortical microvascular blood flow velocity mapping by combining dynamic light scattering optical coherence tomography and two-photon microscopy. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-07-21 Qi Pian,Mohammed Alfadhel,Jianbo Tang,Grace V Lee,Baoqiang Li,Buyin Fu,Yagmur Ayata,Mohammad Abbas Yaseen,David A Boas,Timothy W Secomb,Sava Sakadzic
Significance The accurate large-scale mapping of cerebral microvascular blood flow velocity is crucial for a better understanding of cerebral blood flow (CBF) regulation. Although optical imaging techniques enable both high-resolution microvascular angiography and fast absolute CBF velocity measurements in the mouse cortex, they usually require different imaging techniques with independent system configurations
-
Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow. J. Biomed. Opt. (IF 3.5) Pub Date : 2023-07-21 Tresa M Elias,Edward B Brown,Edward B Brown
Significance Multi-photon fluorescence recovery after photobleaching (MPFRAP) is a nonlinear microscopy technique used to measure the diffusion coefficient of fluorescently tagged molecules in solution. Previous MPFRAP fitting models calculate the diffusion coefficient in systems with diffusion or diffusion in laminar flow. Aim We propose an MPFRAP fitting model that accounts for shear stress in laminar