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A droplet vitrification cryopreservation protocol for conservation of hops (Humulus lupulus) genetic resources Cryobiology (IF 2.7) Pub Date : 2024-03-15 Era Vaidya Malhotra, Suresh Chand Mali, Shreya Sharma, Sangita Bansal
Hops ( L.) is essentially used in the brewing industry as it contributes to flavor, and aroma of beer. However, the genetic diversity of hops is increasingly threatened by diseases, environmental changes, and urbanization. Cryopreservation has emerged as a pivotal strategy for safeguarding and maintaining the genetic diversity of hops. The present work presents a comprehensive study on the cryopreservation
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l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa Cryobiology (IF 2.7) Pub Date : 2024-03-07 Paula Palacios, Gabriela Peláez, Manuel Soria, Silvana Méndez, Luis Galarza-Álvarez, Jesús Dorado, Julián Santiago-Moreno, Diego A. Galarza
- (LC) transports fatty acids to the mitochondria for energy production, reducing lipid availability for peroxidation through oxidation. This research examines the effect of LC supplementation to two skimmed milk-based extenders on the cryosurvival of chilled (5°C) and frozen-thawed Peruvian Paso horse spermatozoa .An initial experiment determined the optimal LC concentration (0, 1, 5, 10, 25, and
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Effects of different cryopreservation parameters on the differences between trypan blue and fluorescent SYTO 13/GelRed assays Cryobiology (IF 2.7) Pub Date : 2024-03-06 Elham Ashrafi, Dominic Sauvageau, JanetA.W. Elliott
Post-thaw cell viability assessment is very important in cryopreservation because it is the main assessment method used to optimize the cryopreservation protocols for each cell type; hence, having standardized accurate, quick, and reliable assays for post-thaw cell viability measurements is of utmost importance. The trypan blue exclusion assay and nucleic-acid-binding fluorescence-based assays are
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Improvement of survivability and developmental ability in vitrified rat oocytes Cryobiology (IF 2.7) Pub Date : 2024-03-05 Yuki Nakagawa, Takehito Kaneko
Oocyte cryopreservation is useful for human fertility treatment and strain preservation in both experimental and domestic animals. However, the embryonic development of vitrified rat oocytes was lower than that of vitrified embryos. To increase the viability of vitrified oocytes, intracellular ice formation during cooling and warming must be prevented. Rapid warming is important to prevent ice formation
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Cooling dynamics of droplets exposed to solid surface freezing and vitrification Cryobiology (IF 2.7) Pub Date : 2024-03-04 Dejia Liu, Harriëtte Oldenhof, Xing Luo, Tobias Braun, Harald Sieme, Willem F. Wolkers
Solid surface freezing or vitrification (SSF/SSV) can be done by depositing droplets of a sample, e.g., cells in a preservation solution, onto a pre-cooled metal surface. It is used to achieve higher cooling rates and concomitant higher cryosurvival rates compared to immersion of samples into liquid nitrogen. In this study, numerical simulations of SSF/SSV were conducted by modeling the cooling dynamics
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Model biological systems demonstrate the inducibility of pathways that strongly reduce cryoprotectant toxicity Cryobiology (IF 2.7) Pub Date : 2024-03-02 Anna Mazur, Srinivas Ayyadevara, Nirjal Mainali, Stephanie Patchett, Matthew Uden, Roberto I. Roa, Gregory M. Fahy, Robert J. Shmookler Reis
Cryoprotectant toxicity is a limiting factor for the cryopreservation of many living systems. We were moved to address this problem by the potential of organ vitrification to relieve the severe shortage of viable donor organs available for human transplantation. The M22 vitrification solution is presently the only solution that has enabled the vitrification and subsequent transplantation with survival
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The impact of heart valve and partial heart transplant models on the development of banking methods for tissues and organs: A concise review Cryobiology (IF 2.7) Pub Date : 2024-03-02 Andrew D. Vogel, Rebecca Suk, Christa Haran, Patrick G. Dickinson, Kristi L. Helke, Marc Hassid, David C. Fitzgerald, Joseph W. Turek, Kelvin G.M. Brockbank, Taufiek Konrad Rajab
Cryopreserved human heart valves fill a crucial role in the treatment for congenital cardiac anomalies, since the use of alternative mechanical and xenogeneic tissue valves have historically been limited in babies. Heart valve models have been used since 1998 to better understand the impact of cryopreservation variables on the heart valve tissue components with the ultimate goals of improving cryopreserved
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Development of sperm cryopreservation protocol for patin buah, Pangasius nasutus Cryobiology (IF 2.7) Pub Date : 2024-03-01 Nurizzati Idris, Muhammad Yazed Abduh, Noordiyana Mat Noordin, Ambok Bolong Abol-Munafi, Ivan Chong Chu Koh
The development of sperm cryopreservation for is necessary in order to serve the growing demand of this species through artificial fertilization and the preservation of valuable strains of male broodstocks. In the present study, the basic protocol of sperm cryopreservation for was established by identifying the optimal conditions for optimum cryoprotectant, toxicity of cryoprotectants, extenders, freezing
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Effects of apigenin and trans-ferulic acid on microscopic and oxidative stress parameters in the semen of water buffalo bulls during cryopreservation Cryobiology (IF 2.7) Pub Date : 2024-02-27 T. Mohammadi, M. hosseinchi Gharehaghaj, A. Alaei Novin
Cryopreservation involves exposing sperm to stressful conditions that affect cell viability. The high quality of the Azerbaijani water buffalo's by-products, such as buffalo milk, makes it a species of significant importance. Our focus is on protecting its genetic resources by preserving its sperm, as their numbers will decrease in the coming years and they are at risk of extinction. This study's goal
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Erythropoietin effects on cryopreserved/transplanted cat ovarian tissue: A comparison of two incubation methods Cryobiology (IF 2.7) Pub Date : 2024-02-27 Isabella M.G. Silva, Aline Q. Rodrigues, Rayane B. Ribeiro, Beatriz A. Aguiar, Anne E.S.P. Marinho, Elisa A.M. Souza, Yasmin B. Ferreira, Victoria C.O. Azevedo, Daniela M. Oliveira, Sônia N. Báo, Jair T. Goulart, Carolina M. Lucci, Fernanda Paulini
Many feline species are currently threatened with extinction. Therefore, germplasm bank establishment has become imperative. However, cryoinjury and ischemia-reperfusion injury pose significant obstacles to both cryopreservation and xenotransplantation. In this regard, erythropoietin (Epo) represents a potential alternative strategy due to its properties. This study aimed to assess the incubation of
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Cryopreservation of sperm from the gudgeon, Microphysogobio rapidus (Cyprinidae): Effects of cryoprotectant, diluents, and dilution ratio Cryobiology (IF 2.7) Pub Date : 2024-02-25 Irfan Zidni, Kang-Won Kim, Hyun-Seok Jang, Moon-Seong Heo, Keun-Sik Kim, Ju-Duk Yoon, Han-Kyu Lim
We investigated methods for cryopreserving sperm from the endangered gudgeon, , by examining the effects of cryoprotective agent (CPA) concentration, diluent, and dilution ratio on post-thaw sperm quality. The quality of frozen sperm was evaluated in terms of motility and kinematic parameters, viability, DNA damage, and fertilization rate. We evaluated methanol, glycerol, dimethyl sulfoxide (DMSO)
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Cryopreservation of human semen by inherently-controlled icing probability: Or how the surface profile of superhydrophobic carbon soot coatings and the sperm volume affect the outcome of slow freezing? Cryobiology (IF 2.7) Pub Date : 2024-02-22 Karekin D. Esmeryan, Todor A. Chaushev
The restoration of initial functionality of human spermatozoa subjected to cryopreservation is challenging, because the deleterious intracellular icing and the occurrence of osmotic shocks due to prolonged exposure to increased concentrations of intracellular solutes are oppositely dependent on the cooling rate. This longstanding problem could be overcome if using superhydrophobic soot coatings delaying
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Is cryoablation still suitable for advanced non-small cell lung cancer after failure of first-line chemotherapy? A multicenter, prospective, randomized-controlled trial of eighty-seven patients Cryobiology (IF 2.7) Pub Date : 2024-02-20 Yu Chen, Xueni Fang, Dan Wang, Quanwang Li, Kerui Zhang, Yuan Li, Jinghua Li, Haoyue Pang, Zhiqiang Cheng, Chunyang Zhang, Caiyun Zhang, Wuwei Yang, Baorang Zhu, Huanfang Fan, Changhui Han, Yonghui An, Lingling Zhang, Baoping Luo, Siqi Zhang, Taiying Lu, Yuanyuan Meng, Qinshu Jiao, Houlin Tang, Tian Zhou, Kaiwen Hu
The aim of this study was to investigate the therapeutic effect of cryoablation treatment in advanced NSCLC patients who had failed first-line chemotherapy. Eighty-seven patients from ten hospitals in China were enrolled into the study, forty-four patients received cryoablation treatment plus basic treatment (experimental group), and forty-three patients had basic treatment alone (control group). Follow-up
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Exploring the relationship between pollen viability and inclusion in Paeonia lactiflora after cryopreservation Cryobiology (IF 2.7) Pub Date : 2024-02-20 Mengting Zhu, Ying Peng, Jianzhou Gao, Ruifen Ren, Yingling Wan, Yan Liu
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Cryopreservation of the collector urchin embryo, Tripneustes gratilla Cryobiology (IF 2.7) Pub Date : 2024-02-16 Charley E. Westbrook, Jonathan Daly, Brian W. Bowen, Mary Hagedorn
The collector urchin, , is an ecologically important member of the grazing community of Hawai'i's coral reefs. Beyond its ability to maintain balance between native seaweeds and corals, has also been used as a food source and a biocontrol agent against alien invasive algae species. Due to overexploitation, habitat degradation, and other stressors, their populations face local extirpation. However,
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Effect of a magnetic field on droplet freezing and frost formation on cold surfaces Cryobiology (IF 2.7) Pub Date : 2024-02-15 Yujun Gou, Yi Qin, Jiachun Li, Wenbo Zhang, Jiacheng Liu, Zhe Wang
The effect of a magnetic field on condensed droplet freezing and frost formation was investigated using visualized experimental devices in this study. The size, shape, freezing time of droplets, and frost shape on the magnetic field considerably differ from those on the nonmagnetic surface. Moreover, the magnetic field could suppress droplet freezing and frost formation. The magnetic field suppression
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DNA fragmentation, antioxidant activity and histological structure of cryopreserved testicular tissue depending on sexual maturity and immunological status Cryobiology (IF 2.7) Pub Date : 2024-02-13 Nataliia Volkova, Mariia Yukhta, Anatoliy Goltsev
The objective of this work was to determine a relationship between a frequency of DNA fragmentation, a level of antioxidant activity and a preservation of histological structure depending on initial status of fragments of seminiferous tubules of testes (FSTT) of rats at the stages of cryopreservation. FSTT of animals of different ages (immature, mature), as well as animals with changed immunological
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Cryopreservation-induced delayed injury and cell-type-specific responses during the cryopreservation of endothelial cell monolayers Cryobiology (IF 2.7) Pub Date : 2024-02-11 MingHan Yu, Leah A. Marquez-Curtis, Janet A.W. Elliott
The cryopreservation of endothelial cell monolayers is an important step that bridges the cryopreservation of cells in suspension to that of tissues. Previous studies have identified clear distinctions in freezing mechanisms between cells in suspension and in monolayers, as well as developed novel protocols for monolayer cryopreservation. Recently, our group has shown that human umbilical vein endothelial
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Effects of taurine, cysteine and melatonin as antioxidant supplements to the freezing medium of Prochilodus brevis sperm Cryobiology (IF 2.7) Pub Date : 2024-02-10 Thais Maia Torres, Priscila Silva de Almeida-Monteiro, Renata Vieira do Nascimento, Silvio Alencar Cândido-Sobrinho, Carla Tatiana Nascimento Sousa, Yasmim Maia Ferreira, Kamila Teixeira de Paula, Carminda Sandra Brito Salmito-Vanderley
Cryopreservation consist of a set of methods to preserve cells and tissues by drastically reducing the temperature. Among some undesired effects, cryopreservation might generate reactive oxygen species that lead to an increase of oxidative stress, causing damage to cells. This study aimed to test taurine, cysteine, and melatonin on the freezing of sperm and assess its effects on post-thawed sperm quality
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The effect of the mitochondria-targeted antioxidant Mito-tempo during sperm ultra-rapid freezing Cryobiology (IF 2.7) Pub Date : 2024-02-08 Chun-Yuan Li, Juan Liu, Qi-Yuan Zheng, Nian Liu, Xi-Ling Huang, Yu-Yin Wu, Xing-Feng Yao, Qing-Ying Tan, Ying Huang, Chuan-Huo Hu, Chang-Long Xu
During the freeze-thaw process, human spermatozoa are susceptible to oxidative stress, which may cause cryodamage and reduce sperm quality. As a novel mitochondria-targeted antioxidant, Mito-tempo has been used for sperm cryopreservation. However, it is currently unknown what role it will play in the process of sperm ultra-rapid freezing. The purpose of this study was to investigate whether Mito-tempo
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Mesenchymal stromal cells derived from various tissues: Biological, clinical and cryopreservation aspects: Update from 2015 review Cryobiology (IF 2.7) Pub Date : 2024-02-08 Leah A. Marquez-Curtis, Janet A.W. Elliott
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Cryopreservation and recovery of a complex hypersaline microbial mat community Cryobiology (IF 2.7) Pub Date : 2024-02-07 Henk Bolhuis, Michele Grego
Cryopreservation of microorganisms is an essential tool in industrial- and food applications where conservation of microbial activity and critical beneficial traits need to be guaranteed to provide a consistent product or production process. This often refers to simple, single species or low diversity assemblages in liquid cultures that can easily be revived and regrown to perform the desired process
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Preserving frozen stallion sperm on dry ice using polymers that modulate ice crystalization kinetics Cryobiology (IF 2.7) Pub Date : 2024-02-01 Laura Uhlmannsiek, Hang Shen, Heinke Eylers, Gunilla Martinsson, Harald Sieme, Willem F. Wolkers, Harriëtte Oldenhof
Cryopreserved semen is routinely shipped in liquid nitrogen. Dry ice could serve as an alternative coolant, however, frozen storage above liquid nitrogen temperatures (LN2, −196 °C) may negatively affect shelf-life and cryosurvival. In this study, we determined critical temperatures for storage of cryopreserved stallion sperm. We evaluated: (i) effects of cooling samples to different subzero temperatures
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Polyvinyl alcohol addition to freezing extender can improve the post-thaw quality, longevity and in vitro fertility of ram epididymal spermatozoa Cryobiology (IF 2.7) Pub Date : 2024-02-01 Zeinab Hadi, Ebrahim Ahmadi, Naser Shams-Esfandabadi, Najmeh Davoodian, Abolfazl Shirazi, Midya Moradian
Recovering and cryopreserving epididymal spermatozoa are suitable methods for preserving the genetic potential of livestock and endangered species. Regarding encouraging reports on the use of polyvinyl alcohol (PVA) in cryopreserving various cell types, we conducted this study to examine the impact of PVA on the post-thaw quality, longevity, and in vitro fertility of ram epididymal sperm. In the first
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Enhancing cryopreservation survival in Petunia × Calibrachoa ‘Light Yellow’ callus: Insights into material characteristics and genetic integrity Cryobiology (IF 2.7) Pub Date : 2024-01-29 Yuanshan Zhang, Xiangxin Guo, Junyan Song, Guanqun Chen, Xiaohui Shen
Petunia × Calibrachoa ‘Light Yellow’ (× Petchoa ‘Light Yellow’) is a kind of perennial herbaceous flower obtained through intergeneric hybridization of Petunia and Calibrachoa with high ornamental value and wide application, facing challenges in seed acquisition. Expanding propagation through tissue culture is an economically efficient means. Hence, establishing an effective procedure for the storage
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Fatty acid production and associated gene pathways are altered by increased salinity and dimethyl sulfoxide treatments during cryopreservation of Symbiodinium pilosum (Symbiodiniaceae) Cryobiology (IF 2.7) Pub Date : 2024-01-30 Joseph K. Kihika, John K. Pearman, Susanna A. Wood, Lesley L. Rhodes, Kirsty F. Smith, Matthew R. Miller, Juliette Butler, Ken G. Ryan
The Symbiodinium genus is ancestral among other Symbiodiniaceae lineages with species that are both symbiotic and free living. Changes in marine ecosystems threaten their existence and crucial ecological roles. Cryopreservation offers an avenue for their long-term storage for future habitat restoration after coral bleaching. In our previous study we demonstrated that high salinity treatments of Symbiodiniaceae
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Cysteine supplementation pre-freeze and post-thaw improves integrity and reduces oxidative stress in cryopreserved ram spermatozoa Cryobiology (IF 2.7) Pub Date : 2024-01-28 Ahmed Kafi, Muhammad Khalid, Tharangani Herath, Claire Kershaw
Cryopreserved ram sperm is highly sensitive to oxidative stress by reactive oxygen species (ROS) which impair sperm function and integrity. Antioxidants such as cysteine can mitigate the effect of ROS, although the optimal concentration or timing of supplementation is unknown. This study aimed to determine the effect of concentration and timing of cysteine supplementation on the integrity and function
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Effect of type and concentration of antioxidant on sperm motility, viability, and DNA integrity of climbing perch Anabas testudineus Bloch, 1792 (Pisces: Anabantidae) post-cryopreservation Cryobiology (IF 2.7) Pub Date : 2024-01-17 Siti Maulida, Kartini Eriani, Nur Fadli, Mohd N. Siti-Azizah, Filiz K. Kocabas, Mehmet Kocabas, Martin Wilkes, Luvi S. Handayani, Sri R. Rahayu, Zainal A. Muchlisin
Sperm quality is preserved through the crucial involvement of antioxidants, which play a vital role in minimizing the occurrence of reactive oxygen species (ROS) during the cryopreservation process. The suitability of the type and concentration of antioxidants are species-dependent, and this study is crucial in order to improve the quality of the climbing perch sperm post-cryopreservation. Therefore
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Cryopreservation with DMSO affects the DNA integrity, apoptosis, cell cycle and function of human bone mesenchymal stem cells Cryobiology (IF 2.7) Pub Date : 2024-01-19 Yanqin Ding, Shuo Liu, Jianting Liu, Shenglin Jin, Jianjun Wang
Cryopreservation (CP) enables pooling and long-term banking of various types of cells, which is indispensable for the cell therapeutics. Dimethyl sulfoxide (DMSO) is universally used as a cryoprotectant in basic and clinical research. Although, the use of DMSO has been under serious debate due to significant clinical side effects correlated with infusions of cellular therapy products containing DMSO
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Effects of alpha lipoic acid supplementation on post-thaw quality of drone semen Cryobiology (IF 2.7) Pub Date : 2024-01-17 Ahmet Aktar, Selvinar Cakmak, Mehmed Berk Toker, İbrahim Cakmak, Kemal Bağci, Ümit Can Uzun, Selim Alcay
This study aimed to determine the effect of alpha-lipoic acid (ALA) on post-thaw quality of bee semen. In the study, semen from sexually mature drone were collected. A series of experiments were carried out in which the retrieved semen was diluted with diluents containing different ALA concentrations or without ALA supplement (control). Cryopreserved sperm were thawed, and evaluated for motility (phase-contrast
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Irreversible lipid phase transition detected in a porcine oocyte at chilling Cryobiology (IF 2.7) Pub Date : 2024-01-17 K.A. Okotrub, A.N. Omelchenko, E.A. Chuyko, S.Y. Amstislavsky, N.V. Surovtsev
Under physiological conditions, the membranes and lipid droplets of germ cells are in a conformationally disordered phase. Typically, during cooling, lipids undergo the transition to ordered phases and, upon heating, melt into a disordered phase. In this communication, we report the lipid phase transition in lipid droplets observed in porcine oocytes. Upon cooling, a sharp lipid phase transition from
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Effect of cryopreservation on motility, DNA integrity and gene expression in grey mullet, Mugil cephalus sperm Cryobiology (IF 2.7) Pub Date : 2024-01-12 Ramachandran Balamurugan, Sundaram Karthik, Venkatesan Arul
This study documents the effect of cryopreservation on motility, DNA integrity, and gene expression in Mugil cephalus sperm. Fresh sperm were cryopreserved using V2 extender (V2E) or 0.3 M glucose, each in combination with one of three cryoprotective agents (CPAs), i.e., 10 % of dimethylsulfoxide, ethylene glycol, or glycerol, all at once. After two different storage (7- vs 60- day) periods in liquid
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Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation Cryobiology (IF 2.7) Pub Date : 2024-01-04 Sevastiani Antonouli, Valentina Di Nisio, Christina Messini, Maria Samara, Andres Salumets, Alexandros Daponte, George Anifandis
Intracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization
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A novel in vitro model of clinical cryoablation to investigate the transition zone for focal tumor ablation Cryobiology (IF 2.7) Pub Date : 2024-01-01 Maura R. Vrabel, Christopher C. Fesmire, Matthew J. Rich, Robert L. Kobrin, Michael B. Sano, David A. Zaharoff
Cryoablation (CA) of solid tumors is highly effective at reducing tumor burden and eliminating small, early stage tumors. However, complete ablation is difficult to achieve and cancer recurrence is a significant barrier to treatment of larger tumors compared to resection. In this study, we explored the relationship between temperature, ice growth, and cell death using a novel in vitro model of clinical
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EtOH-LN cryoembedding workflow to minimize freezing artifact in frozen tissues: A pilot study in preparing tissues compatible with mass spectrometry-based spatial proteomics application Cryobiology (IF 2.7) Pub Date : 2023-12-27 Yong Chiang Tan, Muaatamarulain Mustangin, Nurwahyuna Rosli, Wan Syahira Ellani Wan Ahmad Kammal, Nurismah Md Isa, Teck Yew Low, Pey Yee Lee, Dinesh Kumar Chellappan, Razman Jarmin, Zamri Zuhdi, Azlanudin Azman, Chik Ian, Nursuhadah Mohamed Yusof, Lay Cheng Lim
Coolant-assisted liquid nitrogen (LN) flash freezing of frozen tissues has been widely adopted to preserve tissue morphology for histopathological annotations in mass spectrometry-based spatial proteomics techniques. However, existing coolants pose health risks upon inhalation and are expensive. To overcome this challenge, we present our pilot study by introducing the EtOH-LN workflow, which demonstrates
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CPA toxicity screening of cryoprotective solutions in rat hearts Cryobiology (IF 2.7) Pub Date : 2023-12-27 Casey J. Kraft, Bat-Erdene Namsrai, Diane Tobolt, Michael L. Etheridge, Erik B. Finger, John C. Bischof
In clinical practice, donor hearts are transported on ice prior to transplant and discarded if cold ischemia time exceeds ∼5 h. Methods to extend these preservation times are critically needed, and ideally, this storage time would extend indefinitely, enabling improved donor-to-patient matching, organ utilization, and immune tolerance induction protocols. Previously, we demonstrated successful vitrification
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Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation Cryobiology (IF 2.7) Pub Date : 2023-12-16 Mojtaba Baharsaadi, Maryam Hezavehei, Abdolhossein Shahverdi, Iman Halvaei
Human sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing
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Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility Cryobiology (IF 2.7) Pub Date : 2023-12-16 Shiyan Tang, Celine Jones, Jill Davies, Sheila Lane, Rod T Mitchell, Kevin Coward
The cryopreservation of immature testicular tissue (ITT) prior to gonadotoxic therapy is crucial for fertility preservation in prepubertal boys with cancer. However, the optimal holding time between tissue collection and cryopreservation has yet to be elucidated. Using the bovine model, we investigated four holding times (1, 6, 24, and 48 h) for ITTs before cryopreservation. Biopsies from two-week-old
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Comparison of liquid nitrogen-free slow freezing protocols toward enabling a practical option for centralized cryobanking of ovarian tissue Cryobiology (IF 2.7) Pub Date : 2023-12-12 Ebrahim Asadi, Atefeh Najafi, James D. Benson
Geographically distributed ovarian tissue cryobanks remain limited due to the high facility and staff costs, and cold transportation to centers is associated with ischemia-induced tissue damage that increases with transport distance. It is ideal to perform the cryopreservation procedure at a tissue removal site or local hospital before shipment to cost-effective centralized cryobanks. However, conventional
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Integrated metabolomics and proteomics analysis to understand muscle atrophy resistance in hibernating Spermophilus dauricus Cryobiology (IF 2.7) Pub Date : 2023-12-12 Kai Dang, Yuan Gao, Huiping Wang, Huajian Yang, Yong Kong, Shanfeng Jiang, Airong Qian
Hibernating Spermophilus dauricus experiences minor muscle atrophy, which is an attractive anti-disuse muscle atrophy model. Integrated metabolomics and proteomics analysis was performed on the hibernating S. dauricus during the pre-hibernation (PRE) stage, torpor (TOR) stage, interbout arousal (IBA) stage, and post-hibernation (POST) stage. Time course stage transition-based (TOR vs. PRE, IBA vs.
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Direct comparison of isobaric and isochoric vitrification of two aqueous solutions with photon counting X-ray computed tomography Cryobiology (IF 2.7) Pub Date : 2023-12-12 Jason T. Parker, Anthony N. Consiglio, Boris Rubinsky, Simo A. Mäkiharju
Vitrification is a promising approach for ice-free cryopreservation of biological material, but progress is hindered by the limited set of experimental tools for studying processes in the interior of the vitrified matter. Isochoric cryopreservation chambers are often metallic, and their opacity prevents direct visual observation. In this study, we introduce photon counting X-ray computed tomography
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Germplasm of Ozark chinquapin (Castanea ozarkensis Ashe) can be cryopreserved by dormant winter buds Cryobiology (IF 2.7) Pub Date : 2023-12-10 Maria M. Jenderek, Kathleen M. Yeater, Andrew L. Thomas
Ozark chinquapin (Castanea ozarkensis Ashe) is a forest tree, endemic to the Ozark Mountain region in Eastern United States. Its nutritious nuts were consumed by Native Americans, European settlers, livestock, and wild animals and its wood was an important rot-resistant construction material. Once a significant tree in regional forest communities, the species was nearly eradicated by a chestnut blight
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Vitrified turkey ovarian tissue cultured and assessed through gene expression patterns: A potential screening tool Cryobiology (IF 2.7) Pub Date : 2023-12-09 George B. Hall, Amy Shannon, Diane Hildenberger, Kristen Brady, Julie A. Long
Biobanking of turkey ovarian tissue has the potential to play a crucial part in preserving female genetics. To date, ovarian tissue has only been vitrified using a standard protocol, with immediate analyses after warming, therefore, long-term cryoinjury is unknown. Long-term cryoinjury was investigated here by in-ovo culturing, fresh (non-vitrified), a purposefully suboptimal poor vitrification (PV)
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Using a no-touch closed metal container for articular cartilage vitrification and storage Cryobiology (IF 2.7) Pub Date : 2023-12-07 Mohammadhamed Shahsavari, Janet A.W. Elliott, Nadr M. Jomha, Ana Paula Ribeiro Rodrigues
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Impact of mannitol on the integrity of ovarian tissue after extended hypothermic storage and cryopreservation Cryobiology (IF 2.7) Pub Date : 2023-12-07 Viktoriia Kiroshka, Iryna Rula
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Microencapsulation enabled high-hematocrit and glycerol-free cryopreservation of human RBCS Cryobiology (IF 2.7) Pub Date : 2023-12-07 Xianhui Qin, Gang Zhao
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Homoiothermal and heterothermal mammalian erythrocytes respond to artificial and natural hypobiosis with changes in relative content of main hemoglobin forms Cryobiology (IF 2.7) Pub Date : 2023-12-07 Svetlana Repina, Oleg Nardid
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Pretreatment strategies for the long-term conservation of tea (Camellia sinensis (L.) O. KUNTZE) GERMPLASM Cryobiology (IF 2.7) Pub Date : 2023-12-07 Shailika Sharma, Papiya Mukherjee
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Magnetic nanoparticles decreased ROS levels in vitrified-warmed mouse immature and mature oocytes Cryobiology (IF 2.7) Pub Date : 2023-12-07 Pegah Shayegh, Farzaneh Baniasadi, Hasan Rajabimaham, Mohammad Reza Ghalamboran, Rouhollah Fathi
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Erythrocyte permeability to cryoprotectants via novel model-based analysis of osmotic shock Cryobiology (IF 2.7) Pub Date : 2023-12-07 Olha Denysova, Larisa A. Vodopyanova, Johannes M. Nitsche
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Phase state of bay leaves (Laurus nobilis) LIPID FRACTION AT LOW TEMPERATURES Cryobiology (IF 2.7) Pub Date : 2023-12-07 Olena Bobrova, Stanislav Narozhnyi, Stanislav Sevastianov, Aleksandr Osetsky, Anton Prystalov, Oleg Nardid
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Cryopreservation of rat fetal neural cells as a component of suspension and aggregates Cryobiology (IF 2.7) Pub Date : 2023-12-07 Oleksandr Sukach, Olga Ochenashko, Svitlana Vsevolodska
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Experimental study of the effect of alternating electric fields on the freeze-thaw process of solutions Cryobiology (IF 2.7) Pub Date : 2023-12-07 Zirui Wang, Qi An, Gang Zhao
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Effect of hypothermic storage upon red blood cell morphology and osmotic stability Cryobiology (IF 2.7) Pub Date : 2023-12-07 Nikolay Repin, Larysa Marchenko, Tetiana Govorukha, Vera Strona
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Does cryopreservation of human oocytes and embryos affect ability to hatch? Cryobiology (IF 2.7) Pub Date : 2023-12-07 N.S. Shapovalova, T.O. Yurchuk, V.I. Pinyaev, M.P. Petrushko
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Recent advances in modeling works for cell cryopreservation Cryobiology (IF 2.7) Pub Date : 2023-12-07 Kashan Memon, Gang Zhao
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Impact of cryopreservation on human sperm quality: a comparative study between semen and processed sperm cryopreservation Cryobiology (IF 2.7) Pub Date : 2023-12-07 Huidrom Yaiphaba Meitei, Shubhashree Uppangala, Guruprasad Kalthur, Stefan Schlatt, Satish Kumar Adiga
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Vitrification effect on mitochondrial function of human embryos Cryobiology (IF 2.7) Pub Date : 2023-12-07 Hanieh Bayat, Mohammad Akbari, Samaneh Adib, Maryam Jahangiri, Elham Abedheydari, Maryam Mohammadi, Bahar Movaghar
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Investigating the antifreeze effect of FFIBP protein faces on primary prismatic plane of ice through molecular dynamics simulation Cryobiology (IF 2.7) Pub Date : 2023-12-07 Hamed Zahraee, Mohammad Reza Housaindokht, Fatemeh Mohammadi, Mansour Mashreghi
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Interfacial adsorption mechanism of FFIBP Ice-binding protein with gold nanoparticle surface (111): insights from atomistic simulations Cryobiology (IF 2.7) Pub Date : 2023-12-07 Hamed Zahraee, Mohammad Reza Housaindokht, Fatemeh Mohammadi, Mansour Mashreghi