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A single administration of carbetocin before electroejaculation increases the insemination doses produced from each ejaculate in rams Theriogenology (IF 2.8) Pub Date : 2024-03-15 J.C. Orihuela, A. Freitas-de-Melo, L. Pinto-Santini, J. Giriboni, M.N. Viera, J. Santiago-Moreno, F. Beracochea, P. Silveira, R. Ungerfeld
The aim of the study was to determine the effect of carbetocin administration (a long-acting analog of oxytocin) 20 or 10 min before electroejaculation (EE) on the duration of semen collection procedure, quantitative and qualitative characteristics of the ejaculate, and stress biomarkers in rams. Semen was collected from 12 Corriedale rams (age, 2.5–5.5 years old) with EE, in a Latin-square design
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Enhancement of progesterone biosynthesis via kisspeptin stimulation: Upregulation of steroidogenic transcripts and phosphorylated extracellular signal-regulated kinase (p-ERK1/2) expression in buffalo luteal cells Theriogenology (IF 2.8) Pub Date : 2024-03-14 M.P. Thejaswini, M.K. Patra, R. Sharma, Md R.A. Raza, J.K. Sasidharan, M. Karikalan, Z.B. Dubal, S.K. Ghosh, G.K. Gaur, S.K. Singh, N. Krishnaswamy
The presence of Kisspeptin (Kp) and its receptors in the corpus luteum (CL) of buffalo has recently been demonstrated. In this study, we investigated the role of Kp in the modulation of progesterone (P) synthesis . The primary culture of bubaline luteal cells (LCs) was treated with 10, 50, and 100 nM of Kp and Kp antagonist (KpA) alongside a vehicle control. The combined effect of Kp and KpA was assessed
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Heme oxygenase 1 (HO1) regulates autophagy and apoptosis via the PI3K/AKT/mtor signaling pathway of yak sertoli cells Theriogenology (IF 2.8) Pub Date : 2024-03-13 Qiu Yan, Qi Wang, Jinghong Nan, Tingting Chen, Juntao Wang, Yong Zhang, Ligang Yuan
Successful male reproduction depends on healthy testes. Autophagy has been confirmed to be active during many cellular events associated with the testes. It is not only crucial for testicular spermatogenesis but is also an essential regulatory mechanism for Sertoli cell (SCs) ectoplasmic specialization integrity and normal function of the blood-testis-barrier. Hypoxic stress induces oxidative damage
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Role and action mechanisms of miR-149 and miR-31 in regulating function of pig cumulus cells and oocytes Theriogenology (IF 2.8) Pub Date : 2024-03-11 Jia-Shun Wu, Shuai Gong, Min Zhang, Rui-Jie Ma, Hui-Li Wang, Ming-Jiu Luo, Nan He, Jing-He Tan
Understanding the mechanisms for oocyte maturation and optimizing the protocols for in vitro maturation (IVM) are greatly important for improving developmental potential of IVM oocytes. The miRNAs expressed in cumulus cells (CCs) play important roles in oocyte maturation and may be used as markers for selection of competent oocytes/embryos. Although a recent study from our group identified several
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Optimizing ReBreed21 I: Evaluation of endocrine and ovarian dynamics in non-bred Bos indicus heifers Theriogenology (IF 2.8) Pub Date : 2024-03-08 João Paulo N. Andrade, Rafael R. Domingues, Bruno P. Carvalho, Victor Gomez-Leon, Alexandre B. Prata, Roberto Sartori, Milo C. Wiltbank
The present study evaluated follicular and endocrine dynamics during ReBreed21, a reproductive strategy that allows resynchronization of ovulation every 21 days in (Nelore) heifers. A synchronized estrous cycle was induced using a standard timed ovulation protocol (d −10: P4 implant inserted + 2 mg estradiol benzoate; d −2: P4 removed+ 0.5 mg cloprostenol + 0.6 mg estradiol cypionate + 200 IU equine
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Estrogens improve the pregnancy rate in cattle: A review and meta-analysis Theriogenology (IF 2.8) Pub Date : 2024-03-07 Ebrahim Taghizadeh, Farid Barati, Aziz A. Fallah, Maryam Hemmatzadeh-Dastgerdi, Mohammad-Saleh Nejabati
Estrogens have proven to be effective in bovine estrus induction protocols. Considering the extensive use of these products in large-scale estrus synchronization, the primary objective of the present study was to assess their effects on pregnancy rate (PR) using a meta-analysis approach. A total of 797 papers were screened from three major databases (PubMed, Web of Science, Scopus). Sixty-one studies
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Characterization of metabolic patterns in porcine cumulus cells during meiotic maturation Theriogenology (IF 2.8) Pub Date : 2024-03-07 Ming Gao, Hengjie Wang, Minjian Chen, Shuai Zhu, Yongfu He, Qiang Wang, Ling Gu
Metabolic coupling between oocytes and the surrounding somatic cells allows for normal two-way communication, and their interactions is necessary for generating developmentally competent eggs. However, the metabolic framework that support oocyte maturation in surrounding cumulus cells is still lacking. Herin, we established a temporal metabolome profile of porcine cumulus cells at three key stages
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Schisandrin B enhances embryo competence and potentially mitigates endoplasmic reticulum stress during porcine preimplantation development Theriogenology (IF 2.8) Pub Date : 2024-03-05 Chuang Li, Kuk Bin Ji, Ho Yong Choi, Haixing Liu, Minkyu Kim
Endoplasmic reticulum (ER) stress induced by agents such as tunicamycin (TM) substantially impedes the developmental progression of porcine embryos. Lignan compounds such as Schisandrin B (Sch-B), may have the potential to mitigate this stress. However, there are few studies on the effects of Sch-B on embryo development. To address this research gap, this study evaluates the protective efficacy of
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Developmental programming of production and reproduction in dairy cows: IV. Association of maternal milk fat and protein percentage and milk fat to protein ratio with offspring's birth weight, survival, productive and reproductive performance and AMH concentration from birth to the first lactation period Theriogenology (IF 2.8) Pub Date : 2024-03-02 Emadeddin Mobedi, Hamid Reza Dehghan Harati, Iman Allahyari, Faramarz Gharagozlou, Mehdi Vojgani, Reza Hemmati Baghbanani, Amirsina Akbarinejad, Vahid Akbarinejad
Although the association of maternal milk production with developmental programming of offspring has been investigated, there is limited information available on the relationship of maternal milk components with productive and reproductive performance of the offspring. Therefore, the present study was conducted to analyze the association of maternal milk fat and protein percentage and milk fat to protein
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Expression of IZUMO1 and JUNO in the gonads of domestic cats (Felis catus) Theriogenology (IF 2.8) Pub Date : 2024-03-01 Phakjira Sanguansook, Watchareewan Rodprasert, Chenphop Sawangmake, Lourdes Gimeno, José Luis Ferran, Pilar Sòria-Monzó, Manuel Avilés, Mª José Izquierdo Rico, Kaywalee Chatdarong
Because of the time-consuming nature of surgical neutering and the rapid rate of reproduction among domestic cats, it is crucial to investigate alternative, nonsurgical methods of contraception for this species. Sperm protein IZUMO1 and its oocyte receptor JUNO have been proposed as potential targets for nonsurgical contraceptives. This study aimed to demonstrate (1) the protein coding sequence of
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Neddylation inhibition affects early embryonic development by disrupting maternal-to-zygotic transition and mitochondrial function in mice Theriogenology (IF 2.8) Pub Date : 2024-02-28 Mingxiao Liu, Zhiming Ding, Peihao Sun, Shuo Zhou, Hanxiao Wu, Lijun Huo, Liguo Yang, John S. Davis, Aixin Liang
Post-translational modifications (PTMs) are critical for early development in mice because early cleavage-stage embryos are characterized by transcriptional inactivity. Neddylation is an important ubiquitin-like PTM that regulates multiple biophysical processes. However, the exact roles of neddylation in regulating early embryonic development remain largely unknown. In the present study, we found that
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Target silencing of porcine SPAG6 and PPP1CC by shRNA attenuated sperm motility Theriogenology (IF 2.8) Pub Date : 2024-02-28 Hongyan Ren, Yandi Zhang, Yanzhen Bi, Heng Wang, Guijie Fang, Pengxiang Zhao
The quality of sperm significantly influences the reproductive efficiency of pig herds. High-quality sperm is necessary for efficient fertilization and to maximize the litter numbers in commercial pig farming. However, the understanding of genes regulating porcine sperm motility and viability is limited. In this study, we validated porcine sperm/Sertoli-specific promoters through the luciferase reporter
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Comparison of ICSI, IVF, and in vivo derived embryos to produce CRISPR-Cas9 gene-edited pigs for xenotransplantation Theriogenology (IF 2.8) Pub Date : 2024-02-27 Olinda Briski, Gastón Emilio La Motta, Laura Daniela Ratner, Federico Andrés Allegroni, Santiago Pillado, Guadalupe Álvarez, Betiana Gutierrez, Lisa Tarragona, Andrea Zaccagnini, Marcelo Acerbo, Carla Ciampi, Rafael Fernández-Martin, Daniel Felipe Salamone
Genome editing in pigs for xenotransplantation has seen significant advances in recent years. This study compared three methodologies to generate gene-edited embryos, including co-injection of sperm together with the CRISPR-Cas9 system into oocytes, named ICSI-MGE (mediated gene editing); microinjection of CRISPR-Cas9 components into oocytes followed by fertilization (IVF), and microinjection of fertilized
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Sperm binding to oviduct epithelial spheroids varies among males and ejaculates but not among females in pigs Theriogenology (IF 2.8) Pub Date : 2024-02-27 Lorraine Schmaltz, Théo Prudhomme, Guillaume Tsikis, Karine Reynaud, Isabelle Mérour, Pascal Mermillod, Marie Saint-Dizier
The elimination of ejaculates and males with low fertility despite good sperm motility and morphology is crucial to maintain high pregnancy rates after artificial insemination (AI) in farm animals. The ability of sperm to survive in the female tract is particularly crucial in pigs due to the large variation in the timing between AI and ovulation and the high number of oocytes to fertilise. The objective
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Characterizing the relationship between gonadotropin releasing hormone (GnRH), kisspeptin, and RFamide related peptide 3 (RFRP-3) neurons in the equine hypothalamus across the estrous cycle and in the anovulatory seasons Theriogenology (IF 2.8) Pub Date : 2024-02-27 B.M. McGrath, S.T. Norman, C.A. Gaspardis, J.L. Rose, C.J. Scott
To understand better the role that kisspeptin plays in regulating seasonal and estrous cycle changes in the mare, this study investigated the number, location and interactions between GnRH, kisspeptin and RFRP-3 neurons in the equine hypothalamus. Hypothalami were collected from mares during the non-breeding season, vernal transition and various stages of the breeding season. Fluorescent immunohistochemistry
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Seminal extracellular vesicles alter porcine in vitro fertilization outcome by modulating sperm metabolism Theriogenology (IF 2.8) Pub Date : 2024-02-26 Isabel Barranco, Marcella Spinaci, Salvatore Nesci, Yentel Mateo-Otero, Vito Antonio Baldassarro, Cristina Algieri, Diego Bucci, Jordi Roca
Porcine seminal plasma (SP) is loaded with a heterogeneous population of extracellular vesicles (sEVs) that modulate several reproductive-related processes. This study investigated the effect of two sEV subsets, small (S-sEVs) and large (L-sEVs), on porcine fertilization (IVF). The sEVs were isolated from nine SP pools (five ejaculates/pool) using a size-exclusion chromatography-based procedure and
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Meta-analysis to determine efficacy of colony-stimulating factor 2 for improving pregnancy success after embryo transfer in cattle Theriogenology (IF 2.8) Pub Date : 2024-02-23 Peter J. Hansen, Eliab Estrada-Cortés, Thiago F. Amaral, Rosabel Ramírez-Hernández
Results have been inconsistent as to whether addition of colony stimulating factor 2 (CSF2) to culture medium improves embryo competence for establishment of pregnancy in cattle and humans. The purpose of the current study was to use all available experiments in cattle concerning effects of CSF2 on pregnancy success after transfer into recipient cattle. The approach was to perform a meta-analysis of
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Circular RNA circRPS19 promotes chicken granulosa cell proliferation and steroid hormone synthesis by interrupting the miR-218-5p/INHBB axis Theriogenology (IF 2.8) Pub Date : 2024-02-23 Yuanhang Wei, Xiaoxu Shen, Xiyu Zhao, Haorong He, Yao Zhang, Qing Zhu, Huadong Yin
Ovarian follicle development is an important physiological activity for females and makes great significance in maintaining female health and reproduction performance. The development of ovarian follicle is mainly affected by the granulosa cells (GCs), whose growth is regulated by a variety of factors. Here, we identified a novel circular RNA (circRNA) derived from the Ribosomal protein S19 (RPS19)
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Growth differentiation factor 9 regulates the expression of estrogen receptors via Smad2/3 signaling in goat cumulus cells Theriogenology (IF 2.8) Pub Date : 2024-02-22 Yaju Tang, Sihai Lu, Juncai Wei, Rui Xu, Hui Zhang, Qiang Wei, Bin Han, Yan Gao, Xiaoe Zhao, Sha Peng, Menghao Pan, Baohua Ma
Both oocyte secretory factors (OSFs) and estrogen are essential for the development and function of mammalian ovarian follicles, playing synergistic role in regulating oocyte growth. OSFs can significantly affect the biological processes regulated by estrogen in cumulus cells (CCs). It is a scientific question worth investigating whether oocyte secretory factors can influence the expression of estrogen
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The lack of the influence of various species of Mycoplasma spp. on canine semen quality Theriogenology (IF 2.8) Pub Date : 2024-02-22 K. Domrazek, P. Konieczny, M. Majka, M. Czopowicz, A. Cywińska, P. Jurka
Mycoplasmas colonize fish, reptiles, birds and mammals, being commensals or causing diseases, sometimes severe in ruminants, swine, poultry, or wildlife animals. So far, 15 species of canine spp. have been described. Conflicting results have been presented regarding the pathogenicity of spp. Although many virulence factors of these bacteria have been described, they still require attention. The main
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Characterization of placentome vascular perfusion in relation to pregnancy associated glycoproteins throughout gestation in pregnant beef heifers Theriogenology (IF 2.8) Pub Date : 2024-02-22 C.K. Griffin, C.O. Lemley, K.G. Pohler, X. Sun, A.S. Lear
During pregnancy, blood flow to the uterus changes to support fetal demand. Placentomes serve as vascular attachment sites on the placenta for exchange of gases, nutrients, and metabolic products. Non-invasive methods of ultrasonography and biomarkers have been described to assess placental health and fetal viability. Pregnancy associated glycoproteins (PAGs) are produced by the ruminant placenta and
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Effect of estradiol after bacterial infection on the Wnt/β-catenin pathway in bovine endometrium epithelial cells and organoids Theriogenology (IF 2.8) Pub Date : 2024-02-22 Yalin Zhang, Yujie Zhuang, Jin Zhou, Xiaoyu Xie, Mingzhu Sun, Mengyao Zheng, Keyun Yuan, Zhiping Zhang, Juntao Zhang
Endometritis is a disease caused by a postpartum bacterial infection with a poor prognosis that primarily affects dairy cows. Three-dimensional organoids have been used as a model for endometritis, because they exhibit a structure comparable to that of the endometrium, demonstrating both expansibility and hormone responsiveness. These characteristics render them an ideal platform for in vitro investigations
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A repertoire of single nucleotide polymorphisms (SNPs) of major fecundity BMPR1B gene among 75 sheep breeds worldwide Theriogenology (IF 2.8) Pub Date : 2024-02-20 Chunna Cao, Qian Zhou, Yuxin Kang, Zhanerke Akhatayeva, Peiyao Liu, Yuheng Bai, Ran Li, Yu Jiang, Qingfeng Zhang, Xianyong Lan, Chuanying Pan
The gene is a major determinant of sheep reproductive capacity. Previous studies revealed that Q249R (FecB) is a profound variant of that influences the ovulation rate and litter size in sheep. However, unlike Q249R locus, the full spectrum of single nucleotide polymorphisms (SNPs) within has not been extensively studied. A systematic screen of SNPs in would facilitate the discovery of novel variants
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Change in the swimming pattern of Salmo salar spermatozoa caused by the high temperature of the sperm motility activation medium Theriogenology (IF 2.8) Pub Date : 2024-02-18 Osvaldo Merino, Elías Figueroa, Iván Valdebenito, Jennie Risopatrón, Maxsihel Merino, Jorge G. Farías
Fish are ectotherms and many have an external reproductive mode. An environmental factor which triggers fish reproductive activity in fish is water temperature. However, climate change is causing increasingly frequent events in which the water temperature varies rapidly; as a result, both in hatchery and in natural conditions, fish sperm are exposed to varying environmental temperatures during their
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Artificial insemination of all ejaculated sperm fractions accelerates embryo development and increases the uterine vascularity in the pig Theriogenology (IF 2.8) Pub Date : 2024-02-17 Santa María Toledo-Guardiola, Ester Párraga-Ros, Juan Seva, Chiara Luongo, Francisco A. García-Vázquez, C. Soriano-Úbeda, C. Matás
The semen of boar is characterized by ejaculation in well-differentiated fractions with specific concentration, composition, and volume. The ‘sperm-rich fraction' (SRF), the most concentrated seminal fraction, is habitually collected in insemination centers to make artificial insemination (AI) doses. The absence of the other fractions in AI doses could alter the uterine reaction to AI and not trigger
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Evaluation of blastocyst re-expansion, quality in relation to storage temperature, and sexing using blastocoel fluid after manual perforation with a hand-held needle involving in vivo produced equine embryos Theriogenology (IF 2.8) Pub Date : 2024-02-16 Giovana Siqueira Camargo, Luiz Daniel de Barros, José Paes Oliveira-Filho, Cristiana Raach Bromberger, Luciane Alarcao Dias-Melicio, Leandro Alves dos Santos, Don R. Bergfelt, Erica Rodrigues Ferraz de Andrade, Heloisa Siqueira Canesin, Cezinande de Meira, Fernanda Saules Ignácio
The present study was designed to evaluate equine blastocyst re-expansion rate, quality, and sex following perforation of the blastocoel, collection of blastocoel fluid (BF), and PCR amplification of free DNA. Experiment 1 tested the feasibility of the BF sample collection with a hand-held, small-gauged needle (26g) and subsequent PCR amplification of the TSP-Y gene for males and AMEL-Y gene for males
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Exosomes from porcine serum as endogenous additive maintain function of boar sperm during liquid preservation at 17 °C in vitro Theriogenology (IF 2.8) Pub Date : 2024-02-16 Yang Wang, Qimin Liu, Qingfang Sun, Lijuan Zheng, Tianqi Jin, Heran Cao, Chao Zhu, Long Li, Ye Gong, Fangxia Yang, Wuzi Dong
The supplementation of sperm culture media with serum is quite common, and improves both sperm survival and motility. However, the link between serum and sperm remains poorly understood. The present study is the first investigation of the effects on sperm quality and function of endogenous porcine serum exosomes in medium used for culturing boar sperm. Scanning electron microscopy (SEM) confirmed that
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The role of equine seminal plasma derived cysteine rich secretory protein 3 (CRISP3) in the interaction between polymorphonuclear neutrophils (PMNs) and populations of viable or dead spermatozoa, and bacteria Theriogenology (IF 2.8) Pub Date : 2024-02-15 Andria L. Doty, Lynda M.J. Miller, Carleigh E. Fedorka, Mats H.T. Troedsson
Breeding-induced endometritis is a physiological reaction to clear the uterus from excess spermatozoa and bacteria after breeding. Cysteine rich secretory protein 3 in seminal plasma (spCRISP3) protects spermatozoa from binding and destruction by uterine PMNs, but it is not clear if this involves all sperm and bacteria, or if it is selective to a sub-population of live sperm. The objective of this
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A mutation in LPAR2 activates the miR-939-5p-LPAR2-PI3K/AKT axis to regulate the proliferation and apoptosis of granulosa cells in sheep Theriogenology (IF 2.8) Pub Date : 2024-02-15 Runan Zhang, Yufang Liu, Wentao Li, Peng Wang, Ziyi Liu, Yuliang Wen, Mingxing Chu, Linjie Wang
Lysophosphatidic acid receptor-2 () is a G protein-coupled receptor, which is involved in various physiological processes such as cell development, proliferation, and apoptosis, and is thought to play an important role in follicular development and reproduction. There is evidence that miRNA recognition elements (MRE) in the gene 3′UTR often contain single nucleotide polymorphisms (SNPs) that can alter
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In vitro effects of different ozone preparations on microorganisms responsible for endometritis in the mare Theriogenology (IF 2.8) Pub Date : 2024-02-15 Gian Guido Donato, Patrizia Nebbia, Maria Cristina Stella, Daniela Scalas, Denis Necchi, Alessia Bertero, Giovanna Romano, Lara Bergamini, Mariagrazia Lucia Poletto, Andrea Peano, Patrizia Robino, Tiziana Nervo
Infectious endometritis is considered one of the major causes of infertility and it can affect up to 60% of barren mares. It is characterized by the presence of one or more microorganisms in the reproductive tract and it is treated with the administration of antibiotics, ecbolic agents and uterine lavages. Ozone, thanks to its antimicrobial properties that are based on its high oxidative potential
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Sperm quality variables of sex-sorted bull semen produced by magnetic-activated cell sorting coupled with recombinant antibodies targeting Y-chromosome-bearing sperm Theriogenology (IF 2.8) Pub Date : 2024-02-15 Phanuwit Paitoon, Apinya Sartsook, Marninphan Thongkham, Anucha Sathanawongs, Chompunut Lumsangkul, Wiwat Pattanawong, Surat Hongsibsong, Korawan Sringarm
The immunological sexing method using antibodies offers cost-effective, high-volume production but faces challenges in terms of X-sperm purity in sexed semen. This research aimed to produce sexed bull semen using highly specific recombinant antibodies in magnetic-activated cell sorting (MACS), evaluate sperm quality and kinematic parameters, and verify the sex ratio of sperm, embryos, and live calves
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Genetic variation in the BLM gene and its expression in the ovaries is closely related to kidding number in goats Theriogenology (IF 2.8) Pub Date : 2024-02-14 Dongwei An, Xiang Chen, Ziyang Li, Lingang Dai, Jiajin Huang, Meimei Xiao, Huan Liu, Jiali Xu, Yong Ruan
Bloom (BLM) helicase plays an important role in DNA replication and the maintenance of genome integrity. BLM protein deficiency, which plays a vital role in the sperm-egg union and germ-cell development during reproduction, can lead to severe DNA damage in goats. However, the effect of BLM protein deficiency on goat litter size has not been reported. Herein, we studied the association between the genetic
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MUC1 regulation in the left and right uterine horns and conceptus trophectoderm during the peri-implantation period of dromedary camel Theriogenology (IF 2.8) Pub Date : 2024-02-13 Mohammed Salem Moqbel, Saeed Yaseen Al-Ramadan
Pregnancy maintenance in dromedary camels poses significant challenges, including early embryonic loss in the left uterine horn (LH) and unsuccessful pregnancy in the right uterine horn (RH), suggesting a potential asynchrony between conceptus signaling and uterine receptivity. The transition of the uterine epithelium from a pre-receptive to a receptive state requires a delicate balance of adhesion-promoting
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Pharmacokinetics of florfenicol in serum and seminal plasma in beef bulls Theriogenology (IF 2.8) Pub Date : 2024-02-11 Juan E. Romano, Anisa Bardhi, Giampiero Pagliuca, Graciela B. Villadόniga, Andrea Barbarossa
The objectives of this study were to compare the serum and seminal plasma pharmacokinetic profiles of florfenicol (FLO) and florfenicol amine (FLA) after the administration of FLO either by IM or SC routes in beef bulls. Four clinically healthy Hereford bulls underwent a comprehensive physical exam, including breeding soundness examination, CBC, and chemistry profile panel. Bulls were healthy and classified
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Application of sperm motion kinematics and motility-related proteins for prediction of male fertility Theriogenology (IF 2.8) Pub Date : 2024-02-07 Jeong-Won Bae, Ju-Mi Hwang, Woo-Jin Lee, Dae-Hyun Kim, Jun Koo Yi, Jae Jung Ha, Dong Yep Oh, Woo-Sung Kwon
The selection of superior sires is paramount for enhancing the efficiency of animal production in the livestock industry. However, semen quality assessment still relies on conventional semen analysis techniques in both animals and humans. Despite extensive efforts to develop various biomarkers for more accurate and precise predictions of male fertility potential, more effective physiological indicators
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Effects of equine chorionic gonadotropin dosage and its splitting in different days on reproductive performance of Nellore cows synchronized for timed-artificial insemination Theriogenology (IF 2.8) Pub Date : 2024-02-06 A.F.F. Sales, B.I. Cappellozza, E. Vilela, I. Claro Júnior, O.G. Sá Filho, J.L.M. Vasconcelos
This study evaluated the effects of dose of equine chorionic gonadotropin () and its splitting in different days of the synchronization protocol on reproductive performance of primiparous and multiparous Nellore cows. In the present study, 2,536 Nellore cows (1,634 primiparous and 902 multiparous) were assigned to receive in a 2 × 2 factorial design 1) an intravaginal progesterone () device and 2.0
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The dependence between glycodelin and selected metalloproteinases concentrations in bovine placenta during early gestation and parturition with and without retained foetal membranes Theriogenology (IF 2.8) Pub Date : 2024-02-05 Jacek Wawrzykowski, Monika Jamioł, Marta Kankofer
Pregnancy course depends on the appropriate connection between the mother and the developing foetus. Pregnancy is completed when the placenta is timely expelled. Placental retention is one of the possible pregnancy complications. Extracellular matrix, including adhesive proteins and enzymes that can break down collagens, seems to be responsible for it. The aim of the present study was to examine the
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The presence of estradiol benzoate in the cervical relaxation treatment for non-surgical embryo collection does not impair embryonic morphological quality, cryosurvival, and gene expression profile Theriogenology (IF 2.8) Pub Date : 2024-02-04 Ribrio Ivan Tavares Pereira Batista, Jenniffer Hauschildt Dias, Joedson Dantas Gonçalves, Gabriel Brun Vergani, Maria Emilia Franco Oliveira, Joanna Maria Gonçalves Souza-Fabjan, Jeferson Ferreira Fonseca
Non-surgical embryo recovery (NSER) is usually preceded by a cervical relaxation in ovine donors, based on estradiol benzoate (EB), prostaglandin (PGF), and oxytocin (OT). However, it is hypothesized that, due to poorly understood mechanisms, EB can result in embryotoxic actions. To evaluate this, 20 min before NSER superovulated sheep were induced to cervical relaxation with 0.0 (G0.0), 0.5 (G0.5)
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SIRT2 regulates apoptosis by inducing mitophagy in sheep cumulus cells Theriogenology (IF 2.8) Pub Date : 2024-02-03 Xiaohuan Fang, Wei Xia, Yatian Qi, Yang Yu, Qingyi Sun, Di Zhang, Zhenmin Zhou, Tianmiao Qin, Chenyu Tao, Junjie Li
Cumulus cells surrounding oocytes furnish nutritional support crucial for oocyte maturation , and thereby enhance oocyte quality significantly. Our previous studies affirmed the role of SIRT2 in regulation of mitochondrial function in sheep granulosa cells. However, the effect of SIRT2 action on mitophagy in these cells remain unclear. Here, RNA-seq was used to scrutinize pathways where differentially
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Lactation body condition loss impaired conceptus development and plasma progesterone concentration at day 8 post-ovulation in primiparous sows Theriogenology (IF 2.8) Pub Date : 2024-02-03 Hao Ye, Nicoline M. Soede, Bas Kemp, Junjun Wang, Marleen Fleuren, Bjorge Laurenssen, Emmy Bouwman, Pieter Langendijk
The current study investigated effects of dietary amino acid (AA) availability on lactational body condition loss and metabolic status, in relation to reproductive parameters after weaning up to Day 8 post-ovulation. Primiparous sows (n = 35) were allocated to one of two lactation diets containing either low crude protein (CP, 140 g/kg) with a low percentage (8%) of slow protein in total protein (LL
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The ability of donkey sperm to induce oocyte activation and mule embryo development after ICSI Theriogenology (IF 2.8) Pub Date : 2024-02-02 Camila Arroyo-Salvo, Marïa Y. Cogollo Villarreal, Gabriel Clérico, Ana P. Flores Bragulat, Andrea Niño Vargas, Catalina Castañeira, Olinda Briski, Carolina Alonso, Jessica Plaza, Jose M. Zeledon, Luis Losinno, Marcelo Miragaya, Marina Sansinena, Silvina Perez Martinez, Andrés Gambini
Members of the genus exhibit a fascinating capacity for hybridization, giving rise to healthy offspring. Mules, resulting from the mating of a mare with a jack, represent the most prevalent equid hybrid, serving diverse roles in our society. While embryo production, particularly through Intracytoplasmic Sperm Injection (ICSI), has rapidly gained significance in domestic horses, the production in other
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Altered luteal expression patterns of genomic and non-genomic progesterone receptors in bitches at different reproductive states Theriogenology (IF 2.8) Pub Date : 2024-02-02 Eyyup Hakan Ucar, Cevdet Peker, Mustafa Hitit, Mehmet Kose, Musa Tatar, Faruk Bozkaya, Mehmet Osman Atli
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Transcriptome analysis of porcine embryos derived from oocytes vitrified at the germinal vesicle stage Theriogenology (IF 2.8) Pub Date : 2024-02-02 Baoyu Jia, Decai Xiang, Han Yang, Jiachong Liang, Chunrong Lv, Qige Yang, Xinyu Huang, Guobo Quan, Guoquan Wu
Vitrification of porcine immature oocytes at the germinal vesicle (GV) stage reduces subsequent embryo yield and changes at the molecular level may occur during embryonic development. Therefore, the present study used porcine parthenogenetic embryos as a model to investigate the effect of GV oocyte vitrification on the transcriptional profiles of the resultant embryos at the 4-cell and blastocyst stages
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Factors affecting the analysis and interpretation of sperm quality in frozen/thawed stallion semen Theriogenology (IF 2.8) Pub Date : 2024-01-29 Camilo Hernández-Avilés, Luisa Ramírez-Agámez, Dickson D. Varner, Charles C. Love
In the current study, we examined: 1) the agreement (bias) between fluorescence-based methods (NucleoCounter-SP100 [NC] vs. flow cytometry [FC]) for determining the viability (VIAB) of frozen/thawed stallion sperm; 2) the agreement between post-thaw sperm total motility (TMOT) and VIAB; 3) whether a difference between TMOT and VIAB [VIAB – TMOT] in frozen/thawed stallion sperm could be explained by
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Genetic basis of sow hyperprolificacy and litter size optimization based on a genome-wide association study Theriogenology (IF 2.8) Pub Date : 2024-02-01 Błażej Nowak, Anna Mucha, Magdalena Zatoń-Dobrowolska, Grzegorz Chrostowski, Wojciech Kruszyński
Over the last few decades, there has been a constant increase in sow litter size, the consequences of which include parturition duration extension, an increase in the percentage of stillborn and hypoxic piglets, and increased variation in piglet birth weight, which reduces their vitality. As such, it seems clear that further increasing sow fertility will generate difficulties and costs in rearing numerous
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Сopper nanoparticles supported on charcoal and betacellulin – Two novel stimulators of ovarian granulosa cell functions and their functional interrelationships Theriogenology (IF 2.8) Pub Date : 2024-02-01 Alexander V. Sirotkin, Barbora Loncová, Zuzana Fabová, Michaela Bartušová, Iris Martín-García, Abdel Halim Harrath, Francisco Alonso
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Genome-wide association and functional annotation analysis for the calving interval in Nellore cattle Theriogenology (IF 2.8) Pub Date : 2024-02-01 Mayra Silva Dias, Victor Breno Pedrosa, Valdecy Aparecida Rocha da Cruz, Marcio Ribeiro Silva, Luis Fernando Batista Pinto
Calving interval (CI) measures the number of days between two consecutive calves of the same cow, and previous studies based on phenotype and pedigree data reported low heritability for this trait. However, the genetic architecture of CI in the Nellore breed was not evaluated based on genomic data. Thus, this study aimed to estimate the heritability based on genomic data and carry out a genome-wide
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Oocyte electroporation prior to in vitro fertilization is an efficient method to generate single, double, and multiple knockout porcine embryos of interest in biomedicine and animal production Theriogenology (IF 2.8) Pub Date : 2024-02-01 Sergio Navarro-Serna, Celia Piñeiro-Silva, Irene Fernández-Martín, Martxel Dehesa-Etxebeste, Adolfo López de Munain, Joaquín Gadea
Genetically modified pigs play a critical role in mimicking human diseases, xenotransplantation, and the development of pigs resistant to viral diseases. The use of programmable endonucleases, including the CRISPR/Cas9 system, has revolutionized the generation of genetically modified pigs. This study evaluates the efficiency of electroporation of oocytes prior to fertilization in generating edited
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Blastocoel fluid aspiration improves vitrification outcomes and produces similar sexing results of in vitro-produced cattle embryos compared to microblade biopsy Theriogenology (IF 2.8) Pub Date : 2024-02-01 Iris Martínez-Rodero, Albert Salas-Huetos, Judith Diaz-Muñoz, Erika Alina Ordóñez-León, Tania García-Martínez, Marc Yeste, Carlos Olegario Hidalgo, Teresa Mogas
The potential applications of produced (IVP) cattle embryos are significantly enhanced when combined with genotype selection and cryopreservation techniques. While trophectoderm (TE) biopsies are frequently used for genotyping, cell-free DNA (cfDNA) found in blastocoele fluid (BF) arises as a less-invasive method. Moreover, the blastocoel collapse produced by BF aspiration could be beneficial for embryo
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Optimizing oocyte electroporation for genetic modification of porcine embryos: Evaluation of the parthenogenetic activation Theriogenology (IF 2.8) Pub Date : 2024-02-01 Celia Piñeiro-Silva, Joaquín Gadea
In reproductive biology, understanding the effects of novel techniques on early embryo development is of paramount importance. To date, the effects of electrical activation on oocytes prior to fertilization (IVF) are not well understood. The aim of this study was to investigate the effects of oocyte electroporation prior to IVF on embryo development and to differentiate between true embryos and parthenotes
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Sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle Theriogenology (IF 2.8) Pub Date : 2024-01-29 José María Sánchez, María Belén Rabaglino, Sandra Bagés-Arnal, Michael McDonald, Susanta K. Behura, Thomas E. Spencer, Pat Lonergan, Beatriz Fernandez-Fuertes
In cattle, mating to intact, but not vasectomised, bulls has been shown to modify the endometrial transcriptome, suggesting an important role of sperm in the modulation of the uterine environment in this species. However, it is not clear whether these changes are driven by intrinsic sperm factors, or by factors of accessory gland (AG) origin that bind to sperm at ejaculation. Therefore, the aim of
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Exposure to progesterone before an ovulation synchronization protocol increases the follicular diameter and fertility of multiparous suckled Bos taurus cows Theriogenology (IF 2.8) Pub Date : 2024-01-26 L.M.S. Simões, E.A. Lima, L.R. Carvalho, M.B.P. Martínez, G.M. Zanatta, M.F.O. Santos, A.B. Machado, M.M. Dias, B.M. Guerreiro, B.G. Freitas, M.R. Bastos, J.N.S. Sales
The objective of this study was to evaluate the effect of administering injectable progesterone (P4i) before a timed artificial insemination (TAI) protocol on the follicular growth, ovulation, and pregnancy rate of Bos taurus suckled cows. The effect of P4i administration before the TAI on the pregnancy rate (P/AI) was evaluated in 576 suckled Bos taurus cows at 30–90 days postpartum. In addition,
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Effects of commonly used carbamates (carbaryl and thiram) on the regulatory, secretory and motor functions of bovine cervixes in vitro Theriogenology (IF 2.8) Pub Date : 2024-01-26 Wrobel Michal Hubert, Mlynarczuk Jaroslaw, Rekawiecki Robert
Previously studied classes of pesticides, including organochlorines, organophosphates and pyrethroids disturb the mechanism that causes bovine myometrial contractions. Hence, the aim of this study was to investigate the effects of carbaryl and thiram, which are representative carbamate pesticides commonly used in global agriculture, on the motor and secretory functions of bovine cervixes. Additionally
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Analysis of production efficiency of cloned transgenic Yucatan miniature pigs according to recipient breeds with embryo transfer conditions Theriogenology (IF 2.8) Pub Date : 2024-01-24 Jun-Hyeong Kim, Joohyun Shim, Nayoung Ko, Hyoung-Joo Kim, Yongjin Lee, Kimyung Choi
The purpose of this study was to compare the efficiency of the production of cloned transgenic Yucatan miniature pigs (YMPs) using two recipient breeds, i.e., YMPs and domestic pigs (DPs), under various embryo transfer conditions. We initially assessed the in vitro developmental competence of embryos obtained via somatic cell nuclear transfer (SCNT) from three different transgenic donor cells. No difference
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Utilising male stimulus to improve the reproductive efficiency of 8-month-old nulliparous ewes and adult parous ewes Theriogenology (IF 2.8) Pub Date : 2024-01-21 César A. Rosales-Nieto, Andrew N. Thompson, Venancio Cuevas-Reyes, Luisa E.S. Hérnandez-Arteaga, Johan C. Greeff, Richard Ehrhardt, Almudena Veiga-Lopez, Graeme B. Martin
We tested whether utilising the male effect to stimulate ewes before the mating period can reduce the time to conception following the introduction of entire rams, and increase fertility, prolificacy, and reproductive rate (number of fetuses per 100 ewes exposed to fertile rams). A retrospective analysis was used to analyse records from 59,716 ewes collected over 34 years (1986–2020) from seven genotypes:
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Combined analysis of temporal metabolomics and transcriptomics reveals the metabolic patterns in goat oocytes during maturation Theriogenology (IF 2.8) Pub Date : 2024-01-26 Yixin Ma, Wei Zhang, Ming Gao, Jiashuo Li, Qiang Wang, Minjian Chen, Ling Gu
Well-balanced and orderly metabolism is a crucial prerequisite for promoting oogenesis. Involvement of single metabolites in oocyte development has been widely reported; however, the comprehensive metabolic framework controlling oocyte maturation is still lacking. In the present study, we employed an integrated temporal metabolomic and transcriptomic method to analyze metabolism in goat oocytes at
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Effects of salinity on pre- and post-fertilization developmental events in the mangrove oyster Crassostrea rhizophorae (GUILDING, 1828) Theriogenology (IF 2.8) Pub Date : 2024-01-26 Rodolf Gabriel Prazeres Silva Lopes, Ana Paula Rego, Sabrina Melo de Jesus Gomes, Ícaro G. Antonio, Thaís Brito Freire, Maria Raquel Moura Coimbra
The mangrove oyster Crassostrea rhizophorae is identified as a potentially valuable species for tropical aquaculture, however, information on the physiological mechanisms of reproduction under laboratory conditions for this species is limited. This study investigated the effects of salinity at different concentrations (15, 20, 25, 30, 35, and 40 g/L) on the induction of germinal vesicle breakdown (GVBD)
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Follicular fluid exosomes inhibit expression of BTG2 and promote glucose uptake in granulosa cells by delivering miR-21-5p Theriogenology (IF 2.8) Pub Date : 2024-01-26 Xue Chen, Maosheng Cao, Chenfeng Yuan, Yuxin Luo, Nan Wang, Kening Liu, Tong Chen, Lu Chen, Boqi Zhang, Chunjin Li, Xu Zhou
Glucose metabolism in granulosa cells (GCs) is essential for follicle development and oocyte maturation. Porcine follicular fluid exosomes promote the proliferation of porcine GCs and the synthesis of steroid hormones. However, their role in regulating glucose uptake in GCs is unclear. The objective of this study was to elucidate the effects of porcine follicular fluid exosomes on glucose uptake in
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Is estradiol valerate an alternative to estradiol benzoate in promoting the synchronization of ovulation and timed artificial insemination in suckled Bos indicus beef cows? Theriogenology (IF 2.8) Pub Date : 2024-01-26 José Nélio de Sousa Sales, Matheus Pedroso Vicente, Laís Reis Carvalho, Lucas Araújo Lemos, Luiz Manoel Souza Simões, Marcos Henrique Alcantara Colli, Walter Antônio Gonçales Junior, Henderson Ayres, Lais Mendes Vieira, Rodolfo Daniel Mingoti, João Paulo Barbuio
The objective of the present study was to evaluate the effect of estradiol valerate administered at the beginning of the ovulation synchronization protocol on the pregnancy rate of Bos indicus cows. In the experiments, the following products from MSD, Sao Paulo, Brazil were used: estradiol valerate (EV), estradiol benzoate (EB), intravaginal progesterone device (P4), estradiol cypionate (EC), equine