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Finding a needle in a haystack: DNA Haemoproteus columbae enrichment using percoll density gradient and flow cytometry Vet. Parasitol. (IF 2.6) Pub Date : 2024-03-12 Brayan Andrés Gamboa-Suárez, Ingrid Astrid Lotta-Arévalo, Felipe Sarmiento Salazar, Nubia E. Matta
Isolation of genomic DNA of blood parasites in birds, reptiles, amphibians, and fishes is a challenging task, given that their red blood cells are nucleated; for that reason, parasite genomic DNA is only a fraction of the total extracted DNA, and it is challenging to obtain concentrated high-quality genetic material. Percoll Density Gradient (PDG) and flow cytometry are tools for separating and analyzing
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Moniezia benedeni infection increases IgE+ cells in sheep (Ovis aries) small intestine Vet. Parasitol. (IF 2.6) Pub Date : 2024-03-12 Jing Pan, Wan-Ling Yao, Li-Ping Liu, Bao-Shan Wang, Wen-Zhu Chai, Zhen Huang, Xi-Ping Fan, Wan-Hong He, Wen-Hui Wang, Wang-Dong Zhang
The concentration of immunoglobulin (Ig) E is the lowest among serum Igs, but it can induce type I hypersensitivity and plays an important role in anti-parasitic infection. The present study aimed to explore the residence characteristics of IgE cells in the sheep small intestine and the impact of infection on them. The recombinant plasmids pET-28a-IgE were constructed and induced and expressed in (DE3)
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Antiparasitic efficacy of flavonoids identified from Psoralea corylifolia against Tetrahymena piriformis in guppy (Poecilia reticulate) Vet. Parasitol. (IF 2.6) Pub Date : 2024-03-11 Hongming Tang, Sicong Guo, Hongxin Xue, Zhixin Guo, Yihao Li, Qinghua Yu, Yuxiao Liu, Weijia Zhou, Shigen Ye
belongs to the ciliated protists (ciliates), causing severe economic losses in aquaculture. Chemical drugs currently used usually have toxic side effects, and there is no specific drug against . Therefore, it is an urgent need to identify new antiparasitic lead compounds. In the present study, the in vitro parasiticidal activity of ethyl acetate (EtOAc) extracts and water extracts from 22 selected
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Growth hormone stimulates the in vitro development and establishment of Haemonchus contortus in sheep Vet. Parasitol. (IF 2.6) Pub Date : 2024-03-08 Adolfo Sánchez-Paredes, César Cuenca-Verde, María Guadalupe Prado-Ochoa, Jorge Morales-Montor, Fernando Alba-Hurtado, Marco Antonio Muñoz-Guzmán
The physiologic increase in some sex hormones has been associated with an increase in the parasite load caused by in ewes, especially prolactin. In lambs that are especially susceptible to hemonchosis, the levels of sex hormones are low; in contrast, the levels of another pituitary hormone, growth hormone (GH), which is structurally very similar to prolactin, are high. In this study, the and effects
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A small proportion of Zebu genetic background in crossbred calves may not be enough to improve resistance against natural bovine Babesia spp. infections Vet. Parasitol. (IF 2.6) Pub Date : 2024-03-08 Bianca Tainá Azevedo, Henrique Nunes de Oliveira, Luciana Morita Katiki, Anibal Eugênio Vercesi Filho, Ana Gonçalves Domingos, Sandra Antunes, Cintia Hiromi Okino, Márcia Cristina de Sena Oliveira, Adriana Mércia Guaratini Ibelli, Rodrigo Giglioti
The main objective of cattle breeders in tropical and subtropical regions is to acquire animals with taurine-productive traits adapted to the broad weather range of these regions. However, one of the main challenges on using taurine genetics in these areas is the high susceptibility of these animals to tick-borne diseases. Consequently, the present study evaluated from 10 November 2021 to 19 April
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Association of β-globin polymorphisms and tolerance to haemonchosis in ewes and lambs of different sheep breeds Vet. Parasitol. (IF 2.6) Pub Date : 2024-03-07 Rafaela Tami Ikeda Kapritchkoff, Cintia Hiromi Okino, Simone Cristina Méo Niciura, Hornblenda Joaquina Silva Bello, Renata Silva Matos, Glaucia Roberta Melito, Flavia Aline Bressani, Sérgio Novita Esteves, Ana Carolina de Souza Chagas
Gastrointestinal nematodes (GIN), especially , represent a significant challenge for sheep production Given the global concern about GIN anthelmintic resistance, alternative control methods able to reduce the dependence on these drugs are highly advisable. Since previous studies have shown that sheep carrying the Hb-A allele of β-globin are more resistant to , this study aimed to investigate the relationship
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Entomopathogenic fungi with biological control potential against poultry red mite (Dermanyssus gallinae, Arachnida: Dermanyssidae) Vet. Parasitol. (IF 2.6) Pub Date : 2024-03-02 Miraç Bayramoğlu, Zeynep Bayramoğlu, Levent Aydın, Suna Aslı Zengin, Veli Yılgör Çırak, Zihni Demirbağ, İsmail Demir
The poultry red mite, (Arachnida: Dermanyssidae) is a pest that causes significant economic loss in laying hens for which control methods are limited. In this study, the effects of 20 indigenous fungal strains on poultry red mites in chicken farms were investigated. All experiments were conducted under laboratory condition at 28 ± 1 °C and 80 ± 5% humidity. A screening test showed that strain As-2
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Cloning, expression, and functional identification of aquaporin genes from Eimeria tenella Vet. Parasitol. (IF 2.6) Pub Date : 2024-03-01 Jing Wang, Zigang Qu, Xueting Ma, Joshua Seun Olajide, Jianping Cai
Avian coccidiosis, caused by spp., is one of the major parasitic diseases in chicken. Aquaporins (AQP) are essential mediators of water regulation and nutritional intake in parasites, and it may be a suitable molecule for chemotherapeutic target and vaccine candidate. We identified two aquaporin genes in (EtAQP1 and EtAQP2) with their full sequence, and the expression profiles were analyzed across
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Characterisation of protective vaccine antigens from the thiol-containing components of excretory/secretory material of Ostertagia ostertagi Vet. Parasitol. (IF 2.6) Pub Date : 2024-03-01 Daniel R.G. Price, Philip Steele, David Frew, Kevin McLean, Dorota Androscuk, Peter Geldhof, Jimmy Borloo, Javier Palarea Albaladejo, Alasdair J. Nisbet, Tom N. McNeilly
Previous vaccination trials have demonstrated that thiol proteins affinity purified from excretory-secretory products ( ES-thiol) are protective against homologous challenge. Here we have shown that protection induced by this vaccine was consistent across four independent vaccine-challenge experiments. Protection is associated with reduced cumulative faecal egg counts across the duration of the trials
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Performance of three techniques for diagnosing equine tapeworm infection Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-24 Hailey C. Anderson, Shaelin F. Warner, Nichol E. Ripley, Martin K. Nielsen
Tapeworm infection in horses can cause serious health concerns, and recent data have documented treatment failures in the most common species, . The threat of anthelmintic resistance in is of particular concern because of poor diagnostic performance of standard egg counting techniques for detecting this parasite. This study compared the performance of three diagnostic techniques 1) Mini-FLOTAC, 2)
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Rabbits as reservoirs: An updated perspective of the zoonotic risk from Cryptosporidium and Giardia Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-24 Siobhon Egan, Amanda D. Barbosa, Yaoyu Feng, Lihua Xiao, Una Ryan
Rabbits are highly abundant in many countries and can serve as reservoirs of diseases for a diversity of pathogens including the enteric protozoan parasites, and . Both parasites shed environmentally robust environmental stages (oo/cysts) and have been responsible for numerous waterborne outbreaks of diseases. and are responsible for most infections in humans, while assemblages A and B, cause most
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Association between Opisthorchis viverrini infection in cats and humans: Non-spatial and spatial analyses Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-22 Pornphutthachat Sota, Songkiad Upontain, Sirikachorn Tangkawattana, Veerasak Punyapornwithaya, Kanchana Nakhapakorn, Banchob Sripa
Cats are recognized as significant reservoir hosts for human opisthorchiasis, particularly in areas with a high prevalence of infection. Despite this, the precise role of cats in the transmission of between humans and felines remains unclear. This study investigates the association between these two hosts through both spatial and non-spatial analyses in the endemic Thanya sub-district of Thailand.
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Polymeric films of corn starch enhance the lethal effects of thymol and carvacrol terpenes upon Rhipicephalus microplus ticks Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-20 Olga O. Anjos, Matheus N. Gomes, Caio P. Tavares, Dauana M. Sousa, Caritas J.S. Mendonça, José Reck, Adeilton P. Maciel, Livio M. Costa-Junior
The tick is a parasite of great importance in cattle breeding. It is responsible for huge economic losses. The application of synthetic acaricides is used as a form of control. However, resistant strains have been selected over the years, making it necessary to search for new alternative formulations. The present study aimed to formulate biodegradable films impregnated with the terpenes carvacrol and
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Investigating the perceived versus actual gastrointestinal nematode challenge on extensive sheep farms Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-13 Eilidh Geddes, Claire Morgan-Davies, Ann McLaren, Philip J. Skuce, Jade M. Duncan, Neil Sargison, Fiona Kenyon
Extensive farming systems form an integral part of sheep production systems across Europe. However, with innate production handicaps, declining sheep numbers and narrow economic margins, production is becoming increasingly challenging threatening the future sustainability of the industry. Gastrointestinal nematodes (GINs) are a significant cause of production losses to the global sheep industry, with
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Effects of natural extract from medicinal herbs on broilers experimentally infected with Eimeria tenella Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-12 Yuanyuan Jiang, Yujuan Zeng, Keyu Chen, Haoran Cheng, Songjiang Dai, Xuexue Deng, Long Wang, Jinqiu Liao, Ruiwu Yang, Li Zhang
This study aimed to evaluate the effects of natural extracts from nine medicinal herbs (SMA) on the growth performance, immunity, and intestinal integrity of broilers experimentally infected with . A total of 252 one-day-old broiler chicks were divided into 7 groups with 3 replicates per group and 12 broilers per cage. The groups were uninfected–untreated blank control group (BC), infected–untreated
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The proof is in the poo-ding: Benefits of the longitudinal molecular surveillance of drug resistance demonstrated in a New South Wales cattle herd Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-12 Emily Kate Francis, Jan Šlapeta
Our understanding of anthelmintic resistance in the gastrointestinal nematodes of Australian cattle relies exclusively on small-scale phenotypic reports utilising traditional faecal egg count reduction tests. This approach is not readily scalable to establish the national prevalence of resistance, nor is it conducive of routine longitudinal surveillance for the emergence of resistance in its early
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Molecular detection and characterization of Blastocystis in herbivore livestock species in Portugal Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-12 Ana M. Figueiredo, Mónica Santín, Pamela C. Köster, Alejandro Dashti, Jenny G. Maloney, Rita T. Torres, Carlos Fonseca, Atle Mysterud, João Carvalho, Dário Hipólito, Mariana Rossa, Josman D. Palmeira, David González-Barrio, Rafael Calero-Bernal, David Carmena
is a ubiquitous intestinal protist in humans and animals worldwide. The traditional livestock free-roaming raising system in rural communities increases the risk of infection with contact with a wider range of pathogens transmitted via the faecal-oral route associated with that wildlife-livestock-human interface. However, no studies have been conducted to determine the occurrence and subtype distribution
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Efficacy of eprinomectin 5 mg/mL topical solution administered pour on at 1 mg per kg body weight against Oestrus ovis myiasis in sheep and goats Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-09 Steffen Rehbein, Elias Papadopoulos, Konstantinos Arsenopoulos, Zvezdelina Kirkova, Petar Iliev, Renate Rauh, Becky Fankhauser
The larvae of the nasal bot, , mainly parasitize sheep and goats and some species of wild Caprinae but other mammals and humans are also vulnerable to infestation. Eprinomectin 5 mg/mL topical solution (EPRINEX® Multi, Boehringer Ingelheim) administered at 1 mg eprinomectin per kg body weight pour on was recently authorized as an anthelmintic for sheep and goats with zero hours milk withdrawal in several
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Faecal egg count reduction test in goats: Zooming in on the genus level Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-08 Anna Maurizio, Lucie Škorpíková, Jana Ilgová, Cinzia Tessarin, Giorgia Dotto, Nikol Reslová, Jaroslav Vadlejch, Erica Marchiori, Antonio Frangipane di Regalbono, Martin Kašný, Rudi Cassini
The faecal egg count reduction test (FECRT) is the most widely used method to assess treatment efficacy against gastrointestinal nematodes (GIN). Information on genera composition of the GIN community is not available with this test and it is commonly obtained by identifying cultured third-stage larvae (L3) or through molecular assays in the post-treatment survey, but results provided are usually only
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Prediction of coccidiosis prevalence in extensive backyard chickens in countries and regions of the Horn of Africa Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-03 Violeta Muñoz-Gómez, Reinhard Furrer, Jie Yin, Alexandra PM Shaw, Philip Rasmussen, Paul R. Torgerson
Coccidiosis is one of the leading morbidity causes in chickens, causing a reduction of body weight and egg production. Backyard chickens are at risk of developing clinical and subclinical coccidiosis due to outdoor housing and scavenging behaviour, jeopardizing food security in households. The objectives of this study were to estimate clinical prevalence of coccidiosis at country and regional levels
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Immunoprotective effects of DNA vaccine against Eimeria tenella based on EtAMA3 and EtRON2L2 Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-03 Xiangjie Chen, Xixi Qiu, Junli Ni, Shenquan Liao, Nanshan Qi, Juan Li, Minna Lv, Xuhui Lin, Haiming Cai, Junjing Hu, Yongle Song, Lijun Yin, Yibin Zhu, Jianfei Zhang, Haoji Zhang, Mingfei Sun
is the most pathogenic and harmful intestinal parasitic protozoan. Recombinant DNA vaccines open options for promising strategies for preventing avian coccidiosis, replacing chemical drugs and live oocyst vaccines. Two important antigenic proteins, EtAMA3 (also known as SporoAMA1) and EtRON2, act together to promote the invasion of sporozoites. In this study, a recombinant DNA vaccine, designated pcDNA3
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In-herd prevalence of Fasciola hepatica and Calicophoron / Paramphistomum spp. infections in German dairy cows with comparison of two coproscopical methods and establishment of real-time pyrosequencing for rumen fluke species differentiation Vet. Parasitol. (IF 2.6) Pub Date : 2024-02-01 Anna Sophie Hecker, Marie-Kristin Raulf, Sven König, Gabriela Knubben-Schweizer, Christoph Wenzel, Katharina May, Christina Strube
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*Inactivation of encysted muscle larvae of Trichinella spiralis in pigs using Mebendazole Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-26 Jorrell Fredericks, Dolores E. Hill, Dante S. Zarlenga, Valsin M. Fournet, Diane S. Hawkins-Cooper, Joseph F. Urban Jr., Matthew Kramer
We evaluated the effect of 4 anthelmintic treatments on the viability of encysted muscle larvae (ML) 55 days post infection (PI) in experimentally infected pigs. Muscle larvae were isolated from pig muscle by artificial digestion after oral treatment of pigs with Levamisole (8 mg/kg, daily for 5 days) and Mebendazole (50 mg/kg, daily for 5 days); Doramectin (0.3 mg/kg, single IM injection), and Moxidectin
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Rhipicephalus microplus thyropin-like protein: Structural and immunologic analyzes Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-26 Lucas Andre Dedavid e Silva, Luís Fernando Parizi, Franciéli Adriane Molossi, David Driemeier, Itabajara da Silva Vaz Junior
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In Vitro anthelmintic efficacy of medicinal plant essential oils against Marshallagia marshalli: Evidence on oxidative/nitrosative stress biomarkers, DNA damage, and egg hatchability Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-24 Yousef Ahmadzadeh naghadeh, Farnaz Malekifard, Bijan Esmaeilnejad
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Comparison of Giardia duodenalis point-of-care antigen faecal tests to reference laboratory assays in non-symptomatic dogs Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-24 Stella Ktenas, Florian Roeber, Maira N. Meggiolaro, Athena Ktenas, Michael P. Ward, Jan Šlapeta
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Activation of CncC pathway by ROS burst regulates ABC transporter responsible for beta-cypermethrin resistance in Dermanyssus gallinae (Acari:Dermanyssidae) Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-21 Penglong Wang, Huan Li, Jiali Meng, Qi Liu, Xu Wang, Bohan Wang, Boxing Liu, Chuanwen Wang, Weiwei Sun, Baoliang Pan
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Acaricidal activity of synthetic spilanthol derivative against ticks of medical and veterinary importance Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-20 Luis Adriano Anholeto, Guilherme Andrade Brancaglion, Danilo Martins dos Santos, Rafaela Tami Ikeda Kapritchkoff, Karina Neoob de Carvalho Castro, Kirley Marques Canuto, Rodney Alexandre Ferreira Rodrigues, Daniel Souza Correa, Ana Carolina de Souza Chagas, Julio Cezar Pastre
The ANESPSAT, a synthetic spilanthol derivative, and its nanoformulation were evaluated against and ticks. ANESPSAT activity was compared with spilanthol and derivatives (ANESPE and others). The compound was synthesized in a gram-scale by a 2-step process, comprising a direct ester amidation and a Horner-Wadsworth- Emmons reaction. The nanoemulsions were produced by coarse homogenization followed by
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Adapting the in vitro rumen incubation method to evaluate the effect of a plant extract on the exsheathment inhibition of Haemonchus contortus infective larvae Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-20 Cindy Goretti Marin-Tun, María Gabriela Mancilla-Montelongo, Concepción Manuela Capetillo-Leal, Carlos Alfredo Sandoval-Castro, Hervé Hoste, Rocío Borges-Argáez, Juan Felipe de Jesús Torres-Acosta
This study adapted the rumen incubation (IVRI) method to evaluate the biological activity of a leaves extract against the exsheathment of infective larvae (L), and to determine the role of plant polyphenols on the biological activity. The incubation protocol followed the IVRI method, adding polyethylene glycol (PEG) as a polyphenol-blocking agent. The L were incubated in ruminal liquor (RL), ruminal
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Efficacy and safety of geranium-oregano-thymol formulations to control of dog tick Rhipicephalus sanguineus sensu lato under laboratory and field conditions Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-19 Sahar M. Gadelhaq, Samar M. Ibrahium, Abdel-Azeem S. Abdel-Baki, Waleed M. Arafa, Saleh Al-Quraishy, Ahmed O. Hassan, Noha H. Abdelgelil, Manal Ahmed, Shawky M. Aboelhadid
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Evaluation of LAMP for Fasciola hepatica detection from faecal samples of experimentally and naturally infected cattle Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-19 Tanjina Bari, Md. Abdullah Al Mamun, Hayley Toet, Vignesh Rathinasamy, Jo-Ann Larkins, Travis Beddoe, Terry W. Spithill, David Piedrafita, Andrew R. Greenhill
causes liver fluke disease in production animals and humans worldwide. Faecal egg counts (FEC) are the most common diagnostic tool for the diagnosis of liver fluke disease. However, FEC has low sensitivity and is often unreliable for the detection of patent infection. In this study, loop-mediated isothermal amplification (LAMP) was optimised and evaluated for the detection of infection, with the aim
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Metaphylactic strategies using toltrazuril against coccidiosis in goat kids Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-19 Aránzazu C. Guedes, Magnolia Conde-Felipe, Emilio Barba, José Manuel Molina, María del Carmen Muñoz, Otilia Ferrer, Sergio Martín, Carlos Hermosilla, Anja Taubert, Antonio Ruiz
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CRISPR-Cas9-based method for isolating microgametes of Eimeria tenella Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-18 Zigang Qu, Zhenxing Gong, Joshua Seun Olajide, Jing Wang, Jianping Cai
infections are known to cause severe caecal damage and death of the infected chicken. Gamogony is an essential stage in life cycle and in the establishment of coccidiosis. Prior research had extensively explored isolation and separation of the parasite gametes - microgamete (male) and macrogamete (female). However, there is little information on the efficient, highly purified and distinctly separated
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Preparation and application of biocontrol formulation of nematode-trapping fungus-Duddingtonia flagrans Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-18 Luyao Hao, Yuan Guo, Xiaomin Wang, Mingjuan Gao, Tonghai Liu, Yuan Ma, Yanni Zhang, Qiannan Li, Rui Wang, Xihuo You
The use of nematophagous fungi as a biological control strategy for parasitic gastrointestinal nematodes (GINs) in livestock holds promise as an innovative alternative approach. This study aimed to evaluate the clinical efficacy of a lyophilized preparation, utilized in association with the anthelmintics ivermectin or albendazole, to control GINs in Tibetan sheep on a farm based in Qinghai Province
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Refugia, climatic conditions and farm management factors as drivers of adaptation in Nematodirus battus populations Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-17 Lynsey A. Melville, Giles Innocent, Jan Van Dijk, Sian Mitchell, David J. Bartley
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Nationwide USA re-analysis of amplicon metabarcoding targeting β-tubulin isoform-1 reveals absence of benzimidazole resistant SNPs in Ancylostoma braziliense, Ancylostoma tubaeforme and Uncinaria stenocephala Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-17 Thomas Stocker, Michael P. Ward, Jan Šlapeta
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Neospora caninum peroxiredoxin 1 is an essential virulence effector with antioxidant function Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-17 Yutao Shao, Xiaodan Yuan, Boya Du, Xuancheng Zhang, Xin Li, Xu Zhang, Pengtao Gong, Nan Zhang, Xiaocen Wang, Jianhua Li
an obligate intracellular parasitic protozoan discovered by Dubey in 1988, is the pathogen of neosporosis, which causes neurological symptoms in dogs and abortions in cows. Since there is no effective drug or vaccine against , a deeper understanding of the molecules critical to parasite survival inside host cells is necessary. This study aimed to determine the role of peroxiredoxin 1 (NcPrx1) in maintaining
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RPA-CRISPR/Cas9-based method for the detection of Toxoplasma gondii: A proof of concept Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-14 Mengchen Wu, Haiyan Wu, Xueqiu Chen, Fei Wu, Guangxu Ma, Aifang Du, Yi Yang
is a widespread and specialized intracellular protozoan pathogen that affects one third of the world’ s population, posing a great threat to public health. As the definitive host, cats excrete oocysts and play a crucial role in the transmission of toxoplasmosis. The current diagnostic tools usually require bulky equipment and expertize, which hinders the efficient diagnosis and intervention of infection
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Worm control practices used by Thoroughbred horse managers in Australia: A national survey Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-12 Ghazanfar Abbas, Jenni Bauquier, Anne Beasley, Caroline Jacobson, Charles El-Hage, Edwina J.A. Wilkes, Peter Carrigan, Lucy Cudmore, John Hurley, Ian Beveridge, Martin K. Nielsen, Kristopher J. Hughes, Mark A. Stevenson, Abdul Jabbar
This study assessed worm control practices used by Australian Thoroughbred farm managers with an online questionnaire survey. The questionnaire comprised 52 questions (close-ended: 44; open-ended: 8) about farm demography and general husbandry practices, farm managers’ knowledge of gastrointestinal nematodes (GIN) and their importance, diagnosis, worm control strategies and anthelmintics, anthelmintic
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Fosfatriclaben, a prodrug of triclabendazole: Preparation, stability, and fasciolicidal activity of three new intramuscular formulations Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-12 Miguel Flores-Ramos, Gerardo Leyva-Gómez, Tania Rojas-Campos, Irene Cruz-Mendoza, Alicia Hernández-Campos, Yolanda Vera-Montenegro, Rafael Castillo, Israel Velázquez-Martínez, Cecilia Padierna-Mota, Rosa Arias-García, Froylán Ibarra-Velarde
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Efficacy of a spot-on formulation containing 280 mg/ml fluralaner and 14 mg/ml moxidectin (Bravecto® Plus) in the prevention of cat aelurostrongylosis under field conditions Vet. Parasitol. (IF 2.6) Pub Date : 2024-01-11 Angela Di Cesare, Simone Morelli, Nadja Rohdich, Zvezdelina Kirkova, Balázs Capári, Wolfgang Loehlein, Oliver Wolf, Eva Zschiesche, Mariasole Colombo, Chiara Astuti, Donato Traversa
is the most important respiratory nematode of domestic cats. Effective control options are crucial to protect health and welfare of cats and to reduce the spread of aelurostrongylosis in both enzootic and free regions. The present study evaluated the efficacy of a spot-on formulation containing 280 mg/ml fluralaner and 14 mg/ml moxidectin (Bravecto® Plus, MSD) in the prevention of aelurostrongylosis
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Gastrointestinal parasite community structure in horses after the introduction of selective anthelmintic treatment strategies Vet. Parasitol. (IF 2.6) Pub Date : 2023-12-25 Peter Halvarsson, Giulio Grandi, Sara Hägglund, Johan Höglund
A relatively new method to study the species richness and diversity of nematode parasites in grazing animals is to perform deep sequencing on composite samples containing a mixture of parasites. In this work, we compared species composition of strongyles in two groups of horses as a function of egg count and age, based on a DNA barcoding approach. Faecal egg counts and larval cultures were obtained
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Stochastic modelling to predict a priori the potential outcomes of different control schemes against the cattle tick Rhipicephalus microplus Vet. Parasitol. (IF 2.6) Pub Date : 2023-12-21 Camila Miotti, Nicolás Morel, Marcelo Lisandro Signorini, Santiago Nava
Stochastic models are valuable tools to describe and analyze tick population dynamic in a given area, and to evaluate different control schemes. The objective of this study was to provide a tool to predict a priori how a control scheme could affect the abundance of Rhipicephalus microplus in an area highly favourable for its development, through the building of a stochastic model of the population
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Efficacy of afoxolaner or the combination of afoxolaner with milbemycin oxime against Otodectes cynotis in naturally infested dogs Vet. Parasitol. (IF 2.6) Pub Date : 2023-12-20 Georgios Sioutas, Elias Papadopoulos, Maxime Madder, Frederic Beugnet, Eric Tielemans
Otodectes cynotis, commonly known as "the ear mite," is a highly contagious ectoparasite and a significant cause of otitis externa in canines. The objective of the current study was to determine the efficacy of the isoxazoline afoxolaner (Nexgard®), and the combination of afoxolaner with milbemycin oxime (Nexgard Spectra®), in dogs naturally infested with O. cynotis. In total, 32 infested client-owned
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Ponazuril: Clinical efficacy, ultrastructure, and histopathology studies of in vivo anticoccidial action against Eimeria tenella Vet. Parasitol. (IF 2.6) Pub Date : 2023-12-18 Yanyang Yuan, Li’nan Yang, Qingyu Zhao, Lei Xiong, Ming Yang, Xun Suo, Zhihui Hao
Ponazuril, a novel antiprotozoal drug in the class of triazine, has shown a promising application on apicomplexan infections in poultry and livestock. However, the effect and mechanism of action of ponazuril against Eimeria tenella (E. tenella) are unclear. The efficacy against E. tenella was initially studied by administering different doses of ponazuril in drinking water. The treated stage and site
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Exploring multiplex qPCR as a diagnostic tool for detecting microfilarial DNA in dogs infected with Dirofilaria immitis: A comparative analysis with the modified Knott’s test Vet. Parasitol. (IF 2.6) Pub Date : 2023-12-14 Daisy Ching-Wai Lau, Rosemonde Isabella Power, Jan Šlapeta
Current recommendations to diagnose cardiopulmonary dirofilariosis in dogs caused by Dirofilaria immitis involves tandem antigen and circulating microfilariae tests. The modified Knott’s test is an important tool in heartworm diagnosis, allowing identification of circulating microfilariae. However, the subjective nature of the modified Knott’s test affects its accuracy and diagnostic laboratories usually
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Genetic variability of Dirofilaria repens isolates from humans and dogs in Italy Vet. Parasitol. (IF 2.6) Pub Date : 2023-12-12 Simona Gabrielli, Leonardo Brustenga, Giulia Morganti, Lavinia Ciuca, Giulia Barlozzari, Giulia Rigamonti, Margherita Orlandi, Monica Sforna, Fabrizia Veronesi
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Understanding temporal and spatial distribution of intestinal nematodes of horses using faecal egg counts and DNA metabarcoding Vet. Parasitol. (IF 2.6) Pub Date : 2023-12-03 Ghazanfar Abbas, Abdul Ghafar, Anne Beasley, Mark A. Stevenson, Jenni Bauquier, Anson V. Koehler, Edwina J.A. Wilkes, Emma McConnell, Charles El-Hage, Peter Carrigan, Lucy Cudmore, John Hurley, Charles G. Gauci, Ian Beveridge, Caroline Jacobson, Martin K. Nielsen, Kristopher J. Hughes, Abdul Jabbar
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Bayesian hierarchical modelling of the geospatial distribution of fasciolosis in dairy cattle and the impact on production: Application to the main milk-producing region (Galicia) in Spain Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-30 Marta González-Warleta, José Antonio Castro-Hermida, Mario Figueira, Jesúa López, David Conesa, Antonio López-Quílez, Florencio M. Ubeira, Mercedes Mezo
Fasciolosis caused by Fasciola hepatica is a common parasitic infection among cattle in many countries. Although infected adult cows rarely show overt clinical signs, milk production may be impaired. Thus, significant production losses may occur in dairy herds with a high prevalence of fasciolosis. In this study, Bayesian hierarchical modelling was used to estimate the geospatial distribution of dairy
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Distribution and prevalence of antibodies to Trichinella spp. and Toxoplasma gondii in wild pigs (Sus scrofa) in the United States Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-28 Christopher A. Cleveland, Ellen Haynes, Katherine C. Callaghan, Alinde Fojtik, Sarah Coker, Emily Doub, Vienna R. Brown, Ania A. Majewska, Michael J. Yabsley
Invasive wild pigs (Sus scrofa) are a reservoir for over 100 viral, bacterial, and parasitic pathogens that are transmissible to humans, livestock, domestic animals, and wildlife in North America. Numerous historical local surveys and results from a nation-wide survey (2006–2010) indicated that wild pigs in the United States act as reservoirs for Trichinella spp. and Toxoplasma gondii, two zoonotic
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Babesia bovis RON2 binds to bovine erythrocytes through a highly conserved epitope Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-28 Laura Cuy-Chaparro, Danny Barney-Borrero, Gabriela Arévalo-Pinzón, César Reyes, Darwin Andrés Moreno-Pérez, Manuel Alfonso Patarroyo
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Wing geometric morphometrics and DNA barcoding to distinguish three closely related species of Armigeres mosquitoes (Diptera: Culicidae) in Thailand Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-28 Sedthapong Laojun, Tanasak Changbunjong, Suchada Sumruayphol, Siripong Pimsuka, Tanawat Chaiphongpachara
Armigeres subalbatus, a mosquito species widely found in Thailand and other Asian countries, serves as a vector for filarial parasites, affecting both humans and animals. However, the surveillance of this vector is complicated because of its morphological similarity to two other species, Armigeres dohami and Armigeres kesseli. To differentiate these morphologically similar species, our study employed
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The distribution of strongylid egg and lungworm (Dictyocaulus eckerti) larval counts in adult female farmed red deer (Cervus elaphus), and the implications for parasite control Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-23 Alex Chambers, Chris Miller, Peter Green, Paul Candy, Dave Leathwick
Whilst healthy adult farmed red deer show little clinical indication of parasite infection, they may still be maintaining infection levels on the farm through low-level shedding of nematode eggs and lungworm larvae. This work was undertaken to establish the long-term distribution of parasite counts, to determine whether the higher counts seen in previous trials are repeatable across the same animals
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Simultaneous resistance to multiple anthelmintic classes in nematode parasites of cattle in New Zealand Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-19 Christian Sauermann, Tania Waghorn, Chris Miller, Dave Leathwick
Resistance to the benzimidazole and macrocyclic lactone anthelmintics is widespread in Cooperia spp. on cattle farms in New Zealand. Since this was first documented in 2006 little has changed in cattle farming systems except for the widespread use of levamisole to control Cooperia spp. in young cattle (i.e., parasite control has maintained an almost total reliance on use of anthelmintics). Here we
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Reproductive and margin of safety of a fixed-dose combination injectable endectocide (0.2 mg/kg doramectin; 6.0 mg/kg levamisole hydrochloride) in cattle Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-10 Matthew J. Krautmann, Matthew Edmonds, Jenifer Edmonds, Nicholas K. Van Engen, Kevin Esch, Rodney K. Frank, Erin Quist, Jezaniah Kira Tena, Kayla M. Saad, Noelle Cotey, Andrew A. DeRosa
We present a fixed-dose combination injectable (FDCI) solution for cattle formulated for a single subcutaneous administration at a dose rate of 1 ml/25 kg of body weight to deliver a dose of 0.2 mg/kg of doramectin and 6.0 mg/kg of levamisole hydrochloride (5.1 mg/kg base equivalent). This drug product is marketed in the United States under the tradename Valcor® and in Australia and New Zealand under
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miRNA based biomarkers for the early diagnosis of Echinococcus granulosus in experimentally infected dogs Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-13 Figen Celik, Ahmet Tektemur, Sami Simsek
Cystic echinococcosis, which is caused by the Echinococcus granulosus. Carnivores, as final hosts, contain adult tapeworms in the small intestine, and a variety of mammals, including humans, harbor the metacestod. This study was designed to investigate the miRNA-based biomarkers for early and accurate diagnosis of E. granulosus in experimentally infected dogs. A liver with an obvious hydatid cyst was
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Alleviating lesions of chronic progressive lymphedema in Belgian draft horses by successfully treating Chorioptes bovis infestation with moxidectin 0.5% pour-on Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-10 Marieke Brys, Edwin Claerebout, Koen Chiers
The aim of this prospective, randomized, single-blinded, and placebo-controlled clinical trial was to investigate the efficacy of a moxidectin pour-on solution for the treatment of Chorioptes bovis infestation in Belgian draft horses, and in addition, to evaluate the effect of this treatment on the clinical signs and lesions associated with chronic progressive lymphedema (CPL). Nineteen privately owned
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Transcriptome profiling of Madin-Darby bovine kidney cells uncover differences in the susceptibility of cattle to Toxoplasma gondii and Neospora caninum Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-04 Xiaojin Li, Kai He, Weifeng Qian, Chen Li, Suhui Hu, Min Zhang, Tianqi Wang, Wenchao Yan, Meng Qi
Toxoplasma gondii and Neospora caninum are two major apicomplexan protozoan parasites with heteroxenous life cycles and worldwide distributions. The transplacental transmission of N. caninum causes bovine abortion, which resulting in serious economic losses to the dairy industry. Although T. gondii was also reported to cause abortions in pregnant woman and small ruminants, scarce cases about the symptom
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Dose confirmation of a novel fixed dose combination injectable (0.2 mg/kg doramectin + 6.0 mg/kg levamisole hydrochloride) against naturally acquired gastrointestinal nematodes in US cattle Vet. Parasitol. (IF 2.6) Pub Date : 2023-11-05 Andrew A. DeRosa, Angela N. Nadrasik, Jezaniah K. Tena
Macrocyclic lactone (ML) resistance in cattle gastrointestinal nematodes (GINs) is an increasing problem. Concurrent combination anthelmintic therapy incorporating an existing ML with a second drug class has been proposed to control cattle GINs while slowing the development of ML resistance. Two dose confirmation studies were conducted to investigate the efficacy of a new fixed-dose combination injectable